RchiOBHm_Chr5g0002201

Belongs to the peptidase S8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016592
Physical Location & Seq
Forward (+)
1272116 .. 1272475
360 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28358

Sequence Viewer

Length: 360 bp
ATGCAAGCAAAATTAATAAACTTGCTCTTCTGTTGTTTAGGACACAGTTTTGCACTACTTTCTGGTACAACCATGGCAACACCTCAAATCGCTGGAATAGCAGCACTTATTAAGCAGTACAATCCTTCATGGACTCCTTCCATGATTGCATCTGCAATTTCCACCACGGCAACCAAGTATGATAAAAATGGAGAACTGATAATGGCTGAAGGATCTGATATAGGCAGTCAATATCCCTCCACTCCTTTTGATTTTGGAGCTGGCTTTGTCAGTCCAAGTCATGCCATGGATCCGGGTCTAGTCTTATCTTCAGGTGAAGTATACACTAGCTTTCTAGCTTGTTTGATTTTCGCTATATGA

Protein Analysis

119

Amino Acids

12.5

Weight (kDa)

4.96

Isoelectric Point (pI)

29.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 15 - 64 1.7e-11 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016592)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32980
malus_domestica MD14G1139200.v1.1
pyrus_communis pycom14g11490
rosa_chinensis RchiOBHm_Chr5g0002201
rosa_multiflora Rmu_sc0001492.1_g000003
rosa_roxburghii Rroxscaffold_3G00267180
rosa_rugosa Rorug06G0483500
rosa_samantha Rh5AG017200 Rh7AG088600 Rh7BG090000 Rh7BG090400 Rh7CG089200 Rh7CG089400 Rh7DG090800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 321
AclWI GGATC 3 cut(s) 220, 284, 297
AcuI CTGAAG 2 cut(s) 228, 294
AfaI GTAC 2 cut(s) 67, 119
AluBI AGCT 3 cut(s) 260, 330, 338
AluI AGCT 3 cut(s) 260, 330, 338
AlwI GGATC 3 cut(s) 220, 284, 297
ApeKI GCWGC 1 cut(s) 101
AseI ATTAAT 1 cut(s) 14
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 1 cut(s) 326
BamHI GGATCC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 113
BceAI ACGGC 1 cut(s) 183
BcnI CCSGG 1 cut(s) 294
BfaI CTAG 3 cut(s) 299, 327, 335
BisI GCNGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 103
Bme1390I CCNGG 1 cut(s) 294
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 1 cut(s) 294
BmsI GCATC 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 294
BsaJI CCNNGG 3 cut(s) 72, 165, 285
BseDI CCNNGG 3 cut(s) 72, 165, 285
BseXI GCAGC 1 cut(s) 113
BsiSI CCGG 1 cut(s) 293
Bsp143I GATC 2 cut(s) 212, 289
Bsp19I CCATGG 2 cut(s) 72, 285
BspLI GGNNCC 1 cut(s) 291
BspPI GGATC 3 cut(s) 220, 284, 297
BspQI GCTCTTC 1 cut(s) 32
BssECI CCNNGG 3 cut(s) 72, 165, 285
BssMI GATC 2 cut(s) 212, 289
BssNAI GTATAC 1 cut(s) 322
BssT1I CCWWGG 2 cut(s) 72, 285
Bst1107I GTATAC 1 cut(s) 322
Bst4CI ACNGT 1 cut(s) 47
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 6, 262
BstDSI CCRYGG 3 cut(s) 72, 165, 285
BstKTI GATC 2 cut(s) 215, 292
BstMBI GATC 2 cut(s) 212, 289
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 292
BstV1I GCAGC 1 cut(s) 113
BstX2I RGATCY 2 cut(s) 212, 289
BstYI RGATCY 2 cut(s) 212, 289
BstZ17I GTATAC 1 cut(s) 322
BtgI CCRYGG 3 cut(s) 72, 165, 285
Cac8I GCNNGC 2 cut(s) 6, 262
Csp6I GTAC 2 cut(s) 66, 118
CspCI CAANNNNNGTGG 2 cut(s) 229, 264
CviAII CATG 5 cut(s) 73, 129, 142, 281, 286
CviJI RGCY 5 cut(s) 206, 260, 264, 330, 338
CviKI_1 RGCY 5 cut(s) 206, 260, 264, 330, 338
CviQI GTAC 2 cut(s) 66, 118
DpnI GATC 2 cut(s) 214, 291
DpnII GATC 2 cut(s) 212, 289
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco130I CCWWGG 2 cut(s) 72, 285
Eco57I CTGAAG 2 cut(s) 228, 294
EcoT14I CCWWGG 2 cut(s) 72, 285
ErhI CCWWGG 2 cut(s) 72, 285
FaeI CATG 5 cut(s) 76, 132, 145, 284, 289
FatI CATG 5 cut(s) 72, 128, 141, 280, 285
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 102
Fsp4HI GCNGC 1 cut(s) 102
FspBI CTAG 3 cut(s) 299, 327, 335
GluI GCNGC 1 cut(s) 102
HapII CCGG 1 cut(s) 293
Hin1II CATG 5 cut(s) 76, 132, 145, 284, 289
HinfI GANTC 1 cut(s) 133
HpaII CCGG 1 cut(s) 293
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 1 cut(s) 217
Hpy8I GTNNAC 1 cut(s) 322
HpyAV CCTTC 3 cut(s) 135, 147, 203
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4V TGCA 4 cut(s) 4, 53, 149, 155
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
Hsp92II CATG 5 cut(s) 76, 132, 145, 284, 289
Kzo9I GATC 2 cut(s) 212, 289
LguI GCTCTTC 1 cut(s) 32
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 5 cut(s) 48, 78, 246, 297, 306
Lsp1109I GCAGC 1 cut(s) 113
LweI GCATC 1 cut(s) 158
MaeI CTAG 3 cut(s) 299, 327, 335
MalI GATC 2 cut(s) 214, 291
MboI GATC 2 cut(s) 212, 289
MboII GAAGA 2 cut(s) 19, 300
MflI RGATCY 2 cut(s) 212, 289
MluCI AATT 2 cut(s) 11, 156
MlyI GAGTC 1 cut(s) 127
MnlI CCTC 2 cut(s) 93, 247
MseI TTAA 2 cut(s) 14, 111
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 294
MwoI GCNNNNNNNGC 1 cut(s) 98
NciI CCSGG 1 cut(s) 294
NcoI CCATGG 2 cut(s) 72, 285
NdeII GATC 2 cut(s) 212, 289
NlaIII CATG 5 cut(s) 76, 132, 145, 284, 289
NlaIV GGNNCC 1 cut(s) 291
PciSI GCTCTTC 1 cut(s) 32
PkrI GCNGC 1 cut(s) 103
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PshBI ATTAAT 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 291
PsuI RGATCY 2 cut(s) 212, 289
RsaI GTAC 2 cut(s) 67, 119
RsaNI GTAC 2 cut(s) 66, 118
SapI GCTCTTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 14, 111
SatI GCNGC 1 cut(s) 102
Sau3AI GATC 2 cut(s) 212, 289
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 1 cut(s) 294
SetI ASST 5 cut(s) 85, 262, 316, 332, 340
SfaNI GCATC 1 cut(s) 158
Sse9I AATT 2 cut(s) 11, 156
SspMI CTAG 3 cut(s) 299, 327, 335
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 2 cut(s) 72, 285
TaaI ACNGT 1 cut(s) 47
TasI AATT 2 cut(s) 11, 156
TatI WGTACW 1 cut(s) 117
Tru1I TTAA 2 cut(s) 14, 111
Tru9I TTAA 2 cut(s) 14, 111
TseI GCWGC 1 cut(s) 101
TspDTI ATGAA 1 cut(s) 117
VspI ATTAAT 1 cut(s) 14
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 3 cut(s) 299, 327, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.