pycom14g11870

methyltransferase PMT18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004896
Physical Location & Seq
Forward (+)
14938679 .. 14940869
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1602 bp
ATGTTACATGTTAGGAGCTTGGCAAACCACTTCTGCCCCTGTCATATTGTTTCTGATCGATCTGAGCTCTACCAAAGGGTTGGTTGTGATGAAACGTCATCTCAATCCGGGAACAAAACCTCCTCACCCGTATCATCATCACCGCTTCGCTTGGACTTCGAAAGCCATCATCGAGTGGACGTCAACAAAACCGAGGCCGTTCAAAGGTTCTCACCGTGTGACATGTCCTACAGCGAGTACACTCCATGCCAGGATCCAAGAAGGGGGAGGAAATTTGACAGGAAAATGTTGAAATACAGAGAGAGGCACTGCCCTACCAAAGAAGAACAACTTCTTTGCCTTATTCCTGCTCCGCCAAAGTATAAGACTCCTTTCAAGTGGCCTCAGAGTCGTGATTTTGCTTGGTATGACAACATTCCCCATAGAGAACTCAGCATTGAGAAAGCTGTCCAGAACTGGATTCAAGTCGAGGGTGACAGGTTTAGATTCCCCGGGGGAGGTACCATGTTTCCGAGGGGCGCTGATGCGTACATAGATGACATTAACGCACTCATTCCTTTAACTAAGGGCAACATCAGAACTGCAATTGATACCGGCTGTGGTGTAGCCAGTTGGGGTGCTTACCTGTTGAAGAGGGACATCGTGACAATGTCTTTTGCACCGAGAGATTCGCATGCAGCGCAGGTTCAGTTTGCATTGGAGCGTGGAGTTCCCGCTATGATTGGTGTCATGGCTTCGAAGAGGCTTCCCTACCCTGCAAGGGCTTTCGATATGGCTCACTGTTCCCGCTGCTCGATCCCTTGGATGAACTATGATGGTATGTATCTCATTGAAGTGGACAGAGTTTTAAGGCCTGGTGGTTATTGGATTCTCTCCGGGCCGCCTATTAACTGGAAAAAACACTGGAGGGGATGGGAGAGAACTCAAGAGGATTTGAAGCAAGAGCAAGATTCTATTGAGTCTGTTGCAAAGCGCCTGTGCTGGAAGAAGGTGATCGAGAATTGGAATGATCTTGCAATCTGGCAAAAACCTATCAATCACGTCGAATGCGTTAAGAGCAGGAACGTGTATAAAACACCACATATATGCAAATCTGACAATCCAGATATGGCTTGGGAAAGAGACATGGAGAGTTGCATTACTCCTCTACCGGAGACAAGCGATCCTAATGACGTTGCTGGTGGGGCATTAGAGAAGTGGCCTGAACGTGCATTTGCTGTCCCTCCTAGAATCAGCAGTGGTTCAATACCAGGAATCACAGCTGAGAAACTCCGTGAGGATAATCAACTGTGGAAGGAAAGGGTGGTACATTACAAGAGGATTGTACCAATTTCCAAAGGAAGATATCGGAATGTGATGGACATGAATGCTTACCTCGGTGGATTTGCTGCAGCATTGTCAAAATATCCAGTGTGGGTTATGAACACCGTCCCTGCCAACTCAAACCAGGACACCCTTGGTGTAATTTACGAACGGGGTTTTATTGGAACATACCAGGACTGGTGTGAGGCTTTCTCAACGTACCCTAGAACATACGATCTCATCCACGCTGGTGCGATGATCGATCGATCTTCAGCATATATCAGGACAGGTGTGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

534

Amino Acids

61.09

Weight (kDa)

8.74

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 79 - 522 2.8e-198 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
Acc36I ACCTGC 1 cut(s) 673
Acc65I GGTACC 1 cut(s) 500
AccB1I GGYRCC 1 cut(s) 500
AciI CCGC 5 cut(s) 143, 353, 714, 787, 881
AclWI GGATC 4 cut(s) 248, 261, 790, 1157
AcsI RAATTY 1 cut(s) 272
AcuI CTGAAG 1 cut(s) 1557
AcyI GRCGYC 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 218
AfaI GTAC 6 cut(s) 239, 502, 530, 1308, 1326, 1523
AfiI CCNNNNNNNGG 4 cut(s) 204, 263, 497, 760
AflIII ACRYGT 3 cut(s) 7, 222, 1065
AgsI TTSAA 8 cut(s) 203, 292, 376, 464, 631, 833, 937, 1245
AjiI CACGTC 1 cut(s) 1042
AjnI CCWGG 5 cut(s) 249, 853, 1249, 1446, 1494
AleI CACNNNNGTG 1 cut(s) 1551
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 4 cut(s) 18, 67, 446, 1262
AluI AGCT 4 cut(s) 18, 67, 446, 1262
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 2 cut(s) 1116, 1148
AlwI GGATC 4 cut(s) 248, 261, 790, 1157
Ama87I CYCGRG 1 cut(s) 491
AoxI GGCC 5 cut(s) 195, 380, 851, 878, 1199
ApeKI GCWGC 4 cut(s) 677, 789, 1388, 1391
ApoI RAATTY 1 cut(s) 272
Asp700I GAANNNNTTC 1 cut(s) 330
Asp718I GGTACC 1 cut(s) 500
AspLEI GCGC 3 cut(s) 521, 682, 975
AspS9I GGNCC 1 cut(s) 878
AsuC2I CCSGG 4 cut(s) 109, 492, 493, 877
AsuHPI GGTGA 5 cut(s) 117, 132, 204, 485, 1003
AsuII TTCGAA 2 cut(s) 159, 737
AvaI CYCGRG 1 cut(s) 491
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 4 cut(s) 689, 776, 1375, 1403
BccI CCATC 4 cut(s) 174, 809, 906, 1351
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 5 cut(s) 251, 855, 1251, 1448, 1496
BcnI CCSGG 4 cut(s) 109, 492, 493, 877
BcoDI GTCTC 2 cut(s) 1116, 1148
BfaI CTAG 2 cut(s) 1227, 1527
BfmI CTRYAG 2 cut(s) 229, 1389
BfoI RGCGCY 2 cut(s) 522, 976
BfuAI ACCTGC 1 cut(s) 673
BisI GCNGC 5 cut(s) 678, 790, 881, 1389, 1392
BlsI GCNGC 5 cut(s) 679, 791, 882, 1390, 1393
Bme1390I CCNGG 9 cut(s) 109, 251, 492, 493, 855, 877, 1251, 1448, 1496
BmeT110I CYCGRG 1 cut(s) 491
BmgBI CACGTC 1 cut(s) 1042
BmgT120I GGNCC 1 cut(s) 878
BmiI GGNNCC 2 cut(s) 255, 502
BmrFI CCNGG 9 cut(s) 109, 251, 492, 493, 855, 877, 1251, 1448, 1496
BmsI GCATC 1 cut(s) 514
BplI GAGNNNNNCTC 2 cut(s) 1499, 1531
BpmI CTGGAG 1 cut(s) 925
Bpu14I TTCGAA 2 cut(s) 159, 737
BpuEI CTTGAG 1 cut(s) 909
BpuMI CCSGG 4 cut(s) 109, 492, 493, 877
Bsa29I ATCGAT 3 cut(s) 58, 1563, 1567
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 8 cut(s) 192, 490, 491, 492, 512, 800, 1375, 1456
BsaWI WCCGGW 1 cut(s) 1150
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 4 cut(s) 204, 263, 497, 760
Bse118I RCCGGY 1 cut(s) 593
Bse1I ACTGG 6 cut(s) 461, 609, 896, 908, 1409, 1505
BseBI CCWGG 5 cut(s) 251, 855, 1251, 1448, 1496
BseCI ATCGAT 3 cut(s) 58, 1563, 1567
BseDI CCNNGG 8 cut(s) 192, 490, 491, 492, 512, 800, 1375, 1456
BseGI GGATG 3 cut(s) 810, 917, 1542
BseLI CCNNNNNNNGG 4 cut(s) 204, 263, 497, 760
BseMII CTCAG 4 cut(s) 54, 398, 445, 1254
BseNI ACTGG 6 cut(s) 461, 609, 896, 908, 1409, 1505
BseRI GAGGAG 2 cut(s) 112, 1134
BseXI GCAGC 4 cut(s) 689, 776, 1375, 1403
Bsh1285I CGRYCG 1 cut(s) 1567
BshFI GGCC 5 cut(s) 197, 382, 853, 880, 1201
BshNI GGYRCC 1 cut(s) 500
BshVI ATCGAT 3 cut(s) 58, 1563, 1567
BsiEI CGRYCG 1 cut(s) 1567
BsiHKAI GWGCWC 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 491
BsiSI CCGG 5 cut(s) 108, 492, 594, 876, 1151
BslFI GGGAC 3 cut(s) 650, 1205, 1415
BslI CCNNNNNNNGG 4 cut(s) 204, 263, 497, 760
BsmAI GTCTC 2 cut(s) 1116, 1148
BsmFI GGGAC 3 cut(s) 650, 1205, 1415
BsmI GAATGC 2 cut(s) 1052, 1372
BsnI GGCC 5 cut(s) 197, 382, 853, 880, 1201
BsoBI CYCGRG 1 cut(s) 491
Bsp119I TTCGAA 2 cut(s) 159, 737
Bsp1286I GDGCHC 1 cut(s) 69
BspACI CCGC 5 cut(s) 143, 353, 714, 787, 881
BspANI GGCC 5 cut(s) 197, 382, 853, 880, 1201
BspCNI CTCAG 4 cut(s) 55, 397, 444, 1255
BspDI ATCGAT 3 cut(s) 58, 1563, 1567
BspLI GGNNCC 2 cut(s) 255, 502
BspMAI CTGCAG 1 cut(s) 1393
BspMI ACCTGC 1 cut(s) 673
BspPI GGATC 4 cut(s) 248, 261, 790, 1157
BspT104I TTCGAA 2 cut(s) 159, 737
BspT107I GGYRCC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 593
BsrI ACTGG 6 cut(s) 461, 609, 896, 908, 1409, 1505
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 8 cut(s) 192, 490, 491, 492, 512, 800, 1375, 1456
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 800, 1456
Bst2UI CCWGG 5 cut(s) 251, 855, 1251, 1448, 1496
Bst4CI ACNGT 4 cut(s) 216, 782, 1290, 1429
Bst6I CTCTTC 2 cut(s) 626, 734
BstACI GRCGYC 1 cut(s) 180
BstBI TTCGAA 2 cut(s) 159, 737
BstC8I GCNNGC 1 cut(s) 675
BstDEI CTNAG 5 cut(s) 63, 384, 431, 564, 1263
BstF5I GGATG 3 cut(s) 810, 917, 1542
BstH2I RGCGCY 2 cut(s) 522, 976
BstHHI GCGC 3 cut(s) 521, 682, 975
BstMAI GTCTC 2 cut(s) 1116, 1148
BstMCI CGRYCG 1 cut(s) 1567
BstMWI GCNNNNNNNGC 3 cut(s) 679, 1056, 1184
BstNI CCWGG 5 cut(s) 251, 855, 1251, 1448, 1496
BstNSI RCATGY 3 cut(s) 11, 226, 677
BstSCI CCNGG 9 cut(s) 107, 249, 490, 491, 853, 875, 1249, 1446, 1494
BstSFI CTRYAG 2 cut(s) 229, 1389
BstV1I GCAGC 4 cut(s) 689, 776, 1375, 1403
BstX2I RGATCY 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 80
BstYI RGATCY 1 cut(s) 253
Bsu15I ATCGAT 3 cut(s) 58, 1563, 1567
BsuRI GGCC 5 cut(s) 197, 382, 853, 880, 1201
BsuTUI ATCGAT 3 cut(s) 58, 1563, 1567
BtgZI GCGATG 1 cut(s) 1571
BtrI CACGTC 1 cut(s) 1042
BtsCI GGATG 3 cut(s) 810, 917, 1542
BtsI GCAGTG 2 cut(s) 307, 1243
BtsIMutI CAGTG 5 cut(s) 307, 778, 901, 1243, 1416
BveI ACCTGC 1 cut(s) 673
Cac8I GCNNGC 1 cut(s) 675
CfoI GCGC 3 cut(s) 521, 682, 975
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 1 cut(s) 878
Cfr9I CCCGGG 1 cut(s) 491
ClaI ATCGAT 3 cut(s) 58, 1563, 1567
Csp6I GTAC 6 cut(s) 238, 501, 529, 1307, 1325, 1522
CviAII CATG 8 cut(s) 8, 223, 246, 505, 674, 730, 1126, 1363
CviQI GTAC 6 cut(s) 238, 501, 529, 1307, 1325, 1522
DdeI CTNAG 5 cut(s) 63, 384, 431, 564, 1263
DraIII CACNNNGTG 1 cut(s) 218
Eam1104I CTCTTC 2 cut(s) 626, 734
EarI CTCTTC 2 cut(s) 626, 734
EciI GGCGGA 1 cut(s) 342
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 800, 1456
Eco147I AGGCCT 1 cut(s) 853
Eco24I GRGCYC 1 cut(s) 69
Eco32I GATATC 1 cut(s) 1346
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 1557
Eco88I CYCGRG 1 cut(s) 491
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 5 cut(s) 249, 853, 1249, 1446, 1494
EcoRV GATATC 1 cut(s) 1346
EcoT14I CCWWGG 2 cut(s) 800, 1456
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 2 cut(s) 800, 1456
FaeI CATG 8 cut(s) 11, 226, 249, 508, 677, 733, 1129, 1366
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 3 cut(s) 650, 1205, 1415
FatI CATG 8 cut(s) 7, 222, 245, 504, 673, 729, 1125, 1362
FauI CCCGC 2 cut(s) 721, 794
Fnu4HI GCNGC 5 cut(s) 678, 790, 881, 1389, 1392
FokI GGATG 3 cut(s) 817, 924, 1529
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 5 cut(s) 678, 790, 881, 1389, 1392
FspBI CTAG 2 cut(s) 1227, 1527
GlaI GCGC 3 cut(s) 520, 681, 974
GluI GCNGC 5 cut(s) 678, 790, 881, 1389, 1392
GsuI CTGGAG 1 cut(s) 925
HaeII RGCGCY 2 cut(s) 522, 976
HaeIII GGCC 5 cut(s) 197, 382, 853, 880, 1201
HapII CCGG 5 cut(s) 108, 492, 594, 876, 1151
HhaI GCGC 3 cut(s) 521, 682, 975
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 8 cut(s) 11, 226, 249, 508, 677, 733, 1129, 1366
Hin6I GCGC 3 cut(s) 519, 680, 973
HinP1I GCGC 3 cut(s) 519, 680, 973
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HpaII CCGG 5 cut(s) 108, 492, 594, 876, 1151
HphI GGTGA 5 cut(s) 117, 132, 204, 485, 1003
Hpy166II GTNNAC 4 cut(s) 178, 184, 240, 838
Hpy188I TCNGA 7 cut(s) 55, 64, 387, 513, 578, 1096, 1350
Hpy188III TCNNGA 7 cut(s) 392, 451, 643, 926, 997, 1103, 1585
Hpy8I GTNNAC 4 cut(s) 178, 184, 240, 838
Hpy99I CGWCG 1 cut(s) 1046
HpyAV CCTTC 3 cut(s) 255, 982, 1288
HpyCH4III ACNGT 4 cut(s) 216, 782, 1290, 1429
HpyCH4IV ACGT 7 cut(s) 95, 180, 1041, 1065, 1173, 1207, 1520
HpyF10VI GCNNNNNNNGC 3 cut(s) 679, 1056, 1184
HpyF3I CTNAG 5 cut(s) 63, 384, 431, 564, 1263
HpySE526I ACGT 7 cut(s) 95, 180, 1041, 1065, 1173, 1207, 1520
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 8 cut(s) 11, 226, 249, 508, 677, 733, 1129, 1366
HspAI GCGC 3 cut(s) 519, 680, 973
KpnI GGTACC 1 cut(s) 504
LmnI GCTCC 3 cut(s) 15, 355, 700
Lsp1109I GCAGC 4 cut(s) 689, 776, 1375, 1403
LweI GCATC 1 cut(s) 514
MaeI CTAG 2 cut(s) 1227, 1527
MaeII ACGT 7 cut(s) 95, 180, 1041, 1065, 1173, 1207, 1520
MaeIII GTNAC 5 cut(s) 3, 218, 473, 643, 1594
MboII GAAGA 6 cut(s) 335, 643, 751, 997, 1353, 1563
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 5 cut(s) 272, 585, 1000, 1329, 1464
MlyI GAGTC 3 cut(s) 361, 397, 968
MroXI GAANNNNTTC 1 cut(s) 330
MseI TTAA 5 cut(s) 543, 560, 848, 888, 1053
MslI CAYNNNNRTG 3 cut(s) 833, 1084, 1551
MspA1I CMGCKG 2 cut(s) 789, 1262
MspI CCGG 5 cut(s) 108, 492, 594, 876, 1151
MspR9I CCNGG 9 cut(s) 109, 251, 492, 493, 855, 877, 1251, 1448, 1496
MunI CAATTG 1 cut(s) 585
Mva1269I GAATGC 2 cut(s) 1052, 1372
MvaI CCWGG 5 cut(s) 251, 855, 1251, 1448, 1496
MwoI GCNNNNNNNGC 3 cut(s) 679, 1056, 1184
NciI CCSGG 4 cut(s) 109, 492, 493, 877
NlaIII CATG 8 cut(s) 11, 226, 249, 508, 677, 733, 1129, 1366
NlaIV GGNNCC 2 cut(s) 255, 502
NmuCI GTSAC 4 cut(s) 218, 473, 643, 1594
NspI RCATGY 3 cut(s) 11, 226, 677
NspV TTCGAA 2 cut(s) 159, 737
OliI CACNNNNGTG 1 cut(s) 1551
PaeI GCATGC 1 cut(s) 677
PceI AGGCCT 1 cut(s) 853
PciI ACATGT 2 cut(s) 7, 222
PcsI WCGNNNNNNNCGW 1 cut(s) 1047
PctI GAATGC 2 cut(s) 1052, 1372
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 7 cut(s) 460, 486, 668, 868, 950, 1230, 1254
PflFI GACNNNGTC 1 cut(s) 649
PfoI TCCNGGA 1 cut(s) 107
PkrI GCNGC 5 cut(s) 679, 791, 882, 1390, 1393
Ple19I CGATCG 1 cut(s) 1567
PleI GAGTC 3 cut(s) 361, 396, 967
PpsI GAGTC 3 cut(s) 361, 396, 967
PscI ACATGT 2 cut(s) 7, 222
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 5 cut(s) 249, 853, 1249, 1446, 1494
PspGI CCWGG 5 cut(s) 249, 853, 1249, 1446, 1494
PspN4I GGNNCC 2 cut(s) 255, 502
PspPI GGNCC 1 cut(s) 878
PstI CTGCAG 1 cut(s) 1393
PsuI RGATCY 1 cut(s) 253
PsyI GACNNNGTC 1 cut(s) 649
PvuI CGATCG 1 cut(s) 1567
PvuII CAGCTG 1 cut(s) 1262
RsaI GTAC 6 cut(s) 239, 502, 530, 1308, 1326, 1523
RsaNI GTAC 6 cut(s) 238, 501, 529, 1307, 1325, 1522
RseI CAYNNNNRTG 3 cut(s) 833, 1084, 1551
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 5 cut(s) 543, 560, 848, 888, 1053
SatI GCNGC 5 cut(s) 678, 790, 881, 1389, 1392
Sau96I GGNCC 1 cut(s) 878
SchI GAGTC 3 cut(s) 361, 397, 968
ScrFI CCNGG 9 cut(s) 109, 251, 492, 493, 855, 877, 1251, 1448, 1496
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 2 cut(s) 229, 1389
SfuI TTCGAA 2 cut(s) 159, 737
SmaI CCCGGG 1 cut(s) 493
SmiMI CAYNNNNRTG 3 cut(s) 833, 1084, 1551
SmlI CTYRAG 1 cut(s) 924
SmoI CTYRAG 1 cut(s) 924
SphI GCATGC 1 cut(s) 677
Sse9I AATT 5 cut(s) 272, 585, 1000, 1329, 1464
SseBI AGGCCT 1 cut(s) 853
SsiI CCGC 5 cut(s) 143, 353, 714, 787, 881
SspMI CTAG 2 cut(s) 1227, 1527
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 853
StyD4I CCNGG 9 cut(s) 107, 249, 490, 491, 853, 875, 1249, 1446, 1494
StyI CCWWGG 2 cut(s) 800, 1456
TaaI ACNGT 4 cut(s) 216, 782, 1290, 1429
TaiI ACGT 7 cut(s) 98, 183, 1044, 1068, 1176, 1210, 1523
TasI AATT 5 cut(s) 272, 585, 1000, 1329, 1464
TatI WGTACW 1 cut(s) 237
TauI GCSGC 1 cut(s) 883
TfiI GAWTC 7 cut(s) 460, 486, 668, 868, 950, 1230, 1254
Tru1I TTAA 5 cut(s) 543, 560, 848, 888, 1053
Tru9I TTAA 5 cut(s) 543, 560, 848, 888, 1053
TscAI CASTG 5 cut(s) 314, 785, 908, 1243, 1416
TseFI GTSAC 4 cut(s) 218, 473, 643, 1594
TseI GCWGC 4 cut(s) 677, 789, 1388, 1391
Tsp45I GTSAC 4 cut(s) 218, 473, 643, 1594
TspDTI ATGAA 4 cut(s) 105, 821, 1379, 1436
TspGWI ACGGA 1 cut(s) 1262
TspMI CCCGGG 1 cut(s) 491
TspRI CASTG 5 cut(s) 314, 785, 908, 1243, 1416
Tth111I GACNNNGTC 1 cut(s) 649
XapI RAATTY 1 cut(s) 272
XceI RCATGY 3 cut(s) 11, 226, 677
XcmI CCANNNNNNNNNTGG 2 cut(s) 1110, 1454
XmaI CCCGGG 1 cut(s) 491
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 2 cut(s) 1227, 1527
ZraI GACGTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.