pycom15g03300

Protein OSB1, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009200
Physical Location & Seq
Reverse (-)
2054328 .. 2056032
1705 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 849 bp
ATGAAGGCGCTTCGTCGTCTACTCTCAATCAGCAAGCCAAGTCCCTCACTGTTGTTCTCCCAAAGGCTCTCCCTGTTCTCTTCAGCCACTAACCCTAGTTTTTCGAATTCCTTCTCATCCTCCGACGACGGCGACGAAGAAGAAGGCAGCGGCATTTACCGAAACAAACTCAAGTTCCAGCGTCCGAGAGCAATCACGCGGCGCCGTTGGGAGGAGAATTTGAATTTGGGGAACTCTGGTAGCTTTATCGGTACCGTGACTCACCCCCTTCGGGTCGTGAACAACAACGGCGGCCCCTTTGGAGTTCACACCGGCCTTCGCGTCAGAAACTCCCCGAAATCTGAGAGCAGTTTTTGGATATTGCTGAAGATGTGGGAGGAGATGGCAGAATTGTCCGTCAAACATTTGAAACCGAATGATTTTATTTATGTCTCTGGCCGTTTAGGTTCTTACACGAAGGTTGACCAGAATGGGAATTTGAGTATGCGTTATAAGTTAGACGTGAAGGAGTTGAATTACGTATCACAACATGGACACAGATTGAGCTCCAAAAATCACAAGGAACCACAATGCATTGAAGGTGGAGGTGATTTGGACAAGTATAAGGATCGCCTTCACTTGTGGCAAGTGTTTTTTAGCAATCCATATGAATGGTGGGATAACAGGGAAGGTAAGAAGAATCCGAGACAACCAGATTTCAAGCACAAGGATACGGGCGAAGCCCTCTGGCTGTCGCGAAATGATCCTCCGTGGATTGAAAAACAACTCCAGTTGCTTGATGAGAGAATGCCAAAACAAAGACACGGTGAACGACGTTCCTGTGTGTCAGAGTGGCAGTACGATGAATAG

Protein Analysis

283

Amino Acids

32.86

Weight (kDa)

9.46

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 100 - 172 8.4e-06 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
Acc65I GGTACC 1 cut(s) 251
AccB1I GGYRCC 2 cut(s) 201, 251
AccI GTMKAC 1 cut(s) 19
AccII CGCG 3 cut(s) 199, 321, 736
AciI CCGC 3 cut(s) 150, 199, 291
AclWI GGATC 2 cut(s) 615, 737
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 4 cut(s) 106, 217, 223, 475
AcuI CTGAAG 2 cut(s) 66, 386
AcyI GRCGYC 1 cut(s) 202
AfaI GTAC 2 cut(s) 253, 839
AfiI CCNNNNNNNGG 2 cut(s) 211, 271
AgsI TTSAA 6 cut(s) 223, 409, 514, 578, 700, 758
AjiI CACGTC 1 cut(s) 502
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 2 cut(s) 243, 546
AluI AGCT 2 cut(s) 243, 546
Alw21I GWGCWC 1 cut(s) 548
Alw26I GTCTC 2 cut(s) 436, 679
AlwI GGATC 2 cut(s) 615, 737
AoxI GGCC 3 cut(s) 292, 313, 436
ApeKI GCWGC 1 cut(s) 147
ApoI RAATTY 4 cut(s) 106, 217, 223, 475
Asp700I GAANNNNTTC 1 cut(s) 110
Asp718I GGTACC 1 cut(s) 251
AspLEI GCGC 2 cut(s) 10, 204
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 254, 599, 818
AsuII TTCGAA 1 cut(s) 104
BanI GGYRCC 2 cut(s) 201, 251
BanII GRGCYC 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 548
BbvI GCAGC 1 cut(s) 159
BccI CCATC 1 cut(s) 376
BceAI ACGGC 4 cut(s) 145, 189, 304, 423
BciVI GTATCC 1 cut(s) 703
BcoDI GTCTC 2 cut(s) 436, 679
BfaI CTAG 1 cut(s) 96
BfoI RGCGCY 2 cut(s) 11, 205
BfuI GTATCC 1 cut(s) 703
BisI GCNGC 4 cut(s) 148, 151, 200, 292
BlsI GCNGC 4 cut(s) 149, 152, 201, 293
BmgBI CACGTC 1 cut(s) 502
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 4 cut(s) 203, 253, 295, 564
BpmI CTGGAG 1 cut(s) 752
Bpu14I TTCGAA 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 520
BsaHI GRCGYC 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 749
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 271
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 1 cut(s) 769
BseDI CCNNGG 1 cut(s) 749
BseGI GGATG 1 cut(s) 116
BseLI CCNNNNNNNGG 2 cut(s) 211, 271
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 769
BseRI GAGGAG 2 cut(s) 227, 392
BseXI GCAGC 1 cut(s) 159
Bsh1236I CGCG 3 cut(s) 199, 321, 736
BshFI GGCC 3 cut(s) 294, 315, 438
BshNI GGYRCC 2 cut(s) 201, 251
BsiHKAI GWGCWC 1 cut(s) 548
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 27
BslI CCNNNNNNNGG 2 cut(s) 211, 271
BsmAI GTCTC 2 cut(s) 436, 679
BsmFI GGGAC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 792
BsnI GGCC 3 cut(s) 294, 315, 438
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 548
Bsp143I GATC 2 cut(s) 607, 742
Bsp68I TCGCGA 1 cut(s) 736
BspACI CCGC 3 cut(s) 150, 199, 291
BspANI GGCC 3 cut(s) 294, 315, 438
BspCNI CTCAG 1 cut(s) 334
BspFNI CGCG 3 cut(s) 199, 321, 736
BspLI GGNNCC 4 cut(s) 203, 253, 295, 564
BspPI GGATC 2 cut(s) 615, 737
BspT104I TTCGAA 1 cut(s) 104
BspT107I GGYRCC 2 cut(s) 201, 251
BsrFI RCCGGY 1 cut(s) 311
BsrI ACTGG 1 cut(s) 769
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 1 cut(s) 749
BssMI GATC 2 cut(s) 607, 742
BssNI GRCGYC 1 cut(s) 202
Bst4CI ACNGT 3 cut(s) 51, 256, 806
Bst6I CTCTTC 1 cut(s) 85
BstACI GRCGYC 1 cut(s) 202
BstBAI YACGTR 1 cut(s) 520
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 35
BstDEI CTNAG 1 cut(s) 342
BstDSI CCRYGG 1 cut(s) 749
BstF5I GGATG 1 cut(s) 116
BstFNI CGCG 3 cut(s) 199, 321, 736
BstH2I RGCGCY 2 cut(s) 11, 205
BstHHI GCGC 2 cut(s) 10, 204
BstKTI GATC 2 cut(s) 610, 745
BstMAI GTCTC 2 cut(s) 436, 679
BstMBI GATC 2 cut(s) 607, 742
BstSNI TACGTA 1 cut(s) 520
BstUI CGCG 3 cut(s) 199, 321, 736
BstV1I GCAGC 1 cut(s) 159
BstXI CCANNNNNNTGG 1 cut(s) 651
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 3 cut(s) 294, 315, 438
BtgI CCRYGG 1 cut(s) 749
BtrI CACGTC 1 cut(s) 502
BtsCI GGATG 1 cut(s) 116
BtsIMutI CAGTG 1 cut(s) 47
BtuMI TCGCGA 1 cut(s) 736
Cac8I GCNNGC 1 cut(s) 35
CfoI GCGC 2 cut(s) 10, 204
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 1 cut(s) 293
CseI GACGC 2 cut(s) 170, 310
Csp6I GTAC 2 cut(s) 252, 838
CviAII CATG 1 cut(s) 530
CviQI GTAC 2 cut(s) 252, 838
DdeI CTNAG 1 cut(s) 342
DinI GGCGCC 1 cut(s) 203
DpnI GATC 2 cut(s) 609, 744
DpnII GATC 2 cut(s) 607, 742
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 546
Eco105I TACGTA 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 548
Eco53kI GAGCTC 1 cut(s) 546
Eco57I CTGAAG 2 cut(s) 66, 386
EcoICRI GAGCTC 1 cut(s) 546
EcoRI GAATTC 1 cut(s) 106
EcoT22I ATGCAT 1 cut(s) 575
EcoT38I GRGCYC 1 cut(s) 548
EgeI GGCGCC 1 cut(s) 203
EheI GGCGCC 1 cut(s) 203
FaeI CATG 1 cut(s) 533
FaiI YATR 7 cut(s) 429, 485, 492, 531, 603, 646, 648
FaqI GGGAC 1 cut(s) 27
FatI CATG 1 cut(s) 529
FauNDI CATATG 1 cut(s) 646
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 4 cut(s) 148, 151, 200, 292
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 1 cut(s) 548
Fsp4HI GCNGC 4 cut(s) 148, 151, 200, 292
FspBI CTAG 1 cut(s) 96
GlaI GCGC 2 cut(s) 9, 203
GluI GCNGC 4 cut(s) 148, 151, 200, 292
GsuI CTGGAG 1 cut(s) 752
HaeII RGCGCY 2 cut(s) 11, 205
HaeIII GGCC 3 cut(s) 294, 315, 438
HapII CCGG 1 cut(s) 312
HgaI GACGC 2 cut(s) 170, 310
HhaI GCGC 2 cut(s) 10, 204
Hin1I GRCGYC 1 cut(s) 202
Hin1II CATG 1 cut(s) 533
Hin6I GCGC 2 cut(s) 8, 202
HinP1I GCGC 2 cut(s) 8, 202
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 2 cut(s) 259, 679
HpaII CCGG 1 cut(s) 312
HphI GGTGA 3 cut(s) 254, 599, 818
Hpy166II GTNNAC 5 cut(s) 20, 280, 307, 463, 809
Hpy188I TCNGA 6 cut(s) 124, 186, 326, 343, 684, 829
Hpy188III TCNNGA 2 cut(s) 277, 735
Hpy8I GTNNAC 5 cut(s) 20, 280, 307, 463, 809
Hpy99I CGWCG 5 cut(s) 18, 128, 131, 137, 816
HpyAV CCTTC 9 cut(s) 121, 137, 278, 326, 451, 499, 572, 623, 662
HpyCH4III ACNGT 3 cut(s) 51, 256, 806
HpyCH4IV ACGT 3 cut(s) 501, 519, 814
HpyCH4V TGCA 1 cut(s) 573
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 3 cut(s) 501, 519, 814
Hsp92I GRCGYC 1 cut(s) 202
Hsp92II CATG 1 cut(s) 533
HspAI GCGC 2 cut(s) 8, 202
KasI GGCGCC 1 cut(s) 201
KpnI GGTACC 1 cut(s) 255
Kzo9I GATC 2 cut(s) 607, 742
LmnI GCTCC 1 cut(s) 551
Lsp1109I GCAGC 1 cut(s) 159
MaeI CTAG 1 cut(s) 96
MaeII ACGT 3 cut(s) 501, 519, 814
MaeIII GTNAC 1 cut(s) 256
MalI GATC 2 cut(s) 609, 744
MboI GATC 2 cut(s) 607, 742
MboII GAAGA 5 cut(s) 72, 149, 152, 379, 688
MhlI GDGCHC 1 cut(s) 548
MluCI AATT 6 cut(s) 106, 217, 223, 389, 475, 514
Mly113I GGCGCC 1 cut(s) 202
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 7 cut(s) 55, 130, 205, 370, 578, 734, 756
Mph1103I ATGCAT 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 649
MspA1I CMGCKG 1 cut(s) 150
MspI CCGG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 792
MvnI CGCG 3 cut(s) 199, 321, 736
NarI GGCGCC 1 cut(s) 202
NdeI CATATG 1 cut(s) 646
NdeII GATC 2 cut(s) 607, 742
NlaIII CATG 1 cut(s) 533
NlaIV GGNNCC 4 cut(s) 203, 253, 295, 564
NmuCI GTSAC 1 cut(s) 256
NruI TCGCGA 1 cut(s) 736
NsiI ATGCAT 1 cut(s) 575
NspV TTCGAA 1 cut(s) 104
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 1 cut(s) 792
PdmI GAANNNNTTC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 679
PkrI GCNGC 4 cut(s) 149, 152, 201, 293
PleI GAGTC 1 cut(s) 253
PluTI GGCGCC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 253
Ppu21I YACGTR 1 cut(s) 520
PsiI TTATAA 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 548
PspN4I GGNNCC 4 cut(s) 203, 253, 295, 564
PspPI GGNCC 1 cut(s) 293
RruI TCGCGA 1 cut(s) 736
RsaI GTAC 2 cut(s) 253, 839
RsaNI GTAC 2 cut(s) 252, 838
RseI CAYNNNNRTG 1 cut(s) 649
SacI GAGCTC 1 cut(s) 548
SatI GCNGC 4 cut(s) 148, 151, 200, 292
Sau3AI GATC 2 cut(s) 607, 742
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 253
SduI GDGCHC 1 cut(s) 548
SfoI GGCGCC 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 649
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
SnaBI TACGTA 1 cut(s) 520
Sse9I AATT 6 cut(s) 106, 217, 223, 389, 475, 514
SsiI CCGC 3 cut(s) 150, 199, 291
SspDI GGCGCC 1 cut(s) 201
SspMI CTAG 1 cut(s) 96
SstI GAGCTC 1 cut(s) 548
TaaI ACNGT 3 cut(s) 51, 256, 806
TaiI ACGT 3 cut(s) 504, 522, 817
TaqI TCGA 1 cut(s) 104
TasI AATT 6 cut(s) 106, 217, 223, 389, 475, 514
TauI GCSGC 3 cut(s) 153, 202, 294
TfiI GAWTC 1 cut(s) 679
TscAI CASTG 1 cut(s) 54
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 1 cut(s) 147
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 2 cut(s) 17, 663
TspGWI ACGGA 2 cut(s) 385, 738
TspRI CASTG 1 cut(s) 54
XapI RAATTY 4 cut(s) 106, 217, 223, 475
XcmI CCANNNNNNNNNTGG 1 cut(s) 651
XmiI GTMKAC 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 1 cut(s) 96
Zsp2I ATGCAT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.