pycom14g11490

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016592
Physical Location & Seq
Forward (+)
14591961 .. 14592912
952 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g11490.3

Sequence Viewer

Length: 786 bp
ATGTTGGTGAAGATAATCAGCCAAGGCACAATAAAACTGAATATAGCTCTTTGTTGGTCACAGCCAGATATAGCCGCGCCTGGTGTAGGTATATTAGCCACCAGTTCTTATGATCCCGCAATGGATGGAGGATTTGCCTTGCTATCGGGGACATCAATGGCAACTCCCCACATTGCAGGCCTTGTGGCACTTCTAAAATCGTTGCAGCCTGATTGGTCCCCTGCTGCAATGAAATCAGCACTAGTCACAACAGCATGGAAGACTGATCCATTTGGAGAGCCAATCTTTGCTGAAGGGGCAGCGCATAAACTTGCAGATCTATTTGATTATGGAGGAGGATTAGTGAACCCGAACAAGGCAGCAAACCCTGGCCTTATATACATTATGGACACCAATGACTACATAAACTACCTTTGTGCGTTTGGCTACAATACCTCAGCTATTTCTCTACTTGTTAAACAAGCCACATCATGTCCTGTTCCAAAGCCTTCAATTCTTGATGTGAACCTGCCTTCAATTACCATTCCAAATCTAAGTAAGAAATCCTTTAACACCCACTCGAAGTGTAACAAACGTTGGTCCTGGCAAGTACTCGTCAGGCCAGAGACATTGGTCTTCAACTCTACAGTTAAGACAATTTCCTTTTCAGTTGCGGTTTCCACCACCTACCGAGTGAATACAACATACTTTTTCGGAAGCCTGACATGGACTGATGGGGTGCATGCTGTGACAAGTCCCACATCTGTTAGAACACAAATCATACGATCCTATACTGATGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

262

Amino Acids

28.12

Weight (kDa)

8.6

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016592)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32980
malus_domestica MD14G1139200.v1.1
pyrus_communis pycom14g11490
rosa_chinensis RchiOBHm_Chr5g0002201
rosa_multiflora Rmu_sc0001492.1_g000003
rosa_roxburghii Rroxscaffold_3G00267180
rosa_rugosa Rorug06G0483500
rosa_samantha Rh5AG017200 Rh7AG088600 Rh7BG090000 Rh7BG090400 Rh7CG089200 Rh7CG089400 Rh7DG090800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 516
AccII CGCG 1 cut(s) 77
AciI CCGC 3 cut(s) 75, 117, 653
AclI AACGTT 1 cut(s) 574
AclWI GGATC 3 cut(s) 107, 260, 759
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 1 cut(s) 591
AfiI CCNNNNNNNGG 2 cut(s) 86, 355
AgsI TTSAA 3 cut(s) 492, 516, 619
AhlI ACTAGT 1 cut(s) 241
AjnI CCWGG 3 cut(s) 79, 367, 581
AluBI AGCT 2 cut(s) 47, 440
AluI AGCT 2 cut(s) 47, 440
Alw26I GTCTC 1 cut(s) 599
AlwI GGATC 3 cut(s) 107, 260, 759
AoxI GGCC 3 cut(s) 178, 370, 599
ApeKI GCWGC 4 cut(s) 205, 224, 299, 359
AspLEI GCGC 2 cut(s) 79, 304
AspS9I GGNCC 2 cut(s) 216, 579
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 2 cut(s) 216, 579
BbsI GAAGAC 2 cut(s) 266, 607
BbvCI CCTCAGC 1 cut(s) 436
BbvI GCAGC 4 cut(s) 211, 217, 311, 371
BccI CCATC 2 cut(s) 119, 707
BciT130I CCWGG 3 cut(s) 81, 369, 583
BcoDI GTCTC 1 cut(s) 599
BcuI ACTAGT 1 cut(s) 241
BfaI CTAG 1 cut(s) 242
BfmI CTRYAG 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 516
BglII AGATCT 1 cut(s) 316
BisI GCNGC 5 cut(s) 75, 206, 225, 300, 360
BlsI GCNGC 5 cut(s) 76, 207, 226, 301, 361
BmcAI AGTACT 1 cut(s) 591
Bme1390I CCNGG 3 cut(s) 81, 369, 583
Bme18I GGWCC 2 cut(s) 216, 579
BmgT120I GGNCC 2 cut(s) 216, 579
BmiI GGNNCC 1 cut(s) 218
BmrFI CCNGG 3 cut(s) 81, 369, 583
BoxI GACNNNNGTC 1 cut(s) 611
BpiI GAAGAC 2 cut(s) 266, 607
Bpu10I CCTNAGC 1 cut(s) 436
BsaJI CCNNGG 2 cut(s) 22, 367
BsaXI ACNNNNNCTCC 2 cut(s) 327, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 355
Bse1I ACTGG 1 cut(s) 102
Bse3DI GCAATG 3 cut(s) 126, 171, 234
BseBI CCWGG 3 cut(s) 81, 369, 583
BseDI CCNNGG 2 cut(s) 22, 367
BseGI GGATG 1 cut(s) 130
BseLI CCNNNNNNNGG 2 cut(s) 86, 355
BseMI GCAATG 3 cut(s) 126, 171, 234
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 348
BseXI GCAGC 4 cut(s) 211, 217, 311, 371
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 3 cut(s) 180, 372, 601
BslFI GGGAC 3 cut(s) 163, 202, 720
BslI CCNNNNNNNGG 2 cut(s) 86, 355
BsmAI GTCTC 1 cut(s) 599
BsmFI GGGAC 3 cut(s) 163, 202, 720
BsnI GGCC 3 cut(s) 180, 372, 601
Bsp143I GATC 4 cut(s) 112, 265, 316, 764
BspACI CCGC 3 cut(s) 75, 117, 653
BspANI GGCC 3 cut(s) 180, 372, 601
BspCNI CTCAG 1 cut(s) 449
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 1 cut(s) 218
BspMI ACCTGC 1 cut(s) 516
BspPI GGATC 3 cut(s) 107, 260, 759
BsrDI GCAATG 3 cut(s) 126, 171, 234
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 2 cut(s) 22, 367
BssMI GATC 4 cut(s) 112, 265, 316, 764
BssT1I CCWWGG 1 cut(s) 22
Bst2UI CCWGG 3 cut(s) 81, 369, 583
Bst4CI ACNGT 1 cut(s) 628
BstC8I GCNNGC 2 cut(s) 178, 723
BstDEI CTNAG 2 cut(s) 436, 533
BstENI CCTNNNNNAGG 1 cut(s) 84
BstF5I GGATG 1 cut(s) 130
BstFNI CGCG 1 cut(s) 77
BstHHI GCGC 2 cut(s) 79, 304
BstKTI GATC 4 cut(s) 115, 268, 319, 767
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 4 cut(s) 112, 265, 316, 764
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNI CCWGG 3 cut(s) 81, 369, 583
BstNSI RCATGY 1 cut(s) 725
BstPAI GACNNNNGTC 1 cut(s) 611
BstSCI CCNGG 3 cut(s) 79, 367, 581
BstSFI CTRYAG 1 cut(s) 624
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 4 cut(s) 211, 217, 311, 371
BstV2I GAAGAC 2 cut(s) 266, 607
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BsuRI GGCC 3 cut(s) 180, 372, 601
BtsCI GGATG 1 cut(s) 130
BveI ACCTGC 1 cut(s) 516
Cac8I GCNNGC 2 cut(s) 178, 723
CfoI GCGC 2 cut(s) 79, 304
Cfr13I GGNCC 2 cut(s) 216, 579
Csp6I GTAC 1 cut(s) 590
CviAII CATG 4 cut(s) 255, 471, 705, 722
CviQI GTAC 1 cut(s) 590
DdeI CTNAG 2 cut(s) 436, 533
DpnI GATC 4 cut(s) 114, 267, 318, 766
DpnII GATC 4 cut(s) 112, 265, 316, 764
Eco130I CCWWGG 1 cut(s) 22
Eco147I AGGCCT 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 216, 579
Eco57I CTGAAG 1 cut(s) 312
EcoNI CCTNNNNNAGG 1 cut(s) 84
EcoRII CCWGG 3 cut(s) 79, 367, 581
EcoT14I CCWWGG 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 4 cut(s) 258, 474, 708, 725
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FaqI GGGAC 3 cut(s) 163, 202, 720
FatI CATG 4 cut(s) 254, 470, 704, 721
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 5 cut(s) 75, 206, 225, 300, 360
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 5 cut(s) 75, 206, 225, 300, 360
FspBI CTAG 1 cut(s) 242
GlaI GCGC 2 cut(s) 78, 303
GluI GCNGC 5 cut(s) 75, 206, 225, 300, 360
HaeIII GGCC 3 cut(s) 180, 372, 601
HhaI GCGC 2 cut(s) 79, 304
Hin1II CATG 4 cut(s) 258, 474, 708, 725
Hin6I GCGC 2 cut(s) 77, 302
HinP1I GCGC 2 cut(s) 77, 302
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 346, 505
Hpy188I TCNGA 1 cut(s) 695
Hpy188III TCNNGA 1 cut(s) 497
Hpy8I GTNNAC 2 cut(s) 346, 505
HpyAV CCTTC 3 cut(s) 287, 498, 522
HpyCH4III ACNGT 1 cut(s) 628
HpyCH4IV ACGT 1 cut(s) 574
HpyCH4V TGCA 5 cut(s) 176, 205, 227, 314, 721
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 2 cut(s) 436, 533
HpySE526I ACGT 1 cut(s) 574
Hsp92II CATG 4 cut(s) 258, 474, 708, 725
HspAI GCGC 2 cut(s) 77, 302
Kzo9I GATC 4 cut(s) 112, 265, 316, 764
Lsp1109I GCAGC 4 cut(s) 211, 217, 311, 371
MaeI CTAG 1 cut(s) 242
MaeII ACGT 1 cut(s) 574
MaeIII GTNAC 4 cut(s) 57, 244, 566, 727
MalI GATC 4 cut(s) 114, 267, 318, 766
MboI GATC 4 cut(s) 112, 265, 316, 764
MboII GAAGA 3 cut(s) 22, 271, 607
MfeI CAATTG 1 cut(s) 781
MflI RGATCY 1 cut(s) 316
MluCI AATT 4 cut(s) 492, 516, 636, 781
MnlI CCTC 4 cut(s) 122, 326, 329, 445
MseI TTAA 3 cut(s) 456, 549, 630
MspR9I CCNGG 3 cut(s) 81, 369, 583
MunI CAATTG 1 cut(s) 781
MvaI CCWGG 3 cut(s) 81, 369, 583
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeII GATC 4 cut(s) 112, 265, 316, 764
NlaIII CATG 4 cut(s) 258, 474, 708, 725
NlaIV GGNNCC 1 cut(s) 218
NmuCI GTSAC 3 cut(s) 57, 244, 727
NspI RCATGY 1 cut(s) 725
PaeI GCATGC 1 cut(s) 725
PceI AGGCCT 1 cut(s) 180
PkrI GCNGC 5 cut(s) 76, 207, 226, 301, 361
PshAI GACNNNNGTC 1 cut(s) 611
Psp1406I AACGTT 1 cut(s) 574
Psp6I CCWGG 3 cut(s) 79, 367, 581
PspGI CCWGG 3 cut(s) 79, 367, 581
PspN4I GGNNCC 1 cut(s) 218
PspPI GGNCC 2 cut(s) 216, 579
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 1 cut(s) 591
RsaNI GTAC 1 cut(s) 590
SaqAI TTAA 3 cut(s) 456, 549, 630
SatI GCNGC 5 cut(s) 75, 206, 225, 300, 360
Sau3AI GATC 4 cut(s) 112, 265, 316, 764
Sau96I GGNCC 2 cut(s) 216, 579
ScaI AGTACT 1 cut(s) 591
ScrFI CCNGG 3 cut(s) 81, 369, 583
SetI ASST 8 cut(s) 49, 91, 414, 437, 442, 510, 577, 668
SfcI CTRYAG 1 cut(s) 624
SinI GGWCC 2 cut(s) 216, 579
SpeI ACTAGT 1 cut(s) 241
SphI GCATGC 1 cut(s) 725
Sse9I AATT 4 cut(s) 492, 516, 636, 781
SseBI AGGCCT 1 cut(s) 180
SsiI CCGC 3 cut(s) 75, 117, 653
SspMI CTAG 1 cut(s) 242
StuI AGGCCT 1 cut(s) 180
StyD4I CCNGG 3 cut(s) 79, 367, 581
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 1 cut(s) 628
TaiI ACGT 1 cut(s) 577
TaqI TCGA 1 cut(s) 560
TasI AATT 4 cut(s) 492, 516, 636, 781
TatI WGTACW 1 cut(s) 589
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 3 cut(s) 456, 549, 630
Tru9I TTAA 3 cut(s) 456, 549, 630
TseFI GTSAC 3 cut(s) 57, 244, 727
TseI GCWGC 4 cut(s) 205, 224, 299, 359
Tsp45I GTSAC 3 cut(s) 57, 244, 727
TspDTI ATGAA 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 216, 579
XagI CCTNNNNNAGG 1 cut(s) 84
XceI RCATGY 1 cut(s) 725
XspI CTAG 1 cut(s) 242
ZrmI AGTACT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.