Rh2DG624200

phospholipase d

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014680
Physical Location & Seq
Forward (+)
85413518 .. 85417578
4061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG624200.1

Sequence Viewer

Length: 1305 bp
ATGTTGTTAAAGTGGATACCTCCTGATGCTGAAATTTACAATTTTCTGGTTCAGGGTCATTACAAGGTTAATGACTTAAAGAAAGTTTGGCAGCTATTTTGTGTCATGACAAGAAAAGGTTTCAACATTACACTCACAACTTACCACAATTTGGTGCGTTTGAAGTGTCTGGAAGGTAGGGTTCTTCATGCATGGAAATTGACTGAGCTTATGATTGGACAAACTGATCCTCATGATCTCAGCATCTACAATATTCTGATATTCTATATTTTCCCAACAGGGAATACTTTGTTTGTCAAGAAAGTTGTGGAGCATTTGCAAGAGAAGAAATTGCTAATTGATGAACTCCAGGTTCGGCGAAATGCAAAGCATGGCTGGTCCAATCAATCCCCACCGACATGCCGGGTCTCCGTCGTGTTCCTGGTGTCCTCTTCACCGAGACTGGACATAATTGCTCAGTACTGGCAGTTTGTAGCCAATCCTAAAGACCCTCAATCTGGAGATTTTGGATACTCAAAACAGGACATGAAGAGATTTGGAGCTCCTGAAGGTGCTTCCATTTATGGCGCCATAGATGATGCTGCTAATCGCAATCTTACTATCAGGCTGCTATCACATTCGGGTGTATATCCCGACTACACCAAAGAGCCTTCTAAACTTGCTTCAGGAAGGCCCAATGTGAAGAATGTCACTTTGTTACTTAGTGAATGGTGGGGTTCTGGCATAGTCCATGCCAAAGTTTGGATATCAGATCGGCGAGATGTGTATATTGGGTCTGCAAACAATGACTGGAAATCTCTCACACAAGTCAAGGAAGTTGGAATTTATCTTGCTGATTGTCCAAAAATAGCGAGAAAGGTTGAGACCTACTTTGACAACCTGTGGAAACTTGCATCTCTTAATTCTACAGCTTACACAACAAATGTATCAGATAAACAGTGGCAGATCGAGAGACAAGTTCCTTGCTGGTCGCATTTTGTGGATTCTAAAGCAAGGTGTAGTTCACCTCTCCCTGGGATTGTGGAGACTGACCATGTAGCAGGATACCCCATACTGTCAGACCCTGATATGTTCAAAATGCAGCTCCAGACTCCTGGACATAATGATTCAAGTTTGCAACCCCAATCCAGCTATCTATCTTTTGCTCATCCAGAGCTGTCATTTGGCAAGTACCAGGCAGATGAACAAGCATGGATAGAGACTATTAAATCTGTAGGAAGTGGAGGGACTGTCAGGATTAGTACCATGGACCTGTTATCCAGTGTGATTAGGATCGTCTGCAATTATCAACTAGTCCATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00565 ko01100 ko01110 map00564 map00565 map01100 map01110
Pfam Domains
Protein Families
PLDc_2

Protein Analysis

434

Amino Acids

49.38

Weight (kDa)

8.75

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 8 - 52 4.3e-07 PPR repeat family
PLDc_2 PF13091 185 - 295 3.9e-11 PLD-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41120
malus_domestica MD15G1040400.v1.1
prunus_persica Prupe.1G393700_v2.0.a1 Prupe.1G393700_v2.0.a1 Prupe.1G393700_v2.0.a1
pyrus_communis pycom15g03770
rosa_chinensis RchiOBHm_Chr6g0299421
rosa_laevigata RLG00000011433
rosa_multiflora Rmu_co8364393.1_g000001
rosa_roxburghii Rroxscaffold_7G00168700
rosa_rugosa Rorug06G0288000
rosa_samantha Rh2DG624200 Rh6AG399900 Rh6BG408300 Rh6CG414000 Rh6DG401000
rosa_wichuraiana Rw6G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 566
AccB7I CCANNNNNTGG 2 cut(s) 151, 741
AclWI GGATC 2 cut(s) 221, 1280
AcsI RAATTY 2 cut(s) 33, 822
AcuI CTGAAG 2 cut(s) 567, 648
AcyI GRCGYC 1 cut(s) 567
AfaI GTAC 3 cut(s) 461, 1172, 1243
AfiI CCNNNNNNNGG 4 cut(s) 151, 497, 741, 1013
AgsI TTSAA 4 cut(s) 124, 163, 1075, 1110
AhlI ACTAGT 1 cut(s) 1291
AjnI CCWGG 5 cut(s) 348, 420, 1012, 1093, 1173
AluBI AGCT 7 cut(s) 94, 208, 542, 911, 1084, 1131, 1156
AluI AGCT 7 cut(s) 94, 208, 542, 911, 1084, 1131, 1156
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 6 cut(s) 412, 433, 857, 946, 1019, 1193
AlwI GGATC 2 cut(s) 221, 1280
AlwNI CAGNNNCTG 1 cut(s) 1064
AoxI GGCC 1 cut(s) 671
ApeKI GCWGC 4 cut(s) 91, 581, 607, 1081
ApoI RAATTY 2 cut(s) 33, 822
AspLEI GCGC 1 cut(s) 569
AspS9I GGNCC 3 cut(s) 378, 672, 1249
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 2 cut(s) 426, 996
AvaII GGWCC 2 cut(s) 378, 1249
BaeI ACNNNNGTAYC 2 cut(s) 909, 942
BanI GGYRCC 1 cut(s) 566
BanII GRGCYC 1 cut(s) 544
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 4 cut(s) 103, 568, 594, 1093
BciT130I CCWGG 5 cut(s) 350, 422, 1014, 1095, 1175
BciVI GTATCC 3 cut(s) 9, 503, 1037
BcnI CCSGG 1 cut(s) 404
BcoDI GTCTC 6 cut(s) 412, 433, 857, 946, 1019, 1193
BcuI ACTAGT 1 cut(s) 1291
BfaI CTAG 2 cut(s) 1292, 1303
BfmI CTRYAG 2 cut(s) 906, 1212
BfoI RGCGCY 1 cut(s) 570
BfuI GTATCC 3 cut(s) 9, 503, 1037
BisI GCNGC 4 cut(s) 92, 582, 608, 1082
BlsI GCNGC 4 cut(s) 93, 583, 609, 1083
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 6 cut(s) 350, 404, 422, 1014, 1095, 1175
Bme18I GGWCC 2 cut(s) 378, 1249
BmgT120I GGNCC 3 cut(s) 378, 672, 1249
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 6 cut(s) 350, 404, 422, 1014, 1095, 1175
BmsI GCATC 4 cut(s) 16, 252, 568, 902
BpmI CTGGAG 3 cut(s) 332, 519, 1070
BpuMI CCSGG 1 cut(s) 404
BsaBI GATNNNNATC 1 cut(s) 1271
BsaHI GRCGYC 1 cut(s) 567
BsaI GGTCTC 2 cut(s) 412, 857
BsaJI CCNNGG 3 cut(s) 1012, 1013, 1245
BsaXI ACNNNNNCTCC 2 cut(s) 1215, 1245
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 497, 741, 1013
Bse1I ACTGG 4 cut(s) 447, 467, 794, 1260
Bse8I GATNNNNATC 1 cut(s) 1271
BseBI CCWGG 5 cut(s) 350, 422, 1014, 1095, 1175
BseDI CCNNGG 3 cut(s) 1012, 1013, 1245
BseGI GGATG 1 cut(s) 1147
BseJI GATNNNNATC 1 cut(s) 1271
BseLI CCNNNNNNNGG 4 cut(s) 151, 497, 741, 1013
BseMII CTCAG 3 cut(s) 195, 253, 470
BseNI ACTGG 4 cut(s) 447, 467, 794, 1260
BseXI GCAGC 4 cut(s) 103, 568, 594, 1093
BshFI GGCC 1 cut(s) 673
BshNI GGYRCC 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 544
BsiSI CCGG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 1240
BslI CCNNNNNNNGG 4 cut(s) 151, 497, 741, 1013
BsmAI GTCTC 6 cut(s) 412, 433, 857, 946, 1019, 1193
BsmFI GGGAC 1 cut(s) 1240
BsnI GGCC 1 cut(s) 673
Bso31I GGTCTC 2 cut(s) 412, 857
Bsp1286I GDGCHC 1 cut(s) 544
Bsp143I GATC 5 cut(s) 226, 235, 751, 945, 1272
Bsp19I CCATGG 1 cut(s) 1245
BspANI GGCC 1 cut(s) 673
BspCNI CTCAG 3 cut(s) 196, 252, 469
BspHI TCATGA 2 cut(s) 105, 232
BspLI GGNNCC 1 cut(s) 568
BspPI GGATC 2 cut(s) 221, 1280
BspT107I GGYRCC 1 cut(s) 566
BspTNI GGTCTC 2 cut(s) 412, 857
BsrI ACTGG 4 cut(s) 447, 467, 794, 1260
BssECI CCNNGG 3 cut(s) 1012, 1013, 1245
BssMI GATC 5 cut(s) 226, 235, 751, 945, 1272
BssNI GRCGYC 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 1245
Bst2UI CCWGG 5 cut(s) 350, 422, 1014, 1095, 1175
Bst4CI ACNGT 3 cut(s) 939, 1056, 1231
Bst6I CTCTTC 2 cut(s) 436, 524
BstACI GRCGYC 1 cut(s) 567
BstDEI CTNAG 4 cut(s) 204, 239, 456, 701
BstDSI CCRYGG 1 cut(s) 1245
BstF5I GGATG 1 cut(s) 1147
BstH2I RGCGCY 1 cut(s) 570
BstHHI GCGC 1 cut(s) 569
BstKTI GATC 5 cut(s) 229, 238, 754, 948, 1275
BstMAI GTCTC 6 cut(s) 412, 433, 857, 946, 1019, 1193
BstMBI GATC 5 cut(s) 226, 235, 751, 945, 1272
BstNI CCWGG 5 cut(s) 350, 422, 1014, 1095, 1175
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 6 cut(s) 348, 402, 420, 1012, 1093, 1173
BstSFI CTRYAG 2 cut(s) 906, 1212
BstV1I GCAGC 4 cut(s) 103, 568, 594, 1093
BstXI CCANNNNNNTGG 1 cut(s) 1094
BsuI GTATCC 3 cut(s) 9, 503, 1037
BsuRI GGCC 1 cut(s) 673
BtgI CCRYGG 1 cut(s) 1245
BtsCI GGATG 1 cut(s) 1147
BtsIMutI CAGTG 2 cut(s) 944, 1267
CaiI CAGNNNCTG 1 cut(s) 1064
CciI TCATGA 2 cut(s) 105, 232
CfoI GCGC 1 cut(s) 569
Cfr13I GGNCC 3 cut(s) 378, 672, 1249
Csp6I GTAC 3 cut(s) 460, 1171, 1242
CviQI GTAC 3 cut(s) 460, 1171, 1242
DdeI CTNAG 4 cut(s) 204, 239, 456, 701
DinI GGCGCC 1 cut(s) 568
DpnI GATC 5 cut(s) 228, 237, 753, 947, 1274
DpnII GATC 5 cut(s) 226, 235, 751, 945, 1272
Eam1104I CTCTTC 2 cut(s) 436, 524
EarI CTCTTC 2 cut(s) 436, 524
Ecl136II GAGCTC 1 cut(s) 542
Eco130I CCWWGG 1 cut(s) 1245
Eco24I GRGCYC 1 cut(s) 544
Eco31I GGTCTC 2 cut(s) 412, 857
Eco32I GATATC 1 cut(s) 747
Eco47I GGWCC 2 cut(s) 378, 1249
Eco53kI GAGCTC 1 cut(s) 542
Eco57I CTGAAG 2 cut(s) 567, 648
EcoICRI GAGCTC 1 cut(s) 542
EcoRII CCWGG 5 cut(s) 348, 420, 1012, 1093, 1173
EcoRV GATATC 1 cut(s) 747
EcoT14I CCWWGG 1 cut(s) 1245
EcoT22I ATGCAT 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 544
EgeI GGCGCC 1 cut(s) 568
EheI GGCGCC 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 1245
FaqI GGGAC 1 cut(s) 1240
Fnu4HI GCNGC 4 cut(s) 92, 582, 608, 1082
FokI GGATG 1 cut(s) 1134
FriOI GRGCYC 1 cut(s) 544
Fsp4HI GCNGC 4 cut(s) 92, 582, 608, 1082
FspBI CTAG 2 cut(s) 1292, 1303
GlaI GCGC 1 cut(s) 568
GluI GCNGC 4 cut(s) 92, 582, 608, 1082
GsuI CTGGAG 3 cut(s) 332, 519, 1070
HaeII RGCGCY 1 cut(s) 570
HaeIII GGCC 1 cut(s) 673
HapII CCGG 1 cut(s) 403
HhaI GCGC 1 cut(s) 569
Hin1I GRCGYC 1 cut(s) 567
Hin6I GCGC 1 cut(s) 567
HinP1I GCGC 1 cut(s) 567
HinfI GANTC 3 cut(s) 983, 1090, 1106
HpaII CCGG 1 cut(s) 403
HphI GGTGA 2 cut(s) 426, 996
Hpy166II GTNNAC 1 cut(s) 1004
Hpy188I TCNGA 4 cut(s) 258, 751, 931, 1060
Hpy8I GTNNAC 1 cut(s) 1004
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 4 cut(s) 167, 542, 660, 663
HpyCH4III ACNGT 3 cut(s) 939, 1056, 1231
HpyCH4V TGCA 8 cut(s) 191, 319, 365, 779, 893, 1081, 1117, 1281
HpyF3I CTNAG 4 cut(s) 204, 239, 456, 701
Hsp92I GRCGYC 1 cut(s) 567
HspAI GCGC 1 cut(s) 567
KasI GGCGCC 1 cut(s) 566
Kzo9I GATC 5 cut(s) 226, 235, 751, 945, 1272
LmnI GCTCC 4 cut(s) 310, 539, 547, 1089
Lsp1109I GCAGC 4 cut(s) 103, 568, 594, 1093
LweI GCATC 4 cut(s) 16, 252, 568, 902
MaeI CTAG 2 cut(s) 1292, 1303
MaeIII GTNAC 2 cut(s) 688, 696
MalI GATC 5 cut(s) 228, 237, 753, 947, 1274
MboI GATC 5 cut(s) 226, 235, 751, 945, 1272
MboII GAAGA 5 cut(s) 176, 337, 423, 541, 694
MhlI GDGCHC 1 cut(s) 544
Mly113I GGCGCC 1 cut(s) 567
MlyI GAGTC 1 cut(s) 1084
MmeI TCCRAC 1 cut(s) 799
MnlI CCTC 6 cut(s) 30, 240, 439, 501, 1017, 1217
Mph1103I ATGCAT 1 cut(s) 193
MseI TTAA 5 cut(s) 8, 69, 77, 900, 1206
MslI CAYNNNNRTG 2 cut(s) 397, 621
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 6 cut(s) 350, 404, 422, 1014, 1095, 1175
MvaI CCWGG 5 cut(s) 350, 422, 1014, 1095, 1175
NarI GGCGCC 1 cut(s) 567
NciI CCSGG 1 cut(s) 404
NcoI CCATGG 1 cut(s) 1245
NdeII GATC 5 cut(s) 226, 235, 751, 945, 1272
NlaIV GGNNCC 1 cut(s) 568
NmuCI GTSAC 1 cut(s) 688
NsiI ATGCAT 1 cut(s) 193
NspI RCATGY 1 cut(s) 402
PagI TCATGA 2 cut(s) 105, 232
PasI CCCWGGG 1 cut(s) 1013
PfeI GAWTC 2 cut(s) 983, 1106
PflMI CCANNNNNTGG 2 cut(s) 151, 741
PfoI TCCNGGA 1 cut(s) 1093
PkrI GCNGC 4 cut(s) 93, 583, 609, 1083
PleI GAGTC 1 cut(s) 1084
PluTI GGCGCC 1 cut(s) 570
PpsI GAGTC 1 cut(s) 1084
Psp124BI GAGCTC 1 cut(s) 544
Psp6I CCWGG 5 cut(s) 348, 420, 1012, 1093, 1173
PspGI CCWGG 5 cut(s) 348, 420, 1012, 1093, 1173
PspN4I GGNNCC 1 cut(s) 568
PspPI GGNCC 3 cut(s) 378, 672, 1249
PstNI CAGNNNCTG 1 cut(s) 1064
RsaI GTAC 3 cut(s) 461, 1172, 1243
RsaNI GTAC 3 cut(s) 460, 1171, 1242
RseI CAYNNNNRTG 2 cut(s) 397, 621
SacI GAGCTC 1 cut(s) 544
SaqAI TTAA 5 cut(s) 8, 69, 77, 900, 1206
SatI GCNGC 4 cut(s) 92, 582, 608, 1082
Sau3AI GATC 5 cut(s) 226, 235, 751, 945, 1272
Sau96I GGNCC 3 cut(s) 378, 672, 1249
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 1 cut(s) 1084
ScrFI CCNGG 6 cut(s) 350, 404, 422, 1014, 1095, 1175
SduI GDGCHC 1 cut(s) 544
SfaNI GCATC 4 cut(s) 16, 252, 568, 902
SfcI CTRYAG 2 cut(s) 906, 1212
SfoI GGCGCC 1 cut(s) 568
SinI GGWCC 2 cut(s) 378, 1249
SmiMI CAYNNNNRTG 2 cut(s) 397, 621
SpeI ACTAGT 1 cut(s) 1291
SspDI GGCGCC 1 cut(s) 566
SspI AATATT 1 cut(s) 253
SspMI CTAG 2 cut(s) 1292, 1303
SstI GAGCTC 1 cut(s) 544
StyD4I CCNGG 6 cut(s) 348, 402, 420, 1012, 1093, 1173
StyI CCWWGG 1 cut(s) 1245
TaaI ACNGT 3 cut(s) 939, 1056, 1231
TaqI TCGA 1 cut(s) 948
TatI WGTACW 1 cut(s) 459
TfiI GAWTC 2 cut(s) 983, 1106
Tru1I TTAA 5 cut(s) 8, 69, 77, 900, 1206
Tru9I TTAA 5 cut(s) 8, 69, 77, 900, 1206
TscAI CASTG 2 cut(s) 944, 1267
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 4 cut(s) 91, 581, 607, 1081
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 4 cut(s) 176, 357, 542, 1197
TspGWI ACGGA 1 cut(s) 400
TspRI CASTG 2 cut(s) 944, 1267
Van91I CCANNNNNTGG 2 cut(s) 151, 741
VpaK11BI GGWCC 2 cut(s) 378, 1249
XapI RAATTY 2 cut(s) 33, 822
XceI RCATGY 1 cut(s) 402
XspI CTAG 2 cut(s) 1292, 1303
ZrmI AGTACT 1 cut(s) 461
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.