pycom15g07150

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011720
Physical Location & Seq
Forward (+)
4373042 .. 4375295
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 909 bp
ATGCTGCTTTTCGTGTTGGTCTTGTCCCTTGTGGCTCAAGCCTGCTCAGCACCGACAACTGCCCAACAACCAAACCAAAAAACAGATCAACATGTCAAAAGCATATTCATCCTGGCCGGACAGAGCAACATGGCAGGTCGAGGTGGTGTGGTTAACGACACCACCACAGGCATCATCACCTGGGACGGTGTCGTGCCGCCAAAGTGCCGCCCCAACCCGTCAGTCTTCCGGCTCGACGCAAATCTCAGGTGGGTTAAAGCACGCGAGCCTCTCCACGCCGACATCGACGTAGGGAAGACCAATGGGATTGGACCCGGTATGGCTTTTGCCAATGCGGTTTTGGCCAATCACTCTAGTACGGTTGGCCGGATTGGGTTAGTCCCTTGTGCGATCGGTGGGACTAACATAACCCAGTGGGCACGAGGCTCTTTTCTGTACAACCAGATGATCGGGAGGGCCAAGGTTTCGTTGCAGAATGGTGGTTCGATCAGAGCACTTCTGTGGTATCAGGGCGAGAGCGATACAGCTCGTAGAGAAGACGCTCGGTCTTACAAAAACAGATTGGAAACTTTTTTGGGGGATGTACGTTTGGATCTGCAATCTCCTGATCTCCCAATAATCCAGGTGGCTCTAGCATCTGGGGAAGAAGCAAGTCTGATAGAGATAGTGAGAGAAGCCCAGCTGGGAGTCGACTTTCTAAACGTGAGAACCGTCGACGCGAAAGGTCTTCCGTTGGAACCTGATGGCTTACACCTCACCACCCCTGCCCAGGTTCAACTTGGGAAAATGTTGGCTGATGCATATCTTCAATTCCTGCCCAGCGCCATAAGTCATGCTCCAGTAACATATCCTAATTATATTTTCCATTTTCTACTTTATTTGGTATTGAAAGTTCTCACCACGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

303

Amino Acids

32.7

Weight (kDa)

8.42

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 33 - 270 4.2e-92 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53010
fragaria_vesca FvH4_2g33800
malus_domestica MD08G1092200.v1.1 MD15G1076700.v1.1
prunus_persica Prupe.1G430100_v2.0.a1
pyrus_communis pycom08g07490 pycom08g07500 pycom15g07150
rosa_chinensis RchiOBHm_Chr6g0308761
rosa_laevigata RLG00000010622
rosa_multiflora Rmu_sc0000871.1_g000025 Rmu_sc0009346.1_g000012
rosa_roxburghii Rroxscaffold_7G00159960
rosa_rugosa Rorug06G0368100
rosa_samantha Rh6AG479200 Rh6BG488800 Rh6CG494000 Rh6DG479600
rosa_wichuraiana Rw6G041770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 125
AccI GTMKAC 2 cut(s) 690, 714
AccII CGCG 2 cut(s) 264, 719
AciI CCGC 3 cut(s) 197, 208, 335
AclWI GGATC 1 cut(s) 600
AcoI YGGCCR 3 cut(s) 114, 342, 364
AfaI GTAC 3 cut(s) 358, 437, 585
AfiI CCNNNNNNNGG 1 cut(s) 769
AflIII ACRYGT 1 cut(s) 91
AgsI TTSAA 3 cut(s) 776, 809, 889
AhdI GACNNNNNGTC 1 cut(s) 544
AjnI CCWGG 4 cut(s) 111, 179, 621, 768
AleI CACNNNNGTG 1 cut(s) 499
AluBI AGCT 3 cut(s) 527, 682, 906
AluI AGCT 3 cut(s) 527, 682, 906
Alw21I GWGCWC 1 cut(s) 496
AlwI GGATC 1 cut(s) 600
AoxI GGCC 4 cut(s) 114, 342, 364, 456
ApeKI GCWGC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 824
AspS9I GGNCC 2 cut(s) 311, 456
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 3 cut(s) 169, 748, 889
AvaII GGWCC 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 421
BalI TGGCCA 1 cut(s) 344
BauI CACGAG 2 cut(s) 420, 901
BbsI GAAGAC 4 cut(s) 217, 302, 543, 719
Bbv12I GWGCWC 1 cut(s) 496
BccI CCATC 1 cut(s) 737
BciT130I CCWGG 4 cut(s) 113, 181, 623, 770
BcnI CCSGG 1 cut(s) 315
BfaI CTAG 3 cut(s) 354, 632, 907
BfoI RGCGCY 1 cut(s) 825
BfuAI ACCTGC 1 cut(s) 125
BisI GCNGC 3 cut(s) 5, 197, 208
BlpI GCTNAGC 1 cut(s) 46
BlsI GCNGC 3 cut(s) 6, 198, 209
Bme1390I CCNGG 5 cut(s) 113, 181, 315, 623, 770
Bme18I GGWCC 1 cut(s) 311
BmeRI GACNNNNNGTC 1 cut(s) 544
BmgT120I GGNCC 2 cut(s) 311, 456
BmiI GGNNCC 2 cut(s) 313, 738
BmrFI CCNGG 5 cut(s) 113, 181, 315, 623, 770
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 3 cut(s) 180, 644, 787
BmuI ACTGGG 1 cut(s) 406
BpiI GAAGAC 4 cut(s) 217, 302, 543, 719
BpmI CTGGAG 1 cut(s) 822
Bpu1102I GCTNAGC 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 801
BsaJI CCNNGG 3 cut(s) 180, 459, 768
Bsc4I CCNNNNNNNGG 1 cut(s) 769
Bse1I ACTGG 2 cut(s) 412, 839
Bse8I GATNNNNATC 1 cut(s) 801
BseBI CCWGG 4 cut(s) 113, 181, 623, 770
BseDI CCNNGG 3 cut(s) 180, 459, 768
BseGI GGATG 2 cut(s) 108, 586
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 1 cut(s) 769
BseMII CTCAG 2 cut(s) 60, 259
BseNI ACTGG 2 cut(s) 412, 839
BseSI GKGCMC 1 cut(s) 421
BseYI CCCAGC 3 cut(s) 678, 682, 818
Bsh1236I CGCG 2 cut(s) 264, 719
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 4 cut(s) 116, 344, 366, 458
BsiEI CGRYCG 1 cut(s) 393
BsiHKAI GWGCWC 1 cut(s) 496
BsiSI CCGG 4 cut(s) 117, 229, 315, 367
BslFI GGGAC 4 cut(s) 10, 197, 365, 412
BslI CCNNNNNNNGG 1 cut(s) 769
BsmFI GGGAC 4 cut(s) 10, 197, 365, 412
BsnI GGCC 4 cut(s) 116, 344, 366, 458
Bsp1286I GDGCHC 2 cut(s) 421, 496
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 6 cut(s) 85, 390, 447, 486, 592, 607
Bsp1720I GCTNAGC 1 cut(s) 46
BspACI CCGC 3 cut(s) 197, 208, 335
BspANI GGCC 4 cut(s) 116, 344, 366, 458
BspCNI CTCAG 2 cut(s) 59, 258
BspFNI CGCG 2 cut(s) 264, 719
BspLI GGNNCC 2 cut(s) 313, 738
BspMI ACCTGC 1 cut(s) 125
BspPI GGATC 1 cut(s) 600
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 2 cut(s) 412, 839
BssECI CCNNGG 3 cut(s) 180, 459, 768
BssMI GATC 6 cut(s) 85, 390, 447, 486, 592, 607
BssSI CACGAG 2 cut(s) 420, 901
BssT1I CCWWGG 1 cut(s) 459
Bst2BI CACGAG 2 cut(s) 420, 901
Bst2UI CCWGG 4 cut(s) 113, 181, 623, 770
Bst4CI ACNGT 3 cut(s) 188, 361, 712
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 43, 262, 266
BstDEI CTNAG 2 cut(s) 46, 245
BstF5I GGATG 2 cut(s) 108, 586
BstFNI CGCG 2 cut(s) 264, 719
BstH2I RGCGCY 1 cut(s) 825
BstHHI GCGC 1 cut(s) 824
BstKTI GATC 6 cut(s) 88, 393, 450, 489, 595, 610
BstMBI GATC 6 cut(s) 85, 390, 447, 486, 592, 607
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 2 cut(s) 47, 341
BstNI CCWGG 4 cut(s) 113, 181, 623, 770
BstNSI RCATGY 1 cut(s) 95
BstSCI CCNGG 5 cut(s) 111, 179, 313, 621, 768
BstSLI GKGCMC 1 cut(s) 421
BstUI CGCG 2 cut(s) 264, 719
BstV2I GAAGAC 4 cut(s) 217, 302, 543, 719
BstX2I RGATCY 1 cut(s) 592
BstYI RGATCY 1 cut(s) 592
BsuRI GGCC 4 cut(s) 116, 344, 366, 458
BtsCI GGATG 2 cut(s) 108, 586
BtsIMutI CAGTG 1 cut(s) 419
BveI ACCTGC 1 cut(s) 125
Cac8I GCNNGC 3 cut(s) 43, 262, 266
CfoI GCGC 1 cut(s) 824
Cfr13I GGNCC 2 cut(s) 311, 456
CseI GACGC 3 cut(s) 245, 548, 725
Csp6I GTAC 3 cut(s) 357, 436, 584
CviAII CATG 3 cut(s) 92, 130, 833
CviQI GTAC 3 cut(s) 357, 436, 584
DdeI CTNAG 2 cut(s) 46, 245
DpnI GATC 6 cut(s) 87, 392, 449, 488, 594, 609
DpnII GATC 6 cut(s) 85, 390, 447, 486, 592, 607
DriI GACNNNNNGTC 1 cut(s) 544
EaeI YGGCCR 3 cut(s) 114, 342, 364
Eam1105I GACNNNNNGTC 1 cut(s) 544
Eco130I CCWWGG 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 311
EcoRII CCWGG 4 cut(s) 111, 179, 621, 768
EcoT14I CCWWGG 1 cut(s) 459
EcoT22I ATGCAT 1 cut(s) 802
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 3 cut(s) 95, 133, 836
FaqI GGGAC 4 cut(s) 10, 197, 365, 412
FatI CATG 3 cut(s) 91, 129, 832
FblI GTMKAC 2 cut(s) 690, 714
Fnu4HI GCNGC 3 cut(s) 5, 197, 208
FokI GGATG 2 cut(s) 95, 593
Fsp4HI GCNGC 3 cut(s) 5, 197, 208
FspBI CTAG 3 cut(s) 354, 632, 907
GlaI GCGC 1 cut(s) 823
GluI GCNGC 3 cut(s) 5, 197, 208
GsaI CCCAGC 3 cut(s) 682, 686, 822
GsuI CTGGAG 1 cut(s) 822
HaeII RGCGCY 1 cut(s) 825
HaeIII GGCC 4 cut(s) 116, 344, 366, 458
HapII CCGG 4 cut(s) 117, 229, 315, 367
HgaI GACGC 3 cut(s) 245, 548, 725
HhaI GCGC 1 cut(s) 824
Hin1II CATG 3 cut(s) 95, 133, 836
Hin6I GCGC 1 cut(s) 822
HinP1I GCGC 1 cut(s) 822
HincII GTYRAC 3 cut(s) 154, 691, 715
HindII GTYRAC 3 cut(s) 154, 691, 715
HinfI GANTC 1 cut(s) 687
HpaI GTTAAC 1 cut(s) 154
HpaII CCGG 4 cut(s) 117, 229, 315, 367
HphI GGTGA 3 cut(s) 169, 748, 889
Hpy166II GTNNAC 3 cut(s) 154, 691, 715
Hpy188I TCNGA 2 cut(s) 491, 657
Hpy188III TCNNGA 2 cut(s) 451, 605
Hpy8I GTNNAC 3 cut(s) 154, 691, 715
Hpy99I CGWCG 4 cut(s) 239, 290, 716, 719
HpyCH4III ACNGT 3 cut(s) 188, 361, 712
HpyCH4IV ACGT 3 cut(s) 288, 586, 702
HpyCH4V TGCA 3 cut(s) 472, 598, 800
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 341
HpyF3I CTNAG 2 cut(s) 46, 245
HpySE526I ACGT 3 cut(s) 288, 586, 702
Hsp92II CATG 3 cut(s) 95, 133, 836
HspAI GCGC 1 cut(s) 822
KspAI GTTAAC 1 cut(s) 154
Kzo9I GATC 6 cut(s) 85, 390, 447, 486, 592, 607
LmnI GCTCC 1 cut(s) 841
LweI GCATC 3 cut(s) 180, 644, 787
MaeI CTAG 3 cut(s) 354, 632, 907
MaeII ACGT 3 cut(s) 288, 586, 702
MaeIII GTNAC 1 cut(s) 841
MalI GATC 6 cut(s) 87, 392, 449, 488, 594, 609
MboI GATC 6 cut(s) 85, 390, 447, 486, 592, 607
MboII GAAGA 6 cut(s) 217, 307, 548, 656, 719, 797
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 2 cut(s) 421, 496
MlsI TGGCCA 1 cut(s) 344
MluCI AATT 2 cut(s) 809, 853
MluNI TGGCCA 1 cut(s) 344
MlyI GAGTC 1 cut(s) 696
MmeI TCCRAC 1 cut(s) 714
MnlI CCTC 5 cut(s) 134, 279, 416, 447, 764
Mox20I TGGCCA 1 cut(s) 344
Mph1103I ATGCAT 1 cut(s) 802
MscI TGGCCA 1 cut(s) 344
MseI TTAA 2 cut(s) 153, 255
MslI CAYNNNNRTG 1 cut(s) 499
Msp20I TGGCCA 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 682
MspI CCGG 4 cut(s) 117, 229, 315, 367
MspR9I CCNGG 5 cut(s) 113, 181, 315, 623, 770
MvaI CCWGG 4 cut(s) 113, 181, 623, 770
MvnI CGCG 2 cut(s) 264, 719
MwoI GCNNNNNNNGC 2 cut(s) 47, 341
NciI CCSGG 1 cut(s) 315
NdeII GATC 6 cut(s) 85, 390, 447, 486, 592, 607
NlaIII CATG 3 cut(s) 95, 133, 836
NlaIV GGNNCC 2 cut(s) 313, 738
NsiI ATGCAT 1 cut(s) 802
NspI RCATGY 1 cut(s) 95
OliI CACNNNNGTG 1 cut(s) 499
PciI ACATGT 1 cut(s) 91
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 708
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 3 cut(s) 6, 198, 209
Ple19I CGATCG 1 cut(s) 393
PleI GAGTC 1 cut(s) 695
PpsI GAGTC 1 cut(s) 695
PscI ACATGT 1 cut(s) 91
Psp6I CCWGG 4 cut(s) 111, 179, 621, 768
PspFI CCCAGC 3 cut(s) 678, 682, 818
PspGI CCWGG 4 cut(s) 111, 179, 621, 768
PspN4I GGNNCC 2 cut(s) 313, 738
PspPI GGNCC 2 cut(s) 311, 456
PsrI GAACNNNNNNTAC 2 cut(s) 876, 908
PsuI RGATCY 1 cut(s) 592
PsyI GACNNNGTC 1 cut(s) 188
PvuI CGATCG 1 cut(s) 393
PvuII CAGCTG 1 cut(s) 682
RsaI GTAC 3 cut(s) 358, 437, 585
RsaNI GTAC 3 cut(s) 357, 436, 584
RseI CAYNNNNRTG 1 cut(s) 499
SalI GTCGAC 2 cut(s) 689, 713
SaqAI TTAA 2 cut(s) 153, 255
SatI GCNGC 3 cut(s) 5, 197, 208
Sau3AI GATC 6 cut(s) 85, 390, 447, 486, 592, 607
Sau96I GGNCC 2 cut(s) 311, 456
SchI GAGTC 1 cut(s) 696
ScrFI CCNGG 5 cut(s) 113, 181, 315, 623, 770
SduI GDGCHC 2 cut(s) 421, 496
SfaNI GCATC 3 cut(s) 180, 644, 787
SgrDI CGTCGACG 1 cut(s) 713
SinI GGWCC 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 499
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
Sse9I AATT 2 cut(s) 809, 853
SsiI CCGC 3 cut(s) 197, 208, 335
SspMI CTAG 3 cut(s) 354, 632, 907
StyD4I CCNGG 5 cut(s) 111, 179, 313, 621, 768
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 3 cut(s) 188, 361, 712
TaiI ACGT 3 cut(s) 291, 589, 705
TaqI TCGA 6 cut(s) 139, 234, 285, 485, 690, 714
TaqII GACCGA 1 cut(s) 534
TasI AATT 2 cut(s) 809, 853
TatI WGTACW 1 cut(s) 435
TauI GCSGC 2 cut(s) 199, 210
Tru1I TTAA 2 cut(s) 153, 255
Tru9I TTAA 2 cut(s) 153, 255
TscAI CASTG 1 cut(s) 419
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 97
TspGWI ACGGA 1 cut(s) 720
TspRI CASTG 1 cut(s) 419
Tth111I GACNNNGTC 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 311
XceI RCATGY 1 cut(s) 95
XcmI CCANNNNNNNNNTGG 2 cut(s) 337, 776
XmiI GTMKAC 2 cut(s) 690, 714
XspI CTAG 3 cut(s) 354, 632, 907
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.