Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0008343

Physical Location & Seq

Genomic Coordinates Forward (+)

15899503 .. 15908551

Gene Structure 9049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2808 bp

ATGCAACGTGGAAAACTTTGCAGCTTAGCTCCTTCTCAGAGCTTCCACTCCCCTCCACTAACTACACGAAGAACCGAGCACATACTCTTCTGCAAAAATCTCCAAACCTCTACAAAAACCTCCTCCATTTCTCCACAAAATTACATATCTGCCGCCACCACCCTCTCTCTTCCACTCTGCAACTCAGCCATGGCGTCTCTGCAAACCCACCATTACCATCTTCAACTCCACCGCCGCTGCGTCTCCTCTCTCACTGCCCCCAAACTCCGCAACCTTCGATTCCCCAACCTTTTTCTGCGCTTCCCGGCTTCATTGCCCGGGAAAGTTAGGGTTTTTACTCTCAGAGGAAATGGGAGGGGCAAGGATGGAATTTACTCGTTTTGCTGCGTTTGCAGAGCTGGCGCTGAGATTGAAAAGGTTGATGGTGAGGAAGGAAGTGAAAGGCCTCCTTTTGATATTAATTTGGCAGTGGTTCTTGCAGGTTTTGCTTTCGAGGCCTACTCGAGCCCTCCTGATAATGTTGGAAGGCATGAAGTTGATGCTGCAGATTGTAAGACAGTGTATCTTTCAGAGTCATTTATACGTGAGATCTATGATGGCGAATTATCTGTTAAACTGAAAAAAGGTCTCCAGTTACCAGCCATGGATCCATGGGGAACAAGTGATCCATATGTAATTATGACATTAGATGGTCAAGTCGTAAAAAGCAAGGTTAAATGGGGGACAAAAGAGCCAACATGGAACGAGGACTTCAGTTTTAATATCAAGCTGCCTCCTACCAAAAATCTTCAGGTTGCAGCTTGGGATGCAAACCTTGTAAGTCCACATAAACGAATGGGGAATGCGAGCATCAGTCTAGAAGACCTTTGTGATGGAAATTTGCATGAAGTACTGGTGGAATTGGAAGGAATGGGAGGCGGTGGAAAAGTACTTTTAGAGGTCAACTATAAAACTTTTGATGAAATTGATGAGGCAAAAAAGTGGTGGAGTAGGGTCCCCTTTGTTTCCGACTTTCTCCGTAAAAATGGTTTTGAACCTGCTATGAAAAAAATGTTTGCTGGCTCGGACACTGTGCAAGCACGTCAATTTGTGGAATATGCTTTTGGGCAGCTAAAGTCATTCAATAATGCAAACCTTATGAAGAATTTAATTGCAAGCGGTGATGTGAATGACACAAAAGGCACTGGGAAATCTAATTCTGCTGGTGTGTCAGATGTGACTTCCCAGATGGGTAGCGTAGCAGAAGGTTTTTTAGATAATGCAGGCTTCAATGCAAGCAGCAATGTAGACGAGTCCAGCATAGATAATGGTGGTGTGGAGAATGGACATACCCCTGAACCACTGAAACAACTTGGTGAAGAAACACAATCAGATAAGAATTTCTGGAAGAAATTTGCCAATGAAATCAATCAAAATGTTGCCGAGAAATTTGGTCTGCCAGTTCCAGAGAAATTAAAGTGGGATGGGTTTGATATATTAAACAGATTTGGTTTGCAGTCACGAGAGATAGCAGAAGCATCTTATATTGAATCAGGGCTTGCAACTCCAGAAGGCTTAGATGTTGATAAGGATAAAACAACTAGCCCACTTTCCATTAGCATGATTCAGTCTTCACTTCCAGATATTAAGAAAGCAACAAGGGATCTATTAAAGCAAACTGATTCTGTTTTAGGAACATTTGTGGTTCTAACTGCTGCAGTATCTGAATCAAACACAGAATCAAATGCGGTAGGAACGAGTGAAACTAAAGTAGAGGATAGTAGCAATGTAGAAGATGATGCCTTGACTGATCCCACAACCGAGGAGATTGCTAGCGCACGGGCTGCAGAGGAGATGAAAGAACTCTTTTCTAGTGCAGAAAGTGCCATGGAGGCTTGGGCGATGCTTGCTACTTCTTTGGGACACCCAAGTTTCATAAAGTCCGAGTTTGAGAAACTATGTTTCTTAGATAATGCAACAACAGACACACAGGTGGCAGTTTGGCGTGATTCTGCTCGGAAAAGACTAGTTATTGCTTTCAGGGGAACAGAACAGTCAAGATGGAAGGACTTGCGAACAGATCTGATGGTAGCTCCTACTGGGCTAAATCCAGAAAGAATAGGCGGAGACTTCAAACAAGAAGTTCAAGTTCATAGTGGTTTCTTAAGGGCATATGATTCTGTCAGGATAAGGATCGTCTCTCTCATGAAATTGGCAATTGGTTATTTTGATGATATTGCTGAGCCGCTGGACAAATGGCATGTTTATATAACGGGTCATAGTCTAGGTGGTGCATTGTCGACCCTCCTGGCTCTTGAACTTTCCTCAAGTCAGCTAGCAAAGCGTGGACTGATTTCTGTGACCATGTATAACTTCGGATCTCCTAGGGTTGGTAACAAAGTATTTGCTGAAATTTATAACGAGAAAGTCAAAGATAGCTGGAGAGTTGTAAACCACAGAGACATCATCCCTACAATCCCCCGCCTGATGGGATACTGCCATGTAGCTCAACCTGTTTACCTAGCAACAGGAGACCTAAGAAATGCCTTGGGAAATATGGAGCTCTCTGAAGATGGTTACCAAGCTGATGTGATTGGGGAGTACACACCAGATGTACTTGTCGGTGAATTCATGAAGGGTGAGAAGGAGCTTATCGAGAAAATATTACAGACCGAAATAAACATATTCCGTTCAGTCAGAGATGGGACTGCACTTATGCAGCACATGGAGGATTTTTACTACATCACATTACTAGAGAATGTGAGATCGAATTACCAAGTTGCAGCAAGGACGCTGTCCGAAGAACAAGTGAATGCGAAAGTTAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0007154 GO:0007165 GO:0008021 GO:0008150 GO:0009987 GO:0010646 GO:0012505 GO:0012506 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030100 GO:0030133 GO:0030334 GO:0030336 GO:0030658 GO:0030659 GO:0030672 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032879 GO:0035556 GO:0040012 GO:0040013 GO:0042175 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044456 GO:0044464 GO:0045202 GO:0045806 GO:0046872 GO:0046928 GO:0048167 GO:0048168 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0051046 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051270 GO:0051271 GO:0051588 GO:0051716 GO:0055037 GO:0060341 GO:0060627 GO:0065007 GO:0065008 GO:0070382 GO:0080134 GO:0097458 GO:0097708 GO:0098588 GO:0098793 GO:0098805 GO:0098827 GO:0099177 GO:0099501 GO:0099503 GO:1902882 GO:1902883 GO:1903530 GO:2000145 GO:2000146

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 Lipase_3

Protein Analysis

936

Amino Acids

103.44

Weight (kDa)

5.43

Isoelectric Point (pI)

41.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	200 - 296	1.4e-19	C2 domain
Lipase_3	PF01764	670 - 821	6.2e-28	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008343)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13550 AT4G13550 AT4G13550
fragaria_vesca	FvH4_4g05250 FvH4_4g05250
malus_domestica	MD13G1226600.v1.1
prunus_persica	Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1
pyrus_communis	pycom13g19960
rosa_chinensis	RchiOBHm_Chr4g0396161
rosa_laevigata	RLG00000009564
rosa_multiflora	Rmu_sc0006325.1_g000041
rosa_roxburghii	Rroxscaffold_5G00341010
rosa_rugosa	Rorug03G0351200 Rorug03G0351300 Rorug03G0351300
rosa_samantha	Rh4BG065200 Rh4CG072000 Rh4DG062000
rosa_wichuraiana	Rw4G005390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2397
Acc36I	ACCTGC	2 cut(s)	470, 1045
AccI	GTMKAC	2 cut(s)	1287, 2279
AclWI	GGATC	7 cut(s)	641, 654, 659, 1650, 1784, 2180, 2365
AcsI	RAATTY	8 cut(s)	369, 877, 1144, 1378, 1391, 1427, 2391, 2606
AcuI	CTGAAG	3 cut(s)	736, 773, 2568
AcyI	GRCGYC	1 cut(s)	194
AfaI	GTAC	4 cut(s)	891, 930, 2582, 2595
AfiI	CCNNNNNNNGG	2 cut(s)	2369, 2467
AflII	CTTAAG	1 cut(s)	2143
AgsI	TTSAA	9 cut(s)	224, 413, 1034, 1123, 1270, 1529, 2113, 2126, 2297
AhlI	ACTAGT	1 cut(s)	2005
AjiI	CACGTC	1 cut(s)	1082
AjnI	CCWGG	1 cut(s)	2286
AjuI	GAANNNNNNNTTGG	2 cut(s)	1086, 1118
AleI	CACNNNNGTG	1 cut(s)	1970
AloI	GAACNNNNNNTCC	2 cut(s)	649, 681
Alw21I	GWGCWC	2 cut(s)	81, 2544
Alw26I	GTCTC	7 cut(s)	201, 247, 632, 2100, 2182, 2433, 2505
AlwI	GGATC	7 cut(s)	641, 654, 659, 1650, 1784, 2180, 2365
AlwNI	CAGNNNCTG	1 cut(s)	1703
Ama87I	CYCGRG	2 cut(s)	317, 502
AoxI	GGCC	2 cut(s)	443, 495
ApoI	RAATTY	8 cut(s)	369, 877, 1144, 1378, 1391, 1427, 2391, 2606
AseI	ATTAAT	1 cut(s)	459
AspA2I	CCTAGG	1 cut(s)	2363
AspLEI	GCGC	3 cut(s)	300, 404, 1817
AspS9I	GGNCC	1 cut(s)	994
AsuC2I	CCSGG	3 cut(s)	305, 318, 319
AsuHPI	GGTGA	5 cut(s)	437, 1172, 1367, 2615, 2630
AsuNHI	GCTAGC	2 cut(s)	1811, 2314
AvaI	CYCGRG	2 cut(s)	317, 502
AvaII	GGWCC	1 cut(s)	994
AvrII	CCTAGG	1 cut(s)	2363
BamHI	GGATCC	1 cut(s)	646
BanII	GRGCYC	2 cut(s)	509, 2544
BauI	CACGAG	1 cut(s)	1500
BbsI	GAAGAC	2 cut(s)	867, 1602
Bbv12I	GWGCWC	2 cut(s)	81, 2544
BcgI	CGANNNNNNTGC	2 cut(s)	1790, 1824
BciT130I	CCWGG	1 cut(s)	2288
BciVI	GTATCC	1 cut(s)	2465
BcnI	CCSGG	3 cut(s)	305, 318, 319
BcoDI	GTCTC	7 cut(s)	201, 247, 632, 2100, 2182, 2433, 2505
BcuI	ACTAGT	1 cut(s)	2005
BfmI	CTRYAG	3 cut(s)	543, 1695, 1824
BfoI	RGCGCY	1 cut(s)	405
BfrI	CTTAAG	1 cut(s)	2143
BfuAI	ACCTGC	2 cut(s)	470, 1045
BfuI	GTATCC	1 cut(s)	2465
BglI	GCCNNNNNGGC	1 cut(s)	1871
BglII	AGATCT	2 cut(s)	588, 2059
BlnI	CCTAGG	1 cut(s)	2363
BlpI	GCTNAGC	2 cut(s)	25, 2220
BmcAI	AGTACT	2 cut(s)	891, 930
Bme1390I	CCNGG	4 cut(s)	305, 318, 319, 2288
Bme18I	GGWCC	1 cut(s)	994
BmeT110I	CYCGRG	2 cut(s)	317, 502
BmgBI	CACGTC	1 cut(s)	1082
BmgT120I	GGNCC	1 cut(s)	994
BmiI	GGNNCC	3 cut(s)	648, 995, 996
BmrFI	CCNGG	4 cut(s)	305, 318, 319, 2288
BmrI	ACTGGG	2 cut(s)	1194, 2088
BmsI	GCATC	6 cut(s)	529, 796, 858, 1526, 1768, 1872
BmtI	GCTAGC	2 cut(s)	1815, 2318
BmuI	ACTGGG	2 cut(s)	1194, 2088
BpiI	GAAGAC	2 cut(s)	867, 1602
BplI	GAGNNNNNCTC	2 cut(s)	485, 517
BpmI	CTGGAG	3 cut(s)	614, 1530, 2440
Bpu1102I	GCTNAGC	2 cut(s)	25, 2220
BpuEI	CTTGAG	1 cut(s)	2290
BpuMI	CCSGG	3 cut(s)	305, 318, 319
BsaAI	YACGTR	1 cut(s)	584
BsaBI	GATNNNNATC	1 cut(s)	2171
BsaHI	GRCGYC	1 cut(s)	194
BsaI	GGTCTC	2 cut(s)	632, 2505
BsaJI	CCNNGG	8 cut(s)	189, 317, 642, 650, 1800, 1866, 2363, 2526
Bsc4I	CCNNNNNNNGG	2 cut(s)	2369, 2467
Bse1I	ACTGG	5 cut(s)	631, 897, 1189, 1439, 2083
Bse3DI	GCAATG	3 cut(s)	311, 1288, 1771
Bse8I	GATNNNNATC	1 cut(s)	2171
BseBI	CCWGG	1 cut(s)	2288
BseDI	CCNNGG	8 cut(s)	189, 317, 642, 650, 1800, 1866, 2363, 2526
BseGI	GGATG	4 cut(s)	370, 811, 1468, 2445
BseJI	GATNNNNATC	1 cut(s)	2171
BseLI	CCNNNNNNNGG	2 cut(s)	2369, 2467
BseMI	GCAATG	3 cut(s)	311, 1288, 1771
BseMII	CTCAG	5 cut(s)	50, 198, 355, 396, 2211
BseNI	ACTGG	5 cut(s)	631, 897, 1189, 1439, 2083
BseRI	GAGGAG	4 cut(s)	112, 235, 1817, 1844
BsgI	GTGCAG	2 cut(s)	1875, 2673
BshFI	GGCC	2 cut(s)	445, 497
BsiHKAI	GWGCWC	2 cut(s)	81, 2544
BsiHKCI	CYCGRG	2 cut(s)	317, 502
BsiSI	CCGG	2 cut(s)	305, 318
BslFI	GGGAC	4 cut(s)	736, 980, 1914, 2698
BslI	CCNNNNNNNGG	2 cut(s)	2369, 2467
BsmAI	GTCTC	7 cut(s)	201, 247, 632, 2100, 2182, 2433, 2505
BsmBI	CGTCTC	3 cut(s)	201, 247, 2182
BsmFI	GGGAC	4 cut(s)	736, 980, 1914, 2698
BsmI	GAATGC	2 cut(s)	847, 2797
BsnI	GGCC	2 cut(s)	445, 497
Bso31I	GGTCTC	2 cut(s)	632, 2505
BsoBI	CYCGRG	2 cut(s)	317, 502
Bsp1286I	GDGCHC	3 cut(s)	81, 509, 2544
Bsp143I	GATC	9 cut(s)	588, 646, 664, 1642, 1789, 2059, 2172, 2357, 2744
Bsp1720I	GCTNAGC	2 cut(s)	25, 2220
Bsp19I	CCATGG	4 cut(s)	189, 642, 650, 1866
BspANI	GGCC	2 cut(s)	445, 497
BspCNI	CTCAG	5 cut(s)	49, 197, 354, 397, 2212
BspHI	TCATGA	2 cut(s)	2184, 2610
BspLI	GGNNCC	3 cut(s)	648, 995, 996
BspMAI	CTGCAG	3 cut(s)	547, 1699, 1828
BspMI	ACCTGC	2 cut(s)	470, 1045
BspOI	GCTAGC	2 cut(s)	1815, 2318
BspPI	GGATC	7 cut(s)	641, 654, 659, 1650, 1784, 2180, 2365
BspTI	CTTAAG	1 cut(s)	2143
BspTNI	GGTCTC	2 cut(s)	632, 2505
BsrDI	GCAATG	3 cut(s)	311, 1288, 1771
BsrI	ACTGG	5 cut(s)	631, 897, 1189, 1439, 2083
BssECI	CCNNGG	8 cut(s)	189, 317, 642, 650, 1800, 1866, 2363, 2526
BssMI	GATC	9 cut(s)	588, 646, 664, 1642, 1789, 2059, 2172, 2357, 2744
BssNI	GRCGYC	1 cut(s)	194
BssSI	CACGAG	1 cut(s)	1500
BssT1I	CCWWGG	6 cut(s)	189, 642, 650, 1866, 2363, 2526
Bst2BI	CACGAG	1 cut(s)	1500
Bst2UI	CCWGG	1 cut(s)	2288
Bst4CI	ACNGT	3 cut(s)	559, 1072, 2034
Bst6I	CTCTTC	2 cut(s)	92, 174
BstACI	GRCGYC	1 cut(s)	194
BstAFI	CTTAAG	1 cut(s)	2143
BstAPI	GCANNNNNTGC	3 cut(s)	485, 1823, 1862
BstBAI	YACGTR	1 cut(s)	584
BstDEI	CTNAG	9 cut(s)	25, 36, 184, 341, 405, 1555, 1945, 2220, 2516
BstDSI	CCRYGG	4 cut(s)	189, 642, 650, 1866
BstEII	GGTNACC	1 cut(s)	2555
BstF5I	GGATG	4 cut(s)	370, 811, 1468, 2445
BstH2I	RGCGCY	1 cut(s)	405
BstHHI	GCGC	3 cut(s)	300, 404, 1817
BstKTI	GATC	9 cut(s)	591, 649, 667, 1645, 1792, 2062, 2175, 2360, 2747
BstMAI	GTCTC	7 cut(s)	201, 247, 632, 2100, 2182, 2433, 2505
BstMBI	GATC	9 cut(s)	588, 646, 664, 1642, 1789, 2059, 2172, 2357, 2744
BstNI	CCWGG	1 cut(s)	2288
BstNSI	RCATGY	1 cut(s)	2243
BstPI	GGTNACC	1 cut(s)	2555
BstSCI	CCNGG	4 cut(s)	303, 316, 317, 2286
BstSFI	CTRYAG	3 cut(s)	543, 1695, 1824
BstV2I	GAAGAC	2 cut(s)	867, 1602
BstX2I	RGATCY	5 cut(s)	588, 646, 1642, 2059, 2357
BstYI	RGATCY	5 cut(s)	588, 646, 1642, 2059, 2357
BsuI	GTATCC	1 cut(s)	2465
BsuRI	GGCC	2 cut(s)	445, 497
BtgI	CCRYGG	4 cut(s)	189, 642, 650, 1866
BtgZI	GCGATG	1 cut(s)	1895
BtrI	CACGTC	1 cut(s)	1082
BtsCI	GGATG	4 cut(s)	370, 811, 1468, 2445
BtsI	GCAGTG	2 cut(s)	252, 474
BtsIMutI	CAGTG	6 cut(s)	252, 474, 564, 1068, 1182, 1340
BveI	ACCTGC	2 cut(s)	470, 1045
CaiI	CAGNNNCTG	1 cut(s)	1703
CciI	TCATGA	2 cut(s)	2184, 2610
CfoI	GCGC	3 cut(s)	300, 404, 1817
Cfr13I	GGNCC	1 cut(s)	994
Cfr9I	CCCGGG	1 cut(s)	317
CseI	GACGC	3 cut(s)	183, 229, 2779
Csp6I	GTAC	4 cut(s)	890, 929, 2581, 2594
CviQI	GTAC	4 cut(s)	890, 929, 2581, 2594
DdeI	CTNAG	9 cut(s)	25, 36, 184, 341, 405, 1555, 1945, 2220, 2516
DpnI	GATC	9 cut(s)	590, 648, 666, 1644, 1791, 2061, 2174, 2359, 2746
DpnII	GATC	9 cut(s)	588, 646, 664, 1642, 1789, 2059, 2172, 2357, 2744
Eam1104I	CTCTTC	2 cut(s)	92, 174
EarI	CTCTTC	2 cut(s)	92, 174
EciI	GGCGGA	1 cut(s)	2118
Ecl136II	GAGCTC	1 cut(s)	2542
Eco130I	CCWWGG	6 cut(s)	189, 642, 650, 1866, 2363, 2526
Eco147I	AGGCCT	2 cut(s)	445, 497
Eco24I	GRGCYC	2 cut(s)	509, 2544
Eco31I	GGTCTC	2 cut(s)	632, 2505
Eco47I	GGWCC	1 cut(s)	994
Eco53kI	GAGCTC	1 cut(s)	2542
Eco57I	CTGAAG	3 cut(s)	736, 773, 2568
Eco88I	CYCGRG	2 cut(s)	317, 502
Eco91I	GGTNACC	1 cut(s)	2555
EcoICRI	GAGCTC	1 cut(s)	2542
EcoO109I	RGGNCCY	1 cut(s)	994
EcoO65I	GGTNACC	1 cut(s)	2555
EcoRI	GAATTC	1 cut(s)	2606
EcoRII	CCWGG	1 cut(s)	2286
EcoT14I	CCWWGG	6 cut(s)	189, 642, 650, 1866, 2363, 2526
EcoT38I	GRGCYC	2 cut(s)	509, 2544
ErhI	CCWWGG	6 cut(s)	189, 642, 650, 1866, 2363, 2526
Esp3I	CGTCTC	3 cut(s)	201, 247, 2182
FalI	AAGNNNNNCTT	2 cut(s)	433, 465
FaqI	GGGAC	4 cut(s)	736, 980, 1914, 2698
FauI	CCCGC	1 cut(s)	2468
FauNDI	CATATG	2 cut(s)	670, 2152
FblI	GTMKAC	2 cut(s)	1287, 2279
FokI	GGATG	4 cut(s)	377, 818, 1475, 2432
FriOI	GRGCYC	2 cut(s)	509, 2544
GlaI	GCGC	3 cut(s)	299, 403, 1816
GsuI	CTGGAG	3 cut(s)	614, 1530, 2440
HaeII	RGCGCY	1 cut(s)	405
HaeIII	GGCC	2 cut(s)	445, 497
HapII	CCGG	2 cut(s)	305, 318
HgaI	GACGC	3 cut(s)	183, 229, 2779
HhaI	GCGC	3 cut(s)	300, 404, 1817
Hin1I	GRCGYC	1 cut(s)	194
Hin6I	GCGC	3 cut(s)	298, 402, 1815
HinP1I	GCGC	3 cut(s)	298, 402, 1815
HincII	GTYRAC	2 cut(s)	943, 2280
HindII	GTYRAC	2 cut(s)	943, 2280
HpaII	CCGG	2 cut(s)	305, 318
HphI	GGTGA	5 cut(s)	437, 1172, 1367, 2615, 2630
Hpy166II	GTNNAC	8 cut(s)	824, 943, 1288, 2280, 2327, 2431, 2497, 2583
Hpy8I	GTNNAC	8 cut(s)	824, 943, 1288, 2280, 2327, 2431, 2497, 2583
HpyCH4III	ACNGT	3 cut(s)	559, 1072, 2034
HpyCH4IV	ACGT	3 cut(s)	7, 583, 1081
HpyF3I	CTNAG	9 cut(s)	25, 36, 184, 341, 405, 1555, 1945, 2220, 2516
HpySE526I	ACGT	3 cut(s)	7, 583, 1081
Hsp92I	GRCGYC	1 cut(s)	194
HspAI	GCGC	3 cut(s)	298, 402, 1815
KflI	GGGWCCC	1 cut(s)	994
Kzo9I	GATC	9 cut(s)	588, 646, 664, 1642, 1789, 2059, 2172, 2357, 2744
LmnI	GCTCC	4 cut(s)	34, 2077, 2539, 2626
LweI	GCATC	6 cut(s)	529, 796, 858, 1526, 1768, 1872
MaeII	ACGT	3 cut(s)	7, 583, 1081
MaeIII	GTNAC	6 cut(s)	633, 1216, 1497, 2338, 2372, 2555
MalI	GATC	9 cut(s)	590, 648, 666, 1644, 1791, 2061, 2174, 2359, 2746
MboI	GATC	9 cut(s)	588, 646, 664, 1642, 1789, 2059, 2172, 2357, 2744
MfeI	CAATTG	1 cut(s)	2196
MflI	RGATCY	5 cut(s)	588, 646, 1642, 2059, 2357
MhlI	GDGCHC	3 cut(s)	81, 509, 2544
MlyI	GAGTC	2 cut(s)	581, 1301
MmeI	TCCRAC	2 cut(s)	502, 1032
MslI	CAYNNNNRTG	2 cut(s)	1598, 1970
MspA1I	CMGCKG	2 cut(s)	237, 2227
MspCI	CTTAAG	1 cut(s)	2143
MspI	CCGG	2 cut(s)	305, 318
MspR9I	CCNGG	4 cut(s)	305, 318, 319, 2288
MunI	CAATTG	1 cut(s)	2196
Mva1269I	GAATGC	2 cut(s)	847, 2797
MvaI	CCWGG	1 cut(s)	2288
NciI	CCSGG	3 cut(s)	305, 318, 319
NcoI	CCATGG	4 cut(s)	189, 642, 650, 1866
NdeI	CATATG	2 cut(s)	670, 2152
NdeII	GATC	9 cut(s)	588, 646, 664, 1642, 1789, 2059, 2172, 2357, 2744
NheI	GCTAGC	2 cut(s)	1811, 2314
NlaIV	GGNNCC	3 cut(s)	648, 995, 996
NmeAIII	GCCGAG	1 cut(s)	1447
NmuCI	GTSAC	3 cut(s)	1216, 1497, 2338
NspI	RCATGY	1 cut(s)	2243
OliI	CACNNNNGTG	1 cut(s)	1970
PaeR7I	CTCGAG	1 cut(s)	502
PagI	TCATGA	2 cut(s)	2184, 2610
PceI	AGGCCT	2 cut(s)	445, 497
PctI	GAATGC	2 cut(s)	847, 2797
PfeI	GAWTC	8 cut(s)	279, 1529, 1603, 1661, 1706, 1718, 1988, 2156
PflFI	GACNNNGTC	1 cut(s)	2773
PleI	GAGTC	2 cut(s)	580, 1300
PpsI	GAGTC	2 cut(s)	580, 1300
Ppu21I	YACGTR	1 cut(s)	584
PpuMI	RGGWCCY	1 cut(s)	994
PshBI	ATTAAT	1 cut(s)	459
PsiI	TTATAA	1 cut(s)	2397
Psp124BI	GAGCTC	1 cut(s)	2544
Psp5II	RGGWCCY	1 cut(s)	994
Psp6I	CCWGG	1 cut(s)	2286
PspEI	GGTNACC	1 cut(s)	2555
PspGI	CCWGG	1 cut(s)	2286
PspN4I	GGNNCC	3 cut(s)	648, 995, 996
PspPI	GGNCC	1 cut(s)	994
PspPPI	RGGWCCY	1 cut(s)	994
PspXI	VCTCGAGB	1 cut(s)	502
PstI	CTGCAG	3 cut(s)	547, 1699, 1828
PstNI	CAGNNNCTG	1 cut(s)	1703
PsuI	RGATCY	5 cut(s)	588, 646, 1642, 2059, 2357
PsyI	GACNNNGTC	1 cut(s)	2773
RsaI	GTAC	4 cut(s)	891, 930, 2582, 2595
RsaNI	GTAC	4 cut(s)	890, 929, 2581, 2594
RseI	CAYNNNNRTG	2 cut(s)	1598, 1970
SacI	GAGCTC	1 cut(s)	2544
SalI	GTCGAC	1 cut(s)	2278
Sau3AI	GATC	9 cut(s)	588, 646, 664, 1642, 1789, 2059, 2172, 2357, 2744
Sau96I	GGNCC	1 cut(s)	994
ScaI	AGTACT	2 cut(s)	891, 930
SchI	GAGTC	2 cut(s)	581, 1301
ScrFI	CCNGG	4 cut(s)	305, 318, 319, 2288
SduI	GDGCHC	3 cut(s)	81, 509, 2544
SfaNI	GCATC	6 cut(s)	529, 796, 858, 1526, 1768, 1872
SfcI	CTRYAG	3 cut(s)	543, 1695, 1824
Sfr274I	CTCGAG	1 cut(s)	502
SinI	GGWCC	1 cut(s)	994
SlaI	CTCGAG	1 cut(s)	502
SmaI	CCCGGG	1 cut(s)	319
SmiMI	CAYNNNNRTG	2 cut(s)	1598, 1970
SmlI	CTYRAG	3 cut(s)	502, 2143, 2305
SmoI	CTYRAG	3 cut(s)	502, 2143, 2305
SpeI	ACTAGT	1 cut(s)	2005
SseBI	AGGCCT	2 cut(s)	445, 497
SspI	AATATT	1 cut(s)	2643
SstI	GAGCTC	1 cut(s)	2544
StuI	AGGCCT	2 cut(s)	445, 497
StyD4I	CCNGG	4 cut(s)	303, 316, 317, 2286
StyI	CCWWGG	6 cut(s)	189, 642, 650, 1866, 2363, 2526
TaaI	ACNGT	3 cut(s)	559, 1072, 2034
TaiI	ACGT	3 cut(s)	10, 586, 1084
TaqI	TCGA	6 cut(s)	277, 492, 503, 2279, 2634, 2747
TaqII	GACCGA	1 cut(s)	2666
TatI	WGTACW	4 cut(s)	889, 928, 2580, 2593
TauI	GCSGC	3 cut(s)	155, 237, 2227
TfiI	GAWTC	8 cut(s)	279, 1529, 1603, 1661, 1706, 1718, 1988, 2156
TscAI	CASTG	6 cut(s)	259, 474, 564, 1075, 1189, 1347
TseFI	GTSAC	3 cut(s)	1216, 1497, 2338
Tsp45I	GTSAC	3 cut(s)	1216, 1497, 2338
TspGWI	ACGGA	2 cut(s)	1007, 2657
TspMI	CCCGGG	1 cut(s)	317
TspRI	CASTG	6 cut(s)	259, 474, 564, 1075, 1189, 1347
Tth111I	GACNNNGTC	1 cut(s)	2773
Vha464I	CTTAAG	1 cut(s)	2143
VpaK11BI	GGWCC	1 cut(s)	994
VspI	ATTAAT	1 cut(s)	459
XapI	RAATTY	8 cut(s)	369, 877, 1144, 1378, 1391, 1427, 2391, 2606
XbaI	TCTAGA	1 cut(s)	856
XceI	RCATGY	1 cut(s)	2243
XhoI	CTCGAG	1 cut(s)	502
XmaI	CCCGGG	1 cut(s)	317
XmaJI	CCTAGG	1 cut(s)	2363
XmiI	GTMKAC	2 cut(s)	1287, 2279
ZrmI	AGTACT	2 cut(s)	891, 930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.