RLG00000010478

PHAX RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008081
Physical Location & Seq
Reverse (-)
1982695 .. 1984894
2200 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010478

Sequence Viewer

Length: 801 bp
ATGGAAGGAGAAGATAGCGTATTGGATTCAATATATGATGATAACCTTGAGGATGGTGAGGATGTTGAAATGCTCGATGTCGAAGAAGGGGAGCTTGTGGAGCATGAATCACAAAATGGCTCAGTACAGGGGAGTGGTGGGAATGACAGTGGAGCAAACCAAGAGCATCAGAGCAAGGGTAGCAAGAATAAGAGGAAGAAGAGGAGAAATAAGAGAAAGAACAGCAGCGGCGGCCCATGGACTAATGTATTTGACATAGACAGGTTTGTTTCGGAGACATGTAGAAATTTGAGAGAGAAAAAATCATATATGGTATACACTGCTGTTGGTTGTCTTGGTGTTGCTGCATTGTCTGATCTCATCAAAGAGGTGAATGCAATCCAGGCATGTGGAGGTCAGATGACTGCTAATGGCAGACGTTTGCGGACAGGTGGTGGTCTGCTGTGGAATATCATCAAAGCACGAGAGCCAAAGGCCTATAAAGAGATCATGAAGAAAGCCAAGGAATTTGAGAAGCAATTCAAGCAACCATATAATAGACAACCACCAACAAACCAAAGTAAAGAAGAACCATCCCAAGTACTTGCTACTTCATCAGACGATGCGACTCCAGCCAAATTTTCAAATGATTTGCTGCATGTATCTGAAATGACTGAAATGACCGAAATGCCTGAAGTTCAGACTCGAGACAATCAGTCAAATACCCGAAGTAAACACATTTCTGTTCATGATAGGCTAAGGGTACCTGTTTCATATGATGATGACCTGCTTACAGGGGAACCCAAAGATGATGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RNA_GG_bind

Protein Analysis

267

Amino Acids

29.77

Weight (kDa)

5.33

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHAX_RNA-bd PF10258 89 - 169 1.4e-24 Phosphorylated adapter RNA export protein, RNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 774
Acc65I GGTACC 1 cut(s) 742
AccB1I GGYRCC 1 cut(s) 742
AccI GTMKAC 1 cut(s) 315
AciI CCGC 3 cut(s) 228, 231, 424
AcsI RAATTY 3 cut(s) 286, 506, 617
AcuI CTGAAG 1 cut(s) 693
AfaI GTAC 3 cut(s) 126, 582, 744
AflIII ACRYGT 1 cut(s) 278
AgsI TTSAA 4 cut(s) 30, 68, 523, 624
AhdI GACNNNNNGTC 1 cut(s) 694
AjnI CCWGG 1 cut(s) 381
AluBI AGCT 1 cut(s) 94
AluI AGCT 1 cut(s) 94
Alw26I GTCTC 2 cut(s) 269, 681
Ama87I CYCGRG 1 cut(s) 684
AoxI GGCC 2 cut(s) 232, 474
ApeKI GCWGC 3 cut(s) 225, 344, 634
ApoI RAATTY 3 cut(s) 286, 506, 617
Asp700I GAANNNNTTC 1 cut(s) 518
Asp718I GGTACC 1 cut(s) 742
AspS9I GGNCC 1 cut(s) 233
AsuHPI GGTGA 2 cut(s) 68, 382
AvaI CYCGRG 1 cut(s) 684
BanI GGYRCC 1 cut(s) 742
BauI CACGAG 1 cut(s) 462
BbvI GCAGC 3 cut(s) 237, 331, 621
BccI CCATC 2 cut(s) 47, 580
BciT130I CCWGG 1 cut(s) 383
BcoDI GTCTC 2 cut(s) 269, 681
BfuAI ACCTGC 1 cut(s) 774
BisI GCNGC 5 cut(s) 226, 229, 232, 345, 635
BlsI GCNGC 5 cut(s) 227, 230, 233, 346, 636
BmcAI AGTACT 1 cut(s) 582
Bme1390I CCNGG 1 cut(s) 383
BmeRI GACNNNNNGTC 1 cut(s) 694
BmeT110I CYCGRG 1 cut(s) 684
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 2 cut(s) 744, 780
BmrFI CCNGG 1 cut(s) 383
BmsI GCATC 3 cut(s) 175, 592, 781
BpmI CTGGAG 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 737
BpuEI CTTGAG 1 cut(s) 68
BsaJI CCNNGG 2 cut(s) 236, 501
BseBI CCWGG 1 cut(s) 383
BseDI CCNNGG 2 cut(s) 236, 501
BseGI GGATG 3 cut(s) 58, 67, 572
BseMII CTCAG 1 cut(s) 135
BseRI GAGGAG 1 cut(s) 217
BseXI GCAGC 3 cut(s) 237, 331, 621
BshFI GGCC 2 cut(s) 234, 476
BshNI GGYRCC 1 cut(s) 742
BsiHKCI CYCGRG 1 cut(s) 684
BsmAI GTCTC 2 cut(s) 269, 681
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 2 cut(s) 234, 476
BsoBI CYCGRG 1 cut(s) 684
Bsp143I GATC 2 cut(s) 355, 486
Bsp19I CCATGG 1 cut(s) 236
BspACI CCGC 3 cut(s) 228, 231, 424
BspANI GGCC 2 cut(s) 234, 476
BspCNI CTCAG 1 cut(s) 134
BspHI TCATGA 2 cut(s) 489, 727
BspLI GGNNCC 2 cut(s) 744, 780
BspMI ACCTGC 1 cut(s) 774
BspT107I GGYRCC 1 cut(s) 742
BssECI CCNNGG 2 cut(s) 236, 501
BssMI GATC 2 cut(s) 355, 486
BssNAI GTATAC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 2 cut(s) 236, 501
Bst1107I GTATAC 1 cut(s) 316
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 383
Bst4CI ACNGT 1 cut(s) 149
Bst6I CTCTTC 1 cut(s) 194
BstDEI CTNAG 2 cut(s) 121, 737
BstDSI CCRYGG 1 cut(s) 236
BstF5I GGATG 3 cut(s) 58, 67, 572
BstKTI GATC 2 cut(s) 358, 489
BstMAI GTCTC 2 cut(s) 269, 681
BstMBI GATC 2 cut(s) 355, 486
BstMWI GCNNNNNNNGC 6 cut(s) 100, 180, 231, 383, 523, 611
BstNI CCWGG 1 cut(s) 383
BstNSI RCATGY 3 cut(s) 282, 390, 641
BstSCI CCNGG 1 cut(s) 381
BstV1I GCAGC 3 cut(s) 237, 331, 621
BstXI CCANNNNNNTGG 1 cut(s) 389
BstZ17I GTATAC 1 cut(s) 316
BsuRI GGCC 2 cut(s) 234, 476
BtgI CCRYGG 1 cut(s) 236
BtsCI GGATG 3 cut(s) 58, 67, 572
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 2 cut(s) 154, 318
BveI ACCTGC 1 cut(s) 774
CciI TCATGA 2 cut(s) 489, 727
Cfr13I GGNCC 1 cut(s) 233
Csp6I GTAC 3 cut(s) 125, 581, 743
CviAII CATG 7 cut(s) 104, 237, 279, 387, 490, 638, 728
CviJI RGCY 8 cut(s) 94, 120, 234, 469, 476, 500, 614, 736
CviKI_1 RGCY 8 cut(s) 94, 120, 234, 469, 476, 500, 614, 736
CviQI GTAC 3 cut(s) 125, 581, 743
DdeI CTNAG 2 cut(s) 121, 737
DpnI GATC 2 cut(s) 357, 488
DpnII GATC 2 cut(s) 355, 486
DriI GACNNNNNGTC 1 cut(s) 694
Eam1104I CTCTTC 1 cut(s) 194
Eam1105I GACNNNNNGTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 194
Eco130I CCWWGG 2 cut(s) 236, 501
Eco147I AGGCCT 1 cut(s) 476
Eco57I CTGAAG 1 cut(s) 693
Eco88I CYCGRG 1 cut(s) 684
EcoRII CCWGG 1 cut(s) 381
EcoT14I CCWWGG 2 cut(s) 236, 501
ErhI CCWWGG 2 cut(s) 236, 501
FaeI CATG 7 cut(s) 107, 240, 282, 390, 493, 641, 731
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FatI CATG 7 cut(s) 103, 236, 278, 386, 489, 637, 727
FauNDI CATATG 1 cut(s) 754
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 5 cut(s) 226, 229, 232, 345, 635
FokI GGATG 3 cut(s) 65, 74, 559
Fsp4HI GCNGC 5 cut(s) 226, 229, 232, 345, 635
GluI GCNGC 5 cut(s) 226, 229, 232, 345, 635
GsuI CTGGAG 1 cut(s) 594
HaeIII GGCC 2 cut(s) 234, 476
Hin1II CATG 7 cut(s) 107, 240, 282, 390, 493, 641, 731
HinfI GANTC 4 cut(s) 26, 107, 607, 682
HphI GGTGA 2 cut(s) 68, 382
Hpy166II GTNNAC 2 cut(s) 316, 713
Hpy188I TCNGA 7 cut(s) 171, 274, 355, 399, 598, 646, 681
Hpy188III TCNNGA 3 cut(s) 490, 686, 728
Hpy8I GTNNAC 2 cut(s) 316, 713
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4IV ACGT 1 cut(s) 418
HpyCH4V TGCA 4 cut(s) 347, 377, 637, 794
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 180, 231, 383, 523, 611
HpyF3I CTNAG 2 cut(s) 121, 737
HpySE526I ACGT 1 cut(s) 418
Hsp92II CATG 7 cut(s) 107, 240, 282, 390, 493, 641, 731
KpnI GGTACC 1 cut(s) 746
Kzo9I GATC 2 cut(s) 355, 486
LmnI GCTCC 3 cut(s) 91, 100, 152
Lsp1109I GCAGC 3 cut(s) 237, 331, 621
LweI GCATC 3 cut(s) 175, 592, 781
MaeII ACGT 1 cut(s) 418
MalI GATC 2 cut(s) 357, 488
MboI GATC 2 cut(s) 355, 486
MboII GAAGA 6 cut(s) 23, 95, 208, 211, 505, 578
MluCI AATT 5 cut(s) 286, 506, 518, 617, 795
MlyI GAGTC 2 cut(s) 601, 676
MnlI CCTC 6 cut(s) 43, 52, 186, 195, 361, 386
MroXI GAANNNNTTC 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 383
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 1 cut(s) 383
MwoI GCNNNNNNNGC 6 cut(s) 100, 180, 231, 383, 523, 611
NcoI CCATGG 1 cut(s) 236
NdeI CATATG 1 cut(s) 754
NdeII GATC 2 cut(s) 355, 486
NlaIII CATG 7 cut(s) 107, 240, 282, 390, 493, 641, 731
NlaIV GGNNCC 2 cut(s) 744, 780
NspI RCATGY 3 cut(s) 282, 390, 641
PaeR7I CTCGAG 1 cut(s) 684
PagI TCATGA 2 cut(s) 489, 727
PceI AGGCCT 1 cut(s) 476
PciI ACATGT 1 cut(s) 278
PctI GAATGC 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 26, 107
PkrI GCNGC 5 cut(s) 227, 230, 233, 346, 636
PleI GAGTC 2 cut(s) 601, 676
PpsI GAGTC 2 cut(s) 601, 676
PscI ACATGT 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 381
PspGI CCWGG 1 cut(s) 381
PspN4I GGNNCC 2 cut(s) 744, 780
PspPI GGNCC 1 cut(s) 233
RsaI GTAC 3 cut(s) 126, 582, 744
RsaNI GTAC 3 cut(s) 125, 581, 743
SatI GCNGC 5 cut(s) 226, 229, 232, 345, 635
Sau3AI GATC 2 cut(s) 355, 486
Sau96I GGNCC 1 cut(s) 233
ScaI AGTACT 1 cut(s) 582
SchI GAGTC 2 cut(s) 601, 676
ScrFI CCNGG 1 cut(s) 383
SetI ASST 9 cut(s) 48, 96, 266, 372, 397, 421, 433, 748, 768
SfaNI GCATC 3 cut(s) 175, 592, 781
Sfr274I CTCGAG 1 cut(s) 684
SlaI CTCGAG 1 cut(s) 684
SmlI CTYRAG 2 cut(s) 47, 684
SmoI CTYRAG 2 cut(s) 47, 684
Sse9I AATT 5 cut(s) 286, 506, 518, 617, 795
SseBI AGGCCT 1 cut(s) 476
SsiI CCGC 3 cut(s) 228, 231, 424
StuI AGGCCT 1 cut(s) 476
StyD4I CCNGG 1 cut(s) 381
StyI CCWWGG 2 cut(s) 236, 501
TaaI ACNGT 1 cut(s) 149
TaiI ACGT 1 cut(s) 421
TaqI TCGA 3 cut(s) 75, 81, 685
TaqII GACCGA 1 cut(s) 677
TasI AATT 5 cut(s) 286, 506, 518, 617, 795
TatI WGTACW 2 cut(s) 124, 580
TauI GCSGC 2 cut(s) 231, 234
TfiI GAWTC 2 cut(s) 26, 107
TscAI CASTG 2 cut(s) 154, 325
TseI GCWGC 3 cut(s) 225, 344, 634
TspDTI ATGAA 5 cut(s) 120, 506, 582, 716, 741
TspRI CASTG 2 cut(s) 154, 325
XapI RAATTY 3 cut(s) 286, 506, 617
XceI RCATGY 3 cut(s) 282, 390, 641
XhoI CTCGAG 1 cut(s) 684
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 1 cut(s) 518
ZrmI AGTACT 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.