Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0008122

Physical Location & Seq

Genomic Coordinates Reverse (-)

7335071 .. 7339782

Gene Structure 4712 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2847 bp

ATGATAATGTCCAACCCATTAAGACCAAAAAATGTAAAAACTCACATTTTCTCTCAGACTTTCACATTTTCTCACCAGAGGTTAGTAAAGTGGGAAAAATGCAGGCGGAGAATTGTAGGGTTAGGGTTTCGATTAAGCTTCCGAGTTCACACCATGGATTCACCTGAAATTCCCAAAATCAAAGCCAACACTTCACCTTCCTCAGATTCTCCTCCAGTTCAAGAGTCACCAGTCTTCAGTTACATAAGCAATTTATCACCAATACAGCCTGTGAAGGCTTCACATGCAGTGCAAGGGTTCCCGGGACTTAGCTCTCCTCCTCTTGTGTTCACGTCACCACGCATAAATACACTCCGGGAAACAAGTTTCCTGAAAAGGCCTCAATACCCGCAATTATCCAGTGTAGGAAAAACGAAAGCACATGATGAAGCCAAAACGTTTGTTGATGTTCCAGTTGATTCCAAGAAACATATCACCCAACTGCAGATGGGATTGATTACTGATACTCAGGACTGTGAGACCAACAGTTCCTCGGAGGGTGTCGATGAATACTTAGCTGATCCCATGGAGATGGACTGTACGAATTCTTGTCAATCAGTCAATCCATGTTTGAAAGAATCTATCAATGCATCTGAATCTGAGGCACCACCAGAGAAGGCTAAAGAGGACCTTGATGGAAAGCAATTTGATCCAAAACCTGTGAAAAATAAAGTGCATAGTGATGGTGAGTTGCCATCTTCTGAGTGCCCAAAGGTTGGATCCGGCTTATCCATCGATAATGCTTTCAATGGGGAGCATCACCTAGGTTTTCATAATCAGGGTATTGGAGGTAGACAGCAGGATGATTGTGATCATAACCCTCCATCTCCTCCTGGACGTTTGCAGATTGGTCAGGTGTATGAGGATTGTACTGAGAAGGTTGGTGGAACCTCAAAGGGAATGATTGGAAACATGATATTGCATGCTCCTAATAAGGCCAAAAATGACCAAGGTGGCATGCATAGACGTTGCCTTCAGTTTGAAGAGGCACCTCCTTGTGCCACTGGAAAAGGAGACAGTTCTTCTGTAGAGAAAGTTAACAATTCAGAACCACCTGCCAGCACAGCAGAATTGGAGATTGTGCAAGTACCTAATGCAGCAACTTCCAAGAGACAGATGGGAGCCTCATTGCCACCTCGGTATGGTGGAACCTCTCCTTTAACTGTTCCCAAACCATCAGGTATTGGCTTGCATCTAAATAGCATAGTCAATGCTGCACCTGTTGTTCGTGGTGCAACAACGATAAAATTAGCAGATCACTACATTGGTGTGCAAGTTATGAAATCATCATCTGTTGTGAACCACCATTTACCTGAGAATGTGAGGGATGAAACTGAGAATTCAATTGCCGCAAGCTCCTCCATCACCCAGTCTCCCGATACTGTGGAATTTGAGCGTCATGGATCTCCACCCGAGAAGAGCAAAGTAGATTCTCAGAATGTTGATAGTTATATGGAGTTGAACCAATCTAGCTCTCAAAAGAAAAGGAAAAAAACACCAAGCTCTAAAGATAGTGATGGGTGCAAGCGGTGCAACTGTAAGAAGTCCAAGTGCTTGAAACTTTATTGCGATTGTTTTGCTGCTGGAGTCTATTGTGCCGAAACTTGTGCTTGCCAAGGATGCTTTAATATACCTGATTATGAAGATACGGTTCTTGAGACGAGACAACAAATTGAAGCCCGTAATCCACTTGCATTTGCTCCCAAGATTGTACAGCTAGAAGAAGAAATTCAATTTACACCAGCCTCAGCAAGACACAAAAGGGGATGCAATTGCAAAAAGTCAATGTGTCTGAAAAAATATTGTGAATGCTATCAGGCCAATGTTGGATGCTCCAGTGGATGCCGGTGTGATGGATGTAAAAATGTTTATGGCAGAAAGGGAGATTTTGTTCCAATAGAGCATGGTGTGATTAAGGACACGATCAGTGACAAAGCCGGTAAGGAAACATTTGATGAGAAGCTGGAAATGGTGGCAACAAAGAAAGATATTTTGTCTACTGATTTGTATGATTCACACAACCACACTCCATTGACGCCATCATTTCACTGCTCAGATCATGCAAATAACATACCAAAATCCCCGTGCCTTCCTACAAGTTACCTTCGATCACCTGAATCTGATCTTACCATCATATCATCTTATGAAAAATCAACAAGATCCCCTCTGAGAAATTCAAAGACAGGTGGCATTCTTCTGGAGACAAGTAAAGAATTATCGAATATGGGTTATTACCACTGGCGAGAGGACTATGACAACATTGGAGTAGCGGATACTTTTTCAACAAGATACGATGCTGCCCCCACTACATGCCATATTACTCCATTATCAGATCTTTACTCAAAGGCCTCTGCTTCTTCCACATCATCAAGAAGTGATTGGAAAAATGCTTCACAAGCTCAGCTGTGTCCTGGAAGTCAAGGTCTGTCATCTAGTAGTTCTCTTCATTGGTGCAGTTCACCAGTCACCCCAATGGCTCGGTTAGGTGGGACGAAATCTTCTCAGGGTCTTGACTTTGACAATGGGCTTTATGACTTTCTGCAAGAAGAAACGCCTGAGATACTGAAGGACAGTTCTAATCCTATTAGGTCTGTGAAAGTTAGTTCCCCCAATAAGAAACGGGTTTCTCCTCCTCACAACCACAATCATGAGCTTGGTGCAAGCTCTTCAGGGAGCTTGAGAAGTGGCCGCAAGTTCATATTGAAGGCTGTCCCTTCGTTCCCACCCCTTACCCCTTGTAGCAATTCCGAAGGTAGTAATATCATTCAGAACACTAGTAACCTTCACAATAAGGGCAGAAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04218 map04218

Pfam Domains

Protein Families

TCR

Protein Analysis

949

Amino Acids

103.59

Weight (kDa)

8.2

Isoelectric Point (pI)

62.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TCR	PF03638	522 - 556	2.3e-11	Tesmin/TSO1-like CXC domain, cysteine-rich domain
TCR	PF03638	600 - 635	3.9e-11	Tesmin/TSO1-like CXC domain, cysteine-rich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008122)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g29570 FvH4_2g29570 FvH4_2g29570
malus_domestica	MD08G1145800.v1.1 MD15G1121200.v1.1
prunus_persica	Prupe.1G473800_v2.0.a1 Prupe.1G473800_v2.0.a1 Prupe.1G473800_v2.0.a1
pyrus_communis	pycom08g12270 pycom15g10940
rosa_chinensis	RchiOBHm_Chr6g0298531
rosa_multiflora	Rmu_co8315197.1_g000001 Rmu_co8448729.1_g000001 Rmu_sc0004500.1_g000005 Rmu_ssc0000289.1_g000056
rosa_roxburghii	Rroxscaffold_7G00169560
rosa_rugosa	Rorug06G0281000 Rorug06G0281000
rosa_samantha	Rh6AG392300 Rh6BG400100 Rh6CG406000 Rh6DG392400 Rh6DG392500
rosa_wichuraiana	Rw6G034290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1102
Acc36I	ACCTGC	1 cut(s)	1102
AccB1I	GGYRCC	2 cut(s)	643, 1027
AccB7I	CCANNNNNTGG	1 cut(s)	755
AccI	GTMKAC	2 cut(s)	832, 2036
AciI	CCGC	6 cut(s)	106, 389, 1389, 1567, 2309, 2728
AclI	AACGTT	1 cut(s)	437
AclWI	GGATC	6 cut(s)	554, 683, 753, 766, 1450, 2193
AcoI	YGGCCR	1 cut(s)	2725
AcsI	RAATTY	6 cut(s)	168, 583, 1378, 1427, 1767, 2212
AcuI	CTGAAG	4 cut(s)	220, 998, 2624, 2691
AcyI	GRCGYC	1 cut(s)	2075
AfaI	GTAC	4 cut(s)	580, 910, 1128, 1752
AfiI	CCNNNNNNNGG	2 cut(s)	755, 1181
AhlI	ACTAGT	1 cut(s)	2813
AjiI	CACGTC	1 cut(s)	333
AjnI	CCWGG	2 cut(s)	871, 2449
AloI	GAACNNNNNNTCC	2 cut(s)	1914, 1946
Alw26I	GTCTC	7 cut(s)	512, 1047, 1144, 1416, 1691, 1696, 2234
AlwI	GGATC	6 cut(s)	554, 683, 753, 766, 1450, 2193
Ama87I	CYCGRG	2 cut(s)	301, 1451
AoxI	GGCC	5 cut(s)	377, 975, 1857, 2385, 2725
ApeKI	GCWGC	4 cut(s)	1136, 1253, 1619, 2336
ApoI	RAATTY	6 cut(s)	168, 583, 1378, 1427, 1767, 2212
Asp700I	GAANNNNTTC	1 cut(s)	1767
AspA2I	CCTAGG	1 cut(s)	802
AspS9I	GGNCC	1 cut(s)	667
AsuC2I	CCSGG	3 cut(s)	302, 303, 356
AvaI	CYCGRG	2 cut(s)	301, 1451
AvaII	GGWCC	1 cut(s)	667
AvrII	CCTAGG	1 cut(s)	802
BaeGI	GKGCMC	1 cut(s)	749
BamHI	GGATCC	1 cut(s)	758
BanI	GGYRCC	2 cut(s)	643, 1027
BbsI	GAAGAC	1 cut(s)	226
BbvCI	CCTCAGC	1 cut(s)	1786
BbvI	GCAGC	4 cut(s)	1148, 1240, 1606, 2323
BcgI	CGANNNNNNTGC	4 cut(s)	1598, 1628, 1632, 1662
BciT130I	CCWGG	2 cut(s)	873, 2451
BciVI	GTATCC	1 cut(s)	2305
BclI	TGATCA	1 cut(s)	850
BcnI	CCSGG	3 cut(s)	302, 303, 356
BcoDI	GTCTC	7 cut(s)	512, 1047, 1144, 1416, 1691, 1696, 2234
BcuI	ACTAGT	1 cut(s)	2813
BfaI	CTAG	5 cut(s)	803, 1509, 1757, 2472, 2814
BfmI	CTRYAG	2 cut(s)	482, 1065
BfuAI	ACCTGC	1 cut(s)	1102
BfuI	GTATCC	1 cut(s)	2305
BglII	AGATCT	1 cut(s)	2371
BisI	GCNGC	6 cut(s)	1137, 1254, 1389, 1620, 2337, 2728
BlnI	CCTAGG	1 cut(s)	802
BlpI	GCTNAGC	1 cut(s)	2439
BlsI	GCNGC	6 cut(s)	1138, 1255, 1390, 1621, 2338, 2729
Bme1390I	CCNGG	5 cut(s)	302, 303, 356, 873, 2451
Bme18I	GGWCC	1 cut(s)	667
BmeT110I	CYCGRG	2 cut(s)	301, 1451
BmgBI	CACGTC	1 cut(s)	333
BmgT120I	GGNCC	1 cut(s)	667
BmiI	GGNNCC	7 cut(s)	299, 645, 760, 928, 1029, 1162, 1189
BmrFI	CCNGG	5 cut(s)	302, 303, 356, 873, 2451
BmrI	ACTGGG	1 cut(s)	1402
BmsI	GCATC	8 cut(s)	638, 805, 1240, 1649, 1796, 1859, 1871, 2323
BmuI	ACTGGG	1 cut(s)	1402
BpiI	GAAGAC	1 cut(s)	226
BpmI	CTGGAG	4 cut(s)	198, 1644, 1858, 2258
Bpu10I	CCTNAGC	1 cut(s)	1786
Bpu1102I	GCTNAGC	1 cut(s)	2439
BpuEI	CTTGAG	2 cut(s)	1715, 2737
BpuMI	CCSGG	3 cut(s)	302, 303, 356
Bsa29I	ATCGAT	1 cut(s)	774
BsaBI	GATNNNNATC	1 cut(s)	849
BsaHI	GRCGYC	1 cut(s)	2075
BsaI	GGTCTC	1 cut(s)	512
BsaJI	CCNNGG	8 cut(s)	153, 301, 531, 564, 802, 988, 1175, 1654
BsaXI	ACNNNNNCTCC	4 cut(s)	1396, 1426, 1914, 1944
Bsc4I	CCNNNNNNNGG	2 cut(s)	755, 1181
Bse118I	RCCGGY	2 cut(s)	1884, 1976
Bse1I	ACTGG	9 cut(s)	215, 230, 399, 452, 1048, 1408, 1875, 2282, 2501
Bse3DI	GCAATG	1 cut(s)	1166
Bse8I	GATNNNNATC	1 cut(s)	849
BseBI	CCWGG	2 cut(s)	873, 2451
BseCI	ATCGAT	1 cut(s)	774
BseDI	CCNNGG	8 cut(s)	153, 301, 531, 564, 802, 988, 1175, 1654
BseGI	GGATG	7 cut(s)	847, 1372, 1664, 1811, 1874, 1886, 1901
BseJI	GATNNNNATC	1 cut(s)	849
BseLI	CCNNNNNNNGG	2 cut(s)	755, 1181
BseMI	GCAATG	1 cut(s)	1166
BseNI	ACTGG	9 cut(s)	215, 230, 399, 452, 1048, 1408, 1875, 2282, 2501
BseRI	GAGGAG	7 cut(s)	201, 306, 309, 858, 1387, 2658, 2661
BseSI	GKGCMC	1 cut(s)	749
BseXI	GCAGC	4 cut(s)	1148, 1240, 1606, 2323
BsgI	GTGCAG	2 cut(s)	1239, 2512
BshFI	GGCC	5 cut(s)	379, 977, 1859, 2387, 2727
BshNI	GGYRCC	2 cut(s)	643, 1027
BshVI	ATCGAT	1 cut(s)	774
BsiHKCI	CYCGRG	2 cut(s)	301, 1451
BsiSI	CCGG	5 cut(s)	302, 355, 762, 1885, 1977
BslFI	GGGAC	3 cut(s)	318, 2542, 2735
BslI	CCNNNNNNNGG	2 cut(s)	755, 1181
BsmAI	GTCTC	7 cut(s)	512, 1047, 1144, 1416, 1691, 1696, 2234
BsmBI	CGTCTC	1 cut(s)	1691
BsmFI	GGGAC	3 cut(s)	318, 2542, 2735
BsmI	GAATGC	2 cut(s)	1853, 2229
BsnI	GGCC	5 cut(s)	379, 977, 1859, 2387, 2727
Bso31I	GGTCTC	1 cut(s)	512
BsoBI	CYCGRG	2 cut(s)	301, 1451
Bsp1286I	GDGCHC	1 cut(s)	749
Bsp1407I	TGTACA	1 cut(s)	1750
Bsp1720I	GCTNAGC	1 cut(s)	2439
Bsp19I	CCATGG	2 cut(s)	153, 564
BspACI	CCGC	6 cut(s)	106, 389, 1389, 1567, 2309, 2728
BspANI	GGCC	5 cut(s)	379, 977, 1859, 2387, 2727
BspDI	ATCGAT	1 cut(s)	774
BspHI	TCATGA	1 cut(s)	2686
BspLI	GGNNCC	7 cut(s)	299, 645, 760, 928, 1029, 1162, 1189
BspMAI	CTGCAG	1 cut(s)	486
BspMI	ACCTGC	1 cut(s)	1102
BspPI	GGATC	6 cut(s)	554, 683, 753, 766, 1450, 2193
BspQI	GCTCTTC	2 cut(s)	1451, 2710
BspT107I	GGYRCC	2 cut(s)	643, 1027
BspTNI	GGTCTC	1 cut(s)	512
BsrDI	GCAATG	1 cut(s)	1166
BsrFI	RCCGGY	2 cut(s)	1884, 1976
BsrGI	TGTACA	1 cut(s)	1750
BsrI	ACTGG	9 cut(s)	215, 230, 399, 452, 1048, 1408, 1875, 2282, 2501
BssAI	RCCGGY	2 cut(s)	1884, 1976
BssECI	CCNNGG	8 cut(s)	153, 301, 531, 564, 802, 988, 1175, 1654
BssNI	GRCGYC	1 cut(s)	2075
BssT1I	CCWWGG	5 cut(s)	153, 564, 802, 988, 1654
Bst2UI	CCWGG	2 cut(s)	873, 2451
Bst4CI	ACNGT	9 cut(s)	515, 527, 578, 1058, 1204, 1423, 1577, 1690, 2612
Bst6I	CTCTTC	4 cut(s)	1017, 1451, 2487, 2710
BstACI	GRCGYC	1 cut(s)	2075
BstAPI	GCANNNNNTGC	1 cut(s)	1569
BstAUI	TGTACA	1 cut(s)	1750
BstC8I	GCNNGC	9 cut(s)	104, 963, 998, 1099, 1229, 1393, 1565, 1651, 2701
BstDSI	CCRYGG	2 cut(s)	153, 564
BstF5I	GGATG	7 cut(s)	847, 1372, 1664, 1811, 1874, 1886, 1901
BstMAI	GTCTC	7 cut(s)	512, 1047, 1144, 1416, 1691, 1696, 2234
BstMWI	GCNNNNNNNGC	5 cut(s)	284, 1103, 1569, 1659, 2435
BstNI	CCWGG	2 cut(s)	873, 2451
BstNSI	RCATGY	4 cut(s)	287, 965, 1000, 2352
BstSCI	CCNGG	5 cut(s)	300, 301, 354, 871, 2449
BstSFI	CTRYAG	2 cut(s)	482, 1065
BstSLI	GKGCMC	1 cut(s)	749
BstV1I	GCAGC	4 cut(s)	1148, 1240, 1606, 2323
BstV2I	GAAGAC	1 cut(s)	226
BstX2I	RGATCY	4 cut(s)	758, 1442, 2198, 2371
BstXI	CCANNNNNNTGG	1 cut(s)	571
BstYI	RGATCY	4 cut(s)	758, 1442, 2198, 2371
Bsu15I	ATCGAT	1 cut(s)	774
BsuI	GTATCC	1 cut(s)	2305
BsuRI	GGCC	5 cut(s)	379, 977, 1859, 2387, 2727
BsuTUI	ATCGAT	1 cut(s)	774
BtgI	CCRYGG	2 cut(s)	153, 564
BtrI	CACGTC	1 cut(s)	333
BtsCI	GGATG	7 cut(s)	847, 1372, 1664, 1811, 1874, 1886, 1901
BtsI	GCAGTG	2 cut(s)	294, 2086
BtsIMutI	CAGTG	7 cut(s)	294, 406, 1041, 1882, 1972, 2086, 2275
BveI	ACCTGC	1 cut(s)	1102
Cac8I	GCNNGC	9 cut(s)	104, 963, 998, 1099, 1229, 1393, 1565, 1651, 2701
CciI	TCATGA	1 cut(s)	2686
Cfr10I	RCCGGY	2 cut(s)	1884, 1976
Cfr13I	GGNCC	1 cut(s)	667
Cfr9I	CCCGGG	1 cut(s)	301
ClaI	ATCGAT	1 cut(s)	774
CseI	GACGC	2 cut(s)	1424, 2083
Csp6I	GTAC	4 cut(s)	579, 909, 1127, 1751
CviQI	GTAC	4 cut(s)	579, 909, 1127, 1751
EaeI	YGGCCR	1 cut(s)	2725
Eam1104I	CTCTTC	4 cut(s)	1017, 1451, 2487, 2710
EarI	CTCTTC	4 cut(s)	1017, 1451, 2487, 2710
EciI	GGCGGA	1 cut(s)	121
Eco130I	CCWWGG	5 cut(s)	153, 564, 802, 988, 1654
Eco147I	AGGCCT	2 cut(s)	379, 2387
Eco31I	GGTCTC	1 cut(s)	512
Eco47I	GGWCC	1 cut(s)	667
Eco57I	CTGAAG	4 cut(s)	220, 998, 2624, 2691
Eco88I	CYCGRG	2 cut(s)	301, 1451
EcoO109I	RGGNCCY	1 cut(s)	667
EcoRI	GAATTC	2 cut(s)	583, 1378
EcoRII	CCWGG	2 cut(s)	871, 2449
EcoT14I	CCWWGG	5 cut(s)	153, 564, 802, 988, 1654
EcoT22I	ATGCAT	2 cut(s)	631, 1002
ErhI	CCWWGG	5 cut(s)	153, 564, 802, 988, 1654
Esp3I	CGTCTC	1 cut(s)	1691
FalI	AAGNNNNNCTT	2 cut(s)	654, 686
FaqI	GGGAC	3 cut(s)	318, 2542, 2735
FauI	CCCGC	1 cut(s)	396
FbaI	TGATCA	1 cut(s)	850
FblI	GTMKAC	2 cut(s)	832, 2036
Fnu4HI	GCNGC	6 cut(s)	1137, 1254, 1389, 1620, 2337, 2728
FokI	GGATG	7 cut(s)	854, 1379, 1671, 1818, 1881, 1893, 1908
Fsp4HI	GCNGC	6 cut(s)	1137, 1254, 1389, 1620, 2337, 2728
FspBI	CTAG	5 cut(s)	803, 1509, 1757, 2472, 2814
GluI	GCNGC	6 cut(s)	1137, 1254, 1389, 1620, 2337, 2728
GsuI	CTGGAG	4 cut(s)	198, 1644, 1858, 2258
HaeIII	GGCC	5 cut(s)	379, 977, 1859, 2387, 2727
HapII	CCGG	5 cut(s)	302, 355, 762, 1885, 1977
HgaI	GACGC	2 cut(s)	1424, 2083
Hin1I	GRCGYC	1 cut(s)	2075
HincII	GTYRAC	1 cut(s)	1078
HindII	GTYRAC	1 cut(s)	1078
HindIII	AAGCTT	1 cut(s)	136
HpaI	GTTAAC	1 cut(s)	1078
HpaII	CCGG	5 cut(s)	302, 355, 762, 1885, 1977
Hpy166II	GTNNAC	7 cut(s)	148, 330, 833, 1078, 1339, 2037, 2498
Hpy188III	TCNNGA	9 cut(s)	221, 370, 509, 1415, 1694, 2237, 2409, 2549, 2687
Hpy8I	GTNNAC	7 cut(s)	148, 330, 833, 1078, 1339, 2037, 2498
HpyCH4III	ACNGT	9 cut(s)	515, 527, 578, 1058, 1204, 1423, 1577, 1690, 2612
HpyCH4IV	ACGT	4 cut(s)	332, 437, 877, 1006
HpyF10VI	GCNNNNNNNGC	5 cut(s)	284, 1103, 1569, 1659, 2435
HpySE526I	ACGT	4 cut(s)	332, 437, 877, 1006
Hsp92I	GRCGYC	1 cut(s)	2075
Ksp22I	TGATCA	1 cut(s)	850
KspAI	GTTAAC	1 cut(s)	1078
LguI	GCTCTTC	2 cut(s)	1451, 2710
LmnI	GCTCC	7 cut(s)	793, 970, 1160, 1400, 1744, 1877, 2712
Lsp1109I	GCAGC	4 cut(s)	1148, 1240, 1606, 2323
LweI	GCATC	8 cut(s)	638, 805, 1240, 1649, 1796, 1859, 1871, 2323
MaeI	CTAG	5 cut(s)	803, 1509, 1757, 2472, 2814
MaeII	ACGT	4 cut(s)	332, 437, 877, 1006
MaeIII	GTNAC	7 cut(s)	225, 239, 333, 1967, 2138, 2503, 2816
MfeI	CAATTG	2 cut(s)	1383, 1810
MflI	RGATCY	4 cut(s)	758, 1442, 2198, 2371
MhlI	GDGCHC	1 cut(s)	749
MlyI	GAGTC	2 cut(s)	233, 1635
MmeI	TCCRAC	3 cut(s)	36, 736, 1846
Mph1103I	ATGCAT	2 cut(s)	631, 1002
MroXI	GAANNNNTTC	1 cut(s)	1767
MseI	TTAA	6 cut(s)	20, 134, 1077, 1199, 1665, 1953
MslI	CAYNNNNRTG	5 cut(s)	569, 720, 1307, 2510, 2685
MspA1I	CMGCKG	1 cut(s)	2443
MspI	CCGG	5 cut(s)	302, 355, 762, 1885, 1977
MspR9I	CCNGG	5 cut(s)	302, 303, 356, 873, 2451
MunI	CAATTG	2 cut(s)	1383, 1810
Mva1269I	GAATGC	2 cut(s)	1853, 2229
MvaI	CCWGG	2 cut(s)	873, 2451
MwoI	GCNNNNNNNGC	5 cut(s)	284, 1103, 1569, 1659, 2435
NciI	CCSGG	3 cut(s)	302, 303, 356
NcoI	CCATGG	2 cut(s)	153, 564
NlaIV	GGNNCC	7 cut(s)	299, 645, 760, 928, 1029, 1162, 1189
NmuCI	GTSAC	4 cut(s)	225, 333, 1967, 2503
NsiI	ATGCAT	2 cut(s)	631, 1002
NspI	RCATGY	4 cut(s)	287, 965, 1000, 2352
PaeI	GCATGC	2 cut(s)	965, 1000
PagI	TCATGA	1 cut(s)	2686
PaqCI	CACCTGC	1 cut(s)	1102
PceI	AGGCCT	2 cut(s)	379, 2387
PciSI	GCTCTTC	2 cut(s)	1451, 2710
PctI	GAATGC	2 cut(s)	1853, 2229
PdmI	GAANNNNTTC	1 cut(s)	1767
PfeI	GAWTC	8 cut(s)	158, 206, 458, 617, 635, 1469, 2051, 2156
PflMI	CCANNNNNTGG	1 cut(s)	755
PfoI	TCCNGGA	3 cut(s)	354, 871, 2449
PkrI	GCNGC	6 cut(s)	1138, 1255, 1390, 1621, 2338, 2729
PleI	GAGTC	2 cut(s)	232, 1634
PpsI	GAGTC	2 cut(s)	232, 1634
PpuMI	RGGWCCY	1 cut(s)	667
Psp1406I	AACGTT	1 cut(s)	437
Psp5II	RGGWCCY	1 cut(s)	667
Psp6I	CCWGG	2 cut(s)	871, 2449
PspGI	CCWGG	2 cut(s)	871, 2449
PspN4I	GGNNCC	7 cut(s)	299, 645, 760, 928, 1029, 1162, 1189
PspPI	GGNCC	1 cut(s)	667
PspPPI	RGGWCCY	1 cut(s)	667
PstI	CTGCAG	1 cut(s)	486
PsuI	RGATCY	4 cut(s)	758, 1442, 2198, 2371
PvuII	CAGCTG	1 cut(s)	2443
RsaI	GTAC	4 cut(s)	580, 910, 1128, 1752
RsaNI	GTAC	4 cut(s)	579, 909, 1127, 1751
RseI	CAYNNNNRTG	5 cut(s)	569, 720, 1307, 2510, 2685
SapI	GCTCTTC	2 cut(s)	1451, 2710
SaqAI	TTAA	6 cut(s)	20, 134, 1077, 1199, 1665, 1953
SatI	GCNGC	6 cut(s)	1137, 1254, 1389, 1620, 2337, 2728
Sau96I	GGNCC	1 cut(s)	667
SchI	GAGTC	2 cut(s)	233, 1635
ScrFI	CCNGG	5 cut(s)	302, 303, 356, 873, 2451
SduI	GDGCHC	1 cut(s)	749
SfaNI	GCATC	8 cut(s)	638, 805, 1240, 1649, 1796, 1859, 1871, 2323
SfcI	CTRYAG	2 cut(s)	482, 1065
SinI	GGWCC	1 cut(s)	667
SmaI	CCCGGG	1 cut(s)	303
SmiMI	CAYNNNNRTG	5 cut(s)	569, 720, 1307, 2510, 2685
SmlI	CTYRAG	2 cut(s)	1694, 2716
SmoI	CTYRAG	2 cut(s)	1694, 2716
SpeI	ACTAGT	1 cut(s)	2813
SphI	GCATGC	2 cut(s)	965, 1000
SseBI	AGGCCT	2 cut(s)	379, 2387
SsiI	CCGC	6 cut(s)	106, 389, 1389, 1567, 2309, 2728
SspI	AATATT	1 cut(s)	1841
SspMI	CTAG	5 cut(s)	803, 1509, 1757, 2472, 2814
StuI	AGGCCT	2 cut(s)	379, 2387
StyD4I	CCNGG	5 cut(s)	300, 301, 354, 871, 2449
StyI	CCWWGG	5 cut(s)	153, 564, 802, 988, 1654
TaaI	ACNGT	9 cut(s)	515, 527, 578, 1058, 1204, 1423, 1577, 1690, 2612
TaiI	ACGT	4 cut(s)	335, 440, 880, 1009
TaqI	TCGA	5 cut(s)	130, 543, 774, 2146, 2258
TatI	WGTACW	2 cut(s)	908, 1750
TauI	GCSGC	2 cut(s)	1391, 2730
TfiI	GAWTC	8 cut(s)	158, 206, 458, 617, 635, 1469, 2051, 2156
Tru1I	TTAA	6 cut(s)	20, 134, 1077, 1199, 1665, 1953
Tru9I	TTAA	6 cut(s)	20, 134, 1077, 1199, 1665, 1953
TscAI	CASTG	7 cut(s)	294, 406, 1048, 1882, 1972, 2093, 2282
TseFI	GTSAC	4 cut(s)	225, 333, 1967, 2503
TseI	GCWGC	4 cut(s)	1136, 1253, 1619, 2336
Tsp45I	GTSAC	4 cut(s)	225, 333, 1967, 2503
TspDTI	ATGAA	9 cut(s)	441, 561, 800, 1334, 1383, 1695, 2199, 2474, 2725
TspMI	CCCGGG	1 cut(s)	301
TspRI	CASTG	7 cut(s)	294, 406, 1048, 1882, 1972, 2093, 2282
Van91I	CCANNNNNTGG	1 cut(s)	755
VpaK11BI	GGWCC	1 cut(s)	667
XapI	RAATTY	6 cut(s)	168, 583, 1378, 1427, 1767, 2212
XceI	RCATGY	4 cut(s)	287, 965, 1000, 2352
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1153
XmaI	CCCGGG	1 cut(s)	301
XmaJI	CCTAGG	1 cut(s)	802
XmiI	GTMKAC	2 cut(s)	832, 2036
XmnI	GAANNNNTTC	1 cut(s)	1767
XspI	CTAG	5 cut(s)	803, 1509, 1757, 2472, 2814
Zsp2I	ATGCAT	2 cut(s)	631, 1002

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.