Rh7CG089400

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0016592
Physical Location & Seq
Forward (+)
6747586 .. 6748266
681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG089400.1

Sequence Viewer

Length: 576 bp
ATGGATGGAGGATTCGCCTTGAGTTCAGGAACATCAATGGCAACTCCCCATATTACAGGCATTGTAGCACTTTTAAAAGCATTGCACCCAGATTGGTCTCCTGCCGCAATCAAATCAGCACTAGTAACAACAGCATGGAAGACTGAACCATTCGGAGAACCTATCTCTTCTGAAGGGTCACTGCAGAACCCAAACAAGGCAGCAAACCCTGGACTCATATATGACATGGGAACACATGACTACATAAAATACCTTTGTGCTGTTGGCTACAACACCACAGCTATCTCACAACTTGTTGGAAAAATCACAGCATGCTCCATTACTTCCATTCTCGATGTGGATTTACCATCCATTACAATTCCAAAACTAAGAAAGCAAATAACACTCACTAGAAGTGTATCGAGTGTTGGTCCTGTCAACTCAATATATAAAGCACAAATTGAGCCTCCTCCAGGCCTATATGTAGCTGTAAGACCAGTTACATTGGTCTTCAACTCCACAGTTGAGACGATGTCTTTCACGGTTACAGTTTCCACCACCCATGAAGTGAACACTATTACTACTTCGGAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

191

Amino Acids

20.21

Weight (kDa)

6.4

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 3 - 51 1.3e-12 Subtilase family
fn3_6 PF17766 113 - 184 1.5e-15 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016592)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32980
malus_domestica MD14G1139200.v1.1
pyrus_communis pycom14g11490
rosa_chinensis RchiOBHm_Chr5g0002201
rosa_multiflora Rmu_sc0001492.1_g000003
rosa_roxburghii Rroxscaffold_3G00267180
rosa_rugosa Rorug06G0483500
rosa_samantha Rh5AG017200 Rh7AG088600 Rh7BG090000 Rh7BG090400 Rh7CG089200 Rh7CG089400 Rh7DG090800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 105
AcuI CTGAAG 1 cut(s) 192
AfiI CCNNNNNNNGG 2 cut(s) 196, 452
AgsI TTSAA 1 cut(s) 493
AhlI ACTAGT 1 cut(s) 121
AjnI CCWGG 2 cut(s) 208, 451
AluBI AGCT 2 cut(s) 281, 467
AluI AGCT 2 cut(s) 281, 467
Alw26I GTCTC 2 cut(s) 102, 500
AoxI GGCC 1 cut(s) 454
ApeKI GCWGC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 410
AvaII GGWCC 1 cut(s) 410
BbsI GAAGAC 2 cut(s) 146, 481
BbvI GCAGC 1 cut(s) 212
BccI CCATC 1 cut(s) 355
BciT130I CCWGG 2 cut(s) 210, 453
BcoDI GTCTC 2 cut(s) 102, 500
BcuI ACTAGT 1 cut(s) 121
BfaI CTAG 2 cut(s) 122, 390
BfmI CTRYAG 1 cut(s) 182
BisI GCNGC 2 cut(s) 105, 201
BlsI GCNGC 2 cut(s) 106, 202
Bme1390I CCNGG 2 cut(s) 210, 453
Bme18I GGWCC 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 410
BmrFI CCNGG 2 cut(s) 210, 453
BpiI GAAGAC 2 cut(s) 146, 481
BpmI CTGGAG 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 40
BsaI GGTCTC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 208
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 452
Bse1I ACTGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 80
BseBI CCWGG 2 cut(s) 210, 453
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 2 cut(s) 10, 347
BseLI CCNNNNNNNGG 2 cut(s) 196, 452
BseMI GCAATG 1 cut(s) 80
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 438
BseXI GCAGC 1 cut(s) 212
BshFI GGCC 1 cut(s) 456
BslI CCNNNNNNNGG 2 cut(s) 196, 452
BsmAI GTCTC 2 cut(s) 102, 500
BsmBI CGTCTC 1 cut(s) 500
BsnI GGCC 1 cut(s) 456
Bso31I GGTCTC 1 cut(s) 102
BspACI CCGC 1 cut(s) 105
BspANI GGCC 1 cut(s) 456
BspMAI CTGCAG 1 cut(s) 186
BspTNI GGTCTC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 80
BsrI ACTGG 1 cut(s) 476
BssECI CCNNGG 1 cut(s) 208
Bst2UI CCWGG 2 cut(s) 210, 453
Bst4CI ACNGT 3 cut(s) 502, 523, 529
Bst6I CTCTTC 1 cut(s) 172
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 368
BstENI CCTNNNNNAGG 1 cut(s) 450
BstF5I GGATG 2 cut(s) 10, 347
BstMAI GTCTC 2 cut(s) 102, 500
BstNI CCWGG 2 cut(s) 210, 453
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 2 cut(s) 208, 451
BstSFI CTRYAG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 212
BstV2I GAAGAC 2 cut(s) 146, 481
BsuRI GGCC 1 cut(s) 456
BtsCI GGATG 2 cut(s) 10, 347
BtsI GCAGTG 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 410
CviAII CATG 5 cut(s) 135, 226, 236, 312, 542
CviJI RGCY 6 cut(s) 267, 281, 445, 456, 467, 572
CviKI_1 RGCY 6 cut(s) 267, 281, 445, 456, 467, 572
DdeI CTNAG 1 cut(s) 368
DraI TTTAAA 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
Eco147I AGGCCT 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 410
Eco57I CTGAAG 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoRII CCWGG 2 cut(s) 208, 451
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 5 cut(s) 138, 229, 239, 315, 545
FatI CATG 5 cut(s) 134, 225, 235, 311, 541
Fnu4HI GCNGC 2 cut(s) 105, 201
FokI GGATG 2 cut(s) 17, 334
Fsp4HI GCNGC 2 cut(s) 105, 201
FspBI CTAG 2 cut(s) 122, 390
GluI GCNGC 2 cut(s) 105, 201
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 1 cut(s) 456
Hin1II CATG 5 cut(s) 138, 229, 239, 315, 545
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 2 cut(s) 12, 213
Hpy166II GTNNAC 2 cut(s) 418, 550
Hpy188I TCNGA 3 cut(s) 155, 172, 568
Hpy188III TCNNGA 2 cut(s) 27, 332
Hpy8I GTNNAC 2 cut(s) 418, 550
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 3 cut(s) 502, 523, 529
HpyCH4V TGCA 2 cut(s) 85, 184
HpyF3I CTNAG 1 cut(s) 368
Hsp92II CATG 5 cut(s) 138, 229, 239, 315, 545
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 1 cut(s) 212
MaeI CTAG 2 cut(s) 122, 390
MaeIII GTNAC 4 cut(s) 124, 177, 478, 523
MboII GAAGA 3 cut(s) 151, 159, 481
MluCI AATT 2 cut(s) 357, 438
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 2 cut(s) 456, 459
MseI TTAA 1 cut(s) 74
MspR9I CCNGG 2 cut(s) 210, 453
MvaI CCWGG 2 cut(s) 210, 453
NlaIII CATG 5 cut(s) 138, 229, 239, 315, 545
NmuCI GTSAC 1 cut(s) 177
NspI RCATGY 1 cut(s) 315
PaeI GCATGC 1 cut(s) 315
PceI AGGCCT 1 cut(s) 456
PfeI GAWTC 1 cut(s) 12
PflFI GACNNNGTC 1 cut(s) 511
PkrI GCNGC 2 cut(s) 106, 202
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 208, 451
PspGI CCWGG 2 cut(s) 208, 451
PspPI GGNCC 1 cut(s) 410
PstI CTGCAG 1 cut(s) 186
PsyI GACNNNGTC 1 cut(s) 511
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 2 cut(s) 105, 201
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 2 cut(s) 210, 453
SetI ASST 4 cut(s) 163, 255, 283, 469
SfcI CTRYAG 1 cut(s) 182
SinI GGWCC 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 19
SmoI CTYRAG 1 cut(s) 19
SpeI ACTAGT 1 cut(s) 121
SphI GCATGC 1 cut(s) 315
Sse9I AATT 2 cut(s) 357, 438
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 1 cut(s) 105
SspMI CTAG 2 cut(s) 122, 390
StuI AGGCCT 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 208, 451
TaaI ACNGT 3 cut(s) 502, 523, 529
TaqI TCGA 2 cut(s) 333, 401
TasI AATT 2 cut(s) 357, 438
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 1 cut(s) 12
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 1 cut(s) 186
TseFI GTSAC 1 cut(s) 177
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 177
TspDTI ATGAA 1 cut(s) 558
TspRI CASTG 1 cut(s) 186
Tth111I GACNNNGTC 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 410
XagI CCTNNNNNAGG 1 cut(s) 450
XceI RCATGY 1 cut(s) 315
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XspI CTAG 2 cut(s) 122, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.