Rh7BG090000

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0016592
Physical Location & Seq
Forward (+)
6660580 .. 6661164
585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG090000.1

Sequence Viewer

Length: 585 bp
ATGACAGCATGGAAGACTGATCCATTTGGAGAGCCAATATTTGCTGAGGGGTCACCGCAAAAGCTTGCTGATCCATTTGATTATGGAGGTGGGATTGTGAACCCTAACAAGGCTGCAGACCCTGGTCTAATTTATGACATGGGCATTAATGACTACATAATTTACCTATGTGCTGTTGGGTACAACAACTCGGTAATTTCTCAACTTGTTGGGAATTCGACAACATGTTCTAGTACAAAGCCTTCTGTTCTTGATGTGAACCTGCCATCCATTACCATTCCAAATCTAAGAGAGAACATTACGCTCACCAGAAGTGTCACAAATGTTGGTCCAGTTAACTCAACATACAGAGCTCGGATAAGCCCTCCATTGGGAATCAGTGTGGCTGTGAGGCCTGAAACCTTGGTTTTCAACTCGGACATTAAAACAATCTCTTTCACAGTTGCGGTTTCTACCACCCATGAAGTGAACACAGGGTACTACTTTGGAACCCTTGTTTGGACTGATGATGATGGGGGGCATGTGGTGACAATTCCCATATCTGTTAGGACACAAATCATTCAATATTATGCAGATGGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

194

Amino Acids

20.93

Weight (kDa)

4.69

Isoelectric Point (pI)

26.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 85 - 182 4.7e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016592)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32980
malus_domestica MD14G1139200.v1.1
pyrus_communis pycom14g11490
rosa_chinensis RchiOBHm_Chr5g0002201
rosa_multiflora Rmu_sc0001492.1_g000003
rosa_roxburghii Rroxscaffold_3G00267180
rosa_rugosa Rorug06G0483500
rosa_samantha Rh5AG017200 Rh7AG088600 Rh7BG090000 Rh7BG090400 Rh7CG089200 Rh7CG089400 Rh7DG090800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 270
AciI CCGC 2 cut(s) 56, 446
AclWI GGATC 2 cut(s) 14, 65
AcsI RAATTY 1 cut(s) 214
AfaI GTAC 3 cut(s) 182, 235, 479
AfiI CCNNNNNNNGG 4 cut(s) 109, 370, 371, 498
AflIII ACRYGT 1 cut(s) 224
AgsI TTSAA 2 cut(s) 412, 563
AjnI CCWGG 1 cut(s) 121
AjuI GAANNNNNNNTTGG 2 cut(s) 481, 513
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 2 cut(s) 64, 353
AluI AGCT 2 cut(s) 64, 353
Alw21I GWGCWC 1 cut(s) 355
AlwI GGATC 2 cut(s) 14, 65
AlwNI CAGNNNCTG 1 cut(s) 122
AoxI GGCC 1 cut(s) 392
ApeKI GCWGC 1 cut(s) 113
ApoI RAATTY 1 cut(s) 214
AseI ATTAAT 1 cut(s) 147
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 3 cut(s) 45, 298, 538
AvaII GGWCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 355
BarI GAAGNNNNNNTAC 2 cut(s) 226, 258
BbsI GAAGAC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 355
BbvCI CCTCAGC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 100
BccI CCATC 3 cut(s) 274, 506, 569
BciT130I CCWGG 1 cut(s) 123
BfaI CTAG 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 114
BfuAI ACCTGC 1 cut(s) 270
BisI GCNGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 115
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 490
BmrFI CCNGG 1 cut(s) 123
BoxI GACNNNNGTC 1 cut(s) 123
BpiI GAAGAC 1 cut(s) 20
Bpu10I CCTNAGC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 121, 402
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 370, 371, 498
Bse1I ACTGG 1 cut(s) 332
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 2 cut(s) 121, 402
BseGI GGATG 1 cut(s) 266
BseLI CCNNNNNNNGG 4 cut(s) 109, 370, 371, 498
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 1 cut(s) 332
BseXI GCAGC 1 cut(s) 100
BshFI GGCC 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 355
BslI CCNNNNNNNGG 4 cut(s) 109, 370, 371, 498
BsnI GGCC 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 2 cut(s) 19, 70
BspACI CCGC 2 cut(s) 56, 446
BspANI GGCC 1 cut(s) 394
BspCNI CTCAG 1 cut(s) 37
BspLI GGNNCC 1 cut(s) 490
BspMAI CTGCAG 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 270
BspPI GGATC 2 cut(s) 14, 65
BsrI ACTGG 1 cut(s) 332
BssECI CCNNGG 2 cut(s) 121, 402
BssMI GATC 2 cut(s) 19, 70
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 442
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 2 cut(s) 45, 287
BstEII GGTNACC 1 cut(s) 51
BstF5I GGATG 1 cut(s) 266
BstKTI GATC 2 cut(s) 22, 73
BstMBI GATC 2 cut(s) 19, 70
BstNI CCWGG 1 cut(s) 123
BstNSI RCATGY 2 cut(s) 228, 524
BstPAI GACNNNNGTC 1 cut(s) 123
BstPI GGTNACC 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 114
BstV1I GCAGC 1 cut(s) 100
BstV2I GAAGAC 1 cut(s) 20
BsuRI GGCC 1 cut(s) 394
BtsCI GGATG 1 cut(s) 266
BtsIMutI CAGTG 1 cut(s) 385
BveI ACCTGC 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 66
CaiI CAGNNNCTG 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 3 cut(s) 181, 234, 478
CviAII CATG 5 cut(s) 9, 139, 225, 461, 521
CviJI RGCY 8 cut(s) 34, 64, 113, 241, 353, 363, 386, 394
CviKI_1 RGCY 8 cut(s) 34, 64, 113, 241, 353, 363, 386, 394
CviQI GTAC 3 cut(s) 181, 234, 478
DdeI CTNAG 2 cut(s) 45, 287
DpnI GATC 2 cut(s) 21, 72
DpnII GATC 2 cut(s) 19, 70
Ecl136II GAGCTC 1 cut(s) 353
Eco130I CCWWGG 1 cut(s) 402
Eco147I AGGCCT 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 329
Eco53kI GAGCTC 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 353
EcoO65I GGTNACC 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 5 cut(s) 12, 142, 228, 464, 524
FatI CATG 5 cut(s) 8, 138, 224, 460, 520
Fnu4HI GCNGC 1 cut(s) 114
FokI GGATG 1 cut(s) 253
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 1 cut(s) 114
FspBI CTAG 1 cut(s) 231
GluI GCNGC 1 cut(s) 114
HaeIII GGCC 1 cut(s) 394
Hin1II CATG 5 cut(s) 12, 142, 228, 464, 524
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 1 cut(s) 375
HpaI GTTAAC 1 cut(s) 337
HphI GGTGA 3 cut(s) 45, 298, 538
Hpy166II GTNNAC 4 cut(s) 100, 259, 337, 469
Hpy188I TCNGA 2 cut(s) 357, 418
Hpy188III TCNNGA 1 cut(s) 251
Hpy8I GTNNAC 4 cut(s) 100, 259, 337, 469
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4V TGCA 2 cut(s) 116, 572
HpyF3I CTNAG 2 cut(s) 45, 287
Hsp92II CATG 5 cut(s) 12, 142, 228, 464, 524
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 2 cut(s) 19, 70
LpnPI CCDG 7 cut(s) 108, 135, 275, 322, 345, 408, 459
Lsp1109I GCAGC 1 cut(s) 100
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 51, 316, 526
MalI GATC 2 cut(s) 21, 72
MboI GATC 2 cut(s) 19, 70
MboII GAAGA 1 cut(s) 25
MfeI CAATTG 1 cut(s) 580
MhlI GDGCHC 1 cut(s) 355
MluCI AATT 6 cut(s) 129, 159, 195, 214, 531, 580
MnlI CCTC 4 cut(s) 40, 80, 375, 384
MseI TTAA 3 cut(s) 147, 336, 423
MspR9I CCNGG 1 cut(s) 123
MunI CAATTG 1 cut(s) 580
MvaI CCWGG 1 cut(s) 123
NdeII GATC 2 cut(s) 19, 70
NlaIII CATG 5 cut(s) 12, 142, 228, 464, 524
NlaIV GGNNCC 1 cut(s) 490
NmuCI GTSAC 3 cut(s) 51, 316, 526
NspI RCATGY 2 cut(s) 228, 524
PceI AGGCCT 1 cut(s) 394
PciI ACATGT 1 cut(s) 224
PfeI GAWTC 1 cut(s) 375
PkrI GCNGC 1 cut(s) 115
PscI ACATGT 1 cut(s) 224
PshAI GACNNNNGTC 1 cut(s) 123
PshBI ATTAAT 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 121
PspEI GGTNACC 1 cut(s) 51
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 490
PspPI GGNCC 1 cut(s) 329
PsrI GAACNNNNNNTAC 2 cut(s) 461, 493
PstI CTGCAG 1 cut(s) 118
PstNI CAGNNNCTG 1 cut(s) 122
RsaI GTAC 3 cut(s) 182, 235, 479
RsaNI GTAC 3 cut(s) 181, 234, 478
SacI GAGCTC 1 cut(s) 355
SaqAI TTAA 3 cut(s) 147, 336, 423
SatI GCNGC 1 cut(s) 114
Sau3AI GATC 2 cut(s) 19, 70
Sau96I GGNCC 1 cut(s) 329
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 355
SetI ASST 6 cut(s) 66, 91, 168, 264, 355, 404
SfcI CTRYAG 1 cut(s) 114
SinI GGWCC 1 cut(s) 329
Sse9I AATT 6 cut(s) 129, 159, 195, 214, 531, 580
SseBI AGGCCT 1 cut(s) 394
SsiI CCGC 2 cut(s) 56, 446
SspI AATATT 2 cut(s) 39, 566
SspMI CTAG 1 cut(s) 231
SstI GAGCTC 1 cut(s) 355
StuI AGGCCT 1 cut(s) 394
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 1 cut(s) 442
TaqI TCGA 1 cut(s) 218
TasI AATT 6 cut(s) 129, 159, 195, 214, 531, 580
TatI WGTACW 1 cut(s) 233
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 3 cut(s) 147, 336, 423
Tru9I TTAA 3 cut(s) 147, 336, 423
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 3 cut(s) 51, 316, 526
TseI GCWGC 1 cut(s) 113
Tsp45I GTSAC 3 cut(s) 51, 316, 526
TspDTI ATGAA 1 cut(s) 477
TspRI CASTG 1 cut(s) 385
VpaK11BI GGWCC 1 cut(s) 329
VspI ATTAAT 1 cut(s) 147
XapI RAATTY 1 cut(s) 214
XceI RCATGY 2 cut(s) 228, 524
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.