pycom14g18450

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008423
Physical Location & Seq
Reverse (-)
20100999 .. 20102564
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1566 bp
ATGGAGCTCTTCTCCCTGCAATCTTTCCTCCTCCTCCTTCTCTCCCTCTCCTTCTACATCTACCACCACTTCTTCGCTTCGTCGAATAAGTACAAACACACCGGCTTCAAAAACTACCTGATTCTCGGAACCCTACCGGAATTCCTAAAAAACCGCCACCGGTTCCTCGAATGGACAACCGAAATCCTCACCAGCTGCCCCACCAGCACCGCCATCCTCAGCCGCCCGGTAAAAATCCGCGGCATCATCACCGCCAACCCTGCCAACGTCGAGCACATGCTGAAAACCAACTTCGAGAACTACCCGAAAGGCGAAAGGTTCATATCCCTTCTCCAAGACTTTCTCGGCGGCGGAATCTTCAACTCCGACGGCGAGCTCTGGAAGGTTCAGAGGAAGACCGCCAGCTACGCCTTCAACACGAAATCGCTCCGGAACTTCGTTATGGAAAACGTTAGGGTTGAGATTGAGTCGAGGTTAATTCCGTCTCTGGACACAGCCGCCGAAACAGGACGCCGTTTGGACTTGCAAGATGTTTTGGAGCGCTTTGCTTTCGACAACGTTTGCAAGTTGGCTTTTAATGTAGACCCGAATTGTCTCGGAAGCGACGGCGGCGGAAAATCTGAGTCCACCGATTTCGATTCCAATCCCAATCCGGATCGGTTCATGCAGGCATTTGAAGAAGCGGCCACACTTATCTCCGGGCGATTTTTGTATGCGTTTCAGTTTATGCAAAAGATAAAGACGTTTTTGAACGTTGGATCAGAGCGGAGGTTAAAAGATTCAATTTCGACTGTCCATAACTTCGCGGATAAGATAATAAAGTCGAGAATTAGCGACGAACGGTCGGACGATCGTCACGATTTGTTGTCCTTGTTTATTGGTATTACCGAAAACAACAACTCGCCGGATTTTCTCCGAGACATCGTGATAAGCATCATTCTCGCCGGCCGAGACACGACCTCATCGGCTCTGACGTGGTTCTTCTGGCTCCTGTCGTCCAGACCTCACGTCGAGCGTGCCATTTTAAAGGAGCTCGAAATGATTCGGGTCCGAAACGGGAAAAGTATCGGCGATGTTTATGATTTGGACGAGCTCCGAGACATGCACTATTTGCACGCGGCGATTTCAGAGGCGATGCGGCTGTACCCTCCCGTACCGGTCGACACGAAGGCATGTCTAAACGACGATGTCTTGCCGGACGGGACAGCCGTGGGGAAGGGGTGGTTCGTGACGTACCATGCTTACGCGATGGGGCGGATGGAGAGGATATGGGGGACTAACTGCCGCGAGTATTTGCCAGAGCGGTGGCTGACCGAGGACGGCATGTGTCGGCAGGAAAGTGTTTTCCGGTATCCGATATTTCATGCCGGGCCAAGGATGTGTCTGGGGAAGGATTTGGCGTATATTCAGATGAAGTCGATTGCGGCATCGGTGATGGAGAGGTTTGTGATCGACGTACGGGATAGGGATACGTGTCCTGAGCATCTGTTGTCCTTGACTCTCAGGATGAAAGGTGGACTGCCCGTGAGTGTGAGGAGAAGGAGATTTGTAGCTGATGACCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

522

Amino Acids

59.85

Weight (kDa)

7.63

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 83 - 494 2.5e-60 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 766, 1303
AccI GTMKAC 2 cut(s) 582, 1161
AccII CGCG 5 cut(s) 240, 806, 1118, 1247, 1287
AccIII TCCGGA 2 cut(s) 429, 652
AclI AACGTT 3 cut(s) 450, 558, 753
AclWI GGATC 2 cut(s) 663, 766
AcoI YGGCCR 2 cut(s) 684, 946
AcsI RAATTY 1 cut(s) 140
AcyI GRCGYC 1 cut(s) 511
AfaI GTAC 5 cut(s) 92, 1145, 1155, 1235, 1458
AfeI AGCGCT 1 cut(s) 542
AfiI CCNNNNNNNGG 1 cut(s) 1374
AflIII ACRYGT 1 cut(s) 1472
AgeI ACCGGT 3 cut(s) 159, 1156, 1560
AgsI TTSAA 6 cut(s) 109, 361, 415, 677, 751, 783
AhdI GACNNNNNGTC 2 cut(s) 841, 1007
AjiI CACGTC 2 cut(s) 975, 1009
AluBI AGCT 7 cut(s) 7, 195, 376, 405, 1033, 1093, 1553
AluI AGCT 7 cut(s) 7, 195, 376, 405, 1033, 1093, 1553
Alw21I GWGCWC 5 cut(s) 9, 276, 378, 1035, 1095
Alw26I GTCTC 5 cut(s) 489, 599, 912, 945, 1092
AlwI GGATC 2 cut(s) 663, 766
Aor13HI TCCGGA 2 cut(s) 429, 652
Aor51HI AGCGCT 1 cut(s) 542
AoxI GGCC 3 cut(s) 684, 946, 1370
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 140
AsiGI ACCGGT 3 cut(s) 159, 1156, 1560
Asp700I GAANNNNTTC 1 cut(s) 1041
AspLEI GCGC 1 cut(s) 543
AspS9I GGNCC 2 cut(s) 1048, 1370
AsuC2I CCSGG 3 cut(s) 227, 700, 1369
AsuHPI GGTGA 3 cut(s) 181, 241, 1444
AvaII GGWCC 1 cut(s) 1048
BanII GRGCYC 4 cut(s) 9, 378, 1035, 1095
BbsI GAAGAC 1 cut(s) 401
Bbv12I GWGCWC 5 cut(s) 9, 276, 378, 1035, 1095
BbvCI CCTCAGC 1 cut(s) 218
BbvI GCAGC 1 cut(s) 182
BccI CCATC 4 cut(s) 221, 1243, 1252, 1429
BceAI ACGGC 5 cut(s) 385, 498, 622, 1193, 1336
BcgI CGANNNNNNTGC 2 cut(s) 922, 956
BciVI GTATCC 2 cut(s) 1362, 1462
BcnI CCSGG 3 cut(s) 227, 700, 1369
BcoDI GTCTC 5 cut(s) 489, 599, 912, 945, 1092
BfoI RGCGCY 1 cut(s) 544
BfuI GTATCC 2 cut(s) 1362, 1462
Bme1390I CCNGG 3 cut(s) 227, 700, 1369
Bme18I GGWCC 1 cut(s) 1048
BmeRI GACNNNNNGTC 2 cut(s) 841, 1007
BmgBI CACGTC 2 cut(s) 975, 1009
BmgT120I GGNCC 2 cut(s) 1048, 1370
BmiI GGNNCC 4 cut(s) 130, 164, 989, 1049
BmrFI CCNGG 3 cut(s) 227, 700, 1369
BmsI GCATC 5 cut(s) 252, 942, 1125, 1436, 1492
BoxI GACNNNNGTC 1 cut(s) 852
BpiI GAAGAC 1 cut(s) 401
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu10I CCTNAGC 2 cut(s) 218, 1479
BpuMI CCSGG 3 cut(s) 227, 700, 1369
BsaAI YACGTR 1 cut(s) 1473
BsaBI GATNNNNATC 2 cut(s) 642, 932
BsaHI GRCGYC 1 cut(s) 511
BsaJI CCNNGG 4 cut(s) 238, 1209, 1314, 1373
BsaWI WCCGGW 7 cut(s) 136, 159, 429, 652, 1156, 1347, 1560
Bsc4I CCNNNNNNNGG 1 cut(s) 1374
Bse118I RCCGGY 5 cut(s) 101, 159, 944, 1156, 1560
Bse8I GATNNNNATC 2 cut(s) 642, 932
BseAI TCCGGA 2 cut(s) 429, 652
BseDI CCNNGG 4 cut(s) 238, 1209, 1314, 1373
BseGI GGATG 4 cut(s) 213, 1263, 1383, 1512
BseJI GATNNNNATC 2 cut(s) 642, 932
BseLI CCNNNNNNNGG 1 cut(s) 1374
BseMII CTCAG 4 cut(s) 232, 612, 1470, 1516
BseRI GAGGAG 3 cut(s) 20, 23, 1549
BseX3I CGGCCG 1 cut(s) 946
BseXI GCAGC 1 cut(s) 182
Bsh1236I CGCG 5 cut(s) 240, 806, 1118, 1247, 1287
Bsh1285I CGRYCG 4 cut(s) 845, 853, 949, 1161
BshFI GGCC 3 cut(s) 686, 948, 1372
BshTI ACCGGT 3 cut(s) 159, 1156, 1560
BsiEI CGRYCG 4 cut(s) 845, 853, 949, 1161
BsiHKAI GWGCWC 5 cut(s) 9, 276, 378, 1035, 1095
BsiWI CGTACG 1 cut(s) 1456
BslFI GGGAC 2 cut(s) 1216, 1288
BslI CCNNNNNNNGG 1 cut(s) 1374
BsmAI GTCTC 5 cut(s) 489, 599, 912, 945, 1092
BsmBI CGTCTC 1 cut(s) 489
BsmFI GGGAC 2 cut(s) 1216, 1288
BsnI GGCC 3 cut(s) 686, 948, 1372
Bsp1286I GDGCHC 5 cut(s) 9, 276, 378, 1035, 1095
Bsp13I TCCGGA 2 cut(s) 429, 652
Bsp143I GATC 4 cut(s) 655, 758, 850, 1449
BspANI GGCC 3 cut(s) 686, 948, 1372
BspCNI CTCAG 4 cut(s) 231, 613, 1471, 1515
BspEI TCCGGA 2 cut(s) 429, 652
BspFNI CGCG 5 cut(s) 240, 806, 1118, 1247, 1287
BspLI GGNNCC 4 cut(s) 130, 164, 989, 1049
BspPI GGATC 2 cut(s) 663, 766
BspQI GCTCTTC 1 cut(s) 14
BsrBI CCGCTC 2 cut(s) 766, 1303
BsrFI RCCGGY 5 cut(s) 101, 159, 944, 1156, 1560
BssAI RCCGGY 5 cut(s) 101, 159, 944, 1156, 1560
BssECI CCNNGG 4 cut(s) 238, 1209, 1314, 1373
BssMI GATC 4 cut(s) 655, 758, 850, 1449
BssNI GRCGYC 1 cut(s) 511
BssT1I CCWWGG 1 cut(s) 1373
Bst4CI ACNGT 2 cut(s) 793, 843
Bst6I CTCTTC 1 cut(s) 14
BstACI GRCGYC 1 cut(s) 511
BstAPI GCANNNNNTGC 1 cut(s) 1111
BstBAI YACGTR 1 cut(s) 1473
BstC8I GCNNGC 6 cut(s) 374, 403, 669, 946, 1017, 1116
BstDEI CTNAG 4 cut(s) 218, 621, 1479, 1502
BstDSI CCRYGG 2 cut(s) 238, 1209
BstF5I GGATG 4 cut(s) 213, 1263, 1383, 1512
BstFNI CGCG 1 cut(s) 240
BstH2I RGCGCY 1 cut(s) 544
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 4 cut(s) 658, 761, 853, 1452
BstMAI GTCTC 5 cut(s) 489, 599, 912, 945, 1092
BstMBI GATC 4 cut(s) 655, 758, 850, 1449
BstMCI CGRYCG 4 cut(s) 845, 853, 949, 1161
BstMWI GCNNNNNNNGC 5 cut(s) 204, 260, 407, 609, 1111
BstNSI RCATGY 4 cut(s) 280, 1105, 1176, 1327
BstPAI GACNNNNGTC 1 cut(s) 852
BstSCI CCNGG 3 cut(s) 225, 698, 1367
BstUI CGCG 5 cut(s) 240, 806, 1118, 1247, 1287
BstV1I GCAGC 1 cut(s) 182
BstV2I GAAGAC 1 cut(s) 401
BstXI CCANNNNNNTGG 1 cut(s) 1305
BstZI CGGCCG 1 cut(s) 946
BsuI GTATCC 2 cut(s) 1362, 1462
BsuRI GGCC 3 cut(s) 686, 948, 1372
BtgI CCRYGG 2 cut(s) 238, 1209
BtgZI GCGATG 3 cut(s) 1086, 1148, 1262
BtrI CACGTC 2 cut(s) 975, 1009
BtsCI GGATG 4 cut(s) 213, 1263, 1383, 1512
Cac8I GCNNGC 6 cut(s) 374, 403, 669, 946, 1017, 1116
CfoI GCGC 1 cut(s) 543
Cfr10I RCCGGY 5 cut(s) 101, 159, 944, 1156, 1560
Cfr13I GGNCC 2 cut(s) 1048, 1370
Cfr42I CCGCGG 1 cut(s) 241
CseI GACGC 1 cut(s) 519
Csp6I GTAC 5 cut(s) 91, 1144, 1154, 1234, 1457
CspAI ACCGGT 3 cut(s) 159, 1156, 1560
CviAII CATG 7 cut(s) 277, 664, 1102, 1173, 1238, 1324, 1364
CviQI GTAC 5 cut(s) 91, 1144, 1154, 1234, 1457
DdeI CTNAG 4 cut(s) 218, 621, 1479, 1502
DpnI GATC 4 cut(s) 657, 760, 852, 1451
DpnII GATC 4 cut(s) 655, 758, 850, 1449
DraI TTTAAA 1 cut(s) 1026
DriI GACNNNNNGTC 2 cut(s) 841, 1007
EaeI YGGCCR 2 cut(s) 684, 946
EagI CGGCCG 1 cut(s) 946
Eam1104I CTCTTC 1 cut(s) 14
Eam1105I GACNNNNNGTC 2 cut(s) 841, 1007
EarI CTCTTC 1 cut(s) 14
EciI GGCGGA 3 cut(s) 366, 627, 1270
Ecl136II GAGCTC 4 cut(s) 7, 376, 1033, 1093
EclXI CGGCCG 1 cut(s) 946
Eco130I CCWWGG 1 cut(s) 1373
Eco24I GRGCYC 4 cut(s) 9, 378, 1035, 1095
Eco47I GGWCC 1 cut(s) 1048
Eco47III AGCGCT 1 cut(s) 542
Eco52I CGGCCG 1 cut(s) 946
Eco53kI GAGCTC 4 cut(s) 7, 376, 1033, 1093
EcoICRI GAGCTC 4 cut(s) 7, 376, 1033, 1093
EcoRI GAATTC 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 1373
EcoT38I GRGCYC 4 cut(s) 9, 378, 1035, 1095
ErhI CCWWGG 1 cut(s) 1373
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 7 cut(s) 280, 667, 1105, 1176, 1241, 1327, 1367
FaqI GGGAC 2 cut(s) 1216, 1288
FatI CATG 7 cut(s) 276, 663, 1101, 1172, 1237, 1323, 1363
FblI GTMKAC 2 cut(s) 582, 1161
FokI GGATG 4 cut(s) 200, 1270, 1390, 1519
FriOI GRGCYC 4 cut(s) 9, 378, 1035, 1095
GlaI GCGC 1 cut(s) 542
HaeII RGCGCY 1 cut(s) 544
HaeIII GGCC 3 cut(s) 686, 948, 1372
HgaI GACGC 1 cut(s) 519
HhaI GCGC 1 cut(s) 543
Hin1I GRCGYC 1 cut(s) 511
Hin1II CATG 7 cut(s) 280, 667, 1105, 1176, 1241, 1327, 1367
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HincII GTYRAC 1 cut(s) 1162
HindII GTYRAC 1 cut(s) 1162
HinfI GANTC 8 cut(s) 121, 354, 467, 623, 638, 779, 1042, 1498
HphI GGTGA 3 cut(s) 181, 241, 1444
Hpy166II GTNNAC 4 cut(s) 583, 627, 1162, 1517
Hpy8I GTNNAC 4 cut(s) 583, 627, 1162, 1517
Hpy99I CGWCG 8 cut(s) 85, 272, 371, 608, 839, 1013, 1187, 1457
HpyAV CCTTC 9 cut(s) 47, 61, 338, 376, 421, 1162, 1210, 1384, 1533
HpyCH4III ACNGT 2 cut(s) 793, 843
HpyCH4V TGCA 7 cut(s) 19, 526, 564, 667, 730, 1105, 1114
HpyF10VI GCNNNNNNNGC 5 cut(s) 204, 260, 407, 609, 1111
HpyF3I CTNAG 4 cut(s) 218, 621, 1479, 1502
Hsp92I GRCGYC 1 cut(s) 511
Hsp92II CATG 7 cut(s) 280, 667, 1105, 1176, 1241, 1327, 1367
HspAI GCGC 1 cut(s) 541
Kpn2I TCCGGA 2 cut(s) 429, 652
KroI GCCGGC 1 cut(s) 944
KroNI GCCGGC 1 cut(s) 946
KspI CCGCGG 1 cut(s) 241
Kzo9I GATC 4 cut(s) 655, 758, 850, 1449
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 6 cut(s) 4, 432, 538, 993, 1030, 1098
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 5 cut(s) 252, 942, 1125, 1436, 1492
MaeIII GTNAC 2 cut(s) 854, 1228
MalI GATC 4 cut(s) 657, 760, 852, 1451
MbiI CCGCTC 2 cut(s) 766, 1303
MboI GATC 4 cut(s) 655, 758, 850, 1449
MboII GAAGA 5 cut(s) 64, 349, 406, 689, 973
MhlI GDGCHC 5 cut(s) 9, 276, 378, 1035, 1095
MluCI AATT 5 cut(s) 140, 477, 589, 783, 828
MlyI GAGTC 3 cut(s) 476, 632, 1492
MmeI TCCRAC 3 cut(s) 390, 736, 825
MroI TCCGGA 2 cut(s) 429, 652
MroNI GCCGGC 1 cut(s) 944
MroXI GAANNNNTTC 1 cut(s) 1041
MseI TTAA 4 cut(s) 476, 576, 773, 1025
MspA1I CMGCKG 2 cut(s) 195, 240
MspR9I CCNGG 3 cut(s) 227, 700, 1369
MvnI CGCG 5 cut(s) 240, 806, 1118, 1247, 1287
MwoI GCNNNNNNNGC 5 cut(s) 204, 260, 407, 609, 1111
NaeI GCCGGC 1 cut(s) 946
NciI CCSGG 3 cut(s) 227, 700, 1369
NdeII GATC 4 cut(s) 655, 758, 850, 1449
NgoMIV GCCGGC 1 cut(s) 944
NlaIII CATG 7 cut(s) 280, 667, 1105, 1176, 1241, 1327, 1367
NlaIV GGNNCC 4 cut(s) 130, 164, 989, 1049
NmeAIII GCCGAG 3 cut(s) 324, 567, 974
NmuCI GTSAC 2 cut(s) 854, 1228
NspI RCATGY 4 cut(s) 280, 1105, 1176, 1327
PciSI GCTCTTC 1 cut(s) 14
PcsI WCGNNNNNNNCGW 3 cut(s) 855, 971, 1206
PdiI GCCGGC 1 cut(s) 946
PdmI GAANNNNTTC 1 cut(s) 1041
PfeI GAWTC 5 cut(s) 121, 354, 638, 779, 1042
Pfl23II CGTACG 1 cut(s) 1456
PflFI GACNNNGTC 1 cut(s) 1187
PinAI ACCGGT 3 cut(s) 159, 1156, 1560
Ple19I CGATCG 1 cut(s) 853
PleI GAGTC 3 cut(s) 475, 631, 1492
PpsI GAGTC 3 cut(s) 475, 631, 1492
Ppu21I YACGTR 1 cut(s) 1473
PshAI GACNNNNGTC 1 cut(s) 852
Psp124BI GAGCTC 4 cut(s) 9, 378, 1035, 1095
Psp1406I AACGTT 3 cut(s) 450, 558, 753
PspLI CGTACG 1 cut(s) 1456
PspN4I GGNNCC 4 cut(s) 130, 164, 989, 1049
PspPI GGNCC 2 cut(s) 1048, 1370
PsyI GACNNNGTC 1 cut(s) 1187
PvuI CGATCG 1 cut(s) 853
PvuII CAGCTG 1 cut(s) 195
RsaI GTAC 5 cut(s) 92, 1145, 1155, 1235, 1458
RsaNI GTAC 5 cut(s) 91, 1144, 1154, 1234, 1457
SacI GAGCTC 4 cut(s) 9, 378, 1035, 1095
SacII CCGCGG 1 cut(s) 241
SalI GTCGAC 1 cut(s) 1160
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 4 cut(s) 476, 576, 773, 1025
Sau3AI GATC 4 cut(s) 655, 758, 850, 1449
Sau96I GGNCC 2 cut(s) 1048, 1370
SchI GAGTC 3 cut(s) 476, 632, 1492
ScrFI CCNGG 3 cut(s) 227, 700, 1369
SduI GDGCHC 5 cut(s) 9, 276, 378, 1035, 1095
SfaNI GCATC 5 cut(s) 252, 942, 1125, 1436, 1492
Sfr303I CCGCGG 1 cut(s) 241
SgrBI CCGCGG 1 cut(s) 241
SinI GGWCC 1 cut(s) 1048
Sse9I AATT 5 cut(s) 140, 477, 589, 783, 828
SstI GAGCTC 4 cut(s) 9, 378, 1035, 1095
StyD4I CCNGG 3 cut(s) 225, 698, 1367
StyI CCWWGG 1 cut(s) 1373
TaaI ACNGT 2 cut(s) 793, 843
TaqII GACCGA 1 cut(s) 1328
TasI AATT 5 cut(s) 140, 477, 589, 783, 828
TatI WGTACW 1 cut(s) 90
TfiI GAWTC 5 cut(s) 121, 354, 638, 779, 1042
Tru1I TTAA 4 cut(s) 476, 576, 773, 1025
Tru9I TTAA 4 cut(s) 476, 576, 773, 1025
TseFI GTSAC 2 cut(s) 854, 1228
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 2 cut(s) 854, 1228
TspDTI ATGAA 5 cut(s) 310, 652, 1352, 1427, 1523
TspGWI ACGGA 1 cut(s) 471
Tth111I GACNNNGTC 1 cut(s) 1187
VpaK11BI GGWCC 1 cut(s) 1048
XapI RAATTY 1 cut(s) 140
XceI RCATGY 4 cut(s) 280, 1105, 1176, 1327
XmiI GTMKAC 2 cut(s) 582, 1161
XmnI GAANNNNTTC 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.