pycom14g07030

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009068
Physical Location & Seq
Forward (+)
6988892 .. 6989787
896 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
ATGGATACTCGTAAAAGAGAGCGGCTTGAAGGTTTTGGCTTTCACTCTAAAGGCACCTTCAAGAAGAACAAGCAAGCGGGAGAAGAGGGAAAGTATCATAGGGGGGAGATCCAATTGGGTGTTGAGGGTGGTGGGAACTTGACCCAATTGTCCAGCATGATGGGACTCAGTGAGAGAAATGATGAGAATGAGGATGAGAAGATCAGTGAGGTCGAAGATCGCCTTAATTCGATTCAGGAGAGGATCATGAGTTGGGAGGCTGATCAGTCTATGATATGGGATTCGAGCCCGAATGAGTCTCTGGAGTATATGAAGGCTGTGGAGGAGGCTCGGAGATTGATCGAAAGATTGGAAAGATCGTGTTTGGATAAACATGATGAAGAATGTGAGGTGTTACATAGAGCTCACGATGTTCTTCAAACGGCAATGGCTAGGCTGGAGGAAGAGTTCAAGTACATGCTTGTTCAGAACAGGCAACCCTTTGAACCCGAGCACATGTCTTTTCGTTCGAGTGAGGATGATATTGCGGATGGAAGCTCGATAAACTCTTTTGGGGATGATTCGATTGAGGACTCACTTCAAAGGGATAGTGTCAGCAGAGCGTCGGAGGAAGTCATCATTGATTTGGTTCATCCGGATATAGTTCCTGAGCTCAGAGGCATTGCAAATTTGATGTTCAATTGCAATTATGATCAGGAGCCGTCACTCAAAAACAACCAGAACCCGCCGTCCCCACTCCTACGCCAAGCTCAGATTTGCAGAGACTGGAAACTGGAAAAATTGGGAGATTTGCAGAGATTGGAAAAAATGAGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0046903 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

273

Amino Acids

31.52

Weight (kDa)

4.8

Isoelectric Point (pI)

67.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_N PF20669 75 - 156 4.3e-15 Exocyst complex component Exo70 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 148
AccB1I GGYRCC 1 cut(s) 53
AccBSI CCGCTC 1 cut(s) 22
AccIII TCCGGA 1 cut(s) 634
AciI CCGC 4 cut(s) 22, 77, 527, 725
AclWI GGATC 2 cut(s) 103, 251
AcsI RAATTY 1 cut(s) 667
AfaI GTAC 1 cut(s) 455
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 7 cut(s) 29, 61, 419, 451, 485, 581, 679
AloI GAACNNNNNNTCC 4 cut(s) 431, 463, 713, 745
AluBI AGCT 4 cut(s) 404, 537, 652, 749
AluI AGCT 4 cut(s) 404, 537, 652, 749
Alw21I GWGCWC 3 cut(s) 406, 495, 654
Alw26I GTCTC 2 cut(s) 303, 756
AlwI GGATC 2 cut(s) 103, 251
AlwNI CAGNNNCTG 1 cut(s) 765
Ama87I CYCGRG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 634
ApoI RAATTY 1 cut(s) 667
AvaI CYCGRG 1 cut(s) 488
BanI GGYRCC 1 cut(s) 53
BanII GRGCYC 3 cut(s) 290, 406, 654
Bbv12I GWGCWC 3 cut(s) 406, 495, 654
BccI CCATC 2 cut(s) 154, 524
BceAI ACGGC 3 cut(s) 438, 685, 712
BclI TGATCA 2 cut(s) 262, 691
BcoDI GTCTC 2 cut(s) 303, 756
BfaI CTAG 1 cut(s) 432
BisI GCNGC 1 cut(s) 23
BlsI GCNGC 1 cut(s) 24
BmeT110I CYCGRG 1 cut(s) 488
BmiI GGNNCC 2 cut(s) 55, 699
BpmI CTGGAG 2 cut(s) 323, 458
Bpu10I CCTNAGC 1 cut(s) 648
BsaWI WCCGGW 1 cut(s) 634
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bse1I ACTGG 2 cut(s) 770, 777
Bse3DI GCAATG 2 cut(s) 432, 660
BseAI TCCGGA 1 cut(s) 634
BseGI GGATG 5 cut(s) 199, 523, 535, 562, 631
BseMI GCAATG 2 cut(s) 432, 660
BseMII CTCAG 4 cut(s) 181, 639, 667, 764
BseNI ACTGG 2 cut(s) 770, 777
BseRI GAGGAG 1 cut(s) 338
BshNI GGYRCC 1 cut(s) 53
BsiHKAI GWGCWC 3 cut(s) 406, 495, 654
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 1 cut(s) 635
BslFI GGGAC 2 cut(s) 177, 715
BsmAI GTCTC 2 cut(s) 303, 756
BsmFI GGGAC 2 cut(s) 177, 715
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 4 cut(s) 290, 406, 495, 654
Bsp13I TCCGGA 1 cut(s) 634
Bsp143I GATC 8 cut(s) 108, 201, 217, 243, 262, 339, 356, 691
BspACI CCGC 4 cut(s) 22, 77, 527, 725
BspCNI CTCAG 4 cut(s) 180, 640, 666, 763
BspEI TCCGGA 1 cut(s) 634
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 55, 699
BspPI GGATC 2 cut(s) 103, 251
BspT107I GGYRCC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 22
BsrDI GCAATG 2 cut(s) 432, 660
BsrI ACTGG 2 cut(s) 770, 777
BssMI GATC 8 cut(s) 108, 201, 217, 243, 262, 339, 356, 691
Bst6I CTCTTC 2 cut(s) 78, 438
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 4 cut(s) 167, 648, 653, 750
BstF5I GGATG 5 cut(s) 199, 523, 535, 562, 631
BstKTI GATC 8 cut(s) 111, 204, 220, 246, 265, 342, 359, 694
BstMAI GTCTC 2 cut(s) 303, 756
BstMBI GATC 8 cut(s) 108, 201, 217, 243, 262, 339, 356, 691
BstNSI RCATGY 2 cut(s) 460, 499
BstX2I RGATCY 1 cut(s) 108
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 1 cut(s) 108
BtsCI GGATG 5 cut(s) 199, 523, 535, 562, 631
BtsIMutI CAGTG 2 cut(s) 175, 211
Cac8I GCNNGC 1 cut(s) 75
CaiI CAGNNNCTG 1 cut(s) 765
CciI TCATGA 1 cut(s) 246
CseI GACGC 1 cut(s) 591
Csp6I GTAC 1 cut(s) 454
CviAII CATG 5 cut(s) 157, 247, 374, 457, 496
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 4 cut(s) 167, 648, 653, 750
DpnI GATC 8 cut(s) 110, 203, 219, 245, 264, 341, 358, 693
DpnII GATC 8 cut(s) 108, 201, 217, 243, 262, 339, 356, 691
DrdI GACNNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 148
Eam1104I CTCTTC 2 cut(s) 78, 438
EarI CTCTTC 2 cut(s) 78, 438
Ecl136II GAGCTC 2 cut(s) 404, 652
Eco24I GRGCYC 3 cut(s) 290, 406, 654
Eco53kI GAGCTC 2 cut(s) 404, 652
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 2 cut(s) 404, 652
EcoT38I GRGCYC 3 cut(s) 290, 406, 654
FaeI CATG 5 cut(s) 160, 250, 377, 460, 499
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FaqI GGGAC 2 cut(s) 177, 715
FatI CATG 5 cut(s) 156, 246, 373, 456, 495
FauI CCCGC 2 cut(s) 70, 732
FbaI TGATCA 2 cut(s) 262, 691
Fnu4HI GCNGC 1 cut(s) 23
FokI GGATG 5 cut(s) 206, 530, 542, 569, 618
FriOI GRGCYC 3 cut(s) 290, 406, 654
Fsp4HI GCNGC 1 cut(s) 23
FspBI CTAG 1 cut(s) 432
GluI GCNGC 1 cut(s) 23
GsuI CTGGAG 2 cut(s) 323, 458
HapII CCGG 1 cut(s) 635
HgaI GACGC 1 cut(s) 591
Hin1II CATG 5 cut(s) 160, 250, 377, 460, 499
HinfI GANTC 6 cut(s) 165, 232, 281, 296, 560, 572
HpaII CCGG 1 cut(s) 635
Hpy188I TCNGA 5 cut(s) 333, 468, 607, 656, 753
Hpy188III TCNNGA 8 cut(s) 61, 236, 247, 302, 407, 635, 647, 695
Hpy99I CGWCG 1 cut(s) 607
HpyAV CCTTC 3 cut(s) 23, 67, 307
HpyCH4V TGCA 4 cut(s) 665, 684, 759, 793
HpyF3I CTNAG 4 cut(s) 167, 648, 653, 750
Hsp92II CATG 5 cut(s) 160, 250, 377, 460, 499
Kpn2I TCCGGA 1 cut(s) 634
Ksp22I TGATCA 2 cut(s) 262, 691
Kzo9I GATC 8 cut(s) 108, 201, 217, 243, 262, 339, 356, 691
LmnI GCTCC 1 cut(s) 697
MaeI CTAG 1 cut(s) 432
MaeIII GTNAC 2 cut(s) 393, 702
MalI GATC 8 cut(s) 110, 203, 219, 245, 264, 341, 358, 693
MbiI CCGCTC 1 cut(s) 22
MboI GATC 8 cut(s) 108, 201, 217, 243, 262, 339, 356, 691
MboII GAAGA 7 cut(s) 76, 95, 211, 227, 392, 407, 455
MfeI CAATTG 3 cut(s) 113, 146, 679
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 4 cut(s) 290, 406, 495, 654
MluCI AATT 8 cut(s) 113, 146, 226, 667, 679, 685, 779, 814
MlyI GAGTC 3 cut(s) 159, 305, 566
MmeI TCCRAC 1 cut(s) 585
MroI TCCGGA 1 cut(s) 634
MseI TTAA 1 cut(s) 225
MspI CCGG 1 cut(s) 635
MunI CAATTG 3 cut(s) 113, 146, 679
NdeII GATC 8 cut(s) 108, 201, 217, 243, 262, 339, 356, 691
NlaIII CATG 5 cut(s) 160, 250, 377, 460, 499
NlaIV GGNNCC 2 cut(s) 55, 699
NmuCI GTSAC 1 cut(s) 702
NspI RCATGY 2 cut(s) 460, 499
PagI TCATGA 1 cut(s) 246
PciI ACATGT 1 cut(s) 495
PfeI GAWTC 3 cut(s) 232, 281, 560
PkrI GCNGC 1 cut(s) 24
PleI GAGTC 3 cut(s) 159, 304, 566
PpsI GAGTC 3 cut(s) 159, 304, 566
PscI ACATGT 1 cut(s) 495
Psp124BI GAGCTC 2 cut(s) 406, 654
PspN4I GGNNCC 2 cut(s) 55, 699
PstNI CAGNNNCTG 1 cut(s) 765
PsuI RGATCY 1 cut(s) 108
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SacI GAGCTC 2 cut(s) 406, 654
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 1 cut(s) 23
Sau3AI GATC 8 cut(s) 108, 201, 217, 243, 262, 339, 356, 691
SchI GAGTC 3 cut(s) 159, 305, 566
SduI GDGCHC 4 cut(s) 290, 406, 495, 654
SetI ASST 8 cut(s) 34, 59, 213, 393, 406, 539, 654, 751
Sse9I AATT 8 cut(s) 113, 146, 226, 667, 679, 685, 779, 814
SsiI CCGC 4 cut(s) 22, 77, 527, 725
SspMI CTAG 1 cut(s) 432
SstI GAGCTC 2 cut(s) 406, 654
TaqI TCGA 7 cut(s) 213, 230, 284, 342, 509, 539, 563
TasI AATT 8 cut(s) 113, 146, 226, 667, 679, 685, 779, 814
TatI WGTACW 1 cut(s) 453
TauI GCSGC 1 cut(s) 25
TfiI GAWTC 3 cut(s) 232, 281, 560
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 2 cut(s) 175, 211
TseFI GTSAC 1 cut(s) 702
Tsp45I GTSAC 1 cut(s) 702
TspDTI ATGAA 3 cut(s) 326, 393, 620
TspRI CASTG 2 cut(s) 175, 211
XapI RAATTY 1 cut(s) 667
XceI RCATGY 2 cut(s) 460, 499
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.