pycom14g17110

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011359
Physical Location & Seq
Reverse (-)
19179552 .. 19182740
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1386 bp
ATGGCTTCTGAAAATTACCAATCCAAAAATGTATGCGTGATTGGAGCTGGACCCTCAGGGCTTGTAGCTGCAAGGGAGCTGAGGAACGAAGGTCACAGGGTGGTGGTGCTGGAACAAAACCATGATGTGGGAGGGCAGTGGCTGTACGACCCAAATGTGGAGGGAGAGGATCCCCTAGGAAGGGCACCTACCCTGAAAGTGCATAGCAGTCTTTACACGTCTTTGAGACTCATGTTTCCAAGAGAGATCACGGGGTTCACTGACTTTCCATTTGCAGTCGAGAAAGGTAGGGACATGAGGAGGTTTCCTGGCCATACGGAGCTTCTTTTGTACCTCAAGGATTTTTGTGATTGGTTTGGGTTGAGGGAGTTAATAAGGTTCAATACGAGGGTTTCATATGTGGGGATGTTGGATGGAGATCATGCAGTTGGTTGCAAAGATTTGAAATGGGTTGTGAGGAGTGTGGAGAAGAAGACCGAGATTTTCGTGGAGGAGGTGTTTGATGCGGTGGTTGTGGCCTCCGGCCATTATTCTAAGCCAAGGTTGCCGTCCATTAAAGGAATGGATGCATGGAAGAGGAAGCAAATGCATAGTCACATCTACAGGGTTCCGGAGCCATTTCGTGATGAGGTTGTGGTGGTGGTTGGAAATTCAGTAAGTGGACAAGATATATCAATGGAACTAGTGGATGTGGCCAAGGCAATCTATCTCAGTGCCAAATCTCTTGACATATCTGAGGGCTTGTCCAAAGTCATTTCCAAACACGAAAACTTGCACCTTCGTCCCCAGATAGAGTCACTCCAAGAAGATGCGAGTGTATTATTTGTGAACGGTTCTTGGGTCATTGCAGACACTATTATATACTGTACTGGGTATTCATACACATTCCCCTTTCTTGACACCAAGGGAATAGTTGCTATAGATGATAACAGAGTGGGCCCTCTGTATGAGCACACCTTCCCTCCATCACTTGCACCCTCTTTATCCTTTATAGGCATTGCAACAAAGATCATAGCGTTCCCTTTCTTTGAGTCACAAGCAAAATGGATAGCTCAACTACTCTCTGGCAAAACAACATTGCCATCAAGGGATGACATGATGCATTCCATCAAGGAGTTGTACCACTCAAGGGAGATTGCTGGCATTCCGAAGCATAACACCCACGAGATCGGCGACTTCGAGTATTGTGATAGATATGGGGATCACATAGGGTTCCCACGTTTGGAAGAATGGAGGAAAAAGATTTGCCTTTCGACAATAAGAAATGCTGAGACCGACTTAGAAACATATAAGGATTCGTGGGACGATCATGAGCTGCTTCAAGAGGCTCTTCAAAGTCCACATTTCACTCAACTTGGGCCTCAAGATTTAGATTTTCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FMO-like K_oxygenase

Protein Analysis

462

Amino Acids

52.34

Weight (kDa)

5.65

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 5 - 47 3.8e-06 Thi4 family
FMO-like PF00743 9 - 241 1.3e-31 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 10 - 300 9.5e-16 Pyridine nucleotide-disulphide oxidoreductase
HI0933_like PF03486 10 - 44 8.6e-06 HI0933-like protein Rossmann domain
FAD_binding_3 PF01494 10 - 41 5.9e-06 FAD binding domain
DAO PF01266 11 - 45 9.1e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 13 - 47 2.7e-10 NAD(P)-binding Rossmann-like domain
Pyr_redox_3 PF13738 168 - 316 6.5e-13 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 258 - 380 3.5e-18 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011359)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07800
fragaria_vesca FvH4_5g14390
malus_domestica MD06G1199100.v1.1 MD14G1206500.v1.1 MD14G1206800.v1.1 MD14G1207200.v1.1
prunus_persica Prupe.5G199900_v2.0.a1
pyrus_communis pycom14g17100 pycom14g17110 pycom14g17120
rosa_chinensis RchiOBHm_Chr7g0182301
rosa_laevigata RLG00000005134
rosa_multiflora Rmu_sc0000003.1_g000007
rosa_roxburghii Rroxscaffold_3G00271340
rosa_rugosa Rorug06G0446900
rosa_samantha Rh7AG049800 Rh7BG049900 Rh7CG051200 Rh7DG049100
rosa_wichuraiana Rw7G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 127
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 1 cut(s) 506
AclWI GGATC 3 cut(s) 164, 177, 1209
AcoI YGGCCR 3 cut(s) 310, 523, 693
AcsI RAATTY 1 cut(s) 649
AdeI CACNNNGTG 1 cut(s) 100
AfaI GTAC 4 cut(s) 146, 332, 868, 1121
AfiI CCNNNNNNNGG 6 cut(s) 127, 157, 180, 181, 1129, 1222
AflIII ACRYGT 1 cut(s) 216
AgsI TTSAA 4 cut(s) 382, 445, 1322, 1334
AhlI ACTAGT 1 cut(s) 682
AjiI CACGTC 1 cut(s) 219
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 6 cut(s) 47, 68, 79, 322, 1052, 1315
AluI AGCT 6 cut(s) 47, 68, 79, 322, 1052, 1315
Alw21I GWGCWC 1 cut(s) 954
Alw26I GTCTC 2 cut(s) 220, 1265
AlwI GGATC 3 cut(s) 164, 177, 1209
AlwNI CAGNNNCTG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 6 cut(s) 310, 516, 523, 693, 937, 1358
ApaI GGGCCC 1 cut(s) 941
ApeKI GCWGC 2 cut(s) 68, 1315
ApoI RAATTY 1 cut(s) 649
ArsI GACNNNNNNTTYG 4 cut(s) 254, 286, 577, 609
AspA2I CCTAGG 1 cut(s) 175
AspS9I GGNCC 4 cut(s) 50, 937, 938, 1358
AvaII GGWCC 1 cut(s) 50
AvrII CCTAGG 1 cut(s) 175
AxyI CCTNAGG 1 cut(s) 55
BaeGI GKGCMC 2 cut(s) 187, 941
BalI TGGCCA 2 cut(s) 312, 695
BamHI GGATCC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 941
BarI GAAGNNNNNNTAC 2 cut(s) 172, 204
BauI CACGAG 1 cut(s) 1163
BbsI GAAGAC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 954
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 55, 1302
BccI CCATC 4 cut(s) 407, 973, 1090, 1115
BceAI ACGGC 1 cut(s) 532
BciT130I CCWGG 1 cut(s) 309
BcoDI GTCTC 2 cut(s) 220, 1265
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 2 cut(s) 176, 683
BfmI CTRYAG 2 cut(s) 601, 918
BisI GCNGC 2 cut(s) 69, 1316
BlnI CCTAGG 1 cut(s) 175
BlsI GCNGC 2 cut(s) 70, 1317
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 50
BmgBI CACGTC 1 cut(s) 219
BmgT120I GGNCC 4 cut(s) 50, 937, 938, 1358
BmiI GGNNCC 7 cut(s) 52, 171, 186, 609, 615, 939, 1214
BmrFI CCNGG 1 cut(s) 309
BmrI ACTGGG 1 cut(s) 879
BmsI GCATC 4 cut(s) 493, 556, 799, 1089
BmuI ACTGGG 1 cut(s) 879
BpiI GAAGAC 1 cut(s) 479
Bpu10I CCTNAGC 1 cut(s) 80
BpuEI CTTGAG 3 cut(s) 320, 1111, 1347
BsaBI GATNNNNATC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 1265
BsaJI CCNNGG 4 cut(s) 175, 539, 696, 903
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 4 cut(s) 482, 512, 946, 976
Bsc4I CCNNNNNNNGG 6 cut(s) 127, 157, 180, 181, 1129, 1222
Bse1I ACTGG 1 cut(s) 874
Bse21I CCTNAGG 1 cut(s) 55
Bse3DI GCAATG 3 cut(s) 843, 996, 1076
Bse8I GATNNNNATC 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 175, 539, 696, 903
BseGI GGATG 5 cut(s) 411, 418, 571, 694, 1096
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 6 cut(s) 127, 157, 180, 181, 1129, 1222
BseMI GCAATG 3 cut(s) 843, 996, 1076
BseMII CTCAG 5 cut(s) 69, 71, 724, 726, 1260
BseNI ACTGG 1 cut(s) 874
BseRI GAGGAG 3 cut(s) 313, 472, 506
BseSI GKGCMC 2 cut(s) 187, 941
BseXI GCAGC 2 cut(s) 55, 1302
BshFI GGCC 6 cut(s) 312, 518, 525, 695, 939, 1360
BshNI GGYRCC 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 954
BsiSI CCGG 2 cut(s) 522, 611
BslFI GGGAC 3 cut(s) 305, 768, 1316
BslI CCNNNNNNNGG 6 cut(s) 127, 157, 180, 181, 1129, 1222
BsmAI GTCTC 2 cut(s) 220, 1265
BsmFI GGGAC 3 cut(s) 305, 768, 1316
BsmI GAATGC 2 cut(s) 1102, 1143
BsnI GGCC 6 cut(s) 312, 518, 525, 695, 939, 1360
Bso31I GGTCTC 1 cut(s) 1265
Bsp120I GGGCCC 1 cut(s) 937
Bsp1286I GDGCHC 3 cut(s) 187, 941, 954
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 7 cut(s) 169, 246, 418, 1008, 1167, 1201, 1306
BspACI CCGC 1 cut(s) 506
BspANI GGCC 6 cut(s) 312, 518, 525, 695, 939, 1360
BspCNI CTCAG 5 cut(s) 68, 72, 723, 727, 1261
BspEI TCCGGA 1 cut(s) 610
BspHI TCATGA 1 cut(s) 1309
BspLI GGNNCC 7 cut(s) 52, 171, 186, 609, 615, 939, 1214
BspPI GGATC 3 cut(s) 164, 177, 1209
BspQI GCTCTTC 1 cut(s) 1335
BspT107I GGYRCC 1 cut(s) 184
BspTNI GGTCTC 1 cut(s) 1265
BsrDI GCAATG 3 cut(s) 843, 996, 1076
BsrI ACTGG 1 cut(s) 874
BssECI CCNNGG 4 cut(s) 175, 539, 696, 903
BssMI GATC 7 cut(s) 169, 246, 418, 1008, 1167, 1201, 1306
BssSI CACGAG 1 cut(s) 1163
BssT1I CCWWGG 4 cut(s) 175, 539, 696, 903
Bst2BI CACGAG 1 cut(s) 1163
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 833, 866
Bst6I CTCTTC 2 cut(s) 569, 1335
BstC8I GCNNGC 1 cut(s) 1141
BstDEI CTNAG 7 cut(s) 55, 80, 534, 710, 735, 1269, 1279
BstF5I GGATG 5 cut(s) 411, 418, 571, 694, 1096
BstKTI GATC 7 cut(s) 172, 249, 421, 1011, 1170, 1204, 1309
BstMAI GTCTC 2 cut(s) 220, 1265
BstMBI GATC 7 cut(s) 169, 246, 418, 1008, 1167, 1201, 1306
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 2 cut(s) 601, 918
BstSLI GKGCMC 2 cut(s) 187, 941
BstV1I GCAGC 2 cut(s) 55, 1302
BstV2I GAAGAC 1 cut(s) 479
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
Bsu36I CCTNAGG 1 cut(s) 55
BsuRI GGCC 6 cut(s) 312, 518, 525, 695, 939, 1360
BtrI CACGTC 1 cut(s) 219
BtsCI GGATG 5 cut(s) 411, 418, 571, 694, 1096
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 3 cut(s) 143, 258, 718
Cac8I GCNNGC 1 cut(s) 1141
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 1 cut(s) 1309
Cfr13I GGNCC 4 cut(s) 50, 937, 938, 1358
Csp6I GTAC 4 cut(s) 145, 331, 867, 1120
CviAII CATG 7 cut(s) 122, 232, 295, 422, 570, 1096, 1310
CviQI GTAC 4 cut(s) 145, 331, 867, 1120
DdeI CTNAG 7 cut(s) 55, 80, 534, 710, 735, 1269, 1279
DpnI GATC 7 cut(s) 171, 248, 420, 1010, 1169, 1203, 1308
DpnII GATC 7 cut(s) 169, 246, 418, 1008, 1167, 1201, 1306
DraIII CACNNNGTG 1 cut(s) 100
EaeI YGGCCR 3 cut(s) 310, 523, 693
Eam1104I CTCTTC 2 cut(s) 569, 1335
EarI CTCTTC 2 cut(s) 569, 1335
Eco130I CCWWGG 4 cut(s) 175, 539, 696, 903
Eco24I GRGCYC 1 cut(s) 941
Eco31I GGTCTC 1 cut(s) 1265
Eco47I GGWCC 1 cut(s) 50
Eco81I CCTNAGG 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 938
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 4 cut(s) 175, 539, 696, 903
EcoT22I ATGCAT 3 cut(s) 571, 591, 1104
EcoT38I GRGCYC 1 cut(s) 941
ErhI CCWWGG 4 cut(s) 175, 539, 696, 903
FaeI CATG 7 cut(s) 125, 235, 298, 425, 573, 1099, 1313
FalI AAGNNNNNCTT 2 cut(s) 1314, 1346
FaqI GGGAC 3 cut(s) 305, 768, 1316
FatI CATG 7 cut(s) 121, 231, 294, 421, 569, 1095, 1309
FauNDI CATATG 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 69, 1316
FokI GGATG 5 cut(s) 418, 425, 578, 701, 1103
FriOI GRGCYC 1 cut(s) 941
Fsp4HI GCNGC 2 cut(s) 69, 1316
FspBI CTAG 2 cut(s) 176, 683
GluI GCNGC 2 cut(s) 69, 1316
HaeIII GGCC 6 cut(s) 312, 518, 525, 695, 939, 1360
HapII CCGG 2 cut(s) 522, 611
Hin1II CATG 7 cut(s) 125, 235, 298, 425, 573, 1099, 1313
HinfI GANTC 4 cut(s) 228, 794, 1031, 1295
HpaII CCGG 2 cut(s) 522, 611
Hpy166II GTNNAC 4 cut(s) 258, 662, 829, 1340
Hpy188I TCNGA 3 cut(s) 10, 736, 1149
Hpy188III TCNNGA 8 cut(s) 280, 611, 623, 725, 896, 1310, 1322, 1364
Hpy8I GTNNAC 4 cut(s) 258, 662, 829, 1340
HpyAV CCTTC 4 cut(s) 83, 174, 788, 967
HpyCH4III ACNGT 2 cut(s) 833, 866
HpyCH4IV ACGT 2 cut(s) 218, 1219
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 7 cut(s) 55, 80, 534, 710, 735, 1269, 1279
HpySE526I ACGT 2 cut(s) 218, 1219
Hsp92II CATG 7 cut(s) 125, 235, 298, 425, 573, 1099, 1313
Kpn2I TCCGGA 1 cut(s) 610
Kzo9I GATC 7 cut(s) 169, 246, 418, 1008, 1167, 1201, 1306
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 4 cut(s) 44, 76, 319, 613
Lsp1109I GCAGC 2 cut(s) 55, 1302
LweI GCATC 4 cut(s) 493, 556, 799, 1089
MaeI CTAG 2 cut(s) 176, 683
MaeII ACGT 2 cut(s) 218, 1219
MaeIII GTNAC 4 cut(s) 92, 593, 795, 1032
MalI GATC 7 cut(s) 171, 248, 420, 1010, 1169, 1203, 1308
MboI GATC 7 cut(s) 169, 246, 418, 1008, 1167, 1201, 1306
MboII GAAGA 6 cut(s) 481, 484, 586, 818, 1238, 1322
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 3 cut(s) 187, 941, 954
MlsI TGGCCA 2 cut(s) 312, 695
MluCI AATT 2 cut(s) 13, 649
MluNI TGGCCA 2 cut(s) 312, 695
MlyI GAGTC 3 cut(s) 222, 803, 1040
MmeI TCCRAC 2 cut(s) 390, 625
Mox20I TGGCCA 2 cut(s) 312, 695
Mph1103I ATGCAT 3 cut(s) 571, 591, 1104
MroI TCCGGA 1 cut(s) 610
MscI TGGCCA 2 cut(s) 312, 695
MseI TTAA 2 cut(s) 371, 555
Msp20I TGGCCA 2 cut(s) 312, 695
MspI CCGG 2 cut(s) 522, 611
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 2 cut(s) 1102, 1143
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 1 cut(s) 544
NdeI CATATG 1 cut(s) 397
NdeII GATC 7 cut(s) 169, 246, 418, 1008, 1167, 1201, 1306
NlaIII CATG 7 cut(s) 125, 235, 298, 425, 573, 1099, 1313
NlaIV GGNNCC 7 cut(s) 52, 171, 186, 609, 615, 939, 1214
NmuCI GTSAC 4 cut(s) 92, 593, 795, 1032
NsiI ATGCAT 3 cut(s) 571, 591, 1104
PagI TCATGA 1 cut(s) 1309
PciSI GCTCTTC 1 cut(s) 1335
PcsI WCGNNNNNNNCGW 2 cut(s) 1170, 1176
PctI GAATGC 2 cut(s) 1102, 1143
PfeI GAWTC 1 cut(s) 1295
PflMI CCANNNNNTGG 1 cut(s) 127
PkrI GCNGC 2 cut(s) 70, 1317
PleI GAGTC 3 cut(s) 222, 802, 1039
PpsI GAGTC 3 cut(s) 222, 802, 1039
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 7 cut(s) 52, 171, 186, 609, 615, 939, 1214
PspOMI GGGCCC 1 cut(s) 937
PspPI GGNCC 4 cut(s) 50, 937, 938, 1358
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 4 cut(s) 146, 332, 868, 1121
RsaNI GTAC 4 cut(s) 145, 331, 867, 1120
SapI GCTCTTC 1 cut(s) 1335
SaqAI TTAA 2 cut(s) 371, 555
SatI GCNGC 2 cut(s) 69, 1316
Sau3AI GATC 7 cut(s) 169, 246, 418, 1008, 1167, 1201, 1306
Sau96I GGNCC 4 cut(s) 50, 937, 938, 1358
SchI GAGTC 3 cut(s) 222, 803, 1040
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 3 cut(s) 187, 941, 954
SfaNI GCATC 4 cut(s) 493, 556, 799, 1089
SfcI CTRYAG 2 cut(s) 601, 918
SinI GGWCC 1 cut(s) 50
SmlI CTYRAG 3 cut(s) 335, 1126, 1362
SmoI CTYRAG 3 cut(s) 335, 1126, 1362
SpeI ACTAGT 1 cut(s) 682
Sse9I AATT 2 cut(s) 13, 649
SsiI CCGC 1 cut(s) 506
SspMI CTAG 2 cut(s) 176, 683
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 4 cut(s) 175, 539, 696, 903
TaaI ACNGT 2 cut(s) 833, 866
TaiI ACGT 2 cut(s) 221, 1222
TaqI TCGA 3 cut(s) 279, 1179, 1253
TaqII GACCGA 2 cut(s) 491, 1289
TasI AATT 2 cut(s) 13, 649
TatI WGTACW 1 cut(s) 866
TfiI GAWTC 1 cut(s) 1295
Tru1I TTAA 2 cut(s) 371, 555
Tru9I TTAA 2 cut(s) 371, 555
TscAI CASTG 3 cut(s) 143, 265, 718
TseFI GTSAC 4 cut(s) 92, 593, 795, 1032
TseI GCWGC 2 cut(s) 68, 1315
Tsp45I GTSAC 4 cut(s) 92, 593, 795, 1032
TspDTI ATGAA 2 cut(s) 384, 867
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 3 cut(s) 143, 265, 718
Van91I CCANNNNNTGG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 50
XapI RAATTY 1 cut(s) 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XmaJI CCTAGG 1 cut(s) 175
XspI CTAG 2 cut(s) 176, 683
Zsp2I ATGCAT 3 cut(s) 571, 591, 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.