pycom13g26460

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015716
Physical Location & Seq
Forward (+)
23071447 .. 23072732
1286 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1020 bp
ATGATGGGTCATTATTTAAGTTGGGCTTCAGACCTTGAGAAGGGACATGGATCCTCTTCGGATCTAGTTGTCTTAATCCACCTAGTCAAATCATCTGGGCTATTGAAATTTGATCCAACGCCGTTAGATCAAATTTCAATGGCCCTGGATGATTTGACTAGGTGGATTAGGACAACTGGATCCGAAGAGGATCCATGCCCCTTGAGAAGGTATGATGGGTCAACATTTAAGCTAGCGTTGGGCTTCTCCTTCCTTAGGCCTGACTGCCTAACCAAAACAAAAACACAGAAACAAAAAAGAAAATGGAGGGCCTTCTCTTCTTTGGGCCCAACAACCGTCATAAATATATATATATATATATATATAGATATATATGAGAAAGGAGTTTTTGGGCTACACCTCATTCGGCAACTCTTCTCACCCGAAGACTTGGGGGACTTCTACCATATGCTACTACACCTCGGTACTCAGAAGTTTCATGATTACTCAGTGACTTGGATTTGTCAAGTCCCCAATCGAGAAGCTTTCCTCACTCGGGAACTTAAGGGAGCACTGTTTGTACCATACTTGCCCAATCTCGAAACTCCCGAGCACCGGTCAATGTCATACCTTCAAAGACCCACTGAGGGATTCATGTTGAGACACCCTTGTCGAAGCACAAGAGTCAACGTCAGAATAAAGCTCATAGAGTCCCACATCAATCGTGAACTCCTCTACTTCATGGACATAGCCAAACTTAGGGTGAACCACGTACATCTTGTGTTTGCTTCCCTATCTCTATCTCTTTATATATTATCCTCACTAGGTAACTGGAGCAACCGACTGAAGGTCACAAACTTGACACTTTACGTTGAGTTCCTCGAGATATGTGACACTTTCAAGATCCATCATGTGCCAAGTGAGGCTATCAGATTACACATGTTCTCATTCTCCCTAAGAGACAAGGTGAAGAATTGGCTCCATTCACTTCCACCCAATTTGAGAACAACTTGGAATGATTTGGCCAACAATTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 RVT_1 Retrotrans_gag rve

Protein Analysis

340

Amino Acids

39.5

Weight (kDa)

9.17

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 648
AclWI GGATC 9 cut(s) 45, 58, 69, 107, 174, 185, 187, 198, 877
AcoI YGGCCR 1 cut(s) 1002
AcsI RAATTY 2 cut(s) 107, 132
AcuI CTGAAG 2 cut(s) 12, 845
AfaI GTAC 3 cut(s) 466, 561, 753
AfiI CCNNNNNNNGG 8 cut(s) 40, 207, 255, 535, 594, 626, 738, 826
AflII CTTAAG 1 cut(s) 542
AflIII ACRYGT 1 cut(s) 918
AgeI ACCGGT 1 cut(s) 594
AgsI TTSAA 4 cut(s) 106, 138, 614, 880
AhdI GACNNNNNGTC 1 cut(s) 827
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 3 cut(s) 232, 524, 682
AluI AGCT 3 cut(s) 232, 524, 682
Alw21I GWGCWC 2 cut(s) 553, 594
Alw26I GTCTC 2 cut(s) 634, 933
AlwI GGATC 9 cut(s) 45, 58, 69, 107, 174, 185, 187, 198, 877
Ama87I CYCGRG 3 cut(s) 534, 587, 860
AoxI GGCC 5 cut(s) 141, 257, 309, 325, 1002
ApaI GGGCCC 1 cut(s) 329
ApoI RAATTY 2 cut(s) 107, 132
AsiGI ACCGGT 1 cut(s) 594
AspS9I GGNCC 4 cut(s) 142, 309, 325, 326
AsuHPI GGTGA 3 cut(s) 411, 754, 958
AsuNHI GCTAGC 1 cut(s) 232
AvaI CYCGRG 3 cut(s) 534, 587, 860
AxyI CCTNAGG 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 1004
BamHI GGATCC 3 cut(s) 50, 179, 190
BanII GRGCYC 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 553, 594
BccI CCATC 2 cut(s) 209, 894
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 146
BcoDI GTCTC 2 cut(s) 634, 933
BfaI CTAG 5 cut(s) 65, 83, 159, 233, 803
BfrI CTTAAG 1 cut(s) 542
Bme1390I CCNGG 1 cut(s) 146
BmeRI GACNNNNNGTC 1 cut(s) 827
BmeT110I CYCGRG 3 cut(s) 534, 587, 860
BmgT120I GGNCC 4 cut(s) 142, 309, 325, 326
BmiI GGNNCC 5 cut(s) 52, 181, 192, 327, 959
BmrFI CCNGG 1 cut(s) 146
BmtI GCTAGC 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 432
BpmI CTGGAG 1 cut(s) 832
BpuEI CTTGAG 2 cut(s) 56, 223
BsaAI YACGTR 1 cut(s) 751
BsaJI CCNNGG 2 cut(s) 144, 460
BsaWI WCCGGW 1 cut(s) 594
Bsc4I CCNNNNNNNGG 8 cut(s) 40, 207, 255, 535, 594, 626, 738, 826
Bse118I RCCGGY 1 cut(s) 594
Bse1I ACTGG 2 cut(s) 181, 815
Bse21I CCTNAGG 1 cut(s) 254
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 144, 460
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 8 cut(s) 40, 207, 255, 535, 594, 626, 738, 826
BseMII CTCAG 3 cut(s) 482, 501, 615
BseNI ACTGG 2 cut(s) 181, 815
BseRI GAGGAG 1 cut(s) 701
BseSI GKGCMC 1 cut(s) 329
BshFI GGCC 5 cut(s) 143, 259, 311, 327, 1004
BshTI ACCGGT 1 cut(s) 594
BsiHKAI GWGCWC 2 cut(s) 553, 594
BsiHKCI CYCGRG 3 cut(s) 534, 587, 860
BsiSI CCGG 1 cut(s) 595
BslFI GGGAC 4 cut(s) 57, 449, 494, 676
BslI CCNNNNNNNGG 8 cut(s) 40, 207, 255, 535, 594, 626, 738, 826
BsmAI GTCTC 2 cut(s) 634, 933
BsmFI GGGAC 4 cut(s) 57, 449, 494, 676
BsnI GGCC 5 cut(s) 143, 259, 311, 327, 1004
BsoBI CYCGRG 3 cut(s) 534, 587, 860
Bsp120I GGGCCC 1 cut(s) 325
Bsp1286I GDGCHC 3 cut(s) 329, 553, 594
Bsp143I GATC 7 cut(s) 50, 61, 112, 127, 179, 190, 882
BspANI GGCC 5 cut(s) 143, 259, 311, 327, 1004
BspCNI CTCAG 3 cut(s) 481, 500, 616
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 5 cut(s) 52, 181, 192, 327, 959
BspOI GCTAGC 1 cut(s) 236
BspPI GGATC 9 cut(s) 45, 58, 69, 107, 174, 185, 187, 198, 877
BspTI CTTAAG 1 cut(s) 542
BsrFI RCCGGY 1 cut(s) 594
BsrI ACTGG 2 cut(s) 181, 815
BssAI RCCGGY 1 cut(s) 594
BssECI CCNNGG 2 cut(s) 144, 460
BssMI GATC 7 cut(s) 50, 61, 112, 127, 179, 190, 882
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 337, 555
Bst6I CTCTTC 4 cut(s) 61, 180, 322, 419
BstAFI CTTAAG 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 751
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 6 cut(s) 254, 468, 487, 624, 737, 935
BstENI CCTNNNNNAGG 3 cut(s) 38, 205, 253
BstF5I GGATG 1 cut(s) 154
BstKTI GATC 7 cut(s) 53, 64, 115, 130, 182, 193, 885
BstMAI GTCTC 2 cut(s) 634, 933
BstMBI GATC 7 cut(s) 50, 61, 112, 127, 179, 190, 882
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 922
BstSCI CCNGG 1 cut(s) 144
BstSLI GKGCMC 1 cut(s) 329
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 5 cut(s) 50, 61, 179, 190, 882
BstYI RGATCY 5 cut(s) 50, 61, 179, 190, 882
Bsu36I CCTNAGG 1 cut(s) 254
BsuRI GGCC 5 cut(s) 143, 259, 311, 327, 1004
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 3 cut(s) 495, 551, 621
Cac8I GCNNGC 1 cut(s) 234
CciI TCATGA 1 cut(s) 478
Cfr10I RCCGGY 1 cut(s) 594
Cfr13I GGNCC 4 cut(s) 142, 309, 325, 326
Csp6I GTAC 3 cut(s) 465, 560, 752
CspAI ACCGGT 1 cut(s) 594
CspCI CAANNNNNGTGG 2 cut(s) 960, 995
CviAII CATG 7 cut(s) 47, 195, 479, 634, 721, 890, 919
CviQI GTAC 3 cut(s) 465, 560, 752
DdeI CTNAG 6 cut(s) 254, 468, 487, 624, 737, 935
DpnI GATC 7 cut(s) 52, 63, 114, 129, 181, 192, 884
DpnII GATC 7 cut(s) 50, 61, 112, 127, 179, 190, 882
DrdI GACNNNNNNGTC 1 cut(s) 648
DriI GACNNNNNGTC 1 cut(s) 827
DseDI GACNNNNNNGTC 1 cut(s) 648
EaeI YGGCCR 1 cut(s) 1002
Eam1104I CTCTTC 4 cut(s) 61, 180, 322, 419
Eam1105I GACNNNNNGTC 1 cut(s) 827
EarI CTCTTC 4 cut(s) 61, 180, 322, 419
Eco147I AGGCCT 1 cut(s) 259
Eco24I GRGCYC 1 cut(s) 329
Eco57I CTGAAG 2 cut(s) 12, 845
Eco81I CCTNAGG 1 cut(s) 254
Eco88I CYCGRG 3 cut(s) 534, 587, 860
EcoNI CCTNNNNNAGG 3 cut(s) 38, 205, 253
EcoO109I RGGNCCY 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 329
FaeI CATG 7 cut(s) 50, 198, 482, 637, 724, 893, 922
FalI AAGNNNNNCTT 2 cut(s) 10, 42
FaqI GGGAC 4 cut(s) 57, 449, 494, 676
FatI CATG 7 cut(s) 46, 194, 478, 633, 720, 889, 918
FauNDI CATATG 1 cut(s) 447
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 1 cut(s) 329
FspBI CTAG 5 cut(s) 65, 83, 159, 233, 803
GsuI CTGGAG 1 cut(s) 832
HaeIII GGCC 5 cut(s) 143, 259, 311, 327, 1004
HapII CCGG 1 cut(s) 595
Hin1II CATG 7 cut(s) 50, 198, 482, 637, 724, 893, 922
HincII GTYRAC 2 cut(s) 222, 667
HindII GTYRAC 2 cut(s) 222, 667
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 3 cut(s) 630, 663, 689
HpaII CCGG 1 cut(s) 595
HphI GGTGA 3 cut(s) 411, 754, 958
Hpy166II GTNNAC 4 cut(s) 222, 667, 707, 745
Hpy188I TCNGA 6 cut(s) 31, 61, 184, 471, 674, 911
Hpy188III TCNNGA 8 cut(s) 479, 518, 536, 578, 587, 704, 862, 880
Hpy8I GTNNAC 4 cut(s) 222, 667, 707, 745
HpyAV CCTTC 6 cut(s) 34, 201, 259, 322, 620, 820
HpyCH4III ACNGT 2 cut(s) 337, 555
HpyCH4IV ACGT 3 cut(s) 669, 750, 849
HpyF3I CTNAG 6 cut(s) 254, 468, 487, 624, 737, 935
HpySE526I ACGT 3 cut(s) 669, 750, 849
Hsp92II CATG 7 cut(s) 50, 198, 482, 637, 724, 893, 922
Kzo9I GATC 7 cut(s) 50, 61, 112, 127, 179, 190, 882
LmnI GCTCC 3 cut(s) 548, 813, 963
LpnPI CCDG 7 cut(s) 81, 131, 158, 162, 273, 608, 796
MaeI CTAG 5 cut(s) 65, 83, 159, 233, 803
MaeII ACGT 3 cut(s) 669, 750, 849
MaeIII GTNAC 4 cut(s) 490, 806, 829, 869
MalI GATC 7 cut(s) 52, 63, 114, 129, 181, 192, 884
MboI GATC 7 cut(s) 50, 61, 112, 127, 179, 190, 882
MboII GAAGA 6 cut(s) 48, 197, 309, 406, 437, 961
MflI RGATCY 5 cut(s) 50, 61, 179, 190, 882
MhlI GDGCHC 3 cut(s) 329, 553, 594
MlsI TGGCCA 1 cut(s) 1004
MluCI AATT 5 cut(s) 107, 132, 952, 976, 1009
MluNI TGGCCA 1 cut(s) 1004
MlyI GAGTC 2 cut(s) 672, 698
MmeI TCCRAC 1 cut(s) 140
Mox20I TGGCCA 1 cut(s) 1004
MscI TGGCCA 1 cut(s) 1004
MseI TTAA 4 cut(s) 17, 74, 228, 543
Msp20I TGGCCA 1 cut(s) 1004
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 1 cut(s) 595
MspR9I CCNGG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 146
NdeI CATATG 1 cut(s) 447
NdeII GATC 7 cut(s) 50, 61, 112, 127, 179, 190, 882
NheI GCTAGC 1 cut(s) 232
NlaIII CATG 7 cut(s) 50, 198, 482, 637, 724, 893, 922
NlaIV GGNNCC 5 cut(s) 52, 181, 192, 327, 959
NmuCI GTSAC 3 cut(s) 490, 829, 869
NspI RCATGY 1 cut(s) 922
PaeR7I CTCGAG 1 cut(s) 860
PagI TCATGA 1 cut(s) 478
PceI AGGCCT 1 cut(s) 259
PciI ACATGT 1 cut(s) 918
PcsI WCGNNNNNNNCGW 1 cut(s) 585
PfeI GAWTC 1 cut(s) 630
PinAI ACCGGT 1 cut(s) 594
PleI GAGTC 2 cut(s) 671, 697
PpsI GAGTC 2 cut(s) 671, 697
Ppu21I YACGTR 1 cut(s) 751
PscI ACATGT 1 cut(s) 918
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 5 cut(s) 52, 181, 192, 327, 959
PspOMI GGGCCC 1 cut(s) 325
PspPI GGNCC 4 cut(s) 142, 309, 325, 326
PsuI RGATCY 5 cut(s) 50, 61, 179, 190, 882
RsaI GTAC 3 cut(s) 466, 561, 753
RsaNI GTAC 3 cut(s) 465, 560, 752
SaqAI TTAA 4 cut(s) 17, 74, 228, 543
Sau3AI GATC 7 cut(s) 50, 61, 112, 127, 179, 190, 882
Sau96I GGNCC 4 cut(s) 142, 309, 325, 326
SchI GAGTC 2 cut(s) 672, 698
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 3 cut(s) 329, 553, 594
Sfr274I CTCGAG 1 cut(s) 860
SlaI CTCGAG 1 cut(s) 860
SmlI CTYRAG 4 cut(s) 35, 202, 542, 860
SmoI CTYRAG 4 cut(s) 35, 202, 542, 860
Sse9I AATT 5 cut(s) 107, 132, 952, 976, 1009
SseBI AGGCCT 1 cut(s) 259
SspMI CTAG 5 cut(s) 65, 83, 159, 233, 803
StuI AGGCCT 1 cut(s) 259
StyD4I CCNGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 337, 555
TaiI ACGT 3 cut(s) 672, 753, 852
TaqI TCGA 4 cut(s) 517, 579, 652, 861
TasI AATT 5 cut(s) 107, 132, 952, 976, 1009
TfiI GAWTC 1 cut(s) 630
Tru1I TTAA 4 cut(s) 17, 74, 228, 543
Tru9I TTAA 4 cut(s) 17, 74, 228, 543
TscAI CASTG 3 cut(s) 495, 558, 628
TseFI GTSAC 3 cut(s) 490, 829, 869
Tsp45I GTSAC 3 cut(s) 490, 829, 869
TspDTI ATGAA 3 cut(s) 467, 622, 709
TspRI CASTG 3 cut(s) 495, 558, 628
Vha464I CTTAAG 1 cut(s) 542
XagI CCTNNNNNAGG 3 cut(s) 38, 205, 253
XapI RAATTY 2 cut(s) 107, 132
XceI RCATGY 1 cut(s) 922
XhoI CTCGAG 1 cut(s) 860
XspI CTAG 5 cut(s) 65, 83, 159, 233, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.