pycom15g01740

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009802
Physical Location & Seq
Reverse (-)
1000747 .. 1005380
4634 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2559 bp
ATGGAAACTCGCTCGGTTTCCAAGCTCTTAGTCTTGTTCATGTCGATGGCATTGTTCTTGGGTTCTGAGCTCATCCACTGCACCACCGTCACCTACGACAAGAAGGCCATTCTCATCGATGGCCAGAGAAGATTGCTCATTTCTGGATCCATTCATTATCCCAGAAGCACCCCTGAAATGTGGGAAGGCCTCATACAGAAAGCAAAAGATGGAGGCTTGGATGTTATCGACACCTACGTTTTCTGGAACGGACATGAACCTTCTCCTGGCAATTATTATTTTGAGGGGAGATACGATCTAGTAGGGTTCATAAAGACAGTAGAGAAAGCAGGGCTCCTTCTTCATCTGCGCATTGGGCCTTATGTTTGTGCAGAGTGGAATTTTGGAGGATTTCCTGTTTGGCTGAAATATGTACCCGGCATCAGTTTCAGGACGGATAATGGGCCATTTAAGATGGCAATGCAAGGGTTCACCCAGAAGATTGTCCAGATGATGAAAGATGAGAAGCTATTTGCATCACAAGGTGGTCCCATCATCCTTTCCCAGATTGAGAATGAGTATGGACCGGAGAGCAAGGCATTGGGAGCTGCTGGCCATAACTACATTAACTGGGCTGCTCAAATGGCGGTTGGATTGGATACCGGAGTCCCATGGGTAATGTGCAAGGAAGACGATGCCCCAGACCCTATGATAAATGCATGCAACGGTTTTTACTGTGATGGATTCACTCCCAACAAACCTTACAAGCCTGCCATGTGGACGGAAGCTTGGAGCGGCTGGTTTACAGAATTTGGTGGCACAATTCACCATCGACCTGTCCAAGATTTAGCATTTGCAGTTGGTCGATTCATACAGAAGGGTGGCTCATATGTGAATTACTACATGTATCATGGAGGAACAAACTTCGGACGAACAGCTGGAGGCCCCTTCATTACAACCAGTTACGACTATGATGCTCCCATTGATGAATATGGTTTGATCAGAGAACCTAAATATGGTCATCTGAAGGAGCTTCACAAGGCTATCAAGCTATGTGAACATTCTTTACTCTCTTCAGAGCCTACAGTCACTTCACTAGGAGCCTATCATCAGGCTTATGTATTCAATTCAGGACCAAAAAGATGCGCAGCGTTTCTCTCAAACTTCCATTCAACAGGAGCAAGAGTGACTTTCAATAACTTGCACTATGATTTGCCTCCTTGGTCTGTTAGCATCCTTCCTGATTGCAGGAATGAAGTATTCAACACCGCAAAGGTGGGAGTTCAAACGTCACACGTGCAGATGATCCCGACGAACAGTCCGTTGTTTTCATGGCAGACTTACGATGAAGATATCTCTTCTCTAAACGATGGGTCAAGCATACCGGCTGTTGGACTTCTAGAGCAGATAAATGTTACTAGAGATACTAGTGACTATCTGTGGTACATGACGAATGTTGACATCAGTTCATCAGACCTCGGCGGAGGAAAAAAGCCTACTCTTACTGTGCAGTCAGCTGGTCATGCCCTCCATGTCTTCGTCAACGGACAGTATTCAGGTTCGGCGTTTGGAACTAGGGAGCAGAGGCAATTCACATTTGCTGAACCAGTCAACCTCCGTGCTGGAATAAACAGAATTGCACTGCTCAGCATAGCTGTTGGATTACCAAATGTTGGGTTGCATTATGAGTCATGGAAAACAGGAATCCAAGGTCCGGTTTTTCTGGATGGTCTTGGCCACGGAAAGAAAGACTTAACGTCGCAGAAGTGGTTCTACAAGGTCGGCCTTAAGGGAGAAGAAATGAATTTAGTCTCTCCAAACGGAGCCTCATCAGTTGATTGGATCCGGGGGTCACTAGCTACCCAAGCCAAGCAGATGTTGAAATGGTACAAGGCTTATTTCAATGCACCCGGAGGAAATGAGCCATTGGCGTTGGACATGAGTAGCATGGGAAAGGGACAAGTATGGATCGATCGGCAGAGCATCGGTAGATACTGGATGGCTTATTCCAAAGGTGACTGCAGTTCGTGCAGTTACATTGGGACATTCCGTCCCACGAAATGTCAACTTGGCTGTGGCCAACCAACTCAACAATGGTATCATGTTCCTCGTTCATGGTTAAAGCCGACGCAAAATTTGGTGGTAGTGTTTGAGGAACTAGGCGGAGATCCATCGAAGATTACCCTTGTGAAAAGATCAGTGACCGGTGTTTGTGGTGACTTACATGAGAACCACCCAAATGTCGAAAATTTTGATGTTGAGGGCAATGAAAATTCTAAAACGCTTCACCAAGCACAGGTTCATCTGCAGTGTGCACCAGGGCAGTCTATCTCGAGCATTAAGTTTGCAAGTTTCGGAACTCCTACTGGAACTTGTGGGAGTTTCCAGCAAGGAACGTGTCATGCGACAAACTCACACGCCGTGGTGGAGAAGAATTGTATCAGCCGCGAGAGTTGCTCGGTTGCTGTAACCAATAGCATTTTCAAGACAGATCCATGTCCGAATGTACTCAAACGGCTATCAGTTGAAGCTGTTTGTTCGACGGAAATTACAACCAATCAACCCAATTCGAGGAGATGA

Protein Analysis

853

Amino Acids

94.24

Weight (kDa)

7.11

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 341 1.2e-120 Glycosyl hydrolases family 35
GHD PF17834 364 - 419 1.3e-21 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 472 - 537 1.6e-09 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 621 - 705 1.8e-24 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 762 - 839 4.3e-22 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 350, 1126
AccB7I CCANNNNNTGG 1 cut(s) 1652
AccBSI CCGCTC 1 cut(s) 774
AccII CGCG 1 cut(s) 2427
AciI CCGC 6 cut(s) 626, 774, 1248, 1461, 2142, 2425
AclWI GGATC 8 cut(s) 141, 154, 1279, 1816, 1829, 1955, 2141, 2465
AcoI YGGCCR 4 cut(s) 121, 592, 1714, 2056
AcsI RAATTY 6 cut(s) 379, 788, 1783, 2113, 2227, 2251
AcuI CTGAAG 2 cut(s) 1025, 1038
AcvI CACGTG 1 cut(s) 1276
AdeI CACNNNGTG 2 cut(s) 524, 2401
AfaI GTAC 4 cut(s) 414, 1424, 1868, 2487
AfiI CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2275, 2550
AflII CTTAAG 1 cut(s) 1766
AflIII ACRYGT 3 cut(s) 882, 1273, 2375
AgeI ACCGGT 1 cut(s) 2183
AgsI TTSAA 9 cut(s) 1105, 1152, 1174, 1243, 1265, 1861, 1882, 2464, 2507
AhdI GACNNNNNGTC 3 cut(s) 1296, 1735, 2028
AhlI ACTAGT 1 cut(s) 1406
AjnI CCWGG 2 cut(s) 265, 2296
AjuI GAANNNNNNNTTGG 4 cut(s) 2262, 2294, 2444, 2476
Alw21I GWGCWC 2 cut(s) 72, 2296
Alw26I GTCTC 1 cut(s) 1795
Alw44I GTGCAC 1 cut(s) 2292
AlwI GGATC 8 cut(s) 141, 154, 1279, 1816, 1829, 1955, 2141, 2465
Ama87I CYCGRG 1 cut(s) 2311
ApaLI GTGCAC 1 cut(s) 2292
ApeKI GCWGC 3 cut(s) 587, 614, 1127
ApoI RAATTY 6 cut(s) 379, 788, 1783, 2113, 2227, 2251
ArsI GACNNNNNNTTYG 2 cut(s) 2098, 2130
AsiGI ACCGGT 1 cut(s) 2183
Asp700I GAANNNNTTC 1 cut(s) 1748
AspLEI GCGC 2 cut(s) 351, 1127
AspS9I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
AsuC2I CCSGG 3 cut(s) 417, 1826, 1890
AsuHPI GGTGA 6 cut(s) 82, 463, 797, 2006, 2207, 2258
AvaI CYCGRG 1 cut(s) 2311
AvaII GGWCC 4 cut(s) 527, 563, 1112, 1691
BaeGI GKGCMC 1 cut(s) 2296
BaeI ACNNNNGTAYC 4 cut(s) 630, 663, 2060, 2093
BalI TGGCCA 4 cut(s) 123, 594, 1716, 2058
BamHI GGATCC 2 cut(s) 146, 1821
BanII GRGCYC 2 cut(s) 72, 336
BarI GAAGNNNNNNTAC 4 cut(s) 177, 209, 1736, 1768
BbrPI CACGTG 1 cut(s) 1276
BbsI GAAGAC 2 cut(s) 675, 1507
Bbv12I GWGCWC 2 cut(s) 72, 2296
BbvI GCAGC 3 cut(s) 574, 601, 1139
BceAI ACGGC 2 cut(s) 2384, 2510
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 2 cut(s) 267, 2298
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 1 cut(s) 978
BcnI CCSGG 3 cut(s) 417, 1826, 1890
BcoDI GTCTC 1 cut(s) 1795
BcuI ACTAGT 1 cut(s) 1406
BfaI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
BfmI CTRYAG 3 cut(s) 1062, 1999, 2285
BfrI CTTAAG 1 cut(s) 1766
BfuI GTATCC 1 cut(s) 631
BisI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
BlpI GCTNAGC 1 cut(s) 1625
BlsI GCNGC 5 cut(s) 589, 616, 776, 1129, 2426
Bme1390I CCNGG 5 cut(s) 267, 417, 1826, 1890, 2298
Bme18I GGWCC 4 cut(s) 527, 563, 1112, 1691
BmeRI GACNNNNNGTC 3 cut(s) 1296, 1735, 2028
BmeT110I CYCGRG 1 cut(s) 2311
BmgT120I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
BmiI GGNNCC 7 cut(s) 148, 335, 529, 925, 1081, 1804, 1823
BmrFI CCNGG 5 cut(s) 267, 417, 1826, 1890, 2298
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 7 cut(s) 429, 524, 664, 943, 1112, 1221, 1971
BmuI ACTGGG 1 cut(s) 619
BpiI GAAGAC 2 cut(s) 675, 1507
BplI GAGNNNNNCTC 2 cut(s) 2420, 2452
BpmI CTGGAG 1 cut(s) 939
Bpu1102I GCTNAGC 1 cut(s) 1625
BpuMI CCSGG 3 cut(s) 417, 1826, 1890
Bsa29I ATCGAT 2 cut(s) 117, 1950
BsaAI YACGTR 1 cut(s) 1276
BsaJI CCNNGG 8 cut(s) 650, 1199, 1456, 1687, 1717, 1825, 2297, 2400
BsaWI WCCGGW 4 cut(s) 565, 641, 1693, 2183
BsaXI ACNNNNNCTCC 2 cut(s) 1149, 1179
Bsc4I CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2275, 2550
Bse118I RCCGGY 2 cut(s) 1363, 2183
Bse1I ACTGG 5 cut(s) 614, 939, 1586, 1979, 2350
Bse3DI GCAATG 2 cut(s) 465, 2251
BseBI CCWGG 2 cut(s) 267, 2298
BseCI ATCGAT 2 cut(s) 117, 1950
BseDI CCNNGG 8 cut(s) 650, 1199, 1456, 1687, 1717, 1825, 2297, 2400
BseGI GGATG 6 cut(s) 72, 226, 534, 1212, 1711, 1983
BseLI CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2275, 2550
BseMI GCAATG 2 cut(s) 465, 2251
BseMII CTCAG 2 cut(s) 57, 1639
BseNI ACTGG 5 cut(s) 614, 939, 1586, 1979, 2350
BseSI GKGCMC 1 cut(s) 2296
BseXI GCAGC 3 cut(s) 574, 601, 1139
BsgI GTGCAG 5 cut(s) 64, 390, 1298, 1508, 2029
Bsh1236I CGCG 1 cut(s) 2427
Bsh1285I CGRYCG 1 cut(s) 1954
BshTI ACCGGT 1 cut(s) 2183
BshVI ATCGAT 2 cut(s) 117, 1950
BsiEI CGRYCG 1 cut(s) 1954
BsiHKAI GWGCWC 2 cut(s) 72, 2296
BsiHKCI CYCGRG 1 cut(s) 2311
BsiSI CCGG 8 cut(s) 417, 566, 642, 1364, 1694, 1825, 1890, 2184
BslFI GGGAC 5 cut(s) 513, 632, 1950, 2016, 2035
BslI CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2275, 2550
BsmAI GTCTC 1 cut(s) 1795
BsmFI GGGAC 5 cut(s) 513, 632, 1950, 2016, 2035
BsoBI CYCGRG 1 cut(s) 2311
Bsp1286I GDGCHC 3 cut(s) 72, 336, 2296
Bsp1720I GCTNAGC 1 cut(s) 1625
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 6 cut(s) 626, 774, 1248, 1461, 2142, 2425
BspCNI CTCAG 2 cut(s) 58, 1638
BspDI ATCGAT 2 cut(s) 117, 1950
BspFNI CGCG 1 cut(s) 2427
BspLI GGNNCC 7 cut(s) 148, 335, 529, 925, 1081, 1804, 1823
BspMAI CTGCAG 2 cut(s) 2003, 2289
BspPI GGATC 8 cut(s) 141, 154, 1279, 1816, 1829, 1955, 2141, 2465
BspTI CTTAAG 1 cut(s) 1766
BsrBI CCGCTC 1 cut(s) 774
BsrDI GCAATG 2 cut(s) 465, 2251
BsrFI RCCGGY 2 cut(s) 1363, 2183
BsrI ACTGG 5 cut(s) 614, 939, 1586, 1979, 2350
BssAI RCCGGY 2 cut(s) 1363, 2183
BssECI CCNNGG 8 cut(s) 650, 1199, 1456, 1687, 1717, 1825, 2297, 2400
BssT1I CCWWGG 3 cut(s) 650, 1199, 1687
Bst2UI CCWGG 2 cut(s) 267, 2298
Bst4CI ACNGT 8 cut(s) 88, 319, 707, 716, 1066, 1298, 1486, 1530
Bst6I CTCTTC 2 cut(s) 1057, 1342
BstAFI CTTAAG 1 cut(s) 1766
BstAPI GCANNNNNTGC 1 cut(s) 2007
BstBAI YACGTR 1 cut(s) 1276
BstC8I GCNNGC 3 cut(s) 592, 700, 750
BstDEI CTNAG 3 cut(s) 28, 66, 1625
BstDSI CCRYGG 3 cut(s) 650, 1717, 2400
BstF5I GGATG 6 cut(s) 72, 226, 534, 1212, 1711, 1983
BstFNI CGCG 1 cut(s) 2427
BstHHI GCGC 2 cut(s) 351, 1127
BstMAI GTCTC 1 cut(s) 1795
BstMCI CGRYCG 1 cut(s) 1954
BstMWI GCNNNNNNNGC 7 cut(s) 355, 584, 623, 1502, 1844, 2007, 2433
BstNI CCWGG 2 cut(s) 267, 2298
BstNSI RCATGY 2 cut(s) 702, 886
BstSCI CCNGG 5 cut(s) 265, 415, 1824, 1888, 2296
BstSFI CTRYAG 3 cut(s) 1062, 1999, 2285
BstSLI GKGCMC 1 cut(s) 2296
BstUI CGCG 1 cut(s) 2427
BstV1I GCAGC 3 cut(s) 574, 601, 1139
BstV2I GAAGAC 2 cut(s) 675, 1507
BstX2I RGATCY 4 cut(s) 146, 1821, 2146, 2470
BstYI RGATCY 4 cut(s) 146, 1821, 2146, 2470
Bsu15I ATCGAT 2 cut(s) 117, 1950
BsuI GTATCC 1 cut(s) 631
BsuTUI ATCGAT 2 cut(s) 117, 1950
BtgI CCRYGG 3 cut(s) 650, 1717, 2400
BtsCI GGATG 6 cut(s) 72, 226, 534, 1212, 1711, 1983
BtsI GCAGTG 3 cut(s) 76, 1619, 2294
BtsIMutI CAGTG 4 cut(s) 76, 1619, 2184, 2294
Cac8I GCNNGC 3 cut(s) 592, 700, 750
CfoI GCGC 2 cut(s) 351, 1127
Cfr10I RCCGGY 2 cut(s) 1363, 2183
Cfr13I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
ClaI ATCGAT 2 cut(s) 117, 1950
CseI GACGC 1 cut(s) 2116
Csp6I GTAC 4 cut(s) 413, 1423, 1867, 2486
CspAI ACCGGT 1 cut(s) 2183
CviQI GTAC 4 cut(s) 413, 1423, 1867, 2486
DdeI CTNAG 3 cut(s) 28, 66, 1625
DraIII CACNNNGTG 2 cut(s) 524, 2401
DriI GACNNNNNGTC 3 cut(s) 1296, 1735, 2028
EaeI YGGCCR 4 cut(s) 121, 592, 1714, 2056
Eam1104I CTCTTC 2 cut(s) 1057, 1342
Eam1105I GACNNNNNGTC 3 cut(s) 1296, 1735, 2028
EarI CTCTTC 2 cut(s) 1057, 1342
EciI GGCGGA 2 cut(s) 1476, 2157
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 3 cut(s) 650, 1199, 1687
Eco147I AGGCCT 1 cut(s) 189
Eco24I GRGCYC 2 cut(s) 72, 336
Eco32I GATATC 1 cut(s) 1333
Eco47I GGWCC 4 cut(s) 527, 563, 1112, 1691
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 2 cut(s) 1025, 1038
Eco72I CACGTG 1 cut(s) 1276
Eco88I CYCGRG 1 cut(s) 2311
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 923
EcoRII CCWGG 2 cut(s) 265, 2296
EcoRV GATATC 1 cut(s) 1333
EcoT14I CCWWGG 3 cut(s) 650, 1199, 1687
EcoT22I ATGCAT 1 cut(s) 700
EcoT38I GRGCYC 2 cut(s) 72, 336
ErhI CCWWGG 3 cut(s) 650, 1199, 1687
FalI AAGNNNNNCTT 4 cut(s) 1153, 1185, 1715, 1747
FaqI GGGAC 5 cut(s) 513, 632, 1950, 2016, 2035
FauNDI CATATG 1 cut(s) 868
FbaI TGATCA 1 cut(s) 978
Fnu4HI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
FokI GGATG 6 cut(s) 59, 233, 521, 1199, 1718, 1990
FriOI GRGCYC 2 cut(s) 72, 336
Fsp4HI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
FspBI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
FspI TGCGCA 2 cut(s) 350, 1126
GlaI GCGC 2 cut(s) 350, 1126
GluI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
GsuI CTGGAG 1 cut(s) 939
HapII CCGG 8 cut(s) 417, 566, 642, 1364, 1694, 1825, 1890, 2184
HgaI GACGC 1 cut(s) 2116
HhaI GCGC 2 cut(s) 351, 1127
Hin6I GCGC 2 cut(s) 349, 1125
HinP1I GCGC 2 cut(s) 349, 1125
HincII GTYRAC 4 cut(s) 1438, 1522, 1591, 2045
HindII GTYRAC 4 cut(s) 1438, 1522, 1591, 2045
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 5 cut(s) 645, 723, 846, 1667, 1683
HpaII CCGG 8 cut(s) 417, 566, 642, 1364, 1694, 1825, 1890, 2184
HphI GGTGA 6 cut(s) 82, 463, 797, 2006, 2207, 2258
Hpy166II GTNNAC 9 cut(s) 471, 759, 783, 1037, 1438, 1522, 1591, 2045, 2294
Hpy188I TCNGA 8 cut(s) 67, 908, 983, 1005, 1057, 1453, 2336, 2481
Hpy8I GTNNAC 9 cut(s) 471, 759, 783, 1037, 1438, 1522, 1591, 2045, 2294
Hpy99I CGWCG 4 cut(s) 1294, 1741, 2110, 2524
HpyAV CCTTC 8 cut(s) 97, 179, 270, 347, 850, 937, 1000, 1226
HpyCH4III ACNGT 8 cut(s) 88, 319, 707, 716, 1066, 1298, 1486, 1530
HpyCH4IV ACGT 5 cut(s) 237, 1268, 1275, 1736, 2375
HpyF10VI GCNNNNNNNGC 7 cut(s) 355, 584, 623, 1502, 1844, 2007, 2433
HpyF3I CTNAG 3 cut(s) 28, 66, 1625
HpySE526I ACGT 5 cut(s) 237, 1268, 1275, 1736, 2375
HspAI GCGC 2 cut(s) 349, 1125
Ksp22I TGATCA 1 cut(s) 978
LmnI GCTCC 9 cut(s) 339, 584, 771, 961, 1009, 1079, 1157, 1558, 1802
Lsp1109I GCAGC 3 cut(s) 574, 601, 1139
LweI GCATC 7 cut(s) 429, 524, 664, 943, 1112, 1221, 1971
MaeI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
MaeII ACGT 5 cut(s) 237, 1268, 1275, 1736, 2375
MbiI CCGCTC 1 cut(s) 774
MflI RGATCY 4 cut(s) 146, 1821, 2146, 2470
MhlI GDGCHC 3 cut(s) 72, 336, 2296
MlsI TGGCCA 4 cut(s) 123, 594, 1716, 2058
MluNI TGGCCA 4 cut(s) 123, 594, 1716, 2058
MlyI GAGTC 2 cut(s) 654, 1676
MmeI TCCRAC 4 cut(s) 610, 1351, 1618, 1893
Mox20I TGGCCA 4 cut(s) 123, 594, 1716, 2058
Mph1103I ATGCAT 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 1748
MscI TGGCCA 4 cut(s) 123, 594, 1716, 2058
MseI TTAA 6 cut(s) 450, 606, 1733, 1767, 2099, 2319
MslI CAYNNNNRTG 2 cut(s) 44, 2217
Msp20I TGGCCA 4 cut(s) 123, 594, 1716, 2058
MspA1I CMGCKG 2 cut(s) 917, 1496
MspCI CTTAAG 1 cut(s) 1766
MspI CCGG 8 cut(s) 417, 566, 642, 1364, 1694, 1825, 1890, 2184
MspR9I CCNGG 5 cut(s) 267, 417, 1826, 1890, 2298
MvaI CCWGG 2 cut(s) 267, 2298
MvnI CGCG 1 cut(s) 2427
MwoI GCNNNNNNNGC 7 cut(s) 355, 584, 623, 1502, 1844, 2007, 2433
NciI CCSGG 3 cut(s) 417, 1826, 1890
NcoI CCATGG 1 cut(s) 650
NdeI CATATG 1 cut(s) 868
NlaIV GGNNCC 7 cut(s) 148, 335, 529, 925, 1081, 1804, 1823
NmeAIII GCCGAG 1 cut(s) 1437
NmuCI GTSAC 9 cut(s) 88, 1066, 1165, 1269, 1409, 1830, 1994, 2179, 2195
NsbI TGCGCA 2 cut(s) 350, 1126
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 2 cut(s) 702, 886
PaeI GCATGC 1 cut(s) 702
PaeR7I CTCGAG 1 cut(s) 2311
PceI AGGCCT 1 cut(s) 189
PciI ACATGT 1 cut(s) 882
PcsI WCGNNNNNNNCGW 3 cut(s) 234, 1298, 2381
PdmI GAANNNNTTC 1 cut(s) 1748
PfeI GAWTC 3 cut(s) 723, 846, 1683
PflMI CCANNNNNTGG 1 cut(s) 1652
PinAI ACCGGT 1 cut(s) 2183
PkrI GCNGC 5 cut(s) 589, 616, 776, 1129, 2426
Ple19I CGATCG 1 cut(s) 1954
PleI GAGTC 2 cut(s) 653, 1675
PmaCI CACGTG 1 cut(s) 1276
PmlI CACGTG 1 cut(s) 1276
PpsI GAGTC 2 cut(s) 653, 1675
Ppu21I YACGTR 1 cut(s) 1276
PscI ACATGT 1 cut(s) 882
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 265, 2296
PspCI CACGTG 1 cut(s) 1276
PspGI CCWGG 2 cut(s) 265, 2296
PspN4I GGNNCC 7 cut(s) 148, 335, 529, 925, 1081, 1804, 1823
PspPI GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
PsrI GAACNNNNNNTAC 2 cut(s) 1029, 1061
PstI CTGCAG 2 cut(s) 2003, 2289
PsuI RGATCY 4 cut(s) 146, 1821, 2146, 2470
PvuI CGATCG 1 cut(s) 1954
PvuII CAGCTG 2 cut(s) 917, 1496
RsaI GTAC 4 cut(s) 414, 1424, 1868, 2487
RsaNI GTAC 4 cut(s) 413, 1423, 1867, 2486
RseI CAYNNNNRTG 2 cut(s) 44, 2217
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 6 cut(s) 450, 606, 1733, 1767, 2099, 2319
SatI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
Sau96I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
SchI GAGTC 2 cut(s) 654, 1676
ScrFI CCNGG 5 cut(s) 267, 417, 1826, 1890, 2298
SduI GDGCHC 3 cut(s) 72, 336, 2296
SfaNI GCATC 7 cut(s) 429, 524, 664, 943, 1112, 1221, 1971
SfcI CTRYAG 3 cut(s) 1062, 1999, 2285
Sfr274I CTCGAG 1 cut(s) 2311
SinI GGWCC 4 cut(s) 527, 563, 1112, 1691
SlaI CTCGAG 1 cut(s) 2311
SmiMI CAYNNNNRTG 2 cut(s) 44, 2217
SmlI CTYRAG 2 cut(s) 1766, 2311
SmoI CTYRAG 2 cut(s) 1766, 2311
SpeI ACTAGT 1 cut(s) 1406
SphI GCATGC 1 cut(s) 702
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 6 cut(s) 626, 774, 1248, 1461, 2142, 2425
SspMI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
SstI GAGCTC 1 cut(s) 72
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 5 cut(s) 265, 415, 1824, 1888, 2296
StyI CCWWGG 3 cut(s) 650, 1199, 1687
TaaI ACNGT 8 cut(s) 88, 319, 707, 716, 1066, 1298, 1486, 1530
TaiI ACGT 5 cut(s) 240, 1271, 1278, 1739, 2378
TatI WGTACW 1 cut(s) 2485
TauI GCSGC 2 cut(s) 777, 2427
TfiI GAWTC 3 cut(s) 723, 846, 1683
Tru1I TTAA 6 cut(s) 450, 606, 1733, 1767, 2099, 2319
Tru9I TTAA 6 cut(s) 450, 606, 1733, 1767, 2099, 2319
TscAI CASTG 4 cut(s) 83, 1626, 2184, 2294
TseFI GTSAC 9 cut(s) 88, 1066, 1165, 1269, 1409, 1830, 1994, 2179, 2195
TseI GCWGC 3 cut(s) 587, 614, 1127
Tsp45I GTSAC 9 cut(s) 88, 1066, 1165, 1269, 1409, 1830, 1994, 2179, 2195
TspRI CASTG 4 cut(s) 83, 1626, 2184, 2294
Van91I CCANNNNNTGG 1 cut(s) 1652
Vha464I CTTAAG 1 cut(s) 1766
VneI GTGCAC 1 cut(s) 2292
VpaK11BI GGWCC 4 cut(s) 527, 563, 1112, 1691
XapI RAATTY 6 cut(s) 379, 788, 1783, 2113, 2227, 2251
XbaI TCTAGA 1 cut(s) 1378
XceI RCATGY 2 cut(s) 702, 886
XcmI CCANNNNNNNNNTGG 1 cut(s) 2070
XhoI CTCGAG 1 cut(s) 2311
XmnI GAANNNNTTC 1 cut(s) 1748
XspI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.