pycom14g12420

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011298
Physical Location & Seq
Forward (+)
15427158 .. 15429338
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1233 bp
ATGTCTCCCTTCTCTCCACTTCCCGGCCCCTCCTTACCTTTATATAAGCCACTTCCCACTCGGTTTCTCGCAGACAACCCACTTTTCTTGACTTACAACTCTCTCAACCATTTTTCCAATTTTTCAAAAGCTCTCTCTCTCTCCAGATATATATATTGTTATACAATGGCAGCCTTTTCATACAATGGCAGCCTTGACTCAATTTTCTTGCCCAACGCTCCGGCTACCAATGACATCATCATTTACAGTAACTTCTTTTCCCCATCTGAGCCTTTAACCCGGGAAATTCAAGCTGATAATAATTCAAGAGCTGTTGAAAGCAGCTGCACTATGGATCGCAGCTCCACTCATAAGGTTGCTTTTAGTGACAATGAGAATGAGCCTTCTGTGACAAAAAACAAGAGCACAGAGTCCTCAACTGTTGTGGATGTGGATAGGCTTGAAATTGGTGAGCAGGTCACTCAGAAAGTAACTCCTATGGACAAGAAGAGAAAATTCAGAATTGGGTCATCCTTCAAATCTGCTCAATCCAAGGGCACTCAAGAGCATAAGGGAAAGAAGCAAAAGAAGATTGGTGGCGGTACCAAGAAAGCTGAGGAAAAGAAGATTAAATCAGATAAAAAAGACCAGGTGAAAGTAAGAGAAGAACCTCCAACTGGCTACATTCATGTTAGAGCAAGGAGGGGTGAGGCTACAGATAGTCACAGCCTTGCAGAAAGGGTAAGAAGAGAGAAAATTAGTGAGAGGATGAAGATGCTGCAAAAACTTGTCCCTGGCTGTGACAAGGTAACTGGAAGGGCAGTCATGCTGGATGAAATTATCAATTATGTTCAGTCCCTACAAAATCAAGTGGAGTTCCTCTCACTGAAACTTGCTTCTTCGAATCCAATGTTCTTTGAATTTGGAATGGACATGGGTGCTTTAGTGGTTAAACCAGAGAGATTGTGTAGCATGGAATCACCATATCCACCTGCGCAACAATGCAACCCAATACAGTCCTCACCTTTTGCTGATACATCCACCAACATCACTGCCACCACTGCCGCTGCCACTGCCGCCATCACCACAAATAACAACTATCACTTTCTGGACAATTCAGCTTCACATCTACTTCAAAGGCCAAATACCTTCTCTCAGGACAGTGAGAGCTTGTTGTGGGATGTGGAAGATCAAAGACAAAACTTTCATAATCCATGTGGTTTCAGCAACAGCAACTTGTGTTCTTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

411

Amino Acids

45.85

Weight (kDa)

8.79

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 234 - 281 4.2e-06 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 239 - 283 1.4e-06 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011298)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50915 AT5G50915 AT5G50915 AT5G50915
fragaria_vesca FvH4_5g09140 FvH4_5g09140
malus_domestica MD06G1133200.v1.1 MD14G1149600.v1.1
prunus_persica Prupe.5G144400_v2.0.a1
pyrus_communis pycom06g12480 pycom14g12420
rosa_chinensis RchiOBHm_Chr7g0189021
rosa_laevigata RLG00000004638
rosa_roxburghii Rroxscaffold_3G00265840
rosa_rugosa Rorug06G0495700
rosa_samantha Rh7AG100200 Rh7BG102700 Rh7CG104100 Rh7DG102200
rosa_wichuraiana Rw7G008660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 979
Acc16I TGCGCA 1 cut(s) 975
Acc36I ACCTGC 2 cut(s) 445, 979
Acc65I GGTACC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 581
AciI CCGC 3 cut(s) 579, 1044, 1056
AclWI GGATC 1 cut(s) 342
AcsI RAATTY 3 cut(s) 285, 494, 899
AfaI GTAC 1 cut(s) 583
AfiI CCNNNNNNNGG 2 cut(s) 23, 656
AgsI TTSAA 9 cut(s) 126, 290, 306, 317, 443, 517, 899, 1115, 1228
AjnI CCWGG 2 cut(s) 627, 772
AluBI AGCT 8 cut(s) 131, 293, 311, 324, 342, 593, 1100, 1149
AluI AGCT 8 cut(s) 131, 293, 311, 324, 342, 593, 1100, 1149
Alw21I GWGCWC 1 cut(s) 407
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 342
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 2 cut(s) 25, 1118
ApeKI GCWGC 7 cut(s) 170, 189, 321, 324, 339, 757, 1046
ApoI RAATTY 3 cut(s) 285, 494, 899
Asp718I GGTACC 1 cut(s) 581
AspLEI GCGC 1 cut(s) 976
AspS9I GGNCC 1 cut(s) 26
AsuC2I CCSGG 3 cut(s) 24, 280, 281
AsuHPI GGTGA 6 cut(s) 461, 643, 698, 951, 993, 1054
AsuII TTCGAA 1 cut(s) 881
AvaI CYCGRG 1 cut(s) 279
BaeGI GKGCMC 1 cut(s) 539
BanI GGYRCC 1 cut(s) 581
Bbv12I GWGCWC 1 cut(s) 407
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 7 cut(s) 182, 201, 311, 333, 351, 744, 1033
BccI CCATC 2 cut(s) 271, 1067
BciT130I CCWGG 2 cut(s) 629, 774
BcnI CCSGG 3 cut(s) 24, 280, 281
BcoDI GTCTC 1 cut(s) 9
BfmI CTRYAG 1 cut(s) 693
BfuAI ACCTGC 2 cut(s) 445, 979
BisI GCNGC 9 cut(s) 171, 190, 322, 325, 340, 758, 1044, 1047, 1056
BlsI GCNGC 9 cut(s) 172, 191, 323, 326, 341, 759, 1045, 1048, 1057
Bme1390I CCNGG 5 cut(s) 24, 280, 281, 629, 774
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 2 cut(s) 28, 583
BmrFI CCNGG 5 cut(s) 24, 280, 281, 629, 774
BmsI GCATC 1 cut(s) 744
BplI GAGNNNNNCTC 2 cut(s) 845, 877
BpmI CTGGAG 1 cut(s) 127
Bpu10I CCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 881
BpuEI CTTGAG 1 cut(s) 525
BpuMI CCSGG 3 cut(s) 24, 280, 281
BsaJI CCNNGG 3 cut(s) 279, 531, 772
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 656
Bse1I ACTGG 2 cut(s) 661, 796
BseBI CCWGG 2 cut(s) 629, 774
BseDI CCNNGG 3 cut(s) 279, 531, 772
BseGI GGATG 6 cut(s) 433, 509, 753, 817, 1016, 1165
BseLI CCNNNNNNNGG 2 cut(s) 23, 656
BseMII CTCAG 4 cut(s) 258, 476, 585, 1148
BseNI ACTGG 2 cut(s) 661, 796
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 7 cut(s) 182, 201, 311, 333, 351, 744, 1033
BsgI GTGCAG 1 cut(s) 310
BshFI GGCC 2 cut(s) 27, 1120
BshNI GGYRCC 1 cut(s) 581
BsiHKAI GWGCWC 1 cut(s) 407
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 3 cut(s) 24, 221, 280
BslFI GGGAC 2 cut(s) 755, 820
BslI CCNNNNNNNGG 2 cut(s) 23, 656
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 755, 820
BsnI GGCC 2 cut(s) 27, 1120
BsoBI CYCGRG 1 cut(s) 279
Bsp119I TTCGAA 1 cut(s) 881
Bsp1286I GDGCHC 2 cut(s) 407, 539
Bsp143I GATC 2 cut(s) 334, 1168
BspACI CCGC 3 cut(s) 579, 1044, 1056
BspANI GGCC 2 cut(s) 27, 1120
BspCNI CTCAG 4 cut(s) 259, 475, 586, 1147
BspLI GGNNCC 2 cut(s) 28, 583
BspMI ACCTGC 2 cut(s) 445, 979
BspPI GGATC 1 cut(s) 342
BspT104I TTCGAA 1 cut(s) 881
BspT107I GGYRCC 1 cut(s) 581
BsrI ACTGG 2 cut(s) 661, 796
BssECI CCNNGG 3 cut(s) 279, 531, 772
BssMI GATC 2 cut(s) 334, 1168
BssT1I CCWWGG 1 cut(s) 531
Bst2UI CCWGG 2 cut(s) 629, 774
Bst4CI ACNGT 4 cut(s) 248, 421, 996, 1142
Bst6I CTCTTC 2 cut(s) 482, 721
BstBI TTCGAA 1 cut(s) 881
BstDEI CTNAG 4 cut(s) 267, 462, 594, 1134
BstF5I GGATG 6 cut(s) 433, 509, 753, 817, 1016, 1165
BstHHI GCGC 1 cut(s) 976
BstKTI GATC 2 cut(s) 337, 1171
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 334, 1168
BstMWI GCNNNNNNNGC 3 cut(s) 1040, 1052, 1055
BstNI CCWGG 2 cut(s) 629, 774
BstSCI CCNGG 5 cut(s) 22, 278, 279, 627, 772
BstSFI CTRYAG 1 cut(s) 693
BstSLI GKGCMC 1 cut(s) 539
BstV1I GCAGC 7 cut(s) 182, 201, 311, 333, 351, 744, 1033
BsuRI GGCC 2 cut(s) 27, 1120
BtsCI GGATG 6 cut(s) 433, 509, 753, 817, 1016, 1165
BtsI GCAGTG 3 cut(s) 1029, 1038, 1050
BtsIMutI CAGTG 5 cut(s) 863, 1029, 1038, 1050, 1147
BveI ACCTGC 2 cut(s) 445, 979
CfoI GCGC 1 cut(s) 976
Cfr13I GGNCC 1 cut(s) 26
Cfr9I CCCGGG 1 cut(s) 279
CsiI ACCWGGT 1 cut(s) 627
Csp6I GTAC 1 cut(s) 582
CspCI CAANNNNNGTGG 4 cut(s) 69, 104, 405, 440
CviAII CATG 5 cut(s) 668, 805, 913, 952, 1194
CviQI GTAC 1 cut(s) 582
DdeI CTNAG 4 cut(s) 267, 462, 594, 1134
DpnI GATC 2 cut(s) 336, 1170
DpnII GATC 2 cut(s) 334, 1168
Eam1104I CTCTTC 2 cut(s) 482, 721
EarI CTCTTC 2 cut(s) 482, 721
Eco130I CCWWGG 1 cut(s) 531
Eco88I CYCGRG 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 627, 772
EcoT14I CCWWGG 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 5 cut(s) 671, 808, 916, 955, 1197
FaqI GGGAC 2 cut(s) 755, 820
FatI CATG 5 cut(s) 667, 804, 912, 951, 1193
Fnu4HI GCNGC 9 cut(s) 171, 190, 322, 325, 340, 758, 1044, 1047, 1056
FokI GGATG 6 cut(s) 440, 496, 760, 824, 1003, 1172
Fsp4HI GCNGC 9 cut(s) 171, 190, 322, 325, 340, 758, 1044, 1047, 1056
FspI TGCGCA 1 cut(s) 975
GlaI GCGC 1 cut(s) 975
GluI GCNGC 9 cut(s) 171, 190, 322, 325, 340, 758, 1044, 1047, 1056
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 2 cut(s) 27, 1120
HapII CCGG 3 cut(s) 24, 221, 280
HhaI GCGC 1 cut(s) 976
Hin1II CATG 5 cut(s) 671, 808, 916, 955, 1197
Hin6I GCGC 1 cut(s) 974
HinP1I GCGC 1 cut(s) 974
HinfI GANTC 4 cut(s) 197, 410, 883, 956
HpaII CCGG 3 cut(s) 24, 221, 280
HphI GGTGA 6 cut(s) 461, 643, 698, 951, 993, 1054
Hpy188I TCNGA 4 cut(s) 268, 465, 500, 616
Hpy188III TCNNGA 6 cut(s) 88, 144, 306, 542, 1088, 1136
HpyAV CCTTC 5 cut(s) 19, 393, 523, 789, 1138
HpyCH4III ACNGT 4 cut(s) 248, 421, 996, 1142
HpyCH4V TGCA 4 cut(s) 327, 713, 760, 984
HpyF10VI GCNNNNNNNGC 3 cut(s) 1040, 1052, 1055
HpyF3I CTNAG 4 cut(s) 267, 462, 594, 1134
Hsp92II CATG 5 cut(s) 671, 808, 916, 955, 1197
HspAI GCGC 1 cut(s) 974
KpnI GGTACC 1 cut(s) 585
Kzo9I GATC 2 cut(s) 334, 1168
LmnI GCTCC 2 cut(s) 223, 347
Lsp1109I GCAGC 7 cut(s) 182, 201, 311, 333, 351, 744, 1033
LweI GCATC 1 cut(s) 744
MabI ACCWGGT 1 cut(s) 627
MaeIII GTNAC 8 cut(s) 248, 365, 388, 457, 469, 701, 779, 787
MalI GATC 2 cut(s) 336, 1170
MboI GATC 2 cut(s) 334, 1168
MboII GAAGA 8 cut(s) 499, 580, 616, 656, 738, 763, 870, 1178
MhlI GDGCHC 2 cut(s) 407, 539
MlyI GAGTC 2 cut(s) 191, 419
MmeI TCCRAC 1 cut(s) 677
MnlI CCTC 9 cut(s) 40, 424, 589, 660, 675, 682, 738, 869, 1009
MseI TTAA 4 cut(s) 275, 609, 930, 1231
MspA1I CMGCKG 2 cut(s) 324, 1046
MspI CCGG 3 cut(s) 24, 221, 280
MspR9I CCNGG 5 cut(s) 24, 280, 281, 629, 774
MvaI CCWGG 2 cut(s) 629, 774
MwoI GCNNNNNNNGC 3 cut(s) 1040, 1052, 1055
NciI CCSGG 3 cut(s) 24, 280, 281
NdeII GATC 2 cut(s) 334, 1168
NlaIII CATG 5 cut(s) 671, 808, 916, 955, 1197
NlaIV GGNNCC 2 cut(s) 28, 583
NmuCI GTSAC 5 cut(s) 365, 388, 457, 701, 779
NsbI TGCGCA 1 cut(s) 975
NspV TTCGAA 1 cut(s) 881
PaqCI CACCTGC 1 cut(s) 979
PfeI GAWTC 2 cut(s) 883, 956
PkrI GCNGC 9 cut(s) 172, 191, 323, 326, 341, 759, 1045, 1048, 1057
PleI GAGTC 2 cut(s) 191, 418
PpsI GAGTC 2 cut(s) 191, 418
Psp6I CCWGG 2 cut(s) 627, 772
PspGI CCWGG 2 cut(s) 627, 772
PspN4I GGNNCC 2 cut(s) 28, 583
PspPI GGNCC 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 324
RsaI GTAC 1 cut(s) 583
RsaNI GTAC 1 cut(s) 582
SaqAI TTAA 4 cut(s) 275, 609, 930, 1231
SatI GCNGC 9 cut(s) 171, 190, 322, 325, 340, 758, 1044, 1047, 1056
Sau3AI GATC 2 cut(s) 334, 1168
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 2 cut(s) 191, 419
ScrFI CCNGG 5 cut(s) 24, 280, 281, 629, 774
SduI GDGCHC 2 cut(s) 407, 539
SexAI ACCWGGT 1 cut(s) 627
SfaNI GCATC 1 cut(s) 744
SfcI CTRYAG 1 cut(s) 693
SfuI TTCGAA 1 cut(s) 881
SmaI CCCGGG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 540
SmoI CTYRAG 1 cut(s) 540
SsiI CCGC 3 cut(s) 579, 1044, 1056
StyD4I CCNGG 5 cut(s) 22, 278, 279, 627, 772
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 4 cut(s) 248, 421, 996, 1142
TaqI TCGA 1 cut(s) 881
TauI GCSGC 2 cut(s) 1046, 1058
TfiI GAWTC 2 cut(s) 883, 956
Tru1I TTAA 4 cut(s) 275, 609, 930, 1231
Tru9I TTAA 4 cut(s) 275, 609, 930, 1231
TscAI CASTG 5 cut(s) 870, 1036, 1045, 1057, 1147
TseFI GTSAC 5 cut(s) 365, 388, 457, 701, 779
TseI GCWGC 7 cut(s) 170, 189, 321, 324, 339, 757, 1046
Tsp45I GTSAC 5 cut(s) 365, 388, 457, 701, 779
TspDTI ATGAA 5 cut(s) 168, 656, 764, 828, 1175
TspMI CCCGGG 1 cut(s) 279
TspRI CASTG 5 cut(s) 870, 1036, 1045, 1057, 1147
XapI RAATTY 3 cut(s) 285, 494, 899
XmaI CCCGGG 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.