pycom14g17120

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011359
Physical Location & Seq
Reverse (-)
19191812 .. 19195020
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1386 bp
ATGGCTTCTGAAATTTACCAATCCAAAAATGTATGCGTGATTGGAGCTGGACCCTCAGGACTTGTAGCTGCAAGGGAGCTGAGGAACGAAGGTCACAGGGTGGTGGTGCTGGAACAAAACCATGATGTGGGAGGGCAGTGGCTGTACGACCCAAATGTGGAGGGAGAGGATCCCTTAGGAAGGGCACCTACCCTGAAAGTGCATAGCAGTCTTTACACGTCTTTGAGGCTCATGCTTCCAAGAGAGATCGCGGGGTTCACTGACTTTCCATTTGCAGTCAAGAAAGGTAGGGACATGAGGAGGTTTCCTGGCCATACGGAGCTTCTTTTCTACCTCAAGGATTTTTGTGATTGGTTTGGGTTGAGGGAGTTGATAAGGTTCAATACGAGGGTTTCATATGTGGGGATGTTGGATGGAGATGCAGTTGGTTGCAAAGATTTGAAATGGGTTGTGAGGAGTGTGGAGAAGAAGACCGAGATTTTCGTGGAGGAGGTGTTTGATGCGGTGGTTGTGGCCTCCGGCCATTACTCTAAGCCAAGGTTGCCGTCCATTAAAGGAATGGATGCATGGAAGAGGAAGCAAATGCATAGTCACATCTACAGGGTTCCGGAGCCATTTTGTGATGAGGTTGTGGTGGTGGTTGGAAATTCAATAAGCGGACAAGATATATCAATGGAACTAATGGATGTGGCCAAGGCAATCTATCTCAGTGCCAAATCTCTTGACATATCTGAGGGCTTGTCCAAAGTCATTTCCAAACACGAAAACTTGCACCTTCGTCCCCAGATAGAGTCACTTCAAGAAGATGGGAATGTATTATTTGTGAACGGTTCTTGGGTCATTGCAGACACTATCATATACTGTACTGGGTATTCATACGCATTCCCCTTTCTTGACACCAAAGGAATAGTTGCCATAGATGATAACAGAGTGGGCCCTCTGTATGAGCACACCTTCCCTCCATCACTTGCACCCTCTTTATCCTTTATAGGCATTGCAACAAAGATCATAGGGTTCCCTTTCTTTGAGTCACAAGCAAAATGGATAGCTCAACTACTCTCTGGCAAAACATCATTGCCATCAAGGGATGATATGATGCATTCCATCAAGGAGTTCTACCACTCAAAGGAGATTGCTGGCATTCCAAAGCATAACACCCACGAGATCGGCAACTTCGAGTATTGTGACAGATATGGGGATCACATAGGATTCCCACGTTTGGAAGAATGGAGGAAAAAGATTTGCCTTTCGGCAATAAGAAATGCTGAGACTGACTTAGAAACATACAAGGATTCGTGGGACGATCATGAGCTAATTCAAGAGGCTCTTCAAAGTCCACATTTCACTCAACTTGGGCCTCAAGATTTAGATTTTCCTCTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FMO-like K_oxygenase

Protein Analysis

462

Amino Acids

52.18

Weight (kDa)

5.73

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 6 - 47 9e-06 Thi4 family
FMO-like PF00743 9 - 240 7.8e-31 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 10 - 298 7.1e-15 Pyridine nucleotide-disulphide oxidoreductase
HI0933_like PF03486 10 - 44 8.6e-06 HI0933-like protein Rossmann domain
FAD_binding_3 PF01494 10 - 41 5.9e-06 FAD binding domain
DAO PF01266 11 - 45 9.1e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 13 - 47 2.8e-10 NAD(P)-binding Rossmann-like domain
Pyr_redox_3 PF13738 168 - 315 2.2e-11 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 257 - 379 5.1e-17 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011359)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07800
fragaria_vesca FvH4_5g14390
malus_domestica MD06G1199100.v1.1 MD14G1206500.v1.1 MD14G1206800.v1.1 MD14G1207200.v1.1
prunus_persica Prupe.5G199900_v2.0.a1
pyrus_communis pycom14g17100 pycom14g17110 pycom14g17120
rosa_chinensis RchiOBHm_Chr7g0182301
rosa_laevigata RLG00000005134
rosa_multiflora Rmu_sc0000003.1_g000007
rosa_roxburghii Rroxscaffold_3G00271340
rosa_rugosa Rorug06G0446900
rosa_samantha Rh7AG049800 Rh7BG049900 Rh7CG051200 Rh7DG049100
rosa_wichuraiana Rw7G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 127
AccII CGCG 1 cut(s) 251
AccIII TCCGGA 1 cut(s) 607
AciI CCGC 3 cut(s) 251, 503, 657
AclWI GGATC 3 cut(s) 164, 177, 1206
AcoI YGGCCR 3 cut(s) 310, 520, 690
AcsI RAATTY 2 cut(s) 12, 646
AdeI CACNNNGTG 1 cut(s) 100
AfaI GTAC 2 cut(s) 146, 865
AfiI CCNNNNNNNGG 5 cut(s) 127, 157, 180, 1126, 1219
AflIII ACRYGT 1 cut(s) 216
AgsI TTSAA 6 cut(s) 382, 442, 651, 800, 1319, 1331
AjiI CACGTC 1 cut(s) 219
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 6 cut(s) 47, 68, 79, 322, 1049, 1312
AluI AGCT 6 cut(s) 47, 68, 79, 322, 1049, 1312
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 1 cut(s) 1262
AlwI GGATC 3 cut(s) 164, 177, 1206
AlwNI CAGNNNCTG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 607
AoxI GGCC 6 cut(s) 310, 513, 520, 690, 934, 1355
ApaI GGGCCC 1 cut(s) 938
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 2 cut(s) 12, 646
ArsI GACNNNNNNTTYG 4 cut(s) 254, 286, 574, 606
AspS9I GGNCC 4 cut(s) 50, 934, 935, 1355
AvaII GGWCC 1 cut(s) 50
AxyI CCTNAGG 2 cut(s) 55, 175
BaeGI GKGCMC 2 cut(s) 187, 938
BalI TGGCCA 2 cut(s) 312, 692
BamHI GGATCC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 938
BarI GAAGNNNNNNTAC 2 cut(s) 172, 204
BauI CACGAG 1 cut(s) 1160
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 951
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 55
BccI CCATC 5 cut(s) 407, 800, 970, 1087, 1112
BceAI ACGGC 1 cut(s) 529
BciT130I CCWGG 1 cut(s) 309
BcoDI GTCTC 1 cut(s) 1262
BfmI CTRYAG 1 cut(s) 598
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 50
BmgBI CACGTC 1 cut(s) 219
BmgT120I GGNCC 4 cut(s) 50, 934, 935, 1355
BmiI GGNNCC 7 cut(s) 52, 171, 186, 606, 612, 936, 1016
BmrFI CCNGG 1 cut(s) 309
BmrI ACTGGG 1 cut(s) 876
BmsI GCATC 4 cut(s) 409, 490, 553, 1086
BmuI ACTGGG 1 cut(s) 876
BpiI GAAGAC 1 cut(s) 476
Bpu10I CCTNAGC 1 cut(s) 80
BpuEI CTTGAG 2 cut(s) 320, 1344
BsaJI CCNNGG 2 cut(s) 536, 693
BsaWI WCCGGW 1 cut(s) 607
BsaXI ACNNNNNCTCC 6 cut(s) 408, 438, 479, 509, 943, 973
Bsc4I CCNNNNNNNGG 5 cut(s) 127, 157, 180, 1126, 1219
Bse1I ACTGG 1 cut(s) 871
Bse21I CCTNAGG 2 cut(s) 55, 175
Bse3DI GCAATG 3 cut(s) 840, 993, 1073
BseAI TCCGGA 1 cut(s) 607
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 536, 693
BseGI GGATG 5 cut(s) 411, 418, 568, 691, 1093
BseLI CCNNNNNNNGG 5 cut(s) 127, 157, 180, 1126, 1219
BseMI GCAATG 3 cut(s) 840, 993, 1073
BseMII CTCAG 5 cut(s) 69, 71, 721, 723, 1257
BseNI ACTGG 1 cut(s) 871
BseRI GAGGAG 3 cut(s) 313, 469, 503
BseSI GKGCMC 2 cut(s) 187, 938
BseXI GCAGC 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 6 cut(s) 312, 515, 522, 692, 936, 1357
BshNI GGYRCC 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 951
BsiSI CCGG 2 cut(s) 519, 608
BslFI GGGAC 3 cut(s) 305, 765, 1313
BslI CCNNNNNNNGG 5 cut(s) 127, 157, 180, 1126, 1219
BsmAI GTCTC 1 cut(s) 1262
BsmFI GGGAC 3 cut(s) 305, 765, 1313
BsmI GAATGC 3 cut(s) 881, 1099, 1140
BsnI GGCC 6 cut(s) 312, 515, 522, 692, 936, 1357
Bsp120I GGGCCC 1 cut(s) 934
Bsp1286I GDGCHC 3 cut(s) 187, 938, 951
Bsp13I TCCGGA 1 cut(s) 607
Bsp143I GATC 6 cut(s) 169, 246, 1005, 1164, 1198, 1303
BspACI CCGC 3 cut(s) 251, 503, 657
BspANI GGCC 6 cut(s) 312, 515, 522, 692, 936, 1357
BspCNI CTCAG 5 cut(s) 68, 72, 720, 724, 1258
BspEI TCCGGA 1 cut(s) 607
BspFNI CGCG 1 cut(s) 251
BspHI TCATGA 1 cut(s) 1306
BspLI GGNNCC 7 cut(s) 52, 171, 186, 606, 612, 936, 1016
BspPI GGATC 3 cut(s) 164, 177, 1206
BspQI GCTCTTC 1 cut(s) 1332
BspT107I GGYRCC 1 cut(s) 184
BsrDI GCAATG 3 cut(s) 840, 993, 1073
BsrI ACTGG 1 cut(s) 871
BssECI CCNNGG 2 cut(s) 536, 693
BssMI GATC 6 cut(s) 169, 246, 1005, 1164, 1198, 1303
BssSI CACGAG 1 cut(s) 1160
BssT1I CCWWGG 2 cut(s) 536, 693
Bst2BI CACGAG 1 cut(s) 1160
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 830, 863
Bst6I CTCTTC 2 cut(s) 566, 1332
BstC8I GCNNGC 1 cut(s) 1138
BstDEI CTNAG 8 cut(s) 55, 80, 175, 531, 707, 732, 1266, 1276
BstENI CCTNNNNNAGG 1 cut(s) 178
BstF5I GGATG 5 cut(s) 411, 418, 568, 691, 1093
BstFNI CGCG 1 cut(s) 251
BstKTI GATC 6 cut(s) 172, 249, 1008, 1167, 1201, 1306
BstMAI GTCTC 1 cut(s) 1262
BstMBI GATC 6 cut(s) 169, 246, 1005, 1164, 1198, 1303
BstMWI GCNNNNNNNGC 1 cut(s) 541
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 1 cut(s) 598
BstSLI GKGCMC 2 cut(s) 187, 938
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 476
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
Bsu36I CCTNAGG 2 cut(s) 55, 175
BsuRI GGCC 6 cut(s) 312, 515, 522, 692, 936, 1357
BtrI CACGTC 1 cut(s) 219
BtsCI GGATG 5 cut(s) 411, 418, 568, 691, 1093
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 3 cut(s) 143, 258, 715
Cac8I GCNNGC 1 cut(s) 1138
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 1 cut(s) 1306
Cfr13I GGNCC 4 cut(s) 50, 934, 935, 1355
Csp6I GTAC 2 cut(s) 145, 864
CviAII CATG 5 cut(s) 122, 232, 295, 567, 1307
CviQI GTAC 2 cut(s) 145, 864
DdeI CTNAG 8 cut(s) 55, 80, 175, 531, 707, 732, 1266, 1276
DpnI GATC 6 cut(s) 171, 248, 1007, 1166, 1200, 1305
DpnII GATC 6 cut(s) 169, 246, 1005, 1164, 1198, 1303
DraIII CACNNNGTG 1 cut(s) 100
EaeI YGGCCR 3 cut(s) 310, 520, 690
Eam1104I CTCTTC 2 cut(s) 566, 1332
EarI CTCTTC 2 cut(s) 566, 1332
Eco130I CCWWGG 2 cut(s) 536, 693
Eco24I GRGCYC 1 cut(s) 938
Eco47I GGWCC 1 cut(s) 50
Eco81I CCTNAGG 2 cut(s) 55, 175
EcoNI CCTNNNNNAGG 1 cut(s) 178
EcoO109I RGGNCCY 1 cut(s) 935
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 536, 693
EcoT22I ATGCAT 3 cut(s) 568, 588, 1101
EcoT38I GRGCYC 1 cut(s) 938
ErhI CCWWGG 2 cut(s) 536, 693
FaeI CATG 5 cut(s) 125, 235, 298, 570, 1310
FalI AAGNNNNNCTT 2 cut(s) 1311, 1343
FaqI GGGAC 3 cut(s) 305, 765, 1313
FatI CATG 5 cut(s) 121, 231, 294, 566, 1306
FauI CCCGC 1 cut(s) 244
FauNDI CATATG 1 cut(s) 397
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 5 cut(s) 418, 425, 575, 698, 1100
FriOI GRGCYC 1 cut(s) 938
Fsp4HI GCNGC 1 cut(s) 69
GluI GCNGC 1 cut(s) 69
HaeIII GGCC 6 cut(s) 312, 515, 522, 692, 936, 1357
HapII CCGG 2 cut(s) 519, 608
Hin1II CATG 5 cut(s) 125, 235, 298, 570, 1310
HinfI GANTC 4 cut(s) 791, 1028, 1209, 1292
HpaII CCGG 2 cut(s) 519, 608
Hpy166II GTNNAC 3 cut(s) 258, 826, 1337
Hpy188I TCNGA 2 cut(s) 10, 733
Hpy188III TCNNGA 9 cut(s) 57, 280, 608, 722, 800, 893, 1307, 1319, 1361
Hpy8I GTNNAC 3 cut(s) 258, 826, 1337
HpyAV CCTTC 4 cut(s) 83, 174, 785, 964
HpyCH4III ACNGT 2 cut(s) 830, 863
HpyCH4IV ACGT 2 cut(s) 218, 1216
HpyF10VI GCNNNNNNNGC 1 cut(s) 541
HpyF3I CTNAG 8 cut(s) 55, 80, 175, 531, 707, 732, 1266, 1276
HpySE526I ACGT 2 cut(s) 218, 1216
Hsp92II CATG 5 cut(s) 125, 235, 298, 570, 1310
Kpn2I TCCGGA 1 cut(s) 607
Kzo9I GATC 6 cut(s) 169, 246, 1005, 1164, 1198, 1303
LguI GCTCTTC 1 cut(s) 1332
LmnI GCTCC 4 cut(s) 44, 76, 319, 610
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 4 cut(s) 409, 490, 553, 1086
MaeII ACGT 2 cut(s) 218, 1216
MaeIII GTNAC 5 cut(s) 92, 590, 792, 1029, 1184
MalI GATC 6 cut(s) 171, 248, 1007, 1166, 1200, 1305
MboI GATC 6 cut(s) 169, 246, 1005, 1164, 1198, 1303
MboII GAAGA 6 cut(s) 478, 481, 583, 815, 1235, 1319
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 3 cut(s) 187, 938, 951
MlsI TGGCCA 2 cut(s) 312, 692
MluCI AATT 3 cut(s) 12, 646, 1314
MluNI TGGCCA 2 cut(s) 312, 692
MlyI GAGTC 2 cut(s) 800, 1037
MmeI TCCRAC 2 cut(s) 390, 622
Mox20I TGGCCA 2 cut(s) 312, 692
Mph1103I ATGCAT 3 cut(s) 568, 588, 1101
MroI TCCGGA 1 cut(s) 607
MscI TGGCCA 2 cut(s) 312, 692
MseI TTAA 1 cut(s) 552
Msp20I TGGCCA 2 cut(s) 312, 692
MspI CCGG 2 cut(s) 519, 608
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 3 cut(s) 881, 1099, 1140
MvaI CCWGG 1 cut(s) 309
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 1 cut(s) 541
NdeI CATATG 1 cut(s) 397
NdeII GATC 6 cut(s) 169, 246, 1005, 1164, 1198, 1303
NlaIII CATG 5 cut(s) 125, 235, 298, 570, 1310
NlaIV GGNNCC 7 cut(s) 52, 171, 186, 606, 612, 936, 1016
NmuCI GTSAC 5 cut(s) 92, 590, 792, 1029, 1184
NsiI ATGCAT 3 cut(s) 568, 588, 1101
PagI TCATGA 1 cut(s) 1306
PciSI GCTCTTC 1 cut(s) 1332
PcsI WCGNNNNNNNCGW 1 cut(s) 1173
PctI GAATGC 3 cut(s) 881, 1099, 1140
PfeI GAWTC 2 cut(s) 1209, 1292
PflMI CCANNNNNTGG 1 cut(s) 127
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 2 cut(s) 799, 1036
PpsI GAGTC 2 cut(s) 799, 1036
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 7 cut(s) 52, 171, 186, 606, 612, 936, 1016
PspOMI GGGCCC 1 cut(s) 934
PspPI GGNCC 4 cut(s) 50, 934, 935, 1355
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 2 cut(s) 146, 865
RsaNI GTAC 2 cut(s) 145, 864
SapI GCTCTTC 1 cut(s) 1332
SaqAI TTAA 1 cut(s) 552
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 6 cut(s) 169, 246, 1005, 1164, 1198, 1303
Sau96I GGNCC 4 cut(s) 50, 934, 935, 1355
SchI GAGTC 2 cut(s) 800, 1037
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 3 cut(s) 187, 938, 951
SfaNI GCATC 4 cut(s) 409, 490, 553, 1086
SfcI CTRYAG 1 cut(s) 598
SinI GGWCC 1 cut(s) 50
SmlI CTYRAG 2 cut(s) 335, 1359
SmoI CTYRAG 2 cut(s) 335, 1359
Sse9I AATT 3 cut(s) 12, 646, 1314
SsiI CCGC 3 cut(s) 251, 503, 657
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 2 cut(s) 536, 693
TaaI ACNGT 2 cut(s) 830, 863
TaiI ACGT 2 cut(s) 221, 1219
TaqI TCGA 1 cut(s) 1176
TaqII GACCGA 1 cut(s) 488
TasI AATT 3 cut(s) 12, 646, 1314
TatI WGTACW 1 cut(s) 863
TfiI GAWTC 2 cut(s) 1209, 1292
Tru1I TTAA 1 cut(s) 552
Tru9I TTAA 1 cut(s) 552
TscAI CASTG 3 cut(s) 143, 265, 715
TseFI GTSAC 5 cut(s) 92, 590, 792, 1029, 1184
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 5 cut(s) 92, 590, 792, 1029, 1184
TspDTI ATGAA 2 cut(s) 384, 864
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 3 cut(s) 143, 265, 715
Van91I CCANNNNNTGG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 50
XagI CCTNNNNNAGG 1 cut(s) 178
XapI RAATTY 2 cut(s) 12, 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
Zsp2I ATGCAT 3 cut(s) 568, 588, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.