pycom15g13880

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012927
Physical Location & Seq
Reverse (-)
9468688 .. 9469915
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 483 bp
ATGAATAAGGGTGGAGGTGCAGCGTCAGGGGGAGGAGGATTGGGAGGCGCTGGGAGCGGGCCCACCGCGGCTGCTGCTGTGGCGGCAGCTCAGAAGCAGAAGGCGCTGCTTCAGAGAGTGGAGACCGACATCTCCCAAATCGTCGACAATTTCAGCCACCTCGTCCACGTCTCCAGGGTGAATGATCCGCCTGTTAGAAACTCTCAGGAAGCTTTCATGATGGAGATGCGTGCTGCTCGAATGGTCCAGGCAGCTGATTCTCTGCTTAAGTTGGTGTCGGAATTGAAACAGACGGCAATATTTTCAGGGTTTGCCTCCCTCAATGACCATGTAGAGAAAAGAATTGTGGAGTTCAACCAGCAAGCCGAGAACACAGACCGCTTGTTGGCTAGAATCGGAGAGGAGGCAGCTGCTAGCCTCAAGGAGCTCGAATCCCATTACTACTCTTCCGCGCTGAGGACTGCTCAAAACTTGCAGCCATGA

Protein Analysis

161

Amino Acids

17.01

Weight (kDa)

6.42

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med22 PF06179 38 - 137 2.4e-30 Surfeit locus protein 5 subunit 22 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07950 AT1G07950 AT1G16430
fragaria_vesca FvH4_3g13800
malus_domestica MD02G1009700.v1.1
prunus_persica Prupe.7G260300_v2.0.a1 Prupe.7G260300_v2.0.a1
pyrus_communis pycom15g13880
rosa_chinensis RchiOBHm_Chr5g0022991
rosa_laevigata RLG00000032687
rosa_multiflora Rmu_sc0002675.1_g000006
rosa_roxburghii Rroxscaffold_1G00056350
rosa_rugosa Rorug05G0076300
rosa_samantha Rh5AG162500 Rh5BG161700 Rh5CG176400 Rh5DG163100
rosa_wichuraiana Rw5G014640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 57
AccI GTMKAC 1 cut(s) 144
AccII CGCG 2 cut(s) 68, 452
AciI CCGC 7 cut(s) 57, 66, 68, 83, 188, 379, 450
AclWI GGATC 1 cut(s) 179
AcuI CTGAAG 1 cut(s) 95
AfiI CCNNNNNNNGG 2 cut(s) 385, 456
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 2 cut(s) 286, 355
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 2 cut(s) 173, 246
AluBI AGCT 5 cut(s) 89, 212, 254, 410, 427
AluI AGCT 5 cut(s) 89, 212, 254, 410, 427
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 116, 175
AlwI GGATC 1 cut(s) 179
AoxI GGCC 1 cut(s) 59
ApaI GGGCCC 1 cut(s) 63
AspLEI GCGC 3 cut(s) 50, 106, 454
AspS9I GGNCC 3 cut(s) 59, 60, 244
AsuHPI GGTGA 1 cut(s) 190
AsuNHI GCTAGC 1 cut(s) 413
AvaII GGWCC 1 cut(s) 244
BaeGI GKGCMC 1 cut(s) 63
BanII GRGCYC 2 cut(s) 63, 429
Bbv12I GWGCWC 1 cut(s) 429
BbvCI CCTCAGC 1 cut(s) 455
BbvI GCAGC 9 cut(s) 32, 58, 61, 93, 98, 220, 263, 397, 419
BccI CCATC 1 cut(s) 214
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 2 cut(s) 175, 248
BcoDI GTCTC 2 cut(s) 116, 175
BfaI CTAG 2 cut(s) 390, 414
BfoI RGCGCY 2 cut(s) 51, 107
BfrI CTTAAG 1 cut(s) 266
Bme1390I CCNGG 2 cut(s) 175, 248
Bme18I GGWCC 1 cut(s) 244
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 59, 60, 244
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 2 cut(s) 175, 248
BmsI GCATC 1 cut(s) 216
BmtI GCTAGC 1 cut(s) 417
BplI GAGNNNNNCTC 2 cut(s) 448, 480
BpmI CTGGAG 1 cut(s) 157
Bpu10I CCTNAGC 1 cut(s) 455
BpuEI CTTGAG 1 cut(s) 404
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 66, 174
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 456
BseBI CCWGG 2 cut(s) 175, 248
BseDI CCNNGG 2 cut(s) 66, 174
BseLI CCNNNNNNNGG 2 cut(s) 385, 456
BseMII CTCAG 3 cut(s) 104, 218, 446
BseRI GAGGAG 2 cut(s) 48, 416
BseSI GKGCMC 1 cut(s) 63
BseXI GCAGC 9 cut(s) 32, 58, 61, 93, 98, 220, 263, 397, 419
BseYI CCCAGC 1 cut(s) 50
BsgI GTGCAG 1 cut(s) 39
Bsh1236I CGCG 2 cut(s) 68, 452
BshFI GGCC 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 429
BslI CCNNNNNNNGG 2 cut(s) 385, 456
BsmAI GTCTC 2 cut(s) 116, 175
BsmBI CGTCTC 1 cut(s) 175
BsnI GGCC 1 cut(s) 61
Bso31I GGTCTC 1 cut(s) 116
Bsp120I GGGCCC 1 cut(s) 59
Bsp1286I GDGCHC 2 cut(s) 63, 429
Bsp143I GATC 1 cut(s) 184
BspACI CCGC 7 cut(s) 57, 66, 68, 83, 188, 379, 450
BspANI GGCC 1 cut(s) 61
BspCNI CTCAG 3 cut(s) 103, 217, 447
BspFNI CGCG 2 cut(s) 68, 452
BspHI TCATGA 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 61
BspOI GCTAGC 1 cut(s) 417
BspPI GGATC 1 cut(s) 179
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 116
BsrBI CCGCTC 1 cut(s) 57
BssECI CCNNGG 2 cut(s) 66, 174
BssMI GATC 1 cut(s) 184
Bst2UI CCWGG 2 cut(s) 175, 248
Bst6I CTCTTC 1 cut(s) 451
BstAFI CTTAAG 1 cut(s) 266
BstC8I GCNNGC 4 cut(s) 59, 231, 363, 415
BstDEI CTNAG 3 cut(s) 90, 204, 455
BstDSI CCRYGG 1 cut(s) 66
BstFNI CGCG 2 cut(s) 68, 452
BstH2I RGCGCY 2 cut(s) 51, 107
BstHHI GCGC 3 cut(s) 50, 106, 454
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 2 cut(s) 116, 175
BstMBI GATC 1 cut(s) 184
BstMWI GCNNNNNNNGC 5 cut(s) 54, 74, 80, 83, 103
BstNI CCWGG 2 cut(s) 175, 248
BstSCI CCNGG 2 cut(s) 173, 246
BstSLI GKGCMC 1 cut(s) 63
BstUI CGCG 2 cut(s) 68, 452
BstV1I GCAGC 9 cut(s) 32, 58, 61, 93, 98, 220, 263, 397, 419
BsuRI GGCC 1 cut(s) 61
BtgI CCRYGG 1 cut(s) 66
BtrI CACGTC 1 cut(s) 169
Cac8I GCNNGC 4 cut(s) 59, 231, 363, 415
CciI TCATGA 1 cut(s) 216
CfoI GCGC 3 cut(s) 50, 106, 454
Cfr13I GGNCC 3 cut(s) 59, 60, 244
Cfr42I CCGCGG 1 cut(s) 69
CseI GACGC 1 cut(s) 12
CviAII CATG 3 cut(s) 217, 329, 480
DdeI CTNAG 3 cut(s) 90, 204, 455
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
EciI GGCGGA 1 cut(s) 177
Ecl136II GAGCTC 1 cut(s) 427
Eco24I GRGCYC 2 cut(s) 63, 429
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 1 cut(s) 244
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 427
EcoRII CCWGG 2 cut(s) 173, 246
EcoT38I GRGCYC 2 cut(s) 63, 429
Esp3I CGTCTC 1 cut(s) 175
FaeI CATG 3 cut(s) 220, 332, 483
FaiI YATR 3 cut(s) 218, 330, 481
FatI CATG 3 cut(s) 216, 328, 479
FauI CCCGC 1 cut(s) 50
FblI GTMKAC 1 cut(s) 144
FriOI GRGCYC 2 cut(s) 63, 429
FspBI CTAG 2 cut(s) 390, 414
GlaI GCGC 3 cut(s) 49, 105, 453
GsaI CCCAGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 157
HaeII RGCGCY 2 cut(s) 51, 107
HaeIII GGCC 1 cut(s) 61
HgaI GACGC 1 cut(s) 12
HhaI GCGC 3 cut(s) 50, 106, 454
Hin1II CATG 3 cut(s) 220, 332, 483
Hin6I GCGC 3 cut(s) 48, 104, 452
HinP1I GCGC 3 cut(s) 48, 104, 452
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 3 cut(s) 257, 393, 431
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 2 cut(s) 145, 166
Hpy188I TCNGA 4 cut(s) 93, 114, 280, 398
Hpy188III TCNNGA 2 cut(s) 206, 217
Hpy8I GTNNAC 2 cut(s) 145, 166
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 94
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 2 cut(s) 20, 475
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 74, 80, 83, 103
HpyF3I CTNAG 3 cut(s) 90, 204, 455
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 3 cut(s) 220, 332, 483
HspAI GCGC 3 cut(s) 48, 104, 452
KspI CCGCGG 1 cut(s) 69
Kzo9I GATC 1 cut(s) 184
LmnI GCTCC 2 cut(s) 54, 424
Lsp1109I GCAGC 9 cut(s) 32, 58, 61, 93, 98, 220, 263, 397, 419
LweI GCATC 1 cut(s) 216
MaeI CTAG 2 cut(s) 390, 414
MaeII ACGT 1 cut(s) 168
MalI GATC 1 cut(s) 186
MbiI CCGCTC 1 cut(s) 57
MboI GATC 1 cut(s) 184
MboII GAAGA 1 cut(s) 438
MhlI GDGCHC 2 cut(s) 63, 429
MluCI AATT 3 cut(s) 148, 281, 342
MmeI TCCRAC 1 cut(s) 258
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 3 cut(s) 68, 254, 410
MspCI CTTAAG 1 cut(s) 266
MspR9I CCNGG 2 cut(s) 175, 248
MvaI CCWGG 2 cut(s) 175, 248
MvnI CGCG 2 cut(s) 68, 452
MwoI GCNNNNNNNGC 5 cut(s) 54, 74, 80, 83, 103
NdeII GATC 1 cut(s) 184
NheI GCTAGC 1 cut(s) 413
NlaIII CATG 3 cut(s) 220, 332, 483
NlaIV GGNNCC 1 cut(s) 61
NmeAIII GCCGAG 1 cut(s) 391
PagI TCATGA 1 cut(s) 216
PfeI GAWTC 3 cut(s) 257, 393, 431
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 173, 246
PspFI CCCAGC 1 cut(s) 50
PspGI CCWGG 2 cut(s) 173, 246
PspN4I GGNNCC 1 cut(s) 61
PspOMI GGGCCC 1 cut(s) 59
PspPI GGNCC 3 cut(s) 59, 60, 244
PvuII CAGCTG 2 cut(s) 254, 410
SacI GAGCTC 1 cut(s) 429
SacII CCGCGG 1 cut(s) 69
SalI GTCGAC 1 cut(s) 143
SaqAI TTAA 1 cut(s) 267
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 3 cut(s) 59, 60, 244
ScrFI CCNGG 2 cut(s) 175, 248
SduI GDGCHC 2 cut(s) 63, 429
SetI ASST 8 cut(s) 19, 91, 162, 171, 214, 256, 412, 429
SfaNI GCATC 1 cut(s) 216
Sfr303I CCGCGG 1 cut(s) 69
SgrBI CCGCGG 1 cut(s) 69
SinI GGWCC 1 cut(s) 244
SmlI CTYRAG 2 cut(s) 266, 419
SmoI CTYRAG 2 cut(s) 266, 419
Sse9I AATT 3 cut(s) 148, 281, 342
SsiI CCGC 7 cut(s) 57, 66, 68, 83, 188, 379, 450
SspI AATATT 1 cut(s) 300
SspMI CTAG 2 cut(s) 390, 414
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 173, 246
TaiI ACGT 1 cut(s) 171
TaqI TCGA 3 cut(s) 144, 238, 429
TaqII GACCGA 1 cut(s) 140
TasI AATT 3 cut(s) 148, 281, 342
TauI GCSGC 2 cut(s) 71, 86
TfiI GAWTC 3 cut(s) 257, 393, 431
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TspDTI ATGAA 2 cut(s) 17, 205
Vha464I CTTAAG 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 144
XspI CTAG 2 cut(s) 390, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.