pycom13g24920

BNR repeat-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001718
Physical Location & Seq
Reverse (-)
21898346 .. 21901349
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1206 bp
ATGTGGAAATCAGTGGGAAGCATTGTGTTGCTGTCTTTCTTTCTGGTTTTGGTGTTTTCGGTGCTGAATGTGTTTGATCTGCTGCATCCTCATACTCCTTTTTCCTTCAATTCCACTCTCGCATCCCATGCCCAACACCACCTCCTTCACTACTTTCCCATCGGAAAACTAGATAGAATATTGGCAGAAACCGCAGCGACTGCACCTGCAACCCCGAATGTAAGTATGAGGAAGGACTGTTACCTTGAGGGGCCTGTGTTGGAGGAATACACGTTTCCTGCACATTCTGCTCCCTTCAATAGCTGCCATGCTTCTACCATTGTTGAGGTTGATAAAGATCACTTCTTGGTTGCCTATTTCGGAGGTACGTCAGAGGGTGCACCTGATGTGAAGATTTGGTTGCAGACCTACAAGAATGGCTATTGGCAGTCACCTGTCGTTGCTGATGAACAACTTGACACCCCAATGTGGAACCCTGTATTGTTCAAACTTCCATCAGAGGAGCTGCTGCTGTTCTATAAAATAGGACAAGAGGTTCAAAAATGGAGTGGATGCATGAAGCGGTCATATGATAAAGGTGTTACTTGGACAGTAAGAGAGCAACTTCCTCCTGGTATATTAGGACCAATAAAGAATAAGCCTATCTTGCTGGAAAATGGTAATTTGGTATGTGGATCCTCTGTCGAAAGCTGGAACTCTTGGGGCGCATGGGTGGAGGTCGCAACAGATTCTGTTAGGTCGTGGAGAAAGTACGGTCCTATTTATGTTGAAAACGAAACTCTTAGTGTCATTCAACCAGTTCCTTACCAGACTGCAAAAGGGACCTTGCGCCTTTTAATGCGATCCTTTGATCAGATTGGGAGAGTTTGTGTTGCAGAATCCAACGACGGTGGCCAGAACTGGAATCATGCAATACCTACTGAACTCCCCAATCCAAACTCAGGTATTGATGGGGTAAAGCTAAAAGATGGCTTTATTTTACTTGCTTATAACACGGTCTCAAGGGGTGTACTTAAAGTTGCACTCTCAAATGATGATGGGGACACATGGTGTGATGCTGTGACATTGGAGGAGTCTTTGGGAATGGAATTCTCGTATCCAGCTGTTATTCAGGCCAGTGATGAGTCTGTTCACATCACTTACACTTATAATAGGACACAAATTAAGCATGTTGTCCTTAAGCCAAGATTATGCGAGGACCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BNR_2

Protein Analysis

402

Amino Acids

44.85

Weight (kDa)

5.55

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BNR_2 PF13088 114 - 379 1.8e-81 BNR repeat-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 990, 1149
AarI CACCTGC 1 cut(s) 214
Acc36I ACCTGC 1 cut(s) 214
AciI CCGC 2 cut(s) 192, 562
AclWI GGATC 3 cut(s) 669, 682, 837
AcoI YGGCCR 1 cut(s) 892
AcsI RAATTY 1 cut(s) 1088
AdeI CACNNNGTG 1 cut(s) 1050
AfaI GTAC 3 cut(s) 367, 752, 1011
AfiI CCNNNNNNNGG 2 cut(s) 468, 941
AflII CTTAAG 1 cut(s) 1178
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 6 cut(s) 109, 298, 487, 539, 770, 794
AjnI CCWGG 1 cut(s) 610
AloI GAACNNNNNNTCC 2 cut(s) 519, 551
AluBI AGCT 5 cut(s) 303, 505, 690, 961, 1103
AluI AGCT 5 cut(s) 303, 505, 690, 961, 1103
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 1003
Alw44I GTGCAC 1 cut(s) 378
AlwI GGATC 3 cut(s) 669, 682, 837
AlwNI CAGNNNCTG 2 cut(s) 200, 731
AoxI GGCC 3 cut(s) 251, 892, 1113
ApaLI GTGCAC 1 cut(s) 378
ApeKI GCWGC 5 cut(s) 82, 194, 303, 505, 508
ApoI RAATTY 1 cut(s) 1088
AspLEI GCGC 2 cut(s) 707, 831
AspS9I GGNCC 5 cut(s) 251, 623, 755, 822, 1198
AsuHPI GGTGA 1 cut(s) 423
AvaII GGWCC 4 cut(s) 623, 755, 822, 1198
BaeGI GKGCMC 1 cut(s) 382
BalI TGGCCA 1 cut(s) 894
BamHI GGATCC 1 cut(s) 674
BarI GAAGNNNNNNTAC 2 cut(s) 224, 256
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 5 cut(s) 69, 206, 290, 492, 495
BccI CCATC 5 cut(s) 167, 502, 944, 962, 1031
BciT130I CCWGG 1 cut(s) 612
BciVI GTATCC 1 cut(s) 1107
BclI TGATCA 1 cut(s) 850
BcoDI GTCTC 1 cut(s) 1003
BfaI CTAG 1 cut(s) 170
BfrI CTTAAG 1 cut(s) 1178
BfuAI ACCTGC 1 cut(s) 214
BfuI GTATCC 1 cut(s) 1107
BisI GCNGC 5 cut(s) 83, 195, 304, 506, 509
BlsI GCNGC 5 cut(s) 84, 196, 305, 507, 510
Bme1390I CCNGG 1 cut(s) 612
Bme18I GGWCC 4 cut(s) 623, 755, 822, 1198
BmgT120I GGNCC 5 cut(s) 251, 623, 755, 822, 1198
BmiI GGNNCC 5 cut(s) 252, 473, 676, 823, 1200
BmrFI CCNGG 1 cut(s) 612
BmsI GCATC 4 cut(s) 94, 131, 542, 1045
BpuEI CTTGAG 2 cut(s) 266, 985
BsaBI GATNNNNATC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 1003
BsaXI ACNNNNNCTCC 4 cut(s) 126, 156, 853, 883
Bsc4I CCNNNNNNNGG 2 cut(s) 468, 941
Bse1I ACTGG 3 cut(s) 797, 905, 1116
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 1 cut(s) 612
BseGI GGATG 3 cut(s) 85, 122, 557
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 2 cut(s) 468, 941
BseMII CTCAG 1 cut(s) 954
BseNI ACTGG 3 cut(s) 797, 905, 1116
BseRI GAGGAG 2 cut(s) 515, 1085
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 5 cut(s) 69, 206, 290, 492, 495
BsgI GTGCAG 2 cut(s) 186, 264
BshFI GGCC 3 cut(s) 253, 894, 1115
BsiHKAI GWGCWC 1 cut(s) 382
BslFI GGGAC 2 cut(s) 835, 1055
BslI CCNNNNNNNGG 2 cut(s) 468, 941
BsmAI GTCTC 1 cut(s) 1003
BsmFI GGGAC 2 cut(s) 835, 1055
BsnI GGCC 3 cut(s) 253, 894, 1115
Bso31I GGTCTC 1 cut(s) 1003
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 5 cut(s) 76, 337, 674, 842, 850
BspACI CCGC 2 cut(s) 192, 562
BspANI GGCC 3 cut(s) 253, 894, 1115
BspCNI CTCAG 1 cut(s) 953
BspLI GGNNCC 5 cut(s) 252, 473, 676, 823, 1200
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 3 cut(s) 669, 682, 837
BspTI CTTAAG 1 cut(s) 1178
BspTNI GGTCTC 1 cut(s) 1003
BsrI ACTGG 3 cut(s) 797, 905, 1116
BssMI GATC 5 cut(s) 76, 337, 674, 842, 850
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 5 cut(s) 239, 592, 755, 890, 997
BstAFI CTTAAG 1 cut(s) 1178
BstAPI GCANNNNNTGC 3 cut(s) 128, 200, 287
BstDEI CTNAG 2 cut(s) 782, 940
BstF5I GGATG 3 cut(s) 85, 122, 557
BstHHI GCGC 2 cut(s) 707, 831
BstKTI GATC 5 cut(s) 79, 340, 677, 845, 853
BstMAI GTCTC 1 cut(s) 1003
BstMBI GATC 5 cut(s) 76, 337, 674, 842, 850
BstMWI GCNNNNNNNGC 5 cut(s) 128, 191, 200, 287, 646
BstNI CCWGG 1 cut(s) 612
BstNSI RCATGY 1 cut(s) 1172
BstSCI CCNGG 1 cut(s) 610
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 5 cut(s) 69, 206, 290, 492, 495
BstX2I RGATCY 1 cut(s) 674
BstYI RGATCY 1 cut(s) 674
BsuI GTATCC 1 cut(s) 1107
BsuRI GGCC 3 cut(s) 253, 894, 1115
BtsCI GGATG 3 cut(s) 85, 122, 557
BtsIMutI CAGTG 2 cut(s) 18, 1123
BveI ACCTGC 1 cut(s) 214
CaiI CAGNNNCTG 2 cut(s) 200, 731
CfoI GCGC 2 cut(s) 707, 831
Cfr13I GGNCC 5 cut(s) 251, 623, 755, 822, 1198
Csp6I GTAC 3 cut(s) 366, 751, 1010
CspCI CAANNNNNGTGG 2 cut(s) 871, 906
CviAII CATG 8 cut(s) 128, 308, 556, 708, 908, 1047, 1169, 1203
CviQI GTAC 3 cut(s) 366, 751, 1010
DdeI CTNAG 2 cut(s) 782, 940
DpnI GATC 5 cut(s) 78, 339, 676, 844, 852
DpnII GATC 5 cut(s) 76, 337, 674, 842, 850
DraIII CACNNNGTG 1 cut(s) 1050
EaeI YGGCCR 1 cut(s) 892
Eco31I GGTCTC 1 cut(s) 1003
Eco47I GGWCC 4 cut(s) 623, 755, 822, 1198
EcoO109I RGGNCCY 3 cut(s) 251, 822, 1198
EcoRI GAATTC 1 cut(s) 1088
EcoRII CCWGG 1 cut(s) 610
EcoT22I ATGCAT 1 cut(s) 557
FaeI CATG 8 cut(s) 131, 311, 559, 711, 911, 1050, 1172, 1206
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 2 cut(s) 835, 1055
FatI CATG 8 cut(s) 127, 307, 555, 707, 907, 1046, 1168, 1202
FauNDI CATATG 1 cut(s) 568
FbaI TGATCA 1 cut(s) 850
Fnu4HI GCNGC 5 cut(s) 83, 195, 304, 506, 509
FokI GGATG 3 cut(s) 72, 109, 564
Fsp4HI GCNGC 5 cut(s) 83, 195, 304, 506, 509
FspBI CTAG 1 cut(s) 170
GlaI GCGC 2 cut(s) 706, 830
GluI GCNGC 5 cut(s) 83, 195, 304, 506, 509
HaeIII GGCC 3 cut(s) 253, 894, 1115
HhaI GCGC 2 cut(s) 707, 831
Hin1II CATG 8 cut(s) 131, 311, 559, 711, 911, 1050, 1172, 1206
Hin6I GCGC 2 cut(s) 705, 829
HinP1I GCGC 2 cut(s) 705, 829
HinfI GANTC 5 cut(s) 728, 878, 904, 1073, 1124
HphI GGTGA 1 cut(s) 423
Hpy166II GTNNAC 3 cut(s) 380, 1010, 1132
Hpy188I TCNGA 5 cut(s) 164, 362, 373, 499, 855
Hpy8I GTNNAC 3 cut(s) 380, 1010, 1132
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 4 cut(s) 115, 155, 226, 304
HpyCH4III ACNGT 5 cut(s) 239, 592, 755, 890, 997
HpyCH4IV ACGT 2 cut(s) 272, 368
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 191, 200, 287, 646
HpyF3I CTNAG 2 cut(s) 782, 940
HpySE526I ACGT 2 cut(s) 272, 368
Hsp92II CATG 8 cut(s) 131, 311, 559, 711, 911, 1050, 1172, 1206
HspAI GCGC 2 cut(s) 705, 829
Ksp22I TGATCA 1 cut(s) 850
Kzo9I GATC 5 cut(s) 76, 337, 674, 842, 850
LmnI GCTCC 2 cut(s) 295, 502
Lsp1109I GCAGC 5 cut(s) 69, 206, 290, 492, 495
LweI GCATC 4 cut(s) 94, 131, 542, 1045
MaeI CTAG 1 cut(s) 170
MaeII ACGT 2 cut(s) 272, 368
MaeIII GTNAC 4 cut(s) 239, 429, 580, 1060
MalI GATC 5 cut(s) 78, 339, 676, 844, 852
MboI GATC 5 cut(s) 76, 337, 674, 842, 850
MboII GAAGA 1 cut(s) 403
MflI RGATCY 1 cut(s) 674
MhlI GDGCHC 1 cut(s) 382
MlsI TGGCCA 1 cut(s) 894
MluCI AATT 4 cut(s) 109, 661, 1088, 1161
MluNI TGGCCA 1 cut(s) 894
MlyI GAGTC 2 cut(s) 1082, 1133
MmeI TCCRAC 2 cut(s) 240, 906
Mox20I TGGCCA 1 cut(s) 894
Mph1103I ATGCAT 1 cut(s) 557
MscI TGGCCA 1 cut(s) 894
MseI TTAA 4 cut(s) 836, 1014, 1164, 1179
MslI CAYNNNNRTG 1 cut(s) 464
Msp20I TGGCCA 1 cut(s) 894
MspA1I CMGCKG 1 cut(s) 1103
MspCI CTTAAG 1 cut(s) 1178
MspR9I CCNGG 1 cut(s) 612
MvaI CCWGG 1 cut(s) 612
MwoI GCNNNNNNNGC 5 cut(s) 128, 191, 200, 287, 646
NdeI CATATG 1 cut(s) 568
NdeII GATC 5 cut(s) 76, 337, 674, 842, 850
NlaIII CATG 8 cut(s) 131, 311, 559, 711, 911, 1050, 1172, 1206
NlaIV GGNNCC 5 cut(s) 252, 473, 676, 823, 1200
NmuCI GTSAC 2 cut(s) 429, 1060
NsiI ATGCAT 1 cut(s) 557
NspI RCATGY 1 cut(s) 1172
PaqCI CACCTGC 1 cut(s) 214
PfeI GAWTC 3 cut(s) 728, 878, 904
PkrI GCNGC 5 cut(s) 84, 196, 305, 507, 510
PleI GAGTC 2 cut(s) 1081, 1132
PpsI GAGTC 2 cut(s) 1081, 1132
PpuMI RGGWCCY 2 cut(s) 822, 1198
PsiI TTATAA 2 cut(s) 990, 1149
Psp5II RGGWCCY 2 cut(s) 822, 1198
Psp6I CCWGG 1 cut(s) 610
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 5 cut(s) 252, 473, 676, 823, 1200
PspPI GGNCC 5 cut(s) 251, 623, 755, 822, 1198
PspPPI RGGWCCY 2 cut(s) 822, 1198
PstNI CAGNNNCTG 2 cut(s) 200, 731
PsuI RGATCY 1 cut(s) 674
PvuII CAGCTG 1 cut(s) 1103
RsaI GTAC 3 cut(s) 367, 752, 1011
RsaNI GTAC 3 cut(s) 366, 751, 1010
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 4 cut(s) 836, 1014, 1164, 1179
SatI GCNGC 5 cut(s) 83, 195, 304, 506, 509
Sau3AI GATC 5 cut(s) 76, 337, 674, 842, 850
Sau96I GGNCC 5 cut(s) 251, 623, 755, 822, 1198
SchI GAGTC 2 cut(s) 1082, 1133
ScrFI CCNGG 1 cut(s) 612
SduI GDGCHC 1 cut(s) 382
SfaNI GCATC 4 cut(s) 94, 131, 542, 1045
SinI GGWCC 4 cut(s) 623, 755, 822, 1198
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 3 cut(s) 245, 1000, 1178
SmoI CTYRAG 3 cut(s) 245, 1000, 1178
Sse9I AATT 4 cut(s) 109, 661, 1088, 1161
SsiI CCGC 2 cut(s) 192, 562
SspI AATATT 1 cut(s) 180
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 610
TaaI ACNGT 5 cut(s) 239, 592, 755, 890, 997
TaiI ACGT 2 cut(s) 275, 371
TaqI TCGA 1 cut(s) 684
TasI AATT 4 cut(s) 109, 661, 1088, 1161
TatI WGTACW 1 cut(s) 1009
TfiI GAWTC 3 cut(s) 728, 878, 904
Tru1I TTAA 4 cut(s) 836, 1014, 1164, 1179
Tru9I TTAA 4 cut(s) 836, 1014, 1164, 1179
TscAI CASTG 2 cut(s) 18, 1123
TseFI GTSAC 2 cut(s) 429, 1060
TseI GCWGC 5 cut(s) 82, 194, 303, 505, 508
Tsp45I GTSAC 2 cut(s) 429, 1060
TspDTI ATGAA 2 cut(s) 462, 572
TspRI CASTG 2 cut(s) 18, 1123
Vha464I CTTAAG 1 cut(s) 1178
VneI GTGCAC 1 cut(s) 378
VpaK11BI GGWCC 4 cut(s) 623, 755, 822, 1198
XapI RAATTY 1 cut(s) 1088
XceI RCATGY 1 cut(s) 1172
XspI CTAG 1 cut(s) 170
Zsp2I ATGCAT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.