pycom15g09000

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010492
Physical Location & Seq
Reverse (-)
5781231 .. 5783989
2759 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1146 bp
ATGAATCTGCTCGAGGATAGACTTCAGCATGCAAATGGTGCTGTTGTCTTAGCAACCACTAAAGTATTTCTTCAGTTGACTCTGTCAATGACTGATGTACATCAGCAGGTATATGAACGCATTAAAGCTCCTCTCCTGACCTTAGTGAGTTCAGGAAGTCCAGAGCAATCTTATGCAGTTCTAAGTCATCTGCATCTTTTGGTGATGCGTGCACCTTTCATATTTTCTTCAGACTATAAACACTTTTATTGCCAGTACAATGAGCCATCTTATGTTAAAAGGTTGAAGCTTGAGATGTTGACTGCAGTGGCAAATGAGAGTAACACTTATGAAATTGTGACGGAATTATGTGAATATGCTGCAAATGTTGATATTCCCATTGCAAGAGAGTCAATCCGTGCAGTTGGGAAAATAGCTCTACAACAGTATGATGTGAATGCAATTGTGGATCGACTTCTTCAATTTCTGGAGATGGAAAAGGACTATGTAACTGCTGAGGCTCTGGTTCTTGTAAAAGATCTGCTGAGGAAATATCCACAATGGAGTCAGGATTGTATTGCGGTTGTTGGGAACATTAGCAGCAAAAATGTTCAAGAACCCAAGGCCAAGGCAGCTCTTATATGGATGCTGGGGGAGTATTCTCAGGAAATGCATGATGCACCTTATATTTTAGAGGGTTTGATTGAAAATTGGGAAGATGAGCATTCCGCTGAGGTTCGCTTACATCTACTAACAGCAGTAATGAAGTGTTTTTTTAAGAGGCCACCTGAGACTCAGAAATCCCTGGGAGCTGCACTGGCTGCAGGTCTTGCTGATTTTCATCAGGATGTTCATGATAGAGCCTTATTCTACTACAGGCTTTTGCAATATAAAATAAGTGTAGCAGAACAAGTGGTGAACCCTCCAAAGCAAGCTGTTTCTGTCTTTGCTGATACCCAGAGCAGTGAGGAATCATCGATCGGATTTTCGATGAATTTAACAGTTTATCCGTGGTATATCAGAAGCCTTCGTACATGTTTACTTATAAGGAACACAGAGGCCCATTTGAGTTTTCAGATGAAATTGGAAATGTATCTATTGGGACAGAATCTGCGGATACTGTTGTTCCAAACAGAGTCGAAGCAAATGATAAGGATCTGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019899 GO:0019900 GO:0019901 GO:0043424
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N B2-adapt-app_C

Protein Analysis

382

Amino Acids

43.64

Weight (kDa)

5.91

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 1 - 289 6.4e-43 Adaptin N terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11490 AT5G11490 AT5G11490
fragaria_vesca FvH4_2g36170 FvH4_2g36170 FvH4_2g36170
malus_domestica MD08G1118900.v1.1 MD15G1098200.v1.1
prunus_persica Prupe.1G453100_v2.0.a1
pyrus_communis pycom08g09930 pycom15g09000
rosa_chinensis RchiOBHm_Chr6g0305701
rosa_laevigata RLG00000010867
rosa_multiflora Rmu_sc0001809.1_g000035
rosa_roxburghii Rroxscaffold_7G00162430
rosa_rugosa Rorug06G0343300
rosa_samantha Rh6AG454800 Rh6BG446700 Rh6CG468700 Rh6DG455300
rosa_wichuraiana Rw6G039660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1025
Acc36I ACCTGC 2 cut(s) 97, 794
AciI CCGC 3 cut(s) 560, 708, 1093
AclWI GGATC 2 cut(s) 456, 1142
AcsI RAATTY 1 cut(s) 973
AcuI CTGAAG 3 cut(s) 8, 56, 213
AfaI GTAC 3 cut(s) 99, 257, 1012
AflIII ACRYGT 1 cut(s) 1013
AgsI TTSAA 4 cut(s) 286, 461, 593, 686
AjnI CCWGG 1 cut(s) 783
AluBI AGCT 6 cut(s) 128, 289, 416, 614, 791, 914
AluI AGCT 6 cut(s) 128, 289, 416, 614, 791, 914
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 764
Alw44I GTGCAC 1 cut(s) 210
AlwI GGATC 2 cut(s) 456, 1142
AlwNI CAGNNNCTG 1 cut(s) 1090
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 3 cut(s) 603, 761, 1038
ApaLI GTGCAC 1 cut(s) 210
ApeKI GCWGC 5 cut(s) 359, 579, 611, 791, 800
ApoI RAATTY 1 cut(s) 973
AspS9I GGNCC 1 cut(s) 1039
AsuHPI GGTGA 2 cut(s) 214, 907
AvaI CYCGRG 1 cut(s) 11
BaeGI GKGCMC 1 cut(s) 214
BaeI ACNNNNGTAYC 2 cut(s) 1088, 1121
BarI GAAGNNNNNNTAC 2 cut(s) 994, 1026
Bbv12I GWGCWC 1 cut(s) 214
BbvCI CCTCAGC 3 cut(s) 495, 524, 711
BbvI GCAGC 5 cut(s) 346, 591, 623, 778, 787
BccI CCATC 2 cut(s) 274, 466
BciT130I CCWGG 1 cut(s) 785
BciVI GTATCC 1 cut(s) 1089
BcoDI GTCTC 1 cut(s) 764
BfmI CTRYAG 3 cut(s) 303, 801, 853
BfuAI ACCTGC 2 cut(s) 97, 794
BfuI GTATCC 1 cut(s) 1089
BglII AGATCT 1 cut(s) 517
BisI GCNGC 5 cut(s) 360, 580, 612, 792, 801
BlsI GCNGC 5 cut(s) 361, 581, 613, 793, 802
Bme1390I CCNGG 1 cut(s) 785
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 1039
BmrFI CCNGG 1 cut(s) 785
BmsI GCATC 4 cut(s) 195, 202, 615, 646
BpmI CTGGAG 1 cut(s) 488
Bpu10I CCTNAGC 3 cut(s) 495, 524, 711
BpuEI CTTGAG 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 956
BsaBI GATNNNNATC 3 cut(s) 99, 819, 1133
BsaJI CCNNGG 5 cut(s) 600, 606, 783, 784, 989
Bse1I ACTGG 2 cut(s) 253, 801
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 3 cut(s) 99, 819, 1133
BseBI CCWGG 1 cut(s) 785
BseCI ATCGAT 1 cut(s) 956
BseDI CCNNGG 5 cut(s) 600, 606, 783, 784, 989
BseGI GGATG 2 cut(s) 630, 832
BseJI GATNNNNATC 3 cut(s) 99, 819, 1133
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 6 cut(s) 486, 515, 656, 702, 759, 788
BseNI ACTGG 2 cut(s) 253, 801
BseRI GAGGAG 1 cut(s) 120
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 5 cut(s) 346, 591, 623, 778, 787
BseYI CCCAGC 1 cut(s) 628
BsgI GTGCAG 2 cut(s) 420, 777
Bsh1285I CGRYCG 1 cut(s) 960
BshFI GGCC 3 cut(s) 605, 763, 1040
BshVI ATCGAT 1 cut(s) 956
BsiEI CGRYCG 1 cut(s) 960
BsiHKAI GWGCWC 1 cut(s) 214
BsiHKCI CYCGRG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 1095
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 1095
BsmI GAATGC 2 cut(s) 442, 703
BsnI GGCC 3 cut(s) 605, 763, 1040
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 214
Bsp1407I TGTACA 1 cut(s) 97
Bsp143I GATC 4 cut(s) 448, 517, 957, 1134
BspACI CCGC 3 cut(s) 560, 708, 1093
BspANI GGCC 3 cut(s) 605, 763, 1040
BspCNI CTCAG 6 cut(s) 487, 516, 655, 703, 760, 787
BspDI ATCGAT 1 cut(s) 956
BspHI TCATGA 1 cut(s) 832
BspMAI CTGCAG 2 cut(s) 307, 805
BspMI ACCTGC 2 cut(s) 97, 794
BspPI GGATC 2 cut(s) 456, 1142
BsrDI GCAATG 1 cut(s) 378
BsrGI TGTACA 1 cut(s) 97
BsrI ACTGG 2 cut(s) 253, 801
BssECI CCNNGG 5 cut(s) 600, 606, 783, 784, 989
BssMI GATC 4 cut(s) 448, 517, 957, 1134
BssT1I CCWWGG 2 cut(s) 600, 606
Bst2UI CCWGG 1 cut(s) 785
Bst4CI ACNGT 3 cut(s) 426, 982, 1101
BstAPI GCANNNNNTGC 3 cut(s) 38, 800, 809
BstAUI TGTACA 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 30, 210, 912
BstDEI CTNAG 9 cut(s) 49, 142, 182, 495, 524, 642, 711, 768, 774
BstDSI CCRYGG 1 cut(s) 989
BstF5I GGATG 2 cut(s) 630, 832
BstKTI GATC 4 cut(s) 451, 520, 960, 1137
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 4 cut(s) 448, 517, 957, 1134
BstMCI CGRYCG 1 cut(s) 960
BstMWI GCNNNNNNNGC 5 cut(s) 38, 611, 797, 800, 809
BstNI CCWGG 1 cut(s) 785
BstNSI RCATGY 2 cut(s) 32, 1017
BstSCI CCNGG 1 cut(s) 783
BstSFI CTRYAG 3 cut(s) 303, 801, 853
BstSLI GKGCMC 1 cut(s) 214
BstV1I GCAGC 5 cut(s) 346, 591, 623, 778, 787
BstX2I RGATCY 2 cut(s) 517, 1134
BstYI RGATCY 2 cut(s) 517, 1134
Bsu15I ATCGAT 1 cut(s) 956
BsuI GTATCC 1 cut(s) 1089
BsuRI GGCC 3 cut(s) 605, 763, 1040
BsuTUI ATCGAT 1 cut(s) 956
BtgI CCRYGG 1 cut(s) 989
BtsCI GGATG 2 cut(s) 630, 832
BtsI GCAGTG 2 cut(s) 312, 949
BtsIMutI CAGTG 3 cut(s) 312, 794, 949
BveI ACCTGC 2 cut(s) 97, 794
Cac8I GCNNGC 3 cut(s) 30, 210, 912
CaiI CAGNNNCTG 1 cut(s) 1090
CciI TCATGA 1 cut(s) 832
Cfr13I GGNCC 1 cut(s) 1039
ClaI ATCGAT 1 cut(s) 956
Csp6I GTAC 3 cut(s) 98, 256, 1011
CviAII CATG 4 cut(s) 29, 653, 833, 1014
CviQI GTAC 3 cut(s) 98, 256, 1011
DdeI CTNAG 9 cut(s) 49, 142, 182, 495, 524, 642, 711, 768, 774
DpnI GATC 4 cut(s) 450, 519, 959, 1136
DpnII GATC 4 cut(s) 448, 517, 957, 1134
Eco130I CCWWGG 2 cut(s) 600, 606
Eco57I CTGAAG 3 cut(s) 8, 56, 213
Eco88I CYCGRG 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 783
EcoT14I CCWWGG 2 cut(s) 600, 606
EcoT22I ATGCAT 1 cut(s) 654
ErhI CCWWGG 2 cut(s) 600, 606
FaeI CATG 4 cut(s) 32, 656, 836, 1017
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FaqI GGGAC 1 cut(s) 1095
FatI CATG 4 cut(s) 28, 652, 832, 1013
Fnu4HI GCNGC 5 cut(s) 360, 580, 612, 792, 801
FokI GGATG 2 cut(s) 637, 839
Fsp4HI GCNGC 5 cut(s) 360, 580, 612, 792, 801
GluI GCNGC 5 cut(s) 360, 580, 612, 792, 801
GsaI CCCAGC 1 cut(s) 632
GsuI CTGGAG 1 cut(s) 488
HaeIII GGCC 3 cut(s) 605, 763, 1040
Hin1II CATG 4 cut(s) 32, 656, 836, 1017
HincII GTYRAC 2 cut(s) 78, 300
HindII GTYRAC 2 cut(s) 78, 300
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 8 cut(s) 4, 79, 389, 544, 772, 950, 1087, 1115
HphI GGTGA 2 cut(s) 214, 907
Hpy166II GTNNAC 5 cut(s) 78, 212, 300, 898, 1019
Hpy188I TCNGA 5 cut(s) 232, 777, 962, 1001, 1056
Hpy188III TCNNGA 9 cut(s) 136, 153, 161, 467, 548, 593, 644, 824, 833
Hpy8I GTNNAC 5 cut(s) 78, 212, 300, 898, 1019
HpyAV CCTTC 1 cut(s) 1016
HpyCH4III ACNGT 3 cut(s) 426, 982, 1101
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 611, 797, 800, 809
HpyF3I CTNAG 9 cut(s) 49, 142, 182, 495, 524, 642, 711, 768, 774
Hsp92II CATG 4 cut(s) 32, 656, 836, 1017
Kzo9I GATC 4 cut(s) 448, 517, 957, 1134
LmnI GCTCC 2 cut(s) 133, 788
Lsp1109I GCAGC 5 cut(s) 346, 591, 623, 778, 787
LweI GCATC 4 cut(s) 195, 202, 615, 646
MaeIII GTNAC 3 cut(s) 320, 337, 487
MalI GATC 4 cut(s) 450, 519, 959, 1136
MboI GATC 4 cut(s) 448, 517, 957, 1134
MboII GAAGA 4 cut(s) 62, 219, 449, 707
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 2 cut(s) 517, 1134
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 7 cut(s) 333, 344, 441, 461, 688, 973, 1061
MlyI GAGTC 5 cut(s) 73, 398, 553, 766, 1124
Mph1103I ATGCAT 1 cut(s) 654
MseI TTAA 5 cut(s) 123, 276, 756, 977, 1144
MslI CAYNNNNRTG 2 cut(s) 33, 825
MspA1I CMGCKG 1 cut(s) 710
MspR9I CCNGG 1 cut(s) 785
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 2 cut(s) 442, 703
MvaI CCWGG 1 cut(s) 785
MwoI GCNNNNNNNGC 5 cut(s) 38, 611, 797, 800, 809
NdeII GATC 4 cut(s) 448, 517, 957, 1134
NlaIII CATG 4 cut(s) 32, 656, 836, 1017
NmuCI GTSAC 1 cut(s) 337
NsiI ATGCAT 1 cut(s) 654
NspI RCATGY 2 cut(s) 32, 1017
PaeI GCATGC 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 11
PagI TCATGA 1 cut(s) 832
PasI CCCWGGG 1 cut(s) 784
PciI ACATGT 1 cut(s) 1013
PctI GAATGC 2 cut(s) 442, 703
PfeI GAWTC 3 cut(s) 4, 950, 1087
PflFI GACNNNGTC 1 cut(s) 82
PkrI GCNGC 5 cut(s) 361, 581, 613, 793, 802
Ple19I CGATCG 1 cut(s) 960
PleI GAGTC 5 cut(s) 73, 397, 552, 766, 1123
PpsI GAGTC 5 cut(s) 73, 397, 552, 766, 1123
PscI ACATGT 1 cut(s) 1013
PsiI TTATAA 1 cut(s) 1025
Psp6I CCWGG 1 cut(s) 783
PspFI CCCAGC 1 cut(s) 628
PspGI CCWGG 1 cut(s) 783
PspPI GGNCC 1 cut(s) 1039
PspXI VCTCGAGB 1 cut(s) 11
PstI CTGCAG 2 cut(s) 307, 805
PstNI CAGNNNCTG 1 cut(s) 1090
PsuI RGATCY 2 cut(s) 517, 1134
PsyI GACNNNGTC 1 cut(s) 82
PvuI CGATCG 1 cut(s) 960
RsaI GTAC 3 cut(s) 99, 257, 1012
RsaNI GTAC 3 cut(s) 98, 256, 1011
RseI CAYNNNNRTG 2 cut(s) 33, 825
SaqAI TTAA 5 cut(s) 123, 276, 756, 977, 1144
SatI GCNGC 5 cut(s) 360, 580, 612, 792, 801
Sau3AI GATC 4 cut(s) 448, 517, 957, 1134
Sau96I GGNCC 1 cut(s) 1039
SchI GAGTC 5 cut(s) 73, 398, 553, 766, 1124
ScrFI CCNGG 1 cut(s) 785
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 4 cut(s) 195, 202, 615, 646
SfcI CTRYAG 3 cut(s) 303, 801, 853
Sfr274I CTCGAG 1 cut(s) 11
SlaI CTCGAG 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 33, 825
SmlI CTYRAG 2 cut(s) 11, 290
SmoI CTYRAG 2 cut(s) 11, 290
SphI GCATGC 1 cut(s) 32
Sse9I AATT 7 cut(s) 333, 344, 441, 461, 688, 973, 1061
SsiI CCGC 3 cut(s) 560, 708, 1093
StyD4I CCNGG 1 cut(s) 783
StyI CCWWGG 2 cut(s) 600, 606
TaaI ACNGT 3 cut(s) 426, 982, 1101
TaqI TCGA 5 cut(s) 12, 451, 956, 968, 1118
TasI AATT 7 cut(s) 333, 344, 441, 461, 688, 973, 1061
TatI WGTACW 2 cut(s) 97, 255
TfiI GAWTC 3 cut(s) 4, 950, 1087
Tru1I TTAA 5 cut(s) 123, 276, 756, 977, 1144
Tru9I TTAA 5 cut(s) 123, 276, 756, 977, 1144
TscAI CASTG 3 cut(s) 312, 801, 949
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 5 cut(s) 359, 579, 611, 791, 800
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 9 cut(s) 17, 129, 208, 345, 758, 809, 821, 986, 1073
TspGWI ACGGA 3 cut(s) 356, 386, 978
TspRI CASTG 3 cut(s) 312, 801, 949
Tth111I GACNNNGTC 1 cut(s) 82
VneI GTGCAC 1 cut(s) 210
XapI RAATTY 1 cut(s) 973
XceI RCATGY 2 cut(s) 32, 1017
XhoI CTCGAG 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.