Rmu_co8364393.1_g000001

phospholipase d

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8364393.1
OG0014680
Physical Location & Seq
Forward (+)
10 .. 1094
1085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8364393.1_g000001.1.cds

Sequence Viewer

Length: 639 bp
atgaagagatttggagctcaccaaggtgcttccgtttatggcgccatagatgatgctgctaatcgcaatcttactatcaggctactatcacactcaggtgtatatcctgactacaccaaagagccttccaaacttgcttcaggaaggcccaatgtgaagaatgtcactttgttactaagtgaatggtggggttctggcatagtccatgccaaagtttggatatcagatcggcgagatgtatatattgggtctgcaaacaatgactggaaatctctcacacaagtcaaggaagttggaatttatcttgctggttgtccaaaaatagcgagaaaggttgagacctactttgacaacctgtggaaactcgcatctcttaattctacagcttacacaacaaatgtatcagataaacagtggcagaccgagagacaagttccttgctggtcgcattttgtggattctaaagcaaggtgtagttcacctctccctgggattgtggagactgaccatgtagcaggataccccatactgtcagaccctgatatgttcaaaatgcagctccagactcctggacataatgattcaagtttgcaaccccaatccagctatctatcttttgctcctccagaggtaattggc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00565 ko01100 ko01110 map00564 map00565 map01100 map01110
Pfam Domains
Protein Families
PLDc_2

Protein Analysis

213

Amino Acids

23.7

Weight (kDa)

8.37

Isoelectric Point (pI)

31.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41120
malus_domestica MD15G1040400.v1.1
prunus_persica Prupe.1G393700_v2.0.a1 Prupe.1G393700_v2.0.a1 Prupe.1G393700_v2.0.a1
pyrus_communis pycom15g03770
rosa_chinensis RchiOBHm_Chr6g0299421
rosa_laevigata RLG00000011433
rosa_multiflora Rmu_co8364393.1_g000001
rosa_roxburghii Rroxscaffold_7G00168700
rosa_rugosa Rorug06G0288000
rosa_samantha Rh2DG624200 Rh6AG399900 Rh6BG408300 Rh6CG414000 Rh6DG401000
rosa_wichuraiana Rw6G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AccB7I CCANNNNNTGG 1 cut(s) 216
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 42
AfiI CCNNNNNNNGG 2 cut(s) 216, 488
AgsI TTSAA 2 cut(s) 550, 585
AjnI CCWGG 2 cut(s) 487, 568
AleI CACNNNNGTG 2 cut(s) 24, 96
AluBI AGCT 4 cut(s) 17, 386, 559, 606
AluI AGCT 4 cut(s) 17, 386, 559, 606
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 3 cut(s) 332, 421, 494
AlwNI CAGNNNCTG 1 cut(s) 539
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 2 cut(s) 56, 556
ApoI RAATTY 1 cut(s) 297
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 147
AsuHPI GGTGA 2 cut(s) 11, 471
BaeI ACNNNNGTAYC 2 cut(s) 384, 417
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 43, 568
BciT130I CCWGG 2 cut(s) 489, 570
BciVI GTATCC 1 cut(s) 512
BcoDI GTCTC 3 cut(s) 332, 421, 494
BfmI CTRYAG 1 cut(s) 381
BfoI RGCGCY 1 cut(s) 45
BfuI GTATCC 1 cut(s) 512
BisI GCNGC 2 cut(s) 57, 557
BlsI GCNGC 2 cut(s) 58, 558
Bme1390I CCNGG 2 cut(s) 489, 570
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 2 cut(s) 489, 570
BmsI GCATC 2 cut(s) 43, 377
BpmI CTGGAG 2 cut(s) 545, 609
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 3 cut(s) 22, 487, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 488
Bse1I ACTGG 1 cut(s) 269
BseBI CCWGG 2 cut(s) 489, 570
BseDI CCNNGG 3 cut(s) 22, 487, 488
BseLI CCNNNNNNNGG 2 cut(s) 216, 488
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 1 cut(s) 612
BseXI GCAGC 2 cut(s) 43, 568
BshFI GGCC 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 19
BslI CCNNNNNNNGG 2 cut(s) 216, 488
BsmAI GTCTC 3 cut(s) 332, 421, 494
BsnI GGCC 1 cut(s) 148
Bso31I GGTCTC 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 1 cut(s) 226
BspANI GGCC 1 cut(s) 148
BspCNI CTCAG 1 cut(s) 107
BspLI GGNNCC 1 cut(s) 43
BspT107I GGYRCC 1 cut(s) 41
BspTNI GGTCTC 1 cut(s) 332
BsrI ACTGG 1 cut(s) 269
BssECI CCNNGG 3 cut(s) 22, 487, 488
BssMI GATC 1 cut(s) 226
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 22
Bst2UI CCWGG 2 cut(s) 489, 570
Bst4CI ACNGT 2 cut(s) 414, 531
BstACI GRCGYC 1 cut(s) 42
BstDEI CTNAG 2 cut(s) 94, 176
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 3 cut(s) 332, 421, 494
BstMBI GATC 1 cut(s) 226
BstNI CCWGG 2 cut(s) 489, 570
BstSCI CCNGG 2 cut(s) 487, 568
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 2 cut(s) 43, 568
BstXI CCANNNNNNTGG 1 cut(s) 569
BsuI GTATCC 1 cut(s) 512
BsuRI GGCC 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 419
CaiI CAGNNNCTG 1 cut(s) 539
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 147
CviAII CATG 2 cut(s) 206, 509
CviJI RGCY 7 cut(s) 17, 82, 124, 148, 386, 559, 606
CviKI_1 RGCY 7 cut(s) 17, 82, 124, 148, 386, 559, 606
DdeI CTNAG 2 cut(s) 94, 176
DinI GGCGCC 1 cut(s) 43
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 332
Eco32I GATATC 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 123
EcoICRI GAGCTC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 487, 568
EcoRV GATATC 1 cut(s) 222
EcoT14I CCWWGG 1 cut(s) 22
EcoT38I GRGCYC 1 cut(s) 19
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 2 cut(s) 209, 512
FatI CATG 2 cut(s) 205, 508
Fnu4HI GCNGC 2 cut(s) 57, 557
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 2 cut(s) 57, 557
GlaI GCGC 1 cut(s) 43
GluI GCNGC 2 cut(s) 57, 557
GsuI CTGGAG 2 cut(s) 545, 609
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 1 cut(s) 148
HhaI GCGC 1 cut(s) 44
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 2 cut(s) 209, 512
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HinfI GANTC 3 cut(s) 458, 565, 581
HphI GGTGA 2 cut(s) 11, 471
Hpy166II GTNNAC 1 cut(s) 479
Hpy188I TCNGA 3 cut(s) 226, 406, 535
Hpy188III TCNNGA 4 cut(s) 107, 141, 562, 626
Hpy8I GTNNAC 1 cut(s) 479
HpyAV CCTTC 2 cut(s) 135, 138
HpyCH4III ACNGT 2 cut(s) 414, 531
HpyCH4V TGCA 3 cut(s) 254, 556, 592
HpyF3I CTNAG 2 cut(s) 94, 176
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 2 cut(s) 209, 512
HspAI GCGC 1 cut(s) 42
KasI GGCGCC 1 cut(s) 41
Kzo9I GATC 1 cut(s) 226
LmnI GCTCC 3 cut(s) 14, 564, 625
Lsp1109I GCAGC 2 cut(s) 43, 568
LweI GCATC 2 cut(s) 43, 377
MaeIII GTNAC 2 cut(s) 163, 171
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 2 cut(s) 16, 169
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 3 cut(s) 297, 376, 633
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 1 cut(s) 559
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 3 cut(s) 492, 622, 633
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 2 cut(s) 24, 96
MspR9I CCNGG 2 cut(s) 489, 570
MvaI CCWGG 2 cut(s) 489, 570
NarI GGCGCC 1 cut(s) 42
NdeII GATC 1 cut(s) 226
NlaIII CATG 2 cut(s) 209, 512
NlaIV GGNNCC 1 cut(s) 43
NmuCI GTSAC 1 cut(s) 163
OliI CACNNNNGTG 2 cut(s) 24, 96
PasI CCCWGGG 1 cut(s) 488
PfeI GAWTC 2 cut(s) 458, 581
PflMI CCANNNNNTGG 1 cut(s) 216
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 2 cut(s) 58, 558
PleI GAGTC 1 cut(s) 559
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 559
Psp124BI GAGCTC 1 cut(s) 19
Psp6I CCWGG 2 cut(s) 487, 568
PspGI CCWGG 2 cut(s) 487, 568
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 1 cut(s) 147
PstNI CAGNNNCTG 1 cut(s) 539
RseI CAYNNNNRTG 2 cut(s) 24, 96
SacI GAGCTC 1 cut(s) 19
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 2 cut(s) 57, 557
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 1 cut(s) 147
SchI GAGTC 1 cut(s) 559
ScrFI CCNGG 2 cut(s) 489, 570
SduI GDGCHC 1 cut(s) 19
SfaNI GCATC 2 cut(s) 43, 377
SfcI CTRYAG 1 cut(s) 381
SfoI GGCGCC 1 cut(s) 43
SmiMI CAYNNNNRTG 2 cut(s) 24, 96
Sse9I AATT 3 cut(s) 297, 376, 633
SspDI GGCGCC 1 cut(s) 41
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 2 cut(s) 487, 568
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 2 cut(s) 414, 531
TaqII GACCGA 1 cut(s) 437
TasI AATT 3 cut(s) 297, 376, 633
TfiI GAWTC 2 cut(s) 458, 581
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 1 cut(s) 419
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 2 cut(s) 56, 556
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 419
Van91I CCANNNNNTGG 1 cut(s) 216
XapI RAATTY 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.