pycom14g11880

methyltransferase PMT18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004896
Physical Location & Seq
Forward (+)
14943911 .. 14945784
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1245 bp
ATGTTTCCGAGAGGAGCTGATGAGTACATAGATGGCATTAACGCGCTCATTCCTCTCACTAAGGGCAACATCAGAACCGCAATTGATACCGGCTGTGGTGTAGCCGGTTGGGGTGCGTACCTGTTGAAGAGGGACATCATGACAATGTCTTTTGCACCGAGAGATTCGCATGCAGCGCAGGTTCAGTTTGCATTGGAGCGCGGATTTCCTGCTATGATTGGTGTTATGGCTTCGAAGAGGCTTCCCTACCCTGCAAGGGCTTTCGATATAGCTCACTGTTCCCGCTGCTTGATCCCTTGGATGAACTATGATGGTATGTATCTAATTGAAGTGGACAGAGTTCTAAGGCCTGGTGGTTATTGGATTCTTTCCGGACCACCTATTAACTGGAAAAAATACTGGAGGGGATGGGAGAGAACTCAAGAGGATTTGAAACAAGAGCAAGATTCTATTGAGTCTGTTGCAAAGCGCCTGTGCTGGAAGAAGGTGATCGAGAAGAATGATCTTGCAATCTGGCAAAAACCTATCAATCACGTCGAATGCATTAAGAGCAGGAAGGTGTATAAAACACCATATATATACAAATCAGACAATCCAGATATGGGAAAGAGACATGGAGAGTTGCATTACTCCTCTACCGGAGACAGAGTGATCCGTATGACGTTGCTGGCGTTGGAGAACTGGCCTGAACGTGCATTTGCTGTCCCTCCTAGAATCAGCAGTGGTTCAATACCAGGAATCACGGCTGAGAAACTCCGCAAGGATAATCAACCGTGGAAGGAAAGGGTGTATCATTACAAGAGGATCGAACCAATTTCCAAAGGAAGATATCGGAATGTGATGGACATGAATGCTTACCTCGGTGGATTTGCTGCAGCATTGTCAAAATATCCAGTGTGGGTTATGAACACCGTCCTTGCCAACTTAAACCAGGACACTCTTGGTATGATTTACGAACGTGGTTTTATTGGAACATACCAGGACTGGTGTGAGGCTTTCTCAACGTATCCTAGAACATACGATCTCATCCACGCTGGTGGAGTCTTCAGCATATATCAGGACAGGTGTGACAGAACACTCATTTTGCTGGAGATGGATAGGATTCTGAGGCTGGAAGGAACAGTTGTGTTTAGGGATACAGTAGAGATCCTACCAAATTATGGATCACGAGAGCCTTTTAACCTAGAGAAAATTCTTCTTGCAACGAAAACTTACTGGACTGGTGAAGAAAAAAAGGAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

415

Amino Acids

47.83

Weight (kDa)

9.07

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 1 - 385 1.2e-154 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 169
AccB7I CCANNNNNTGG 1 cut(s) 1160
AccII CGCG 2 cut(s) 44, 201
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 4 cut(s) 78, 201, 283, 757
AclWI GGATC 5 cut(s) 286, 646, 812, 1141, 1171
AcsI RAATTY 1 cut(s) 1191
AcuI CTGAAG 1 cut(s) 1030
AfaI GTAC 2 cut(s) 26, 119
AfiI CCNNNNNNNGG 3 cut(s) 256, 602, 1160
AgsI TTSAA 4 cut(s) 127, 329, 433, 729
AjiI CACGTC 1 cut(s) 535
AjnI CCWGG 4 cut(s) 349, 733, 930, 978
AleI CACNNNNGTG 1 cut(s) 1035
AluBI AGCT 3 cut(s) 17, 272, 1242
AluI AGCT 3 cut(s) 17, 272, 1242
Alw26I GTCTC 2 cut(s) 604, 636
AlwI GGATC 5 cut(s) 286, 646, 812, 1141, 1171
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 2 cut(s) 347, 683
ApeKI GCWGC 4 cut(s) 173, 285, 872, 875
ApoI RAATTY 1 cut(s) 1191
AspLEI GCGC 4 cut(s) 46, 178, 201, 471
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 2 cut(s) 499, 1235
AsuII TTCGAA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 374
BauI CACGAG 1 cut(s) 1167
BbsI GAAGAC 1 cut(s) 1036
BbvI GCAGC 4 cut(s) 185, 272, 859, 887
BccI CCATC 5 cut(s) 26, 305, 402, 835, 1087
BceAI ACGGC 1 cut(s) 759
BciT130I CCWGG 4 cut(s) 351, 735, 932, 980
BciVI GTATCC 2 cut(s) 1017, 1129
BcoDI GTCTC 2 cut(s) 604, 636
BfaI CTAG 4 cut(s) 711, 1011, 1184, 1243
BfmI CTRYAG 1 cut(s) 873
BfoI RGCGCY 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 169
BfuI GTATCC 2 cut(s) 1017, 1129
BisI GCNGC 4 cut(s) 174, 286, 873, 876
BlsI GCNGC 4 cut(s) 175, 287, 874, 877
Bme1390I CCNGG 4 cut(s) 351, 735, 932, 980
Bme18I GGWCC 1 cut(s) 374
BmgBI CACGTC 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 374
BmrFI CCNGG 4 cut(s) 351, 735, 932, 980
BpiI GAAGAC 1 cut(s) 1036
BplI GAGNNNNNCTC 2 cut(s) 983, 1015
BpmI CTGGAG 2 cut(s) 421, 1109
Bpu14I TTCGAA 1 cut(s) 233
BpuEI CTTGAG 1 cut(s) 405
BsaJI CCNNGG 3 cut(s) 296, 773, 859
BsaWI WCCGGW 2 cut(s) 371, 638
BsaXI ACNNNNNCTCC 2 cut(s) 633, 663
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 602, 1160
Bse118I RCCGGY 2 cut(s) 89, 104
Bse1I ACTGG 7 cut(s) 392, 404, 686, 893, 989, 1220, 1225
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 4 cut(s) 351, 735, 932, 980
BseDI CCNNGG 3 cut(s) 296, 773, 859
BseGI GGATG 3 cut(s) 306, 413, 1026
BseLI CCNNNNNNNGG 3 cut(s) 256, 602, 1160
BseMII CTCAG 2 cut(s) 738, 1097
BseNI ACTGG 7 cut(s) 392, 404, 686, 893, 989, 1220, 1225
BseRI GAGGAG 2 cut(s) 27, 622
BseXI GCAGC 4 cut(s) 185, 272, 859, 887
Bsh1236I CGCG 2 cut(s) 44, 201
BshFI GGCC 2 cut(s) 349, 685
BsiSI CCGG 4 cut(s) 90, 105, 372, 639
BslFI GGGAC 2 cut(s) 146, 689
BslI CCNNNNNNNGG 3 cut(s) 256, 602, 1160
BsmAI GTCTC 2 cut(s) 604, 636
BsmFI GGGAC 2 cut(s) 146, 689
BsmI GAATGC 2 cut(s) 545, 856
BsnI GGCC 2 cut(s) 349, 685
Bsp119I TTCGAA 1 cut(s) 233
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 8 cut(s) 291, 489, 502, 651, 804, 1021, 1146, 1163
BspACI CCGC 4 cut(s) 78, 201, 283, 757
BspANI GGCC 2 cut(s) 349, 685
BspCNI CTCAG 2 cut(s) 739, 1098
BspEI TCCGGA 1 cut(s) 371
BspFNI CGCG 2 cut(s) 44, 201
BspHI TCATGA 1 cut(s) 138
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 5 cut(s) 286, 646, 812, 1141, 1171
BspT104I TTCGAA 1 cut(s) 233
BsrFI RCCGGY 2 cut(s) 89, 104
BsrI ACTGG 7 cut(s) 392, 404, 686, 893, 989, 1220, 1225
BssAI RCCGGY 2 cut(s) 89, 104
BssECI CCNNGG 3 cut(s) 296, 773, 859
BssMI GATC 8 cut(s) 291, 489, 502, 651, 804, 1021, 1146, 1163
BssSI CACGAG 1 cut(s) 1167
BssT1I CCWWGG 1 cut(s) 296
Bst2BI CACGAG 1 cut(s) 1167
Bst2UI CCWGG 4 cut(s) 351, 735, 932, 980
Bst4CI ACNGT 5 cut(s) 278, 774, 913, 1123, 1141
Bst6I CTCTTC 2 cut(s) 122, 230
BstBI TTCGAA 1 cut(s) 233
BstC8I GCNNGC 2 cut(s) 171, 669
BstDEI CTNAG 4 cut(s) 60, 344, 747, 1106
BstDSI CCRYGG 1 cut(s) 773
BstF5I GGATG 3 cut(s) 306, 413, 1026
BstFNI CGCG 2 cut(s) 44, 201
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 4 cut(s) 46, 178, 201, 471
BstKTI GATC 8 cut(s) 294, 492, 505, 654, 807, 1024, 1149, 1166
BstMAI GTCTC 2 cut(s) 604, 636
BstMBI GATC 8 cut(s) 291, 489, 502, 651, 804, 1021, 1146, 1163
BstMWI GCNNNNNNNGC 2 cut(s) 175, 549
BstNI CCWGG 4 cut(s) 351, 735, 932, 980
BstNSI RCATGY 1 cut(s) 173
BstSCI CCNGG 4 cut(s) 349, 733, 930, 978
BstSFI CTRYAG 1 cut(s) 873
BstUI CGCG 2 cut(s) 44, 201
BstV1I GCAGC 4 cut(s) 185, 272, 859, 887
BstV2I GAAGAC 1 cut(s) 1036
BstX2I RGATCY 1 cut(s) 1146
BstXI CCANNNNNNTGG 1 cut(s) 1037
BstYI RGATCY 1 cut(s) 1146
BsuI GTATCC 2 cut(s) 1017, 1129
BsuRI GGCC 2 cut(s) 349, 685
BtgI CCRYGG 1 cut(s) 773
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 3 cut(s) 306, 413, 1026
BtsI GCAGTG 1 cut(s) 727
BtsIMutI CAGTG 3 cut(s) 274, 727, 900
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 171, 669
CciI TCATGA 1 cut(s) 138
CfoI GCGC 4 cut(s) 46, 178, 201, 471
Cfr10I RCCGGY 2 cut(s) 89, 104
Cfr13I GGNCC 1 cut(s) 374
Csp6I GTAC 2 cut(s) 25, 118
CviAII CATG 4 cut(s) 139, 170, 614, 847
CviQI GTAC 2 cut(s) 25, 118
DdeI CTNAG 4 cut(s) 60, 344, 747, 1106
DpnI GATC 8 cut(s) 293, 491, 504, 653, 806, 1023, 1148, 1165
DpnII GATC 8 cut(s) 291, 489, 502, 651, 804, 1021, 1146, 1163
Eam1104I CTCTTC 2 cut(s) 122, 230
EarI CTCTTC 2 cut(s) 122, 230
Eco130I CCWWGG 1 cut(s) 296
Eco147I AGGCCT 1 cut(s) 349
Eco32I GATATC 1 cut(s) 830
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 1030
EcoRII CCWGG 4 cut(s) 349, 733, 930, 978
EcoRV GATATC 1 cut(s) 830
EcoT14I CCWWGG 1 cut(s) 296
EcoT22I ATGCAT 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 4 cut(s) 142, 173, 617, 850
FaqI GGGAC 2 cut(s) 146, 689
FatI CATG 4 cut(s) 138, 169, 613, 846
FauI CCCGC 1 cut(s) 290
Fnu4HI GCNGC 4 cut(s) 174, 286, 873, 876
FokI GGATG 3 cut(s) 313, 420, 1013
Fsp4HI GCNGC 4 cut(s) 174, 286, 873, 876
FspBI CTAG 4 cut(s) 711, 1011, 1184, 1243
GlaI GCGC 4 cut(s) 45, 177, 200, 470
GluI GCNGC 4 cut(s) 174, 286, 873, 876
GsuI CTGGAG 2 cut(s) 421, 1109
HaeII RGCGCY 1 cut(s) 472
HaeIII GGCC 2 cut(s) 349, 685
HapII CCGG 4 cut(s) 90, 105, 372, 639
HhaI GCGC 4 cut(s) 46, 178, 201, 471
Hin1II CATG 4 cut(s) 142, 173, 617, 850
Hin6I GCGC 4 cut(s) 44, 176, 199, 469
HinP1I GCGC 4 cut(s) 44, 176, 199, 469
HinfI GANTC 8 cut(s) 164, 364, 446, 455, 714, 738, 1041, 1102
HpaII CCGG 4 cut(s) 90, 105, 372, 639
HphI GGTGA 2 cut(s) 499, 1235
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 5 cut(s) 9, 74, 589, 834, 1107
Hpy188III TCNNGA 7 cut(s) 139, 372, 422, 493, 596, 1058, 1167
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 1 cut(s) 539
HpyAV CCTTC 4 cut(s) 478, 550, 772, 1109
HpyCH4III ACNGT 5 cut(s) 278, 774, 913, 1123, 1141
HpyCH4IV ACGT 5 cut(s) 534, 662, 691, 958, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 549
HpyF3I CTNAG 4 cut(s) 60, 344, 747, 1106
HpySE526I ACGT 5 cut(s) 534, 662, 691, 958, 1004
Hsp92II CATG 4 cut(s) 142, 173, 617, 850
HspAI GCGC 4 cut(s) 44, 176, 199, 469
Kpn2I TCCGGA 1 cut(s) 371
Kzo9I GATC 8 cut(s) 291, 489, 502, 651, 804, 1021, 1146, 1163
LmnI GCTCC 2 cut(s) 14, 196
Lsp1109I GCAGC 4 cut(s) 185, 272, 859, 887
MaeI CTAG 4 cut(s) 711, 1011, 1184, 1243
MaeII ACGT 5 cut(s) 534, 662, 691, 958, 1004
MaeIII GTNAC 1 cut(s) 1067
MalI GATC 8 cut(s) 293, 491, 504, 653, 806, 1023, 1148, 1165
MboI GATC 8 cut(s) 291, 489, 502, 651, 804, 1021, 1146, 1163
MboII GAAGA 8 cut(s) 139, 247, 493, 508, 837, 1036, 1187, 1238
MfeI CAATTG 1 cut(s) 81
MflI RGATCY 1 cut(s) 1146
MluCI AATT 5 cut(s) 81, 324, 813, 1156, 1191
MlyI GAGTC 2 cut(s) 464, 1050
MmeI TCCRAC 1 cut(s) 654
Mph1103I ATGCAT 1 cut(s) 545
MroI TCCGGA 1 cut(s) 371
MseI TTAA 5 cut(s) 39, 384, 546, 926, 1179
MslI CAYNNNNRTG 2 cut(s) 143, 1035
MspA1I CMGCKG 1 cut(s) 285
MspI CCGG 4 cut(s) 90, 105, 372, 639
MspR9I CCNGG 4 cut(s) 351, 735, 932, 980
MunI CAATTG 1 cut(s) 81
Mva1269I GAATGC 2 cut(s) 545, 856
MvaI CCWGG 4 cut(s) 351, 735, 932, 980
MvnI CGCG 2 cut(s) 44, 201
MwoI GCNNNNNNNGC 2 cut(s) 175, 549
NdeII GATC 8 cut(s) 291, 489, 502, 651, 804, 1021, 1146, 1163
NlaIII CATG 4 cut(s) 142, 173, 617, 850
NmuCI GTSAC 1 cut(s) 1067
NsiI ATGCAT 1 cut(s) 545
NspI RCATGY 1 cut(s) 173
NspV TTCGAA 1 cut(s) 233
OliI CACNNNNGTG 1 cut(s) 1035
PaeI GCATGC 1 cut(s) 173
PagI TCATGA 1 cut(s) 138
PceI AGGCCT 1 cut(s) 349
PcsI WCGNNNNNNNCGW 1 cut(s) 668
PctI GAATGC 2 cut(s) 545, 856
PfeI GAWTC 6 cut(s) 164, 364, 446, 714, 738, 1102
PflFI GACNNNGTC 1 cut(s) 145
PflMI CCANNNNNTGG 1 cut(s) 1160
PkrI GCNGC 4 cut(s) 175, 287, 874, 877
PleI GAGTC 2 cut(s) 463, 1049
PpsI GAGTC 2 cut(s) 463, 1049
Psp6I CCWGG 4 cut(s) 349, 733, 930, 978
PspGI CCWGG 4 cut(s) 349, 733, 930, 978
PspPI GGNCC 1 cut(s) 374
PstI CTGCAG 1 cut(s) 877
PsuI RGATCY 1 cut(s) 1146
PsyI GACNNNGTC 1 cut(s) 145
RsaI GTAC 2 cut(s) 26, 119
RsaNI GTAC 2 cut(s) 25, 118
RseI CAYNNNNRTG 2 cut(s) 143, 1035
SaqAI TTAA 5 cut(s) 39, 384, 546, 926, 1179
SatI GCNGC 4 cut(s) 174, 286, 873, 876
Sau3AI GATC 8 cut(s) 291, 489, 502, 651, 804, 1021, 1146, 1163
Sau96I GGNCC 1 cut(s) 374
SchI GAGTC 2 cut(s) 464, 1050
ScrFI CCNGG 4 cut(s) 351, 735, 932, 980
SfcI CTRYAG 1 cut(s) 873
SfuI TTCGAA 1 cut(s) 233
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 2 cut(s) 143, 1035
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
SphI GCATGC 1 cut(s) 173
Sse9I AATT 5 cut(s) 81, 324, 813, 1156, 1191
SseBI AGGCCT 1 cut(s) 349
SsiI CCGC 4 cut(s) 78, 201, 283, 757
SspMI CTAG 4 cut(s) 711, 1011, 1184, 1243
StuI AGGCCT 1 cut(s) 349
StyD4I CCNGG 4 cut(s) 349, 733, 930, 978
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 5 cut(s) 278, 774, 913, 1123, 1141
TaiI ACGT 5 cut(s) 537, 665, 694, 961, 1007
TaqI TCGA 5 cut(s) 233, 264, 492, 537, 807
TasI AATT 5 cut(s) 81, 324, 813, 1156, 1191
TatI WGTACW 1 cut(s) 24
TfiI GAWTC 6 cut(s) 164, 364, 446, 714, 738, 1102
Tru1I TTAA 5 cut(s) 39, 384, 546, 926, 1179
Tru9I TTAA 5 cut(s) 39, 384, 546, 926, 1179
TscAI CASTG 3 cut(s) 281, 727, 900
TseFI GTSAC 1 cut(s) 1067
TseI GCWGC 4 cut(s) 173, 285, 872, 875
Tsp45I GTSAC 1 cut(s) 1067
TspDTI ATGAA 3 cut(s) 317, 863, 920
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 3 cut(s) 281, 727, 900
Tth111I GACNNNGTC 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 1160
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 1 cut(s) 1191
XceI RCATGY 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 2 cut(s) 384, 938
XspI CTAG 4 cut(s) 711, 1011, 1184, 1243
Zsp2I ATGCAT 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.