pycom14g15440

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004999
Physical Location & Seq
Reverse (-)
18060967 .. 18064949
3983 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1689 bp
ATGTCGCAGAAGAGGCAGCAGGATGATGGCAAGGGTCGATCGGATGGGACTAGTAGTCCGGATGACAAGCGTAGGAAGACCAGTTTCCAGAGCGTGGTTCTGGAAACGATGAGATTGCACAAAGTCCAGCAATTACTGGAGCCACTTCTAGAGCGTTTGATTCGTAAAGTTGTTAAAGAAGAAGTTGAGGTGGCCCTAAGAAAGCATTTGGCTAGTATGAAACAGAATTGTGGGAAAGAGACGGATCCTTCTGAATCAAGAATATTAAAGCTACAGTTCGTAAATAACATCTCTCTTCCAGTATTTACTGGAGCTCGGATTGAAGGAGAAGAATGCTCCACTGTACAAGTAGCTTTGGTCGATTGCTTTAGTGGACATATTGTCGAATCCGAGCCTGAATCCACAGCCAAGGTGGAAATAGTTGTTCTTGAGGGTGATTTTGATGGTGAGGAGGGTGATGATTGGACACTTGAAGATTTCAAGAATAACATTGTAAGAGAGAGGGATGGAAAGAAACCTCTTCTTACAGGAGAGGTAGTTTTGAATCTTAAAGACGGAGTTGCACCTCTGGGTGAGATTTCTTTTACTGATAATTCAAGCTGGACAAGGAGCCGTAAGTTCAGGCTAGGAGCAAGAGTTTCAGACAACTTCGATGGAACTAGAATAAGAGAAGCAAGGACAGAATCCTTTATTGTTAGGGATCATCGGGGAGAATTGTACAAGAAACACCACCCTGCATCTCTGCTTGATGAAGTATGGAGACTAGAAAAGATTGGCAAGGACGGAGCTTTCCATAAGCGGTTGAGTCGGGAAAACATCAACACTGTGAATGATTTTCTCATCCAACTCTTCATAAATCCTTCAAGGCTCCGGAATATCCTTGGGACGGGTATGTCTGCTAAGATGTGGGAAGTCACTGTGGAGCATGCTCAGACATGTACACTCGATAAGAGGGTGTACTTGTACTGCCCTCCCGGTTCTCAGGAAACTGGTGTGGCCTTCAACATTGTAGGGCAAGTGATGGGACTTCTGTTGGAACATGAATATGTTTCTGTTAGTAAGCTGTCTGAAACACAAAAGGCTGATGGCCACCACCTGGTCATTTCTGCATTTGACCACTGGGAAGAAGTAGTCCTGTTTGAAGATGAAGCCTCTCTTATAGCTGCATCTTCCAACTTCACTAATGTTCTTCACACATCGAGCTCACCAAGGGCAGAGGATCTTGACGGAAGCAAGTTTTTGGCTTCACAAAAGATTGGTGGATTTGATTATGCACAGCCATCTGCCTCTTCTCCAGATATCATCTCATCCATCTATTCCGTGGGTAGCACCAGTGGCTTGGATGATTATGCCTTGCACAACATTGACGGCTTCGGTCTTAGATATGATCAGCCTCTGAGTTTCCCAGGTCAAGTCACCAACTCCCTGATCTTTGACACCGACCCCATGGCTCATGCTTTATTTGTTGAGGATCATCTGCAGTTTTCCGATGCTGATCTTCAGTCCCAGAACATGAGTTCAGAACCACAAGATCTACATAGCGCTGTGGATCGCTTCTTGCCGAGTTCAGCTGTTGCCATATCTGATAAGGCTCAGAGGGGATGGACAAAGCTATTCAGTGTGCTGCAGTGGTTCTCAATATGGAGGATCGTGGCTTTGAGACGAAGAGTTCAAGATATTCAGAGATAG

Protein Analysis

563

Amino Acids

63.17

Weight (kDa)

5.53

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 89 - 235 5e-59 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 248 - 313 7.8e-32 Calmodulin binding protein central domain
Calmod_bind_C PF20452 319 - 378 9.3e-16 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 892
AccB7I CCANNNNNTGG 2 cut(s) 94, 1096
AccIII TCCGGA 2 cut(s) 58, 870
AciI CCGC 1 cut(s) 799
AclWI GGATC 7 cut(s) 239, 252, 708, 1227, 1479, 1557, 1655
AcoI YGGCCR 1 cut(s) 1087
AcuI CTGAAG 1 cut(s) 1484
AfaI GTAC 5 cut(s) 345, 719, 940, 959, 965
AfeI AGCGCT 1 cut(s) 1543
AfiI CCNNNNNNNGG 3 cut(s) 94, 886, 1096
AflIII ACRYGT 1 cut(s) 935
AgsI TTSAA 9 cut(s) 323, 473, 481, 544, 597, 864, 1003, 1142, 1673
AhdI GACNNNNNGTC 2 cut(s) 54, 380
AhlI ACTAGT 1 cut(s) 50
AjnI CCWGG 2 cut(s) 1095, 1405
Alw21I GWGCWC 2 cut(s) 316, 1205
Alw26I GTCTC 3 cut(s) 233, 754, 1654
AlwI GGATC 7 cut(s) 239, 252, 708, 1227, 1479, 1557, 1655
AlwNI CAGNNNCTG 1 cut(s) 1396
Aor13HI TCCGGA 2 cut(s) 58, 870
Aor51HI AGCGCT 1 cut(s) 1543
AoxI GGCC 3 cut(s) 192, 996, 1087
ApeKI GCWGC 3 cut(s) 16, 1163, 1624
AspLEI GCGC 1 cut(s) 1544
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 975
AsuHPI GGTGA 6 cut(s) 446, 458, 467, 584, 1197, 1408
BalI TGGCCA 1 cut(s) 1089
BamHI GGATCC 1 cut(s) 244
BanII GRGCYC 2 cut(s) 316, 1205
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 2 cut(s) 316, 1205
BbvI GCAGC 3 cut(s) 28, 1150, 1611
BceAI ACGGC 2 cut(s) 597, 1384
BcgI CGANNNNNNTGC 2 cut(s) 97, 131
BciT130I CCWGG 2 cut(s) 1097, 1407
BclI TGATCA 1 cut(s) 1387
BcnI CCSGG 1 cut(s) 975
BcoDI GTCTC 3 cut(s) 233, 754, 1654
BcuI ACTAGT 1 cut(s) 50
BfaI CTAG 6 cut(s) 51, 149, 213, 626, 660, 764
BfmI CTRYAG 3 cut(s) 272, 1478, 1625
BfoI RGCGCY 1 cut(s) 1545
BglII AGATCT 1 cut(s) 1531
BisI GCNGC 3 cut(s) 17, 1164, 1625
BlsI GCNGC 3 cut(s) 18, 1165, 1626
Bme1390I CCNGG 3 cut(s) 975, 1097, 1407
BmeRI GACNNNNNGTC 2 cut(s) 54, 380
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 4 cut(s) 141, 246, 611, 869
BmrFI CCNGG 3 cut(s) 975, 1097, 1407
BmrI ACTGGG 1 cut(s) 1129
BmsI GCATC 3 cut(s) 746, 1175, 1480
BmuI ACTGGG 1 cut(s) 1129
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 3 cut(s) 158, 330, 1278
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 975
BsaBI GATNNNNATC 1 cut(s) 1494
BsaJI CCNNGG 6 cut(s) 408, 880, 1208, 1320, 1405, 1446
BsaWI WCCGGW 2 cut(s) 58, 870
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 621, 651
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 886, 1096
Bse1I ACTGG 7 cut(s) 81, 141, 299, 313, 994, 1124, 1332
Bse8I GATNNNNATC 1 cut(s) 1494
BseAI TCCGGA 2 cut(s) 58, 870
BseBI CCWGG 2 cut(s) 1097, 1407
BseDI CCNNGG 6 cut(s) 408, 880, 1208, 1320, 1405, 1446
BseGI GGATG 8 cut(s) 28, 49, 67, 511, 840, 1307, 1348, 1607
BseJI GATNNNNATC 1 cut(s) 1494
BseLI CCNNNNNNNGG 3 cut(s) 94, 886, 1096
BseMII CTCAG 4 cut(s) 944, 995, 1388, 1607
BseNI ACTGG 7 cut(s) 81, 141, 299, 313, 994, 1124, 1332
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 3 cut(s) 28, 1150, 1611
Bsh1285I CGRYCG 1 cut(s) 41
BshFI GGCC 3 cut(s) 194, 998, 1089
BsiEI CGRYCG 1 cut(s) 41
BsiHKAI GWGCWC 2 cut(s) 316, 1205
BsiSI CCGG 3 cut(s) 59, 871, 975
BslFI GGGAC 4 cut(s) 61, 898, 1038, 1489
BslI CCNNNNNNNGG 3 cut(s) 94, 886, 1096
BsmAI GTCTC 3 cut(s) 233, 754, 1654
BsmBI CGTCTC 2 cut(s) 233, 1654
BsmFI GGGAC 4 cut(s) 61, 898, 1038, 1489
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 3 cut(s) 194, 998, 1089
Bsp1286I GDGCHC 2 cut(s) 316, 1205
Bsp13I TCCGGA 2 cut(s) 58, 870
Bsp1407I TGTACA 3 cut(s) 343, 717, 938
Bsp19I CCATGG 1 cut(s) 1446
BspACI CCGC 1 cut(s) 799
BspANI GGCC 3 cut(s) 194, 998, 1089
BspCNI CTCAG 4 cut(s) 943, 994, 1389, 1606
BspEI TCCGGA 2 cut(s) 58, 870
BspLI GGNNCC 4 cut(s) 141, 246, 611, 869
BspMAI CTGCAG 2 cut(s) 1482, 1629
BspPI GGATC 7 cut(s) 239, 252, 708, 1227, 1479, 1557, 1655
BsrGI TGTACA 3 cut(s) 343, 717, 938
BsrI ACTGG 7 cut(s) 81, 141, 299, 313, 994, 1124, 1332
BssECI CCNNGG 6 cut(s) 408, 880, 1208, 1320, 1405, 1446
BssT1I CCWWGG 4 cut(s) 408, 880, 1208, 1446
Bst2UI CCWGG 2 cut(s) 1097, 1407
Bst4CI ACNGT 4 cut(s) 276, 343, 826, 919
Bst6I CTCTTC 6 cut(s) 5, 300, 525, 854, 1294, 1660
BstAUI TGTACA 3 cut(s) 343, 717, 938
BstC8I GCNNGC 1 cut(s) 927
BstDEI CTNAG 7 cut(s) 197, 900, 930, 981, 1379, 1397, 1593
BstDSI CCRYGG 2 cut(s) 1320, 1446
BstF5I GGATG 8 cut(s) 28, 49, 67, 511, 840, 1307, 1348, 1607
BstH2I RGCGCY 1 cut(s) 1545
BstHHI GCGC 1 cut(s) 1544
BstMAI GTCTC 3 cut(s) 233, 754, 1654
BstMCI CGRYCG 1 cut(s) 41
BstMWI GCNNNNNNNGC 2 cut(s) 13, 1335
BstNI CCWGG 2 cut(s) 1097, 1407
BstNSI RCATGY 2 cut(s) 929, 939
BstSCI CCNGG 3 cut(s) 973, 1095, 1405
BstSFI CTRYAG 3 cut(s) 272, 1478, 1625
BstV1I GCAGC 3 cut(s) 28, 1150, 1611
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 3 cut(s) 244, 1219, 1531
BstXI CCANNNNNNTGG 1 cut(s) 1339
BstYI RGATCY 3 cut(s) 244, 1219, 1531
BsuRI GGCC 3 cut(s) 194, 998, 1089
BtgI CCRYGG 2 cut(s) 1320, 1446
BtsCI GGATG 8 cut(s) 28, 49, 67, 511, 840, 1307, 1348, 1607
BtsI GCAGTG 1 cut(s) 1634
BtsIMutI CAGTG 7 cut(s) 339, 822, 915, 1117, 1339, 1624, 1634
Cac8I GCNNGC 1 cut(s) 927
CaiI CAGNNNCTG 1 cut(s) 1396
CfoI GCGC 1 cut(s) 1544
Cfr13I GGNCC 1 cut(s) 193
CsiI ACCWGGT 1 cut(s) 1095
Csp6I GTAC 5 cut(s) 344, 718, 939, 958, 964
CviAII CATG 6 cut(s) 926, 936, 1040, 1447, 1454, 1513
CviQI GTAC 5 cut(s) 344, 718, 939, 958, 964
DdeI CTNAG 7 cut(s) 197, 900, 930, 981, 1379, 1397, 1593
DrdI GACNNNNNNGTC 1 cut(s) 892
DriI GACNNNNNGTC 2 cut(s) 54, 380
DseDI GACNNNNNNGTC 1 cut(s) 892
EaeI YGGCCR 1 cut(s) 1087
Eam1104I CTCTTC 6 cut(s) 5, 300, 525, 854, 1294, 1660
Eam1105I GACNNNNNGTC 2 cut(s) 54, 380
EarI CTCTTC 6 cut(s) 5, 300, 525, 854, 1294, 1660
Ecl136II GAGCTC 2 cut(s) 314, 1203
Eco130I CCWWGG 4 cut(s) 408, 880, 1208, 1446
Eco24I GRGCYC 2 cut(s) 316, 1205
Eco32I GATATC 1 cut(s) 1300
Eco47III AGCGCT 1 cut(s) 1543
Eco53kI GAGCTC 2 cut(s) 314, 1203
Eco57I CTGAAG 1 cut(s) 1484
EcoICRI GAGCTC 2 cut(s) 314, 1203
EcoRII CCWGG 2 cut(s) 1095, 1405
EcoRV GATATC 1 cut(s) 1300
EcoT14I CCWWGG 4 cut(s) 408, 880, 1208, 1446
EcoT38I GRGCYC 2 cut(s) 316, 1205
ErhI CCWWGG 4 cut(s) 408, 880, 1208, 1446
Esp3I CGTCTC 2 cut(s) 233, 1654
FaeI CATG 6 cut(s) 929, 939, 1043, 1450, 1457, 1516
FalI AAGNNNNNCTT 2 cut(s) 1140, 1172
FaqI GGGAC 4 cut(s) 61, 898, 1038, 1489
FatI CATG 6 cut(s) 925, 935, 1039, 1446, 1453, 1512
FbaI TGATCA 1 cut(s) 1387
Fnu4HI GCNGC 3 cut(s) 17, 1164, 1625
FokI GGATG 8 cut(s) 35, 56, 74, 518, 827, 1294, 1355, 1614
FriOI GRGCYC 2 cut(s) 316, 1205
Fsp4HI GCNGC 3 cut(s) 17, 1164, 1625
FspBI CTAG 6 cut(s) 51, 149, 213, 626, 660, 764
GlaI GCGC 1 cut(s) 1543
GluI GCNGC 3 cut(s) 17, 1164, 1625
GsuI CTGGAG 3 cut(s) 158, 330, 1278
HaeII RGCGCY 1 cut(s) 1545
HaeIII GGCC 3 cut(s) 194, 998, 1089
HapII CCGG 3 cut(s) 59, 871, 975
HhaI GCGC 1 cut(s) 1544
Hin1II CATG 6 cut(s) 929, 939, 1043, 1450, 1457, 1516
Hin6I GCGC 1 cut(s) 1542
HinP1I GCGC 1 cut(s) 1542
HinfI GANTC 7 cut(s) 160, 254, 386, 398, 544, 683, 805
HpaII CCGG 3 cut(s) 59, 871, 975
HphI GGTGA 6 cut(s) 446, 458, 467, 584, 1197, 1408
Hpy166II GTNNAC 3 cut(s) 374, 941, 958
Hpy8I GTNNAC 3 cut(s) 374, 941, 958
HpyAV CCTTC 4 cut(s) 258, 317, 870, 1009
HpyCH4III ACNGT 4 cut(s) 276, 343, 826, 919
HpyCH4V TGCA 9 cut(s) 118, 563, 737, 1109, 1166, 1274, 1357, 1480, 1627
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 1335
HpyF3I CTNAG 7 cut(s) 197, 900, 930, 981, 1379, 1397, 1593
Hsp92II CATG 6 cut(s) 929, 939, 1043, 1450, 1457, 1516
HspAI GCGC 1 cut(s) 1542
Kpn2I TCCGGA 2 cut(s) 58, 870
Ksp22I TGATCA 1 cut(s) 1387
LmnI GCTCC 8 cut(s) 139, 311, 341, 609, 629, 785, 873, 922
Lsp1109I GCAGC 3 cut(s) 28, 1150, 1611
LweI GCATC 3 cut(s) 746, 1175, 1480
MabI ACCWGGT 1 cut(s) 1095
MaeI CTAG 6 cut(s) 51, 149, 213, 626, 660, 764
MaeIII GTNAC 2 cut(s) 913, 1414
MflI RGATCY 3 cut(s) 244, 1219, 1531
MhlI GDGCHC 2 cut(s) 316, 1205
MlsI TGGCCA 1 cut(s) 1089
MluCI AATT 4 cut(s) 131, 226, 592, 713
MluNI TGGCCA 1 cut(s) 1089
MlyI GAGTC 1 cut(s) 814
MmeI TCCRAC 3 cut(s) 868, 1014, 1197
Mox20I TGGCCA 1 cut(s) 1089
MroI TCCGGA 2 cut(s) 58, 870
MscI TGGCCA 1 cut(s) 1089
MseI TTAA 3 cut(s) 174, 266, 549
MslI CAYNNNNRTG 1 cut(s) 1044
Msp20I TGGCCA 1 cut(s) 1089
MspA1I CMGCKG 1 cut(s) 1571
MspI CCGG 3 cut(s) 59, 871, 975
MspR9I CCNGG 3 cut(s) 975, 1097, 1407
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 2 cut(s) 1097, 1407
MwoI GCNNNNNNNGC 2 cut(s) 13, 1335
NciI CCSGG 1 cut(s) 975
NcoI CCATGG 1 cut(s) 1446
NlaIII CATG 6 cut(s) 929, 939, 1043, 1450, 1457, 1516
NlaIV GGNNCC 4 cut(s) 141, 246, 611, 869
NmeAIII GCCGAG 1 cut(s) 1587
NmuCI GTSAC 2 cut(s) 913, 1414
NspI RCATGY 2 cut(s) 929, 939
PaeI GCATGC 1 cut(s) 929
PciI ACATGT 1 cut(s) 935
PctI GAATGC 1 cut(s) 338
PfeI GAWTC 6 cut(s) 160, 254, 386, 398, 544, 683
PflMI CCANNNNNTGG 2 cut(s) 94, 1096
PkrI GCNGC 3 cut(s) 18, 1165, 1626
Ple19I CGATCG 1 cut(s) 41
PleI GAGTC 1 cut(s) 813
PpsI GAGTC 1 cut(s) 813
PscI ACATGT 1 cut(s) 935
Psp124BI GAGCTC 2 cut(s) 316, 1205
Psp6I CCWGG 2 cut(s) 1095, 1405
PspGI CCWGG 2 cut(s) 1095, 1405
PspN4I GGNNCC 4 cut(s) 141, 246, 611, 869
PspPI GGNCC 1 cut(s) 193
PstI CTGCAG 2 cut(s) 1482, 1629
PstNI CAGNNNCTG 1 cut(s) 1396
PsuI RGATCY 3 cut(s) 244, 1219, 1531
PvuI CGATCG 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 1571
RsaI GTAC 5 cut(s) 345, 719, 940, 959, 965
RsaNI GTAC 5 cut(s) 344, 718, 939, 958, 964
RseI CAYNNNNRTG 1 cut(s) 1044
SacI GAGCTC 2 cut(s) 316, 1205
SaqAI TTAA 3 cut(s) 174, 266, 549
SatI GCNGC 3 cut(s) 17, 1164, 1625
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 1 cut(s) 814
ScrFI CCNGG 3 cut(s) 975, 1097, 1407
SduI GDGCHC 2 cut(s) 316, 1205
SexAI ACCWGGT 1 cut(s) 1095
SfaNI GCATC 3 cut(s) 746, 1175, 1480
SfcI CTRYAG 3 cut(s) 272, 1478, 1625
SmiMI CAYNNNNRTG 1 cut(s) 1044
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SpeI ACTAGT 1 cut(s) 50
SphI GCATGC 1 cut(s) 929
Sse9I AATT 4 cut(s) 131, 226, 592, 713
SsiI CCGC 1 cut(s) 799
SspI AATATT 1 cut(s) 264
SspMI CTAG 6 cut(s) 51, 149, 213, 626, 660, 764
SstI GAGCTC 2 cut(s) 316, 1205
StyD4I CCNGG 3 cut(s) 973, 1095, 1405
StyI CCWWGG 4 cut(s) 408, 880, 1208, 1446
TaaI ACNGT 4 cut(s) 276, 343, 826, 919
TaqI TCGA 6 cut(s) 37, 360, 384, 651, 945, 1199
TaqII GACCGA 1 cut(s) 1364
TasI AATT 4 cut(s) 131, 226, 592, 713
TatI WGTACW 5 cut(s) 343, 717, 938, 957, 963
TfiI GAWTC 6 cut(s) 160, 254, 386, 398, 544, 683
Tru1I TTAA 3 cut(s) 174, 266, 549
Tru9I TTAA 3 cut(s) 174, 266, 549
TscAI CASTG 7 cut(s) 346, 829, 922, 1124, 1339, 1624, 1634
TseFI GTSAC 2 cut(s) 913, 1414
TseI GCWGC 3 cut(s) 16, 1163, 1624
Tsp45I GTSAC 2 cut(s) 913, 1414
TspDTI ATGAA 5 cut(s) 233, 765, 841, 1056, 1161
TspGWI ACGGA 5 cut(s) 257, 570, 798, 1242, 1309
TspRI CASTG 7 cut(s) 346, 829, 922, 1124, 1339, 1624, 1634
Van91I CCANNNNNTGG 2 cut(s) 94, 1096
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 2 cut(s) 929, 939
XcmI CCANNNNNNNNNTGG 2 cut(s) 409, 1318
XspI CTAG 6 cut(s) 51, 149, 213, 626, 660, 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.