pycom13g24450

Uncharacterised protein family UPF0066

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002784
Physical Location & Seq
Forward (+)
21579323 .. 21585256
5934 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1206 bp
ATGGCTTACTGTTACAGTACAGATGAATCAAAATGGTTAAGAGCCACCATAACCTTTGCGCTTGCAGCGCTCTCCGCCTCCACCGCCTTTTCTTTGTATTTGTTGAAAAGAAAATCAAGGGATTTGGATTCCAAGATTGGTGAGCTCCAGAAATCTCTCAAGGCCTCCTCAGACATATGTGGTGCCGAACGGCAAGGTCGAATTCGGGCTCAGCAGGCTTTGAGAGAAGCGCTGACGCAACCTAAGTTGGATAGTTTGGAGCTGACTTCTTACCCCATGGCACCTATTGGTGTCATCCAGTCATGCTTCTCTACCAGGAATGGGACCCCTCGACAACCTCTACTTGTTCCTCTTTCCAGGGCCTGTTTGATTTTTAATTCAGCTCGTGTTCCTCCAGCTTCGCTTGAGGGTCTTGGAGAATATTCTCACTGCTGGGTTATATATGTTTTCCATCTAAATACAAATCTGGAGAAGCTTTGGAAGGATCCGACTAAGTCAAGGTTCAAGGCCAAGGTGAGGGTACCAAGACTAAATGGTGAAAGGAGGGGAGTCTTTGCGACACGATCTCCACACAGGCCATGCCCCATTGGACTCACTGTTGCCAAGGTGGAAGCTGTACACAGGAGTATGCTTCTACTCTCTGGTGTGGATTTGGTTGATGGGACGCCAGTACTTGATGTCAAACCTTATCTCCCCTACAGTGACAGCATCCAAGGAGCAAGAGTGCCAAAGTGGTTAACGGGGGACAGTGTTTTAGCAGTAGCTTCTGTTAGTTTCTCGGAGGGCTTCACTTCAGCACTCGCTGATTGCTGGGAAATATCAGGAAAGAATTCACTCTATGCTTCACCAGATGAGTTCCAAAACTTGATCAAGCAGGTGCTTTCATGGGATATTCGCTCATTGTCTCAACGAAATCGACCTCATGATTCCTTCACCAAGTCAGAAAATGGGAAACAATTGAACGAATGCTCAGATTACCACCCCGGTGAAGAAGCTTCTAGCCCTGGAAGCGAACAGCCACCGCAGTCTCCCAGAGACATAATATATCACCTGACTCTAGAAGGATTGGATGTTTCATACAGACTCGATTGTGATGGCAGTGTTGTTGTAGAGAAAGTGACAGAAGCATCGAGCGTGACTAATAGTAGTCAAAAGCGATGTAATTACTCTACATGGGGAGACCATTTTGTGCCCTTTGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0066

Protein Analysis

402

Amino Acids

44.49

Weight (kDa)

8.44

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmO_N PF01980 108 - 235 1e-37 tRNA-methyltransferase O N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 865
Acc36I ACCTGC 1 cut(s) 865
Acc65I GGTACC 1 cut(s) 520
AccB1I GGYRCC 3 cut(s) 182, 280, 520
AciI CCGC 3 cut(s) 75, 84, 1022
AclWI GGATC 2 cut(s) 479, 492
AcsI RAATTY 2 cut(s) 201, 829
AcuI CTGAAG 1 cut(s) 777
AcyI GRCGYC 1 cut(s) 665
AfaI GTAC 4 cut(s) 19, 522, 618, 672
AfeI AGCGCT 2 cut(s) 69, 231
AfiI CCNNNNNNNGG 2 cut(s) 321, 516
AgsI TTSAA 3 cut(s) 106, 505, 961
AjnI CCWGG 3 cut(s) 314, 356, 1003
AleI CACNNNNGTG 1 cut(s) 984
AluBI AGCT 8 cut(s) 145, 262, 383, 398, 475, 614, 764, 995
AluI AGCT 8 cut(s) 145, 262, 383, 398, 475, 614, 764, 995
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 4 cut(s) 909, 1029, 1032, 1173
AlwI GGATC 2 cut(s) 479, 492
AlwNI CAGNNNCTG 1 cut(s) 363
Aor51HI AGCGCT 2 cut(s) 69, 231
AoxI GGCC 4 cut(s) 162, 360, 507, 575
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 2 cut(s) 201, 829
Asp700I GAANNNNTTC 1 cut(s) 829
Asp718I GGTACC 1 cut(s) 520
AspLEI GCGC 3 cut(s) 61, 70, 232
AspS9I GGNCC 2 cut(s) 324, 360
AsuC2I CCSGG 1 cut(s) 984
AsuHPI GGTGA 7 cut(s) 152, 526, 548, 837, 925, 998, 1040
AvaII GGWCC 1 cut(s) 324
BaeGI GKGCMC 1 cut(s) 1194
BamHI GGATCC 1 cut(s) 484
BanI GGYRCC 3 cut(s) 182, 280, 520
BanII GRGCYC 2 cut(s) 147, 211
BauI CACGAG 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 77
BccI CCATC 3 cut(s) 459, 653, 1088
BceAI ACGGC 1 cut(s) 206
BciT130I CCWGG 3 cut(s) 316, 358, 1005
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 1 cut(s) 984
BcoDI GTCTC 4 cut(s) 909, 1029, 1032, 1173
BfaI CTAG 2 cut(s) 999, 1058
BfmI CTRYAG 1 cut(s) 697
BfoI RGCGCY 2 cut(s) 71, 233
BfuAI ACCTGC 1 cut(s) 865
BisI GCNGC 1 cut(s) 66
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 67
BmcAI AGTACT 1 cut(s) 672
Bme1390I CCNGG 4 cut(s) 316, 358, 984, 1005
Bme18I GGWCC 1 cut(s) 324
BmgT120I GGNCC 2 cut(s) 324, 360
BmiI GGNNCC 6 cut(s) 184, 282, 325, 326, 486, 522
BmrFI CCNGG 4 cut(s) 316, 358, 984, 1005
BmsI GCATC 2 cut(s) 717, 1136
BpmI CTGGAG 3 cut(s) 131, 378, 488
Bpu1102I GCTNAGC 1 cut(s) 210
BpuEI CTTGAG 2 cut(s) 143, 425
BpuMI CCSGG 1 cut(s) 984
BsaHI GRCGYC 1 cut(s) 665
BsaI GGTCTC 1 cut(s) 1173
BsaJI CCNNGG 7 cut(s) 276, 357, 510, 603, 712, 982, 1003
BsaXI ACNNNNNCTCC 6 cut(s) 550, 580, 675, 705, 708, 738
Bsc4I CCNNNNNNNGG 2 cut(s) 321, 516
Bse1I ACTGG 2 cut(s) 298, 668
BseBI CCWGG 3 cut(s) 316, 358, 1005
BseDI CCNNGG 7 cut(s) 276, 357, 510, 603, 712, 982, 1003
BseGI GGATG 3 cut(s) 294, 708, 1075
BseLI CCNNNNNNNGG 2 cut(s) 321, 516
BseMII CTCAG 3 cut(s) 183, 224, 984
BseNI ACTGG 2 cut(s) 298, 668
BseRI GAGGAG 1 cut(s) 157
BseSI GKGCMC 1 cut(s) 1194
BseXI GCAGC 1 cut(s) 77
BseYI CCCAGC 2 cut(s) 432, 810
BshFI GGCC 4 cut(s) 164, 362, 509, 577
BshNI GGYRCC 3 cut(s) 182, 280, 520
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 1 cut(s) 984
BslFI GGGAC 3 cut(s) 337, 676, 758
BslI CCNNNNNNNGG 2 cut(s) 321, 516
BsmAI GTCTC 4 cut(s) 909, 1029, 1032, 1173
BsmFI GGGAC 3 cut(s) 337, 676, 758
BsmI GAATGC 1 cut(s) 971
BsnI GGCC 4 cut(s) 164, 362, 509, 577
Bso31I GGTCTC 1 cut(s) 1173
Bsp1286I GDGCHC 3 cut(s) 147, 211, 1194
Bsp1407I TGTACA 1 cut(s) 616
Bsp143I GATC 3 cut(s) 484, 563, 867
Bsp1720I GCTNAGC 1 cut(s) 210
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 3 cut(s) 75, 84, 1022
BspANI GGCC 4 cut(s) 164, 362, 509, 577
BspCNI CTCAG 3 cut(s) 182, 223, 983
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 6 cut(s) 184, 282, 325, 326, 486, 522
BspMI ACCTGC 1 cut(s) 865
BspPI GGATC 2 cut(s) 479, 492
BspT107I GGYRCC 3 cut(s) 182, 280, 520
BspTNI GGTCTC 1 cut(s) 1173
BsrGI TGTACA 1 cut(s) 616
BsrI ACTGG 2 cut(s) 298, 668
BssECI CCNNGG 7 cut(s) 276, 357, 510, 603, 712, 982, 1003
BssMI GATC 3 cut(s) 484, 563, 867
BssNI GRCGYC 1 cut(s) 665
BssSI CACGAG 1 cut(s) 384
BssT1I CCWWGG 4 cut(s) 276, 510, 603, 712
Bst2BI CACGAG 1 cut(s) 384
Bst2UI CCWGG 3 cut(s) 316, 358, 1005
Bst4CI ACNGT 5 cut(s) 11, 17, 598, 701, 749
BstACI GRCGYC 1 cut(s) 665
BstAUI TGTACA 1 cut(s) 616
BstC8I GCNNGC 2 cut(s) 63, 216
BstDEI CTNAG 5 cut(s) 169, 210, 243, 492, 970
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 3 cut(s) 294, 708, 1075
BstH2I RGCGCY 2 cut(s) 71, 233
BstHHI GCGC 3 cut(s) 61, 70, 232
BstKTI GATC 3 cut(s) 487, 566, 870
BstMAI GTCTC 4 cut(s) 909, 1029, 1032, 1173
BstMBI GATC 3 cut(s) 484, 563, 867
BstMWI GCNNNNNNNGC 6 cut(s) 65, 67, 74, 83, 215, 1008
BstNI CCWGG 3 cut(s) 316, 358, 1005
BstSCI CCNGG 4 cut(s) 314, 356, 982, 1003
BstSFI CTRYAG 1 cut(s) 697
BstSLI GKGCMC 1 cut(s) 1194
BstV1I GCAGC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
BsuRI GGCC 4 cut(s) 164, 362, 509, 577
BtgI CCRYGG 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 1171
BtsCI GGATG 3 cut(s) 294, 708, 1075
BtsI GCAGTG 2 cut(s) 427, 1105
BtsIMutI CAGTG 5 cut(s) 427, 594, 706, 754, 1105
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 2 cut(s) 63, 216
CaiI CAGNNNCTG 1 cut(s) 363
CciI TCATGA 1 cut(s) 922
CfoI GCGC 3 cut(s) 61, 70, 232
Cfr13I GGNCC 2 cut(s) 324, 360
CseI GACGC 2 cut(s) 244, 673
Csp6I GTAC 4 cut(s) 18, 521, 617, 671
CviAII CATG 6 cut(s) 277, 303, 579, 885, 923, 1173
CviQI GTAC 4 cut(s) 18, 521, 617, 671
DdeI CTNAG 5 cut(s) 169, 210, 243, 492, 970
DpnI GATC 3 cut(s) 486, 565, 869
DpnII GATC 3 cut(s) 484, 563, 867
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 145
Eco130I CCWWGG 4 cut(s) 276, 510, 603, 712
Eco147I AGGCCT 1 cut(s) 164
Eco24I GRGCYC 2 cut(s) 147, 211
Eco31I GGTCTC 1 cut(s) 1173
Eco47I GGWCC 1 cut(s) 324
Eco47III AGCGCT 2 cut(s) 69, 231
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 777
EcoICRI GAGCTC 1 cut(s) 145
EcoO109I RGGNCCY 2 cut(s) 324, 360
EcoRI GAATTC 2 cut(s) 201, 829
EcoRII CCWGG 3 cut(s) 314, 356, 1003
EcoT14I CCWWGG 4 cut(s) 276, 510, 603, 712
EcoT38I GRGCYC 2 cut(s) 147, 211
ErhI CCWWGG 4 cut(s) 276, 510, 603, 712
FaeI CATG 6 cut(s) 280, 306, 582, 888, 926, 1176
FaqI GGGAC 3 cut(s) 337, 676, 758
FatI CATG 6 cut(s) 276, 302, 578, 884, 922, 1172
FauNDI CATATG 1 cut(s) 176
FbaI TGATCA 1 cut(s) 867
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 3 cut(s) 281, 695, 1082
FriOI GRGCYC 2 cut(s) 147, 211
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 2 cut(s) 999, 1058
GlaI GCGC 3 cut(s) 60, 69, 231
GluI GCNGC 1 cut(s) 66
GsaI CCCAGC 2 cut(s) 436, 814
GsuI CTGGAG 3 cut(s) 131, 378, 488
HaeII RGCGCY 2 cut(s) 71, 233
HaeIII GGCC 4 cut(s) 164, 362, 509, 577
HapII CCGG 1 cut(s) 984
HgaI GACGC 2 cut(s) 244, 673
HhaI GCGC 3 cut(s) 61, 70, 232
Hin1I GRCGYC 1 cut(s) 665
Hin1II CATG 6 cut(s) 280, 306, 582, 888, 926, 1176
Hin6I GCGC 3 cut(s) 59, 68, 230
HinP1I GCGC 3 cut(s) 59, 68, 230
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HindIII AAGCTT 2 cut(s) 473, 993
HinfI GANTC 7 cut(s) 26, 128, 549, 591, 926, 1054, 1083
HpaI GTTAAC 1 cut(s) 738
HpaII CCGG 1 cut(s) 984
HphI GGTGA 7 cut(s) 152, 526, 548, 837, 925, 998, 1040
Hpy166II GTNNAC 2 cut(s) 619, 738
Hpy188I TCNGA 5 cut(s) 172, 489, 781, 943, 973
Hpy188III TCNNGA 5 cut(s) 148, 467, 822, 923, 1058
Hpy8I GTNNAC 2 cut(s) 619, 738
HpyAV CCTTC 3 cut(s) 475, 940, 1055
HpyCH4III ACNGT 5 cut(s) 11, 17, 598, 701, 749
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 67, 74, 83, 215, 1008
HpyF3I CTNAG 5 cut(s) 169, 210, 243, 492, 970
Hsp92I GRCGYC 1 cut(s) 665
Hsp92II CATG 6 cut(s) 280, 306, 582, 888, 926, 1176
HspAI GCGC 3 cut(s) 59, 68, 230
KflI GGGWCCC 1 cut(s) 324
KpnI GGTACC 1 cut(s) 524
Ksp22I TGATCA 1 cut(s) 867
KspAI GTTAAC 1 cut(s) 738
Kzo9I GATC 3 cut(s) 484, 563, 867
LmnI GCTCC 3 cut(s) 150, 259, 716
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 2 cut(s) 717, 1136
MaeI CTAG 2 cut(s) 999, 1058
MaeIII GTNAC 4 cut(s) 11, 701, 1117, 1135
MalI GATC 3 cut(s) 486, 565, 869
MboI GATC 3 cut(s) 484, 563, 867
MboII GAAGA 1 cut(s) 1001
MfeI CAATTG 1 cut(s) 956
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 3 cut(s) 147, 211, 1194
MluCI AATT 5 cut(s) 201, 376, 829, 956, 1162
MlyI GAGTC 4 cut(s) 558, 585, 1048, 1077
MmeI TCCRAC 2 cut(s) 228, 512
MroXI GAANNNNTTC 1 cut(s) 829
MseI TTAA 3 cut(s) 38, 375, 737
MslI CAYNNNNRTG 1 cut(s) 984
MspI CCGG 1 cut(s) 984
MspR9I CCNGG 4 cut(s) 316, 358, 984, 1005
MunI CAATTG 1 cut(s) 956
Mva1269I GAATGC 1 cut(s) 971
MvaI CCWGG 3 cut(s) 316, 358, 1005
MwoI GCNNNNNNNGC 6 cut(s) 65, 67, 74, 83, 215, 1008
NciI CCSGG 1 cut(s) 984
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 176
NdeII GATC 3 cut(s) 484, 563, 867
NlaIII CATG 6 cut(s) 280, 306, 582, 888, 926, 1176
NlaIV GGNNCC 6 cut(s) 184, 282, 325, 326, 486, 522
NmuCI GTSAC 3 cut(s) 701, 1117, 1135
OliI CACNNNNGTG 1 cut(s) 984
PagI TCATGA 1 cut(s) 922
PaqCI CACCTGC 1 cut(s) 865
PceI AGGCCT 1 cut(s) 164
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PctI GAATGC 1 cut(s) 971
PdmI GAANNNNTTC 1 cut(s) 829
PfeI GAWTC 3 cut(s) 26, 128, 926
PflFI GACNNNGTC 1 cut(s) 493
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 4 cut(s) 557, 585, 1048, 1077
PpsI GAGTC 4 cut(s) 557, 585, 1048, 1077
PpuMI RGGWCCY 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 324
Psp6I CCWGG 3 cut(s) 314, 356, 1003
PspFI CCCAGC 2 cut(s) 432, 810
PspGI CCWGG 3 cut(s) 314, 356, 1003
PspN4I GGNNCC 6 cut(s) 184, 282, 325, 326, 486, 522
PspPI GGNCC 2 cut(s) 324, 360
PspPPI RGGWCCY 1 cut(s) 324
PstNI CAGNNNCTG 1 cut(s) 363
PsuI RGATCY 1 cut(s) 484
PsyI GACNNNGTC 1 cut(s) 493
RsaI GTAC 4 cut(s) 19, 522, 618, 672
RsaNI GTAC 4 cut(s) 18, 521, 617, 671
RseI CAYNNNNRTG 1 cut(s) 984
SacI GAGCTC 1 cut(s) 147
SaqAI TTAA 3 cut(s) 38, 375, 737
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 3 cut(s) 484, 563, 867
Sau96I GGNCC 2 cut(s) 324, 360
ScaI AGTACT 1 cut(s) 672
SchI GAGTC 4 cut(s) 558, 585, 1048, 1077
ScrFI CCNGG 4 cut(s) 316, 358, 984, 1005
SduI GDGCHC 3 cut(s) 147, 211, 1194
SfaNI GCATC 2 cut(s) 717, 1136
SfcI CTRYAG 1 cut(s) 697
SinI GGWCC 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 984
SmlI CTYRAG 2 cut(s) 158, 404
SmoI CTYRAG 2 cut(s) 158, 404
Sse9I AATT 5 cut(s) 201, 376, 829, 956, 1162
SseBI AGGCCT 1 cut(s) 164
SsiI CCGC 3 cut(s) 75, 84, 1022
SspI AATATT 1 cut(s) 422
SspMI CTAG 2 cut(s) 999, 1058
SstI GAGCTC 1 cut(s) 147
StuI AGGCCT 1 cut(s) 164
StyD4I CCNGG 4 cut(s) 314, 356, 982, 1003
StyI CCWWGG 4 cut(s) 276, 510, 603, 712
TaaI ACNGT 5 cut(s) 11, 17, 598, 701, 749
TaqI TCGA 5 cut(s) 199, 331, 916, 1086, 1130
TasI AATT 5 cut(s) 201, 376, 829, 956, 1162
TatI WGTACW 3 cut(s) 17, 616, 670
TfiI GAWTC 3 cut(s) 26, 128, 926
Tru1I TTAA 3 cut(s) 38, 375, 737
Tru9I TTAA 3 cut(s) 38, 375, 737
TscAI CASTG 5 cut(s) 434, 601, 706, 754, 1105
TseFI GTSAC 3 cut(s) 701, 1117, 1135
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 3 cut(s) 701, 1117, 1135
TspDTI ATGAA 3 cut(s) 39, 873, 1065
TspRI CASTG 5 cut(s) 434, 601, 706, 754, 1105
Tth111I GACNNNGTC 1 cut(s) 493
VpaK11BI GGWCC 1 cut(s) 324
XapI RAATTY 2 cut(s) 201, 829
XbaI TCTAGA 1 cut(s) 1057
XmnI GAANNNNTTC 1 cut(s) 829
XspI CTAG 2 cut(s) 999, 1058
ZrmI AGTACT 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.