pycom15g05780

PHAX RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008081
Physical Location & Seq
Reverse (-)
3501733 .. 3504138
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 780 bp
ATGGAAGGAGAAGACAACGTATTGGATGCAGTATTTGAAGATGATGACTTCATGGATGGTGGGGATGTTGAGATGCTCGATGTCGAAGAAGGAGAGCTTGTGGATTTCAATTCACAAAGTGGGTATGGTTCGGGGCAGGGCAGTGGTGGCAATGTCAGTGTAGCAAACCAAGAACGTCAGGGCCCGAATCAGAAGCCCAAGCCAAAGACGAAGAAAAAGAAGAACAAGAAGAGGAATAACGGCCATTGGACTGCGAGTATTGGGACTGACATTGACAGGTTTGTGATAGAGACATGTAAGCGACTGAAGGAGAGGAAATCGTATTTGGTATATACTGCTGTTGGTTGCTTGGGTATTGCTGCATTGAGTGATCTCATTAAAGAGGTGGATGCAATCCAGGCATGCGGAGGTCAGATGACTTCTGATGGCAGACGTTTCCGATATGGTGGTGGGATACTTTGGAATATCATTAAATCACGCGAACCAAAGGCCTATAGAGAGATAATGAAAAAAGCAAGGGACTTTGAGAAGCAATTCAAGAAACCAAATAGACCAGCAATAGAGCAAAGGAAAGAAGGCTCATCTGCCAATTCATCCAAGGATGGTACCCCAGCCAATGTGTCGGATGATTTACCGGTCCTACATGAACTGCAGACTCAAGCCAATCAGTCAAACCCCGAAGTGAAACACGTTTCTGTTCGTGATAGGTTAAGGTTGCCTGTTTCATATGATGATGACCTGCTTGGAGGGGAGCCCAAAGACGATCCACTTAATTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RNA_GG_bind

Protein Analysis

260

Amino Acids

28.74

Weight (kDa)

5.8

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHAX_RNA-bd PF10258 95 - 173 3.7e-24 Phosphorylated adapter RNA export protein, RNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 747
Acc65I GGTACC 1 cut(s) 605
AccB1I GGYRCC 1 cut(s) 605
AccII CGCG 1 cut(s) 480
AciI CCGC 1 cut(s) 405
AclWI GGATC 1 cut(s) 758
AcoI YGGCCR 1 cut(s) 241
AcuI CTGAAG 1 cut(s) 326
AdeI CACNNNGTG 1 cut(s) 119
AfaI GTAC 1 cut(s) 607
AflIII ACRYGT 2 cut(s) 293, 688
AgeI ACCGGT 1 cut(s) 634
AgsI TTSAA 3 cut(s) 38, 109, 538
AjnI CCWGG 1 cut(s) 396
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 1 cut(s) 758
AoxI GGCC 3 cut(s) 181, 241, 489
ApaI GGGCCC 1 cut(s) 185
ApeKI GCWGC 1 cut(s) 359
AsiGI ACCGGT 1 cut(s) 634
Asp700I GAANNNNTTC 1 cut(s) 533
Asp718I GGTACC 1 cut(s) 605
AspS9I GGNCC 3 cut(s) 181, 182, 637
AvaII GGWCC 1 cut(s) 637
BaeGI GKGCMC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 605
BanII GRGCYC 2 cut(s) 185, 756
BbsI GAAGAC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 346
BccI CCATC 3 cut(s) 50, 419, 596
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 1 cut(s) 398
BciVI GTATCC 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 284
BfmI CTRYAG 2 cut(s) 493, 650
BfuAI ACCTGC 1 cut(s) 747
BfuI GTATCC 1 cut(s) 447
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 637
BmgT120I GGNCC 3 cut(s) 181, 182, 637
BmiI GGNNCC 3 cut(s) 183, 607, 753
BmrFI CCNGG 1 cut(s) 398
BmsI GCATC 3 cut(s) 16, 63, 379
BpiI GAAGAC 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 642
BsaJI CCNNGG 1 cut(s) 597
BsaWI WCCGGW 1 cut(s) 634
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bse118I RCCGGY 1 cut(s) 634
Bse3DI GCAATG 1 cut(s) 157
BseBI CCWGG 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 597
BseGI GGATG 7 cut(s) 31, 61, 70, 394, 593, 607, 631
BseMI GCAATG 1 cut(s) 157
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 1 cut(s) 346
BseYI CCCAGC 1 cut(s) 610
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 3 cut(s) 183, 243, 491
BshNI GGYRCC 1 cut(s) 605
BshTI ACCGGT 1 cut(s) 634
BsiSI CCGG 1 cut(s) 635
BslFI GGGAC 2 cut(s) 277, 533
BsmAI GTCTC 1 cut(s) 284
BsmFI GGGAC 2 cut(s) 277, 533
BsnI GGCC 3 cut(s) 183, 243, 491
Bsp120I GGGCCC 1 cut(s) 181
Bsp1286I GDGCHC 2 cut(s) 185, 756
Bsp143I GATC 2 cut(s) 370, 763
BspACI CCGC 1 cut(s) 405
BspANI GGCC 3 cut(s) 183, 243, 491
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 3 cut(s) 183, 607, 753
BspMAI CTGCAG 1 cut(s) 654
BspMI ACCTGC 1 cut(s) 747
BspPI GGATC 1 cut(s) 758
BspT107I GGYRCC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 157
BsrFI RCCGGY 1 cut(s) 634
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 2 cut(s) 370, 763
BssT1I CCWWGG 1 cut(s) 597
Bst2UI CCWGG 1 cut(s) 398
Bst6I CTCTTC 1 cut(s) 224
BstC8I GCNNGC 1 cut(s) 403
BstF5I GGATG 7 cut(s) 31, 61, 70, 394, 593, 607, 631
BstFNI CGCG 1 cut(s) 480
BstKTI GATC 2 cut(s) 373, 766
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 2 cut(s) 370, 763
BstMWI GCNNNNNNNGC 2 cut(s) 147, 398
BstNI CCWGG 1 cut(s) 398
BstNSI RCATGY 2 cut(s) 297, 405
BstSCI CCNGG 1 cut(s) 396
BstSFI CTRYAG 2 cut(s) 493, 650
BstSLI GKGCMC 1 cut(s) 185
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 1 cut(s) 346
BstV2I GAAGAC 1 cut(s) 18
BsuI GTATCC 1 cut(s) 447
BsuRI GGCC 3 cut(s) 183, 243, 491
BtsCI GGATG 7 cut(s) 31, 61, 70, 394, 593, 607, 631
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 2 cut(s) 148, 163
BveI ACCTGC 1 cut(s) 747
Cac8I GCNNGC 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 3 cut(s) 181, 182, 637
Csp6I GTAC 1 cut(s) 606
CspAI ACCGGT 1 cut(s) 634
CviAII CATG 4 cut(s) 52, 294, 402, 644
CviQI GTAC 1 cut(s) 606
DpnI GATC 2 cut(s) 372, 765
DpnII GATC 2 cut(s) 370, 763
DraIII CACNNNGTG 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 597
Eco147I AGGCCT 1 cut(s) 491
Eco24I GRGCYC 2 cut(s) 185, 756
Eco47I GGWCC 1 cut(s) 637
Eco57I CTGAAG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 396
EcoT14I CCWWGG 1 cut(s) 597
EcoT38I GRGCYC 2 cut(s) 185, 756
ErhI CCWWGG 1 cut(s) 597
FaeI CATG 4 cut(s) 55, 297, 405, 647
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 2 cut(s) 277, 533
FatI CATG 4 cut(s) 51, 293, 401, 643
FauNDI CATATG 1 cut(s) 727
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 7 cut(s) 38, 68, 77, 401, 580, 614, 638
FriOI GRGCYC 2 cut(s) 185, 756
Fsp4HI GCNGC 1 cut(s) 360
GluI GCNGC 1 cut(s) 360
GsaI CCCAGC 1 cut(s) 614
HaeIII GGCC 3 cut(s) 183, 243, 491
HapII CCGG 1 cut(s) 635
Hin1II CATG 4 cut(s) 55, 297, 405, 647
HinfI GANTC 2 cut(s) 187, 655
HpaII CCGG 1 cut(s) 635
Hpy188I TCNGA 5 cut(s) 192, 414, 424, 440, 625
Hpy188III TCNNGA 2 cut(s) 538, 701
HpyAV CCTTC 3 cut(s) 83, 301, 569
HpyCH4IV ACGT 4 cut(s) 18, 175, 433, 690
HpyCH4V TGCA 4 cut(s) 29, 362, 392, 652
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 398
HpySE526I ACGT 4 cut(s) 18, 175, 433, 690
Hsp92II CATG 4 cut(s) 55, 297, 405, 647
KpnI GGTACC 1 cut(s) 609
Kzo9I GATC 2 cut(s) 370, 763
LmnI GCTCC 1 cut(s) 751
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 3 cut(s) 16, 63, 379
MaeII ACGT 4 cut(s) 18, 175, 433, 690
MalI GATC 2 cut(s) 372, 765
MboI GATC 2 cut(s) 370, 763
MboII GAAGA 6 cut(s) 23, 50, 98, 223, 232, 241
MhlI GDGCHC 2 cut(s) 185, 756
MluCI AATT 4 cut(s) 109, 533, 589, 772
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 603
MnlI CCTC 5 cut(s) 225, 306, 376, 401, 740
MroXI GAANNNNTTC 1 cut(s) 533
MseI TTAA 4 cut(s) 378, 471, 710, 771
MspI CCGG 1 cut(s) 635
MspR9I CCNGG 1 cut(s) 398
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 2 cut(s) 147, 398
NdeI CATATG 1 cut(s) 727
NdeII GATC 2 cut(s) 370, 763
NlaIII CATG 4 cut(s) 55, 297, 405, 647
NlaIV GGNNCC 3 cut(s) 183, 607, 753
NspI RCATGY 2 cut(s) 297, 405
PaeI GCATGC 1 cut(s) 405
PceI AGGCCT 1 cut(s) 491
PciI ACATGT 1 cut(s) 293
PdmI GAANNNNTTC 1 cut(s) 533
PfeI GAWTC 1 cut(s) 187
PinAI ACCGGT 1 cut(s) 634
PkrI GCNGC 1 cut(s) 361
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
PscI ACATGT 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 396
PspFI CCCAGC 1 cut(s) 610
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 3 cut(s) 183, 607, 753
PspOMI GGGCCC 1 cut(s) 181
PspPI GGNCC 3 cut(s) 181, 182, 637
PstI CTGCAG 1 cut(s) 654
RsaI GTAC 1 cut(s) 607
RsaNI GTAC 1 cut(s) 606
SaqAI TTAA 4 cut(s) 378, 471, 710, 771
SatI GCNGC 1 cut(s) 360
Sau3AI GATC 2 cut(s) 370, 763
Sau96I GGNCC 3 cut(s) 181, 182, 637
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 2 cut(s) 185, 756
SfaNI GCATC 3 cut(s) 16, 63, 379
SfcI CTRYAG 2 cut(s) 493, 650
SinI GGWCC 1 cut(s) 637
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
SphI GCATGC 1 cut(s) 405
Sse9I AATT 4 cut(s) 109, 533, 589, 772
SseBI AGGCCT 1 cut(s) 491
SsiI CCGC 1 cut(s) 405
StuI AGGCCT 1 cut(s) 491
StyD4I CCNGG 1 cut(s) 396
StyI CCWWGG 1 cut(s) 597
TaiI ACGT 4 cut(s) 21, 178, 436, 693
TaqI TCGA 2 cut(s) 78, 84
TasI AATT 4 cut(s) 109, 533, 589, 772
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 4 cut(s) 378, 471, 710, 771
Tru9I TTAA 4 cut(s) 378, 471, 710, 771
TscAI CASTG 2 cut(s) 148, 163
TseI GCWGC 1 cut(s) 359
TspDTI ATGAA 5 cut(s) 40, 521, 582, 660, 714
TspRI CASTG 2 cut(s) 148, 163
VpaK11BI GGWCC 1 cut(s) 637
XceI RCATGY 2 cut(s) 297, 405
XmnI GAANNNNTTC 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.