pycom15g06930

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009606
Physical Location & Seq
Reverse (-)
4234236 .. 4235654
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1164 bp
ATGCAAACTCACACCCCTATTGAAACTCTCTCACTTCCCATTTCAGTCTCTCACTGGTCAAGCTTTTTCCCCTTTTTTTTCAATTGCCCCCCAATTAAAAATCTTCCTCTCTCCCCCATCGCAATTTCAGAATCCTCCACTCTCTCAAAAAACCTCCGAAAGTTCGAAGCTTTTTTTCTCTCTCAAGACTCCAGAGTCGGAATTTCATCCCTGTTGAGGCGGATAATGGAAGGTTTTCGGTGCTTCCCGGAAACGAAGTCGCTTTGGGCTCCGGAATTTTCGTCGCCGGAATGTGTGCCCGGAAGCTCATCGTCGCAGAACCACATTGGAGGAGAATCCCATTCTGATCAAACCCTGGAGGCTGCCTTTTCTCGCCTAAATGTGTCAGCTTTCAGTCGCCAGCAGCCGCGGATTTCTGGCATTGACGGCGGCGATCACAGTAATGGGTCTGCGCTGAACTACCCATCAAGCGATCACGGATATAGCGGCGGAAATTCGTTGCCTTTTGGCGGCGGAGAGTCGTTACAGAGTGCTCTGAGCTTGCAGAAGGGCGGGGATGGGGTTTTTGGGAACCTTGGTCTTGAAAATTGCTGGGTGGACTCTGATATTACTAATGGCTCGAATGGCTTTGTTCCCAATCAAGATTGCTGGGTGCATTCTGACATTGCTGGGTTTCGTGAAACACTGGATCGGTTGTCGTTGGCTGATTTGCGCGGAAAAATTGCTGCTTTGGCCAAGGAGCAGTACGGCTGTCGTTTTCTGCAGAAAACAATGGATGTTGCACCGAACAGGGATGTGATTGATTTGATATTCTTGGAGGTTATGGAAAATGTGGTCGTTTTGATGCTTGACCCTCACGGGAACTATGTCGTCCAGAAGCTTGTGGAGGTTTGCAATGAAGAGCAGAGGACCCGTATGCTTTCGAAGTTGATAATGGAGAGTCATACGTCACTGGTTTGTATTGCCCTGGACAATCGCGGGACTCGCACTGTACAGAAATTGTTGCGGTATGTTACTAACCAAGAGCAAGTGTCGCTGATTATGGGAGCTCTGAGCCCTGGTGCCGCTGTGTTGAGTAAGAACAACAACGGCCAACATGTGATCGAATATTGCTTAGAGAATTTTTCTGAGGAAGACAACAGGGTTGCACGAATCCATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

388

Amino Acids

42.62

Weight (kDa)

5.49

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 277 - 311 1.8e-11 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 1061
AccII CGCG 3 cut(s) 409, 714, 978
AccIII TCCGGA 1 cut(s) 271
AclWI GGATC 1 cut(s) 696
AcoI YGGCCR 2 cut(s) 732, 1090
AcsI RAATTY 4 cut(s) 201, 275, 493, 1120
AfaI GTAC 2 cut(s) 746, 993
AfiI CCNNNNNNNGG 2 cut(s) 216, 509
AflIII ACRYGT 1 cut(s) 1096
AgsI TTSAA 3 cut(s) 23, 82, 584
AjnI CCWGG 3 cut(s) 354, 966, 1057
AluBI AGCT 7 cut(s) 63, 170, 306, 389, 540, 880, 1049
AluI AGCT 7 cut(s) 63, 170, 306, 389, 540, 880, 1049
Alw21I GWGCWC 2 cut(s) 535, 1051
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 1 cut(s) 696
Aor13HI TCCGGA 1 cut(s) 271
AoxI GGCC 2 cut(s) 732, 1090
ApeKI GCWGC 3 cut(s) 362, 403, 725
ApoI RAATTY 4 cut(s) 201, 275, 493, 1120
Asp700I GAANNNNTTC 1 cut(s) 234
AspLEI GCGC 2 cut(s) 454, 714
AspS9I GGNCC 1 cut(s) 909
AsuC2I CCSGG 2 cut(s) 248, 300
AsuII TTCGAA 2 cut(s) 165, 923
AvaII GGWCC 1 cut(s) 909
BaeGI GKGCMC 1 cut(s) 300
BalI TGGCCA 1 cut(s) 734
BanI GGYRCC 1 cut(s) 1061
BanII GRGCYC 3 cut(s) 271, 1051, 1058
BbsI GAAGAC 1 cut(s) 1140
Bbv12I GWGCWC 2 cut(s) 535, 1051
BbvI GCAGC 3 cut(s) 349, 415, 712
BccI CCATC 3 cut(s) 125, 472, 551
BceAI ACGGC 3 cut(s) 442, 763, 1105
BciT130I CCWGG 3 cut(s) 356, 968, 1059
BclI TGATCA 1 cut(s) 346
BcnI CCSGG 2 cut(s) 248, 300
BcoDI GTCTC 1 cut(s) 52
BfmI CTRYAG 1 cut(s) 761
BisI GCNGC 8 cut(s) 363, 404, 407, 430, 487, 511, 726, 1065
BlsI GCNGC 8 cut(s) 364, 405, 408, 431, 488, 512, 727, 1066
Bme1390I CCNGG 5 cut(s) 248, 300, 356, 968, 1059
Bme18I GGWCC 1 cut(s) 909
BmgT120I GGNCC 1 cut(s) 909
BmiI GGNNCC 4 cut(s) 270, 572, 911, 1063
BmrFI CCNGG 5 cut(s) 248, 300, 356, 968, 1059
BmsI GCATC 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 1140
BpmI CTGGAG 2 cut(s) 175, 377
Bpu14I TTCGAA 2 cut(s) 165, 923
BpuEI CTTGAG 1 cut(s) 168
BpuMI CCSGG 2 cut(s) 248, 300
BsaJI CCNNGG 6 cut(s) 354, 407, 574, 735, 966, 1057
BsaWI WCCGGW 1 cut(s) 271
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 509
Bse1I ACTGG 3 cut(s) 59, 690, 957
Bse3DI GCAATG 2 cut(s) 663, 901
BseAI TCCGGA 1 cut(s) 271
BseBI CCWGG 3 cut(s) 356, 968, 1059
BseDI CCNNGG 6 cut(s) 354, 407, 574, 735, 966, 1057
BseGI GGATG 4 cut(s) 206, 562, 781, 799
BseLI CCNNNNNNNGG 2 cut(s) 216, 509
BseMI GCAATG 2 cut(s) 663, 901
BseMII CTCAG 3 cut(s) 527, 1043, 1119
BseNI ACTGG 3 cut(s) 59, 690, 957
BseRI GAGGAG 1 cut(s) 345
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 3 cut(s) 349, 415, 712
BseYI CCCAGC 3 cut(s) 591, 648, 668
Bsh1236I CGCG 3 cut(s) 409, 714, 978
BshFI GGCC 2 cut(s) 734, 1092
BshNI GGYRCC 1 cut(s) 1061
BsiHKAI GWGCWC 2 cut(s) 535, 1051
BsiSI CCGG 4 cut(s) 248, 272, 287, 300
BslFI GGGAC 1 cut(s) 994
BslI CCNNNNNNNGG 2 cut(s) 216, 509
BsmAI GTCTC 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 994
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 2 cut(s) 734, 1092
Bsp119I TTCGAA 2 cut(s) 165, 923
Bsp1286I GDGCHC 5 cut(s) 271, 300, 535, 1051, 1058
Bsp13I TCCGGA 1 cut(s) 271
Bsp1407I TGTACA 1 cut(s) 991
Bsp143I GATC 5 cut(s) 346, 433, 472, 688, 1101
BspANI GGCC 2 cut(s) 734, 1092
BspCNI CTCAG 3 cut(s) 528, 1044, 1120
BspEI TCCGGA 1 cut(s) 271
BspFNI CGCG 3 cut(s) 409, 714, 978
BspLI GGNNCC 4 cut(s) 270, 572, 911, 1063
BspMAI CTGCAG 1 cut(s) 765
BspPI GGATC 1 cut(s) 696
BspQI GCTCTTC 1 cut(s) 894
BspT104I TTCGAA 2 cut(s) 165, 923
BspT107I GGYRCC 1 cut(s) 1061
BsrDI GCAATG 2 cut(s) 663, 901
BsrGI TGTACA 1 cut(s) 991
BsrI ACTGG 3 cut(s) 59, 690, 957
BssECI CCNNGG 6 cut(s) 354, 407, 574, 735, 966, 1057
BssMI GATC 5 cut(s) 346, 433, 472, 688, 1101
BssT1I CCWWGG 2 cut(s) 574, 735
Bst2UI CCWGG 3 cut(s) 356, 968, 1059
Bst4CI ACNGT 2 cut(s) 440, 991
Bst6I CTCTTC 1 cut(s) 894
BstAUI TGTACA 1 cut(s) 991
BstBI TTCGAA 2 cut(s) 165, 923
BstC8I GCNNGC 2 cut(s) 401, 542
BstDEI CTNAG 4 cut(s) 536, 1052, 1114, 1128
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 4 cut(s) 206, 562, 781, 799
BstFNI CGCG 3 cut(s) 409, 714, 978
BstHHI GCGC 2 cut(s) 454, 714
BstKTI GATC 5 cut(s) 349, 436, 475, 691, 1104
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 5 cut(s) 346, 433, 472, 688, 1101
BstMWI GCNNNNNNNGC 5 cut(s) 426, 624, 731, 984, 1033
BstNI CCWGG 3 cut(s) 356, 968, 1059
BstNSI RCATGY 1 cut(s) 1100
BstSCI CCNGG 5 cut(s) 246, 298, 354, 966, 1057
BstSFI CTRYAG 1 cut(s) 761
BstSLI GKGCMC 1 cut(s) 300
BstUI CGCG 3 cut(s) 409, 714, 978
BstV1I GCAGC 3 cut(s) 349, 415, 712
BstV2I GAAGAC 1 cut(s) 1140
BsuRI GGCC 2 cut(s) 734, 1092
BtgI CCRYGG 1 cut(s) 407
BtgZI GCGATG 1 cut(s) 103
BtsCI GGATG 4 cut(s) 206, 562, 781, 799
BtsIMutI CAGTG 4 cut(s) 52, 683, 950, 987
Cac8I GCNNGC 2 cut(s) 401, 542
CfoI GCGC 2 cut(s) 454, 714
Cfr13I GGNCC 1 cut(s) 909
Cfr42I CCGCGG 1 cut(s) 410
Csp6I GTAC 2 cut(s) 745, 992
CviAII CATG 2 cut(s) 1097, 1157
CviQI GTAC 2 cut(s) 745, 992
DdeI CTNAG 4 cut(s) 536, 1052, 1114, 1128
DpnI GATC 5 cut(s) 348, 435, 474, 690, 1103
DpnII GATC 5 cut(s) 346, 433, 472, 688, 1101
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
EaeI YGGCCR 2 cut(s) 732, 1090
Eam1104I CTCTTC 1 cut(s) 894
EarI CTCTTC 1 cut(s) 894
EciI GGCGGA 3 cut(s) 235, 504, 528
Ecl136II GAGCTC 1 cut(s) 1049
Eco130I CCWWGG 2 cut(s) 574, 735
Eco24I GRGCYC 3 cut(s) 271, 1051, 1058
Eco47I GGWCC 1 cut(s) 909
Eco53kI GAGCTC 1 cut(s) 1049
EcoICRI GAGCTC 1 cut(s) 1049
EcoO109I RGGNCCY 1 cut(s) 909
EcoRII CCWGG 3 cut(s) 354, 966, 1057
EcoT14I CCWWGG 2 cut(s) 574, 735
EcoT38I GRGCYC 3 cut(s) 271, 1051, 1058
ErhI CCWWGG 2 cut(s) 574, 735
FaeI CATG 2 cut(s) 1100, 1160
FaiI YATR 9 cut(s) 483, 824, 867, 917, 945, 1011, 1043, 1098, 1158
FaqI GGGAC 1 cut(s) 994
FatI CATG 2 cut(s) 1096, 1156
FauI CCCGC 2 cut(s) 545, 971
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 8 cut(s) 363, 404, 407, 430, 487, 511, 726, 1065
FokI GGATG 4 cut(s) 193, 569, 788, 806
FriOI GRGCYC 3 cut(s) 271, 1051, 1058
Fsp4HI GCNGC 8 cut(s) 363, 404, 407, 430, 487, 511, 726, 1065
GlaI GCGC 2 cut(s) 453, 713
GluI GCNGC 8 cut(s) 363, 404, 407, 430, 487, 511, 726, 1065
GsaI CCCAGC 3 cut(s) 595, 652, 672
GsuI CTGGAG 2 cut(s) 175, 377
HaeIII GGCC 2 cut(s) 734, 1092
HapII CCGG 4 cut(s) 248, 272, 287, 300
HhaI GCGC 2 cut(s) 454, 714
Hin1II CATG 2 cut(s) 1100, 1160
Hin6I GCGC 2 cut(s) 452, 712
HinP1I GCGC 2 cut(s) 452, 712
HindIII AAGCTT 3 cut(s) 61, 168, 878
HinfI GANTC 9 cut(s) 131, 188, 195, 335, 518, 599, 940, 982, 1152
HpaII CCGG 4 cut(s) 248, 272, 287, 300
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 9 cut(s) 130, 158, 200, 346, 537, 604, 661, 1053, 1129
Hpy188III TCNNGA 7 cut(s) 185, 192, 272, 581, 641, 677, 874
Hpy8I GTNNAC 1 cut(s) 598
Hpy99I CGWCG 2 cut(s) 286, 316
HpyAV CCTTC 2 cut(s) 224, 541
HpyCH4III ACNGT 2 cut(s) 440, 991
HpyCH4IV ACGT 1 cut(s) 947
HpyCH4V TGCA 7 cut(s) 4, 544, 655, 763, 782, 894, 1148
HpyF10VI GCNNNNNNNGC 5 cut(s) 426, 624, 731, 984, 1033
HpyF3I CTNAG 4 cut(s) 536, 1052, 1114, 1128
HpySE526I ACGT 1 cut(s) 947
Hsp92II CATG 2 cut(s) 1100, 1160
HspAI GCGC 2 cut(s) 452, 712
Kpn2I TCCGGA 1 cut(s) 271
Ksp22I TGATCA 1 cut(s) 346
KspI CCGCGG 1 cut(s) 410
Kzo9I GATC 5 cut(s) 346, 433, 472, 688, 1101
LguI GCTCTTC 1 cut(s) 894
LmnI GCTCC 3 cut(s) 274, 739, 1046
Lsp1109I GCAGC 3 cut(s) 349, 415, 712
LweI GCATC 1 cut(s) 834
MaeII ACGT 1 cut(s) 947
MaeIII GTNAC 3 cut(s) 522, 948, 1012
MalI GATC 5 cut(s) 348, 435, 474, 690, 1103
MboI GATC 5 cut(s) 346, 433, 472, 688, 1101
MboII GAAGA 3 cut(s) 95, 911, 1145
MfeI CAATTG 1 cut(s) 82
MhlI GDGCHC 5 cut(s) 271, 300, 535, 1051, 1058
MlsI TGGCCA 1 cut(s) 734
MluNI TGGCCA 1 cut(s) 734
MlyI GAGTC 6 cut(s) 182, 204, 527, 593, 949, 976
MmeI TCCRAC 1 cut(s) 178
Mox20I TGGCCA 1 cut(s) 734
MroI TCCGGA 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 234
MscI TGGCCA 1 cut(s) 734
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 441
Msp20I TGGCCA 1 cut(s) 734
MspA1I CMGCKG 2 cut(s) 409, 1067
MspI CCGG 4 cut(s) 248, 272, 287, 300
MspR9I CCNGG 5 cut(s) 248, 300, 356, 968, 1059
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 3 cut(s) 356, 968, 1059
MvnI CGCG 3 cut(s) 409, 714, 978
MwoI GCNNNNNNNGC 5 cut(s) 426, 624, 731, 984, 1033
NciI CCSGG 2 cut(s) 248, 300
NdeII GATC 5 cut(s) 346, 433, 472, 688, 1101
NlaIII CATG 2 cut(s) 1100, 1160
NlaIV GGNNCC 4 cut(s) 270, 572, 911, 1063
NmuCI GTSAC 1 cut(s) 948
NspI RCATGY 1 cut(s) 1100
NspV TTCGAA 2 cut(s) 165, 923
PciI ACATGT 1 cut(s) 1096
PciSI GCTCTTC 1 cut(s) 894
PctI GAATGC 1 cut(s) 655
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 3 cut(s) 131, 335, 1152
PfoI TCCNGGA 1 cut(s) 246
PkrI GCNGC 8 cut(s) 364, 405, 408, 431, 488, 512, 727, 1066
PleI GAGTC 6 cut(s) 182, 203, 526, 593, 948, 976
PpsI GAGTC 6 cut(s) 182, 203, 526, 593, 948, 976
PpuMI RGGWCCY 1 cut(s) 909
PscI ACATGT 1 cut(s) 1096
Psp124BI GAGCTC 1 cut(s) 1051
Psp5II RGGWCCY 1 cut(s) 909
Psp6I CCWGG 3 cut(s) 354, 966, 1057
PspFI CCCAGC 3 cut(s) 591, 648, 668
PspGI CCWGG 3 cut(s) 354, 966, 1057
PspN4I GGNNCC 4 cut(s) 270, 572, 911, 1063
PspPI GGNCC 1 cut(s) 909
PspPPI RGGWCCY 1 cut(s) 909
PstI CTGCAG 1 cut(s) 765
RsaI GTAC 2 cut(s) 746, 993
RsaNI GTAC 2 cut(s) 745, 992
RseI CAYNNNNRTG 1 cut(s) 441
SacI GAGCTC 1 cut(s) 1051
SacII CCGCGG 1 cut(s) 410
SapI GCTCTTC 1 cut(s) 894
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 8 cut(s) 363, 404, 407, 430, 487, 511, 726, 1065
Sau3AI GATC 5 cut(s) 346, 433, 472, 688, 1101
Sau96I GGNCC 1 cut(s) 909
SchI GAGTC 6 cut(s) 182, 204, 527, 593, 949, 976
ScrFI CCNGG 5 cut(s) 248, 300, 356, 968, 1059
SduI GDGCHC 5 cut(s) 271, 300, 535, 1051, 1058
SfaNI GCATC 1 cut(s) 834
SfcI CTRYAG 1 cut(s) 761
Sfr303I CCGCGG 1 cut(s) 410
SfuI TTCGAA 2 cut(s) 165, 923
SgrBI CCGCGG 1 cut(s) 410
SinI GGWCC 1 cut(s) 909
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 183
SmoI CTYRAG 1 cut(s) 183
SspI AATATT 1 cut(s) 1109
SstI GAGCTC 1 cut(s) 1051
StyD4I CCNGG 5 cut(s) 246, 298, 354, 966, 1057
StyI CCWWGG 2 cut(s) 574, 735
TaaI ACNGT 2 cut(s) 440, 991
TaiI ACGT 1 cut(s) 950
TaqI TCGA 4 cut(s) 165, 620, 923, 1104
TatI WGTACW 1 cut(s) 991
TauI GCSGC 5 cut(s) 409, 432, 489, 513, 1067
TfiI GAWTC 3 cut(s) 131, 335, 1152
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 4 cut(s) 59, 690, 957, 994
TseFI GTSAC 1 cut(s) 948
TseI GCWGC 3 cut(s) 362, 403, 725
Tsp45I GTSAC 1 cut(s) 948
TspDTI ATGAA 2 cut(s) 195, 912
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 4 cut(s) 59, 690, 957, 994
VpaK11BI GGWCC 1 cut(s) 909
XapI RAATTY 4 cut(s) 201, 275, 493, 1120
XceI RCATGY 1 cut(s) 1100
XmnI GAANNNNTTC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.