pycom15g00230

ATP-dependent zinc metalloprotease FTSH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011868
Physical Location & Seq
Reverse (-)
129380 .. 132123
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2166 bp
ATGGCTTCCGCCACCAACGCCTTGCTCTCTTCCAATTTCTTCGGAACCACCACAATCCTCCACTCTCCTCCCACCCCTAAAACCACCTCTAAATCCAAATCATCCCTCCCACTTCCCCTGCCCTTCTTCTCCCAGCGAAGGCTTTTCGCCACCAAAAACATCTTCAACTCTAAACCCGATTCACAGCCATTCAAATCAGCAACTTCCCAAGCCACTTTAGCCGCCTTGCTCTTCTCTTCTCTCACGCCACAACCCGCCCTAGCCATAGACAACACGCCCACTCCCACTCCCACTCCGCCACTTGTGCTCCAAGCCCAGCCTTCCAAACCTAACGCCTCCTCTCCTTTCTCCCAAAACCTCCTCCTCAACGCCCCTAAGCCCCAATCCCAGGTCACCACTGACCTCCCCGAAGGCAGCCAATGGCGCTACAGCGAGTTCCTCAACGCCGTCAAGAAGGGCAAGGTCGAGAGAGTCAGGTTCGTCAAAGACGGTTCTGCCCTTCAGCTAACTGCCGTCGATGGCCGCCGCGCCTCCGTCGTTGTCCCCAACGACCCTGACCTCATCGACATTTTGGCCATGAACGGCGTCGATATTTCCGTCTCCGAAGGCGATTCGGGAGGTGGCCTATTCAATTTCATCGGCAATTTGTTGTTCCCAATCTTGGCCTTTGCCGGATTGTTCCTTCTTTTTCGGCGAGCTGGAGGAGGCCCAGGAGGGCCAGGCGGGCTGGGCGGCCCCATGGATTTCGGAAGGTCCAAGTCCAAATTCCAAGAAGTTCCAGAGACGGGGGTGACTTTCTCCGATGTAGCTGGAGCTGACCAAGCAAAATTGGAGCTGCAGGAGGTGGTGGATTTCTTGAAGAACCCAGACAAGTACACCGCCCTCGGTGCCAAAATCCCAAAAGGATGTCTTTTGGTGGGTCCACCTGGTACCGGCAAGACCCTTCTGGCTCGCGCCGTGGCCGGAGAGGCTGGGACCCCGTTTTTCTCGTGCGCGGCTTCCGAGTTCGTGGAGTTGTTTGTGGGAGTGGGGGCGTCGAGAGTGCGGGACTTGTTCGAGAAGGCCAAGTCGAAAGCGCCGTGCATTGTGTTCATCGATGAGATAGATGCGGTGGGGAGGCAGAGAGGCGCGGGGATGGGCGGCGGGAATGATGAGAGGGAGCAGACTATTAATCAGCTGCTGACGGAGATGGATGGGTTTTCTGGTAATTCCGGTGTGATAGTTTTGGCGGCAACCAACAGGCCGGATGTTCTCGACTCGGCGTTGTTGAGGCCCGGAAGGTTTGACCGACAGGTGACCGTCGACAGGCCTGATGTTGCTGGCAGAGTTAAAATACTGCAGGTGCACTCTAGAGGGAAGGCGCTTGCTAAGGATGTGGACTTTGACAAAATTGCGAGGAGAACACCCGGTTTCACTGGCGCCGATCTGCAGAACCTGATGAACGAGGCTGCCATTCTCGCAGCCAGACGTGACCTCAAAGAAATAAGTAAGGATGAGATCGCCGATGCTCTGGAGCGGATCATTGCTGGACCAGAGAAGAAAAATGCTGTTGTCTCTGAAGACAAGAAGAAATTAGTCGCCTACCATGAGGCGGGGCATGCTCTTGTTGGTGCATTGATGCCTGAATATGATCCTGTTGCTAAGATATCCATCATCCCTCGTGGCCAAGCTGGCGGTCTCACCTTCTTCGCTCCAAGTGAAGAGAGACTTGAGTCTGGACTGTACAGTAGAAGCTACCTGGAGAATCAAATGGCTGTTGCCCTCGGTGGAAGGGTTGCGGAAGAAGTTATCTTTGGTCAGGAGAATGTGACAACAGGGGCATCAAGCGACTTCATGCAAGTTTCAAGGGTGGCAAGGCAGATGGTTGAGAGATTTGGATTCAGCAAAAAGATTGGACAAATTGCGGTGGGTGCCTCTGGTGGCAATCCCTTCTTAGGGCAACAGATGTCGTCCCAAAAGGACTACTCCATGGCAACTGCTGATATTGTGGATGGAGAGGTTAGGGAATTGGTAGAGACAGCTTATTCGAGGGCCACACAGATCATCACAACTCACATCGATATCCTCCACAAGCTTGCCCAGCTCCTCATAGAGAAGGAAACTGTTGATGGTGAAGAATTCATGAGCCTCTTCATTGATGGAAAGGCCGAGCTATACGTTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Peptidase_M41

Protein Analysis

722

Amino Acids

77.02

Weight (kDa)

6.22

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 303 - 435 2.9e-45 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 458 - 500 2.1e-16 AAA+ lid domain
Peptidase_M41 PF01434 517 - 709 1.5e-71 Peptidase family M41
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50250 AT5G42270
fragaria_vesca FvH4_2g37610 FvH4_2g37610 FvH4_2g37610 FvH4_2g37610
malus_domestica MD08G1003700.v1.1 MD15G1003200.v1.1
prunus_persica Prupe.1G355600_v2.0.a1 Prupe.1G355600_v2.0.a1
pyrus_communis pycom15g00230
rosa_chinensis RchiOBHm_Chr6g0304121
rosa_laevigata RLG00000011013
rosa_multiflora Rmu_ssc0000218.1_g000001 Rmu_ssc0000218.1_g000004
rosa_roxburghii Rroxscaffold_7G00164050
rosa_rugosa Rorug06G0327800
rosa_samantha Rh6BG461000
rosa_wichuraiana Rw6G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1330
Acc36I ACCTGC 1 cut(s) 1330
Acc65I GGTACC 1 cut(s) 929
AccB1I GGYRCC 4 cut(s) 887, 929, 1418, 1910
AccBSI CCGCTC 1 cut(s) 1516
AccI GTMKAC 1 cut(s) 1302
AccII CGCG 4 cut(s) 528, 954, 995, 1130
AclWI GGATC 2 cut(s) 1526, 1625
AcoI YGGCCR 4 cut(s) 520, 573, 960, 1663
AcsI RAATTY 2 cut(s) 764, 2117
AcuI CTGAAG 2 cut(s) 485, 1578
AcyI GRCGYC 3 cut(s) 585, 1034, 1419
AfaI GTAC 3 cut(s) 875, 931, 1724
AfiI CCNNNNNNNGG 9 cut(s) 138, 388, 661, 785, 932, 1305, 1591, 1934, 1935
AgsI TTSAA 5 cut(s) 166, 193, 631, 859, 1845
AjiI CACGTC 1 cut(s) 1469
AjnI CCWGG 5 cut(s) 387, 709, 718, 925, 1737
AjuI GAANNNNNNNTTGG 6 cut(s) 146, 178, 749, 781, 1776, 1808
Alw21I GWGCWC 2 cut(s) 309, 1347
Alw26I GTCTC 6 cut(s) 604, 776, 1558, 1682, 1699, 2009
Alw44I GTGCAC 1 cut(s) 1343
AlwI GGATC 2 cut(s) 1526, 1625
AlwNI CAGNNNCTG 2 cut(s) 1180, 1435
ApaLI GTGCAC 1 cut(s) 1343
ApeKI GCWGC 5 cut(s) 414, 835, 1177, 1448, 1460
ApoI RAATTY 2 cut(s) 764, 2117
ArsI GACNNNNNNTTYG 2 cut(s) 2008, 2040
AseI ATTAAT 1 cut(s) 1170
Asp718I GGTACC 1 cut(s) 929
AspLEI GCGC 8 cut(s) 426, 530, 956, 995, 1078, 1130, 1363, 1421
AspS9I GGNCC 9 cut(s) 707, 716, 734, 753, 920, 975, 1272, 1529, 2031
AsuC2I CCSGG 2 cut(s) 1275, 1407
AsuHPI GGTGA 5 cut(s) 385, 802, 1306, 1672, 2123
AvaII GGWCC 4 cut(s) 753, 920, 975, 1529
BaeGI GKGCMC 1 cut(s) 1347
BalI TGGCCA 2 cut(s) 575, 1665
BanI GGYRCC 4 cut(s) 887, 929, 1418, 1910
BauI CACGAG 2 cut(s) 988, 1659
BbsI GAAGAC 1 cut(s) 1566
Bbv12I GWGCWC 2 cut(s) 309, 1347
BbvI GCAGC 5 cut(s) 426, 822, 1164, 1435, 1472
BccI CCATC 9 cut(s) 512, 1129, 1183, 1187, 1658, 1855, 1985, 2102, 2132
BceAI ACGGC 5 cut(s) 431, 497, 598, 941, 1063
BciT130I CCWGG 5 cut(s) 389, 711, 720, 927, 1739
BcnI CCSGG 2 cut(s) 1275, 1407
BcoDI GTCTC 6 cut(s) 604, 776, 1558, 1682, 1699, 2009
BfaI CTAG 2 cut(s) 260, 1350
BfmI CTRYAG 4 cut(s) 427, 836, 1337, 1427
BfoI RGCGCY 4 cut(s) 427, 1079, 1364, 1422
BfuAI ACCTGC 1 cut(s) 1330
BglI GCCNNNNNGGC 3 cut(s) 724, 968, 1671
Bme1390I CCNGG 7 cut(s) 389, 711, 720, 927, 1275, 1407, 1739
Bme18I GGWCC 4 cut(s) 753, 920, 975, 1529
BmgBI CACGTC 1 cut(s) 1469
BmgT120I GGNCC 9 cut(s) 707, 716, 734, 753, 920, 975, 1272, 1529, 2031
BmiI GGNNCC 9 cut(s) 46, 736, 889, 921, 931, 976, 977, 1420, 1912
BmrFI CCNGG 7 cut(s) 389, 711, 720, 927, 1275, 1407, 1739
BmsI GCATC 4 cut(s) 1096, 1495, 1608, 1829
BoxI GACNNNNGTC 1 cut(s) 1711
BpiI GAAGAC 1 cut(s) 1566
BpmI CTGGAG 4 cut(s) 720, 831, 1532, 1760
Bpu10I CCTNAGC 2 cut(s) 375, 1368
BpuEI CTTGAG 1 cut(s) 1730
BpuMI CCSGG 2 cut(s) 1275, 1407
Bsa29I ATCGAT 2 cut(s) 1095, 2058
BsaBI GATNNNNATC 1 cut(s) 1649
BsaHI GRCGYC 3 cut(s) 585, 1034, 1419
BsaI GGTCTC 1 cut(s) 1682
BsaJI CCNNGG 7 cut(s) 387, 709, 738, 883, 957, 1762, 1968
BsaWI WCCGGW 1 cut(s) 1211
BsaXI ACNNNNNCTCC 8 cut(s) 265, 271, 277, 291, 295, 301, 307, 321
Bsc4I CCNNNNNNNGG 9 cut(s) 138, 388, 661, 785, 932, 1305, 1591, 1934, 1935
Bse118I RCCGGY 1 cut(s) 932
Bse1I ACTGG 1 cut(s) 1420
Bse3DI GCAATG 1 cut(s) 1521
Bse8I GATNNNNATC 1 cut(s) 1649
BseBI CCWGG 5 cut(s) 389, 711, 720, 927, 1739
BseCI ATCGAT 2 cut(s) 1095, 2058
BseDI CCNNGG 7 cut(s) 387, 709, 738, 883, 957, 1762, 1968
BseGI GGATG 9 cut(s) 101, 911, 1140, 1198, 1252, 1378, 1498, 1653, 1996
BseJI GATNNNNATC 1 cut(s) 1649
BseLI CCNNNNNNNGG 9 cut(s) 138, 388, 661, 785, 932, 1305, 1591, 1934, 1935
BseMI GCAATG 1 cut(s) 1521
BseNI ACTGG 1 cut(s) 1420
BseRI GAGGAG 7 cut(s) 57, 328, 350, 353, 717, 1411, 2075
BseSI GKGCMC 1 cut(s) 1347
BseXI GCAGC 5 cut(s) 426, 822, 1164, 1435, 1472
BseYI CCCAGC 5 cut(s) 132, 315, 727, 971, 2079
Bsh1236I CGCG 4 cut(s) 528, 954, 995, 1130
BshNI GGYRCC 4 cut(s) 887, 929, 1418, 1910
BshVI ATCGAT 2 cut(s) 1095, 2058
BsiHKAI GWGCWC 2 cut(s) 309, 1347
BsiSI CCGG 7 cut(s) 672, 933, 963, 1212, 1244, 1275, 1407
BslFI GGGAC 4 cut(s) 527, 988, 1061, 1936
BslI CCNNNNNNNGG 9 cut(s) 138, 388, 661, 785, 932, 1305, 1591, 1934, 1935
BsmAI GTCTC 6 cut(s) 604, 776, 1558, 1682, 1699, 2009
BsmBI CGTCTC 2 cut(s) 604, 776
BsmFI GGGAC 4 cut(s) 527, 988, 1061, 1936
Bso31I GGTCTC 1 cut(s) 1682
Bsp1286I GDGCHC 2 cut(s) 309, 1347
Bsp1407I TGTACA 1 cut(s) 1722
Bsp143I GATC 5 cut(s) 1423, 1497, 1518, 1630, 2040
Bsp19I CCATGG 2 cut(s) 738, 1968
BspDI ATCGAT 2 cut(s) 1095, 2058
BspFNI CGCG 4 cut(s) 528, 954, 995, 1130
BspHI TCATGA 1 cut(s) 2121
BspLI GGNNCC 9 cut(s) 46, 736, 889, 921, 931, 976, 977, 1420, 1912
BspMAI CTGCAG 3 cut(s) 840, 1341, 1431
BspMI ACCTGC 1 cut(s) 1330
BspPI GGATC 2 cut(s) 1526, 1625
BspQI GCTCTTC 1 cut(s) 236
BspT107I GGYRCC 4 cut(s) 887, 929, 1418, 1910
BspTNI GGTCTC 1 cut(s) 1682
BsrBI CCGCTC 1 cut(s) 1516
BsrDI GCAATG 1 cut(s) 1521
BsrFI RCCGGY 1 cut(s) 932
BsrGI TGTACA 1 cut(s) 1722
BsrI ACTGG 1 cut(s) 1420
BssAI RCCGGY 1 cut(s) 932
BssECI CCNNGG 7 cut(s) 387, 709, 738, 883, 957, 1762, 1968
BssMI GATC 5 cut(s) 1423, 1497, 1518, 1630, 2040
BssNI GRCGYC 3 cut(s) 585, 1034, 1419
BssSI CACGAG 2 cut(s) 988, 1659
BssT1I CCWWGG 2 cut(s) 738, 1968
Bst2BI CACGAG 2 cut(s) 988, 1659
Bst2UI CCWGG 5 cut(s) 389, 711, 720, 927, 1739
Bst4CI ACNGT 5 cut(s) 491, 1300, 1722, 1727, 2104
Bst6I CTCTTC 5 cut(s) 34, 236, 241, 1695, 2135
BstACI GRCGYC 3 cut(s) 585, 1034, 1419
BstAUI TGTACA 1 cut(s) 1722
BstC8I GCNNGC 8 cut(s) 696, 725, 952, 1321, 1365, 1599, 1672, 2076
BstDEI CTNAG 4 cut(s) 375, 1368, 1641, 1933
BstDSI CCRYGG 3 cut(s) 738, 957, 1968
BstEII GGTNACC 2 cut(s) 391, 1294
BstF5I GGATG 9 cut(s) 101, 911, 1140, 1198, 1252, 1378, 1498, 1653, 1996
BstFNI CGCG 4 cut(s) 528, 954, 995, 1130
BstH2I RGCGCY 4 cut(s) 427, 1079, 1364, 1422
BstHHI GCGC 8 cut(s) 426, 530, 956, 995, 1078, 1130, 1363, 1421
BstKTI GATC 5 cut(s) 1426, 1500, 1521, 1633, 2043
BstMAI GTCTC 6 cut(s) 604, 776, 1558, 1682, 1699, 2009
BstMBI GATC 5 cut(s) 1423, 1497, 1518, 1630, 2040
BstNI CCWGG 5 cut(s) 389, 711, 720, 927, 1739
BstNSI RCATGY 1 cut(s) 1601
BstPAI GACNNNNGTC 1 cut(s) 1711
BstPI GGTNACC 2 cut(s) 391, 1294
BstSCI CCNGG 7 cut(s) 387, 709, 718, 925, 1273, 1405, 1737
BstSFI CTRYAG 4 cut(s) 427, 836, 1337, 1427
BstSLI GKGCMC 1 cut(s) 1347
BstUI CGCG 4 cut(s) 528, 954, 995, 1130
BstV1I GCAGC 5 cut(s) 426, 822, 1164, 1435, 1472
BstV2I GAAGAC 1 cut(s) 1566
Bsu15I ATCGAT 2 cut(s) 1095, 2058
BsuTUI ATCGAT 2 cut(s) 1095, 2058
BtgI CCRYGG 3 cut(s) 738, 957, 1968
BtrI CACGTC 1 cut(s) 1469
BtsCI GGATG 9 cut(s) 101, 911, 1140, 1198, 1252, 1378, 1498, 1653, 1996
BtsIMutI CAGTG 2 cut(s) 396, 1413
BveI ACCTGC 1 cut(s) 1330
Cac8I GCNNGC 8 cut(s) 696, 725, 952, 1321, 1365, 1599, 1672, 2076
CaiI CAGNNNCTG 2 cut(s) 1180, 1435
CciI TCATGA 1 cut(s) 2121
CfoI GCGC 8 cut(s) 426, 530, 956, 995, 1078, 1130, 1363, 1421
Cfr10I RCCGGY 1 cut(s) 932
Cfr13I GGNCC 9 cut(s) 707, 716, 734, 753, 920, 975, 1272, 1529, 2031
ClaI ATCGAT 2 cut(s) 1095, 2058
CseI GACGC 2 cut(s) 574, 1023
CsiI ACCWGGT 1 cut(s) 925
Csp6I GTAC 3 cut(s) 874, 930, 1723
CviAII CATG 7 cut(s) 577, 739, 1586, 1598, 1834, 1969, 2122
CviQI GTAC 3 cut(s) 874, 930, 1723
DdeI CTNAG 4 cut(s) 375, 1368, 1641, 1933
DinI GGCGCC 1 cut(s) 1420
DpnI GATC 5 cut(s) 1425, 1499, 1520, 1632, 2042
DpnII GATC 5 cut(s) 1423, 1497, 1518, 1630, 2040
EaeI YGGCCR 4 cut(s) 520, 573, 960, 1663
Eam1104I CTCTTC 5 cut(s) 34, 236, 241, 1695, 2135
EarI CTCTTC 5 cut(s) 34, 236, 241, 1695, 2135
EciI GGCGGA 1 cut(s) 285
Eco130I CCWWGG 2 cut(s) 738, 1968
Eco147I AGGCCT 1 cut(s) 1309
Eco31I GGTCTC 1 cut(s) 1682
Eco32I GATATC 2 cut(s) 1647, 2062
Eco47I GGWCC 4 cut(s) 753, 920, 975, 1529
Eco57I CTGAAG 2 cut(s) 485, 1578
Eco91I GGTNACC 2 cut(s) 391, 1294
EcoO109I RGGNCCY 1 cut(s) 975
EcoO65I GGTNACC 2 cut(s) 391, 1294
EcoRI GAATTC 1 cut(s) 2117
EcoRII CCWGG 5 cut(s) 387, 709, 718, 925, 1737
EcoRV GATATC 2 cut(s) 1647, 2062
EcoT14I CCWWGG 2 cut(s) 738, 1968
EgeI GGCGCC 1 cut(s) 1420
EheI GGCGCC 1 cut(s) 1420
ErhI CCWWGG 2 cut(s) 738, 1968
Esp3I CGTCTC 2 cut(s) 604, 776
FaeI CATG 7 cut(s) 580, 742, 1589, 1601, 1837, 1972, 2125
FalI AAGNNNNNCTT 4 cut(s) 894, 926, 1692, 1724
FaqI GGGAC 4 cut(s) 527, 988, 1061, 1936
FatI CATG 7 cut(s) 576, 738, 1585, 1597, 1833, 1968, 2121
FauI CCCGC 6 cut(s) 262, 716, 1038, 1123, 1136, 1585
FblI GTMKAC 1 cut(s) 1302
FokI GGATG 9 cut(s) 88, 918, 1147, 1205, 1259, 1385, 1505, 1640, 2003
FspBI CTAG 2 cut(s) 260, 1350
GlaI GCGC 8 cut(s) 425, 529, 955, 994, 1077, 1129, 1362, 1420
GsaI CCCAGC 5 cut(s) 136, 319, 731, 975, 2083
GsuI CTGGAG 4 cut(s) 720, 831, 1532, 1760
HaeII RGCGCY 4 cut(s) 427, 1079, 1364, 1422
HapII CCGG 7 cut(s) 672, 933, 963, 1212, 1244, 1275, 1407
HgaI GACGC 2 cut(s) 574, 1023
HhaI GCGC 8 cut(s) 426, 530, 956, 995, 1078, 1130, 1363, 1421
Hin1I GRCGYC 3 cut(s) 585, 1034, 1419
Hin1II CATG 7 cut(s) 580, 742, 1589, 1601, 1837, 1972, 2125
Hin6I GCGC 8 cut(s) 424, 528, 954, 993, 1076, 1128, 1361, 1419
HinP1I GCGC 8 cut(s) 424, 528, 954, 993, 1076, 1128, 1361, 1419
HincII GTYRAC 1 cut(s) 1303
HindII GTYRAC 1 cut(s) 1303
HindIII AAGCTT 1 cut(s) 2072
HinfI GANTC 7 cut(s) 179, 471, 611, 1256, 1712, 1744, 1878
HpaII CCGG 7 cut(s) 672, 933, 963, 1212, 1244, 1275, 1407
HphI GGTGA 5 cut(s) 385, 802, 1306, 1672, 2123
Hpy166II GTNNAC 5 cut(s) 876, 923, 1303, 1345, 1378
Hpy188I TCNGA 6 cut(s) 44, 604, 749, 802, 1003, 1558
Hpy8I GTNNAC 5 cut(s) 876, 923, 1303, 1345, 1378
Hpy99I CGWCG 5 cut(s) 518, 539, 590, 1039, 1304
HpyCH4III ACNGT 5 cut(s) 491, 1300, 1722, 1727, 2104
HpyCH4IV ACGT 2 cut(s) 1468, 2157
HpyCH4V TGCA 7 cut(s) 838, 1083, 1339, 1345, 1429, 1613, 1837
HpyF3I CTNAG 4 cut(s) 375, 1368, 1641, 1933
HpySE526I ACGT 2 cut(s) 1468, 2157
Hsp92I GRCGYC 3 cut(s) 585, 1034, 1419
Hsp92II CATG 7 cut(s) 580, 742, 1589, 1601, 1837, 1972, 2125
HspAI GCGC 8 cut(s) 424, 528, 954, 993, 1076, 1128, 1361, 1419
KasI GGCGCC 1 cut(s) 1418
KflI GGGWCCC 1 cut(s) 975
KpnI GGTACC 1 cut(s) 933
Kzo9I GATC 5 cut(s) 1423, 1497, 1518, 1630, 2040
LguI GCTCTTC 1 cut(s) 236
LmnI GCTCC 7 cut(s) 312, 812, 832, 1159, 1513, 1696, 2088
Lsp1109I GCAGC 5 cut(s) 426, 822, 1164, 1435, 1472
LweI GCATC 4 cut(s) 1096, 1495, 1608, 1829
MabI ACCWGGT 1 cut(s) 925
MaeI CTAG 2 cut(s) 260, 1350
MaeII ACGT 2 cut(s) 1468, 2157
MaeIII GTNAC 5 cut(s) 391, 790, 1294, 1469, 1807
MalI GATC 5 cut(s) 1425, 1499, 1520, 1632, 2042
MbiI CCGCTC 1 cut(s) 1516
MboI GATC 5 cut(s) 1423, 1497, 1518, 1630, 2040
MhlI GDGCHC 2 cut(s) 309, 1347
MlsI TGGCCA 2 cut(s) 575, 1665
MluNI TGGCCA 2 cut(s) 575, 1665
Mly113I GGCGCC 1 cut(s) 1419
MlyI GAGTC 3 cut(s) 480, 1250, 1721
MmeI TCCRAC 1 cut(s) 2140
Mox20I TGGCCA 2 cut(s) 575, 1665
MscI TGGCCA 2 cut(s) 575, 1665
MseI TTAA 2 cut(s) 1170, 1329
Msp20I TGGCCA 2 cut(s) 575, 1665
MspA1I CMGCKG 1 cut(s) 1177
MspI CCGG 7 cut(s) 672, 933, 963, 1212, 1244, 1275, 1407
MspR9I CCNGG 7 cut(s) 389, 711, 720, 927, 1275, 1407, 1739
MvaI CCWGG 5 cut(s) 389, 711, 720, 927, 1739
MvnI CGCG 4 cut(s) 528, 954, 995, 1130
NarI GGCGCC 1 cut(s) 1419
NciI CCSGG 2 cut(s) 1275, 1407
NcoI CCATGG 2 cut(s) 738, 1968
NdeII GATC 5 cut(s) 1423, 1497, 1518, 1630, 2040
NlaIII CATG 7 cut(s) 580, 742, 1589, 1601, 1837, 1972, 2125
NlaIV GGNNCC 9 cut(s) 46, 736, 889, 921, 931, 976, 977, 1420, 1912
NmeAIII GCCGAG 1 cut(s) 1238
NmuCI GTSAC 5 cut(s) 391, 790, 1294, 1469, 1807
NspI RCATGY 1 cut(s) 1601
PaeI GCATGC 1 cut(s) 1601
PagI TCATGA 1 cut(s) 2121
PaqCI CACCTGC 1 cut(s) 1330
PceI AGGCCT 1 cut(s) 1309
PciSI GCTCTTC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 2 cut(s) 594, 1076
PfeI GAWTC 4 cut(s) 179, 611, 1744, 1878
PleI GAGTC 3 cut(s) 479, 1250, 1720
PluTI GGCGCC 1 cut(s) 1422
PpsI GAGTC 3 cut(s) 479, 1250, 1720
PpuMI RGGWCCY 1 cut(s) 975
PshAI GACNNNNGTC 1 cut(s) 1711
PshBI ATTAAT 1 cut(s) 1170
Psp5II RGGWCCY 1 cut(s) 975
Psp6I CCWGG 5 cut(s) 387, 709, 718, 925, 1737
PspEI GGTNACC 2 cut(s) 391, 1294
PspFI CCCAGC 5 cut(s) 132, 315, 727, 971, 2079
PspGI CCWGG 5 cut(s) 387, 709, 718, 925, 1737
PspN4I GGNNCC 9 cut(s) 46, 736, 889, 921, 931, 976, 977, 1420, 1912
PspPI GGNCC 9 cut(s) 707, 716, 734, 753, 920, 975, 1272, 1529, 2031
PspPPI RGGWCCY 1 cut(s) 975
PstI CTGCAG 3 cut(s) 840, 1341, 1431
PstNI CAGNNNCTG 2 cut(s) 1180, 1435
PvuII CAGCTG 1 cut(s) 1177
RsaI GTAC 3 cut(s) 875, 931, 1724
RsaNI GTAC 3 cut(s) 874, 930, 1723
SalI GTCGAC 1 cut(s) 1301
SapI GCTCTTC 1 cut(s) 236
SaqAI TTAA 2 cut(s) 1170, 1329
Sau3AI GATC 5 cut(s) 1423, 1497, 1518, 1630, 2040
Sau96I GGNCC 9 cut(s) 707, 716, 734, 753, 920, 975, 1272, 1529, 2031
SchI GAGTC 3 cut(s) 480, 1250, 1721
ScrFI CCNGG 7 cut(s) 389, 711, 720, 927, 1275, 1407, 1739
SduI GDGCHC 2 cut(s) 309, 1347
SexAI ACCWGGT 1 cut(s) 925
SfaNI GCATC 4 cut(s) 1096, 1495, 1608, 1829
SfcI CTRYAG 4 cut(s) 427, 836, 1337, 1427
SfoI GGCGCC 1 cut(s) 1420
SinI GGWCC 4 cut(s) 753, 920, 975, 1529
SmlI CTYRAG 1 cut(s) 1709
SmoI CTYRAG 1 cut(s) 1709
SphI GCATGC 1 cut(s) 1601
SseBI AGGCCT 1 cut(s) 1309
SspDI GGCGCC 1 cut(s) 1418
SspMI CTAG 2 cut(s) 260, 1350
StuI AGGCCT 1 cut(s) 1309
StyD4I CCNGG 7 cut(s) 387, 709, 718, 925, 1273, 1405, 1737
StyI CCWWGG 2 cut(s) 738, 1968
TaaI ACNGT 5 cut(s) 491, 1300, 1722, 1727, 2104
TaiI ACGT 2 cut(s) 1471, 2160
TaqII GACCGA 1 cut(s) 1302
TatI WGTACW 2 cut(s) 873, 1722
TauI GCSGC 7 cut(s) 224, 525, 528, 735, 998, 1143, 1232
TfiI GAWTC 4 cut(s) 179, 611, 1744, 1878
Tru1I TTAA 2 cut(s) 1170, 1329
Tru9I TTAA 2 cut(s) 1170, 1329
TscAI CASTG 2 cut(s) 403, 1420
TseFI GTSAC 5 cut(s) 391, 790, 1294, 1469, 1807
TseI GCWGC 5 cut(s) 414, 835, 1177, 1448, 1460
Tsp45I GTSAC 5 cut(s) 391, 790, 1294, 1469, 1807
TspDTI ATGAA 7 cut(s) 593, 625, 1081, 1454, 1822, 2110, 2122
TspGWI ACGGA 3 cut(s) 523, 586, 1199
TspRI CASTG 2 cut(s) 403, 1420
VneI GTGCAC 1 cut(s) 1343
VpaK11BI GGWCC 4 cut(s) 753, 920, 975, 1529
VspI ATTAAT 1 cut(s) 1170
XapI RAATTY 2 cut(s) 764, 2117
XbaI TCTAGA 1 cut(s) 1349
XceI RCATGY 1 cut(s) 1601
XmiI GTMKAC 1 cut(s) 1302
XspI CTAG 2 cut(s) 260, 1350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.