pycom15g01130

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011843
Physical Location & Seq
Forward (+)
601054 .. 602760
1707 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
ATGGAGAAAGAAGTAGGAGGAGGAGGAATGCAAGTGACGAGGACAAGGAGGGCGGAGATCGATACAAGGCAACCGTTCCGCTCCGTCAAAGAGGCAGTTGCGTTGTTCGGAGAAAAAGTTCTTGCCGGAGAGCTCTATGCCTCCCAGCTCAAACAGATGCAGAACGAAGCAAATGAAAATAGGCATGGCCCCGCATTATCCAGAATTGGAACTGTGAGAGCAGAACTAGAGGACACAAAACAAAGCCTCCAAAAAGCCAGACAAGAAAGTGAGCTCATGGAAAACTGCCTCTCTTCTCTAAAACAGGAGCTCGAACGCACAAAGCGAGAGCTCCAGCAACTGAAGGAACGGGAGTATGAAAAACACTTCATCGAAACGGAGATTGAAGACGTTAGGATTGTCGACGAAGACTCAACCAAGTTTACAACCAAAACACAAGCATCTAGTGAGGAAGATGAAAGTTTTGAGTTCCAAAAGAAAAGATATGTGGCATTTGGAAATCCACCTTCCGTGTCTCAAGTGATGATTGCACAAGGTGGTGTTCATGAAACATTGGAAAGACACCATTCTCTCACGAAAAAGAAGAAGAAGCCCATGATTCCTCTGATCGCCGGGATCTTTTCTAAGAAGAAAGGAAGCTCACAGGCTGCTTACGCTTGA

Protein Analysis

220

Amino Acids

24.92

Weight (kDa)

8.5

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 46 - 121 4.6e-07 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75720 AT1G75720 AT1G75720
fragaria_vesca FvH4_2g38540
malus_domestica MD08G1013500.v1.1 MD15G1013000.v1.1
prunus_persica Prupe.1G366100_v2.0.a1
pyrus_communis pycom08g01190 pycom15g01130
rosa_chinensis RchiOBHm_Chr6g0302771
rosa_laevigata RLG00000011139
rosa_roxburghii Rroxscaffold_7G00165340
rosa_rugosa Rorug06G0316700 Rorug06G0316800
rosa_samantha Rh6AG430200 Rh6BG472400 Rh6CG442700 Rh6DG429400
rosa_wichuraiana Rw6G037260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 402
AciI CCGC 3 cut(s) 53, 79, 192
AclWI GGATC 1 cut(s) 623
AcuI CTGAAG 1 cut(s) 362
AdeI CACNNNGTG 1 cut(s) 536
AgsI TTSAA 1 cut(s) 386
AloI GAACNNNNNNTCC 2 cut(s) 102, 134
AluBI AGCT 6 cut(s) 133, 148, 274, 310, 331, 639
AluI AGCT 6 cut(s) 133, 148, 274, 310, 331, 639
Alw21I GWGCWC 4 cut(s) 135, 276, 312, 333
Alw26I GTCTC 1 cut(s) 519
AlwI GGATC 1 cut(s) 623
AlwNI CAGNNNCTG 1 cut(s) 340
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 1 cut(s) 647
Asp700I GAANNNNTTC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 188
AsuC2I CCSGG 1 cut(s) 613
BanII GRGCYC 4 cut(s) 135, 276, 312, 333
BbsI GAAGAC 2 cut(s) 393, 414
Bbv12I GWGCWC 4 cut(s) 135, 276, 312, 333
BbvI GCAGC 1 cut(s) 634
BcnI CCSGG 1 cut(s) 613
BcoDI GTCTC 1 cut(s) 519
BfaI CTAG 2 cut(s) 227, 444
BisI GCNGC 1 cut(s) 648
BlsI GCNGC 1 cut(s) 649
Bme1390I CCNGG 1 cut(s) 613
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 613
BmsI GCATC 2 cut(s) 147, 449
BpiI GAAGAC 2 cut(s) 393, 414
BpmI CTGGAG 1 cut(s) 317
BpuEI CTTGAG 1 cut(s) 501
BpuMI CCSGG 1 cut(s) 613
Bsa29I ATCGAT 1 cut(s) 60
BsaXI ACNNNNNCTCC 4 cut(s) 102, 132, 231, 261
BseCI ATCGAT 1 cut(s) 60
BseRI GAGGAG 2 cut(s) 33, 36
BseXI GCAGC 1 cut(s) 634
BseYI CCCAGC 1 cut(s) 144
BshFI GGCC 1 cut(s) 189
BshVI ATCGAT 1 cut(s) 60
BsiHKAI GWGCWC 4 cut(s) 135, 276, 312, 333
BsiSI CCGG 2 cut(s) 126, 612
BsmAI GTCTC 1 cut(s) 519
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 1 cut(s) 189
Bsp1286I GDGCHC 4 cut(s) 135, 276, 312, 333
Bsp143I GATC 3 cut(s) 57, 606, 615
BspACI CCGC 3 cut(s) 53, 79, 192
BspANI GGCC 1 cut(s) 189
BspDI ATCGAT 1 cut(s) 60
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 1 cut(s) 623
BsrBI CCGCTC 1 cut(s) 81
BssMI GATC 3 cut(s) 57, 606, 615
Bst4CI ACNGT 2 cut(s) 75, 214
Bst6I CTCTTC 1 cut(s) 298
BstDEI CTNAG 1 cut(s) 624
BstKTI GATC 3 cut(s) 60, 609, 618
BstMAI GTCTC 1 cut(s) 519
BstMBI GATC 3 cut(s) 57, 606, 615
BstMWI GCNNNNNNNGC 1 cut(s) 653
BstSCI CCNGG 1 cut(s) 611
BstV1I GCAGC 1 cut(s) 634
BstV2I GAAGAC 2 cut(s) 393, 414
BstX2I RGATCY 1 cut(s) 615
BstYI RGATCY 1 cut(s) 615
Bsu15I ATCGAT 1 cut(s) 60
BsuRI GGCC 1 cut(s) 189
BsuTUI ATCGAT 1 cut(s) 60
CaiI CAGNNNCTG 1 cut(s) 340
CciI TCATGA 1 cut(s) 544
Cfr13I GGNCC 1 cut(s) 188
ClaI ATCGAT 1 cut(s) 60
CviAII CATG 4 cut(s) 185, 277, 545, 595
DdeI CTNAG 1 cut(s) 624
DpnI GATC 3 cut(s) 59, 608, 617
DpnII GATC 3 cut(s) 57, 606, 615
DraIII CACNNNGTG 1 cut(s) 536
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 4 cut(s) 133, 274, 310, 331
Eco24I GRGCYC 4 cut(s) 135, 276, 312, 333
Eco53kI GAGCTC 4 cut(s) 133, 274, 310, 331
Eco57I CTGAAG 1 cut(s) 362
EcoICRI GAGCTC 4 cut(s) 133, 274, 310, 331
EcoT38I GRGCYC 4 cut(s) 135, 276, 312, 333
FaeI CATG 4 cut(s) 188, 280, 548, 598
FaiI YATR 7 cut(s) 138, 186, 278, 357, 486, 546, 596
FatI CATG 4 cut(s) 184, 276, 544, 594
FauI CCCGC 1 cut(s) 199
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 1 cut(s) 648
FriOI GRGCYC 4 cut(s) 135, 276, 312, 333
Fsp4HI GCNGC 1 cut(s) 648
FspBI CTAG 2 cut(s) 227, 444
GluI GCNGC 1 cut(s) 648
GsaI CCCAGC 1 cut(s) 148
GsuI CTGGAG 1 cut(s) 317
HaeIII GGCC 1 cut(s) 189
HapII CCGG 2 cut(s) 126, 612
Hin1II CATG 4 cut(s) 188, 280, 548, 598
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 2 cut(s) 410, 598
HpaII CCGG 2 cut(s) 126, 612
Hpy166II GTNNAC 2 cut(s) 403, 423
Hpy188I TCNGA 2 cut(s) 110, 606
Hpy188III TCNNGA 3 cut(s) 201, 545, 574
Hpy8I GTNNAC 2 cut(s) 403, 423
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 2 cut(s) 337, 516
HpyCH4III ACNGT 2 cut(s) 75, 214
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 3 cut(s) 31, 160, 530
HpyF10VI GCNNNNNNNGC 1 cut(s) 653
HpyF3I CTNAG 1 cut(s) 624
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 4 cut(s) 188, 280, 548, 598
Kzo9I GATC 3 cut(s) 57, 606, 615
LmnI GCTCC 3 cut(s) 86, 307, 336
LpnPI CCDG 8 cut(s) 139, 158, 214, 271, 290, 347, 625, 629
Lsp1109I GCAGC 1 cut(s) 634
LweI GCATC 2 cut(s) 147, 449
MaeI CTAG 2 cut(s) 227, 444
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 1 cut(s) 34
MalI GATC 3 cut(s) 59, 608, 617
MbiI CCGCTC 1 cut(s) 81
MboI GATC 3 cut(s) 57, 606, 615
MboII GAAGA 7 cut(s) 285, 398, 419, 464, 595, 598, 640
MflI RGATCY 1 cut(s) 615
MhlI GDGCHC 4 cut(s) 135, 276, 312, 333
MluCI AATT 1 cut(s) 204
MlyI GAGTC 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 117
MspI CCGG 2 cut(s) 126, 612
MspR9I CCNGG 1 cut(s) 613
Mva1269I GAATGC 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 653
NciI CCSGG 1 cut(s) 613
NdeII GATC 3 cut(s) 57, 606, 615
NlaIII CATG 4 cut(s) 188, 280, 548, 598
NlaIV GGNNCC 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 34
PagI TCATGA 1 cut(s) 544
PcsI WCGNNNNNNNCGW 1 cut(s) 322
PctI GAATGC 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 598
PkrI GCNGC 1 cut(s) 649
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
Psp124BI GAGCTC 4 cut(s) 135, 276, 312, 333
PspFI CCCAGC 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 1 cut(s) 188
PstNI CAGNNNCTG 1 cut(s) 340
PsuI RGATCY 1 cut(s) 615
SacI GAGCTC 4 cut(s) 135, 276, 312, 333
SalI GTCGAC 1 cut(s) 401
SatI GCNGC 1 cut(s) 648
Sau3AI GATC 3 cut(s) 57, 606, 615
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 613
SduI GDGCHC 4 cut(s) 135, 276, 312, 333
SetI ASST 9 cut(s) 135, 150, 276, 312, 333, 393, 508, 538, 641
SfaNI GCATC 2 cut(s) 147, 449
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 1 cut(s) 204
SsiI CCGC 3 cut(s) 53, 79, 192
SspMI CTAG 2 cut(s) 227, 444
SstI GAGCTC 4 cut(s) 135, 276, 312, 333
StyD4I CCNGG 1 cut(s) 611
TaaI ACNGT 2 cut(s) 75, 214
TaiI ACGT 1 cut(s) 393
TaqI TCGA 4 cut(s) 60, 312, 372, 402
TasI AATT 1 cut(s) 204
TfiI GAWTC 1 cut(s) 598
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 1 cut(s) 647
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 6 cut(s) 189, 358, 372, 471, 533, 561
TspGWI ACGGA 3 cut(s) 73, 392, 499
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 2 cut(s) 227, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.