pycom14g03170

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010737
Physical Location & Seq
Forward (+)
2453238 .. 2455484
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGTTGGGAAGCTTGCTAACCAGAATTCTTATATTGTTTCTTGGGTATGCATACCCAGCATTTGAGTGCTACAAAACAGTGGAGAAGAACAGAGTTGAGATTGAAGAACTCCGGTTTTGGTGCCAATACTGGATAATCGTGGCAATGCTCACAGTTCTTGAGAGGATTGGAGATGTATTCATCTCATGGTTGCCTATGTATGGTGAAGGGAAGGTGGCACTTTTTATATACCTATGGTATCCAAAGACCAAGGGAACTTTCTTTGTGTACAAGACCTTCCTGAGGCCATATGTAGCAAAGCATGAGACAGACATAGACAGGAAGTTACTGGAGATGAGAGCAAGAGCCTGGGATTTGACGGTATTCTACTGGCAGAACTTCGCCCAATATGGCCACGCTGCTTTCCTCCAAGCCCTTCGGTACATGGCTGCTAACTCCACAAAGTTCGCCACCAAACCCACCACCGAGTCCCAGACAGTCGATGATCGATCAGCTGGATACAGCAGCGCCAGCACCGCCGCTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

176

Amino Acids

20.45

Weight (kDa)

8.98

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 19 - 97 5e-24 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010737)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42560 AT5G42560 AT5G42560
fragaria_vesca FvH4_6g19930
malus_domestica MD12G1036900.v1.1 MD14G1034500.v1.1 MD14G1035900.v1.1
prunus_persica Prupe.7G098200_v2.0.a1
pyrus_communis pycom14g03170
rosa_chinensis RchiOBHm_Chr3g0475331
rosa_laevigata RLG00000023851
rosa_multiflora Rmu_co8097434.1_g000001 Rmu_ssc0000140.1_g000014
rosa_roxburghii Rroxscaffold_6G00406380
rosa_rugosa Rorug03G0148200
rosa_samantha Rh3AG198100 Rh3BG228300 Rh3CG222600 Rh3DG223500
rosa_wichuraiana Rw3G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 120
AciI CCGC 2 cut(s) 516, 519
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 1 cut(s) 24
AfaI GTAC 2 cut(s) 269, 422
AfiI CCNNNNNNNGG 2 cut(s) 200, 282
AgsI TTSAA 1 cut(s) 104
AjnI CCWGG 1 cut(s) 347
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 2 cut(s) 12, 494
AluI AGCT 2 cut(s) 12, 494
Alw26I GTCTC 1 cut(s) 299
AoxI GGCC 2 cut(s) 284, 391
ApeKI GCWGC 3 cut(s) 398, 428, 504
ApoI RAATTY 1 cut(s) 24
AspLEI GCGC 1 cut(s) 509
AsuHPI GGTGA 1 cut(s) 215
AxyI CCTNAGG 1 cut(s) 281
BalI TGGCCA 1 cut(s) 393
BanI GGYRCC 1 cut(s) 120
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BbvI GCAGC 3 cut(s) 385, 415, 516
BciT130I CCWGG 1 cut(s) 349
BciVI GTATCC 2 cut(s) 249, 491
BcoDI GTCTC 1 cut(s) 299
BfoI RGCGCY 1 cut(s) 510
BfuI GTATCC 2 cut(s) 249, 491
BisI GCNGC 4 cut(s) 399, 429, 505, 519
BlsI GCNGC 4 cut(s) 400, 430, 506, 520
Bme1390I CCNGG 1 cut(s) 349
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 179
Bsa29I ATCGAT 1 cut(s) 487
BsaAI YACGTR 1 cut(s) 525
BsaJI CCNNGG 2 cut(s) 249, 348
BsaWI WCCGGW 1 cut(s) 111
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 282
Bse1I ACTGG 3 cut(s) 134, 333, 374
Bse21I CCTNAGG 1 cut(s) 281
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 349
BseCI ATCGAT 1 cut(s) 487
BseDI CCNNGG 2 cut(s) 249, 348
BseLI CCNNNNNNNGG 2 cut(s) 200, 282
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 3 cut(s) 134, 333, 374
BseXI GCAGC 3 cut(s) 385, 415, 516
BseYI CCCAGC 1 cut(s) 55
BshFI GGCC 2 cut(s) 286, 393
BshNI GGYRCC 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 487
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 2 cut(s) 200, 282
BsmAI GTCTC 1 cut(s) 299
BsmFI GGGAC 1 cut(s) 454
BsnI GGCC 2 cut(s) 286, 393
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 2 cut(s) 484, 488
BspACI CCGC 2 cut(s) 516, 519
BspANI GGCC 2 cut(s) 286, 393
BspCNI CTCAG 1 cut(s) 273
BspDI ATCGAT 1 cut(s) 487
BspLI GGNNCC 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 3 cut(s) 134, 333, 374
BssECI CCNNGG 2 cut(s) 249, 348
BssMI GATC 2 cut(s) 484, 488
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 349
Bst4CI ACNGT 4 cut(s) 79, 154, 361, 478
BstAUI TGTACA 1 cut(s) 267
BstBAI YACGTR 1 cut(s) 525
BstC8I GCNNGC 2 cut(s) 14, 511
BstDEI CTNAG 1 cut(s) 281
BstENI CCTNNNNNAGG 1 cut(s) 280
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 2 cut(s) 487, 491
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 2 cut(s) 484, 488
BstMWI GCNNNNNNNGC 3 cut(s) 56, 510, 515
BstNI CCWGG 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 347
BstSNI TACGTA 1 cut(s) 525
BstV1I GCAGC 3 cut(s) 385, 415, 516
Bsu15I ATCGAT 1 cut(s) 487
Bsu36I CCTNAGG 1 cut(s) 281
BsuI GTATCC 2 cut(s) 249, 491
BsuRI GGCC 2 cut(s) 286, 393
BsuTUI ATCGAT 1 cut(s) 487
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 14, 511
CfoI GCGC 1 cut(s) 509
ClaI ATCGAT 1 cut(s) 487
Csp6I GTAC 2 cut(s) 268, 421
CviAII CATG 3 cut(s) 186, 302, 424
CviJI RGCY 7 cut(s) 12, 286, 347, 393, 413, 428, 494
CviKI_1 RGCY 7 cut(s) 12, 286, 347, 393, 413, 428, 494
CviQI GTAC 2 cut(s) 268, 421
DdeI CTNAG 1 cut(s) 281
DpnI GATC 2 cut(s) 486, 490
DpnII GATC 2 cut(s) 484, 488
EaeI YGGCCR 1 cut(s) 391
Eco105I TACGTA 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 249
Eco81I CCTNAGG 1 cut(s) 281
EcoNI CCTNNNNNAGG 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 347
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 3 cut(s) 189, 305, 427
FaqI GGGAC 1 cut(s) 454
FatI CATG 3 cut(s) 185, 301, 423
FauNDI CATATG 1 cut(s) 289
Fnu4HI GCNGC 4 cut(s) 399, 429, 505, 519
Fsp4HI GCNGC 4 cut(s) 399, 429, 505, 519
GlaI GCGC 1 cut(s) 508
GluI GCNGC 4 cut(s) 399, 429, 505, 519
GsaI CCCAGC 1 cut(s) 59
GsuI CTGGAG 1 cut(s) 350
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 2 cut(s) 286, 393
HapII CCGG 1 cut(s) 112
HhaI GCGC 1 cut(s) 509
Hin1II CATG 3 cut(s) 189, 305, 427
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 1 cut(s) 467
HpaII CCGG 1 cut(s) 112
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 1 cut(s) 268
Hpy188III TCNNGA 2 cut(s) 158, 280
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 4 cut(s) 200, 205, 286, 425
HpyCH4III ACNGT 4 cut(s) 79, 154, 361, 478
HpyCH4IV ACGT 1 cut(s) 524
HpyCH4V TGCA 1 cut(s) 50
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 510, 515
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 1 cut(s) 524
Hsp92II CATG 3 cut(s) 189, 305, 427
HspAI GCGC 1 cut(s) 507
Kzo9I GATC 2 cut(s) 484, 488
Lsp1109I GCAGC 3 cut(s) 385, 415, 516
MaeII ACGT 1 cut(s) 524
MaeIII GTNAC 1 cut(s) 324
MalI GATC 2 cut(s) 486, 490
MboI GATC 2 cut(s) 484, 488
MboII GAAGA 2 cut(s) 97, 116
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 1 cut(s) 24
MluNI TGGCCA 1 cut(s) 393
MlyI GAGTC 1 cut(s) 476
MnlI CCTC 3 cut(s) 156, 276, 416
Mox20I TGGCCA 1 cut(s) 393
Mph1103I ATGCAT 1 cut(s) 52
MscI TGGCCA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 64
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 1 cut(s) 349
MvaI CCWGG 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 56, 510, 515
NdeI CATATG 1 cut(s) 289
NdeII GATC 2 cut(s) 484, 488
NlaIII CATG 3 cut(s) 189, 305, 427
NlaIV GGNNCC 1 cut(s) 122
NsiI ATGCAT 1 cut(s) 52
PkrI GCNGC 4 cut(s) 400, 430, 506, 520
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
Ppu21I YACGTR 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 347
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 1 cut(s) 347
PspN4I GGNNCC 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 2 cut(s) 269, 422
RsaNI GTAC 2 cut(s) 268, 421
RseI CAYNNNNRTG 1 cut(s) 64
SatI GCNGC 4 cut(s) 399, 429, 505, 519
Sau3AI GATC 2 cut(s) 484, 488
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 1 cut(s) 349
SetI ASST 6 cut(s) 14, 216, 234, 278, 496, 527
SmiMI CAYNNNNRTG 1 cut(s) 64
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SnaBI TACGTA 1 cut(s) 525
Sse9I AATT 1 cut(s) 24
SsiI CCGC 2 cut(s) 516, 519
StyD4I CCNGG 1 cut(s) 347
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 4 cut(s) 79, 154, 361, 478
TaiI ACGT 1 cut(s) 527
TaqI TCGA 2 cut(s) 480, 487
TasI AATT 1 cut(s) 24
TatI WGTACW 1 cut(s) 267
TauI GCSGC 1 cut(s) 521
TscAI CASTG 1 cut(s) 84
TseI GCWGC 3 cut(s) 398, 428, 504
TspDTI ATGAA 1 cut(s) 169
TspRI CASTG 1 cut(s) 84
XagI CCTNNNNNAGG 1 cut(s) 280
XapI RAATTY 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.