pycom14g13270

Protein ROOT INITIATION DEFECTIVE 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012617
Physical Location & Seq
Forward (+)
16229271 .. 16229954
684 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGCTCAAAACTTGGCGTGGACATGATAAATCTTTGAGCTGTATGCTGTTTTCGGATGATGGTTCTCTTCTTGTTTCTGGTTCAGATGATGGGATGATATTTGTGTGGAACTTGATTAGTTTGCTGGATGTAGAAAATGTTGAAAGCTTTCCGTCACCATTACATTATTCAACGGAGCATAAATCCTCTATAACTGGCTTGTTAACTACATCAGGCATCTCTAGTCCAGTGTTAATATCAAGCTCACTTGATGGCTTTTGCAAGGTTTGGGATCTAGTCTTGGGAAACCTCATGCATACTCAAGTTTATCCACCAGGGATAACTGCAGTTACTCTTCACTCAACGAAGCAGTTAATTTTCTCCGGAAGTATAGACGGAAGAATTTTTGTGAACAAACTCGAAATCGGACTGGTAGAGGATTATTTGGTTGGTGCTGAAGATCAGTCACCTGTGCTAAAAGGACACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0007368 GO:0007389 GO:0008150 GO:0009799 GO:0009855 GO:0032501 GO:0032502 GO:0048856 GO:0070121
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

157

Amino Acids

16.98

Weight (kDa)

5.16

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40 PF00400 2 - 37 4.3e-10 WD domain, G-beta repeat
WD40_Prp19 PF24814 2 - 99 4.5e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 3 - 151 2.5e-14 WDR5 beta-propeller domain
WDR55 PF24796 3 - 133 3.3e-07 WDR55
WD40_CDC20-Fz PF24807 3 - 121 3.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 4 - 130 1.4e-16 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 4 - 130 7.1e-09 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 8 - 128 1.8e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 362
AclWI GGATC 1 cut(s) 279
AcsI RAATTY 1 cut(s) 381
AcuI CTGAAG 1 cut(s) 456
AgsI TTSAA 2 cut(s) 143, 171
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 3 cut(s) 39, 147, 243
AluI AGCT 3 cut(s) 39, 147, 243
AlwI GGATC 1 cut(s) 279
Aor13HI TCCGGA 1 cut(s) 362
ApoI RAATTY 1 cut(s) 381
Asp700I GAANNNNTTC 1 cut(s) 147
AsuHPI GGTGA 2 cut(s) 147, 438
BccI CCATC 3 cut(s) 53, 83, 245
BciT130I CCWGG 1 cut(s) 315
BfaI CTAG 2 cut(s) 222, 275
BfmI CTRYAG 1 cut(s) 324
Bme1390I CCNGG 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 285
BsaJI CCNNGG 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 362
Bse1I ACTGG 3 cut(s) 199, 227, 414
BseAI TCCGGA 1 cut(s) 362
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 3 cut(s) 61, 99, 133
BseNI ACTGG 3 cut(s) 199, 227, 414
BsiSI CCGG 1 cut(s) 363
Bsp13I TCCGGA 1 cut(s) 362
Bsp143I GATC 2 cut(s) 271, 439
BspEI TCCGGA 1 cut(s) 362
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 1 cut(s) 279
BsrI ACTGG 3 cut(s) 199, 227, 414
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 2 cut(s) 271, 439
Bst2UI CCWGG 1 cut(s) 315
Bst6I CTCTTC 2 cut(s) 72, 339
BstF5I GGATG 3 cut(s) 61, 99, 133
BstKTI GATC 2 cut(s) 274, 442
BstMBI GATC 2 cut(s) 271, 439
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BtsCI GGATG 3 cut(s) 61, 99, 133
BtsIMutI CAGTG 1 cut(s) 234
CviAII CATG 2 cut(s) 23, 292
CviJI RGCY 5 cut(s) 39, 147, 198, 243, 255
CviKI_1 RGCY 5 cut(s) 39, 147, 198, 243, 255
DpnI GATC 2 cut(s) 273, 441
DpnII GATC 2 cut(s) 271, 439
Eam1104I CTCTTC 2 cut(s) 72, 339
EarI CTCTTC 2 cut(s) 72, 339
Eco57I CTGAAG 1 cut(s) 456
EcoRII CCWGG 1 cut(s) 313
EcoT22I ATGCAT 1 cut(s) 297
FaeI CATG 2 cut(s) 26, 295
FaiI YATR 7 cut(s) 24, 44, 180, 191, 293, 297, 371
FatI CATG 2 cut(s) 22, 291
FokI GGATG 3 cut(s) 68, 106, 140
FspBI CTAG 2 cut(s) 222, 275
HapII CCGG 1 cut(s) 363
Hin1II CATG 2 cut(s) 26, 295
HincII GTYRAC 1 cut(s) 204
HindII GTYRAC 1 cut(s) 204
HindIII AAGCTT 1 cut(s) 145
HpaI GTTAAC 1 cut(s) 204
HpaII CCGG 1 cut(s) 363
HphI GGTGA 2 cut(s) 147, 438
Hpy166II GTNNAC 3 cut(s) 20, 204, 391
Hpy188I TCNGA 3 cut(s) 55, 85, 407
Hpy188III TCNNGA 1 cut(s) 363
Hpy8I GTNNAC 3 cut(s) 20, 204, 391
HpyCH4V TGCA 3 cut(s) 261, 295, 326
Hsp92II CATG 2 cut(s) 26, 295
Kpn2I TCCGGA 1 cut(s) 362
KspAI GTTAAC 1 cut(s) 204
Kzo9I GATC 2 cut(s) 271, 439
LmnI GCTCC 1 cut(s) 175
LweI GCATC 1 cut(s) 225
MaeI CTAG 2 cut(s) 222, 275
MaeIII GTNAC 3 cut(s) 153, 328, 444
MalI GATC 2 cut(s) 273, 441
MboI GATC 2 cut(s) 271, 439
MboII GAAGA 4 cut(s) 59, 326, 390, 449
MflI RGATCY 1 cut(s) 271
MluCI AATT 2 cut(s) 354, 381
MnlI CCTC 3 cut(s) 196, 299, 409
Mph1103I ATGCAT 1 cut(s) 297
MroI TCCGGA 1 cut(s) 362
MroXI GAANNNNTTC 1 cut(s) 147
MseI TTAA 3 cut(s) 203, 233, 353
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
NdeII GATC 2 cut(s) 271, 439
NlaIII CATG 2 cut(s) 26, 295
NmuCI GTSAC 2 cut(s) 153, 444
NsiI ATGCAT 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 147
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PstI CTGCAG 1 cut(s) 328
PsuI RGATCY 1 cut(s) 271
SaqAI TTAA 3 cut(s) 203, 233, 353
Sau3AI GATC 2 cut(s) 271, 439
ScrFI CCNGG 1 cut(s) 315
SetI ASST 6 cut(s) 41, 149, 245, 267, 291, 451
SfaNI GCATC 1 cut(s) 225
SfcI CTRYAG 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
Sse9I AATT 2 cut(s) 354, 381
SspMI CTAG 2 cut(s) 222, 275
StyD4I CCNGG 1 cut(s) 313
TaqI TCGA 1 cut(s) 399
TasI AATT 2 cut(s) 354, 381
Tru1I TTAA 3 cut(s) 203, 233, 353
Tru9I TTAA 3 cut(s) 203, 233, 353
TscAI CASTG 1 cut(s) 234
TseFI GTSAC 2 cut(s) 153, 444
Tsp45I GTSAC 2 cut(s) 153, 444
TspGWI ACGGA 3 cut(s) 141, 188, 390
TspRI CASTG 1 cut(s) 234
XapI RAATTY 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 147
XspI CTAG 2 cut(s) 222, 275
Zsp2I ATGCAT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.