pycom15g09880

Cenp-O kinetochore centromere component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008371
Physical Location & Seq
Forward (+)
6424841 .. 6430516
5676 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1065 bp
ATGGATTACAGCGACGACGCAGAAGAGCATTTCAGGCGGAGACCGAGGAAGATGGCGTATCTCGGAGACCCATCTCGCAGGCAGCTTGAATCGCTGAGCGGACGTTCCTATGCCCTAGGAGAAATGGGTTATATGCAAGAGGAAGATATTTCTTTAGACGCCATACGAGCTAGATGGACAAATGTTCTCAAAAGGCATAGCGAGTTAGCAGAGCGTCTTTCTAGGGATGCTGACAAGATAATTTATGAGCGTCTACAGAAGGAATTTGAAGCTGCACGGGCTTCACAAACTCAAGAGACGTATTTGGATGGTGAACAATGGAACGATGGACTACTAGCAACAATAAGAGAGCGGGTTCACATGGAGGCTGAAAGAAAGGCAATGGAGGGAGACATAAACATGTTGCCTGGTCAAGAAAAAATTACGTATAAAACCGGAAATAAGGTGATTTGCTGTTTGGAAGGGGCAAGAATTGGCATACTGTATGAGACATCTTATGCAGGAGAACCTTGTGACCTATACCATTGTGTGCTTGAGAGCAAGTCATTTCTTGAGAAGATGACAGTCCTTGAACACACCATTCCGTACTTCTTGCCAATACGAGAAGCAGAAAATGATCTTCTTTCCTCCAATGCTATGAAATTTATAGATTACATTGGAGAGATGTTACAGGCTTATGTGGACAGAAGGGAACAGGTTAGGCTTGTCAAGGAGTTATATGGGAATCAGATCAAAGAAATTCATTTTACCCTTCCCTACAGCTTAATCGTCTTTTCAATACTTGATTTTGATTGCAAGGTGGTTGTGAAGCTTAAATATGCAGAGCTTGTTTCTGTGCTTCCAACTCATGTCAGTGTGGTAGCGTGGCCAGTGTGTCAACCCAGAAAAAGCAGTCTAAATATATCATCCATGAACAAAAAGGAAAACGGAACATTGGAAGGTCCAACCACCCTCTTCTATGCAGAAGATGCCTTGCGAACTAAGAGCTTACCAGAAGCATACGCTGACATAGTGCTGAATTTGCCCCAAACTCTTCGGGAAATATGCGAGGGGAACTCTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CENP-O

Protein Analysis

355

Amino Acids

40.71

Weight (kDa)

5.33

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-O PF09496 155 - 342 2.6e-20 Cenp-O kinetochore centromere component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 99, 352
AccI GTMKAC 1 cut(s) 253
AciI CCGC 3 cut(s) 37, 99, 352
AcoI YGGCCR 1 cut(s) 866
AcsI RAATTY 4 cut(s) 263, 641, 738, 1018
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 587
AflIII ACRYGT 1 cut(s) 399
AgsI TTSAA 4 cut(s) 89, 269, 572, 777
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 7 cut(s) 85, 170, 272, 762, 811, 826, 987
AluI AGCT 7 cut(s) 85, 170, 272, 762, 811, 826, 987
Alw26I GTCTC 5 cut(s) 34, 60, 290, 384, 482
AoxI GGCC 1 cut(s) 866
ApeKI GCWGC 2 cut(s) 82, 272
ApoI RAATTY 4 cut(s) 263, 641, 738, 1018
AspA2I CCTAGG 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 941
AsuHPI GGTGA 2 cut(s) 323, 457
AvaII GGWCC 1 cut(s) 941
AvrII CCTAGG 1 cut(s) 115
BalI TGGCCA 1 cut(s) 868
BbvI GCAGC 2 cut(s) 94, 259
BccI CCATC 5 cut(s) 46, 79, 168, 302, 320
BciT130I CCWGG 1 cut(s) 408
BcoDI GTCTC 5 cut(s) 34, 60, 290, 384, 482
BfaI CTAG 4 cut(s) 116, 171, 222, 335
BfmI CTRYAG 2 cut(s) 254, 757
BisI GCNGC 2 cut(s) 83, 273
BlnI CCTAGG 1 cut(s) 115
BlpI GCTNAGC 1 cut(s) 95
BlsI GCNGC 2 cut(s) 84, 274
Bme1390I CCNGG 1 cut(s) 408
Bme18I GGWCC 1 cut(s) 941
BmgT120I GGNCC 1 cut(s) 941
BmrFI CCNGG 1 cut(s) 408
BmsI GCATC 2 cut(s) 217, 958
BplI GAGNNNNNCTC 1 cut(s) 1040
Bpu1102I GCTNAGC 1 cut(s) 95
BpuEI CTTGAG 3 cut(s) 276, 554, 572
BsaAI YACGTR 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 2 cut(s) 34, 60
BsaJI CCNNGG 2 cut(s) 44, 115
BsaWI WCCGGW 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 869
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 1 cut(s) 408
BseDI CCNNGG 2 cut(s) 44, 115
BseGI GGATG 3 cut(s) 232, 313, 905
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 1 cut(s) 869
BseXI GCAGC 2 cut(s) 94, 259
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 1 cut(s) 868
BsiSI CCGG 1 cut(s) 435
BsmAI GTCTC 5 cut(s) 34, 60, 290, 384, 482
BsmBI CGTCTC 1 cut(s) 290
BsnI GGCC 1 cut(s) 868
Bso31I GGTCTC 2 cut(s) 34, 60
Bsp143I GATC 2 cut(s) 616, 729
Bsp1720I GCTNAGC 1 cut(s) 95
BspACI CCGC 3 cut(s) 37, 99, 352
BspANI GGCC 1 cut(s) 868
BspCNI CTCAG 1 cut(s) 87
BspQI GCTCTTC 1 cut(s) 18
BspTNI GGTCTC 2 cut(s) 34, 60
BsrBI CCGCTC 2 cut(s) 99, 352
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 1 cut(s) 869
BssECI CCNNGG 2 cut(s) 44, 115
BssMI GATC 2 cut(s) 616, 729
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 2 cut(s) 483, 565
Bst6I CTCTTC 3 cut(s) 18, 959, 1038
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 968
BstBAI YACGTR 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 2 cut(s) 95, 981
BstF5I GGATG 3 cut(s) 232, 313, 905
BstKTI GATC 2 cut(s) 619, 732
BstMAI GTCTC 5 cut(s) 34, 60, 290, 384, 482
BstMBI GATC 2 cut(s) 616, 729
BstMWI GCNNNNNNNGC 6 cut(s) 34, 91, 167, 278, 968, 1021
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 406
BstSFI CTRYAG 2 cut(s) 254, 757
BstSNI TACGTA 1 cut(s) 426
BstV1I GCAGC 2 cut(s) 94, 259
BsuRI GGCC 1 cut(s) 868
BtsCI GGATG 3 cut(s) 232, 313, 905
BtsIMutI CAGTG 2 cut(s) 859, 876
Cac8I GCNNGC 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 941
CseI GACGC 4 cut(s) 26, 167, 203, 239
Csp6I GTAC 1 cut(s) 586
CviAII CATG 4 cut(s) 361, 400, 848, 910
CviQI GTAC 1 cut(s) 586
DdeI CTNAG 2 cut(s) 95, 981
DpnI GATC 2 cut(s) 618, 731
DpnII GATC 2 cut(s) 616, 729
EaeI YGGCCR 1 cut(s) 866
Eam1104I CTCTTC 3 cut(s) 18, 959, 1038
EarI CTCTTC 3 cut(s) 18, 959, 1038
EciI GGCGGA 1 cut(s) 52
Eco105I TACGTA 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 115
Eco31I GGTCTC 2 cut(s) 34, 60
Eco47I GGWCC 1 cut(s) 941
EcoRII CCWGG 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 115
Esp3I CGTCTC 1 cut(s) 290
FaeI CATG 4 cut(s) 364, 403, 851, 913
FatI CATG 4 cut(s) 360, 399, 847, 909
FauI CCCGC 1 cut(s) 345
FblI GTMKAC 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 83, 273
FokI GGATG 3 cut(s) 239, 320, 892
Fsp4HI GCNGC 2 cut(s) 83, 273
FspBI CTAG 4 cut(s) 116, 171, 222, 335
GluI GCNGC 2 cut(s) 83, 273
HaeIII GGCC 1 cut(s) 868
HapII CCGG 1 cut(s) 435
HgaI GACGC 4 cut(s) 26, 167, 203, 239
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 4 cut(s) 364, 403, 851, 913
HincII GTYRAC 1 cut(s) 878
HindII GTYRAC 1 cut(s) 878
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 2 cut(s) 89, 724
HpaII CCGG 1 cut(s) 435
HphI GGTGA 2 cut(s) 323, 457
Hpy166II GTNNAC 5 cut(s) 254, 314, 358, 682, 878
Hpy188I TCNGA 2 cut(s) 65, 729
Hpy188III TCNNGA 4 cut(s) 293, 413, 551, 1037
Hpy8I GTNNAC 5 cut(s) 254, 314, 358, 682, 878
Hpy99I CGWCG 2 cut(s) 17, 20
HpyAV CCTTC 5 cut(s) 253, 455, 681, 761, 932
HpyCH4III ACNGT 2 cut(s) 483, 565
HpyCH4IV ACGT 3 cut(s) 103, 299, 425
HpyCH4V TGCA 6 cut(s) 136, 275, 500, 795, 821, 962
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 91, 167, 278, 968, 1021
HpyF3I CTNAG 2 cut(s) 95, 981
HpySE526I ACGT 3 cut(s) 103, 299, 425
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 4 cut(s) 364, 403, 851, 913
Kzo9I GATC 2 cut(s) 616, 729
LguI GCTCTTC 1 cut(s) 18
Lsp1109I GCAGC 2 cut(s) 94, 259
LweI GCATC 2 cut(s) 217, 958
MaeI CTAG 4 cut(s) 116, 171, 222, 335
MaeII ACGT 3 cut(s) 103, 299, 425
MaeIII GTNAC 2 cut(s) 512, 666
MalI GATC 2 cut(s) 618, 731
MbiI CCGCTC 2 cut(s) 99, 352
MboI GATC 2 cut(s) 616, 729
MboII GAAGA 9 cut(s) 35, 61, 155, 568, 611, 946, 977, 1025, 1050
MlsI TGGCCA 1 cut(s) 868
MluCI AATT 7 cut(s) 240, 263, 420, 471, 641, 738, 1018
MluNI TGGCCA 1 cut(s) 868
MmeI TCCRAC 2 cut(s) 866, 968
MnlI CCTC 7 cut(s) 39, 133, 358, 379, 637, 962, 1042
Mox20I TGGCCA 1 cut(s) 868
MscI TGGCCA 1 cut(s) 868
MseI TTAA 2 cut(s) 764, 813
MslI CAYNNNNRTG 2 cut(s) 398, 852
Msp20I TGGCCA 1 cut(s) 868
MspI CCGG 1 cut(s) 435
MspR9I CCNGG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 408
MwoI GCNNNNNNNGC 6 cut(s) 34, 91, 167, 278, 968, 1021
NdeII GATC 2 cut(s) 616, 729
NlaIII CATG 4 cut(s) 364, 403, 851, 913
NmuCI GTSAC 1 cut(s) 512
NspI RCATGY 1 cut(s) 403
PciI ACATGT 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 18
PfeI GAWTC 2 cut(s) 89, 724
PkrI GCNGC 2 cut(s) 84, 274
Ppu21I YACGTR 1 cut(s) 426
PscI ACATGT 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspPI GGNCC 1 cut(s) 941
RsaI GTAC 1 cut(s) 587
RsaNI GTAC 1 cut(s) 586
RseI CAYNNNNRTG 2 cut(s) 398, 852
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 764, 813
SatI GCNGC 2 cut(s) 83, 273
Sau3AI GATC 2 cut(s) 616, 729
Sau96I GGNCC 1 cut(s) 941
ScrFI CCNGG 1 cut(s) 408
SfaNI GCATC 2 cut(s) 217, 958
SfcI CTRYAG 2 cut(s) 254, 757
SinI GGWCC 1 cut(s) 941
SmiMI CAYNNNNRTG 2 cut(s) 398, 852
SmlI CTYRAG 3 cut(s) 291, 533, 551
SmoI CTYRAG 3 cut(s) 291, 533, 551
SnaBI TACGTA 1 cut(s) 426
Sse9I AATT 7 cut(s) 240, 263, 420, 471, 641, 738, 1018
SsiI CCGC 3 cut(s) 37, 99, 352
SspMI CTAG 4 cut(s) 116, 171, 222, 335
StyD4I CCNGG 1 cut(s) 406
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 2 cut(s) 483, 565
TaiI ACGT 3 cut(s) 106, 302, 428
TaqII GACCGA 1 cut(s) 58
TasI AATT 7 cut(s) 240, 263, 420, 471, 641, 738, 1018
TfiI GAWTC 2 cut(s) 89, 724
Tru1I TTAA 2 cut(s) 764, 813
Tru9I TTAA 2 cut(s) 764, 813
TscAI CASTG 2 cut(s) 859, 876
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 2 cut(s) 82, 272
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 3 cut(s) 653, 731, 926
TspGWI ACGGA 2 cut(s) 573, 942
TspRI CASTG 2 cut(s) 859, 876
VpaK11BI GGWCC 1 cut(s) 941
XapI RAATTY 4 cut(s) 263, 641, 738, 1018
XceI RCATGY 1 cut(s) 403
XmaJI CCTAGG 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 253
XspI CTAG 4 cut(s) 116, 171, 222, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.