pycom13g20240

Hydroxyethylthiazole

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013360
Physical Location & Seq
Forward (+)
16277089 .. 16278794
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGAGACCTAAGTGGTTAACACTAAAAGTTTCATTACTTTCTCCTGGTAAAAACCCCCCAAACATCCGAAACATCTCGCGGCAAAGCAAAAGAGAAATCATGGAGAGCAAGTCCAACTTCAAGGCAGAAGAAGAAGAACCAGAGGCAATCGTGTGGGCCCAGAAAGCGTGGGCCCTACTATCTTCAGTTCGAGCCAAATCACCGCTCATCCAGTGCATCACCAACTACGTGTCAATGGATCTGATGGCCAACACTCTCTTGTCCGCGGGTGCATCACCCGCCATGCTCCACTCCATAGAAGAACTCCCCGACTTCACGCCTCAGGTCCACGCACTCTGCGTCAACGTGGGCACGCTCTCCGCCGACTGGTTACGGGCCATGAAGGTCGCCGCTGAACTCGCGCACAACTCCGGCAAGCCTTGGGTTCTCGACCCAGTTGCCGCCGGTGCCTCAGTTTTCCGGCTGAAGGCTTGTTTGGAGCTTGTGGAGCTTAAGCCTACTGTTATTAGGGGAAATGCGTCCGAGATTATCGCCCTCGCCAATGCTTCTGTGGGACCTACCAAGGGGGCAGACAGTTCCCATGAATCAACAGATGCAATGGAATCAGCAAAATCACTGGCTCAATCCAGTGGTGCCTTAGTTGCGGTTTCTGGAGCTGTCGACATTGTTACAGATGGGAAGAGAGTTGTTGGTGCTCGTAATGGGGTTGCCATGTTGACAAAGATCACGGCAACCGGATGTTCAGTCACTGCCCTGATTGCTGCCTTTGTTGCTGTTGATCAATCGCATGTTTTGGAAGCTACAGCTTCTGCATTATCAATATTTGGGATTGCGAGCGAGGTGGGAATGAAACTGGCAAATGGTCCAGCTTCGTTGCGGATGCACTTGATAGATTCACTCTACAGGCTCGATGAAGTATGCGTGCTTTCTCATGTTAAAATCGGCAGCTTGTCATGA

Protein Analysis

319

Amino Acids

33.53

Weight (kDa)

7.68

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HK PF02110 60 - 304 1.2e-70 Hydroxyethylthiazole kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013360)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24030
fragaria_vesca FvH4_4g05510 FvH4_4g05510
malus_domestica MD13G1229700.v1.1
prunus_persica Prupe.1G056900_v2.0.a1
pyrus_communis pycom13g20240
rosa_chinensis RchiOBHm_Chr4g0396631
rosa_laevigata RLG00000009514
rosa_multiflora Rmu_sc0006586.1_g000020 Rmu_sc0010969.1_g000006
rosa_roxburghii Rroxscaffold_2G00122890 Rroxscaffold_5G00341480
rosa_rugosa Rorug03G0348300
rosa_samantha Rh4AG070800 Rh4BG068800 Rh4CG076600 Rh4DG066000
rosa_wichuraiana Rw4G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 446, 632
AccBSI CCGCTC 1 cut(s) 205
AccI GTMKAC 1 cut(s) 660
AccII CGCG 3 cut(s) 79, 266, 401
AclWI GGATC 1 cut(s) 246
AcoI YGGCCR 1 cut(s) 246
AcuI CTGAAG 2 cut(s) 168, 485
AfiI CCNNNNNNNGG 3 cut(s) 366, 466, 563
AflII CTTAAG 1 cut(s) 491
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 1 cut(s) 121
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AluBI AGCT 7 cut(s) 481, 490, 656, 800, 806, 869, 948
AluI AGCT 7 cut(s) 481, 490, 656, 800, 806, 869, 948
Alw21I GWGCWC 1 cut(s) 697
AlwI GGATC 1 cut(s) 246
AlwNI CAGNNNCTG 2 cut(s) 749, 809
AoxI GGCC 4 cut(s) 156, 171, 246, 375
ApaI GGGCCC 2 cut(s) 160, 175
ApeKI GCWGC 2 cut(s) 761, 945
AspLEI GCGC 1 cut(s) 403
AspS9I GGNCC 8 cut(s) 156, 157, 171, 172, 325, 375, 554, 863
AsuHPI GGTGA 3 cut(s) 192, 211, 267
AvaII GGWCC 3 cut(s) 325, 554, 863
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 3 cut(s) 160, 175, 353
BalI TGGCCA 1 cut(s) 248
BanI GGYRCC 2 cut(s) 446, 632
BanII GRGCYC 2 cut(s) 160, 175
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 1 cut(s) 748
BccI CCATC 2 cut(s) 238, 668
BceAI ACGGC 1 cut(s) 744
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 1 cut(s) 778
BfmI CTRYAG 2 cut(s) 801, 901
BfrI CTTAAG 1 cut(s) 491
BisI GCNGC 5 cut(s) 80, 390, 441, 762, 946
BlsI GCNGC 5 cut(s) 81, 391, 442, 763, 947
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 3 cut(s) 325, 554, 863
BmgT120I GGNCC 8 cut(s) 156, 157, 171, 172, 325, 375, 554, 863
BmiI GGNNCC 5 cut(s) 158, 173, 448, 555, 634
BmrFI CCNGG 1 cut(s) 45
BmrI ACTGGG 1 cut(s) 428
BmsI GCATC 4 cut(s) 225, 281, 583, 870
BmuI ACTGGG 1 cut(s) 428
BpmI CTGGAG 1 cut(s) 672
BsaAI YACGTR 1 cut(s) 229
BsaJI CCNNGG 3 cut(s) 264, 419, 561
BsaWI WCCGGW 1 cut(s) 734
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 3 cut(s) 366, 466, 563
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 6 cut(s) 211, 371, 434, 621, 627, 858
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 264, 419, 561
BseGI GGATG 4 cut(s) 63, 207, 743, 885
BseLI CCNNNNNNNGG 3 cut(s) 366, 466, 563
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 2 cut(s) 335, 465
BseNI ACTGG 6 cut(s) 211, 371, 434, 621, 627, 858
BseSI GKGCMC 3 cut(s) 160, 175, 353
BseXI GCAGC 1 cut(s) 748
Bsh1236I CGCG 3 cut(s) 79, 266, 401
BshFI GGCC 4 cut(s) 158, 173, 248, 377
BshNI GGYRCC 2 cut(s) 446, 632
BsiHKAI GWGCWC 1 cut(s) 697
BsiSI CCGG 4 cut(s) 411, 444, 460, 735
BslFI GGGAC 1 cut(s) 567
BslI CCNNNNNNNGG 3 cut(s) 366, 466, 563
BsmFI GGGAC 1 cut(s) 567
BsnI GGCC 4 cut(s) 158, 173, 248, 377
Bsp120I GGGCCC 2 cut(s) 156, 171
Bsp1286I GDGCHC 4 cut(s) 160, 175, 353, 697
Bsp143I GATC 3 cut(s) 238, 723, 778
BspANI GGCC 4 cut(s) 158, 173, 248, 377
BspCNI CTCAG 2 cut(s) 334, 464
BspFNI CGCG 3 cut(s) 79, 266, 401
BspHI TCATGA 1 cut(s) 953
BspLI GGNNCC 5 cut(s) 158, 173, 448, 555, 634
BspPI GGATC 1 cut(s) 246
BspT107I GGYRCC 2 cut(s) 446, 632
BspTI CTTAAG 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 205
BsrDI GCAATG 1 cut(s) 603
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 6 cut(s) 211, 371, 434, 621, 627, 858
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 3 cut(s) 264, 419, 561
BssMI GATC 3 cut(s) 238, 723, 778
BssT1I CCWWGG 2 cut(s) 419, 561
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 2 cut(s) 502, 575
Bst6I CTCTTC 1 cut(s) 674
BstAFI CTTAAG 1 cut(s) 491
BstBAI YACGTR 1 cut(s) 229
BstC8I GCNNGC 4 cut(s) 353, 416, 835, 923
BstDEI CTNAG 4 cut(s) 9, 321, 451, 637
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 4 cut(s) 63, 207, 743, 885
BstFNI CGCG 3 cut(s) 79, 266, 401
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 3 cut(s) 241, 726, 781
BstMBI GATC 3 cut(s) 238, 723, 778
BstMWI GCNNNNNNNGC 8 cut(s) 164, 278, 398, 446, 487, 641, 758, 770
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 791
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 2 cut(s) 801, 901
BstSLI GKGCMC 3 cut(s) 160, 175, 353
BstUI CGCG 3 cut(s) 79, 266, 401
BstV1I GCAGC 1 cut(s) 748
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
Bsu36I CCTNAGG 1 cut(s) 321
BsuRI GGCC 4 cut(s) 158, 173, 248, 377
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 4 cut(s) 63, 207, 743, 885
BtsI GCAGTG 1 cut(s) 747
BtsIMutI CAGTG 4 cut(s) 218, 614, 634, 747
Cac8I GCNNGC 4 cut(s) 353, 416, 835, 923
CaiI CAGNNNCTG 2 cut(s) 749, 809
CciI TCATGA 1 cut(s) 953
CfoI GCGC 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 8 cut(s) 156, 157, 171, 172, 325, 375, 554, 863
Cfr42I CCGCGG 1 cut(s) 267
CseI GACGC 2 cut(s) 328, 507
CviAII CATG 8 cut(s) 100, 283, 379, 581, 712, 788, 932, 954
DdeI CTNAG 4 cut(s) 9, 321, 451, 637
DpnI GATC 3 cut(s) 240, 725, 780
DpnII GATC 3 cut(s) 238, 723, 778
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 674
EarI CTCTTC 1 cut(s) 674
EciI GGCGGA 1 cut(s) 349
Eco130I CCWWGG 2 cut(s) 419, 561
Eco24I GRGCYC 2 cut(s) 160, 175
Eco47I GGWCC 3 cut(s) 325, 554, 863
Eco57I CTGAAG 2 cut(s) 168, 485
Eco81I CCTNAGG 1 cut(s) 321
EcoO109I RGGNCCY 2 cut(s) 172, 554
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 2 cut(s) 419, 561
EcoT38I GRGCYC 2 cut(s) 160, 175
ErhI CCWWGG 2 cut(s) 419, 561
FaeI CATG 8 cut(s) 103, 286, 382, 584, 715, 791, 935, 957
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FaqI GGGAC 1 cut(s) 567
FatI CATG 8 cut(s) 99, 282, 378, 580, 711, 787, 931, 953
FauI CCCGC 2 cut(s) 259, 286
FbaI TGATCA 1 cut(s) 778
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 5 cut(s) 80, 390, 441, 762, 946
FokI GGATG 4 cut(s) 50, 194, 750, 892
FriOI GRGCYC 2 cut(s) 160, 175
Fsp4HI GCNGC 5 cut(s) 80, 390, 441, 762, 946
GlaI GCGC 1 cut(s) 402
GluI GCNGC 5 cut(s) 80, 390, 441, 762, 946
GsuI CTGGAG 1 cut(s) 672
HaeIII GGCC 4 cut(s) 158, 173, 248, 377
HapII CCGG 4 cut(s) 411, 444, 460, 735
HgaI GACGC 2 cut(s) 328, 507
HhaI GCGC 1 cut(s) 403
Hin1II CATG 8 cut(s) 103, 286, 382, 584, 715, 791, 935, 957
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HincII GTYRAC 4 cut(s) 18, 343, 661, 717
HindII GTYRAC 4 cut(s) 18, 343, 661, 717
HinfI GANTC 3 cut(s) 584, 602, 893
HpaI GTTAAC 1 cut(s) 18
HpaII CCGG 4 cut(s) 411, 444, 460, 735
HphI GGTGA 3 cut(s) 192, 211, 267
Hpy166II GTNNAC 5 cut(s) 18, 328, 343, 661, 717
Hpy188I TCNGA 3 cut(s) 68, 243, 523
Hpy188III TCNNGA 3 cut(s) 428, 651, 954
Hpy8I GTNNAC 5 cut(s) 18, 328, 343, 661, 717
HpyAV CCTTC 2 cut(s) 376, 460
HpyCH4III ACNGT 2 cut(s) 502, 575
HpyCH4IV ACGT 2 cut(s) 228, 345
HpyCH4V TGCA 5 cut(s) 216, 272, 596, 812, 883
HpyF10VI GCNNNNNNNGC 8 cut(s) 164, 278, 398, 446, 487, 641, 758, 770
HpyF3I CTNAG 4 cut(s) 9, 321, 451, 637
HpySE526I ACGT 2 cut(s) 228, 345
Hsp92II CATG 8 cut(s) 103, 286, 382, 584, 715, 791, 935, 957
HspAI GCGC 1 cut(s) 401
Ksp22I TGATCA 1 cut(s) 778
KspAI GTTAAC 1 cut(s) 18
KspI CCGCGG 1 cut(s) 267
Kzo9I GATC 3 cut(s) 238, 723, 778
LmnI GCTCC 4 cut(s) 291, 478, 487, 653
Lsp1109I GCAGC 1 cut(s) 748
LweI GCATC 4 cut(s) 225, 281, 583, 870
MaeII ACGT 2 cut(s) 228, 345
MaeIII GTNAC 3 cut(s) 369, 667, 745
MalI GATC 3 cut(s) 240, 725, 780
MbiI CCGCTC 1 cut(s) 205
MboI GATC 3 cut(s) 238, 723, 778
MboII GAAGA 6 cut(s) 140, 143, 146, 174, 311, 691
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 4 cut(s) 160, 175, 353, 697
MlsI TGGCCA 1 cut(s) 248
MluNI TGGCCA 1 cut(s) 248
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 5 cut(s) 136, 330, 460, 545, 832
Mox20I TGGCCA 1 cut(s) 248
MscI TGGCCA 1 cut(s) 248
MseI TTAA 3 cut(s) 17, 492, 936
Msp20I TGGCCA 1 cut(s) 248
MspA1I CMGCKG 2 cut(s) 266, 392
MspCI CTTAAG 1 cut(s) 491
MspI CCGG 4 cut(s) 411, 444, 460, 735
MspR9I CCNGG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 45
MvnI CGCG 3 cut(s) 79, 266, 401
MwoI GCNNNNNNNGC 8 cut(s) 164, 278, 398, 446, 487, 641, 758, 770
NdeII GATC 3 cut(s) 238, 723, 778
NlaIII CATG 8 cut(s) 103, 286, 382, 584, 715, 791, 935, 957
NlaIV GGNNCC 5 cut(s) 158, 173, 448, 555, 634
NmuCI GTSAC 1 cut(s) 745
NspI RCATGY 1 cut(s) 791
PagI TCATGA 1 cut(s) 953
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PfeI GAWTC 3 cut(s) 584, 602, 893
PkrI GCNGC 5 cut(s) 81, 391, 442, 763, 947
Ppu21I YACGTR 1 cut(s) 229
PpuMI RGGWCCY 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 554
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 5 cut(s) 158, 173, 448, 555, 634
PspOMI GGGCCC 2 cut(s) 156, 171
PspPI GGNCC 8 cut(s) 156, 157, 171, 172, 325, 375, 554, 863
PspPPI RGGWCCY 1 cut(s) 554
PstNI CAGNNNCTG 2 cut(s) 749, 809
PsuI RGATCY 1 cut(s) 238
SacII CCGCGG 1 cut(s) 267
SalI GTCGAC 1 cut(s) 659
SaqAI TTAA 3 cut(s) 17, 492, 936
SatI GCNGC 5 cut(s) 80, 390, 441, 762, 946
Sau3AI GATC 3 cut(s) 238, 723, 778
Sau96I GGNCC 8 cut(s) 156, 157, 171, 172, 325, 375, 554, 863
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 4 cut(s) 160, 175, 353, 697
SfaNI GCATC 4 cut(s) 225, 281, 583, 870
SfcI CTRYAG 2 cut(s) 801, 901
Sfr303I CCGCGG 1 cut(s) 267
SgrAI CRCCGGYG 1 cut(s) 443
SgrBI CCGCGG 1 cut(s) 267
SinI GGWCC 3 cut(s) 325, 554, 863
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
SspI AATATT 1 cut(s) 822
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 2 cut(s) 419, 561
TaaI ACNGT 2 cut(s) 502, 575
TaiI ACGT 2 cut(s) 231, 348
TaqI TCGA 4 cut(s) 190, 429, 660, 909
TauI GCSGC 3 cut(s) 82, 392, 443
TfiI GAWTC 3 cut(s) 584, 602, 893
Tru1I TTAA 3 cut(s) 17, 492, 936
Tru9I TTAA 3 cut(s) 17, 492, 936
TscAI CASTG 4 cut(s) 218, 621, 634, 754
TseFI GTSAC 1 cut(s) 745
TseI GCWGC 2 cut(s) 761, 945
Tsp45I GTSAC 1 cut(s) 745
TspDTI ATGAA 5 cut(s) 21, 395, 597, 863, 927
TspRI CASTG 4 cut(s) 218, 621, 634, 754
Vha464I CTTAAG 1 cut(s) 491
VpaK11BI GGWCC 3 cut(s) 325, 554, 863
XceI RCATGY 1 cut(s) 791
XcmI CCANNNNNNNNNTGG 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.