pycom15g02940

Exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006651
Physical Location & Seq
Forward (+)
1819548 .. 1828056
8509 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2649 bp
ATGGCGAAATCGAGCGCAGACGACCAGGAGCTGCGGCGGGCCTGCGAGGCCGCCATCGAAGGCACCAAGCAGAGCGTCGTTATGTCCATCCGCGTGGCCAAGAGCCGTGGGATCTGGGGCAAGACCCACAAGCTCGGCCGCGACATGGCCAAACCTAGGGTTCTCGCACTTTCCGTAAAAACCAAAGGCCAGAGGACTCAAGCCTTTCTTCGAGTCTTAAAATATTCTACTGGAGGAGTCCTTGAGCCTGCAAAGTTATACAAACTAAAGCATCTTTCAAAGGTGGAGGTTTTAACAAACGACCCTAGTGGGTGTACTTTTACTCTGGGTTTTGATAATCTTAGGAGTCAAAGTGTTGCTCCTCCTCAATGGACCATGCGAAATATTGATGACAGGAACCGGCTTTTACTTTGTATTTTAAACACATGCAAAGATGCACTGGGTCATCTTCCTAAAGTTGTTGGTATAGATGTCGTGGAGATGGCTCTTTGGGCTAAGGAAAATACACCGGCAGTCACTAGTCAAGGAAAGGAGCAGGAGGGACCTGCTGCATCAACAGTAACTGAAAGTGACTTGAAAGTAACTGTTGAAAAAGAACTTGTCTCCCAAGCTGAGGAAGAGGACATGGAGGCACTTTTGGGAACTTATGTCATGGGTATTGGTGAAGCAGAAGCATTTTCTGAAAGGTTGAAGAGAGAGCTTCTGGCTTTGGAAGCAGCAAATGTGCATGCCATTTTGGAAAGTGAACCTTTGATAGACGAGGTATTGCAAGGGCTTGAAGCAGCAACAAATTGTGTCGATGACATGGATGAATGGTTAGGAATCTTTAATGTGAAACTCAGACACATGAGAGAAGACATTGAATCGATAGAAACCCGCAATAACAAGTTGGAGATGCAATCTGTAAACAATAGGGCACTCATTTATGAACTCGATAAGCTTCTTCTAGGTTTGCGTGTTCCTTCTCAGTATGCAGCATGCTTGACAGGAGGTTCATTCGATGAAGCACGTATGCTTCAAAACATAGAAGCATGTGAGTGGTTGGCTGGTGCTTTACGCAGTCTTGAAGTACCTAACTTGGATCCCATCTATGCAAATATGCGGGCTGTTAAAGAGAAGCGAGCAGAACTTGAAAAATTGAAATCTACATTTGTCAGAAGAGCTTCTGAGTTCTTGCGAAATTACTTTGCTAGTTTGGTGGATTTTATGATAAGCGACAAGAGCTACTTTTCTCAGCGGGGGCAGCTGAAAAGGCCTGATCATGCTGATCTACGGTACAAATGCAGAACGTATGCTCGCCTTCTGCAACATCTGAAGAGTCTTGATAAAAATTGCATGGGGGCTTTGAGGAAAGCATATTGTAGCTCCCTTAACTTGCTTCTTCGTCGCGAGGCTCGTGAATTTGCAAATGAGCTTCGTGCTAGTACAAAGGCATCAAGAAATCCAACCGTCTGGCTTGAAGCTTCTACTGGGTCTGGTCAAAATGTGAATGCTGCGGACACTTCTACTGTTTCTGAAGCTTATTCCAAGATGCTTACAATTTTTATCCCACTCCTTGTAGATGAGAGTTCTTTCTTTGCGCACTTTATGTGCTTTGAAGTTCCGGCACTTGTTCCTCCAGGGGGTACTGCTAATGGAGATAAATATGATGACACCAATGACGATGATTTGGGAATCATGGACATTGATGACAATGACAGCAAAGCTGGCAAAAGTTCAGGAGAGCTTGCAGCATTAAATGAATCTCTTCAGGACTTACTTGATGGAATCCAGGAAGACTTCTATGCCGTTGTGGACTGGGCATACAAGATCGATCCCTTACGCTGCATATCAATGCATGGGATTACAGAGCGCTATCTTTCTGGTCAAAAAGCTGATGCAGCAGGTTTTGTACGTCTCTTGCTTGGTGATCTGGAGTCAAGAGTTTCTGTGCAGTTCAGTCACTTTGTTGATGAAGCTTGCCGCCAGATTGAAAGAAACGAGCGCAACGTTAGACAAATGGGTGTCTTATCATACATCCCAAGATTTGCTACTCTGGCAACACGAATGGAGCAGTACATCCAGGGACAATCTAGGGATTTGGTTGATCAAGCGTACACAAAATTTGTTAGTATAATGTTTGCGACATTGGAGAAAATTGCACAAACAGAGCCAAAGTATTCTGATCTTTTTCTATTAGAGAACTACGCTGCGTTTCAGAATAGTTTGTATGACCTAGCAAATGTTGTGCCCACCTTGGCCAAATTTTATCACCAGGCAAGTGAAGCTTATGAACAAGCTTGCACACGCCATATCAGCATGATCATATACAATCAATTTGAACGGCTTTTCCAGTTTGCTCGAAGAATAGAGGATTTGATGTATACAATCGCACCAGAAGAGATCCCCTTCCAGCTAGGGTTGTCAAAGATGGATCTGCGGAAGGTGATAAAATCAAGTTTATCCGGGCTTGACAAGTCCATCACTGCAATGTACAAGAAGTTGCAGAAAAACATGACGTCAGAAGAACTGCTGCCTTCATTATGGGATAAATGCAAGAAGGAGTTCCTTGACAAGTATGAAAGTTTTGCGCAACTCGTCGCCAAGATTTACCCGACAGAGACCATTCATAGCGTAACAGAAATGAGAGACCTTTTGGCTTCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec3-PIP2_bind Sec3_C

Protein Analysis

883

Amino Acids

99.52

Weight (kDa)

5.67

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec3-PIP2_bind PF15277 50 - 144 8.7e-15 Exocyst complex component SEC3 N-terminal PIP2 binding PH
Sec3_CC PF09763 223 - 344 1.7e-24 Exocyst complex component Sec3, coiled-coil
Vps52_CC PF04129 255 - 392 1.2e-07 Vps52/Sac2 family, coiled-coil
Sec3_C-term PF20654 577 - 865 1.9e-28 Exocyst complex component Sec3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2501
Acc16I TGCGCA 2 cut(s) 1581, 2571
Acc36I ACCTGC 2 cut(s) 553, 1874
AccB1I GGYRCC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 2363
AccII CGCG 3 cut(s) 93, 141, 1389
AclI AACGTT 1 cut(s) 1989
AclWI GGATC 6 cut(s) 119, 1076, 1089, 1808, 2377, 2421
AcoI YGGCCR 4 cut(s) 96, 136, 147, 2238
AcsI RAATTY 3 cut(s) 1400, 2102, 2243
AcuI CTGAAG 3 cut(s) 1334, 1536, 1733
AcyI GRCGYC 1 cut(s) 2498
AfaI GTAC 9 cut(s) 316, 1071, 1277, 1426, 1627, 1893, 2057, 2096, 2474
AfeI AGCGCT 1 cut(s) 1853
AfiI CCNNNNNNNGG 4 cut(s) 145, 156, 613, 1622
AhlI ACTAGT 1 cut(s) 518
AjnI CCWGG 5 cut(s) 24, 1618, 1770, 2061, 2253
AloI GAACNNNNNNTCC 2 cut(s) 2313, 2345
Alw26I GTCTC 4 cut(s) 607, 1901, 2594, 2622
AlwI GGATC 6 cut(s) 119, 1076, 1089, 1808, 2377, 2421
AlwNI CAGNNNCTG 2 cut(s) 31, 563
Aor51HI AGCGCT 1 cut(s) 1853
AoxI GGCC 8 cut(s) 39, 48, 96, 136, 147, 187, 1253, 2238
ApoI RAATTY 3 cut(s) 1400, 2102, 2243
Asp700I GAANNNNTTC 3 cut(s) 1162, 1746, 2543
AspA2I CCTAGG 1 cut(s) 155
AspLEI GCGC 5 cut(s) 17, 1582, 1854, 1986, 2572
AspS9I GGNCC 3 cut(s) 39, 372, 542
AsuC2I CCSGG 1 cut(s) 2446
AsuHPI GGTGA 4 cut(s) 674, 1919, 2243, 2437
AvaII GGWCC 2 cut(s) 372, 542
AvrII CCTAGG 1 cut(s) 155
BaeGI GKGCMC 2 cut(s) 919, 2232
BalI TGGCCA 3 cut(s) 98, 149, 2240
BamHI GGATCC 1 cut(s) 1081
BanI GGYRCC 1 cut(s) 62
BauI CACGAG 1 cut(s) 1395
BbsI GAAGAC 2 cut(s) 861, 1782
BbvCI CCTCAGC 1 cut(s) 612
BccI CCATC 7 cut(s) 62, 95, 475, 1094, 1757, 2404, 2468
BceAI ACGGC 3 cut(s) 90, 1772, 2339
BciT130I CCWGG 5 cut(s) 26, 1620, 1772, 2063, 2255
BclI TGATCA 3 cut(s) 1258, 2086, 2301
BcnI CCSGG 1 cut(s) 2446
BcoDI GTCTC 4 cut(s) 607, 1901, 2594, 2622
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 9 cut(s) 156, 306, 519, 947, 1191, 1422, 2073, 2216, 2396
BfoI RGCGCY 1 cut(s) 1855
BfuAI ACCTGC 2 cut(s) 553, 1874
BglI GCCNNNNNGGC 1 cut(s) 47
BlnI CCTAGG 1 cut(s) 155
Bme1390I CCNGG 6 cut(s) 26, 1620, 1772, 2063, 2255, 2446
Bme18I GGWCC 2 cut(s) 372, 542
BmgT120I GGNCC 3 cut(s) 39, 372, 542
BmiI GGNNCC 4 cut(s) 64, 398, 543, 1083
BmrFI CCNGG 6 cut(s) 26, 1620, 1772, 2063, 2255, 2446
BmrI ACTGGG 3 cut(s) 449, 1479, 1807
BmsI GCATC 7 cut(s) 280, 424, 560, 885, 1442, 1521, 1867
BmuI ACTGGG 3 cut(s) 449, 1479, 1807
BpiI GAAGAC 2 cut(s) 861, 1782
BpmI CTGGAG 3 cut(s) 252, 1602, 1934
Bpu10I CCTNAGC 2 cut(s) 495, 612
BpuEI CTTGAG 2 cut(s) 183, 263
BpuMI CCSGG 1 cut(s) 2446
Bsa29I ATCGAT 2 cut(s) 866, 1812
BsaAI YACGTR 1 cut(s) 1010
BsaHI GRCGYC 1 cut(s) 2498
BsaI GGTCTC 2 cut(s) 2594, 2622
BsaJI CCNNGG 5 cut(s) 106, 155, 1619, 2062, 2235
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 156, 613, 1622
Bse118I RCCGGY 2 cut(s) 399, 508
Bse1I ACTGG 5 cut(s) 235, 444, 1474, 1802, 2332
Bse3DI GCAATG 1 cut(s) 2475
BseBI CCWGG 5 cut(s) 26, 1620, 1772, 2063, 2255
BseCI ATCGAT 2 cut(s) 866, 1812
BseDI CCNNGG 5 cut(s) 106, 155, 1619, 2062, 2235
BseGI GGATG 4 cut(s) 87, 814, 2016, 2058
BseLI CCNNNNNNNGG 4 cut(s) 145, 156, 613, 1622
BseMI GCAATG 1 cut(s) 2475
BseMII CTCAG 5 cut(s) 603, 853, 980, 1158, 1247
BseNI ACTGG 5 cut(s) 235, 444, 1474, 1802, 2332
BseRI GAGGAG 3 cut(s) 249, 351, 354
BseSI GKGCMC 2 cut(s) 919, 2232
BseX3I CGGCCG 1 cut(s) 136
BsgI GTGCAG 1 cut(s) 1952
Bsh1236I CGCG 3 cut(s) 93, 141, 1389
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 8 cut(s) 41, 50, 98, 138, 149, 189, 1255, 2240
BshNI GGYRCC 1 cut(s) 62
BshVI ATCGAT 2 cut(s) 866, 1812
BsiEI CGRYCG 1 cut(s) 139
BsiSI CCGG 4 cut(s) 400, 509, 1604, 2445
BslFI GGGAC 2 cut(s) 555, 2079
BslI CCNNNNNNNGG 4 cut(s) 145, 156, 613, 1622
BsmAI GTCTC 4 cut(s) 607, 1901, 2594, 2622
BsmBI CGTCTC 1 cut(s) 1901
BsmFI GGGAC 2 cut(s) 555, 2079
BsmI GAATGC 1 cut(s) 1495
BsnI GGCC 8 cut(s) 41, 50, 98, 138, 149, 189, 1255, 2240
Bso31I GGTCTC 2 cut(s) 2594, 2622
Bsp1286I GDGCHC 2 cut(s) 919, 2232
Bsp1407I TGTACA 1 cut(s) 2472
Bsp68I TCGCGA 1 cut(s) 1389
BspANI GGCC 8 cut(s) 41, 50, 98, 138, 149, 189, 1255, 2240
BspCNI CTCAG 5 cut(s) 604, 852, 979, 1159, 1246
BspDI ATCGAT 2 cut(s) 866, 1812
BspFNI CGCG 3 cut(s) 93, 141, 1389
BspLI GGNNCC 4 cut(s) 64, 398, 543, 1083
BspMI ACCTGC 2 cut(s) 553, 1874
BspPI GGATC 6 cut(s) 119, 1076, 1089, 1808, 2377, 2421
BspQI GCTCTTC 1 cut(s) 1153
BspT107I GGYRCC 1 cut(s) 62
BspTNI GGTCTC 2 cut(s) 2594, 2622
BsrDI GCAATG 1 cut(s) 2475
BsrFI RCCGGY 2 cut(s) 399, 508
BsrGI TGTACA 1 cut(s) 2472
BsrI ACTGG 5 cut(s) 235, 444, 1474, 1802, 2332
BssAI RCCGGY 2 cut(s) 399, 508
BssECI CCNNGG 5 cut(s) 106, 155, 1619, 2062, 2235
BssNAI GTATAC 1 cut(s) 2364
BssNI GRCGYC 1 cut(s) 2498
BssSI CACGAG 1 cut(s) 1395
BssT1I CCWWGG 2 cut(s) 155, 2235
Bst1107I GTATAC 1 cut(s) 2364
Bst2BI CACGAG 1 cut(s) 1395
Bst2UI CCWGG 5 cut(s) 26, 1620, 1772, 2063, 2255
Bst4CI ACNGT 5 cut(s) 559, 586, 1275, 1450, 1510
Bst6I CTCTTC 6 cut(s) 612, 686, 1153, 1310, 1752, 2373
BstACI GRCGYC 1 cut(s) 2498
BstAUI TGTACA 1 cut(s) 2472
BstBAI YACGTR 1 cut(s) 1010
BstDEI CTNAG 7 cut(s) 341, 495, 612, 839, 966, 1167, 1233
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 4 cut(s) 87, 814, 2016, 2058
BstFNI CGCG 3 cut(s) 93, 141, 1389
BstH2I RGCGCY 1 cut(s) 1855
BstHHI GCGC 5 cut(s) 17, 1582, 1854, 1986, 2572
BstMAI GTCTC 4 cut(s) 607, 1901, 2594, 2622
BstMCI CGRYCG 1 cut(s) 139
BstNI CCWGG 5 cut(s) 26, 1620, 1772, 2063, 2255
BstNSI RCATGY 4 cut(s) 429, 731, 981, 1035
BstSCI CCNGG 6 cut(s) 24, 1618, 1770, 2061, 2253, 2444
BstSLI GKGCMC 2 cut(s) 919, 2232
BstUI CGCG 3 cut(s) 93, 141, 1389
BstV2I GAAGAC 2 cut(s) 861, 1782
BstX2I RGATCY 4 cut(s) 111, 1081, 2382, 2413
BstXI CCANNNNNNTGG 2 cut(s) 94, 1452
BstYI RGATCY 4 cut(s) 111, 1081, 2382, 2413
BstZ17I GTATAC 1 cut(s) 2364
BstZI CGGCCG 1 cut(s) 136
Bsu15I ATCGAT 2 cut(s) 866, 1812
BsuRI GGCC 8 cut(s) 41, 50, 98, 138, 149, 189, 1255, 2240
BsuTUI ATCGAT 2 cut(s) 866, 1812
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 4 cut(s) 87, 814, 2016, 2058
BtsI GCAGTG 1 cut(s) 2463
BtsIMutI CAGTG 2 cut(s) 437, 2463
BtuMI TCGCGA 1 cut(s) 1389
BveI ACCTGC 2 cut(s) 553, 1874
CaiI CAGNNNCTG 2 cut(s) 31, 563
CfoI GCGC 5 cut(s) 17, 1582, 1854, 1986, 2572
Cfr10I RCCGGY 2 cut(s) 399, 508
Cfr13I GGNCC 3 cut(s) 39, 372, 542
ClaI ATCGAT 2 cut(s) 866, 1812
CseI GACGC 1 cut(s) 64
Csp6I GTAC 9 cut(s) 315, 1070, 1276, 1425, 1626, 1892, 2056, 2095, 2473
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviQI GTAC 9 cut(s) 315, 1070, 1276, 1425, 1626, 1892, 2056, 2095, 2473
DdeI CTNAG 7 cut(s) 341, 495, 612, 839, 966, 1167, 1233
DraI TTTAAA 1 cut(s) 420
EaeI YGGCCR 4 cut(s) 96, 136, 147, 2238
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 6 cut(s) 612, 686, 1153, 1310, 1752, 2373
EarI CTCTTC 6 cut(s) 612, 686, 1153, 1310, 1752, 2373
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 2 cut(s) 155, 2235
Eco147I AGGCCT 1 cut(s) 1255
Eco31I GGTCTC 2 cut(s) 2594, 2622
Eco47I GGWCC 2 cut(s) 372, 542
Eco47III AGCGCT 1 cut(s) 1853
Eco52I CGGCCG 1 cut(s) 136
Eco57I CTGAAG 3 cut(s) 1334, 1536, 1733
EcoO109I RGGNCCY 1 cut(s) 542
EcoRII CCWGG 5 cut(s) 24, 1618, 1770, 2061, 2253
EcoT14I CCWWGG 2 cut(s) 155, 2235
EcoT22I ATGCAT 1 cut(s) 1839
ErhI CCWWGG 2 cut(s) 155, 2235
Esp3I CGTCTC 1 cut(s) 1901
FalI AAGNNNNNCTT 8 cut(s) 192, 224, 733, 765, 1211, 1243, 2251, 2283
FaqI GGGAC 2 cut(s) 555, 2079
FauI CCCGC 4 cut(s) 30, 884, 1095, 1230
FbaI TGATCA 3 cut(s) 1258, 2086, 2301
FblI GTMKAC 1 cut(s) 2363
FokI GGATG 4 cut(s) 74, 821, 2003, 2045
FspBI CTAG 9 cut(s) 156, 306, 519, 947, 1191, 1422, 2073, 2216, 2396
FspI TGCGCA 2 cut(s) 1581, 2571
GlaI GCGC 5 cut(s) 16, 1581, 1853, 1985, 2571
GsuI CTGGAG 3 cut(s) 252, 1602, 1934
HaeII RGCGCY 1 cut(s) 1855
HaeIII GGCC 8 cut(s) 41, 50, 98, 138, 149, 189, 1255, 2240
HapII CCGG 4 cut(s) 400, 509, 1604, 2445
HgaI GACGC 1 cut(s) 64
HhaI GCGC 5 cut(s) 17, 1582, 1854, 1986, 2572
Hin1I GRCGYC 1 cut(s) 2498
Hin6I GCGC 5 cut(s) 15, 1580, 1852, 1984, 2570
HinP1I GCGC 5 cut(s) 15, 1580, 1852, 1984, 2570
HindIII AAGCTT 6 cut(s) 938, 1461, 1518, 1956, 2265, 2277
HpaII CCGG 4 cut(s) 400, 509, 1604, 2445
HphI GGTGA 4 cut(s) 674, 1919, 2243, 2437
Hpy166II GTNNAC 6 cut(s) 315, 746, 907, 1795, 2097, 2364
Hpy188I TCNGA 9 cut(s) 682, 842, 1157, 1168, 1314, 1516, 2164, 2199, 2503
Hpy188III TCNNGA 9 cut(s) 1064, 1322, 1388, 1397, 1437, 1719, 1751, 1913, 1920
Hpy8I GTNNAC 6 cut(s) 315, 746, 907, 1795, 2097, 2364
Hpy99I CGWCG 3 cut(s) 80, 1389, 2582
HpyAV CCTTC 7 cut(s) 53, 972, 1310, 2398, 2416, 2526, 2533
HpyCH4III ACNGT 5 cut(s) 559, 586, 1275, 1450, 1510
HpyCH4IV ACGT 5 cut(s) 1009, 1289, 1894, 1989, 2498
HpyF3I CTNAG 7 cut(s) 341, 495, 612, 839, 966, 1167, 1233
HpySE526I ACGT 5 cut(s) 1009, 1289, 1894, 1989, 2498
Hsp92I GRCGYC 1 cut(s) 2498
HspAI GCGC 5 cut(s) 15, 1580, 1852, 1984, 2570
Ksp22I TGATCA 3 cut(s) 1258, 2086, 2301
LguI GCTCTTC 1 cut(s) 1153
LmnI GCTCC 5 cut(s) 28, 364, 532, 1370, 2050
LweI GCATC 7 cut(s) 280, 424, 560, 885, 1442, 1521, 1867
MaeI CTAG 9 cut(s) 156, 306, 519, 947, 1191, 1422, 2073, 2216, 2396
MaeII ACGT 5 cut(s) 1009, 1289, 1894, 1989, 2498
MaeIII GTNAC 6 cut(s) 514, 559, 569, 580, 1940, 2614
MflI RGATCY 4 cut(s) 111, 1081, 2382, 2413
MhlI GDGCHC 2 cut(s) 919, 2232
MlsI TGGCCA 3 cut(s) 98, 149, 2240
MluNI TGGCCA 3 cut(s) 98, 149, 2240
MlyI GAGTC 6 cut(s) 190, 222, 246, 355, 1327, 1925
MmeI TCCRAC 2 cut(s) 870, 1469
Mox20I TGGCCA 3 cut(s) 98, 149, 2240
Mph1103I ATGCAT 1 cut(s) 1839
MroXI GAANNNNTTC 3 cut(s) 1162, 1746, 2543
MscI TGGCCA 3 cut(s) 98, 149, 2240
MseI TTAA 7 cut(s) 218, 293, 419, 828, 1110, 1371, 1736
MslI CAYNNNNRTG 4 cut(s) 92, 1036, 1832, 2468
Msp20I TGGCCA 3 cut(s) 98, 149, 2240
MspA1I CMGCKG 2 cut(s) 1237, 1246
MspI CCGG 4 cut(s) 400, 509, 1604, 2445
MspR9I CCNGG 6 cut(s) 26, 1620, 1772, 2063, 2255, 2446
Mva1269I GAATGC 1 cut(s) 1495
MvaI CCWGG 5 cut(s) 26, 1620, 1772, 2063, 2255
MvnI CGCG 3 cut(s) 93, 141, 1389
NciI CCSGG 1 cut(s) 2446
NlaIV GGNNCC 4 cut(s) 64, 398, 543, 1083
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 3 cut(s) 514, 569, 1940
NruI TCGCGA 1 cut(s) 1389
NsbI TGCGCA 2 cut(s) 1581, 2571
NsiI ATGCAT 1 cut(s) 1839
NspI RCATGY 4 cut(s) 429, 731, 981, 1035
PaeI GCATGC 2 cut(s) 731, 981
PceI AGGCCT 1 cut(s) 1255
PciSI GCTCTTC 1 cut(s) 1153
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 1393, 1986
PctI GAATGC 1 cut(s) 1495
PdmI GAANNNNTTC 3 cut(s) 1162, 1746, 2543
PfeI GAWTC 5 cut(s) 822, 863, 1674, 1742, 1767
PfoI TCCNGGA 1 cut(s) 1770
PleI GAGTC 6 cut(s) 190, 221, 245, 354, 1326, 1924
PpsI GAGTC 6 cut(s) 190, 221, 245, 354, 1326, 1924
Ppu21I YACGTR 1 cut(s) 1010
PpuMI RGGWCCY 1 cut(s) 542
Psp1406I AACGTT 1 cut(s) 1989
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 5 cut(s) 24, 1618, 1770, 2061, 2253
PspGI CCWGG 5 cut(s) 24, 1618, 1770, 2061, 2253
PspN4I GGNNCC 4 cut(s) 64, 398, 543, 1083
PspPI GGNCC 3 cut(s) 39, 372, 542
PspPPI RGGWCCY 1 cut(s) 542
PstNI CAGNNNCTG 2 cut(s) 31, 563
PsuI RGATCY 4 cut(s) 111, 1081, 2382, 2413
PvuII CAGCTG 1 cut(s) 1246
RruI TCGCGA 1 cut(s) 1389
RsaI GTAC 9 cut(s) 316, 1071, 1277, 1426, 1627, 1893, 2057, 2096, 2474
RsaNI GTAC 9 cut(s) 315, 1070, 1276, 1425, 1626, 1892, 2056, 2095, 2473
RseI CAYNNNNRTG 4 cut(s) 92, 1036, 1832, 2468
SapI GCTCTTC 1 cut(s) 1153
SaqAI TTAA 7 cut(s) 218, 293, 419, 828, 1110, 1371, 1736
Sau96I GGNCC 3 cut(s) 39, 372, 542
SchI GAGTC 6 cut(s) 190, 222, 246, 355, 1327, 1925
ScrFI CCNGG 6 cut(s) 26, 1620, 1772, 2063, 2255, 2446
SduI GDGCHC 2 cut(s) 919, 2232
SfaNI GCATC 7 cut(s) 280, 424, 560, 885, 1442, 1521, 1867
SfiI GGCCNNNNNGGCC 1 cut(s) 47
SinI GGWCC 2 cut(s) 372, 542
SmiMI CAYNNNNRTG 4 cut(s) 92, 1036, 1832, 2468
SmlI CTYRAG 2 cut(s) 198, 242
SmoI CTYRAG 2 cut(s) 198, 242
SpeI ACTAGT 1 cut(s) 518
SphI GCATGC 2 cut(s) 731, 981
SseBI AGGCCT 1 cut(s) 1255
SspI AATATT 2 cut(s) 224, 385
SspMI CTAG 9 cut(s) 156, 306, 519, 947, 1191, 1422, 2073, 2216, 2396
StuI AGGCCT 1 cut(s) 1255
StyD4I CCNGG 6 cut(s) 24, 1618, 1770, 2061, 2253, 2444
StyI CCWWGG 2 cut(s) 155, 2235
TaaI ACNGT 5 cut(s) 559, 586, 1275, 1450, 1510
TaiI ACGT 5 cut(s) 1012, 1292, 1897, 1992, 2501
TaqI TCGA 9 cut(s) 11, 57, 211, 798, 866, 933, 999, 1812, 2341
TatI WGTACW 4 cut(s) 314, 1424, 2055, 2472
TauI GCSGC 4 cut(s) 37, 53, 141, 1965
TfiI GAWTC 5 cut(s) 822, 863, 1674, 1742, 1767
Tru1I TTAA 7 cut(s) 218, 293, 419, 828, 1110, 1371, 1736
Tru9I TTAA 7 cut(s) 218, 293, 419, 828, 1110, 1371, 1736
TscAI CASTG 2 cut(s) 444, 2470
TseFI GTSAC 3 cut(s) 514, 569, 1940
Tsp45I GTSAC 3 cut(s) 514, 569, 1940
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 2 cut(s) 444, 2470
VpaK11BI GGWCC 2 cut(s) 372, 542
XapI RAATTY 3 cut(s) 1400, 2102, 2243
XceI RCATGY 4 cut(s) 429, 731, 981, 1035
XmaJI CCTAGG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 2363
XmnI GAANNNNTTC 3 cut(s) 1162, 1746, 2543
XspI CTAG 9 cut(s) 156, 306, 519, 947, 1191, 1422, 2073, 2216, 2396
ZraI GACGTC 1 cut(s) 2499
Zsp2I ATGCAT 1 cut(s) 1839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.