pycom14g20720

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004447
Physical Location & Seq
Forward (+)
21731856 .. 21736361
4506 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGGTGGTTCAACATTGGCCGATCTATCAGCAGGCTCTCGAGGATTATTTGCCTGTCCTGTTGGGATTAATGAAAGATGGAAGCCAGCTACACCACAAGGTTCAATTTTTGTGGGTAAATCATGAGGATGATGCAGAGGAAACAGCTATGTCTAATGCATGGTACGAGGTGCTGTCAATTTTGCACTTAATGGCAATGCTATCATTATCACAAGCTAACTTGTTACTTCTTCCAAGAACATCTGCTGATGGTTATCAGCCAAAAGTAACAGAAGAGAGTAGACGAGCTTCCATTGATATTTTCCTGAAGGCAGCAGGATACCTTGATTGTGCCGTCCGACATGTTCTTCCACAGTTGCCTACTGAGCTCAAGAGAAACCTGCCAATTGACTTAGCAGAAGGAGTTCTTCGAGCGCTTTGCCTACAAGCATTAGGACAGGGTGTTGATATTCAACTTGGAATGGCAATAGATAGCACAAAAGCTACACTTGCAGTGAAGCGAAGGCTTGCATGTGAAATGGTGAAGTATTGGCAACAGGCTCAAGATAACATTATGAACCTTCCATTATTAAATGGTTGGGGTGAGAAGCATCGGCTCTTTGTGAAGTGGAAATATGTTGAGGCAAAGGCTGCTGCATATTACTATCATGGTTTGATCCTTGATGAGGGTAACACAGAAAAATTTCATGGTATGGCTGTAGCTGCTTTGCAAGCAGCAGATGAGTATTTCAAAGAAAGTAAGAAGACATGCGAAGCATTTAATGCTGCTCCCCCTTTGTCGAGAAATCCACCGCTCTGGGGAACCATGAAGTATCTATCTGAGAAAATTCCAAAAGATGCTTCAAGCAAAGTGCGGATAAACCGTGATCTGTACTCACACGAGAAAATCATGGAGACAGCACCAACATTGCCCGATTTTGCATTGGCCTTGAAACCGGATGAATTTCAACTTCCTCCAGTAGACCCCTCTTGGAACATGGAACACATGAATAAGGGCTCCAACCAGCTCAGGAATGACAGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

342

Amino Acids

38.73

Weight (kDa)

6.27

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 31 - 249 4.7e-13 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 387
AccBSI CCGCTC 1 cut(s) 793
AccI GTMKAC 2 cut(s) 280, 960
AciI CCGC 2 cut(s) 791, 853
AclWI GGATC 1 cut(s) 649
AcoI YGGCCR 1 cut(s) 17
AcsI RAATTY 3 cut(s) 680, 825, 941
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 2 cut(s) 164, 872
AfeI AGCGCT 1 cut(s) 414
AfiI CCNNNNNNNGG 2 cut(s) 664, 797
AflIII ACRYGT 1 cut(s) 340
AgsI TTSAA 7 cut(s) 11, 104, 452, 730, 843, 931, 947
AjuI GAANNNNNNNTTGG 2 cut(s) 823, 855
AluBI AGCT 8 cut(s) 88, 146, 215, 287, 367, 482, 701, 1006
AluI AGCT 8 cut(s) 88, 146, 215, 287, 367, 482, 701, 1006
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 1 cut(s) 887
AlwI GGATC 1 cut(s) 649
Ama87I CYCGRG 1 cut(s) 38
Aor51HI AGCGCT 1 cut(s) 414
AoxI GGCC 2 cut(s) 17, 924
ApeKI GCWGC 6 cut(s) 311, 629, 632, 701, 713, 764
ApoI RAATTY 3 cut(s) 680, 825, 941
AseI ATTAAT 1 cut(s) 68
Asp700I GAANNNNTTC 2 cut(s) 402, 681
AspLEI GCGC 1 cut(s) 415
AsuHPI GGTGA 2 cut(s) 532, 593
AvaI CYCGRG 1 cut(s) 38
BanII GRGCYC 2 cut(s) 369, 998
BarI GAAGNNNNNNTAC 2 cut(s) 271, 303
BauI CACGAG 1 cut(s) 878
BbsI GAAGAC 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 6 cut(s) 323, 616, 619, 688, 725, 751
BccI CCATC 2 cut(s) 71, 242
BceAI ACGGC 1 cut(s) 317
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BciVI GTATCC 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 887
BfmI CTRYAG 1 cut(s) 696
BfoI RGCGCY 1 cut(s) 416
BfuAI ACCTGC 1 cut(s) 387
BfuI GTATCC 1 cut(s) 311
BisI GCNGC 6 cut(s) 312, 630, 633, 702, 714, 765
BlsI GCNGC 6 cut(s) 313, 631, 634, 703, 715, 766
BmeT110I CYCGRG 1 cut(s) 38
BmiI GGNNCC 2 cut(s) 802, 997
BmsI GCATC 3 cut(s) 121, 598, 826
BpiI GAAGAC 1 cut(s) 749
BpmI CTGGAG 1 cut(s) 939
Bpu10I CCTNAGC 1 cut(s) 1007
BpuEI CTTGAG 2 cut(s) 353, 525
BsaBI GATNNNNATC 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 934
Bsc4I CCNNNNNNNGG 2 cut(s) 664, 797
Bse1I ACTGG 1 cut(s) 956
Bse3DI GCAATG 2 cut(s) 201, 905
Bse8I GATNNNNATC 1 cut(s) 252
BseGI GGATG 2 cut(s) 133, 943
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 664, 797
BseMI GCAATG 2 cut(s) 201, 905
BseMII CTCAG 3 cut(s) 354, 810, 1021
BseNI ACTGG 1 cut(s) 956
BseXI GCAGC 6 cut(s) 323, 616, 619, 688, 725, 751
BshFI GGCC 2 cut(s) 19, 926
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 935
BslI CCNNNNNNNGG 2 cut(s) 664, 797
BsmAI GTCTC 1 cut(s) 887
BsnI GGCC 2 cut(s) 19, 926
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 369, 998
Bsp143I GATC 3 cut(s) 21, 654, 865
BspACI CCGC 2 cut(s) 791, 853
BspANI GGCC 2 cut(s) 19, 926
BspCNI CTCAG 3 cut(s) 355, 811, 1020
BspHI TCATGA 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 802, 997
BspMI ACCTGC 1 cut(s) 387
BspPI GGATC 1 cut(s) 649
BsrBI CCGCTC 1 cut(s) 793
BsrDI GCAATG 2 cut(s) 201, 905
BsrI ACTGG 1 cut(s) 956
BssMI GATC 3 cut(s) 21, 654, 865
BssSI CACGAG 1 cut(s) 878
Bst2BI CACGAG 1 cut(s) 878
Bst4CI ACNGT 2 cut(s) 354, 863
Bst6I CTCTTC 1 cut(s) 267
BstAPI GCANNNNNTGC 2 cut(s) 629, 761
BstC8I GCNNGC 4 cut(s) 33, 86, 507, 711
BstDEI CTNAG 4 cut(s) 363, 391, 819, 1007
BstENI CCTNNNNNAGG 1 cut(s) 662
BstF5I GGATG 2 cut(s) 133, 943
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 3 cut(s) 24, 657, 868
BstMAI GTCTC 1 cut(s) 887
BstMBI GATC 3 cut(s) 21, 654, 865
BstMWI GCNNNNNNNGC 6 cut(s) 364, 488, 629, 701, 710, 761
BstNSI RCATGY 3 cut(s) 344, 513, 750
BstSFI CTRYAG 1 cut(s) 696
BstV1I GCAGC 6 cut(s) 323, 616, 619, 688, 725, 751
BstV2I GAAGAC 1 cut(s) 749
BstXI CCANNNNNNTGG 1 cut(s) 795
BsuI GTATCC 1 cut(s) 311
BsuRI GGCC 2 cut(s) 19, 926
BtsCI GGATG 2 cut(s) 133, 943
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 498
BveI ACCTGC 1 cut(s) 387
Cac8I GCNNGC 4 cut(s) 33, 86, 507, 711
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 415
Csp6I GTAC 2 cut(s) 163, 871
CspCI CAANNNNNGTGG 2 cut(s) 92, 127
CviQI GTAC 2 cut(s) 163, 871
DdeI CTNAG 4 cut(s) 363, 391, 819, 1007
DpnI GATC 3 cut(s) 23, 656, 867
DpnII GATC 3 cut(s) 21, 654, 865
EaeI YGGCCR 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 367
Eco24I GRGCYC 2 cut(s) 369, 998
Eco47III AGCGCT 1 cut(s) 414
Eco53kI GAGCTC 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 662
EcoT22I ATGCAT 1 cut(s) 160
EcoT38I GRGCYC 2 cut(s) 369, 998
FalI AAGNNNNNCTT 4 cut(s) 390, 422, 471, 503
FblI GTMKAC 2 cut(s) 280, 960
Fnu4HI GCNGC 6 cut(s) 312, 630, 633, 702, 714, 765
FokI GGATG 2 cut(s) 140, 950
FriOI GRGCYC 2 cut(s) 369, 998
Fsp4HI GCNGC 6 cut(s) 312, 630, 633, 702, 714, 765
GlaI GCGC 1 cut(s) 414
GluI GCNGC 6 cut(s) 312, 630, 633, 702, 714, 765
GsuI CTGGAG 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 2 cut(s) 19, 926
HapII CCGG 1 cut(s) 935
HhaI GCGC 1 cut(s) 415
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HpaII CCGG 1 cut(s) 935
HphI GGTGA 2 cut(s) 532, 593
Hpy166II GTNNAC 2 cut(s) 281, 961
Hpy188I TCNGA 2 cut(s) 338, 820
Hpy188III TCNNGA 7 cut(s) 38, 122, 304, 370, 542, 780, 1009
Hpy8I GTNNAC 2 cut(s) 281, 961
HpyAV CCTTC 4 cut(s) 301, 392, 495, 569
HpyCH4III ACNGT 2 cut(s) 354, 863
HpyCH4V TGCA 8 cut(s) 134, 158, 184, 491, 509, 635, 709, 920
HpyF10VI GCNNNNNNNGC 6 cut(s) 364, 488, 629, 701, 710, 761
HpyF3I CTNAG 4 cut(s) 363, 391, 819, 1007
HspAI GCGC 1 cut(s) 413
Kzo9I GATC 3 cut(s) 21, 654, 865
LmnI GCTCC 2 cut(s) 772, 1001
Lsp1109I GCAGC 6 cut(s) 323, 616, 619, 688, 725, 751
LweI GCATC 3 cut(s) 121, 598, 826
MaeIII GTNAC 3 cut(s) 222, 265, 668
MalI GATC 3 cut(s) 23, 656, 867
MbiI CCGCTC 1 cut(s) 793
MboI GATC 3 cut(s) 21, 654, 865
MboII GAAGA 5 cut(s) 221, 284, 338, 398, 754
MfeI CAATTG 1 cut(s) 384
MhlI GDGCHC 2 cut(s) 369, 998
MluCI AATT 6 cut(s) 104, 177, 384, 680, 825, 941
MmeI TCCRAC 2 cut(s) 361, 1023
MnlI CCTC 8 cut(s) 34, 118, 130, 160, 613, 658, 963, 976
Mph1103I ATGCAT 1 cut(s) 160
MroXI GAANNNNTTC 2 cut(s) 402, 681
MseI TTAA 4 cut(s) 68, 188, 569, 759
MslI CAYNNNNRTG 1 cut(s) 126
MspI CCGG 1 cut(s) 935
MunI CAATTG 1 cut(s) 384
MwoI GCNNNNNNNGC 6 cut(s) 364, 488, 629, 701, 710, 761
NdeII GATC 3 cut(s) 21, 654, 865
NlaIV GGNNCC 2 cut(s) 802, 997
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 3 cut(s) 344, 513, 750
PaeR7I CTCGAG 1 cut(s) 38
PagI TCATGA 1 cut(s) 121
PciI ACATGT 1 cut(s) 340
PdmI GAANNNNTTC 2 cut(s) 402, 681
PkrI GCNGC 6 cut(s) 313, 631, 634, 703, 715, 766
PscI ACATGT 1 cut(s) 340
PshBI ATTAAT 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 802, 997
RsaI GTAC 2 cut(s) 164, 872
RsaNI GTAC 2 cut(s) 163, 871
RseI CAYNNNNRTG 1 cut(s) 126
SacI GAGCTC 1 cut(s) 369
SaqAI TTAA 4 cut(s) 68, 188, 569, 759
SatI GCNGC 6 cut(s) 312, 630, 633, 702, 714, 765
Sau3AI GATC 3 cut(s) 21, 654, 865
SduI GDGCHC 2 cut(s) 369, 998
SfaNI GCATC 3 cut(s) 121, 598, 826
SfcI CTRYAG 1 cut(s) 696
Sfr274I CTCGAG 1 cut(s) 38
SlaI CTCGAG 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 126
SmlI CTYRAG 3 cut(s) 38, 368, 540
SmoI CTYRAG 3 cut(s) 38, 368, 540
Sse9I AATT 6 cut(s) 104, 177, 384, 680, 825, 941
SsiI CCGC 2 cut(s) 791, 853
SstI GAGCTC 1 cut(s) 369
TaaI ACNGT 2 cut(s) 354, 863
TaqI TCGA 3 cut(s) 39, 409, 779
TasI AATT 6 cut(s) 104, 177, 384, 680, 825, 941
TatI WGTACW 1 cut(s) 870
Tru1I TTAA 4 cut(s) 68, 188, 569, 759
Tru9I TTAA 4 cut(s) 68, 188, 569, 759
TscAI CASTG 1 cut(s) 498
TseI GCWGC 6 cut(s) 311, 629, 632, 701, 713, 764
TspDTI ATGAA 6 cut(s) 86, 569, 674, 821, 954, 1001
TspRI CASTG 1 cut(s) 498
VspI ATTAAT 1 cut(s) 68
XagI CCTNNNNNAGG 1 cut(s) 662
XapI RAATTY 3 cut(s) 680, 825, 941
XceI RCATGY 3 cut(s) 344, 513, 750
XhoI CTCGAG 1 cut(s) 38
XmiI GTMKAC 2 cut(s) 280, 960
XmnI GAANNNNTTC 2 cut(s) 402, 681
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.