Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0008219

Physical Location & Seq

Genomic Coordinates Reverse (-)

4982549 .. 4985759

Gene Structure 3211 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1083 bp

ATGTTTGTATTTCATCGTAGGCTCAGCCCACTAGTCATAAGCAAGGATTTTTCCGAATATTTATTTATAAACGAGAGCAGAGCTGTCTTGCTAGAAAAGAAAAGCAGAGAGCTCCCCAAACCCAACACACTGAAAGCGAAGAGAATGTCTGGCATGGAGAGGCTTCAGAGGATGTTCGCCGGCGCCGGAGGAGCTTTGGGCCACCCTCCTCCAGATTCTCCCACCCTAGATTCCTCCGAGCAGGTTTACATCTCCTCCCTTGCCCTCCTCAAGATGCTCAAGCACGGAAGGGCGGGAGTCCCCATGGAAGTGATGGGATTGATGCTCGGTGAGTTTGTGGATGAGTACACTGTTCGAGTTGTCGATGTCTTTGCGATGCCGCAGAGTGGTACTGGTGTTAGTGTCGAAGCTGTTGACCATGTTTTCCAGACTAACATGCTTGATATGCTGAAGCAGACTGGAAGACCGGAGATGGTGGTAGGGTGGTACCACTCGCATCCTGGATTTGGCTGTTGGCTATCTGGTGTGGACATTAATACACAGCAGAGCTTTGAAGCTTTGAATCAACGTGCTGTGGCTGTGGTGGTTGATCCCATCCAGAGCGTGAAGGGAAAGGTCGTCATAGATGCCTTCCGTCTCATTAACCCACAAACAATGATGCTCGGCCAAGAACCACGGCAGACAACTTCAAATCTTGGGCATCTTAATAAGCCATCCATTCAAGCATTGATCCATGGTTTGAACCGGCATTATTACTCTATAGCTATTAATTACAGAAAGAATGAGCTTGAGGAGAAAATGCTGCTTAACCTCCACAAGAAGAAATGGACAGATGGGCTGACACTTAGGCGATTTGATAATCATTCGAAGACAAATGAGCAAACTGTGGAGGAGATGAAGAACTTGGCTGTCAAATACAACAAAGCAGTTCAAGAGGAGGATGAGTTACCACCCGAGAAGCTTGCAATTGCCAATGTAGGAAGGCAGGATGCGAAGAAGCATCTCGAAGAACATGTCTCCAACCTGATGTCCTCCAACATCGTTCAGACCCTGGGAACAATGCTCGACACTGTCGTGTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009651 GO:0019538 GO:0030163 GO:0032991 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 ko05169 map03050 map05169

Pfam Domains

Protein Families

JAB MitMem_reg

Protein Analysis

361

Amino Acids

40.58

Weight (kDa)

8.44

Isoelectric Point (pI)

43.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
JAB	PF01398	78 - 189	1.2e-34	JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Prok-JAB	PF14464	89 - 178	2.2e-06	Prokaryotic homologs of the JAB domain
RPN11_C	PF23594	279 - 358	8.2e-34	26S proteasome regulatory subunit RPN11 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008219)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23540 AT5G23540
fragaria_vesca	FvH4_2g34740 FvH4_2g34740 FvH4_2g34740
malus_domestica	MD08G1104100.v1.1 MD15G1085300.v1.1
prunus_persica	Prupe.1G439400_v2.0.a1
pyrus_communis	pycom08g08550 pycom15g08050
rosa_chinensis	RchiOBHm_Chr6g0263451 RchiOBHm_Chr6g0307401
rosa_laevigata	RLG00000010717 RLG00000014338
rosa_multiflora	Rmu_sc0000031.1_g000014 Rmu_sc0005715.1_g000007
rosa_rugosa	Rorug06G0358400 Rorug06G0358500
rosa_samantha	Rh6BG430700 Rh6BG431500 Rh6CG484000 Rh6DG470800
rosa_wichuraiana	Rw6G040870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	68
Acc36I	ACCTGC	1 cut(s)	232
Acc65I	GGTACC	1 cut(s)	486
AccB1I	GGYRCC	2 cut(s)	182, 486
AciI	CCGC	2 cut(s)	293, 380
AclWI	GGATC	2 cut(s)	584, 724
AcoI	YGGCCR	1 cut(s)	664
AcuI	CTGAAG	2 cut(s)	149, 470
AcyI	GRCGYC	1 cut(s)	183
AfaI	GTAC	3 cut(s)	347, 391, 488
AfiI	CCNNNNNNNGG	2 cut(s)	386, 506
AflIII	ACRYGT	1 cut(s)	1012
AgsI	TTSAA	6 cut(s)	554, 562, 690, 722, 742, 932
AhlI	ACTAGT	1 cut(s)	31
AjnI	CCWGG	2 cut(s)	499, 1050
AleI	CACNNNNGTG	1 cut(s)	1073
AluBI	AGCT	9 cut(s)	83, 112, 194, 410, 549, 557, 764, 787, 961
AluI	AGCT	9 cut(s)	83, 112, 194, 410, 549, 557, 764, 787, 961
Alw21I	GWGCWC	1 cut(s)	114
Alw26I	GTCTC	2 cut(s)	641, 1021
AlwI	GGATC	2 cut(s)	584, 724
AlwNI	CAGNNNCTG	1 cut(s)	1051
Ama87I	CYCGRG	1 cut(s)	953
AoxI	GGCC	2 cut(s)	199, 664
ApeKI	GCWGC	1 cut(s)	802
ArsI	GACNNNNNNTTYG	4 cut(s)	131, 163, 999, 1031
AseI	ATTAAT	2 cut(s)	534, 768
Asp718I	GGTACC	1 cut(s)	486
AspLEI	GCGC	1 cut(s)	185
AspS9I	GGNCC	1 cut(s)	199
AsuHPI	GGTGA	1 cut(s)	341
AsuII	TTCGAA	1 cut(s)	866
AvaI	CYCGRG	1 cut(s)	953
BaeI	ACNNNNGTAYC	2 cut(s)	381, 414
BanI	GGYRCC	2 cut(s)	182, 486
BanII	GRGCYC	1 cut(s)	114
BbsI	GAAGAC	2 cut(s)	469, 875
Bbv12I	GWGCWC	1 cut(s)	114
BbvI	GCAGC	1 cut(s)	789
BccI	CCATC	5 cut(s)	307, 466, 602, 721, 827
BceAI	ACGGC	1 cut(s)	692
BcgI	CGANNNNNNTGC	4 cut(s)	353, 387, 944, 978
BciT130I	CCWGG	2 cut(s)	501, 1052
BcoDI	GTCTC	2 cut(s)	641, 1021
BcuI	ACTAGT	1 cut(s)	31
BfaI	CTAG	4 cut(s)	32, 92, 227, 1081
BfmI	CTRYAG	1 cut(s)	759
BfoI	RGCGCY	1 cut(s)	186
BfuAI	ACCTGC	1 cut(s)	232
BisI	GCNGC	2 cut(s)	380, 803
BlpI	GCTNAGC	1 cut(s)	23
BlsI	GCNGC	2 cut(s)	381, 804
Bme1390I	CCNGG	2 cut(s)	501, 1052
BmeT110I	CYCGRG	1 cut(s)	953
BmgT120I	GGNCC	1 cut(s)	199
BmiI	GGNNCC	2 cut(s)	184, 488
BmrFI	CCNGG	2 cut(s)	501, 1052
BmsI	GCATC	9 cut(s)	264, 312, 366, 505, 616, 648, 709, 979, 1009
BpiI	GAAGAC	2 cut(s)	469, 875
BpmI	CTGGAG	1 cut(s)	195
Bpu1102I	GCTNAGC	1 cut(s)	23
Bpu14I	TTCGAA	1 cut(s)	866
BpuEI	CTTGAG	3 cut(s)	254, 263, 809
BsaHI	GRCGYC	1 cut(s)	183
BsaJI	CCNNGG	5 cut(s)	303, 674, 733, 1050, 1051
BsaWI	WCCGGW	1 cut(s)	466
BsaXI	ACNNNNNCTCC	4 cut(s)	239, 269, 929, 959
Bsc4I	CCNNNNNNNGG	2 cut(s)	386, 506
Bse118I	RCCGGY	2 cut(s)	179, 744
Bse1I	ACTGG	2 cut(s)	397, 463
BseBI	CCWGG	2 cut(s)	501, 1052
BseDI	CCNNGG	5 cut(s)	303, 674, 733, 1050, 1051
BseGI	GGATG	7 cut(s)	177, 346, 496, 594, 713, 946, 994
BseLI	CCNNNNNNNGG	2 cut(s)	386, 506
BseMII	CTCAG	1 cut(s)	37
BseNI	ACTGG	2 cut(s)	397, 463
BseRI	GAGGAG	7 cut(s)	198, 204, 244, 257, 806, 905, 950
BseXI	GCAGC	1 cut(s)	789
BshFI	GGCC	2 cut(s)	201, 666
BshNI	GGYRCC	2 cut(s)	182, 486
BsiHKAI	GWGCWC	1 cut(s)	114
BsiHKCI	CYCGRG	1 cut(s)	953
BsiSI	CCGG	4 cut(s)	180, 186, 467, 745
BslFI	GGGAC	1 cut(s)	284
BslI	CCNNNNNNNGG	2 cut(s)	386, 506
BsmAI	GTCTC	2 cut(s)	641, 1021
BsmBI	CGTCTC	1 cut(s)	641
BsmFI	GGGAC	1 cut(s)	284
BsnI	GGCC	2 cut(s)	201, 666
BsoBI	CYCGRG	1 cut(s)	953
Bsp119I	TTCGAA	1 cut(s)	866
Bsp1286I	GDGCHC	1 cut(s)	114
Bsp143I	GATC	2 cut(s)	589, 729
Bsp1720I	GCTNAGC	1 cut(s)	23
Bsp19I	CCATGG	2 cut(s)	303, 733
BspACI	CCGC	2 cut(s)	293, 380
BspANI	GGCC	2 cut(s)	201, 666
BspCNI	CTCAG	1 cut(s)	36
BspLI	GGNNCC	2 cut(s)	184, 488
BspMI	ACCTGC	1 cut(s)	232
BspPI	GGATC	2 cut(s)	584, 724
BspT104I	TTCGAA	1 cut(s)	866
BspT107I	GGYRCC	2 cut(s)	182, 486
BsrFI	RCCGGY	2 cut(s)	179, 744
BsrI	ACTGG	2 cut(s)	397, 463
BssAI	RCCGGY	2 cut(s)	179, 744
BssECI	CCNNGG	5 cut(s)	303, 674, 733, 1050, 1051
BssMI	GATC	2 cut(s)	589, 729
BssNI	GRCGYC	1 cut(s)	183
BssT1I	CCWWGG	2 cut(s)	303, 733
Bst2UI	CCWGG	2 cut(s)	501, 1052
Bst4CI	ACNGT	3 cut(s)	352, 886, 1072
Bst6I	CTCTTC	1 cut(s)	134
BstACI	GRCGYC	1 cut(s)	183
BstBI	TTCGAA	1 cut(s)	866
BstC8I	GCNNGC	2 cut(s)	181, 963
BstDEI	CTNAG	2 cut(s)	23, 845
BstDSI	CCRYGG	3 cut(s)	303, 674, 733
BstF5I	GGATG	7 cut(s)	177, 346, 496, 594, 713, 946, 994
BstH2I	RGCGCY	1 cut(s)	186
BstHHI	GCGC	1 cut(s)	185
BstKTI	GATC	2 cut(s)	592, 732
BstMAI	GTCTC	2 cut(s)	641, 1021
BstMBI	GATC	2 cut(s)	589, 729
BstMWI	GCNNNNNNNGC	2 cut(s)	191, 445
BstNI	CCWGG	2 cut(s)	501, 1052
BstNSI	RCATGY	2 cut(s)	439, 1016
BstSCI	CCNGG	2 cut(s)	499, 1050
BstSFI	CTRYAG	1 cut(s)	759
BstV1I	GCAGC	1 cut(s)	789
BstV2I	GAAGAC	2 cut(s)	469, 875
BsuRI	GGCC	2 cut(s)	201, 666
BtgI	CCRYGG	3 cut(s)	303, 674, 733
BtgZI	GCGATG	1 cut(s)	389
BtsCI	GGATG	7 cut(s)	177, 346, 496, 594, 713, 946, 994
BtsIMutI	CAGTG	3 cut(s)	128, 348, 1068
BveI	ACCTGC	1 cut(s)	232
Cac8I	GCNNGC	2 cut(s)	181, 963
CaiI	CAGNNNCTG	1 cut(s)	1051
CfoI	GCGC	1 cut(s)	185
Cfr10I	RCCGGY	2 cut(s)	179, 744
Cfr13I	GGNCC	1 cut(s)	199
Csp6I	GTAC	3 cut(s)	346, 390, 487
CviAII	CATG	6 cut(s)	154, 304, 419, 436, 734, 1013
CviQI	GTAC	3 cut(s)	346, 390, 487
DdeI	CTNAG	2 cut(s)	23, 845
DinI	GGCGCC	1 cut(s)	184
DpnI	GATC	2 cut(s)	591, 731
DpnII	GATC	2 cut(s)	589, 729
EaeI	YGGCCR	1 cut(s)	664
Eam1104I	CTCTTC	1 cut(s)	134
EarI	CTCTTC	1 cut(s)	134
Ecl136II	GAGCTC	1 cut(s)	112
Eco130I	CCWWGG	2 cut(s)	303, 733
Eco24I	GRGCYC	1 cut(s)	114
Eco53kI	GAGCTC	1 cut(s)	112
Eco57I	CTGAAG	2 cut(s)	149, 470
Eco88I	CYCGRG	1 cut(s)	953
EcoICRI	GAGCTC	1 cut(s)	112
EcoRII	CCWGG	2 cut(s)	499, 1050
EcoT14I	CCWWGG	2 cut(s)	303, 733
EcoT38I	GRGCYC	1 cut(s)	114
EgeI	GGCGCC	1 cut(s)	184
EheI	GGCGCC	1 cut(s)	184
ErhI	CCWWGG	2 cut(s)	303, 733
Esp3I	CGTCTC	1 cut(s)	641
FaeI	CATG	6 cut(s)	157, 307, 422, 439, 737, 1016
FaqI	GGGAC	1 cut(s)	284
FatI	CATG	6 cut(s)	153, 303, 418, 435, 733, 1012
FauI	CCCGC	1 cut(s)	286
Fnu4HI	GCNGC	2 cut(s)	380, 803
FokI	GGATG	7 cut(s)	184, 353, 483, 581, 700, 953, 1001
FriOI	GRGCYC	1 cut(s)	114
Fsp4HI	GCNGC	2 cut(s)	380, 803
FspBI	CTAG	4 cut(s)	32, 92, 227, 1081
GlaI	GCGC	1 cut(s)	184
GluI	GCNGC	2 cut(s)	380, 803
GsuI	CTGGAG	1 cut(s)	195
HaeII	RGCGCY	1 cut(s)	186
HaeIII	GGCC	2 cut(s)	201, 666
HapII	CCGG	4 cut(s)	180, 186, 467, 745
HhaI	GCGC	1 cut(s)	185
Hin1I	GRCGYC	1 cut(s)	183
Hin1II	CATG	6 cut(s)	157, 307, 422, 439, 737, 1016
Hin6I	GCGC	1 cut(s)	183
HinP1I	GCGC	1 cut(s)	183
HincII	GTYRAC	1 cut(s)	415
HindII	GTYRAC	1 cut(s)	415
HindIII	AAGCTT	2 cut(s)	555, 959
HinfI	GANTC	4 cut(s)	215, 230, 297, 562
HpaII	CCGG	4 cut(s)	180, 186, 467, 745
HphI	GGTGA	1 cut(s)	341
Hpy166II	GTNNAC	4 cut(s)	247, 348, 415, 529
Hpy188I	TCNGA	4 cut(s)	55, 168, 238, 1047
Hpy188III	TCNNGA	6 cut(s)	212, 271, 427, 598, 932, 1004
Hpy8I	GTNNAC	4 cut(s)	247, 348, 415, 529
HpyAV	CCTTC	4 cut(s)	282, 601, 640, 975
HpyCH4III	ACNGT	3 cut(s)	352, 886, 1072
HpyCH4IV	ACGT	1 cut(s)	568
HpyCH4V	TGCA	1 cut(s)	965
HpyF10VI	GCNNNNNNNGC	2 cut(s)	191, 445
HpyF3I	CTNAG	2 cut(s)	23, 845
HpySE526I	ACGT	1 cut(s)	568
Hsp92I	GRCGYC	1 cut(s)	183
Hsp92II	CATG	6 cut(s)	157, 307, 422, 439, 737, 1016
HspAI	GCGC	1 cut(s)	183
KasI	GGCGCC	1 cut(s)	182
KpnI	GGTACC	1 cut(s)	490
KroI	GCCGGC	1 cut(s)	179
KroNI	GCCGGC	1 cut(s)	181
Kzo9I	GATC	2 cut(s)	589, 729
LmnI	GCTCC	2 cut(s)	117, 191
Lsp1109I	GCAGC	1 cut(s)	789
LweI	GCATC	9 cut(s)	264, 312, 366, 505, 616, 648, 709, 979, 1009
MaeI	CTAG	4 cut(s)	32, 92, 227, 1081
MaeII	ACGT	1 cut(s)	568
MaeIII	GTNAC	1 cut(s)	945
MalI	GATC	2 cut(s)	591, 731
MboI	GATC	2 cut(s)	589, 729
MboII	GAAGA	7 cut(s)	151, 474, 832, 880, 910, 1006, 1019
MfeI	CAATTG	1 cut(s)	966
MhlI	GDGCHC	1 cut(s)	114
MluCI	AATT	2 cut(s)	769, 966
Mly113I	GGCGCC	1 cut(s)	183
MlyI	GAGTC	1 cut(s)	306
MmeI	TCCRAC	2 cut(s)	1044, 1059
MreI	CGCCGGCG	1 cut(s)	179
MroNI	GCCGGC	1 cut(s)	179
MseI	TTAA	5 cut(s)	534, 642, 705, 768, 807
MslI	CAYNNNNRTG	2 cut(s)	308, 1073
MspI	CCGG	4 cut(s)	180, 186, 467, 745
MspR9I	CCNGG	2 cut(s)	501, 1052
MunI	CAATTG	1 cut(s)	966
MvaI	CCWGG	2 cut(s)	501, 1052
MwoI	GCNNNNNNNGC	2 cut(s)	191, 445
NaeI	GCCGGC	1 cut(s)	181
NarI	GGCGCC	1 cut(s)	183
NcoI	CCATGG	2 cut(s)	303, 733
NdeII	GATC	2 cut(s)	589, 729
NgoMIV	GCCGGC	1 cut(s)	179
NlaIII	CATG	6 cut(s)	157, 307, 422, 439, 737, 1016
NlaIV	GGNNCC	2 cut(s)	184, 488
NmeAIII	GCCGAG	1 cut(s)	642
NspI	RCATGY	2 cut(s)	439, 1016
NspV	TTCGAA	1 cut(s)	866
OliI	CACNNNNGTG	1 cut(s)	1073
PasI	CCCWGGG	1 cut(s)	1051
PciI	ACATGT	1 cut(s)	1012
PcsI	WCGNNNNNNNCGW	1 cut(s)	1071
PdiI	GCCGGC	1 cut(s)	181
PfeI	GAWTC	3 cut(s)	215, 230, 562
PflFI	GACNNNGTC	1 cut(s)	1070
PfoI	TCCNGGA	1 cut(s)	499
PkrI	GCNGC	2 cut(s)	381, 804
PleI	GAGTC	1 cut(s)	305
PluTI	GGCGCC	1 cut(s)	186
PpsI	GAGTC	1 cut(s)	305
PscI	ACATGT	1 cut(s)	1012
PshBI	ATTAAT	2 cut(s)	534, 768
PsiI	TTATAA	1 cut(s)	68
Psp124BI	GAGCTC	1 cut(s)	114
Psp6I	CCWGG	2 cut(s)	499, 1050
PspGI	CCWGG	2 cut(s)	499, 1050
PspN4I	GGNNCC	2 cut(s)	184, 488
PspPI	GGNCC	1 cut(s)	199
PstNI	CAGNNNCTG	1 cut(s)	1051
PsyI	GACNNNGTC	1 cut(s)	1070
RsaI	GTAC	3 cut(s)	347, 391, 488
RsaNI	GTAC	3 cut(s)	346, 390, 487
RseI	CAYNNNNRTG	2 cut(s)	308, 1073
SacI	GAGCTC	1 cut(s)	114
SaqAI	TTAA	5 cut(s)	534, 642, 705, 768, 807
SatI	GCNGC	2 cut(s)	380, 803
Sau3AI	GATC	2 cut(s)	589, 729
Sau96I	GGNCC	1 cut(s)	199
SchI	GAGTC	1 cut(s)	306
ScrFI	CCNGG	2 cut(s)	501, 1052
SduI	GDGCHC	1 cut(s)	114
SfaNI	GCATC	9 cut(s)	264, 312, 366, 505, 616, 648, 709, 979, 1009
SfcI	CTRYAG	1 cut(s)	759
SfoI	GGCGCC	1 cut(s)	184
SfuI	TTCGAA	1 cut(s)	866
SgrAI	CRCCGGYG	1 cut(s)	179
SmiMI	CAYNNNNRTG	2 cut(s)	308, 1073
SmlI	CTYRAG	3 cut(s)	269, 278, 788
SmoI	CTYRAG	3 cut(s)	269, 278, 788
SpeI	ACTAGT	1 cut(s)	31
Sse9I	AATT	2 cut(s)	769, 966
SsiI	CCGC	2 cut(s)	293, 380
SspDI	GGCGCC	1 cut(s)	182
SspI	AATATT	1 cut(s)	59
SspMI	CTAG	4 cut(s)	32, 92, 227, 1081
SstI	GAGCTC	1 cut(s)	114
StyD4I	CCNGG	2 cut(s)	499, 1050
StyI	CCWWGG	2 cut(s)	303, 733
TaaI	ACNGT	3 cut(s)	352, 886, 1072
TaiI	ACGT	1 cut(s)	571
TaqI	TCGA	6 cut(s)	355, 363, 405, 866, 1005, 1065
TasI	AATT	2 cut(s)	769, 966
TatI	WGTACW	1 cut(s)	345
TauI	GCSGC	1 cut(s)	382
TfiI	GAWTC	3 cut(s)	215, 230, 562
Tru1I	TTAA	5 cut(s)	534, 642, 705, 768, 807
Tru9I	TTAA	5 cut(s)	534, 642, 705, 768, 807
TscAI	CASTG	3 cut(s)	135, 355, 1075
TseI	GCWGC	1 cut(s)	802
TspDTI	ATGAA	1 cut(s)	911
TspGWI	ACGGA	2 cut(s)	300, 623
TspRI	CASTG	3 cut(s)	135, 355, 1075
Tth111I	GACNNNGTC	1 cut(s)	1070
VspI	ATTAAT	2 cut(s)	534, 768
XceI	RCATGY	2 cut(s)	439, 1016
XcmI	CCANNNNNNNNNTGG	2 cut(s)	310, 497
XspI	CTAG	4 cut(s)	32, 92, 227, 1081

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.