Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0008066

Physical Location & Seq

Genomic Coordinates Reverse (-)

9067670 .. 9074955

Gene Structure 7286 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4269 bp

ATGGAATCGGCTGGTGGTGGTATAAGGGAGACATCTGTTATAGTTGTGACATTGGATACTGGTGAAGTATATATAATTGCGAGTTTGTCTTCTAGGCTCGACACTCAGGTCATACATGTTGATCCCACAACTGGAGTGCTTCGCTACAATGCGAAGCCAGGATTTGATGTTTTCAAATCCGAAAAGGAAGCATTGGATTACATTACAGACGGATCACACTGGTCGTGTAGGAGTACGACCTATGCGCATGCAATATTAGGTTATGCTGCTTTGGGCAGCATTGGTTTGCTTCTCGTGGCCACCAAGTTGACTGCTAGTGTCCCGAATTTGCCTGGTGGAGGGTGCATTTATACGGTGACTGAGAGCCAATGGATCAAGATTCAACTTCAGAATCCACAACCACAAGGGAAAGGAGAAGAAAAGAATGTTAATGAATTAACTGATCTTGACATTAATGGGAAGCACTACTTTTGTGAGGCAAGGGATATCACTCGACCATTTCCTAGCCGTATGTGCTTGCGTGAGCCTGATGATGAATTTGTTTGGAACACCTGGTTCTCGATGCCTTTCAAAAACATTGGGTTGCCGCAGCATTGTGTCACTCTTCTGCAGGGTTTTGCGGAGTGTCGAACTTTTGGAACGTTAGGGAAGGTGGACGGAATTGTTGCTCTCATAGCTCGTCGTAGTAGGTTGCATCCTGGTACTCGGTACTTGGCTAGGGGATTGAATTCTTGTTTTAGCACAGGAAATGAAGTGGAGTGTGAGCAACTTGTATGGGTCCCTAGAAGGGCTGGTCAAACTGTTCCTTTTAACACATACGTGTGGCGACGCGGCACAATTCCAATTTGGCGGGTTGCAGATTTAAAGATTACTCCTGCAGAAGCAGAAATATACGTTTCGGATAGTGATCCTTATAAAGGAAGTTCGGAGTACTACCAGAGATTGAGTAAGAGGTATGATGCACGAAATTTTGATGTAGCCGTTGGGGGGAGTCGGAATAGAAAAGCGTTTGTGCCAATTGTATGCATCAACTTGCTTAGGAGTGAAGAAGGAAAGTCAGAACGTATTTTAGTTCAACATTTTGAGGAATCTTTAAACTACATAAAGTCAACTGGAAAACTTCCTTATACTCGAATTCACTTGATAAATTATGACTGGCATGCCAGTATAAAGTTAAAAGGTGAACAGCAAACCATTGAAGGATTATGGAAACATCTAAAAGCACACTCTGTTTCCGTAGGCATTTCTGAAGGAGATTTTTTGCCTTTGCGGGAAAGAATTAAGGACTGCAGAGGAGAAATTATCTACAATGATGACTTTGAAGGCGCCTTCTGCTTAAGATCGCGTCAAAATGGTGTGATACGTTTCAACTGTGCTGATTCTTTGGATAGGACAAATGCTGCAAGTTATTTTGGCTCCCTCCAGGTTTTCGTGGAGCAGTGTAGGCGGCTTTGTATATCGCTTGATAGTGATTTGGCATTTGGTTATCAGTCAATGAGTAATTATGGTGGCTATACTGCTCCTTTGCCACCAGGCTGGGAGAAGAGATCTGATGCAGTAACAGGGAAAACTTTTTATATTGATCACAATACTAGGACCACAACATGGACGCATCCGTGTCCCGATAAACCTTGGAAGAGATTTGATATGGCATTTGAGGAATTTAAGAGGTCAACAATTTTATCACCGGTATCCCAGCTTGCTGATATTTTTCTGCTTGCGGGTGATATTCATGCAACACTTTATACTGGTTCGAAAGCTATGCATAGCCAAATCCTTAGCATCTTCAATGACGATGCAGGAAAATTCAAACAATTTTCTGCAGCACAGAATATGAAAATTACTTTGCAGCGGAGATATAAAAATGCAGTTGTAGACAGCTCTCGTCAAAAGCAACTGGAAATATTTCTTGGCATGAGACTATTCAAGCATCTTCCATCAGTTTCTTTTCACCCTCTTAACGTCGTCTCTCGACCATCTGGTTTCTTCCTTAAGCCTGTTGCTAACATGTTTCCAAGTTCCAATGGTGGAGCCAGTCTTCTGAGTTTCAAGAGAAAGGATCTAGTCTGGGTTTGCCCACAGGCCGCAGATGTTGTCGAACTTTTTATCTATCTAGGTGAACCTTGCCATGTTTGTCAGCTTCTTCTCACAATATCCCATGGTGTAGATGATTCAACTTATCCATCAACAGTTGATGTAAGGACAGGACGCTTGGAGGGTGCTTCGATACCTCATTGTGTAAATGGAACAAACCTCTTGATACCCTTAACAGGGGCAATTAGTCCAGAGGATATGGCTGTAACAGGAGCTGGTTCACGTCTTCATGCTCAAGATACATCTACCCTTCCATTATTGTATGATTTTGAAGAACTGGAAGGAGAACTTGACTTCCTTACTCGTGTAGTTGCCCTTACATTTTATCCTGCCGTCTCTGGAAAGAGCCCTATCACTTTTGGTGAGATAGAAGTCCTTGGAGTTTCTCTTCCTTGGAAGGGTGTATTTACTAACGAAGGCCCTGGTGCAAGATTACCCGAACAAGCTAAAAACCTTCAGAATGAAAACAATCTTTTTTCCTCTGGGTCAAAAACCAATCCATTTTCTGGTGCTTCATCTAATGAAAATGTGATGGTACCAGTGCAACCAAGTGCATCTGCCAACAATTTGGTTGACCTATTGACTGGAGAGGTTGTGCTTTCAGAACACTTTGCTGCACCAGTGATAGGAAATGCTGAGGACAACGGAGGTGACTTGCTTGATTTCTTGGACCAAGCTATTGTTGAATATCATGGTGCTGAAACTGATCATAAATCACATGATGGAAAACCTTCAGATAGCAGTTCCCAGCAGTACATTGATTGTTTGAAATCCATTGCTGGGCCACGTATGGAAAAAAAACTAAACTTCATGGAAGCCATGAAACTTGAAATTGAACGCCTTCGGCTGGACATTTCTGCTGCTGAAAGGGATAGAGCTTTGTTAACTATAGGAACTGTTCCTGCTACTCTAAACCCTAATGTTTTACTTGATGAACAATACATGGGAAGAATGTGTAGAGTTGCAAACTCCCTTGCACTGCTTGGACAAGCATCCTTGGAAGACAAAATTACATCTGCTGTTGGTCTTGAGACTACTGATGATACTGCAATAGATTTTTGGAATATAACTAGCTTTGGTGAGCGCTGTTATGGCGGCATGTGTGAGGTGCGTGCTGAAACTAATGCACCTACACGTGCTTCCTTTTCGGAATCATCAGGGGGAGTTCCCCCATCTCTCTTCTTATGTTCCCAATGTGAAAGGAAAGTTTGTAAAGTTTGCGGTGCTGGGAGAGGGGCCCTTCTGGTTGCAGGCTATGGCTCAAGGGAGGCCATGAGTTACAATGGTGTGGTAAATCAGGGGGGATCAGGCCGTGGTGTCCAGGTTGATGTCTCAACAAATCATACAGTCGTGCTGGATAGTGTTATTTGTAAAAGATGCTGCGATGATACTGTGCTTGATGCATTGATCTTGGACTACGTCAGGGTCTTGGTAAGCAAGAGGAGAAGTGCCCGTGCAGATTCTGCTGCCCATGAGGCCTTGAACCAAGTGATTGGATATTCGGTAAGGAATTGTCTTTCTGAAAGGAATCAGTCTCCGGATAGACCGAGAACTATTAAAGTTCTGCAGAAATTACTTGGCAGTGTGGAATCGCTAGCTGAGTTTCCATTTGCCAGCTTTTTACACTCGGTTGAAACGGGAGTAGATTCAGCACCATTCTTGTCCTTGCTTGCTCCTCTTGAATCTGGATCACGACATTCATACTGGAAAGCTCCTCCTAGTACCACTAGTGTTGAGTTCGTTATTGTTCTTGGCACCCTTTCTGATGTTAGTGGGGTTGTTTTGCTTATTAGTCCATGTGGTTATTCTGAGGTTGATGCTCCCACCGTGCAAATCTGGGCCAGCAATAAAATACACAAAGAAGAAAGGTCATGCATGGGAAAATGGGATTTGCAGTCCCAGATCACGTCTTCCTCAGAATATTATGGACCAGAAAAGTCGGTTAGAGAAAATGAAGTGCCTAGGCATGTGAAGTTTGAATTTGGGAATCCAGTTCGATGCCGCATTATTTGGATAACATTACGCCTTCAACGACCTGGTTCAAAATCTCTTAATTTGGACAACTTGAATCTCTTGTCTCTTGATGAAAATCCATTTGCAGAAGTAACTCGGCGTGCCTCTTTTGGAGGAAAAGTTGAAAGAGATCACTCATTCATGCCAAAAGGATACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0031348 GO:0033612 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0060548 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Syja_N WW

Protein Analysis

1423

Amino Acids

157.23

Weight (kDa)

6.24

Isoelectric Point (pI)

40.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syja_N	PF02383	83 - 390	4.7e-39	SacI homology domain
WW	PF00397	509 - 539	6.4e-12	WW domain
SAC9_GBDL_1st	PF24790	653 - 830	1.7e-88	SAC9 first GBDL domain
SAC9_C8D	PF24791	1055 - 1166	3.2e-19	SAC9 C8D domain
SAC9_GBDL_2nd	PF24789	1228 - 1414	6.9e-92	SAC9 second GBDL domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008066)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59770 AT3G59770 AT3G59770 AT3G59770
fragaria_vesca	FvH4_1g00530 FvH4_1g00530
malus_domestica	MD02G1004400.v1.1 MD15G1148100.v1.1
prunus_persica	Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1
pyrus_communis	pycom02g00290 pycom15g13240
rosa_chinensis	RchiOBHm_Chr2g0085161
rosa_laevigata	RLG00000015661
rosa_multiflora	Rmu_sc0008647.1_g000009
rosa_roxburghii	Rroxscaffold_2G00155600
rosa_rugosa	Rorug01G0458000
rosa_samantha	Rh2AG006300 Rh2BG007100 Rh2CG007900 Rh2DG008100
rosa_wichuraiana	Rw2G000600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	915
AasI	GACNNNNNNGTC	1 cut(s)	107
Acc16I	TGCGCA	1 cut(s)	246
Acc65I	GGTACC	1 cut(s)	2629
AccB1I	GGYRCC	3 cut(s)	1325, 2629, 3848
AccB7I	CCANNNNNTGG	2 cut(s)	1605, 2600
AccI	GTMKAC	1 cut(s)	1875
AccII	CGCG	2 cut(s)	831, 1345
AccIII	TCCGGA	1 cut(s)	3631
AclI	AACGTT	1 cut(s)	641
AclWI	GGATC	7 cut(s)	116, 220, 380, 902, 2067, 3406, 3790
AcoI	YGGCCR	1 cut(s)	297
AcsI	RAATTY	8 cut(s)	325, 536, 727, 967, 1134, 1661, 1805, 4073
AcuI	CTGAAG	4 cut(s)	371, 1269, 2534, 2811
AcvI	CACGTG	1 cut(s)	3230
AcyI	GRCGYC	1 cut(s)	1326
AfaI	GTAC	7 cut(s)	235, 703, 710, 932, 2631, 2849, 3817
AfeI	AGCGCT	1 cut(s)	3179
AflII	CTTAAG	2 cut(s)	1336, 1991
AflIII	ACRYGT	4 cut(s)	115, 819, 2007, 3227
AgeI	ACCGGT	1 cut(s)	1687
AhlI	ACTAGT	1 cut(s)	3821
AjiI	CACGTC	2 cut(s)	2318, 4002
AjnI	CCWGG	9 cut(s)	157, 331, 551, 697, 1422, 1531, 2515, 3414, 4129
AjuI	GAANNNNNNNTTGG	2 cut(s)	1893, 1925
AleI	CACNNNNGTG	1 cut(s)	818
AloI	GAACNNNNNNTCC	2 cut(s)	2373, 2405
Alw26I	GTCTC	8 cut(s)	23, 1912, 1972, 2434, 3119, 3430, 3633, 4176
AlwI	GGATC	7 cut(s)	116, 220, 380, 902, 2067, 3406, 3790
AlwNI	CAGNNNCTG	2 cut(s)	2309, 3557
Aor13HI	TCCGGA	1 cut(s)	3631
Aor51HI	AGCGCT	1 cut(s)	3179
AoxI	GGCC	9 cut(s)	297, 2082, 2512, 2876, 3330, 3363, 3403, 3570, 3933
ApaI	GGGCCC	1 cut(s)	3334
ApoI	RAATTY	8 cut(s)	325, 536, 727, 967, 1134, 1661, 1805, 4073
AseI	ATTAAT	1 cut(s)	453
AsiGI	ACCGGT	1 cut(s)	1687
Asp700I	GAANNNNTTC	3 cut(s)	1905, 2824, 2934
Asp718I	GGTACC	1 cut(s)	2629
AspA2I	CCTAGG	1 cut(s)	4055
AspLEI	GCGC	3 cut(s)	247, 1328, 3180
AspS9I	GGNCC	9 cut(s)	778, 1596, 2513, 2764, 2876, 3330, 3331, 3933, 4022
AsuII	TTCGAA	1 cut(s)	1754
AsuNHI	GCTAGC	1 cut(s)	3688
AvaII	GGWCC	4 cut(s)	778, 1596, 2764, 4022
AvrII	CCTAGG	1 cut(s)	4055
BaeGI	GKGCMC	2 cut(s)	3334, 3547
BalI	TGGCCA	1 cut(s)	299
BanI	GGYRCC	3 cut(s)	1325, 2629, 3848
BanII	GRGCYC	2 cut(s)	2444, 3334
BauI	CACGAG	2 cut(s)	293, 2397
BbrPI	CACGTG	1 cut(s)	3230
BbsI	GAAGAC	5 cut(s)	81, 2030, 2312, 3102, 3996
BbvCI	CCTCAGC	1 cut(s)	2730
BccI	CCATC	6 cut(s)	1945, 1984, 2191, 2620, 2810, 3274
BceAI	ACGGC	4 cut(s)	492, 965, 2411, 3390
BcgI	CGANNNNNNTGC	2 cut(s)	1744, 1778
BciT130I	CCWGG	9 cut(s)	159, 333, 553, 699, 1424, 1533, 2517, 3416, 4131
BciVI	GTATCC	3 cut(s)	49, 1702, 4253
BclI	TGATCA	2 cut(s)	1582, 2800
BcoDI	GTCTC	8 cut(s)	23, 1912, 1972, 2434, 3119, 3430, 3633, 4176
BcuI	ACTAGT	1 cut(s)	3821
BfmI	CTRYAG	6 cut(s)	608, 876, 1288, 1821, 2982, 3659
BfoI	RGCGCY	2 cut(s)	1329, 3181
BfrI	CTTAAG	2 cut(s)	1336, 1991
BfuI	GTATCC	3 cut(s)	49, 1702, 4253
BglI	GCCNNNNNGGC	1 cut(s)	3569
BglII	AGATCT	1 cut(s)	1547
BlnI	CCTAGG	1 cut(s)	4055
BmcAI	AGTACT	1 cut(s)	932
Bme1390I	CCNGG	9 cut(s)	159, 333, 553, 699, 1424, 1533, 2517, 3416, 4131
Bme18I	GGWCC	4 cut(s)	778, 1596, 2764, 4022
BmgBI	CACGTC	2 cut(s)	2318, 4002
BmgT120I	GGNCC	9 cut(s)	778, 1596, 2513, 2764, 2876, 3330, 3331, 3933, 4022
BmiI	GGNNCC	9 cut(s)	779, 780, 1327, 1417, 2032, 2631, 3331, 3332, 3850
BmrFI	CCNGG	9 cut(s)	159, 333, 553, 699, 1424, 1533, 2517, 3416, 4131
BmtI	GCTAGC	1 cut(s)	3692
BpiI	GAAGAC	5 cut(s)	81, 2030, 2312, 3102, 3996
BpmI	CTGGAG	3 cut(s)	153, 1406, 2700
Bpu10I	CCTNAGC	3 cut(s)	1037, 1778, 2730
Bpu14I	TTCGAA	1 cut(s)	1754
BpuEI	CTTGAG	3 cut(s)	2313, 3143, 3340
BsaAI	YACGTR	3 cut(s)	820, 2882, 3230
BsaBI	GATNNNNATC	2 cut(s)	906, 4182
BsaHI	GRCGYC	1 cut(s)	1326
BsaJI	CCNNGG	8 cut(s)	1631, 2158, 2470, 2486, 2515, 3090, 3406, 4055
BsaWI	WCCGGW	2 cut(s)	1687, 3631
BsaXI	ACNNNNNCTCC	4 cut(s)	2673, 2703, 4212, 4242
Bse118I	RCCGGY	1 cut(s)	1687
Bse3DI	GCAATG	1 cut(s)	2868
Bse8I	GATNNNNATC	2 cut(s)	906, 4182
BseAI	TCCGGA	1 cut(s)	3631
BseBI	CCWGG	9 cut(s)	159, 333, 553, 699, 1424, 1533, 2517, 3416, 4131
BseDI	CCNNGG	8 cut(s)	1631, 2158, 2470, 2486, 2515, 3090, 3406, 4055
BseGI	GGATG	3 cut(s)	694, 1612, 3086
BseJI	GATNNNNATC	2 cut(s)	906, 4182
BseMI	GCAATG	1 cut(s)	2868
BseMII	CTCAG	7 cut(s)	119, 351, 2033, 2721, 3684, 3894, 4023
BseRI	GAGGAG	4 cut(s)	1308, 3550, 3759, 3798
BseSI	GKGCMC	2 cut(s)	3334, 3547
BseYI	CCCAGC	5 cut(s)	1536, 1695, 2841, 2873, 3320
BsgI	GTGCAG	2 cut(s)	2694, 3570
Bsh1236I	CGCG	2 cut(s)	831, 1345
BshFI	GGCC	9 cut(s)	299, 2084, 2514, 2878, 3332, 3365, 3405, 3572, 3935
BshNI	GGYRCC	3 cut(s)	1325, 2629, 3848
BshTI	ACCGGT	1 cut(s)	1687
BsiSI	CCGG	2 cut(s)	1688, 3632
BslFI	GGGAC	4 cut(s)	305, 764, 1605, 3976
BsmAI	GTCTC	8 cut(s)	23, 1912, 1972, 2434, 3119, 3430, 3633, 4176
BsmBI	CGTCTC	2 cut(s)	1972, 2434
BsmFI	GGGAC	4 cut(s)	305, 764, 1605, 3976
BsnI	GGCC	9 cut(s)	299, 2084, 2514, 2878, 3332, 3365, 3405, 3572, 3935
Bsp119I	TTCGAA	1 cut(s)	1754
Bsp120I	GGGCCC	1 cut(s)	3330
Bsp1286I	GDGCHC	3 cut(s)	2444, 3334, 3547
Bsp13I	TCCGGA	1 cut(s)	3631
Bsp19I	CCATGG	1 cut(s)	2158
BspANI	GGCC	9 cut(s)	299, 2084, 2514, 2878, 3332, 3365, 3405, 3572, 3935
BspCNI	CTCAG	7 cut(s)	118, 352, 2034, 2722, 3685, 3895, 4022
BspEI	TCCGGA	1 cut(s)	3631
BspFNI	CGCG	2 cut(s)	831, 1345
BspLI	GGNNCC	9 cut(s)	779, 780, 1327, 1417, 2032, 2631, 3331, 3332, 3850
BspMAI	CTGCAG	5 cut(s)	612, 880, 1292, 1825, 3663
BspOI	GCTAGC	1 cut(s)	3692
BspPI	GGATC	7 cut(s)	116, 220, 380, 902, 2067, 3406, 3790
BspT104I	TTCGAA	1 cut(s)	1754
BspT107I	GGYRCC	3 cut(s)	1325, 2629, 3848
BspTI	CTTAAG	2 cut(s)	1336, 1991
BsrDI	GCAATG	1 cut(s)	2868
BsrFI	RCCGGY	1 cut(s)	1687
BssAI	RCCGGY	1 cut(s)	1687
BssECI	CCNNGG	8 cut(s)	1631, 2158, 2470, 2486, 2515, 3090, 3406, 4055
BssNI	GRCGYC	1 cut(s)	1326
BssSI	CACGAG	2 cut(s)	293, 2397
BssT1I	CCWWGG	6 cut(s)	1631, 2158, 2470, 2486, 3090, 4055
Bst2BI	CACGAG	2 cut(s)	293, 2397
Bst2UI	CCWGG	9 cut(s)	159, 333, 553, 699, 1424, 1533, 2517, 3416, 4131
Bst4CI	ACNGT	8 cut(s)	355, 802, 1373, 2191, 2992, 3442, 3487, 3922
Bst6I	CTCTTC	5 cut(s)	609, 1538, 1631, 2487, 3278
BstACI	GRCGYC	1 cut(s)	1326
BstAFI	CTTAAG	2 cut(s)	1336, 1991
BstAPI	GCANNNNNTGC	1 cut(s)	3557
BstBAI	YACGTR	3 cut(s)	820, 2882, 3230
BstBI	TTCGAA	1 cut(s)	1754
BstDEI	CTNAG	9 cut(s)	105, 360, 1037, 1778, 2042, 2730, 3693, 3903, 4009
BstDSI	CCRYGG	2 cut(s)	2158, 3406
BstENI	CCTNNNNNAGG	2 cut(s)	336, 915
BstF5I	GGATG	3 cut(s)	694, 1612, 3086
BstFNI	CGCG	2 cut(s)	831, 1345
BstH2I	RGCGCY	2 cut(s)	1329, 3181
BstHHI	GCGC	3 cut(s)	247, 1328, 3180
BstMAI	GTCTC	8 cut(s)	23, 1912, 1972, 2434, 3119, 3430, 3633, 4176
BstMWI	GCNNNNNNNGC	7 cut(s)	513, 674, 1332, 1444, 3186, 3557, 3569
BstNI	CCWGG	9 cut(s)	159, 333, 553, 699, 1424, 1533, 2517, 3416, 4131
BstNSI	RCATGY	6 cut(s)	119, 251, 1163, 2011, 3196, 4064
BstSCI	CCNGG	9 cut(s)	157, 331, 551, 697, 1422, 1531, 2515, 3414, 4129
BstSFI	CTRYAG	6 cut(s)	608, 876, 1288, 1821, 2982, 3659
BstSLI	GKGCMC	2 cut(s)	3334, 3547
BstUI	CGCG	2 cut(s)	831, 1345
BstV2I	GAAGAC	5 cut(s)	81, 2030, 2312, 3102, 3996
BstX2I	RGATCY	2 cut(s)	1547, 2059
BstXI	CCANNNNNNTGG	3 cut(s)	1536, 2662, 3587
BstYI	RGATCY	2 cut(s)	1547, 2059
BsuI	GTATCC	3 cut(s)	49, 1702, 4253
BsuRI	GGCC	9 cut(s)	299, 2084, 2514, 2878, 3332, 3365, 3405, 3572, 3935
BtgI	CCRYGG	2 cut(s)	2158, 3406
BtgZI	GCGATG	1 cut(s)	3492
BtrI	CACGTC	2 cut(s)	2318, 4002
BtsCI	GGATG	3 cut(s)	694, 1612, 3086
BtsI	GCAGTG	3 cut(s)	1445, 3071, 3682
BtsIMutI	CAGTG	6 cut(s)	217, 1445, 2640, 2721, 3071, 3682
CaiI	CAGNNNCTG	2 cut(s)	2309, 3557
CfoI	GCGC	3 cut(s)	247, 1328, 3180
Cfr10I	RCCGGY	1 cut(s)	1687
Cfr13I	GGNCC	9 cut(s)	778, 1596, 2513, 2764, 2876, 3330, 3331, 3933, 4022
CseI	GACGC	4 cut(s)	837, 1334, 1618, 2217
CsiI	ACCWGGT	2 cut(s)	551, 4129
Csp6I	GTAC	7 cut(s)	234, 702, 709, 931, 2630, 2848, 3816
CspAI	ACCGGT	1 cut(s)	1687
CspCI	CAANNNNNGTGG	2 cut(s)	3808, 3843
CviQI	GTAC	7 cut(s)	234, 702, 709, 931, 2630, 2848, 3816
DdeI	CTNAG	9 cut(s)	105, 360, 1037, 1778, 2042, 2730, 3693, 3903, 4009
DinI	GGCGCC	1 cut(s)	1327
DraI	TTTAAA	2 cut(s)	864, 1095
DrdI	GACNNNNNNGTC	1 cut(s)	107
DseDI	GACNNNNNNGTC	1 cut(s)	107
EaeI	YGGCCR	1 cut(s)	297
Eam1104I	CTCTTC	5 cut(s)	609, 1538, 1631, 2487, 3278
EarI	CTCTTC	5 cut(s)	609, 1538, 1631, 2487, 3278
Eco130I	CCWWGG	6 cut(s)	1631, 2158, 2470, 2486, 3090, 4055
Eco147I	AGGCCT	1 cut(s)	3572
Eco24I	GRGCYC	2 cut(s)	2444, 3334
Eco32I	GATATC	1 cut(s)	487
Eco47I	GGWCC	4 cut(s)	778, 1596, 2764, 4022
Eco47III	AGCGCT	1 cut(s)	3179
Eco57I	CTGAAG	4 cut(s)	371, 1269, 2534, 2811
Eco72I	CACGTG	1 cut(s)	3230
EcoNI	CCTNNNNNAGG	2 cut(s)	336, 915
EcoO109I	RGGNCCY	4 cut(s)	778, 2513, 3330, 3331
EcoRI	GAATTC	2 cut(s)	727, 1134
EcoRII	CCWGG	9 cut(s)	157, 331, 551, 697, 1422, 1531, 2515, 3414, 4129
EcoRV	GATATC	1 cut(s)	487
EcoT14I	CCWWGG	6 cut(s)	1631, 2158, 2470, 2486, 3090, 4055
EcoT22I	ATGCAT	4 cut(s)	1028, 1767, 3499, 3971
EcoT38I	GRGCYC	2 cut(s)	2444, 3334
EgeI	GGCGCC	1 cut(s)	1327
EheI	GGCGCC	1 cut(s)	1327
ErhI	CCWWGG	6 cut(s)	1631, 2158, 2470, 2486, 3090, 4055
Esp3I	CGTCTC	2 cut(s)	1972, 2434
FalI	AAGNNNNNCTT	4 cut(s)	452, 484, 2367, 2399
FaqI	GGGAC	4 cut(s)	305, 764, 1605, 3976
FauI	CCCGC	3 cut(s)	843, 1263, 1714
FbaI	TGATCA	2 cut(s)	1582, 2800
FblI	GTMKAC	1 cut(s)	1875
FokI	GGATG	3 cut(s)	681, 1599, 3073
FriOI	GRGCYC	2 cut(s)	2444, 3334
FspAI	RTGCGCAY	1 cut(s)	246
FspI	TGCGCA	1 cut(s)	246
GlaI	GCGC	3 cut(s)	246, 1327, 3179
GsaI	CCCAGC	5 cut(s)	1540, 1699, 2845, 2877, 3324
GsuI	CTGGAG	3 cut(s)	153, 1406, 2700
HaeII	RGCGCY	2 cut(s)	1329, 3181
HaeIII	GGCC	9 cut(s)	299, 2084, 2514, 2878, 3332, 3365, 3405, 3572, 3935
HapII	CCGG	2 cut(s)	1688, 3632
HgaI	GACGC	4 cut(s)	837, 1334, 1618, 2217
HhaI	GCGC	3 cut(s)	247, 1328, 3180
Hin1I	GRCGYC	1 cut(s)	1326
Hin6I	GCGC	3 cut(s)	245, 1326, 3178
HinP1I	GCGC	3 cut(s)	245, 1326, 3178
HincII	GTYRAC	5 cut(s)	309, 1110, 1674, 2668, 2979
HindII	GTYRAC	5 cut(s)	309, 1110, 1674, 2668, 2979
HpaI	GTTAAC	1 cut(s)	2979
HpaII	CCGG	2 cut(s)	1688, 3632
Hpy99I	CGWCG	3 cut(s)	684, 831, 1967
HpyCH4III	ACNGT	8 cut(s)	355, 802, 1373, 2191, 2992, 3442, 3487, 3922
HpyF10VI	GCNNNNNNNGC	7 cut(s)	513, 674, 1332, 1444, 3186, 3557, 3569
HpyF3I	CTNAG	9 cut(s)	105, 360, 1037, 1778, 2042, 2730, 3693, 3903, 4009
Hsp92I	GRCGYC	1 cut(s)	1326
HspAI	GCGC	3 cut(s)	245, 1326, 3178
KasI	GGCGCC	1 cut(s)	1325
KflI	GGGWCCC	1 cut(s)	778
Kpn2I	TCCGGA	1 cut(s)	3631
KpnI	GGTACC	1 cut(s)	2633
Ksp22I	TGATCA	2 cut(s)	1582, 2800
KspAI	GTTAAC	1 cut(s)	2979
LmnI	GCTCC	8 cut(s)	1421, 1435, 1525, 2030, 2306, 3772, 3811, 3919
MabI	ACCWGGT	2 cut(s)	551, 4129
MaeIII	GTNAC	8 cut(s)	46, 355, 598, 1558, 2299, 2744, 3371, 4198
MfeI	CAATTG	1 cut(s)	1017
MflI	RGATCY	2 cut(s)	1547, 2059
MhlI	GDGCHC	3 cut(s)	2444, 3334, 3547
MlsI	TGGCCA	1 cut(s)	299
MluNI	TGGCCA	1 cut(s)	299
Mly113I	GGCGCC	1 cut(s)	1326
MlyI	GAGTC	1 cut(s)	1000
MmeI	TCCRAC	1 cut(s)	974
Mox20I	TGGCCA	1 cut(s)	299
Mph1103I	ATGCAT	4 cut(s)	1028, 1767, 3499, 3971
MroI	TCCGGA	1 cut(s)	3631
MroXI	GAANNNNTTC	3 cut(s)	1905, 2824, 2934
MscI	TGGCCA	1 cut(s)	299
MslI	CAYNNNNRTG	2 cut(s)	818, 820
Msp20I	TGGCCA	1 cut(s)	299
MspA1I	CMGCKG	1 cut(s)	1852
MspCI	CTTAAG	2 cut(s)	1336, 1991
MspI	CCGG	2 cut(s)	1688, 3632
MspR9I	CCNGG	9 cut(s)	159, 333, 553, 699, 1424, 1533, 2517, 3416, 4131
MunI	CAATTG	1 cut(s)	1017
MvaI	CCWGG	9 cut(s)	159, 333, 553, 699, 1424, 1533, 2517, 3416, 4131
MvnI	CGCG	2 cut(s)	831, 1345
MwoI	GCNNNNNNNGC	7 cut(s)	513, 674, 1332, 1444, 3186, 3557, 3569
NarI	GGCGCC	1 cut(s)	1326
NcoI	CCATGG	1 cut(s)	2158
NheI	GCTAGC	1 cut(s)	3688
NlaIV	GGNNCC	9 cut(s)	779, 780, 1327, 1417, 2032, 2631, 3331, 3332, 3850
NmeAIII	GCCGAG	1 cut(s)	4183
NmuCI	GTSAC	4 cut(s)	46, 355, 598, 2744
NsbI	TGCGCA	1 cut(s)	246
NsiI	ATGCAT	4 cut(s)	1028, 1767, 3499, 3971
NspI	RCATGY	6 cut(s)	119, 251, 1163, 2011, 3196, 4064
NspV	TTCGAA	1 cut(s)	1754
OliI	CACNNNNGTG	1 cut(s)	818
PaeI	GCATGC	2 cut(s)	251, 1163
PceI	AGGCCT	1 cut(s)	3572
PciI	ACATGT	2 cut(s)	115, 2007
PcsI	WCGNNNNNNNCGW	1 cut(s)	4123
PdmI	GAANNNNTTC	3 cut(s)	1905, 2824, 2934
PflFI	GACNNNGTC	1 cut(s)	3512
PflMI	CCANNNNNTGG	2 cut(s)	1605, 2600
PinAI	ACCGGT	1 cut(s)	1687
PleI	GAGTC	1 cut(s)	999
PluTI	GGCGCC	1 cut(s)	1329
PmaCI	CACGTG	1 cut(s)	3230
PmlI	CACGTG	1 cut(s)	3230
PpsI	GAGTC	1 cut(s)	999
Ppu21I	YACGTR	3 cut(s)	820, 2882, 3230
PpuMI	RGGWCCY	1 cut(s)	778
PscI	ACATGT	2 cut(s)	115, 2007
PshBI	ATTAAT	1 cut(s)	453
PsiI	TTATAA	1 cut(s)	915
Psp1406I	AACGTT	1 cut(s)	641
Psp5II	RGGWCCY	1 cut(s)	778
Psp6I	CCWGG	9 cut(s)	157, 331, 551, 697, 1422, 1531, 2515, 3414, 4129
PspCI	CACGTG	1 cut(s)	3230
PspFI	CCCAGC	5 cut(s)	1536, 1695, 2841, 2873, 3320
PspGI	CCWGG	9 cut(s)	157, 331, 551, 697, 1422, 1531, 2515, 3414, 4129
PspN4I	GGNNCC	9 cut(s)	779, 780, 1327, 1417, 2032, 2631, 3331, 3332, 3850
PspOMI	GGGCCC	1 cut(s)	3330
PspPI	GGNCC	9 cut(s)	778, 1596, 2513, 2764, 2876, 3330, 3331, 3933, 4022
PspPPI	RGGWCCY	1 cut(s)	778
PstI	CTGCAG	5 cut(s)	612, 880, 1292, 1825, 3663
PstNI	CAGNNNCTG	2 cut(s)	2309, 3557
PsuI	RGATCY	2 cut(s)	1547, 2059
PsyI	GACNNNGTC	1 cut(s)	3512
RsaI	GTAC	7 cut(s)	235, 703, 710, 932, 2631, 2849, 3817
RsaNI	GTAC	7 cut(s)	234, 702, 709, 931, 2630, 2848, 3816
RseI	CAYNNNNRTG	2 cut(s)	818, 820
Sau96I	GGNCC	9 cut(s)	778, 1596, 2513, 2764, 2876, 3330, 3331, 3933, 4022
ScaI	AGTACT	1 cut(s)	932
SchI	GAGTC	1 cut(s)	1000
ScrFI	CCNGG	9 cut(s)	159, 333, 553, 699, 1424, 1533, 2517, 3416, 4131
SduI	GDGCHC	3 cut(s)	2444, 3334, 3547
SexAI	ACCWGGT	2 cut(s)	551, 4129
SfcI	CTRYAG	6 cut(s)	608, 876, 1288, 1821, 2982, 3659
SfoI	GGCGCC	1 cut(s)	1327
SfuI	TTCGAA	1 cut(s)	1754
SinI	GGWCC	4 cut(s)	778, 1596, 2764, 4022
SmiMI	CAYNNNNRTG	2 cut(s)	818, 820
SmlI	CTYRAG	5 cut(s)	1336, 1991, 2328, 3122, 3355
SmoI	CTYRAG	5 cut(s)	1336, 1991, 2328, 3122, 3355
SpeI	ACTAGT	1 cut(s)	3821
SphI	GCATGC	2 cut(s)	251, 1163
SseBI	AGGCCT	1 cut(s)	3572
SspDI	GGCGCC	1 cut(s)	1325
SspI	AATATT	3 cut(s)	255, 1905, 4016
StuI	AGGCCT	1 cut(s)	3572
StyD4I	CCNGG	9 cut(s)	157, 331, 551, 697, 1422, 1531, 2515, 3414, 4129
StyI	CCWWGG	6 cut(s)	1631, 2158, 2470, 2486, 3090, 4055
TaaI	ACNGT	8 cut(s)	355, 802, 1373, 2191, 2992, 3442, 3487, 3922
TaqII	GACCGA	1 cut(s)	3655
TatI	WGTACW	2 cut(s)	930, 2847
TauI	GCSGC	6 cut(s)	589, 834, 1450, 2087, 3192, 4098
TscAI	CASTG	6 cut(s)	224, 1445, 2640, 2721, 3078, 3682
TseFI	GTSAC	4 cut(s)	46, 355, 598, 2744
Tsp45I	GTSAC	4 cut(s)	46, 355, 598, 2744
TspGWI	ACGGA	5 cut(s)	225, 672, 1225, 1605, 2754
TspRI	CASTG	6 cut(s)	224, 1445, 2640, 2721, 3078, 3682
Tth111I	GACNNNGTC	1 cut(s)	3512
Van91I	CCANNNNNTGG	2 cut(s)	1605, 2600
Vha464I	CTTAAG	2 cut(s)	1336, 1991
VpaK11BI	GGWCC	4 cut(s)	778, 1596, 2764, 4022
VspI	ATTAAT	1 cut(s)	453
XagI	CCTNNNNNAGG	2 cut(s)	336, 915
XapI	RAATTY	8 cut(s)	325, 536, 727, 967, 1134, 1661, 1805, 4073
XceI	RCATGY	6 cut(s)	119, 251, 1163, 2011, 3196, 4064
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4260
XmaJI	CCTAGG	1 cut(s)	4055
XmiI	GTMKAC	1 cut(s)	1875
XmnI	GAANNNNTTC	3 cut(s)	1905, 2824, 2934
ZrmI	AGTACT	1 cut(s)	932
Zsp2I	ATGCAT	4 cut(s)	1028, 1767, 3499, 3971

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.