pycom15g15290

Equilibrative nucleotide transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013473
Physical Location & Seq
Forward (+)
10640808 .. 10642172
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGGAAGGTGGAGTGAAGGTTAGTGTTGAATATAAAGCTGAGCCAAGAGATGCGTACAAGATTGCTTACATTATTCATTTCTTGCTGGGAGCAGGCAGTTTGCTACCTTGGAATGCATTCATAACCGCCGTTGATTACTTTGGACGTCTCTATCCGACCAAACATGTTGAGAAGGTCTTCTCTGTGGCCTATATGAGCTCTTCGTTGCTCGTATTGCTCGTGATGATGAGCTGGAGTAACTGGTGGGAGAAGATGAGTCCTCGGCTGAGACTCAACTTGGGATTTTCTCTGTTTATTCTATCGTTAATGGTAGCCCCAGTTACGGGCTGGCTTTCGCGCGGTTCTGCGCTGAGTAGCGGGAGAGCTAATGCGGATTATGGTGTGACAGTTGCATCAGTTGTAGTTTGTGGCATAGCCGATGGCTTGGTAGGAGGAAGCTTGATGGGATCGACCGTCAAGCTTCCGAAAAAATACATGCAAGCTGTTTTTGCAGGAACTGCTTCTTCAGGTGTGATAATTTCTCTGCTGAGGATTTCAACCAAAGCAATGCTTCCACAAACCCCGAAAGGACTACAAACAAGCGCGAATTCGTACTTCATCGTCAGCACCGTATTCCTCCTGTGCTGCATCCTTGCCTGCAACTTGTTATACAGGCTGACAGTCGTACAGGAACACTGCAGGCTTGCTCAAGACGAACCCTTTTGCGCAAGACCAAAATTTTGGGACGTAGCACGTAAAGTCCCCATGCCAGCTTTTGGGATTTTCGTAACGTATACAGTGACTCTGTCGATTTTCCCTGGATTTATCGCCGAAAGTCTTGAATCCAAGCTTCTTCAAGATTGGTATCCTATTTTGCTGATCACAGTGTACAATGTTGCAGATTTAGTGGGAAAATCGTTGACTGCAGTTTATCTTCTAAAAAGTACTAAAAGGGCAACATTGGCTTGCATTACAAGGCTCCTATTCTATCCAATCTTCACTGCTTGCCTCCATGGACCTCCATGGCTGAAAGCTGAAACTCCAATGGTGGCTCTCACATTTATGCTTGGACTGACAAATGGTTATCTGACAAGTGTTCTCATGATGATAGTTCCGAAAACTGTGCCGGTTTCAGAAGCAGAGCTGTCTGCAATTGTAATGGTGGTGTTCCTTGGAGTTGGCTTGGTTAGCGGTTCGGTTCTCGGGTGGTTCTGGATTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleoside_tran

Protein Analysis

401

Amino Acids

43.83

Weight (kDa)

9.29

Isoelectric Point (pI)

34.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoside_tran PF01733 131 - 228 4e-19 Nucleoside transporter
Nucleoside_tran PF01733 239 - 398 3.6e-38 Nucleoside transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013473)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02630 AT1G02630
fragaria_vesca FvH4_1g03060
malus_domestica MD02G1029900.v1.1 MD15G1171300.v1.1
prunus_persica Prupe.7G245000_v2.0.a1
pyrus_communis pycom02g02490 pycom15g15290
rosa_chinensis RchiOBHm_Chr2g0088191
rosa_laevigata RLG00000015930
rosa_multiflora Rmu_sc0003275.1_g000069
rosa_roxburghii Rroxscaffold_2G00152840
rosa_rugosa Rorug01G0481700
rosa_samantha Rh2AG036700 Rh2BG035900 Rh2CG037000 Rh2DG036400
rosa_wichuraiana Rw2G002890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 148
Acc16I TGCGCA 1 cut(s) 706
AccB7I CCANNNNNTGG 1 cut(s) 755
AccI GTMKAC 1 cut(s) 773
AccII CGCG 3 cut(s) 337, 339, 584
AciI CCGC 5 cut(s) 126, 339, 357, 371, 1170
AclWI GGATC 1 cut(s) 454
AcsI RAATTY 2 cut(s) 586, 716
AcuI CTGAAG 1 cut(s) 489
AcyI GRCGYC 1 cut(s) 145
AfaI GTAC 5 cut(s) 56, 593, 666, 869, 925
AfiI CCNNNNNNNGG 3 cut(s) 322, 323, 755
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 4 cut(s) 29, 537, 821, 836
AjnI CCWGG 1 cut(s) 796
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 2 cut(s) 152, 262
AlwI GGATC 1 cut(s) 454
AlwNI CAGNNNCTG 1 cut(s) 497
Ama87I CYCGRG 1 cut(s) 1181
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 624
ApoI RAATTY 2 cut(s) 586, 716
ArsI GACNNNNNNTTYG 2 cut(s) 702, 734
Asp700I GAANNNNTTC 3 cut(s) 116, 176, 499
AspLEI GCGC 4 cut(s) 339, 349, 584, 707
AspS9I GGNCC 1 cut(s) 995
AvaI CYCGRG 1 cut(s) 1181
AvaII GGWCC 1 cut(s) 995
BanII GRGCYC 1 cut(s) 200
BauI CACGAG 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 200
BbvCI CCTCAGC 1 cut(s) 527
BbvI GCAGC 1 cut(s) 611
BccI CCATC 2 cut(s) 413, 436
BceAI ACGGC 1 cut(s) 113
BcgI CGANNNNNNTGC 2 cut(s) 1084, 1118
BciT130I CCWGG 1 cut(s) 798
BciVI GTATCC 1 cut(s) 855
BclI TGATCA 1 cut(s) 858
BcoDI GTCTC 2 cut(s) 152, 262
BfmI CTRYAG 2 cut(s) 676, 903
BfuI GTATCC 1 cut(s) 855
BisI GCNGC 1 cut(s) 625
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 626
BmcAI AGTACT 1 cut(s) 925
Bme1390I CCNGG 1 cut(s) 798
Bme18I GGWCC 1 cut(s) 995
BmeT110I CYCGRG 1 cut(s) 1181
BmgT120I GGNCC 1 cut(s) 995
BmiI GGNNCC 1 cut(s) 959
BmrFI CCNGG 1 cut(s) 798
BmrI ACTGGG 1 cut(s) 311
BmsI GCATC 3 cut(s) 40, 401, 636
BmuI ACTGGG 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 169
BpmI CTGGAG 1 cut(s) 253
Bpu10I CCTNAGC 1 cut(s) 527
Bpu1102I GCTNAGC 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 672
BsaAI YACGTR 1 cut(s) 734
BsaBI GATNNNNATC 1 cut(s) 843
BsaHI GRCGYC 1 cut(s) 145
BsaJI CCNNGG 6 cut(s) 107, 260, 796, 991, 1001, 1150
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 323, 755
Bse118I RCCGGY 1 cut(s) 1105
Bse1I ACTGG 2 cut(s) 245, 317
Bse3DI GCAATG 1 cut(s) 552
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 1 cut(s) 798
BseDI CCNNGG 6 cut(s) 107, 260, 796, 991, 1001, 1150
BseGI GGATG 1 cut(s) 627
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 3 cut(s) 322, 323, 755
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 4 cut(s) 30, 257, 341, 518
BseNI ACTGG 2 cut(s) 245, 317
BseXI GCAGC 1 cut(s) 611
BseYI CCCAGC 1 cut(s) 85
Bsh1236I CGCG 3 cut(s) 337, 339, 584
Bsh1285I CGRYCG 1 cut(s) 453
BshFI GGCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 453
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 1181
BsiSI CCGG 1 cut(s) 1106
BslFI GGGAC 2 cut(s) 725, 737
BslI CCNNNNNNNGG 3 cut(s) 322, 323, 755
BsmAI GTCTC 2 cut(s) 152, 262
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 2 cut(s) 725, 737
BsmI GAATGC 2 cut(s) 116, 118
BsnI GGCC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 1181
Bsp1286I GDGCHC 1 cut(s) 200
Bsp1407I TGTACA 1 cut(s) 867
Bsp143I GATC 2 cut(s) 446, 858
Bsp1720I GCTNAGC 1 cut(s) 39
Bsp19I CCATGG 2 cut(s) 991, 1001
BspACI CCGC 5 cut(s) 126, 339, 357, 371, 1170
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 4 cut(s) 31, 258, 342, 519
BspFNI CGCG 3 cut(s) 337, 339, 584
BspHI TCATGA 1 cut(s) 1080
BspLI GGNNCC 1 cut(s) 959
BspMAI CTGCAG 2 cut(s) 680, 907
BspPI GGATC 1 cut(s) 454
BspQI GCTCTTC 1 cut(s) 205
BsrDI GCAATG 1 cut(s) 552
BsrFI RCCGGY 1 cut(s) 1105
BsrGI TGTACA 1 cut(s) 867
BsrI ACTGG 2 cut(s) 245, 317
BssAI RCCGGY 1 cut(s) 1105
BssECI CCNNGG 6 cut(s) 107, 260, 796, 991, 1001, 1150
BssMI GATC 2 cut(s) 446, 858
BssNAI GTATAC 1 cut(s) 774
BssNI GRCGYC 1 cut(s) 145
BssSI CACGAG 1 cut(s) 218
BssT1I CCWWGG 4 cut(s) 107, 991, 1001, 1150
Bst1107I GTATAC 1 cut(s) 774
Bst2BI CACGAG 1 cut(s) 218
Bst2UI CCWGG 1 cut(s) 798
Bst4CI ACNGT 7 cut(s) 388, 454, 610, 661, 778, 865, 1102
Bst6I CTCTTC 1 cut(s) 205
BstACI GRCGYC 1 cut(s) 145
BstAPI GCANNNNNTGC 1 cut(s) 497
BstAUI TGTACA 1 cut(s) 867
BstBAI YACGTR 1 cut(s) 734
BstC8I GCNNGC 9 cut(s) 94, 329, 480, 637, 680, 684, 750, 946, 985
BstDEI CTNAG 4 cut(s) 39, 266, 350, 527
BstDSI CCRYGG 2 cut(s) 991, 1001
BstF5I GGATG 1 cut(s) 627
BstFNI CGCG 3 cut(s) 337, 339, 584
BstHHI GCGC 4 cut(s) 339, 349, 584, 707
BstKTI GATC 2 cut(s) 449, 861
BstMAI GTCTC 2 cut(s) 152, 262
BstMBI GATC 2 cut(s) 446, 858
BstMCI CGRYCG 1 cut(s) 453
BstMWI GCNNNNNNNGC 5 cut(s) 214, 488, 497, 941, 1167
BstNI CCWGG 1 cut(s) 798
BstNSI RCATGY 2 cut(s) 167, 478
BstSCI CCNGG 1 cut(s) 796
BstSFI CTRYAG 2 cut(s) 676, 903
BstUI CGCG 3 cut(s) 337, 339, 584
BstV1I GCAGC 1 cut(s) 611
BstV2I GAAGAC 1 cut(s) 169
BstXI CCANNNNNNTGG 1 cut(s) 720
BstZ17I GTATAC 1 cut(s) 774
BsuI GTATCC 1 cut(s) 855
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 2 cut(s) 991, 1001
BtsCI GGATG 1 cut(s) 627
BtsI GCAGTG 2 cut(s) 673, 978
BtsIMutI CAGTG 4 cut(s) 673, 783, 870, 978
Cac8I GCNNGC 9 cut(s) 94, 329, 480, 637, 680, 684, 750, 946, 985
CaiI CAGNNNCTG 1 cut(s) 497
CciI TCATGA 1 cut(s) 1080
CfoI GCGC 4 cut(s) 339, 349, 584, 707
Cfr10I RCCGGY 1 cut(s) 1105
Cfr13I GGNCC 1 cut(s) 995
Csp6I GTAC 5 cut(s) 55, 592, 665, 868, 924
CviAII CATG 6 cut(s) 164, 475, 745, 992, 1002, 1081
CviQI GTAC 5 cut(s) 55, 592, 665, 868, 924
DdeI CTNAG 4 cut(s) 39, 266, 350, 527
DpnI GATC 2 cut(s) 448, 860
DpnII GATC 2 cut(s) 446, 858
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
Ecl136II GAGCTC 1 cut(s) 198
Eco130I CCWWGG 4 cut(s) 107, 991, 1001, 1150
Eco24I GRGCYC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 995
Eco53kI GAGCTC 1 cut(s) 198
Eco57I CTGAAG 1 cut(s) 489
Eco88I CYCGRG 1 cut(s) 1181
EcoICRI GAGCTC 1 cut(s) 198
EcoRI GAATTC 1 cut(s) 586
EcoRII CCWGG 1 cut(s) 796
EcoT14I CCWWGG 4 cut(s) 107, 991, 1001, 1150
EcoT22I ATGCAT 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 4 cut(s) 107, 991, 1001, 1150
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 6 cut(s) 167, 478, 748, 995, 1005, 1084
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FaqI GGGAC 2 cut(s) 725, 737
FatI CATG 6 cut(s) 163, 474, 744, 991, 1001, 1080
FauI CCCGC 1 cut(s) 350
FbaI TGATCA 1 cut(s) 858
FblI GTMKAC 1 cut(s) 773
Fnu4HI GCNGC 1 cut(s) 625
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 1 cut(s) 625
FspI TGCGCA 1 cut(s) 706
GlaI GCGC 4 cut(s) 338, 348, 583, 706
GluI GCNGC 1 cut(s) 625
GsaI CCCAGC 1 cut(s) 89
GsuI CTGGAG 1 cut(s) 253
HaeIII GGCC 1 cut(s) 188
HapII CCGG 1 cut(s) 1106
HhaI GCGC 4 cut(s) 339, 349, 584, 707
Hin1I GRCGYC 1 cut(s) 145
Hin1II CATG 6 cut(s) 167, 478, 748, 995, 1005, 1084
Hin6I GCGC 4 cut(s) 337, 347, 582, 705
HinP1I GCGC 4 cut(s) 337, 347, 582, 705
HincII GTYRAC 1 cut(s) 900
HindII GTYRAC 1 cut(s) 900
HindIII AAGCTT 3 cut(s) 436, 458, 827
HinfI GANTC 4 cut(s) 256, 270, 781, 821
HpaII CCGG 1 cut(s) 1106
Hpy166II GTNNAC 3 cut(s) 774, 868, 900
Hpy188I TCNGA 5 cut(s) 156, 465, 1068, 1095, 1114
Hpy188III TCNNGA 6 cut(s) 220, 689, 818, 836, 1081, 1192
Hpy8I GTNNAC 3 cut(s) 774, 868, 900
HpyAV CCTTC 2 cut(s) 10, 166
HpyCH4III ACNGT 7 cut(s) 388, 454, 610, 661, 778, 865, 1102
HpyCH4IV ACGT 4 cut(s) 145, 726, 733, 770
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 488, 497, 941, 1167
HpyF3I CTNAG 4 cut(s) 39, 266, 350, 527
HpySE526I ACGT 4 cut(s) 145, 726, 733, 770
Hsp92I GRCGYC 1 cut(s) 145
Hsp92II CATG 6 cut(s) 167, 478, 748, 995, 1005, 1084
HspAI GCGC 4 cut(s) 337, 347, 582, 705
Ksp22I TGATCA 1 cut(s) 858
Kzo9I GATC 2 cut(s) 446, 858
LguI GCTCTTC 1 cut(s) 205
LmnI GCTCC 2 cut(s) 89, 963
Lsp1109I GCAGC 1 cut(s) 611
LweI GCATC 3 cut(s) 40, 401, 636
MaeII ACGT 4 cut(s) 145, 726, 733, 770
MaeIII GTNAC 5 cut(s) 236, 319, 382, 766, 778
MalI GATC 2 cut(s) 448, 860
MboI GATC 2 cut(s) 446, 858
MboII GAAGA 7 cut(s) 169, 192, 262, 495, 824, 905, 967
MfeI CAATTG 1 cut(s) 1131
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 4 cut(s) 516, 586, 716, 1131
MlyI GAGTC 3 cut(s) 264, 265, 775
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 6 cut(s) 270, 425, 522, 626, 998, 1008
Mph1103I ATGCAT 1 cut(s) 118
MroXI GAANNNNTTC 3 cut(s) 116, 176, 499
MseI TTAA 1 cut(s) 305
MslI CAYNNNNRTG 1 cut(s) 1040
MspI CCGG 1 cut(s) 1106
MspR9I CCNGG 1 cut(s) 798
MunI CAATTG 1 cut(s) 1131
Mva1269I GAATGC 2 cut(s) 116, 118
MvaI CCWGG 1 cut(s) 798
MvnI CGCG 3 cut(s) 337, 339, 584
MwoI GCNNNNNNNGC 5 cut(s) 214, 488, 497, 941, 1167
NcoI CCATGG 2 cut(s) 991, 1001
NdeII GATC 2 cut(s) 446, 858
NlaIII CATG 6 cut(s) 167, 478, 748, 995, 1005, 1084
NlaIV GGNNCC 1 cut(s) 959
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 2 cut(s) 382, 778
NsbI TGCGCA 1 cut(s) 706
NsiI ATGCAT 1 cut(s) 118
NspI RCATGY 2 cut(s) 167, 478
PagI TCATGA 1 cut(s) 1080
PciI ACATGT 1 cut(s) 163
PciSI GCTCTTC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 606
PctI GAATGC 2 cut(s) 116, 118
PdmI GAANNNNTTC 3 cut(s) 116, 176, 499
PfeI GAWTC 1 cut(s) 821
PflFI GACNNNGTC 1 cut(s) 784
PflMI CCANNNNNTGG 1 cut(s) 755
PkrI GCNGC 1 cut(s) 626
PleI GAGTC 3 cut(s) 264, 264, 775
PpsI GAGTC 3 cut(s) 264, 264, 775
Ppu21I YACGTR 1 cut(s) 734
PscI ACATGT 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 796
PspFI CCCAGC 1 cut(s) 85
PspGI CCWGG 1 cut(s) 796
PspN4I GGNNCC 1 cut(s) 959
PspPI GGNCC 1 cut(s) 995
PstI CTGCAG 2 cut(s) 680, 907
PstNI CAGNNNCTG 1 cut(s) 497
PsyI GACNNNGTC 1 cut(s) 784
RsaI GTAC 5 cut(s) 56, 593, 666, 869, 925
RsaNI GTAC 5 cut(s) 55, 592, 665, 868, 924
RseI CAYNNNNRTG 1 cut(s) 1040
SacI GAGCTC 1 cut(s) 200
SapI GCTCTTC 1 cut(s) 205
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 1 cut(s) 625
Sau3AI GATC 2 cut(s) 446, 858
Sau96I GGNCC 1 cut(s) 995
ScaI AGTACT 1 cut(s) 925
SchI GAGTC 3 cut(s) 264, 265, 775
ScrFI CCNGG 1 cut(s) 798
SduI GDGCHC 1 cut(s) 200
SfaNI GCATC 3 cut(s) 40, 401, 636
SfcI CTRYAG 2 cut(s) 676, 903
SinI GGWCC 1 cut(s) 995
SmiMI CAYNNNNRTG 1 cut(s) 1040
SmlI CTYRAG 1 cut(s) 687
SmoI CTYRAG 1 cut(s) 687
Sse9I AATT 4 cut(s) 516, 586, 716, 1131
SsiI CCGC 5 cut(s) 126, 339, 357, 371, 1170
SstI GAGCTC 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 796
StyI CCWWGG 4 cut(s) 107, 991, 1001, 1150
TaaI ACNGT 7 cut(s) 388, 454, 610, 661, 778, 865, 1102
TaiI ACGT 4 cut(s) 148, 729, 736, 773
TaqI TCGA 2 cut(s) 449, 788
TasI AATT 4 cut(s) 516, 586, 716, 1131
TatI WGTACW 2 cut(s) 867, 923
TfiI GAWTC 1 cut(s) 821
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 4 cut(s) 680, 783, 870, 985
TseFI GTSAC 2 cut(s) 382, 778
TseI GCWGC 1 cut(s) 624
Tsp45I GTSAC 2 cut(s) 382, 778
TspDTI ATGAA 3 cut(s) 65, 109, 586
TspRI CASTG 4 cut(s) 680, 783, 870, 985
Tth111I GACNNNGTC 1 cut(s) 784
Van91I CCANNNNNTGG 1 cut(s) 755
VpaK11BI GGWCC 1 cut(s) 995
XapI RAATTY 2 cut(s) 586, 716
XceI RCATGY 2 cut(s) 167, 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 324
XmiI GTMKAC 1 cut(s) 773
XmnI GAANNNNTTC 3 cut(s) 116, 176, 499
ZraI GACGTC 1 cut(s) 146
ZrmI AGTACT 1 cut(s) 925
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.