pycom14g16180

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0014742
Physical Location & Seq
Reverse (-)
18559674 .. 18560835
1162 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 732 bp
ATGAGTAACACTACGGGTGTGCGCTACAACATTGGCAAGGATGCCAGAGATCTTCTCTACAAGGACTATGTTCATGAACCGCCGATTCGGTTTGACTCCAAAAACTTCAACTGGATCAGTGACATCTCGTGCAAAGTTAATTTGATTGCTCCTGGACTCAGCACCCTTTTCAGTATTAACGTGCCAGATTATGGCCGGGTAGAAATGCAATACGAAAATGGTGTTGTCGAGATTGCTGGAGGAATCGGGTTGAAAAGAAATCCGCTGAAAATGTGTGATCCGATTGCACTCTTTTCTGCAGTGATAGGAGGCAGTTCTGTGTGCTCCATTGGAACCGACCTTGCCTTCGACATATCTGCGGGAGAATTCGCAAAATTCAGTGCCGGTTTCAGCTTGAACAGCGACTTTGTTTCTGCTTCCTTATCCATGGACAAGCTTGACACTTTAAAAGCTTCATGCTTCACCGTGGTCAACCCCCTGAGCAACACAGCCCTTGGAGCAGAGTTGAAGCACCGGTTTTCAACCAACGATAGTGCCCTTGCTGTTGGCGCCCAGCACGCGTTTTTTCCATCTACCCTGGCGAAGGCTCGAGTTAGCACATACGGGAATGTTGGCGTTCTTATACGGCAAGGGTTATCGCAGAAGTTGTTCCTGTCTATGTCCGGAGAAGTGGATTTTATGAGCGTACAGAGAAGTCCAAGATTTGGATTCTCTCTTTCTGTTAGGCTCTGA

Protein Analysis

244

Amino Acids

26.25

Weight (kDa)

8.32

Isoelectric Point (pI)

24.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 47 - 236 5.5e-29 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014742)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15480
malus_domestica MD06G1188300.v1.1 MD14G1194800.v1.1
prunus_persica Prupe.5G186500_v2.0.a1
pyrus_communis pycom14g16180
rosa_chinensis RchiOBHm_Chr7g0180611
rosa_laevigata RLG00000005250
rosa_multiflora Rmu_sc0005690.1_g000021 Rmu_sc0023372.1_g000001 Rmu_sc0023372.1_g000002
rosa_roxburghii Rroxscaffold_3G00272740
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035600 Rh7BG035500 Rh7CG037200 Rh7DG035800
rosa_wichuraiana Rw7G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 548
AccB7I CCANNNNNTGG 2 cut(s) 191, 704
AccII CGCG 1 cut(s) 560
AccIII TCCGGA 1 cut(s) 662
AciI CCGC 3 cut(s) 80, 263, 359
AclWI GGATC 2 cut(s) 122, 272
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 2 cut(s) 365, 374
AcyI GRCGYC 1 cut(s) 549
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 3 cut(s) 191, 583, 704
AflIII ACRYGT 1 cut(s) 558
AgeI ACCGGT 1 cut(s) 513
AgsI TTSAA 5 cut(s) 109, 253, 397, 508, 522
AjnI CCWGG 2 cut(s) 151, 576
AluBI AGCT 3 cut(s) 393, 436, 452
AluI AGCT 3 cut(s) 393, 436, 452
Alw21I GWGCWC 1 cut(s) 326
AlwI GGATC 2 cut(s) 122, 272
Ama87I CYCGRG 1 cut(s) 588
Aor13HI TCCGGA 1 cut(s) 662
AoxI GGCC 1 cut(s) 193
ApoI RAATTY 2 cut(s) 365, 374
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AsiGI ACCGGT 1 cut(s) 513
Asp700I GAANNNNTTC 1 cut(s) 647
AspLEI GCGC 2 cut(s) 24, 551
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 454
AvaI CYCGRG 1 cut(s) 588
BaeGI GKGCMC 1 cut(s) 538
BanI GGYRCC 1 cut(s) 548
BauI CACGAG 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 326
BccI CCATC 1 cut(s) 577
BceAI ACGGC 1 cut(s) 641
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 2 cut(s) 153, 578
BcnI CCSGG 1 cut(s) 197
BfmI CTRYAG 1 cut(s) 297
BfoI RGCGCY 1 cut(s) 552
BglII AGATCT 1 cut(s) 49
Bme1390I CCNGG 3 cut(s) 153, 197, 578
BmeT110I CYCGRG 1 cut(s) 588
BmiI GGNNCC 2 cut(s) 334, 550
BmrFI CCNGG 3 cut(s) 153, 197, 578
BmsI GCATC 1 cut(s) 31
BplI GAGNNNNNCTC 2 cut(s) 39, 71
BpmI CTGGAG 1 cut(s) 258
Bpu10I CCTNAGC 1 cut(s) 479
BpuMI CCSGG 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 549
BsaJI CCNNGG 4 cut(s) 426, 465, 493, 576
BsaWI WCCGGW 2 cut(s) 513, 662
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 583, 704
Bse118I RCCGGY 2 cut(s) 383, 513
Bse1I ACTGG 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 662
BseBI CCWGG 2 cut(s) 153, 578
BseDI CCNNGG 4 cut(s) 426, 465, 493, 576
BseGI GGATG 1 cut(s) 46
BseLI CCNNNNNNNGG 3 cut(s) 191, 583, 704
BseMII CTCAG 2 cut(s) 172, 470
BseNI ACTGG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 538
BseYI CCCAGC 1 cut(s) 552
Bsh1236I CGCG 1 cut(s) 560
BshFI GGCC 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 548
BshTI ACCGGT 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 4 cut(s) 196, 384, 514, 663
BslI CCNNNNNNNGG 3 cut(s) 191, 583, 704
BsnI GGCC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 588
Bsp1286I GDGCHC 2 cut(s) 326, 538
Bsp13I TCCGGA 1 cut(s) 662
Bsp143I GATC 3 cut(s) 49, 114, 277
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 3 cut(s) 80, 263, 359
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 2 cut(s) 171, 471
BspEI TCCGGA 1 cut(s) 662
BspFNI CGCG 1 cut(s) 560
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 2 cut(s) 334, 550
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 2 cut(s) 122, 272
BspT107I GGYRCC 1 cut(s) 548
BsrFI RCCGGY 2 cut(s) 383, 513
BsrI ACTGG 1 cut(s) 116
BssAI RCCGGY 2 cut(s) 383, 513
BssECI CCNNGG 4 cut(s) 426, 465, 493, 576
BssMI GATC 3 cut(s) 49, 114, 277
BssNI GRCGYC 1 cut(s) 549
BssSI CACGAG 1 cut(s) 127
BssT1I CCWWGG 2 cut(s) 426, 493
Bst2BI CACGAG 1 cut(s) 127
Bst2UI CCWGG 2 cut(s) 153, 578
Bst4CI ACNGT 1 cut(s) 466
BstACI GRCGYC 1 cut(s) 549
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 2 cut(s) 158, 479
BstDSI CCRYGG 2 cut(s) 426, 465
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 1 cut(s) 46
BstFNI CGCG 1 cut(s) 560
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 2 cut(s) 24, 551
BstKTI GATC 3 cut(s) 52, 117, 280
BstMBI GATC 3 cut(s) 49, 114, 277
BstMWI GCNNNNNNNGC 4 cut(s) 399, 497, 548, 557
BstNI CCWGG 2 cut(s) 153, 578
BstSCI CCNGG 3 cut(s) 151, 195, 576
BstSFI CTRYAG 1 cut(s) 297
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 1 cut(s) 560
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 195
BtgI CCRYGG 2 cut(s) 426, 465
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 3 cut(s) 124, 306, 385
Cac8I GCNNGC 1 cut(s) 558
CciI TCATGA 1 cut(s) 73
CfoI GCGC 2 cut(s) 24, 551
Cfr10I RCCGGY 2 cut(s) 383, 513
Csp6I GTAC 1 cut(s) 686
CspAI ACCGGT 1 cut(s) 513
CviAII CATG 3 cut(s) 74, 427, 456
CviJI RGCY 7 cut(s) 195, 393, 436, 452, 491, 587, 727
CviKI_1 RGCY 7 cut(s) 195, 393, 436, 452, 491, 587, 727
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 2 cut(s) 158, 479
DinI GGCGCC 1 cut(s) 550
DpnI GATC 3 cut(s) 51, 116, 279
DpnII GATC 3 cut(s) 49, 114, 277
DraI TTTAAA 1 cut(s) 447
EaeI YGGCCR 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 426, 493
Eco88I CYCGRG 1 cut(s) 588
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoRI GAATTC 1 cut(s) 365
EcoRII CCWGG 2 cut(s) 151, 576
EcoT14I CCWWGG 2 cut(s) 426, 493
EgeI GGCGCC 1 cut(s) 550
EheI GGCGCC 1 cut(s) 550
ErhI CCWWGG 2 cut(s) 426, 493
FaeI CATG 3 cut(s) 77, 430, 459
FatI CATG 3 cut(s) 73, 426, 455
FauI CCCGC 1 cut(s) 352
FokI GGATG 1 cut(s) 53
GlaI GCGC 2 cut(s) 23, 550
GsaI CCCAGC 1 cut(s) 556
GsuI CTGGAG 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 1 cut(s) 195
HapII CCGG 4 cut(s) 196, 384, 514, 663
HhaI GCGC 2 cut(s) 24, 551
Hin1I GRCGYC 1 cut(s) 549
Hin1II CATG 3 cut(s) 77, 430, 459
Hin6I GCGC 2 cut(s) 22, 549
HinP1I GCGC 2 cut(s) 22, 549
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 2 cut(s) 434, 450
HinfI GANTC 5 cut(s) 85, 95, 156, 243, 708
HpaII CCGG 4 cut(s) 196, 384, 514, 663
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 2 cut(s) 282, 731
Hpy188III TCNNGA 3 cut(s) 74, 229, 663
Hpy8I GTNNAC 1 cut(s) 472
HpyAV CCTTC 2 cut(s) 355, 577
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 4 cut(s) 132, 208, 287, 299
HpyF10VI GCNNNNNNNGC 4 cut(s) 399, 497, 548, 557
HpyF3I CTNAG 2 cut(s) 158, 479
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 549
Hsp92II CATG 3 cut(s) 77, 430, 459
HspAI GCGC 2 cut(s) 22, 549
KasI GGCGCC 1 cut(s) 548
Kpn2I TCCGGA 1 cut(s) 662
Kzo9I GATC 3 cut(s) 49, 114, 277
LmnI GCTCC 3 cut(s) 154, 329, 497
LweI GCATC 1 cut(s) 31
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 5, 119
MalI GATC 3 cut(s) 51, 116, 279
MboI GATC 3 cut(s) 49, 114, 277
MboII GAAGA 1 cut(s) 44
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 2 cut(s) 326, 538
MluCI AATT 3 cut(s) 139, 365, 374
MluI ACGCGT 1 cut(s) 558
Mly113I GGCGCC 1 cut(s) 549
MlyI GAGTC 2 cut(s) 89, 150
MnlI CCTC 2 cut(s) 233, 302
MroI TCCGGA 1 cut(s) 662
MroXI GAANNNNTTC 1 cut(s) 647
MseI TTAA 3 cut(s) 138, 177, 446
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 4 cut(s) 196, 384, 514, 663
MspR9I CCNGG 3 cut(s) 153, 197, 578
MvaI CCWGG 2 cut(s) 153, 578
MvnI CGCG 1 cut(s) 560
MwoI GCNNNNNNNGC 4 cut(s) 399, 497, 548, 557
NarI GGCGCC 1 cut(s) 549
NciI CCSGG 1 cut(s) 197
NcoI CCATGG 1 cut(s) 426
NdeII GATC 3 cut(s) 49, 114, 277
NlaIII CATG 3 cut(s) 77, 430, 459
NlaIV GGNNCC 2 cut(s) 334, 550
NmuCI GTSAC 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 588
PagI TCATGA 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 3 cut(s) 85, 243, 708
PflMI CCANNNNNTGG 2 cut(s) 191, 704
PfoI TCCNGGA 1 cut(s) 151
PinAI ACCGGT 1 cut(s) 513
PleI GAGTC 2 cut(s) 89, 150
PluTI GGCGCC 1 cut(s) 552
PpsI GAGTC 2 cut(s) 89, 150
Psp6I CCWGG 2 cut(s) 151, 576
PspFI CCCAGC 1 cut(s) 552
PspGI CCWGG 2 cut(s) 151, 576
PspN4I GGNNCC 2 cut(s) 334, 550
PspXI VCTCGAGB 1 cut(s) 588
PstI CTGCAG 1 cut(s) 301
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
SaqAI TTAA 3 cut(s) 138, 177, 446
Sau3AI GATC 3 cut(s) 49, 114, 277
SchI GAGTC 2 cut(s) 89, 150
ScrFI CCNGG 3 cut(s) 153, 197, 578
SduI GDGCHC 2 cut(s) 326, 538
SetI ASST 5 cut(s) 183, 342, 395, 438, 454
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 297
SfoI GGCGCC 1 cut(s) 550
Sfr274I CTCGAG 1 cut(s) 588
SlaI CTCGAG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
Sse9I AATT 3 cut(s) 139, 365, 374
SsiI CCGC 3 cut(s) 80, 263, 359
SspDI GGCGCC 1 cut(s) 548
StyD4I CCNGG 3 cut(s) 151, 195, 576
StyI CCWWGG 2 cut(s) 426, 493
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 1 cut(s) 183
TaqI TCGA 3 cut(s) 228, 348, 589
TasI AATT 3 cut(s) 139, 365, 374
TfiI GAWTC 3 cut(s) 85, 243, 708
Tru1I TTAA 3 cut(s) 138, 177, 446
Tru9I TTAA 3 cut(s) 138, 177, 446
TscAI CASTG 3 cut(s) 124, 306, 385
TseFI GTSAC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 3 cut(s) 62, 90, 444
TspRI CASTG 3 cut(s) 124, 306, 385
Van91I CCANNNNNTGG 2 cut(s) 191, 704
XagI CCTNNNNNAGG 1 cut(s) 581
XapI RAATTY 2 cut(s) 365, 374
XhoI CTCGAG 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.