pycom14g10840

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003275
Physical Location & Seq
Reverse (-)
13625606 .. 13628788
3183 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2169 bp
ATGGGGAGCTATGGTGAAGAGCGTGAGGATCAATTTTATGATACCCGAGAGGACCTTTCTTCGGTTTCGGATTGGGGTTCAGATTGCTGTGAGGAGTTTAGTTCTGGTACTCCAGGTTTCTTAAGGTATGAGGAGGTGTGGGCTAACAACCTGGAAAGTGTTCATGAGCGCCGCCATAAGTTCCTGCAGTGGATGGGTGTAGATTTGGATTCGAATTCGATGACGACAGAAGACTCAGAAGATGTCTTTGTTGACAGAATTAAAATTATTGATAGAATTAGGGAAGATAGTGGGGTGGTGTTGAGAACTTGGGGATTGGAAGAGGCGTCTTGCTCAAGTCAGTCAACGGTGTCTTCTTTGTCAACTGAAGCGCGGGAATCACTAGGAAATGGTGTTTTGGATGATACACATTCCACACGTGCAATGAGGAATTTGAACGGCGGAATGACTGTCATTGTAAGTGAATTGGTTCAGGATGGAAGGCTTGGCAGATTAGGTCAAGCGGGGCTAAGTCAATTGGTTAATGGGGAAGTTCTCAGTGCACAGGCACCATCTCCTTTGATTCAGCAATTATTGCAGAGAGAAGTTGATAACGCTAGGTTCTTTCTTGATGCGAAGAAGAAAGTTAAAAGGGGATGGTTAAGGAAATTAGGAAATGGGATTTGCGTTGCTGATAAACATGAGGCTGCGTTGAGCCCCATCAGTCTTAAATCTGCGTCAGGGGTAGGGATGCAAAGAGTCCAAGTTCATCCAAGTAAAAAGCGGACCAAGGAATTATCTTCACTGTATGCTGGGCAACACTTCTCAGCTCACGAGGGTTCAATTTTGTCCATGAAGTTCAGTCTTGATGGGCAATACTTGGCCAGTGCTGGTGAAGATGGTGTCGTGAGAGTGTGGAAGGTGATTGAGGATGAGAGATTCAACCAATTTGATATCGCTTCTGATCCTTCTTCTCTGTACTTCAAGATGCATCCATTTTCCAAGTTAAACTCTCCAAATGTGAATAAAGAAAAACAGAGTAAGGCGCAGCAATTGGTGGGATCATCAGAGTCAGCTTGTGTCGTTTTCCCTCCAAAGATATTTCGCTTATTGGAGAAGCCTGTTCATGAGTTCTGGGGACACAGAGGGGAGGTCTTGGATCTCTCATGGGCAAAGAAAGGGTTTCTGCTGTCATCCTCTGTTGATAAGACAGTCCGTTTATGGCAAGTAGGATGCGACAGATGCCTAAGAGTCTTTTCTCACAGTAATTTTGTGACATGTGTTAATTTCAACCCTCTGGATGATAATTATTTCATCAGTGGTTCAATAGACGGAAAAGTTCGCATTTGGGAAATTATTCGTTGTCTGGTTATTGACTATATTGATGTTCGTGAGATAGTCACTGCTGTATCTTATCGTCCTGACGGAAAGGGAGGAATTGTGGGTTTGATGACTGGAAATTGCTGTGTCTATAACATCATAGATGACCATCTGGAATTGGATGCTCAGATAAACATACACGGCAAAAAGAAGTCACCTGGAAAAAGGATAACTGGCTTTCAGTTTTCTCCAAGCAACCCAAGCAAAGTTATGGTGACGTCTGCTGATTCAGTAGTCCGTATAATTTCTGGGAAAGATATCGTCTGCAAATATAGGGGTCTTCGAAATGGAGGAAACCATATGTCCGCCTCTTTTACCTCAGACGGAAAGCACATTGTCTCAGCAAGCGATGATTCAAATGTACGCATCTGGAACTTCAATAGCCAGGACACGACATCTTTGCGATCGAGGAACCTATGGTCTTGTGAGAGTTTCGTGTCCCACAACTCCATGATTGCTATACCTTGGAATGGTGTAAACAGCTTGCCGGGAACCCTTCCATCCCCCAGATTCATTGGGGATTTGCCGCGAAGCAGCCTTGATTATCCCCTAAAACATTACAATTTGGATGAGAAAGTAGAACAGAAGTCCTTGCCAAGGGGTACCGCAACTTGGCCCGAGGAGAAACTTGTAAACTCAAGTCCAGTACCCATTTCCCCAACGATGTGCAGATCCGAGTATAAGTTTTTGAAGAGTGCTTGCCATGGTATGTCTGGCTCTTCTCACATGTGGGGCCTTGTGATCGTCACCGCCAGCCTGGACGGACGGATTAGAACATACCACAATTACGGGTTACCTATTCGTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

723

Amino Acids

80.55

Weight (kDa)

7.57

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 245 - 309 5.7e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 260 - 321 8.1e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 264 - 444 2.3e-11 WDR3 second beta-propeller domain
WD40 PF00400 267 - 299 7.2e-08 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 267 - 321 1e-05 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 267 - 319 3.9e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 359 - 585 4e-31 WDR5 beta-propeller domain
WD40_Prp19 PF24814 365 - 486 3.5e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 365 - 445 4e-11 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 366 - 428 6.9e-07 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 366 - 534 2.6e-10 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 371 - 450 2.4e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 374 - 586 3.5e-22 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 381 - 581 1e-10 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 391 - 492 7.5e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 407 - 443 2.7e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 428 - 611 4.3e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 457 - 585 9.4e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 520 - 610 1.2e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1580
Acc65I GGTACC 1 cut(s) 1961
AccB1I GGYRCC 2 cut(s) 547, 1961
AccII CGCG 2 cut(s) 373, 1888
AciI CCGC 9 cut(s) 172, 373, 441, 503, 763, 1665, 1886, 1965, 2109
AclWI GGATC 5 cut(s) 36, 938, 1048, 1146, 2025
AcoI YGGCCR 1 cut(s) 861
AcsI RAATTY 2 cut(s) 214, 430
AcuI CTGAAG 1 cut(s) 387
AcvI CACGTG 1 cut(s) 419
AcyI GRCGYC 2 cut(s) 326, 1577
AfaI GTAC 5 cut(s) 109, 959, 1722, 1963, 2007
AfiI CCNNNNNNNGG 4 cut(s) 61, 1829, 1956, 1971
AflII CTTAAG 1 cut(s) 121
AflIII ACRYGT 3 cut(s) 416, 1256, 2085
AgsI TTSAA 9 cut(s) 436, 822, 922, 964, 1270, 1305, 1716, 1738, 2050
AjnI CCWGG 5 cut(s) 112, 150, 1516, 1743, 2115
AluBI AGCT 4 cut(s) 9, 809, 1055, 1842
AluI AGCT 4 cut(s) 9, 809, 1055, 1842
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 1 cut(s) 1702
Alw44I GTGCAC 1 cut(s) 540
AlwI GGATC 5 cut(s) 36, 938, 1048, 1146, 2025
Ama87I CYCGRG 2 cut(s) 45, 1976
AoxI GGCC 3 cut(s) 861, 1973, 2092
ApaLI GTGCAC 1 cut(s) 540
ApeKI GCWGC 3 cut(s) 686, 1027, 1893
ApoI RAATTY 2 cut(s) 214, 430
Asp700I GAANNNNTTC 3 cut(s) 159, 468, 1335
Asp718I GGTACC 1 cut(s) 1961
AspLEI GCGC 3 cut(s) 171, 373, 1027
AspS9I GGNCC 4 cut(s) 52, 765, 1974, 2092
AsuC2I CCSGG 1 cut(s) 1848
AsuHPI GGTGA 6 cut(s) 26, 884, 913, 1506, 1585, 2098
AsuII TTCGAA 2 cut(s) 212, 1642
AvaI CYCGRG 2 cut(s) 45, 1976
AvaII GGWCC 2 cut(s) 52, 765
BaeGI GKGCMC 1 cut(s) 544
BaeI ACNNNNGTAYC 2 cut(s) 1371, 1404
BalI TGGCCA 1 cut(s) 863
BanI GGYRCC 2 cut(s) 547, 1961
BanII GRGCYC 1 cut(s) 698
BauI CACGAG 1 cut(s) 812
BbrPI CACGTG 1 cut(s) 419
BbsI GAAGAC 3 cut(s) 237, 345, 1631
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 3 cut(s) 673, 1039, 1905
BccI CCATC 9 cut(s) 187, 470, 559, 630, 707, 842, 872, 1476, 1867
BceAI ACGGC 2 cut(s) 454, 1516
BcgI CGANNNNNNTGC 2 cut(s) 1741, 1775
BciT130I CCWGG 5 cut(s) 114, 152, 1518, 1745, 2117
BcnI CCSGG 1 cut(s) 1848
BcoDI GTCTC 1 cut(s) 1702
BfaI CTAG 2 cut(s) 383, 597
BfmI CTRYAG 1 cut(s) 185
BfoI RGCGCY 1 cut(s) 172
BfrI CTTAAG 1 cut(s) 121
BisI GCNGC 5 cut(s) 172, 687, 1028, 1886, 1894
BlsI GCNGC 5 cut(s) 173, 688, 1029, 1887, 1895
Bme1390I CCNGG 6 cut(s) 114, 152, 1518, 1745, 1848, 2117
Bme18I GGWCC 2 cut(s) 52, 765
BmeT110I CYCGRG 2 cut(s) 45, 1976
BmgT120I GGNCC 4 cut(s) 52, 765, 1974, 2092
BmiI GGNNCC 5 cut(s) 549, 1772, 1852, 1963, 2093
BmrFI CCNGG 6 cut(s) 114, 152, 1518, 1745, 1848, 2117
BmsI GCATC 8 cut(s) 601, 720, 957, 979, 1202, 1211, 1471, 1734
BpiI GAAGAC 3 cut(s) 237, 345, 1631
BpmI CTGGAG 1 cut(s) 96
Bpu14I TTCGAA 2 cut(s) 212, 1642
BpuEI CTTGAG 2 cut(s) 319, 1981
BpuMI CCSGG 1 cut(s) 1848
BsaAI YACGTR 1 cut(s) 419
BsaBI GATNNNNATC 1 cut(s) 1467
BsaHI GRCGYC 2 cut(s) 326, 1577
BsaJI CCNNGG 5 cut(s) 768, 1823, 1955, 1977, 2062
BsaXI ACNNNNNCTCC 3 cut(s) 28, 1404, 1434
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 1829, 1956, 1971
Bse1I ACTGG 4 cut(s) 864, 1438, 1537, 2003
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 1467
BseBI CCWGG 5 cut(s) 114, 152, 1518, 1745, 2117
BseDI CCNNGG 5 cut(s) 768, 1823, 1955, 1977, 2062
BseJI GATNNNNATC 1 cut(s) 1467
BseLI CCNNNNNNNGG 4 cut(s) 61, 1829, 1956, 1971
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 6 cut(s) 249, 550, 819, 1499, 1692, 1713
BseNI ACTGG 4 cut(s) 864, 1438, 1537, 2003
BseRI GAGGAG 3 cut(s) 107, 146, 1994
BseSI GKGCMC 1 cut(s) 544
BseXI GCAGC 3 cut(s) 673, 1039, 1905
BseYI CCCAGC 1 cut(s) 791
BsgI GTGCAG 1 cut(s) 2047
Bsh1236I CGCG 2 cut(s) 373, 1888
Bsh1285I CGRYCG 1 cut(s) 1766
BshFI GGCC 3 cut(s) 863, 1975, 2094
BshNI GGYRCC 2 cut(s) 547, 1961
BsiEI CGRYCG 1 cut(s) 1766
BsiHKAI GWGCWC 1 cut(s) 544
BsiHKCI CYCGRG 2 cut(s) 45, 1976
BsiSI CCGG 1 cut(s) 1847
BslFI GGGAC 2 cut(s) 1131, 1783
BslI CCNNNNNNNGG 4 cut(s) 61, 1829, 1956, 1971
BsmAI GTCTC 1 cut(s) 1702
BsmFI GGGAC 2 cut(s) 1131, 1783
BsnI GGCC 3 cut(s) 863, 1975, 2094
BsoBI CYCGRG 2 cut(s) 45, 1976
Bsp119I TTCGAA 2 cut(s) 212, 1642
Bsp1286I GDGCHC 2 cut(s) 544, 698
Bsp143I GATC 7 cut(s) 28, 943, 1040, 1138, 1763, 2030, 2100
Bsp19I CCATGG 1 cut(s) 2062
BspACI CCGC 9 cut(s) 172, 373, 441, 503, 763, 1665, 1886, 1965, 2109
BspANI GGCC 3 cut(s) 863, 1975, 2094
BspCNI CTCAG 6 cut(s) 248, 549, 818, 1498, 1691, 1712
BspFNI CGCG 2 cut(s) 373, 1888
BspHI TCATGA 2 cut(s) 163, 1105
BspLI GGNNCC 5 cut(s) 549, 1772, 1852, 1963, 2093
BspMAI CTGCAG 1 cut(s) 189
BspPI GGATC 5 cut(s) 36, 938, 1048, 1146, 2025
BspQI GCTCTTC 2 cut(s) 12, 2083
BspT104I TTCGAA 2 cut(s) 212, 1642
BspT107I GGYRCC 2 cut(s) 547, 1961
BspTI CTTAAG 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 4 cut(s) 864, 1438, 1537, 2003
BssECI CCNNGG 5 cut(s) 768, 1823, 1955, 1977, 2062
BssMI GATC 7 cut(s) 28, 943, 1040, 1138, 1763, 2030, 2100
BssNI GRCGYC 2 cut(s) 326, 1577
BssSI CACGAG 1 cut(s) 812
BssT1I CCWWGG 4 cut(s) 768, 1823, 1955, 2062
Bst2BI CACGAG 1 cut(s) 812
Bst2UI CCWGG 5 cut(s) 114, 152, 1518, 1745, 2117
Bst4CI ACNGT 5 cut(s) 349, 451, 786, 1192, 1244
Bst6I CTCTTC 4 cut(s) 12, 315, 2045, 2083
BstACI GRCGYC 2 cut(s) 326, 1577
BstAFI CTTAAG 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 574
BstBAI YACGTR 1 cut(s) 419
BstBI TTCGAA 2 cut(s) 212, 1642
BstC8I GCNNGC 4 cut(s) 1705, 1844, 2059, 2113
BstDEI CTNAG 8 cut(s) 235, 509, 536, 805, 1226, 1485, 1678, 1699
BstDSI CCRYGG 1 cut(s) 2062
BstEII GGTNACC 1 cut(s) 2151
BstENI CCTNNNNNAGG 1 cut(s) 1954
BstFNI CGCG 2 cut(s) 373, 1888
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 3 cut(s) 171, 373, 1027
BstKTI GATC 7 cut(s) 31, 946, 1043, 1141, 1766, 2033, 2103
BstMAI GTCTC 1 cut(s) 1702
BstMBI GATC 7 cut(s) 28, 943, 1040, 1138, 1763, 2030, 2100
BstMCI CGRYCG 1 cut(s) 1766
BstMWI GCNNNNNNNGC 3 cut(s) 574, 1221, 1560
BstNI CCWGG 5 cut(s) 114, 152, 1518, 1745, 2117
BstNSI RCATGY 2 cut(s) 1260, 2089
BstPI GGTNACC 1 cut(s) 2151
BstSCI CCNGG 6 cut(s) 112, 150, 1516, 1743, 1846, 2115
BstSFI CTRYAG 1 cut(s) 185
BstSLI GKGCMC 1 cut(s) 544
BstUI CGCG 2 cut(s) 373, 1888
BstV1I GCAGC 3 cut(s) 673, 1039, 1905
BstV2I GAAGAC 3 cut(s) 237, 345, 1631
BstX2I RGATCY 2 cut(s) 1138, 2030
BstYI RGATCY 2 cut(s) 1138, 2030
BsuRI GGCC 3 cut(s) 863, 1975, 2094
BtgI CCRYGG 1 cut(s) 2062
BtgZI GCGATG 1 cut(s) 1722
BtsI GCAGTG 2 cut(s) 194, 1380
BtsIMutI CAGTG 6 cut(s) 194, 544, 782, 871, 1303, 1380
Cac8I GCNNGC 4 cut(s) 1705, 1844, 2059, 2113
CciI TCATGA 2 cut(s) 163, 1105
CfoI GCGC 3 cut(s) 171, 373, 1027
Cfr13I GGNCC 4 cut(s) 52, 765, 1974, 2092
CseI GACGC 2 cut(s) 315, 705
Csp6I GTAC 5 cut(s) 108, 958, 1721, 1962, 2006
CviAII CATG 9 cut(s) 164, 680, 832, 1106, 1146, 1257, 1810, 2063, 2086
CviQI GTAC 5 cut(s) 108, 958, 1721, 1962, 2006
DdeI CTNAG 8 cut(s) 235, 509, 536, 805, 1226, 1485, 1678, 1699
DpnI GATC 7 cut(s) 30, 945, 1042, 1140, 1765, 2032, 2102
DpnII GATC 7 cut(s) 28, 943, 1040, 1138, 1763, 2030, 2100
EaeI YGGCCR 1 cut(s) 861
Eam1104I CTCTTC 4 cut(s) 12, 315, 2045, 2083
EarI CTCTTC 4 cut(s) 12, 315, 2045, 2083
EciI GGCGGA 2 cut(s) 456, 1654
Eco130I CCWWGG 4 cut(s) 768, 1823, 1955, 2062
Eco24I GRGCYC 1 cut(s) 698
Eco32I GATATC 2 cut(s) 934, 1618
Eco47I GGWCC 2 cut(s) 52, 765
Eco57I CTGAAG 1 cut(s) 387
Eco72I CACGTG 1 cut(s) 419
Eco88I CYCGRG 2 cut(s) 45, 1976
Eco91I GGTNACC 1 cut(s) 2151
EcoNI CCTNNNNNAGG 1 cut(s) 1954
EcoO109I RGGNCCY 2 cut(s) 52, 2092
EcoO65I GGTNACC 1 cut(s) 2151
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 5 cut(s) 112, 150, 1516, 1743, 2115
EcoRV GATATC 2 cut(s) 934, 1618
EcoT14I CCWWGG 4 cut(s) 768, 1823, 1955, 2062
EcoT22I ATGCAT 1 cut(s) 972
EcoT38I GRGCYC 1 cut(s) 698
ErhI CCWWGG 4 cut(s) 768, 1823, 1955, 2062
FaeI CATG 9 cut(s) 167, 683, 835, 1109, 1149, 1260, 1813, 2066, 2089
FaqI GGGAC 2 cut(s) 1131, 1783
FatI CATG 9 cut(s) 163, 679, 831, 1105, 1145, 1256, 1809, 2062, 2085
FauI CCCGC 2 cut(s) 366, 496
FauNDI CATATG 1 cut(s) 1659
Fnu4HI GCNGC 5 cut(s) 172, 687, 1028, 1886, 1894
FriOI GRGCYC 1 cut(s) 698
Fsp4HI GCNGC 5 cut(s) 172, 687, 1028, 1886, 1894
FspBI CTAG 2 cut(s) 383, 597
GlaI GCGC 3 cut(s) 170, 372, 1026
GluI GCNGC 5 cut(s) 172, 687, 1028, 1886, 1894
GsaI CCCAGC 1 cut(s) 795
GsuI CTGGAG 1 cut(s) 96
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 3 cut(s) 863, 1975, 2094
HapII CCGG 1 cut(s) 1847
HgaI GACGC 2 cut(s) 315, 705
HhaI GCGC 3 cut(s) 171, 373, 1027
Hin1I GRCGYC 2 cut(s) 326, 1577
Hin1II CATG 9 cut(s) 167, 683, 835, 1109, 1149, 1260, 1813, 2066, 2089
Hin6I GCGC 3 cut(s) 169, 371, 1025
HinP1I GCGC 3 cut(s) 169, 371, 1025
HincII GTYRAC 3 cut(s) 253, 345, 363
HindII GTYRAC 3 cut(s) 253, 345, 363
HpaII CCGG 1 cut(s) 1847
HphI GGTGA 6 cut(s) 26, 884, 913, 1506, 1585, 2098
Hpy166II GTNNAC 6 cut(s) 253, 345, 363, 542, 1837, 1993
Hpy188I TCNGA 8 cut(s) 70, 82, 238, 943, 1048, 1488, 1681, 2035
Hpy8I GTNNAC 6 cut(s) 253, 345, 363, 542, 1837, 1993
Hpy99I CGWCG 1 cut(s) 2166
HpyAV CCTTC 4 cut(s) 474, 892, 957, 1865
HpyCH4III ACNGT 5 cut(s) 349, 451, 786, 1192, 1244
HpyCH4IV ACGT 2 cut(s) 418, 1577
HpyCH4V TGCA 8 cut(s) 187, 422, 542, 577, 733, 970, 1626, 2028
HpyF10VI GCNNNNNNNGC 3 cut(s) 574, 1221, 1560
HpyF3I CTNAG 8 cut(s) 235, 509, 536, 805, 1226, 1485, 1678, 1699
HpySE526I ACGT 2 cut(s) 418, 1577
Hsp92I GRCGYC 2 cut(s) 326, 1577
Hsp92II CATG 9 cut(s) 167, 683, 835, 1109, 1149, 1260, 1813, 2066, 2089
HspAI GCGC 3 cut(s) 169, 371, 1025
KpnI GGTACC 1 cut(s) 1965
Kzo9I GATC 7 cut(s) 28, 943, 1040, 1138, 1763, 2030, 2100
LguI GCTCTTC 2 cut(s) 12, 2083
LmnI GCTCC 1 cut(s) 6
Lsp1109I GCAGC 3 cut(s) 673, 1039, 1905
LweI GCATC 8 cut(s) 601, 720, 957, 979, 1202, 1211, 1471, 1734
MaeI CTAG 2 cut(s) 383, 597
MaeII ACGT 2 cut(s) 418, 1577
MaeIII GTNAC 6 cut(s) 1252, 1378, 1512, 1573, 2104, 2151
MalI GATC 7 cut(s) 30, 945, 1042, 1140, 1765, 2032, 2102
MboI GATC 7 cut(s) 28, 943, 1040, 1138, 1763, 2030, 2100
MfeI CAATTG 2 cut(s) 515, 1031
MflI RGATCY 2 cut(s) 1138, 2030
MhlI GDGCHC 2 cut(s) 544, 698
MlsI TGGCCA 1 cut(s) 863
MluNI TGGCCA 1 cut(s) 863
MlyI GAGTC 4 cut(s) 227, 747, 1058, 1239
Mox20I TGGCCA 1 cut(s) 863
Mph1103I ATGCAT 1 cut(s) 972
MroXI GAANNNNTTC 3 cut(s) 159, 468, 1335
MscI TGGCCA 1 cut(s) 863
MseI TTAA 9 cut(s) 122, 261, 522, 627, 641, 708, 986, 1263, 2167
Msp20I TGGCCA 1 cut(s) 863
MspCI CTTAAG 1 cut(s) 121
MspI CCGG 1 cut(s) 1847
MspR9I CCNGG 6 cut(s) 114, 152, 1518, 1745, 1848, 2117
MunI CAATTG 2 cut(s) 515, 1031
MvaI CCWGG 5 cut(s) 114, 152, 1518, 1745, 2117
MvnI CGCG 2 cut(s) 373, 1888
MwoI GCNNNNNNNGC 3 cut(s) 574, 1221, 1560
NciI CCSGG 1 cut(s) 1848
NcoI CCATGG 1 cut(s) 2062
NdeI CATATG 1 cut(s) 1659
NdeII GATC 7 cut(s) 28, 943, 1040, 1138, 1763, 2030, 2100
NlaIII CATG 9 cut(s) 167, 683, 835, 1109, 1149, 1260, 1813, 2066, 2089
NlaIV GGNNCC 5 cut(s) 549, 1772, 1852, 1963, 2093
NmuCI GTSAC 5 cut(s) 1252, 1378, 1512, 1573, 2104
NsiI ATGCAT 1 cut(s) 972
NspI RCATGY 2 cut(s) 1260, 2089
NspV TTCGAA 2 cut(s) 212, 1642
PagI TCATGA 2 cut(s) 163, 1105
PciI ACATGT 2 cut(s) 1256, 2085
PciSI GCTCTTC 2 cut(s) 12, 2083
PdmI GAANNNNTTC 3 cut(s) 159, 468, 1335
PfeI GAWTC 7 cut(s) 209, 377, 562, 918, 1586, 1712, 1869
PkrI GCNGC 5 cut(s) 173, 688, 1029, 1887, 1895
Ple19I CGATCG 1 cut(s) 1766
PleI GAGTC 4 cut(s) 227, 746, 1057, 1238
PmaCI CACGTG 1 cut(s) 419
PmlI CACGTG 1 cut(s) 419
PpsI GAGTC 4 cut(s) 227, 746, 1057, 1238
Ppu21I YACGTR 1 cut(s) 419
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 2 cut(s) 1256, 2085
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 5 cut(s) 112, 150, 1516, 1743, 2115
PspCI CACGTG 1 cut(s) 419
PspEI GGTNACC 1 cut(s) 2151
PspFI CCCAGC 1 cut(s) 791
PspGI CCWGG 5 cut(s) 112, 150, 1516, 1743, 2115
PspN4I GGNNCC 5 cut(s) 549, 1772, 1852, 1963, 2093
PspPI GGNCC 4 cut(s) 52, 765, 1974, 2092
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 189
PsuI RGATCY 2 cut(s) 1138, 2030
PvuI CGATCG 1 cut(s) 1766
RsaI GTAC 5 cut(s) 109, 959, 1722, 1963, 2007
RsaNI GTAC 5 cut(s) 108, 958, 1721, 1962, 2006
SapI GCTCTTC 2 cut(s) 12, 2083
SaqAI TTAA 9 cut(s) 122, 261, 522, 627, 641, 708, 986, 1263, 2167
SatI GCNGC 5 cut(s) 172, 687, 1028, 1886, 1894
Sau3AI GATC 7 cut(s) 28, 943, 1040, 1138, 1763, 2030, 2100
Sau96I GGNCC 4 cut(s) 52, 765, 1974, 2092
SchI GAGTC 4 cut(s) 227, 747, 1058, 1239
ScrFI CCNGG 6 cut(s) 114, 152, 1518, 1745, 1848, 2117
SduI GDGCHC 2 cut(s) 544, 698
SfaNI GCATC 8 cut(s) 601, 720, 957, 979, 1202, 1211, 1471, 1734
SfcI CTRYAG 1 cut(s) 185
SfuI TTCGAA 2 cut(s) 212, 1642
SinI GGWCC 2 cut(s) 52, 765
SmlI CTYRAG 3 cut(s) 121, 334, 1996
SmoI CTYRAG 3 cut(s) 121, 334, 1996
SsiI CCGC 9 cut(s) 172, 373, 441, 503, 763, 1665, 1886, 1965, 2109
SspMI CTAG 2 cut(s) 383, 597
StyD4I CCNGG 6 cut(s) 112, 150, 1516, 1743, 1846, 2115
StyI CCWWGG 4 cut(s) 768, 1823, 1955, 2062
TaaI ACNGT 5 cut(s) 349, 451, 786, 1192, 1244
TaiI ACGT 2 cut(s) 421, 1580
TaqI TCGA 4 cut(s) 212, 218, 1642, 1766
TatI WGTACW 1 cut(s) 957
TauI GCSGC 2 cut(s) 174, 1888
TfiI GAWTC 7 cut(s) 209, 377, 562, 918, 1586, 1712, 1869
Tru1I TTAA 9 cut(s) 122, 261, 522, 627, 641, 708, 986, 1263, 2167
Tru9I TTAA 9 cut(s) 122, 261, 522, 627, 641, 708, 986, 1263, 2167
TscAI CASTG 6 cut(s) 194, 544, 789, 871, 1303, 1387
TseFI GTSAC 5 cut(s) 1252, 1378, 1512, 1573, 2104
TseI GCWGC 3 cut(s) 686, 1027, 1893
Tsp45I GTSAC 5 cut(s) 1252, 1378, 1512, 1573, 2104
TspDTI ATGAA 6 cut(s) 152, 737, 848, 1094, 1282, 1861
TspGWI ACGGA 7 cut(s) 1184, 1326, 1419, 1586, 1698, 2136, 2140
TspRI CASTG 6 cut(s) 194, 544, 789, 871, 1303, 1387
Vha464I CTTAAG 1 cut(s) 121
VneI GTGCAC 1 cut(s) 540
VpaK11BI GGWCC 2 cut(s) 52, 765
XagI CCTNNNNNAGG 1 cut(s) 1954
XapI RAATTY 2 cut(s) 214, 430
XceI RCATGY 2 cut(s) 1260, 2089
XcmI CCANNNNNNNNNTGG 1 cut(s) 2069
XmnI GAANNNNTTC 3 cut(s) 159, 468, 1335
XspI CTAG 2 cut(s) 383, 597
ZraI GACGTC 1 cut(s) 1578
Zsp2I ATGCAT 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.