pycom15g03770

phospholipase d

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014680
Physical Location & Seq
Reverse (-)
2298112 .. 2301197
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g03770.7

Sequence Viewer

Length: 1560 bp
ATGAAAGTCGATCGCAGTCACAGAAGAATCACCGCCGCCGCCGTGATTGCACTCGTCGTGTTACTCAGTCTCTCTCTGCTACCCGCCACTGAATCTTCATCCCAATGCAAAGCGTGGCTCGTCCAATCCATCCCCACCGACATGCCGACTCTCCGCCGCGTCCCCGGCGTCCTCTCCACCGCGGATGTGTTGGAATGGCTGGCGAAGAACTCGACGGAGAGACTGGACGTAATTGCTCAGTACTGGCAGCTAATTGCGCAGCCTGAAGATCCGCGTTCCGGGGATTTCGGGTACTCGAAAGAGGATTTGCAGAGGTTTGGAGCTCAGGAAGGTGCTTCTGTTTATGGCGCCATAGACGATGCTGCTAATCGCAATGTTTCCATCAGGCTACTATCCCACTCAGGTGTGTATCCCGACTATACCACAGAGCCATCCAACCTTGCTTCAGGAAGGCCAAATGTAAAGAATGTGACTTTGTTGTTTAGTAAATGGTGGGGGTCCGGCGTAGTCCATGCCAAAGTCTGGATATCAGATCGCCGAGACGTGTATATTGGATCCGCCAACAATGACTGGAAATCTCTCACACAAGTGAAGGAACTTGGAATTTATCTCGTTGGTTGTCCGAAAATAGCAAGAAAGGTTGAGACCTACTTTGAAAACCTATGGACACTTGCATCTCTTGATCATACAACTCATACGACAACTGTATCAGATAAGCAGTGGCAGGTCGACAGACAGGTTCCTTGCTGGTCACACTTCTTGGATTCTAAATTGCGGTGTAGTTCACCTCTTCCTCGAACGGTGGAGACTCACCATGTAGCAGGATACCCTATACTGAACGATCCCTACATGTTCAAATTGCCACTGCAGACTCCTGGGTATAATGATTCAAGCTTGCAGCCTCAGTCCAGCTATCTCTCTTTTGCTCCTCCAGAGTTGACATTTGGTAGGTACCAGGCTGATGAACAGGCTTGGATAGAGACTATTCATTCCGTAGGAAATGGAGGAACGGTTCGGATTAGTACCATGGACTGGCTTGGTCAGTCCCAGTATACGAACCCAACGGTTTACTGGTCATCCCTCTCCTCTGCAATAACTGAGGTTGTCTTCTCAAAGAAAGCAACAGTGAAAATATTGGTAGCATACTGGGCACATTTTATAGACGGCACAGACCAGTACCTAAAATCTCTTCTCCACTCCAACGTCCTTTGCTCTTCGTCTACGTATAACCATTGCGCTGGGAAAGTTGAGATCAAGTACTATGTAGTCCCGGGTTTCAACTTGACCGGACCTGCCACTGCCAATGGTACCGACACCGGAAACATATACCCCGGTTACACCAGGGTCAACCATGGAAAGTATGCGGTCAGTGATGTTCGAGCTCACATAGGCACGAGCAATCTCATTTGGGATTACTTTTATACAACAGCTGGTGTCAGCTTTGGTACATACAACCCTGCCATTGTTTCTCAGCTTCAAGATGTTTTCGACGCCGACTGGGATTCCCCCTATGTCGTGCCAGTTGAAGAGCTGGGAGAAGGTAGGGCTTGTCCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00565 ko01100 ko01110 map00564 map00565 map01100 map01110
Pfam Domains
Protein Families
PLDc_2

Protein Analysis

520

Amino Acids

57.71

Weight (kDa)

6.16

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41120
malus_domestica MD15G1040400.v1.1
prunus_persica Prupe.1G393700_v2.0.a1 Prupe.1G393700_v2.0.a1 Prupe.1G393700_v2.0.a1
pyrus_communis pycom15g03770
rosa_chinensis RchiOBHm_Chr6g0299421
rosa_laevigata RLG00000011433
rosa_multiflora Rmu_co8364393.1_g000001
rosa_roxburghii Rroxscaffold_7G00168700
rosa_rugosa Rorug06G0288000
rosa_samantha Rh2DG624200 Rh6AG399900 Rh6BG408300 Rh6CG414000 Rh6DG401000
rosa_wichuraiana Rw6G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 258
Acc36I ACCTGC 2 cut(s) 715, 1300
Acc65I GGTACC 2 cut(s) 951, 1307
AccB1I GGYRCC 3 cut(s) 347, 951, 1307
AccB7I CCANNNNNTGG 2 cut(s) 522, 1032
AccI GTMKAC 3 cut(s) 729, 1052, 1220
AccII CGCG 3 cut(s) 159, 182, 274
AclWI GGATC 4 cut(s) 263, 549, 562, 836
AcsI RAATTY 1 cut(s) 603
AcuI CTGAAG 2 cut(s) 285, 429
AcyI GRCGYC 3 cut(s) 168, 348, 1491
AfaI GTAC 8 cut(s) 242, 293, 953, 1024, 1178, 1259, 1309, 1447
AfiI CCNNNNNNNGG 3 cut(s) 278, 522, 1032
AflIII ACRYGT 2 cut(s) 543, 849
AgsI TTSAA 6 cut(s) 656, 856, 891, 1279, 1478, 1526
AjiI CACGTC 1 cut(s) 544
AjnI CCWGG 3 cut(s) 874, 954, 1340
AleI CACNNNNGTG 2 cut(s) 402, 587
AloI GAACNNNNNNTCC 2 cut(s) 996, 1028
AluBI AGCT 9 cut(s) 250, 323, 894, 912, 1382, 1430, 1440, 1474, 1531
AluI AGCT 9 cut(s) 250, 323, 894, 912, 1382, 1430, 1440, 1474, 1531
Alw21I GWGCWC 2 cut(s) 325, 1384
Alw26I GTCTC 6 cut(s) 74, 214, 534, 638, 800, 974
AlwI GGATC 4 cut(s) 263, 549, 562, 836
Ama87I CYCGRG 1 cut(s) 1270
AoxI GGCC 1 cut(s) 452
ApeKI GCWGC 4 cut(s) 247, 259, 362, 898
ApoI RAATTY 1 cut(s) 603
Asp718I GGTACC 2 cut(s) 951, 1307
AspLEI GCGC 3 cut(s) 259, 350, 1238
AspS9I GGNCC 2 cut(s) 498, 1289
AsuC2I CCSGG 5 cut(s) 165, 280, 1271, 1272, 1332
AsuHPI GGTGA 3 cut(s) 22, 777, 803
AvaI CYCGRG 1 cut(s) 1270
AvaII GGWCC 2 cut(s) 498, 1289
BaeGI GKGCMC 1 cut(s) 1153
BaeI ACNNNNGTAYC 2 cut(s) 690, 723
BamHI GGATCC 1 cut(s) 554
BanI GGYRCC 3 cut(s) 347, 951, 1307
BanII GRGCYC 2 cut(s) 325, 1384
BauI CACGAG 1 cut(s) 1393
BbsI GAAGAC 1 cut(s) 1099
Bbv12I GWGCWC 2 cut(s) 325, 1384
BbvI GCAGC 4 cut(s) 259, 271, 349, 910
BccI CCATC 3 cut(s) 137, 389, 439
BceAI ACGGC 2 cut(s) 26, 1180
BciT130I CCWGG 3 cut(s) 876, 956, 1342
BciVI GTATCC 2 cut(s) 420, 818
BclI TGATCA 1 cut(s) 682
BcnI CCSGG 5 cut(s) 165, 280, 1271, 1272, 1332
BcoDI GTCTC 6 cut(s) 74, 214, 534, 638, 800, 974
BfmI CTRYAG 1 cut(s) 866
BfoI RGCGCY 1 cut(s) 351
BfuAI ACCTGC 2 cut(s) 715, 1300
BfuI GTATCC 2 cut(s) 420, 818
BisI GCNGC 7 cut(s) 36, 39, 157, 248, 260, 363, 899
BlsI GCNGC 7 cut(s) 37, 40, 158, 249, 261, 364, 900
BmcAI AGTACT 2 cut(s) 242, 1259
Bme1390I CCNGG 8 cut(s) 165, 280, 876, 956, 1271, 1272, 1332, 1342
Bme18I GGWCC 2 cut(s) 498, 1289
BmeT110I CYCGRG 1 cut(s) 1270
BmgBI CACGTC 1 cut(s) 544
BmgT120I GGNCC 2 cut(s) 498, 1289
BmiI GGNNCC 6 cut(s) 349, 499, 556, 741, 953, 1309
BmrFI CCNGG 8 cut(s) 165, 280, 876, 956, 1271, 1272, 1332, 1342
BmrI ACTGGG 3 cut(s) 1042, 1156, 1507
BmsI GCATC 2 cut(s) 349, 683
BmuI ACTGGG 3 cut(s) 1042, 1156, 1507
BpiI GAAGAC 1 cut(s) 1099
BpmI CTGGAG 1 cut(s) 915
Bpu10I CCTNAGC 1 cut(s) 324
BpuMI CCSGG 5 cut(s) 165, 280, 1271, 1272, 1332
BsaAI YACGTR 1 cut(s) 1224
BsaHI GRCGYC 3 cut(s) 168, 348, 1491
BsaI GGTCTC 1 cut(s) 638
BsaJI CCNNGG 9 cut(s) 163, 180, 279, 875, 1026, 1270, 1330, 1341, 1351
BsaWI WCCGGW 2 cut(s) 1286, 1316
BsaXI ACNNNNNCTCC 2 cut(s) 996, 1026
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 522, 1032
Bse3DI GCAATG 2 cut(s) 379, 1231
BseBI CCWGG 3 cut(s) 876, 956, 1342
BseDI CCNNGG 9 cut(s) 163, 180, 279, 875, 1026, 1270, 1330, 1341, 1351
BseGI GGATG 5 cut(s) 98, 129, 190, 431, 1076
BseLI CCNNNNNNNGG 3 cut(s) 278, 522, 1032
BseMI GCAATG 2 cut(s) 379, 1231
BseMII CTCAG 7 cut(s) 79, 251, 338, 414, 917, 1089, 1484
BseRI GAGGAG 2 cut(s) 918, 1075
BseSI GKGCMC 1 cut(s) 1153
BseXI GCAGC 4 cut(s) 259, 271, 349, 910
BseYI CCCAGC 2 cut(s) 1238, 1531
Bsh1236I CGCG 3 cut(s) 159, 182, 274
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 1 cut(s) 454
BshNI GGYRCC 3 cut(s) 347, 951, 1307
BsiEI CGRYCG 1 cut(s) 13
BsiHKAI GWGCWC 2 cut(s) 325, 1384
BsiHKCI CYCGRG 1 cut(s) 1270
BsiSI CCGG 7 cut(s) 165, 279, 501, 1271, 1287, 1317, 1332
BslFI GGGAC 3 cut(s) 146, 1030, 1253
BslI CCNNNNNNNGG 3 cut(s) 278, 522, 1032
BsmAI GTCTC 6 cut(s) 74, 214, 534, 638, 800, 974
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 3 cut(s) 146, 1030, 1253
BsnI GGCC 1 cut(s) 454
Bso31I GGTCTC 1 cut(s) 638
BsoBI CYCGRG 1 cut(s) 1270
Bsp1286I GDGCHC 3 cut(s) 325, 1153, 1384
Bsp143I GATC 7 cut(s) 10, 268, 532, 554, 682, 841, 1251
Bsp19I CCATGG 2 cut(s) 1026, 1351
BspANI GGCC 1 cut(s) 454
BspCNI CTCAG 7 cut(s) 78, 250, 337, 413, 916, 1090, 1483
BspFNI CGCG 3 cut(s) 159, 182, 274
BspLI GGNNCC 6 cut(s) 349, 499, 556, 741, 953, 1309
BspMAI CTGCAG 1 cut(s) 870
BspMI ACCTGC 2 cut(s) 715, 1300
BspPI GGATC 4 cut(s) 263, 549, 562, 836
BspQI GCTCTTC 2 cut(s) 1219, 1521
BspT107I GGYRCC 3 cut(s) 347, 951, 1307
BspTNI GGTCTC 1 cut(s) 638
BsrDI GCAATG 2 cut(s) 379, 1231
BssECI CCNNGG 9 cut(s) 163, 180, 279, 875, 1026, 1270, 1330, 1341, 1351
BssMI GATC 7 cut(s) 10, 268, 532, 554, 682, 841, 1251
BssNAI GTATAC 1 cut(s) 1053
BssNI GRCGYC 3 cut(s) 168, 348, 1491
BssSI CACGAG 1 cut(s) 1393
BssT1I CCWWGG 2 cut(s) 1026, 1351
Bst1107I GTATAC 1 cut(s) 1053
Bst2BI CACGAG 1 cut(s) 1393
Bst2UI CCWGG 3 cut(s) 876, 956, 1342
Bst4CI ACNGT 5 cut(s) 706, 802, 1012, 1066, 1126
Bst6I CTCTTC 4 cut(s) 795, 1194, 1219, 1521
BstACI GRCGYC 3 cut(s) 168, 348, 1491
BstBAI YACGTR 1 cut(s) 1224
BstC8I GCNNGC 2 cut(s) 201, 896
BstDEI CTNAG 7 cut(s) 65, 237, 324, 400, 903, 1098, 1470
BstDSI CCRYGG 3 cut(s) 180, 1026, 1351
BstF5I GGATG 5 cut(s) 98, 129, 190, 431, 1076
BstFNI CGCG 3 cut(s) 159, 182, 274
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 3 cut(s) 259, 350, 1238
BstKTI GATC 7 cut(s) 13, 271, 535, 557, 685, 844, 1254
BstMAI GTCTC 6 cut(s) 74, 214, 534, 638, 800, 974
BstMBI GATC 7 cut(s) 10, 268, 532, 554, 682, 841, 1251
BstMCI CGRYCG 1 cut(s) 13
BstMWI GCNNNNNNNGC 4 cut(s) 47, 165, 256, 1148
BstNI CCWGG 3 cut(s) 876, 956, 1342
BstNSI RCATGY 2 cut(s) 145, 853
BstSCI CCNGG 8 cut(s) 163, 278, 874, 954, 1269, 1270, 1330, 1340
BstSFI CTRYAG 1 cut(s) 866
BstSLI GKGCMC 1 cut(s) 1153
BstSNI TACGTA 1 cut(s) 1224
BstUI CGCG 3 cut(s) 159, 182, 274
BstV1I GCAGC 4 cut(s) 259, 271, 349, 910
BstV2I GAAGAC 1 cut(s) 1099
BstX2I RGATCY 2 cut(s) 268, 554
BstXI CCANNNNNNTGG 1 cut(s) 1238
BstYI RGATCY 2 cut(s) 268, 554
BstZ17I GTATAC 1 cut(s) 1053
BsuI GTATCC 2 cut(s) 420, 818
BsuRI GGCC 1 cut(s) 454
BtgI CCRYGG 3 cut(s) 180, 1026, 1351
BtrI CACGTC 1 cut(s) 544
BtsCI GGATG 5 cut(s) 98, 129, 190, 431, 1076
BtsI GCAGTG 3 cut(s) 725, 863, 1296
BtsIMutI CAGTG 6 cut(s) 87, 725, 863, 1131, 1296, 1375
BveI ACCTGC 2 cut(s) 715, 1300
Cac8I GCNNGC 2 cut(s) 201, 896
CfoI GCGC 3 cut(s) 259, 350, 1238
Cfr13I GGNCC 2 cut(s) 498, 1289
Cfr42I CCGCGG 1 cut(s) 183
Cfr9I CCCGGG 1 cut(s) 1270
CseI GACGC 3 cut(s) 148, 157, 1499
Csp6I GTAC 8 cut(s) 241, 292, 952, 1023, 1177, 1258, 1308, 1446
CviAII CATG 6 cut(s) 142, 512, 815, 850, 1027, 1352
CviQI GTAC 8 cut(s) 241, 292, 952, 1023, 1177, 1258, 1308, 1446
DdeI CTNAG 7 cut(s) 65, 237, 324, 400, 903, 1098, 1470
DinI GGCGCC 1 cut(s) 349
DpnI GATC 7 cut(s) 12, 270, 534, 556, 684, 843, 1253
DpnII GATC 7 cut(s) 10, 268, 532, 554, 682, 841, 1251
Eam1104I CTCTTC 4 cut(s) 795, 1194, 1219, 1521
EarI CTCTTC 4 cut(s) 795, 1194, 1219, 1521
EciI GGCGGA 2 cut(s) 143, 547
Ecl136II GAGCTC 2 cut(s) 323, 1382
Eco105I TACGTA 1 cut(s) 1224
Eco130I CCWWGG 2 cut(s) 1026, 1351
Eco24I GRGCYC 2 cut(s) 325, 1384
Eco31I GGTCTC 1 cut(s) 638
Eco32I GATATC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 498, 1289
Eco53kI GAGCTC 2 cut(s) 323, 1382
Eco57I CTGAAG 2 cut(s) 285, 429
Eco88I CYCGRG 1 cut(s) 1270
EcoICRI GAGCTC 2 cut(s) 323, 1382
EcoRII CCWGG 3 cut(s) 874, 954, 1340
EcoRV GATATC 1 cut(s) 528
EcoT14I CCWWGG 2 cut(s) 1026, 1351
EcoT38I GRGCYC 2 cut(s) 325, 1384
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
ErhI CCWWGG 2 cut(s) 1026, 1351
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 6 cut(s) 145, 515, 818, 853, 1030, 1355
FaqI GGGAC 3 cut(s) 146, 1030, 1253
FatI CATG 6 cut(s) 141, 511, 814, 849, 1026, 1351
FauI CCCGC 1 cut(s) 91
FbaI TGATCA 1 cut(s) 682
FblI GTMKAC 3 cut(s) 729, 1052, 1220
Fnu4HI GCNGC 7 cut(s) 36, 39, 157, 248, 260, 363, 899
FokI GGATG 5 cut(s) 85, 116, 197, 418, 1063
FriOI GRGCYC 2 cut(s) 325, 1384
Fsp4HI GCNGC 7 cut(s) 36, 39, 157, 248, 260, 363, 899
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 3 cut(s) 258, 349, 1237
GluI GCNGC 7 cut(s) 36, 39, 157, 248, 260, 363, 899
GsaI CCCAGC 2 cut(s) 1242, 1535
GsuI CTGGAG 1 cut(s) 915
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 1 cut(s) 454
HapII CCGG 7 cut(s) 165, 279, 501, 1271, 1287, 1317, 1332
HgaI GACGC 3 cut(s) 148, 157, 1499
HhaI GCGC 3 cut(s) 259, 350, 1238
Hin1I GRCGYC 3 cut(s) 168, 348, 1491
Hin1II CATG 6 cut(s) 145, 515, 818, 853, 1030, 1355
Hin6I GCGC 3 cut(s) 257, 348, 1236
HinP1I GCGC 3 cut(s) 257, 348, 1236
HincII GTYRAC 3 cut(s) 730, 939, 1348
HindII GTYRAC 3 cut(s) 730, 939, 1348
HindIII AAGCTT 1 cut(s) 892
HinfI GANTC 8 cut(s) 27, 92, 148, 764, 808, 871, 887, 1502
HpaII CCGG 7 cut(s) 165, 279, 501, 1271, 1287, 1317, 1332
HphI GGTGA 3 cut(s) 22, 777, 803
Hpy166II GTNNAC 7 cut(s) 730, 785, 939, 1053, 1069, 1221, 1348
Hpy188I TCNGA 4 cut(s) 532, 624, 712, 1017
Hpy188III TCNNGA 7 cut(s) 326, 413, 447, 523, 680, 932, 1478
Hpy8I GTNNAC 7 cut(s) 730, 785, 939, 1053, 1069, 1221, 1348
Hpy99I CGWCG 3 cut(s) 59, 217, 1493
HpyAV CCTTC 4 cut(s) 323, 444, 586, 1532
HpyCH4III ACNGT 5 cut(s) 706, 802, 1012, 1066, 1126
HpyCH4IV ACGT 4 cut(s) 228, 543, 1203, 1223
HpyCH4V TGCA 7 cut(s) 50, 108, 310, 674, 868, 898, 1091
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 165, 256, 1148
HpyF3I CTNAG 7 cut(s) 65, 237, 324, 400, 903, 1098, 1470
HpySE526I ACGT 4 cut(s) 228, 543, 1203, 1223
Hsp92I GRCGYC 3 cut(s) 168, 348, 1491
Hsp92II CATG 6 cut(s) 145, 515, 818, 853, 1030, 1355
HspAI GCGC 3 cut(s) 257, 348, 1236
KasI GGCGCC 1 cut(s) 347
KpnI GGTACC 2 cut(s) 955, 1311
Ksp22I TGATCA 1 cut(s) 682
KspI CCGCGG 1 cut(s) 183
Kzo9I GATC 7 cut(s) 10, 268, 532, 554, 682, 841, 1251
LguI GCTCTTC 2 cut(s) 1219, 1521
LmnI GCTCC 2 cut(s) 320, 931
Lsp1109I GCAGC 4 cut(s) 259, 271, 349, 910
LweI GCATC 2 cut(s) 349, 683
MaeII ACGT 4 cut(s) 228, 543, 1203, 1223
MaeIII GTNAC 5 cut(s) 17, 60, 469, 750, 1334
MalI GATC 7 cut(s) 12, 270, 534, 556, 684, 843, 1253
MboI GATC 7 cut(s) 10, 268, 532, 554, 682, 841, 1251
MboII GAAGA 9 cut(s) 36, 87, 217, 278, 782, 1099, 1181, 1206, 1538
MflI RGATCY 2 cut(s) 268, 554
MhlI GDGCHC 3 cut(s) 325, 1153, 1384
MluCI AATT 5 cut(s) 231, 252, 603, 770, 857
Mly113I GGCGCC 1 cut(s) 348
MlyI GAGTC 3 cut(s) 142, 802, 865
MmeI TCCRAC 3 cut(s) 171, 459, 1224
MslI CAYNNNNRTG 4 cut(s) 103, 140, 402, 587
MspA1I CMGCKG 2 cut(s) 182, 1430
MspI CCGG 7 cut(s) 165, 279, 501, 1271, 1287, 1317, 1332
MspR9I CCNGG 8 cut(s) 165, 280, 876, 956, 1271, 1272, 1332, 1342
MvaI CCWGG 3 cut(s) 876, 956, 1342
MvnI CGCG 3 cut(s) 159, 182, 274
MwoI GCNNNNNNNGC 4 cut(s) 47, 165, 256, 1148
NarI GGCGCC 1 cut(s) 348
NciI CCSGG 5 cut(s) 165, 280, 1271, 1272, 1332
NcoI CCATGG 2 cut(s) 1026, 1351
NdeII GATC 7 cut(s) 10, 268, 532, 554, 682, 841, 1251
NlaIII CATG 6 cut(s) 145, 515, 818, 853, 1030, 1355
NlaIV GGNNCC 6 cut(s) 349, 499, 556, 741, 953, 1309
NmeAIII GCCGAG 1 cut(s) 563
NmuCI GTSAC 3 cut(s) 17, 469, 750
NsbI TGCGCA 1 cut(s) 258
NspI RCATGY 2 cut(s) 145, 853
OliI CACNNNNGTG 2 cut(s) 402, 587
PciI ACATGT 1 cut(s) 849
PciSI GCTCTTC 2 cut(s) 1219, 1521
PfeI GAWTC 5 cut(s) 27, 92, 764, 887, 1502
PflMI CCANNNNNTGG 2 cut(s) 522, 1032
PkrI GCNGC 7 cut(s) 37, 40, 158, 249, 261, 364, 900
Ple19I CGATCG 1 cut(s) 13
PleI GAGTC 3 cut(s) 142, 802, 865
PluTI GGCGCC 1 cut(s) 351
PpsI GAGTC 3 cut(s) 142, 802, 865
Ppu21I YACGTR 1 cut(s) 1224
PscI ACATGT 1 cut(s) 849
Psp124BI GAGCTC 2 cut(s) 325, 1384
Psp6I CCWGG 3 cut(s) 874, 954, 1340
PspFI CCCAGC 2 cut(s) 1238, 1531
PspGI CCWGG 3 cut(s) 874, 954, 1340
PspN4I GGNNCC 6 cut(s) 349, 499, 556, 741, 953, 1309
PspPI GGNCC 2 cut(s) 498, 1289
PsrI GAACNNNNNNTAC 2 cut(s) 830, 862
PstI CTGCAG 1 cut(s) 870
PsuI RGATCY 2 cut(s) 268, 554
PvuI CGATCG 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 1430
RsaI GTAC 8 cut(s) 242, 293, 953, 1024, 1178, 1259, 1309, 1447
RsaNI GTAC 8 cut(s) 241, 292, 952, 1023, 1177, 1258, 1308, 1446
RseI CAYNNNNRTG 4 cut(s) 103, 140, 402, 587
SacI GAGCTC 2 cut(s) 325, 1384
SacII CCGCGG 1 cut(s) 183
SalI GTCGAC 1 cut(s) 728
SapI GCTCTTC 2 cut(s) 1219, 1521
SatI GCNGC 7 cut(s) 36, 39, 157, 248, 260, 363, 899
Sau3AI GATC 7 cut(s) 10, 268, 532, 554, 682, 841, 1251
Sau96I GGNCC 2 cut(s) 498, 1289
ScaI AGTACT 2 cut(s) 242, 1259
SchI GAGTC 3 cut(s) 142, 802, 865
ScrFI CCNGG 8 cut(s) 165, 280, 876, 956, 1271, 1272, 1332, 1342
SduI GDGCHC 3 cut(s) 325, 1153, 1384
SfaNI GCATC 2 cut(s) 349, 683
SfcI CTRYAG 1 cut(s) 866
SfoI GGCGCC 1 cut(s) 349
Sfr303I CCGCGG 1 cut(s) 183
SgrBI CCGCGG 1 cut(s) 183
SinI GGWCC 2 cut(s) 498, 1289
SmaI CCCGGG 1 cut(s) 1272
SmiMI CAYNNNNRTG 4 cut(s) 103, 140, 402, 587
SnaBI TACGTA 1 cut(s) 1224
Sse9I AATT 5 cut(s) 231, 252, 603, 770, 857
SspDI GGCGCC 1 cut(s) 347
SspI AATATT 1 cut(s) 1134
SstI GAGCTC 2 cut(s) 325, 1384
StyD4I CCNGG 8 cut(s) 163, 278, 874, 954, 1269, 1270, 1330, 1340
StyI CCWWGG 2 cut(s) 1026, 1351
TaaI ACNGT 5 cut(s) 706, 802, 1012, 1066, 1126
TaiI ACGT 4 cut(s) 231, 546, 1206, 1226
TaqI TCGA 7 cut(s) 9, 212, 296, 729, 796, 1378, 1488
TasI AATT 5 cut(s) 231, 252, 603, 770, 857
TatI WGTACW 2 cut(s) 240, 1257
TauI GCSGC 3 cut(s) 38, 41, 159
TfiI GAWTC 5 cut(s) 27, 92, 764, 887, 1502
TscAI CASTG 6 cut(s) 94, 725, 870, 1131, 1303, 1375
TseFI GTSAC 3 cut(s) 17, 469, 750
TseI GCWGC 4 cut(s) 247, 259, 362, 898
Tsp45I GTSAC 3 cut(s) 17, 469, 750
TspDTI ATGAA 4 cut(s) 17, 87, 977, 978
TspGWI ACGGA 2 cut(s) 230, 982
TspMI CCCGGG 1 cut(s) 1270
TspRI CASTG 6 cut(s) 94, 725, 870, 1131, 1303, 1375
Van91I CCANNNNNTGG 2 cut(s) 522, 1032
VpaK11BI GGWCC 2 cut(s) 498, 1289
XapI RAATTY 1 cut(s) 603
XceI RCATGY 2 cut(s) 145, 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 1068
XmaI CCCGGG 1 cut(s) 1270
XmiI GTMKAC 3 cut(s) 729, 1052, 1220
ZrmI AGTACT 2 cut(s) 242, 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.