pycom14g20570

General negative regulator of transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006861
Physical Location & Seq
Forward (+)
21580746 .. 21587698
6953 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2700 bp
ATGGGAGCAAGTCGGAAGCTCCAGGGCGATCGATCGACCGCGTTCTCAAGAAGGTCCAGGAAGGCGTCGATGTCTTCGATAGCATTTGGAACAAGGTTTGTTTACGATACGGACAATGCGAACCAGAAGGAGAAGTTTGAGGCGGACCTGAAGAAGGAGATTAAGAAGCTACAGAGGTACAGGGACCAAATCAAGACTTGGATTCAGTCCAGTGAAATCAAGGATAAAAAGGTGAGTGCCTCTTATGAGCAGGCTCTGGTGGATGCTCGCAAACTCATTGAGCGCGAAATGGAAAGGTTTAAGATTTGTGAAAAGGAGACTAAAACAAAAGCATTTTCTAAAGAAGGATTGGGCCAACAACCTAAAACCGATCCGAGGGAGAAGGCCAAATCAGAGACAAGGGATTGGATAAACAACTTGGTTGGGGAGTTGGAATCGCAGATTGACAGCTTTGAAGCTGAGATTGAAGGGCTAACTTTCAAGAAAGGGAAGGGCAGGCCACCCAGACTGACCCATCTAGAGACTTCAATTACTCGGCATAAGGCACATATAATGAAGTTAGAACTTATCTTGAGGCTATTGGATAATGACGAGCTAAGTCCTGAGCAGGTCAATGATGTTAAAGACTTTTTGGAAGACTATGTGGAACGCAATCAGGAGGATTTTGATGAATTTAGTGAAGTTGATGAGCTTTACAACACCTTACCATTGGACAAGGTGGAGTCCCTTGAAGATCGATCTGGCCATTATTCCTCCTGGTCTTATCAAGGGTGCACCGATGCTTTGCTTGAAGACTTCTTTGGCACCACTCGCATCTCCAATCCCGCTGTTACTTCTACTAATCAGCTGAGTGTTTCTGTACAAGAGCCAGTTGAAGATACAGTTTCCCAGGACAGTAATGCTGATAACATTCCTAGAACTCCACCTCCTAAAAGTGGTGCGCTTTCTTCGTCTCCTGCATCAACGCCAACTGGGAGTCATGCAACTCCTGTCTCTGCAAGTGTTTCAACTCATAATCTGCCCAGTGTGCCAAGTGTTTTGGCCATTCCAGGTTCGAATGCTGTTCGGGGTGTCACAGAGAATGCAGGGGCTGCTAATTCTTCATCTCCTGTAAGCTTGTCTGCTTCTGTGAAGGAAGAAGAATTAGCAAGCTTCCCTGGCCGTAGACCATCACCATCTCTTTCTGATGCTGGACTTGTAAGGGGCATCAGTCGAGGTGGCTTCTCTGCACCAATTCCATCTAGTATCCCTGTTAGCTCTAGCAATGTGGCTCCTAGTAATAGTGCCCTTGGTGCAGTTCCTTCAGTTTCTGATGTGACCAAGAGAAATATATTGGGAGCTGATGAGAGAATTGGGAGTAGTGGTGTGGTGCAGCCGCTTGTTTCCCCCATAACTAACAGACTGATCGTGCTTCAAGCTGCCAAGGCTAGTGATGGAAGTGCTCCAGTTGATTCTAGTAATGCTAGTGAGGCTGGTATACCTGGCAGAGCATTTTCTCCGTCTATGGTCTCTGGCATGCAGTGGAGGCCTGGAAGTTCCTTCCAAAACCAAAATGAAGCAGGAATGTTTCGTGGAAGAACTGAAATAGCACCTGATCAGAGGGAGAAGTTTTTGCAGCGGCTCCAACAAGTGCAGCAACAAGGTCATAGTACCATGCTTGGCATGCCTCCTCTTGCTGGTGGAAATCATAAGCAATTTTCTGCACAGCAAAACCCACTGTTGCAACAGTTTAATTCTCAAAACTCATCAGTGTCTTCTCAAGCTGGCCCTGGACTAGGAGTACAGCCCCCAGTTCTGGGTACTGTTGCACCTACCACCTTACAACAGCAGCTAAATTCTATCCATCAACAGTCTAATCAACAGCCATTGATGTCAAGTGGACAAAAAGAAGGTGATGCAAGTCATCCAAAAGTTGAGGACCAGCAACAACAGAATATACCTGATGATTCTACTGCAGACTCTACCCCTAATTCTGGACTCGTAAAGAATCTAATGAATGAAGATGACTTGAGCGCCTCATATACAATTGATTCTCTGGCCGGAGTTTCTGGCACTTCCACAGAGCCTGCTCAAGTACCACGAGATATTGATCTCTCTCCTGGGCAACCTTTACAATCCAATCAACCTTCTGCTAGCCTTGGTGTTATTGGCAGAAGAAGTGTTTCTGATCTCGGTGCAATTGGTGATAACCTTTCTGGATCAACTGCTAATTCTGGGGGAATGCATGATCAGTTGTATAACTTGCAGATGCTTGATGCTGCTTACTACAAACTTCCACAACCCAAAGACTCAGAACGTGCAAGGAGCTATAGTCCAAGGCACCCCGCAATAACACCTCTTAGCTATCCTCAAGCACAAGCACCTATTGTTAACAATCCTGCCTTCTGGGAGCGGTTAGGTCTTGAACCATATGGCACTGATACGCTGTTCTTTGCATTTTACTATCAGCAGAACACTTATCAGCAATATTTGGCTGCAAAAGAATTGAAGAAGCAATCTTGGAGATACCACAGGAAATATAACACTTGGTTTCAACGACACGAGGAGCCAAAAGTTGCCACGGATGAATATGAACAAGGGACGTATGTGTACTTCGACTTTCATATTGCAAATGATGATCTACAACATGGATGGTGCCAAAGGATTAAAACAGAGTTCACTTTTGAATATAACTATCTTGAAGATGAACTTATTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001701 GO:0001824 GO:0001825 GO:0001829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010948 GO:0016070 GO:0016071 GO:0017148 GO:0019222 GO:0019439 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030014 GO:0030015 GO:0030154 GO:0030330 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031571 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042770 GO:0043009 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051248 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000036 GO:2000045 GO:2000112 GO:2000113 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
NOT2_3_5 Not3

Protein Analysis

900

Amino Acids

98.79

Weight (kDa)

5.48

Isoelectric Point (pI)

53.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Not3 PF04065 35 - 237 5.2e-68 Not1 N-terminal domain, CCR4-Not complex component
NOT2_3_5_C PF04153 755 - 893 7.3e-34 NOT2/NOT3/NOT5 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 598
AccB1I GGYRCC 3 cut(s) 803, 2319, 2634
AccBSI CCGCTC 1 cut(s) 2392
AccI GTMKAC 2 cut(s) 1165, 1477
AccII CGCG 2 cut(s) 41, 285
AciI CCGC 7 cut(s) 39, 143, 825, 1376, 1618, 2325, 2392
AclWI GGATC 2 cut(s) 365, 2206
AcoI YGGCCR 4 cut(s) 742, 1041, 1159, 2037
AcsI RAATTY 2 cut(s) 671, 1834
AcuI CTGAAG 2 cut(s) 170, 1287
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 7 cut(s) 179, 861, 1651, 1782, 1801, 2076, 2591
AfiI CCNNNNNNNGG 8 cut(s) 154, 375, 935, 1676, 1775, 1795, 1796, 1973
AjuI GAANNNNNNNTTGG 2 cut(s) 783, 815
AloI GAACNNNNNNTCC 2 cut(s) 910, 942
Alw21I GWGCWC 2 cut(s) 776, 1444
Alw26I GTCTC 6 cut(s) 311, 389, 515, 957, 997, 1513
Alw44I GTGCAC 1 cut(s) 772
AlwI GGATC 2 cut(s) 365, 2206
AlwNI CAGNNNCTG 4 cut(s) 256, 1091, 1310, 2066
AoxI GGCC 9 cut(s) 352, 384, 497, 742, 1041, 1159, 1526, 1765, 2037
ApaLI GTGCAC 1 cut(s) 772
ApeKI GCWGC 8 cut(s) 1091, 1372, 1418, 1615, 1633, 1828, 2258, 2474
ApoI RAATTY 2 cut(s) 671, 1834
Asp700I GAANNNNTTC 1 cut(s) 2161
AspLEI GCGC 3 cut(s) 285, 943, 2015
AspS9I GGNCC 6 cut(s) 54, 145, 184, 352, 1766, 1918
AsuHPI GGTGA 4 cut(s) 244, 1164, 1904, 2195
AsuII TTCGAA 1 cut(s) 1055
AsuNHI GCTAGC 1 cut(s) 2132
AvaII GGWCC 4 cut(s) 54, 145, 184, 1918
BaeGI GKGCMC 2 cut(s) 776, 1288
BalI TGGCCA 2 cut(s) 744, 1043
BanI GGYRCC 3 cut(s) 803, 2319, 2634
BarI GAAGNNNNNNTAC 2 cut(s) 2576, 2608
BauI CACGAG 2 cut(s) 2079, 2540
BbsI GAAGAC 4 cut(s) 66, 642, 798, 1746
Bbv12I GWGCWC 2 cut(s) 776, 1444
BbvI GCAGC 8 cut(s) 1078, 1384, 1405, 1627, 1645, 1840, 2245, 2461
BccI CCATC 7 cut(s) 522, 1177, 1183, 1246, 1427, 1851, 2625
BceAI ACGGC 1 cut(s) 1146
BciVI GTATCC 1 cut(s) 1256
BclI TGATCA 2 cut(s) 1594, 2227
BcoDI GTCTC 6 cut(s) 311, 389, 515, 957, 997, 1513
BfmI CTRYAG 3 cut(s) 170, 1953, 2308
BfoI RGCGCY 1 cut(s) 2016
BfuAI ACCTGC 1 cut(s) 598
BfuI GTATCC 1 cut(s) 1256
Bme18I GGWCC 4 cut(s) 54, 145, 184, 1918
BmgT120I GGNCC 6 cut(s) 54, 145, 184, 352, 1766, 1918
BmiI GGNNCC 7 cut(s) 185, 805, 1272, 1622, 2321, 2547, 2636
BmrI ACTGGG 3 cut(s) 981, 1017, 1784
BmsI GCATC 9 cut(s) 253, 769, 822, 968, 1177, 1215, 1885, 2238, 2245
BmtI GCTAGC 1 cut(s) 2136
BmuI ACTGGG 3 cut(s) 981, 1017, 1784
BpiI GAAGAC 4 cut(s) 66, 642, 798, 1746
BpmI CTGGAG 2 cut(s) 5, 1428
Bpu10I CCTNAGC 1 cut(s) 603
Bpu14I TTCGAA 1 cut(s) 1055
BpuEI CTTGAG 6 cut(s) 31, 592, 1743, 2029, 2055, 2334
Bsa29I ATCGAT 2 cut(s) 31, 736
BsaBI GATNNNNATC 1 cut(s) 2088
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 1513
BsaXI ACNNNNNCTCC 6 cut(s) 910, 940, 1348, 1378, 2296, 2326
Bsc4I CCNNNNNNNGG 8 cut(s) 154, 375, 935, 1676, 1775, 1795, 1796, 1973
Bse1I ACTGG 6 cut(s) 210, 869, 976, 1023, 1445, 1790
Bse3DI GCAATG 1 cut(s) 1270
Bse8I GATNNNNATC 1 cut(s) 2088
BseCI ATCGAT 2 cut(s) 31, 736
BseGI GGATG 4 cut(s) 268, 1903, 2569, 2636
BseJI GATNNNNATC 1 cut(s) 2088
BseLI CCNNNNNNNGG 8 cut(s) 154, 375, 935, 1676, 1775, 1795, 1796, 1973
BseMI GCAATG 1 cut(s) 1270
BseMII CTCAG 4 cut(s) 450, 594, 839, 2304
BseNI ACTGG 6 cut(s) 210, 869, 976, 1023, 1445, 1790
BseRI GAGGAG 2 cut(s) 1659, 2558
BseSI GKGCMC 2 cut(s) 776, 1288
BseXI GCAGC 8 cut(s) 1078, 1384, 1405, 1627, 1645, 1840, 2245, 2461
BsgI GTGCAG 5 cut(s) 1212, 1314, 1391, 1652, 1686
Bsh1236I CGCG 2 cut(s) 41, 285
Bsh1285I CGRYCG 3 cut(s) 31, 35, 39
BshFI GGCC 9 cut(s) 354, 386, 499, 744, 1043, 1161, 1528, 1767, 2039
BshNI GGYRCC 3 cut(s) 803, 2319, 2634
BshVI ATCGAT 2 cut(s) 31, 736
BsiEI CGRYCG 3 cut(s) 31, 35, 39
BsiHKAI GWGCWC 2 cut(s) 776, 1444
BsiSI CCGG 1 cut(s) 2040
BslFI GGGAC 3 cut(s) 197, 709, 2593
BslI CCNNNNNNNGG 8 cut(s) 154, 375, 935, 1676, 1775, 1795, 1796, 1973
BsmAI GTCTC 6 cut(s) 311, 389, 515, 957, 997, 1513
BsmBI CGTCTC 1 cut(s) 957
BsmFI GGGAC 3 cut(s) 197, 709, 2593
BsmI GAATGC 3 cut(s) 1063, 1087, 2226
BsnI GGCC 9 cut(s) 354, 386, 499, 744, 1043, 1161, 1528, 1767, 2039
Bso31I GGTCTC 1 cut(s) 1513
Bsp119I TTCGAA 1 cut(s) 1055
Bsp1286I GDGCHC 3 cut(s) 776, 1288, 1444
Bsp1407I TGTACA 1 cut(s) 859
BspACI CCGC 7 cut(s) 39, 143, 825, 1376, 1618, 2325, 2392
BspANI GGCC 9 cut(s) 354, 386, 499, 744, 1043, 1161, 1528, 1767, 2039
BspCNI CTCAG 4 cut(s) 451, 595, 840, 2303
BspDI ATCGAT 2 cut(s) 31, 736
BspFNI CGCG 2 cut(s) 41, 285
BspLI GGNNCC 7 cut(s) 185, 805, 1272, 1622, 2321, 2547, 2636
BspMAI CTGCAG 1 cut(s) 1957
BspMI ACCTGC 1 cut(s) 598
BspOI GCTAGC 1 cut(s) 2136
BspPI GGATC 2 cut(s) 365, 2206
BspT104I TTCGAA 1 cut(s) 1055
BspT107I GGYRCC 3 cut(s) 803, 2319, 2634
BspTNI GGTCTC 1 cut(s) 1513
BsrBI CCGCTC 1 cut(s) 2392
BsrDI GCAATG 1 cut(s) 1270
BsrGI TGTACA 1 cut(s) 859
BsrI ACTGG 6 cut(s) 210, 869, 976, 1023, 1445, 1790
BssNAI GTATAC 1 cut(s) 1478
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 2 cut(s) 2079, 2540
BssT1I CCWWGG 4 cut(s) 1288, 1422, 2137, 2315
Bst1107I GTATAC 1 cut(s) 1478
Bst2BI CACGAG 2 cut(s) 2079, 2540
Bst4CI ACNGT 6 cut(s) 883, 896, 1719, 1728, 1804, 1851
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 1091
BstAUI TGTACA 1 cut(s) 859
BstBI TTCGAA 1 cut(s) 1055
BstC8I GCNNGC 9 cut(s) 252, 268, 497, 1150, 1517, 1664, 1765, 2067, 2134
BstDEI CTNAG 6 cut(s) 459, 596, 603, 848, 2290, 2339
BstDSI CCRYGG 1 cut(s) 2559
BstENI CCTNNNNNAGG 2 cut(s) 152, 1773
BstF5I GGATG 4 cut(s) 268, 1903, 2569, 2636
BstFNI CGCG 2 cut(s) 41, 285
BstH2I RGCGCY 1 cut(s) 2016
BstHHI GCGC 3 cut(s) 285, 943, 2015
BstMAI GTCTC 6 cut(s) 311, 389, 515, 957, 997, 1513
BstMCI CGRYCG 3 cut(s) 31, 35, 39
BstMWI GCNNNNNNNGC 9 cut(s) 810, 1027, 1091, 1158, 1292, 1424, 1469, 1525, 1663
BstNSI RCATGY 2 cut(s) 1519, 1666
BstSFI CTRYAG 3 cut(s) 170, 1953, 2308
BstSLI GKGCMC 2 cut(s) 776, 1288
BstUI CGCG 2 cut(s) 41, 285
BstV1I GCAGC 8 cut(s) 1078, 1384, 1405, 1627, 1645, 1840, 2245, 2461
BstV2I GAAGAC 4 cut(s) 66, 642, 798, 1746
BstZ17I GTATAC 1 cut(s) 1478
Bsu15I ATCGAT 2 cut(s) 31, 736
BsuI GTATCC 1 cut(s) 1256
BsuRI GGCC 9 cut(s) 354, 386, 499, 744, 1043, 1161, 1528, 1767, 2039
BsuTUI ATCGAT 2 cut(s) 31, 736
BtgI CCRYGG 1 cut(s) 2559
BtsCI GGATG 4 cut(s) 268, 1903, 2569, 2636
BtsI GCAGTG 1 cut(s) 1526
BtsIMutI CAGTG 6 cut(s) 217, 1030, 1526, 1715, 1755, 2415
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 9 cut(s) 252, 268, 497, 1150, 1517, 1664, 1765, 2067, 2134
CaiI CAGNNNCTG 4 cut(s) 256, 1091, 1310, 2066
CfoI GCGC 3 cut(s) 285, 943, 2015
Cfr13I GGNCC 6 cut(s) 54, 145, 184, 352, 1766, 1918
ClaI ATCGAT 2 cut(s) 31, 736
CseI GACGC 1 cut(s) 54
Csp6I GTAC 7 cut(s) 178, 860, 1650, 1781, 1800, 2075, 2590
CviAII CATG 6 cut(s) 980, 1516, 1654, 1663, 2225, 2627
CviQI GTAC 7 cut(s) 178, 860, 1650, 1781, 1800, 2075, 2590
DdeI CTNAG 6 cut(s) 459, 596, 603, 848, 2290, 2339
EaeI YGGCCR 4 cut(s) 742, 1041, 1159, 2037
EciI GGCGGA 1 cut(s) 158
Eco130I CCWWGG 4 cut(s) 1288, 1422, 2137, 2315
Eco147I AGGCCT 1 cut(s) 1528
Eco31I GGTCTC 1 cut(s) 1513
Eco47I GGWCC 4 cut(s) 54, 145, 184, 1918
Eco57I CTGAAG 2 cut(s) 170, 1287
EcoNI CCTNNNNNAGG 2 cut(s) 152, 1773
EcoT14I CCWWGG 4 cut(s) 1288, 1422, 2137, 2315
EcoT22I ATGCAT 1 cut(s) 2226
ErhI CCWWGG 4 cut(s) 1288, 1422, 2137, 2315
Esp3I CGTCTC 1 cut(s) 957
FaeI CATG 6 cut(s) 983, 1519, 1657, 1666, 2228, 2630
FalI AAGNNNNNCTT 1 cut(s) 2673
FaqI GGGAC 3 cut(s) 197, 709, 2593
FatI CATG 6 cut(s) 979, 1515, 1653, 1662, 2224, 2626
FauI CCCGC 2 cut(s) 832, 2332
FauNDI CATATG 1 cut(s) 2410
FbaI TGATCA 2 cut(s) 1594, 2227
FblI GTMKAC 2 cut(s) 1165, 1477
FokI GGATG 4 cut(s) 275, 1890, 2576, 2643
GlaI GCGC 3 cut(s) 284, 942, 2014
GsuI CTGGAG 2 cut(s) 5, 1428
HaeII RGCGCY 1 cut(s) 2016
HaeIII GGCC 9 cut(s) 354, 386, 499, 744, 1043, 1161, 1528, 1767, 2039
HapII CCGG 1 cut(s) 2040
HgaI GACGC 1 cut(s) 54
HhaI GCGC 3 cut(s) 285, 943, 2015
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 6 cut(s) 983, 1519, 1657, 1666, 2228, 2630
Hin6I GCGC 3 cut(s) 283, 941, 2013
HinP1I GCGC 3 cut(s) 283, 941, 2013
HincII GTYRAC 1 cut(s) 2371
HindII GTYRAC 1 cut(s) 2371
HindIII AAGCTT 2 cut(s) 1114, 1150
HpaI GTTAAC 1 cut(s) 2371
HpaII CCGG 1 cut(s) 2040
HphI GGTGA 4 cut(s) 244, 1164, 1904, 2195
Hpy166II GTNNAC 8 cut(s) 103, 774, 1166, 1478, 1880, 2371, 2590, 2658
Hpy188I TCNGA 8 cut(s) 15, 375, 394, 1186, 1312, 1599, 2167, 2293
Hpy8I GTNNAC 8 cut(s) 103, 774, 1166, 1478, 1880, 2371, 2590, 2658
Hpy99I CGWCG 1 cut(s) 70
HpyCH4III ACNGT 6 cut(s) 883, 896, 1719, 1728, 1804, 1851
HpyCH4IV ACGT 2 cut(s) 2296, 2582
HpyF10VI GCNNNNNNNGC 9 cut(s) 810, 1027, 1091, 1158, 1292, 1424, 1469, 1525, 1663
HpyF3I CTNAG 6 cut(s) 459, 596, 603, 848, 2290, 2339
HpySE526I ACGT 2 cut(s) 2296, 2582
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 6 cut(s) 983, 1519, 1657, 1666, 2228, 2630
HspAI GCGC 3 cut(s) 283, 941, 2013
Ksp22I TGATCA 2 cut(s) 1594, 2227
KspAI GTTAAC 1 cut(s) 2371
LmnI GCTCC 9 cut(s) 5, 24, 1276, 1337, 1447, 1626, 2304, 2389, 2545
Lsp1109I GCAGC 8 cut(s) 1078, 1384, 1405, 1627, 1645, 1840, 2245, 2461
LweI GCATC 9 cut(s) 253, 769, 822, 968, 1177, 1215, 1885, 2238, 2245
MaeII ACGT 2 cut(s) 2296, 2582
MaeIII GTNAC 3 cut(s) 829, 1072, 1315
MbiI CCGCTC 1 cut(s) 2392
MfeI CAATTG 2 cut(s) 2025, 2178
MhlI GDGCHC 3 cut(s) 776, 1288, 1444
MlsI TGGCCA 2 cut(s) 744, 1043
MluNI TGGCCA 2 cut(s) 744, 1043
MlyI GAGTC 5 cut(s) 731, 985, 1952, 1971, 2282
MmeI TCCRAC 2 cut(s) 411, 1648
Mox20I TGGCCA 2 cut(s) 744, 1043
Mph1103I ATGCAT 1 cut(s) 2226
MroXI GAANNNNTTC 1 cut(s) 2161
MscI TGGCCA 2 cut(s) 744, 1043
MseI TTAA 6 cut(s) 162, 300, 621, 1731, 2370, 2646
Msp20I TGGCCA 2 cut(s) 744, 1043
MspA1I CMGCKG 3 cut(s) 827, 847, 1618
MspI CCGG 1 cut(s) 2040
MunI CAATTG 2 cut(s) 2025, 2178
Mva1269I GAATGC 3 cut(s) 1063, 1087, 2226
MvnI CGCG 2 cut(s) 41, 285
MwoI GCNNNNNNNGC 9 cut(s) 810, 1027, 1091, 1158, 1292, 1424, 1469, 1525, 1663
NdeI CATATG 1 cut(s) 2410
NheI GCTAGC 1 cut(s) 2132
NlaIII CATG 6 cut(s) 983, 1519, 1657, 1666, 2228, 2630
NlaIV GGNNCC 7 cut(s) 185, 805, 1272, 1622, 2321, 2547, 2636
NmeAIII GCCGAG 1 cut(s) 514
NmuCI GTSAC 2 cut(s) 1072, 1315
NsiI ATGCAT 1 cut(s) 2226
NspI RCATGY 2 cut(s) 1519, 1666
NspV TTCGAA 1 cut(s) 1055
PaeI GCATGC 2 cut(s) 1519, 1666
PceI AGGCCT 1 cut(s) 1528
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 116
PctI GAATGC 3 cut(s) 1063, 1087, 2226
PdmI GAANNNNTTC 1 cut(s) 2161
PfeI GAWTC 6 cut(s) 202, 434, 1451, 1946, 1987, 2030
PfoI TCCNGGA 1 cut(s) 56
Ple19I CGATCG 2 cut(s) 31, 35
PleI GAGTC 5 cut(s) 730, 984, 1952, 1971, 2282
PpsI GAGTC 5 cut(s) 730, 984, 1952, 1971, 2282
PspN4I GGNNCC 7 cut(s) 185, 805, 1272, 1622, 2321, 2547, 2636
PspPI GGNCC 6 cut(s) 54, 145, 184, 352, 1766, 1918
PstI CTGCAG 1 cut(s) 1957
PstNI CAGNNNCTG 4 cut(s) 256, 1091, 1310, 2066
PvuI CGATCG 2 cut(s) 31, 35
PvuII CAGCTG 1 cut(s) 847
RsaI GTAC 7 cut(s) 179, 861, 1651, 1782, 1801, 2076, 2591
RsaNI GTAC 7 cut(s) 178, 860, 1650, 1781, 1800, 2075, 2590
SaqAI TTAA 6 cut(s) 162, 300, 621, 1731, 2370, 2646
Sau96I GGNCC 6 cut(s) 54, 145, 184, 352, 1766, 1918
SchI GAGTC 5 cut(s) 731, 985, 1952, 1971, 2282
SduI GDGCHC 3 cut(s) 776, 1288, 1444
SfaNI GCATC 9 cut(s) 253, 769, 822, 968, 1177, 1215, 1885, 2238, 2245
SfcI CTRYAG 3 cut(s) 170, 1953, 2308
SfuI TTCGAA 1 cut(s) 1055
SinI GGWCC 4 cut(s) 54, 145, 184, 1918
SmlI CTYRAG 6 cut(s) 46, 571, 1758, 2008, 2070, 2349
SmoI CTYRAG 6 cut(s) 46, 571, 1758, 2008, 2070, 2349
SphI GCATGC 2 cut(s) 1519, 1666
SseBI AGGCCT 1 cut(s) 1528
SsiI CCGC 7 cut(s) 39, 143, 825, 1376, 1618, 2325, 2392
SspI AATATT 1 cut(s) 2468
StuI AGGCCT 1 cut(s) 1528
StyI CCWWGG 4 cut(s) 1288, 1422, 2137, 2315
TaaI ACNGT 6 cut(s) 883, 896, 1719, 1728, 1804, 1851
TaiI ACGT 2 cut(s) 2299, 2585
TaqI TCGA 8 cut(s) 31, 35, 68, 77, 736, 1055, 1213, 2595
TatI WGTACW 3 cut(s) 859, 1780, 2589
TauI GCSGC 2 cut(s) 1378, 1621
TfiI GAWTC 6 cut(s) 202, 434, 1451, 1946, 1987, 2030
Tru1I TTAA 6 cut(s) 162, 300, 621, 1731, 2370, 2646
Tru9I TTAA 6 cut(s) 162, 300, 621, 1731, 2370, 2646
TscAI CASTG 6 cut(s) 217, 1030, 1526, 1722, 1755, 2422
TseFI GTSAC 2 cut(s) 1072, 1315
TseI GCWGC 8 cut(s) 1091, 1372, 1418, 1615, 1633, 1828, 2258, 2474
Tsp45I GTSAC 2 cut(s) 1072, 1315
TspGWI ACGGA 3 cut(s) 125, 1488, 2576
TspRI CASTG 6 cut(s) 217, 1030, 1526, 1722, 1755, 2422
VneI GTGCAC 1 cut(s) 772
VpaK11BI GGWCC 4 cut(s) 54, 145, 184, 1918
XagI CCTNNNNNAGG 2 cut(s) 152, 1773
XapI RAATTY 2 cut(s) 671, 1834
XbaI TCTAGA 1 cut(s) 517
XceI RCATGY 2 cut(s) 1519, 1666
XmiI GTMKAC 2 cut(s) 1165, 1477
XmnI GAANNNNTTC 1 cut(s) 2161
Zsp2I ATGCAT 1 cut(s) 2226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.