RLG00000015866

PHAX RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008081
Physical Location & Seq
Reverse (-)
1983308 .. 1984829
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015866

Sequence Viewer

Length: 459 bp
ATGGAAGGAGAAGATAGCATTTTAGATTCAATATATGATGATAACTTTGAGGATGGTGAGGATGCCGAAATGCTCGATGTCGAGCACAAATCACAAAATGGCTCGGTACAGGGCAGTGGTGGAGCAAACCAAGAACATCAGAGCAAGGGTAGCAAGAATAAGAGACACAACAGCGGCGGCCCATGGACTCATATTTTTGGCATAGACAGGTTTGTTCGGGAGACATATAGAAATTTGAGAGAGCAGAAATCGTATATGGTATACATGAGGTTGGTTGTCTTGTTGTTGCTGCACTGGGTGAGCAACGCAATACAGGCATGTGGAGATCAGATGACTGCTAATGGCAGACTTATTTTGCGAACAGGTGGTGGCCTACTGTGGAATATCATCAAAGCACGAGAACCAAAGGCCTATAAAGAGATCATGAAGAAAGCAACGGAATTTGCGGTGCAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RNA_GG_bind

Protein Analysis

153

Amino Acids

17.28

Weight (kDa)

6.2

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHAX_RNA-bd PF10258 72 - 146 4e-09 Phosphorylated adapter RNA export protein, RNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 261
AciI CCGC 3 cut(s) 174, 177, 446
AcsI RAATTY 2 cut(s) 232, 440
AdeI CACNNNGTG 1 cut(s) 298
AfaI GTAC 1 cut(s) 108
AgsI TTSAA 1 cut(s) 30
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 2 cut(s) 157, 215
AoxI GGCC 3 cut(s) 178, 370, 408
ApeKI GCWGC 1 cut(s) 289
ApoI RAATTY 2 cut(s) 232, 440
AspS9I GGNCC 1 cut(s) 179
AsuHPI GGTGA 2 cut(s) 68, 310
BauI CACGAG 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 1 cut(s) 276
BccI CCATC 1 cut(s) 47
BcoDI GTCTC 2 cut(s) 157, 215
BisI GCNGC 3 cut(s) 175, 178, 290
BlsI GCNGC 3 cut(s) 176, 179, 291
BmgT120I GGNCC 1 cut(s) 179
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 1 cut(s) 52
BmuI ACTGGG 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 299
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 2 cut(s) 58, 67
BseNI ACTGG 1 cut(s) 299
BseXI GCAGC 1 cut(s) 276
BsgI GTGCAG 1 cut(s) 275
BshFI GGCC 3 cut(s) 180, 372, 410
BsiHKAI GWGCWC 1 cut(s) 87
BsmAI GTCTC 2 cut(s) 157, 215
BsnI GGCC 3 cut(s) 180, 372, 410
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 2 cut(s) 325, 420
Bsp19I CCATGG 1 cut(s) 182
BspACI CCGC 3 cut(s) 174, 177, 446
BspANI GGCC 3 cut(s) 180, 372, 410
BspHI TCATGA 1 cut(s) 423
BsrI ACTGG 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 2 cut(s) 325, 420
BssNAI GTATAC 1 cut(s) 262
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 182
Bst1107I GTATAC 1 cut(s) 262
Bst2BI CACGAG 1 cut(s) 396
Bst4CI ACNGT 1 cut(s) 378
BstDSI CCRYGG 1 cut(s) 182
BstF5I GGATG 2 cut(s) 58, 67
BstKTI GATC 2 cut(s) 328, 423
BstMAI GTCTC 2 cut(s) 157, 215
BstMBI GATC 2 cut(s) 325, 420
BstMWI GCNNNNNNNGC 2 cut(s) 150, 314
BstNSI RCATGY 1 cut(s) 321
BstV1I GCAGC 1 cut(s) 276
BstZ17I GTATAC 1 cut(s) 262
BsuRI GGCC 3 cut(s) 180, 372, 410
BtgI CCRYGG 1 cut(s) 182
BtsCI GGATG 2 cut(s) 58, 67
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 2 cut(s) 121, 292
CciI TCATGA 1 cut(s) 423
Cfr13I GGNCC 1 cut(s) 179
Csp6I GTAC 1 cut(s) 107
CviAII CATG 4 cut(s) 183, 265, 318, 424
CviJI RGCY 4 cut(s) 102, 180, 372, 410
CviKI_1 RGCY 4 cut(s) 102, 180, 372, 410
CviQI GTAC 1 cut(s) 107
DpnI GATC 2 cut(s) 327, 422
DpnII GATC 2 cut(s) 325, 420
DraIII CACNNNGTG 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 182
Eco147I AGGCCT 1 cut(s) 410
EcoT14I CCWWGG 1 cut(s) 182
ErhI CCWWGG 1 cut(s) 182
FaeI CATG 4 cut(s) 186, 268, 321, 427
FatI CATG 4 cut(s) 182, 264, 317, 423
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 3 cut(s) 175, 178, 290
FokI GGATG 2 cut(s) 65, 74
Fsp4HI GCNGC 3 cut(s) 175, 178, 290
GluI GCNGC 3 cut(s) 175, 178, 290
HaeIII GGCC 3 cut(s) 180, 372, 410
Hin1II CATG 4 cut(s) 186, 268, 321, 427
HinfI GANTC 2 cut(s) 26, 187
HphI GGTGA 2 cut(s) 68, 310
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 2 cut(s) 141, 330
Hpy188III TCNNGA 2 cut(s) 218, 424
Hpy8I GTNNAC 1 cut(s) 262
HpyCH4III ACNGT 1 cut(s) 378
HpyCH4V TGCA 2 cut(s) 292, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 150, 314
Hsp92II CATG 4 cut(s) 186, 268, 321, 427
Kzo9I GATC 2 cut(s) 325, 420
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 5 cut(s) 95, 193, 280, 299, 348
Lsp1109I GCAGC 1 cut(s) 276
LweI GCATC 1 cut(s) 52
MalI GATC 2 cut(s) 327, 422
MboI GATC 2 cut(s) 325, 420
MboII GAAGA 2 cut(s) 23, 439
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 2 cut(s) 232, 440
MlyI GAGTC 1 cut(s) 181
MnlI CCTC 3 cut(s) 43, 52, 261
MspA1I CMGCKG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 150, 314
NcoI CCATGG 1 cut(s) 182
NdeII GATC 2 cut(s) 325, 420
NlaIII CATG 4 cut(s) 186, 268, 321, 427
NspI RCATGY 1 cut(s) 321
PagI TCATGA 1 cut(s) 423
PceI AGGCCT 1 cut(s) 410
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 3 cut(s) 176, 179, 291
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PspPI GGNCC 1 cut(s) 179
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SatI GCNGC 3 cut(s) 175, 178, 290
Sau3AI GATC 2 cut(s) 325, 420
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 1 cut(s) 181
SduI GDGCHC 1 cut(s) 87
SetI ASST 3 cut(s) 212, 272, 367
SfaNI GCATC 1 cut(s) 52
Sse9I AATT 2 cut(s) 232, 440
SseBI AGGCCT 1 cut(s) 410
SsiI CCGC 3 cut(s) 174, 177, 446
StuI AGGCCT 1 cut(s) 410
StyI CCWWGG 1 cut(s) 182
TaaI ACNGT 1 cut(s) 378
TaqI TCGA 2 cut(s) 75, 81
TasI AATT 2 cut(s) 232, 440
TauI GCSGC 2 cut(s) 177, 180
TfiI GAWTC 1 cut(s) 26
TscAI CASTG 2 cut(s) 121, 299
TseI GCWGC 1 cut(s) 289
TspDTI ATGAA 1 cut(s) 440
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 2 cut(s) 121, 299
XapI RAATTY 2 cut(s) 232, 440
XceI RCATGY 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.