pycom15g10000

plastid-lipid-associated protein 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009105
Physical Location & Seq
Forward (+)
6550867 .. 6552900
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 912 bp
ATGAATTCTTTTAAGAAGGCGAGATTATTTTTCACCAAACCCAAACCGGCCCCATCTCGATTCGCATTGCCTACGTGGCACCACCTCCCCCATCATCAGATAAGAGATAGCTGTGTAATCACTTCCTCCATTTTCCCCATTCGAAGCTCAGAGCTTACCTTCCATGGCCGCTGCCGTAGCCTCATCACTAGCCCTGTCAGCCTTCACCTTCAGAGGAGGCGCCGATCCCTCGAAACCAAAATTCCTCAACCTTTGCTCAAAGCCCACAAATTTGGCTTTGACTGTTTCACCCCAATCTCACAGTTGTCGGCGCAACACTTTGAGAATATTGGCCTCTGTTTCCGTCTCCGATCCTGGAGTTCGTACCGGTCCCGAAGAGCTCTCTGCGTCTATTCTTTCCAAGGTACGGTGACGAATTCGGATAGAGGAGTTCTGCTGAAAGAGGAACAACAAAAAGAGGTAGCTAAGGAGGCTGAAGAATTGCAGAACTACTGTGTTAGCGAACCAGTGAAATGCCCTCTCATTTTCGGAGACTGGGATGTGGTGTACTGCTCAGTGCCAACATCACCCGGGGGTGGCTACAGAAGTACGTTGGGGCGCCTTTTTTTTAAAACAAAGGAGATGATTCAAGTTATTGAAGCTCCTGACATTGTCAAGAACAAAGTCTCCTTCTCCGTTTTCGGGTTCTTGGATGGAGAAGTCTCTTTAACAGGAAAGTTGAAGGCTCTGGATGATAAGTGGATCCAAGTTATATTTGAGCCCCCAGAACTGAAGGTTGGAGCCTTGGATTTCAGATACGGAGGTGAAAGCGAGGTTAAACTCCAGATTACATACATAGATGACAAGGTCAGGTTGGGGAAGGGCTCTAAAGGCTCTCTATTTGTTTTCCAGAGACTCGGTAGTTCCACCTGA

Protein Analysis

304

Amino Acids

34.44

Weight (kDa)

9.54

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 136 - 296 2e-12 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 78, 219, 597
AciI CCGC 1 cut(s) 169
AclWI GGATC 4 cut(s) 219, 345, 736, 749
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 4 cut(s) 4, 240, 269, 415
AcuI CTGAAG 3 cut(s) 194, 495, 791
AcyI GRCGYC 2 cut(s) 220, 598
AfaI GTAC 4 cut(s) 365, 406, 548, 589
AfiI CCNNNNNNNGG 3 cut(s) 406, 575, 681
AgeI ACCGGT 1 cut(s) 366
AgsI TTSAA 3 cut(s) 629, 638, 721
AjnI CCWGG 1 cut(s) 353
AjuI GAANNNNNNNTTGG 2 cut(s) 759, 791
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 6 cut(s) 111, 147, 154, 380, 464, 641
AluI AGCT 6 cut(s) 111, 147, 154, 380, 464, 641
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 5 cut(s) 350, 525, 670, 706, 886
AlwI GGATC 4 cut(s) 219, 345, 736, 749
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 3 cut(s) 48, 166, 331
ApeKI GCWGC 1 cut(s) 171
ApoI RAATTY 4 cut(s) 4, 240, 269, 415
AsiGI ACCGGT 1 cut(s) 366
AspLEI GCGC 3 cut(s) 222, 313, 600
AspS9I GGNCC 2 cut(s) 49, 369
AsuC2I CCSGG 2 cut(s) 570, 571
AsuHPI GGTGA 6 cut(s) 25, 197, 280, 421, 558, 815
AsuII TTCGAA 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 1 cut(s) 369
BaeI ACNNNNGTAYC 2 cut(s) 787, 820
BamHI GGATCC 1 cut(s) 741
BanI GGYRCC 3 cut(s) 78, 219, 597
BanII GRGCYC 3 cut(s) 382, 762, 866
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 1 cut(s) 158
BccI CCATC 3 cut(s) 61, 99, 686
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 355
BcnI CCSGG 2 cut(s) 570, 571
BcoDI GTCTC 5 cut(s) 350, 525, 670, 706, 886
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 580
BfoI RGCGCY 2 cut(s) 223, 601
BglI GCCNNNNNGGC 1 cut(s) 76
BisI GCNGC 2 cut(s) 169, 172
BlsI GCNGC 2 cut(s) 170, 173
Bme1390I CCNGG 3 cut(s) 355, 570, 571
Bme18I GGWCC 1 cut(s) 369
BmeT110I CYCGRG 1 cut(s) 569
BmgT120I GGNCC 2 cut(s) 49, 369
BmiI GGNNCC 7 cut(s) 51, 80, 221, 371, 599, 743, 781
BmrFI CCNGG 3 cut(s) 355, 570, 571
BmrI ACTGGG 1 cut(s) 544
BmuI ACTGGG 1 cut(s) 544
BpmI CTGGAG 2 cut(s) 376, 806
Bpu10I CCTNAGC 1 cut(s) 465
Bpu14I TTCGAA 1 cut(s) 142
BpuMI CCSGG 2 cut(s) 570, 571
BsaAI YACGTR 1 cut(s) 75
BsaHI GRCGYC 2 cut(s) 220, 598
BsaJI CCNNGG 5 cut(s) 163, 400, 569, 570, 783
BsaWI WCCGGW 1 cut(s) 366
BsaXI ACNNNNNCTCC 2 cut(s) 650, 680
Bsc4I CCNNNNNNNGG 3 cut(s) 406, 575, 681
Bse118I RCCGGY 2 cut(s) 46, 366
Bse1I ACTGG 2 cut(s) 506, 539
Bse3DI GCAATG 1 cut(s) 65
BseBI CCWGG 1 cut(s) 355
BseDI CCNNGG 5 cut(s) 163, 400, 569, 570, 783
BseGI GGATG 3 cut(s) 544, 697, 736
BseLI CCNNNNNNNGG 3 cut(s) 406, 575, 681
BseMI GCAATG 1 cut(s) 65
BseMII CTCAG 2 cut(s) 162, 567
BseNI ACTGG 2 cut(s) 506, 539
BseRI GAGGAG 2 cut(s) 229, 441
BseXI GCAGC 1 cut(s) 158
BshFI GGCC 3 cut(s) 50, 168, 333
BshNI GGYRCC 3 cut(s) 78, 219, 597
BshTI ACCGGT 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 3 cut(s) 47, 367, 570
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 3 cut(s) 406, 575, 681
BsmAI GTCTC 5 cut(s) 350, 525, 670, 706, 886
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 355
BsnI GGCC 3 cut(s) 50, 168, 333
BsoBI CYCGRG 1 cut(s) 569
Bsp119I TTCGAA 1 cut(s) 142
Bsp1286I GDGCHC 3 cut(s) 382, 762, 866
Bsp143I GATC 3 cut(s) 224, 350, 741
Bsp19I CCATGG 1 cut(s) 163
BspACI CCGC 1 cut(s) 169
BspANI GGCC 3 cut(s) 50, 168, 333
BspCNI CTCAG 2 cut(s) 161, 566
BspLI GGNNCC 7 cut(s) 51, 80, 221, 371, 599, 743, 781
BspPI GGATC 4 cut(s) 219, 345, 736, 749
BspQI GCTCTTC 1 cut(s) 370
BspT104I TTCGAA 1 cut(s) 142
BspT107I GGYRCC 3 cut(s) 78, 219, 597
BsrDI GCAATG 1 cut(s) 65
BsrFI RCCGGY 2 cut(s) 46, 366
BsrI ACTGG 2 cut(s) 506, 539
BssAI RCCGGY 2 cut(s) 46, 366
BssECI CCNNGG 5 cut(s) 163, 400, 569, 570, 783
BssMI GATC 3 cut(s) 224, 350, 741
BssNI GRCGYC 2 cut(s) 220, 598
BssT1I CCWWGG 3 cut(s) 163, 400, 783
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 4 cut(s) 284, 303, 409, 494
Bst6I CTCTTC 1 cut(s) 370
BstACI GRCGYC 2 cut(s) 220, 598
BstBAI YACGTR 1 cut(s) 75
BstBI TTCGAA 1 cut(s) 142
BstDEI CTNAG 3 cut(s) 148, 465, 553
BstDSI CCRYGG 1 cut(s) 163
BstF5I GGATG 3 cut(s) 544, 697, 736
BstH2I RGCGCY 2 cut(s) 223, 601
BstHHI GCGC 3 cut(s) 222, 313, 600
BstKTI GATC 3 cut(s) 227, 353, 744
BstMAI GTCTC 5 cut(s) 350, 525, 670, 706, 886
BstMBI GATC 3 cut(s) 224, 350, 741
BstMWI GCNNNNNNNGC 5 cut(s) 76, 177, 198, 470, 870
BstNI CCWGG 1 cut(s) 355
BstSCI CCNGG 3 cut(s) 353, 568, 569
BstSFI CTRYAG 1 cut(s) 580
BstV1I GCAGC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 741
BstXI CCANNNNNNTGG 1 cut(s) 272
BstYI RGATCY 1 cut(s) 741
BsuRI GGCC 3 cut(s) 50, 168, 333
BtgI CCRYGG 1 cut(s) 163
BtsCI GGATG 3 cut(s) 544, 697, 736
BtsIMutI CAGTG 2 cut(s) 513, 561
CfoI GCGC 3 cut(s) 222, 313, 600
Cfr10I RCCGGY 2 cut(s) 46, 366
Cfr13I GGNCC 2 cut(s) 49, 369
Cfr9I CCCGGG 1 cut(s) 569
CseI GACGC 1 cut(s) 376
Csp6I GTAC 4 cut(s) 364, 405, 547, 588
CspAI ACCGGT 1 cut(s) 366
CviAII CATG 1 cut(s) 164
CviQI GTAC 4 cut(s) 364, 405, 547, 588
DdeI CTNAG 3 cut(s) 148, 465, 553
DinI GGCGCC 2 cut(s) 221, 599
DpnI GATC 3 cut(s) 226, 352, 743
DpnII GATC 3 cut(s) 224, 350, 741
DraI TTTAAA 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 370
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 3 cut(s) 163, 400, 783
Eco24I GRGCYC 3 cut(s) 382, 762, 866
Eco47I GGWCC 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 3 cut(s) 194, 495, 791
Eco88I CYCGRG 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 380
EcoRI GAATTC 2 cut(s) 4, 415
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 3 cut(s) 163, 400, 783
EcoT38I GRGCYC 3 cut(s) 382, 762, 866
EgeI GGCGCC 2 cut(s) 221, 599
EheI GGCGCC 2 cut(s) 221, 599
ErhI CCWWGG 3 cut(s) 163, 400, 783
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 1 cut(s) 167
FaiI YATR 4 cut(s) 165, 752, 832, 836
FaqI GGGAC 1 cut(s) 355
FatI CATG 1 cut(s) 163
Fnu4HI GCNGC 2 cut(s) 169, 172
FokI GGATG 3 cut(s) 551, 704, 743
FriOI GRGCYC 3 cut(s) 382, 762, 866
Fsp4HI GCNGC 2 cut(s) 169, 172
FspBI CTAG 1 cut(s) 189
GlaI GCGC 3 cut(s) 221, 312, 599
GluI GCNGC 2 cut(s) 169, 172
GsuI CTGGAG 2 cut(s) 376, 806
HaeII RGCGCY 2 cut(s) 223, 601
HaeIII GGCC 3 cut(s) 50, 168, 333
HapII CCGG 3 cut(s) 47, 367, 570
HgaI GACGC 1 cut(s) 376
HhaI GCGC 3 cut(s) 222, 313, 600
Hin1I GRCGYC 2 cut(s) 220, 598
Hin1II CATG 1 cut(s) 167
Hin6I GCGC 3 cut(s) 220, 311, 598
HinP1I GCGC 3 cut(s) 220, 311, 598
HinfI GANTC 3 cut(s) 60, 625, 894
HpaII CCGG 3 cut(s) 47, 367, 570
HphI GGTGA 6 cut(s) 25, 197, 280, 421, 558, 815
Hpy166II GTNNAC 1 cut(s) 547
Hpy188I TCNGA 7 cut(s) 99, 151, 213, 350, 421, 530, 794
Hpy188III TCNNGA 7 cut(s) 57, 372, 644, 655, 728, 823, 889
Hpy8I GTNNAC 1 cut(s) 547
HpyAV CCTTC 8 cut(s) 10, 169, 212, 218, 679, 715, 766, 853
HpyCH4III ACNGT 4 cut(s) 284, 303, 409, 494
HpyCH4IV ACGT 2 cut(s) 74, 590
HpyCH4V TGCA 1 cut(s) 484
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 177, 198, 470, 870
HpyF3I CTNAG 3 cut(s) 148, 465, 553
HpySE526I ACGT 2 cut(s) 74, 590
Hsp92I GRCGYC 2 cut(s) 220, 598
Hsp92II CATG 1 cut(s) 167
HspAI GCGC 3 cut(s) 220, 311, 598
KasI GGCGCC 2 cut(s) 219, 597
Kzo9I GATC 3 cut(s) 224, 350, 741
LguI GCTCTTC 1 cut(s) 370
LmnI GCTCC 2 cut(s) 646, 779
Lsp1109I GCAGC 1 cut(s) 158
MaeI CTAG 1 cut(s) 189
MaeII ACGT 2 cut(s) 74, 590
MaeIII GTNAC 1 cut(s) 409
MalI GATC 3 cut(s) 226, 352, 743
MboI GATC 3 cut(s) 224, 350, 741
MboII GAAGA 2 cut(s) 387, 488
MflI RGATCY 1 cut(s) 741
MhlI GDGCHC 3 cut(s) 382, 762, 866
MluCI AATT 5 cut(s) 4, 240, 269, 415, 479
Mly113I GGCGCC 2 cut(s) 220, 598
MlyI GAGTC 1 cut(s) 888
MmeI TCCRAC 1 cut(s) 757
MseI TTAA 4 cut(s) 12, 609, 707, 816
MspA1I CMGCKG 1 cut(s) 171
MspI CCGG 3 cut(s) 47, 367, 570
MspR9I CCNGG 3 cut(s) 355, 570, 571
MvaI CCWGG 1 cut(s) 355
MwoI GCNNNNNNNGC 5 cut(s) 76, 177, 198, 470, 870
NarI GGCGCC 2 cut(s) 220, 598
NciI CCSGG 2 cut(s) 570, 571
NcoI CCATGG 1 cut(s) 163
NdeII GATC 3 cut(s) 224, 350, 741
NlaIII CATG 1 cut(s) 167
NlaIV GGNNCC 7 cut(s) 51, 80, 221, 371, 599, 743, 781
NmuCI GTSAC 1 cut(s) 409
NspV TTCGAA 1 cut(s) 142
PciSI GCTCTTC 1 cut(s) 370
PfeI GAWTC 2 cut(s) 60, 625
PflFI GACNNNGTC 2 cut(s) 650, 845
PfoI TCCNGGA 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 366
PkrI GCNGC 2 cut(s) 170, 173
PleI GAGTC 1 cut(s) 888
PluTI GGCGCC 2 cut(s) 223, 601
PpsI GAGTC 1 cut(s) 888
Ppu21I YACGTR 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 7 cut(s) 51, 80, 221, 371, 599, 743, 781
PspPI GGNCC 2 cut(s) 49, 369
PsuI RGATCY 1 cut(s) 741
PsyI GACNNNGTC 2 cut(s) 650, 845
RsaI GTAC 4 cut(s) 365, 406, 548, 589
RsaNI GTAC 4 cut(s) 364, 405, 547, 588
SacI GAGCTC 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 370
SaqAI TTAA 4 cut(s) 12, 609, 707, 816
SatI GCNGC 2 cut(s) 169, 172
Sau3AI GATC 3 cut(s) 224, 350, 741
Sau96I GGNCC 2 cut(s) 49, 369
SchI GAGTC 1 cut(s) 888
ScrFI CCNGG 3 cut(s) 355, 570, 571
SduI GDGCHC 3 cut(s) 382, 762, 866
SfcI CTRYAG 1 cut(s) 580
SfoI GGCGCC 2 cut(s) 221, 599
SfuI TTCGAA 1 cut(s) 142
SinI GGWCC 1 cut(s) 369
SmaI CCCGGG 1 cut(s) 571
Sse9I AATT 5 cut(s) 4, 240, 269, 415, 479
SsiI CCGC 1 cut(s) 169
SspDI GGCGCC 2 cut(s) 219, 597
SspI AATATT 1 cut(s) 328
SspMI CTAG 1 cut(s) 189
SstI GAGCTC 1 cut(s) 382
StyD4I CCNGG 3 cut(s) 353, 568, 569
StyI CCWWGG 3 cut(s) 163, 400, 783
TaaI ACNGT 4 cut(s) 284, 303, 409, 494
TaiI ACGT 2 cut(s) 77, 593
TaqI TCGA 3 cut(s) 58, 142, 231
TasI AATT 5 cut(s) 4, 240, 269, 415, 479
TatI WGTACW 1 cut(s) 546
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 2 cut(s) 60, 625
Tru1I TTAA 4 cut(s) 12, 609, 707, 816
Tru9I TTAA 4 cut(s) 12, 609, 707, 816
TscAI CASTG 2 cut(s) 513, 561
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 171
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 332, 664, 813
TspMI CCCGGG 1 cut(s) 569
TspRI CASTG 2 cut(s) 513, 561
Tth111I GACNNNGTC 2 cut(s) 650, 845
VpaK11BI GGWCC 1 cut(s) 369
XapI RAATTY 4 cut(s) 4, 240, 269, 415
XmaI CCCGGG 1 cut(s) 569
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.