Rroxscaffold_7G00168700

phospholipase d

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014680
Physical Location & Seq
Reverse (-)
9528557 .. 9531001
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00168700.1

Sequence Viewer

Length: 1389 bp
ATGAAAGTCATTCACAGTCGCATTATCGTCGTCATCGTTTGGGTTTTAGTCAACCTTTCTTTGCTACCCGTCTCTGATTGTTCAGGTTCGGCGAAATGCAAAGCATGGCTGGTCCAATCAATCCCCACCGACATGCCGGGTCTCCGTCGTGTCCCTGGTGTCCTCTCCACCGCGGATGTGTTCCGGTGGCTGGCGCGGAACTCATCACAGAGACTGGACATAATTGCTCAGTACTGGCAGTTTGTTGCCAATCCTAAAGACCCTCAATCTGGAGATTTTGGATACTCAAAACAGGACATGAAGAGATTTGGAGCTCACCAAGGTGTATATCCTGACTACACCAAAGAGCCTTCCAAACTTGCTTCAGGAAGGCCCAATGTGAAGAATGTCACTTTGTTACTTAGTGAATGGTGGGGTTCTGGGCATAGTCCATGCCAAAGTTTGGATATCAGATCGGCGAGATGTGTCAAGGAAGTTGGAATTTATCTTGCTGGTTGTCCAAAAATAGCGAGAAAGGTTGAGACCTACTTTGACAACCTGTGGAAACTTGCATCTCTTAGTTCTACAGCTTACACAACAAATGTATCAGATAAACAGTGGCAGACCGAGAGACAAGTTCCTTGCTGGTCGCATTTCGTGGATTCTAAAGCAAGGTGTAGTTCACCTCTCCCTGGGATTGTGGAGACTGACCATGTAGCAGGATACCCCATACTGTCAGACCCTGATATGTTCAAAATGCAGCTCCAGACTCCTGGACGTAATGATTCAAGTTTGCAACCCCAATCCAGCTATCTGTCTTTTTGCTCCTCCAGAGGTAATTGGCCACTGTCATTTGGCAAGTACCAGGCAGATGAACAAGCATGGATAGAGACTATTAAATCTGTAGGAAGTGGAGGGACTGTCAGGATTAGTACCATGGACTGGCTTGGTCAGTCCCAGTTTATGAAGCATACAATTTATTGGGGGCATCCCTCTCCTCTGCAATATCAGAGCACAGATCAATACCTGAAATCTCTCCTCTACTCTAATGTTCTCTGCAATTCATCCAAGTATAACCACTGTTCTGGGAAAGTTGAGATCAAGTATTATATGGTCCCAGGTTTCAACTCGACCGGACCTGCCAGAATTGGCAATGGTACTCATACTGGAAATATATACCCTGGTTACACCAGGGTTAACCATGGCAAGTATGCAGTAAGTGATGTTCGAGCCCATATTGGGACGAGCAATCTTGTGTGGGATTACTTTTATACAACAGCGGGTGTCAGCTTTGGTACAAATAACTCTGCCATTGTTTCACAACTTCAACAAGTCTTTGATGCTGACTGGGATTCGGAATATGCTGTGCCAGTTGATGAGTTGGGGGAGGGTAATGCTTTTTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00565 ko01100 ko01110 map00564 map00565 map01100 map01110
Pfam Domains
Protein Families
PLDc_2

Protein Analysis

462

Amino Acids

51.51

Weight (kDa)

8.64

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41120
malus_domestica MD15G1040400.v1.1
prunus_persica Prupe.1G393700_v2.0.a1 Prupe.1G393700_v2.0.a1 Prupe.1G393700_v2.0.a1
pyrus_communis pycom15g03770
rosa_chinensis RchiOBHm_Chr6g0299421
rosa_laevigata RLG00000011433
rosa_multiflora Rmu_co8364393.1_g000001
rosa_roxburghii Rroxscaffold_7G00168700
rosa_rugosa Rorug06G0288000
rosa_samantha Rh2DG624200 Rh6AG399900 Rh6BG408300 Rh6CG414000 Rh6DG401000
rosa_wichuraiana Rw6G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1126
AccB7I CCANNNNNTGG 2 cut(s) 442, 921
AccII CGCG 2 cut(s) 173, 196
AciI CCGC 4 cut(s) 171, 173, 196, 1259
AcoI YGGCCR 1 cut(s) 821
AcsI RAATTY 1 cut(s) 480
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 5 cut(s) 233, 842, 913, 1138, 1276
AfiI CCNNNNNNNGG 6 cut(s) 190, 269, 442, 671, 921, 1218
AgsI TTSAA 5 cut(s) 733, 768, 1105, 1307, 1383
AjnI CCWGG 7 cut(s) 154, 670, 751, 843, 1096, 1159, 1169
AleI CACNNNNGTG 1 cut(s) 321
AluBI AGCT 6 cut(s) 314, 569, 742, 789, 1269, 1386
AluI AGCT 6 cut(s) 314, 569, 742, 789, 1269, 1386
Alw21I GWGCWC 2 cut(s) 316, 995
Alw26I GTCTC 7 cut(s) 76, 146, 205, 515, 604, 677, 863
AlwNI CAGNNNCTG 2 cut(s) 214, 722
AoxI GGCC 2 cut(s) 371, 821
ApeKI GCWGC 1 cut(s) 739
ApoI RAATTY 1 cut(s) 480
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 4 cut(s) 112, 372, 1093, 1115
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 2 cut(s) 308, 654
AvaII GGWCC 3 cut(s) 112, 1093, 1115
BaeI ACNNNNGTAYC 2 cut(s) 567, 600
BalI TGGCCA 1 cut(s) 823
BanII GRGCYC 2 cut(s) 316, 1213
Bbv12I GWGCWC 2 cut(s) 316, 995
BbvI GCAGC 1 cut(s) 751
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 7 cut(s) 156, 672, 753, 845, 1098, 1161, 1171
BciVI GTATCC 2 cut(s) 275, 695
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 7 cut(s) 76, 146, 205, 515, 604, 677, 863
BfmI CTRYAG 2 cut(s) 564, 882
BfuAI ACCTGC 1 cut(s) 1126
BfuI GTATCC 2 cut(s) 275, 695
BisI GCNGC 1 cut(s) 740
BlsI GCNGC 1 cut(s) 741
BmcAI AGTACT 1 cut(s) 233
Bme1390I CCNGG 8 cut(s) 138, 156, 672, 753, 845, 1098, 1161, 1171
Bme18I GGWCC 3 cut(s) 112, 1093, 1115
BmgT120I GGNCC 4 cut(s) 112, 372, 1093, 1115
BmiI GGNNCC 1 cut(s) 1095
BmrFI CCNGG 8 cut(s) 138, 156, 672, 753, 845, 1098, 1161, 1171
BmrI ACTGGG 2 cut(s) 931, 1336
BmsI GCATC 3 cut(s) 560, 976, 1309
BmuI ACTGGG 2 cut(s) 931, 1336
BpmI CTGGAG 3 cut(s) 291, 728, 793
BpuMI CCSGG 1 cut(s) 138
BsaI GGTCTC 2 cut(s) 146, 515
BsaWI WCCGGW 2 cut(s) 183, 1112
BsaXI ACNNNNNCTCC 2 cut(s) 885, 915
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 269, 442, 671, 921, 1218
Bse1I ACTGG 7 cut(s) 219, 239, 926, 937, 1150, 1331, 1349
Bse3DI GCAATG 1 cut(s) 1138
BseBI CCWGG 7 cut(s) 156, 672, 753, 845, 1098, 1161, 1171
BseGI GGATG 3 cut(s) 181, 967, 1043
BseLI CCNNNNNNNGG 6 cut(s) 190, 269, 442, 671, 921, 1218
BseMI GCAATG 1 cut(s) 1138
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 7 cut(s) 219, 239, 926, 937, 1150, 1331, 1349
BseRI GAGGAG 3 cut(s) 796, 966, 1007
BseXI GCAGC 1 cut(s) 751
Bsh1236I CGCG 2 cut(s) 173, 196
Bsh1285I CGRYCG 1 cut(s) 1113
BshFI GGCC 2 cut(s) 373, 823
BsiEI CGRYCG 1 cut(s) 1113
BsiHKAI GWGCWC 2 cut(s) 316, 995
BsiSI CCGG 3 cut(s) 137, 184, 1113
BslFI GGGAC 5 cut(s) 137, 910, 919, 1079, 1234
BslI CCNNNNNNNGG 6 cut(s) 190, 269, 442, 671, 921, 1218
BsmAI GTCTC 7 cut(s) 76, 146, 205, 515, 604, 677, 863
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 5 cut(s) 137, 910, 919, 1079, 1234
BsnI GGCC 2 cut(s) 373, 823
Bso31I GGTCTC 2 cut(s) 146, 515
Bsp1286I GDGCHC 3 cut(s) 316, 995, 1213
Bsp143I GATC 3 cut(s) 452, 997, 1077
Bsp19I CCATGG 2 cut(s) 915, 1180
BspACI CCGC 4 cut(s) 171, 173, 196, 1259
BspANI GGCC 2 cut(s) 373, 823
BspCNI CTCAG 1 cut(s) 241
BspFNI CGCG 2 cut(s) 173, 196
BspLI GGNNCC 1 cut(s) 1095
BspMI ACCTGC 1 cut(s) 1126
BspTNI GGTCTC 2 cut(s) 146, 515
BsrDI GCAATG 1 cut(s) 1138
BsrI ACTGG 7 cut(s) 219, 239, 926, 937, 1150, 1331, 1349
BssMI GATC 3 cut(s) 452, 997, 1077
BssT1I CCWWGG 3 cut(s) 319, 915, 1180
Bst2UI CCWGG 7 cut(s) 156, 672, 753, 845, 1098, 1161, 1171
Bst4CI ACNGT 6 cut(s) 17, 597, 714, 828, 901, 1061
Bst6I CTCTTC 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 3 cut(s) 228, 401, 557
BstDSI CCRYGG 3 cut(s) 171, 915, 1180
BstF5I GGATG 3 cut(s) 181, 967, 1043
BstFNI CGCG 2 cut(s) 173, 196
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 3 cut(s) 455, 1000, 1080
BstMAI GTCTC 7 cut(s) 76, 146, 205, 515, 604, 677, 863
BstMBI GATC 3 cut(s) 452, 997, 1077
BstMCI CGRYCG 1 cut(s) 1113
BstNI CCWGG 7 cut(s) 156, 672, 753, 845, 1098, 1161, 1171
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 8 cut(s) 136, 154, 670, 751, 843, 1096, 1159, 1169
BstSFI CTRYAG 2 cut(s) 564, 882
BstUI CGCG 2 cut(s) 173, 196
BstV1I GCAGC 1 cut(s) 751
BstXI CCANNNNNNTGG 2 cut(s) 752, 1064
BsuI GTATCC 2 cut(s) 275, 695
BsuRI GGCC 2 cut(s) 373, 823
BtgI CCRYGG 3 cut(s) 171, 915, 1180
BtsCI GGATG 3 cut(s) 181, 967, 1043
BtsIMutI CAGTG 3 cut(s) 602, 824, 1057
BveI ACCTGC 1 cut(s) 1126
Cac8I GCNNGC 1 cut(s) 192
CaiI CAGNNNCTG 2 cut(s) 214, 722
CfoI GCGC 1 cut(s) 196
Cfr13I GGNCC 4 cut(s) 112, 372, 1093, 1115
Cfr42I CCGCGG 1 cut(s) 174
Csp6I GTAC 5 cut(s) 232, 841, 912, 1137, 1275
CviAII CATG 8 cut(s) 105, 133, 298, 432, 692, 861, 916, 1181
CviQI GTAC 5 cut(s) 232, 841, 912, 1137, 1275
DdeI CTNAG 3 cut(s) 228, 401, 557
DpnI GATC 3 cut(s) 454, 999, 1079
DpnII GATC 3 cut(s) 452, 997, 1077
EaeI YGGCCR 1 cut(s) 821
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 3 cut(s) 319, 915, 1180
Eco24I GRGCYC 2 cut(s) 316, 1213
Eco31I GGTCTC 2 cut(s) 146, 515
Eco32I GATATC 1 cut(s) 448
Eco47I GGWCC 3 cut(s) 112, 1093, 1115
Eco53kI GAGCTC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 348
EcoICRI GAGCTC 1 cut(s) 314
EcoRII CCWGG 7 cut(s) 154, 670, 751, 843, 1096, 1159, 1169
EcoRV GATATC 1 cut(s) 448
EcoT14I CCWWGG 3 cut(s) 319, 915, 1180
EcoT38I GRGCYC 2 cut(s) 316, 1213
ErhI CCWWGG 3 cut(s) 319, 915, 1180
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 8 cut(s) 108, 136, 301, 435, 695, 864, 919, 1184
FaqI GGGAC 5 cut(s) 137, 910, 919, 1079, 1234
FatI CATG 8 cut(s) 104, 132, 297, 431, 691, 860, 915, 1180
FauI CCCGC 1 cut(s) 1252
Fnu4HI GCNGC 1 cut(s) 740
FokI GGATG 3 cut(s) 188, 954, 1030
FriOI GRGCYC 2 cut(s) 316, 1213
Fsp4HI GCNGC 1 cut(s) 740
GlaI GCGC 1 cut(s) 195
GluI GCNGC 1 cut(s) 740
GsuI CTGGAG 3 cut(s) 291, 728, 793
HaeIII GGCC 2 cut(s) 373, 823
HapII CCGG 3 cut(s) 137, 184, 1113
HhaI GCGC 1 cut(s) 196
Hin1II CATG 8 cut(s) 108, 136, 301, 435, 695, 864, 919, 1184
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HincII GTYRAC 2 cut(s) 52, 1177
HindII GTYRAC 2 cut(s) 52, 1177
HinfI GANTC 4 cut(s) 641, 748, 764, 1331
HpaI GTTAAC 1 cut(s) 1177
HpaII CCGG 3 cut(s) 137, 184, 1113
HphI GGTGA 2 cut(s) 308, 654
Hpy166II GTNNAC 3 cut(s) 52, 662, 1177
Hpy188I TCNGA 6 cut(s) 76, 452, 589, 718, 990, 1336
Hpy188III TCNNGA 6 cut(s) 270, 332, 366, 745, 810, 904
Hpy8I GTNNAC 3 cut(s) 52, 662, 1177
Hpy99I CGWCG 2 cut(s) 32, 150
HpyAV CCTTC 2 cut(s) 360, 363
HpyCH4III ACNGT 6 cut(s) 17, 597, 714, 828, 901, 1061
HpyCH4IV ACGT 1 cut(s) 757
HpyCH4V TGCA 7 cut(s) 99, 551, 739, 775, 982, 1038, 1193
HpyF3I CTNAG 3 cut(s) 228, 401, 557
HpySE526I ACGT 1 cut(s) 757
Hsp92II CATG 8 cut(s) 108, 136, 301, 435, 695, 864, 919, 1184
HspAI GCGC 1 cut(s) 194
KspAI GTTAAC 1 cut(s) 1177
KspI CCGCGG 1 cut(s) 174
Kzo9I GATC 3 cut(s) 452, 997, 1077
LmnI GCTCC 3 cut(s) 311, 747, 809
Lsp1109I GCAGC 1 cut(s) 751
LweI GCATC 3 cut(s) 560, 976, 1309
MaeII ACGT 1 cut(s) 757
MaeIII GTNAC 3 cut(s) 388, 396, 1163
MalI GATC 3 cut(s) 454, 999, 1079
MboI GATC 3 cut(s) 452, 997, 1077
MboII GAAGA 2 cut(s) 313, 394
MhlI GDGCHC 3 cut(s) 316, 995, 1213
MlsI TGGCCA 1 cut(s) 823
MluCI AATT 6 cut(s) 222, 480, 817, 954, 1039, 1125
MluNI TGGCCA 1 cut(s) 823
MlyI GAGTC 1 cut(s) 742
MmeI TCCRAC 1 cut(s) 457
Mox20I TGGCCA 1 cut(s) 823
MscI TGGCCA 1 cut(s) 823
MseI TTAA 2 cut(s) 876, 1176
MslI CAYNNNNRTG 2 cut(s) 131, 321
Msp20I TGGCCA 1 cut(s) 823
MspA1I CMGCKG 2 cut(s) 173, 1259
MspI CCGG 3 cut(s) 137, 184, 1113
MspR9I CCNGG 8 cut(s) 138, 156, 672, 753, 845, 1098, 1161, 1171
MvaI CCWGG 7 cut(s) 156, 672, 753, 845, 1098, 1161, 1171
MvnI CGCG 2 cut(s) 173, 196
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 2 cut(s) 915, 1180
NdeII GATC 3 cut(s) 452, 997, 1077
NlaIII CATG 8 cut(s) 108, 136, 301, 435, 695, 864, 919, 1184
NlaIV GGNNCC 1 cut(s) 1095
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 136
OliI CACNNNNGTG 1 cut(s) 321
PasI CCCWGGG 1 cut(s) 671
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PfeI GAWTC 3 cut(s) 641, 764, 1331
PflMI CCANNNNNTGG 2 cut(s) 442, 921
PfoI TCCNGGA 1 cut(s) 751
PkrI GCNGC 1 cut(s) 741
PleI GAGTC 1 cut(s) 742
PpsI GAGTC 1 cut(s) 742
Psp124BI GAGCTC 1 cut(s) 316
Psp6I CCWGG 7 cut(s) 154, 670, 751, 843, 1096, 1159, 1169
PspGI CCWGG 7 cut(s) 154, 670, 751, 843, 1096, 1159, 1169
PspN4I GGNNCC 1 cut(s) 1095
PspPI GGNCC 4 cut(s) 112, 372, 1093, 1115
PsrI GAACNNNNNNTAC 2 cut(s) 1188, 1220
PstNI CAGNNNCTG 2 cut(s) 214, 722
RsaI GTAC 5 cut(s) 233, 842, 913, 1138, 1276
RsaNI GTAC 5 cut(s) 232, 841, 912, 1137, 1275
RseI CAYNNNNRTG 2 cut(s) 131, 321
SacI GAGCTC 1 cut(s) 316
SacII CCGCGG 1 cut(s) 174
SaqAI TTAA 2 cut(s) 876, 1176
SatI GCNGC 1 cut(s) 740
Sau3AI GATC 3 cut(s) 452, 997, 1077
Sau96I GGNCC 4 cut(s) 112, 372, 1093, 1115
ScaI AGTACT 1 cut(s) 233
SchI GAGTC 1 cut(s) 742
ScrFI CCNGG 8 cut(s) 138, 156, 672, 753, 845, 1098, 1161, 1171
SduI GDGCHC 3 cut(s) 316, 995, 1213
SfaNI GCATC 3 cut(s) 560, 976, 1309
SfcI CTRYAG 2 cut(s) 564, 882
Sfr303I CCGCGG 1 cut(s) 174
SgrBI CCGCGG 1 cut(s) 174
SinI GGWCC 3 cut(s) 112, 1093, 1115
SmiMI CAYNNNNRTG 2 cut(s) 131, 321
Sse9I AATT 6 cut(s) 222, 480, 817, 954, 1039, 1125
SsiI CCGC 4 cut(s) 171, 173, 196, 1259
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 8 cut(s) 136, 154, 670, 751, 843, 1096, 1159, 1169
StyI CCWWGG 3 cut(s) 319, 915, 1180
TaaI ACNGT 6 cut(s) 17, 597, 714, 828, 901, 1061
TaiI ACGT 1 cut(s) 760
TaqI TCGA 2 cut(s) 1109, 1207
TaqII GACCGA 1 cut(s) 620
TasI AATT 6 cut(s) 222, 480, 817, 954, 1039, 1125
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 3 cut(s) 641, 764, 1331
Tru1I TTAA 2 cut(s) 876, 1176
Tru9I TTAA 2 cut(s) 876, 1176
TscAI CASTG 3 cut(s) 602, 831, 1064
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 1 cut(s) 739
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 5 cut(s) 17, 314, 867, 959, 1032
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 3 cut(s) 602, 831, 1064
Van91I CCANNNNNTGG 2 cut(s) 442, 921
VpaK11BI GGWCC 3 cut(s) 112, 1093, 1115
XapI RAATTY 1 cut(s) 480
XceI RCATGY 1 cut(s) 136
ZrmI AGTACT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.