pycom14g08270
BHLH Family

Transcription factor bHLH106-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012151
Physical Location & Seq
Reverse (-)
8890852 .. 8892277
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGCAGCCGGGTAATCATCAAAGCCAAGAACTCTACCGATTTCTTGTCCAAAACGGCTTAATCAGCGTGAATAATGATCATCAGTTGTCAGCTATGCAGAGCTTTTGCAGCTCTTCTTCTAATAATTATAGTTATTACCCTTTGGAGTTGTCTGGGGTTAGTACTGAACATGACGCCTCGCCTCAGGATAGAGCCCTTGCTGCTCTCAAGAATCACAAGGAGGCCGAGAAAAGAAGGAGAGAGCGAATCAACTCCCACCTCGATAAGCTTCGAGGCCTTCTTCCTTGCAATTCCAAGACAGACAAAGCCTCCCTGTTGGCCAAGGTGGTCCAGAGAGTGAAAGAGCTGAAACAACAAACGTCAGAGATCGAAGAGCTCGAGACCTTCCCTTCTGAAACTGATGAAATCACTGTACTTTCTTCTAATGATTATACAAACGATGGAAGAATTGTTTTCAAGGCCTCTTTGTGCTGCGAGGACCGCTCCGACCTCCTACCTGACCTAATTGAAATTCTAAAATCCCTCCATTTGAAGACAATCAAAGCTGAAATTGCCACTCTTGGGGGAAGAATTCGCAACGTGTTTATTGTTGCCGCAGACAAAGATCGCTCGAGTGAATCAGTCAACTTCTTGCAGAATGCGTTGAAGTCATTACTTGAACGCTCCAACTCTAGTGAAAGATCGAAAAGAAGACGCGTATTAGACCGCACATTAGTTCTATAA

Protein Analysis

241

Amino Acids

27.17

Weight (kDa)

8.3

Isoelectric Point (pI)

57.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 71 - 117 1.6e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41130 AT3G56770
fragaria_vesca FvH4_3g28400
malus_domestica MD12G1095300.v1.1 MD14G1091000.v1.1
prunus_persica Prupe.7G031500_v2.0.a1
pyrus_communis pycom12g08210 pycom14g08270
rosa_chinensis RchiOBHm_Chr5g0053301
rosa_laevigata RLG00000034769
rosa_multiflora Rmu_co8065512.1_g000001 Rmu_sc0001966.1_g000015
rosa_roxburghii Rroxscaffold_1G00029060
rosa_rugosa Rorug05G0267500
rosa_samantha Rh5AG340100 Rh5BG350800 Rh5CG376900 Rh5DG364700
rosa_wichuraiana Rw5G032110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 483
AccII CGCG 1 cut(s) 696
AciI CCGC 3 cut(s) 481, 594, 706
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 2 cut(s) 510, 570
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 2 cut(s) 163, 414
AfiI CCNNNNNNNGG 1 cut(s) 561
AflIII ACRYGT 2 cut(s) 579, 694
AgsI TTSAA 5 cut(s) 457, 509, 532, 646, 659
AluBI AGCT 7 cut(s) 92, 102, 111, 268, 346, 376, 545
AluI AGCT 7 cut(s) 92, 102, 111, 268, 346, 376, 545
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 374
Ama87I CYCGRG 2 cut(s) 377, 610
AoxI GGCC 4 cut(s) 222, 274, 318, 459
ApeKI GCWGC 4 cut(s) 4, 108, 200, 471
ApoI RAATTY 2 cut(s) 510, 570
AspS9I GGNCC 2 cut(s) 328, 478
AsuC2I CCSGG 1 cut(s) 9
AvaI CYCGRG 2 cut(s) 377, 610
AvaII GGWCC 2 cut(s) 328, 478
AxyI CCTNAGG 1 cut(s) 183
BalI TGGCCA 1 cut(s) 320
BanII GRGCYC 2 cut(s) 196, 378
BbsI GAAGAC 2 cut(s) 539, 697
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 4 cut(s) 16, 120, 187, 458
BccI CCATC 1 cut(s) 434
BceAI ACGGC 1 cut(s) 70
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 1 cut(s) 672
BisI GCNGC 5 cut(s) 5, 109, 201, 472, 594
BlsI GCNGC 5 cut(s) 6, 110, 202, 473, 595
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 9
Bme18I GGWCC 2 cut(s) 328, 478
BmeT110I CYCGRG 2 cut(s) 377, 610
BmgT120I GGNCC 2 cut(s) 328, 478
BmrFI CCNGG 1 cut(s) 9
BpiI GAAGAC 2 cut(s) 539, 697
BplI GAGNNNNNCTC 2 cut(s) 467, 499
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 561
Bse21I CCTNAGG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 197
BseXI GCAGC 4 cut(s) 16, 120, 187, 458
Bsh1236I CGCG 1 cut(s) 696
BshFI GGCC 4 cut(s) 224, 276, 320, 461
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 2 cut(s) 377, 610
BsiSI CCGG 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 561
BsmAI GTCTC 1 cut(s) 374
BsmI GAATGC 1 cut(s) 643
BsnI GGCC 4 cut(s) 224, 276, 320, 461
Bso31I GGTCTC 1 cut(s) 374
BsoBI CYCGRG 2 cut(s) 377, 610
Bsp1286I GDGCHC 2 cut(s) 196, 378
Bsp143I GATC 4 cut(s) 76, 366, 604, 680
BspACI CCGC 3 cut(s) 481, 594, 706
BspANI GGCC 4 cut(s) 224, 276, 320, 461
BspCNI CTCAG 1 cut(s) 196
BspFNI CGCG 1 cut(s) 696
BspQI GCTCTTC 2 cut(s) 118, 366
BspTNI GGTCTC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 483
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 4 cut(s) 76, 366, 604, 680
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 2 cut(s) 118, 366
BstACI GRCGYC 1 cut(s) 174
BstDEI CTNAG 1 cut(s) 183
BstFNI CGCG 1 cut(s) 696
BstKTI GATC 4 cut(s) 79, 369, 607, 683
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 4 cut(s) 76, 366, 604, 680
BstMWI GCNNNNNNNGC 5 cut(s) 63, 108, 200, 480, 551
BstSCI CCNGG 1 cut(s) 7
BstUI CGCG 1 cut(s) 696
BstV1I GCAGC 4 cut(s) 16, 120, 187, 458
BstV2I GAAGAC 2 cut(s) 539, 697
Bsu36I CCTNAGG 1 cut(s) 183
BsuRI GGCC 4 cut(s) 224, 276, 320, 461
BtsIMutI CAGTG 1 cut(s) 408
Cfr13I GGNCC 2 cut(s) 328, 478
CseI GACGC 2 cut(s) 182, 702
Csp6I GTAC 2 cut(s) 162, 413
CviAII CATG 1 cut(s) 170
CviQI GTAC 2 cut(s) 162, 413
DdeI CTNAG 1 cut(s) 183
DpnI GATC 4 cut(s) 78, 368, 606, 682
DpnII GATC 4 cut(s) 76, 366, 604, 680
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 2 cut(s) 118, 366
EarI CTCTTC 2 cut(s) 118, 366
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 321
Eco147I AGGCCT 2 cut(s) 276, 461
Eco24I GRGCYC 2 cut(s) 196, 378
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 2 cut(s) 328, 478
Eco53kI GAGCTC 1 cut(s) 376
Eco81I CCTNAGG 1 cut(s) 183
Eco88I CYCGRG 2 cut(s) 377, 610
EcoICRI GAGCTC 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 570
EcoT14I CCWWGG 1 cut(s) 321
EcoT38I GRGCYC 2 cut(s) 196, 378
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 1 cut(s) 173
FaiI YATR 5 cut(s) 95, 129, 171, 432, 721
FatI CATG 1 cut(s) 169
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 5 cut(s) 5, 109, 201, 472, 594
FriOI GRGCYC 2 cut(s) 196, 378
Fsp4HI GCNGC 5 cut(s) 5, 109, 201, 472, 594
FspBI CTAG 1 cut(s) 672
GluI GCNGC 5 cut(s) 5, 109, 201, 472, 594
HaeIII GGCC 4 cut(s) 224, 276, 320, 461
HapII CCGG 1 cut(s) 8
HgaI GACGC 2 cut(s) 182, 702
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 1 cut(s) 173
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 3 cut(s) 211, 246, 617
HpaII CCGG 1 cut(s) 8
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 3 cut(s) 364, 394, 487
Hpy188III TCNNGA 4 cut(s) 185, 208, 331, 379
Hpy8I GTNNAC 1 cut(s) 625
HpyAV CCTTC 4 cut(s) 228, 287, 394, 399
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4IV ACGT 2 cut(s) 359, 579
HpyCH4V TGCA 5 cut(s) 4, 97, 108, 288, 634
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 108, 200, 480, 551
HpyF3I CTNAG 1 cut(s) 183
HpySE526I ACGT 2 cut(s) 359, 579
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 1 cut(s) 173
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 4 cut(s) 76, 366, 604, 680
LguI GCTCTTC 2 cut(s) 118, 366
LmnI GCTCC 2 cut(s) 488, 668
LpnPI CCDG 6 cut(s) 21, 138, 170, 326, 344, 510
Lsp1109I GCAGC 4 cut(s) 16, 120, 187, 458
MaeI CTAG 1 cut(s) 672
MaeII ACGT 2 cut(s) 359, 579
MalI GATC 4 cut(s) 78, 368, 606, 682
MbiI CCGCTC 1 cut(s) 483
MboI GATC 4 cut(s) 76, 366, 604, 680
MboII GAAGA 9 cut(s) 105, 108, 272, 383, 411, 456, 544, 579, 702
MhlI GDGCHC 2 cut(s) 196, 378
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 7 cut(s) 124, 289, 447, 504, 510, 549, 570
MluI ACGCGT 1 cut(s) 694
MluNI TGGCCA 1 cut(s) 320
MmeI TCCRAC 2 cut(s) 510, 690
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MseI TTAA 1 cut(s) 59
Msp20I TGGCCA 1 cut(s) 320
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 643
MvnI CGCG 1 cut(s) 696
MwoI GCNNNNNNNGC 5 cut(s) 63, 108, 200, 480, 551
NciI CCSGG 1 cut(s) 9
NdeII GATC 4 cut(s) 76, 366, 604, 680
NlaIII CATG 1 cut(s) 173
NmeAIII GCCGAG 1 cut(s) 250
PaeR7I CTCGAG 2 cut(s) 377, 610
PceI AGGCCT 2 cut(s) 276, 461
PciSI GCTCTTC 2 cut(s) 118, 366
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PctI GAATGC 1 cut(s) 643
PfeI GAWTC 3 cut(s) 211, 246, 617
PkrI GCNGC 5 cut(s) 6, 110, 202, 473, 595
Psp124BI GAGCTC 1 cut(s) 378
PspPI GGNCC 2 cut(s) 328, 478
PspXI VCTCGAGB 1 cut(s) 610
RsaI GTAC 2 cut(s) 163, 414
RsaNI GTAC 2 cut(s) 162, 413
SacI GAGCTC 1 cut(s) 378
SapI GCTCTTC 2 cut(s) 118, 366
SaqAI TTAA 1 cut(s) 59
SatI GCNGC 5 cut(s) 5, 109, 201, 472, 594
Sau3AI GATC 4 cut(s) 76, 366, 604, 680
Sau96I GGNCC 2 cut(s) 328, 478
ScaI AGTACT 1 cut(s) 163
ScrFI CCNGG 1 cut(s) 9
SduI GDGCHC 2 cut(s) 196, 378
Sfr274I CTCGAG 2 cut(s) 377, 610
SinI GGWCC 2 cut(s) 328, 478
SlaI CTCGAG 2 cut(s) 377, 610
SmlI CTYRAG 3 cut(s) 206, 377, 610
SmoI CTYRAG 3 cut(s) 206, 377, 610
Sse9I AATT 7 cut(s) 124, 289, 447, 504, 510, 549, 570
SseBI AGGCCT 2 cut(s) 276, 461
SsiI CCGC 3 cut(s) 481, 594, 706
SspMI CTAG 1 cut(s) 672
SstI GAGCTC 1 cut(s) 378
StuI AGGCCT 2 cut(s) 276, 461
StyD4I CCNGG 1 cut(s) 7
StyI CCWWGG 1 cut(s) 321
TaaI ACNGT 1 cut(s) 412
TaiI ACGT 2 cut(s) 362, 582
TaqI TCGA 6 cut(s) 261, 271, 369, 378, 611, 683
TasI AATT 7 cut(s) 124, 289, 447, 504, 510, 549, 570
TatI WGTACW 2 cut(s) 161, 412
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 3 cut(s) 211, 246, 617
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TscAI CASTG 1 cut(s) 415
TseI GCWGC 4 cut(s) 4, 108, 200, 471
TspDTI ATGAA 1 cut(s) 417
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 2 cut(s) 328, 478
XapI RAATTY 2 cut(s) 510, 570
XhoI CTCGAG 2 cut(s) 377, 610
XspI CTAG 1 cut(s) 672
ZrmI AGTACT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.