pycom15g15370

Phospholipase D alpha 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010738
Physical Location & Seq
Reverse (-)
10700091 .. 10701325
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1140 bp
ATGCTTGTGTGGGATGACAGAACTTCAGTGAAGTTGCTAAAGAGAGATGGAGTGATGGCTACTCATGATGAAGACACTGAAAGTTACTTCCACAACACAGCGGTTCATTGCGTGTTGTGTCCCCGAAACCCTGACAACGGGAATAGCATTGTCAATGATTTACAGGTGTCCACCATGTTTACGCATCACCAGAAGATTGTCGTGGTGGACAGTGAATTGCCTAAGGGAGGAGGGTTGGAAAAGAGGAGGATTGTGAGCTTCATTGGTGGCATTGATCTTTGTGATGGAAGATATGACACACCTTCACATCCCATTTTCAGGACTTTAGACACAACACACAAGAATGATCTCCATCAGCCTAATTTTGGGGGCGTTGATCGATCACAAAAGCAAGGTGGGAAGGACTTGCTTGTTGAACTCAGAGAGCTTGATGACACATTTATACCACCTTCTCCGGTTATGTCCCCTCAAGACCATGATACATGGAATGTTCAGCTGTTTCGCTCCATCGATGGAGGAGCTGCATTTGGATTCCCTGACTCCCCCGAAGATGCTGCCAAGGCAGGCCTTGTCACCGGGAAAAATCACGTGATTGATCGAAGCATTCAGGATGATTACATCAATGCCATTCGGTGCGCGAAGTCTTTTATCTACATCGAAAATCAGTACTTTCTTGGAAGCTCTTTTGGATGGCATTCGGATTATGTTACCCTAAAAGTTAAGGAGGTTGGTGCTTTGCATCTGATCCCAAAGGAACTGTCACTAAAGATTGTTAGTAAGATTGAAGCTGGAGAAAGATTTACGGTTTACATTGTGATGCCAATGTGGCCAGAGGGCATTCCGGAAAGCCAAACCGCTCAGGCAATGTTGCATTGGCAGAGGATGACGATGGACATGATGTATAGGGACATTGTTCAAACTCTCAAAGTCAAGGGGATTGAGGCAAACCCTAAGGATTATTTGGCATTCTTTTGCCTTGGTAACCACGAGAAGAAGCTACCCGGAGAATATGAACCTCCGGAAAAACCAGAACATGGATCAGATTACAGTCGAGCACAACAAGCAAGGCGGTTCATGATCTATGTTCATGCCAAGGTGATGATAGGTAGGCCAATCTTATATCACATTCTTCTTAATTAA

Protein Analysis

380

Amino Acids

43.04

Weight (kDa)

6.09

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLDc PF00614 59 - 97 4.9e-07 Phospholipase D Active site motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010738)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25370 AT5G25370 AT5G25370 AT5G25370
fragaria_vesca FvH4_1g03170
malus_domestica MD02G1030700.v1.1 MD15G1172300.v1.1
prunus_persica Prupe.7G243800_v2.0.a1
pyrus_communis pycom02g02560 pycom15g15370
rosa_chinensis RchiOBHm_Chr2g0088301
rosa_laevigata RLG00000015940
rosa_multiflora Rmu_sc0004235.1_g000036
rosa_roxburghii Rroxscaffold_2G00152760
rosa_rugosa Rorug01G0482700
rosa_samantha Rh2AG037800 Rh2BG037000 Rh2CG038100 Rh2DG037500
rosa_wichuraiana Rw2G002980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1034
AccBSI CCGCTC 1 cut(s) 857
AccII CGCG 1 cut(s) 638
AccIII TCCGGA 2 cut(s) 841, 1018
AciI CCGC 3 cut(s) 101, 855, 1069
AclWI GGATC 2 cut(s) 739, 1045
AcoI YGGCCR 1 cut(s) 827
AcuI CTGAAG 1 cut(s) 9
AcvI CACGTG 1 cut(s) 589
AfaI GTAC 1 cut(s) 668
AfiI CCNNNNNNNGG 5 cut(s) 137, 227, 318, 365, 1034
AgsI TTSAA 3 cut(s) 416, 785, 917
AluBI AGCT 7 cut(s) 258, 427, 496, 521, 681, 788, 997
AluI AGCT 7 cut(s) 258, 427, 496, 521, 681, 788, 997
Alw21I GWGCWC 1 cut(s) 1057
AlwI GGATC 2 cut(s) 739, 1045
Aor13HI TCCGGA 2 cut(s) 841, 1018
AoxI GGCC 3 cut(s) 565, 827, 1109
ApeKI GCWGC 2 cut(s) 521, 554
ArsI GACNNNNNNTTYG 2 cut(s) 743, 775
AspLEI GCGC 1 cut(s) 638
AsuC2I CCSGG 2 cut(s) 577, 1002
AsuHPI GGTGA 3 cut(s) 179, 565, 1108
AxyI CCTNAGG 2 cut(s) 222, 951
BalI TGGCCA 1 cut(s) 829
BauI CACGAG 1 cut(s) 986
BbrPI CACGTG 1 cut(s) 589
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 1057
BbvI GCAGC 2 cut(s) 508, 541
BccI CCATC 8 cut(s) 41, 49, 278, 360, 506, 515, 684, 883
BcgI CGANNNNNNTGC 2 cut(s) 536, 570
BcnI CCSGG 2 cut(s) 577, 1002
BglI GCCNNNNNGGC 1 cut(s) 826
BisI GCNGC 2 cut(s) 522, 555
BlsI GCNGC 2 cut(s) 523, 556
BmcAI AGTACT 1 cut(s) 668
Bme1390I CCNGG 2 cut(s) 577, 1002
BmrFI CCNGG 2 cut(s) 577, 1002
BmsI GCATC 4 cut(s) 193, 541, 748, 807
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 810
Bpu10I CCTNAGC 1 cut(s) 858
BpuEI CTTGAG 1 cut(s) 453
BpuMI CCSGG 2 cut(s) 577, 1002
Bsa29I ATCGAT 2 cut(s) 379, 510
BsaAI YACGTR 1 cut(s) 589
BsaBI GATNNNNATC 1 cut(s) 351
BsaJI CCNNGG 3 cut(s) 558, 976, 1092
BsaWI WCCGGW 3 cut(s) 454, 841, 1018
Bsc4I CCNNNNNNNGG 5 cut(s) 137, 227, 318, 365, 1034
Bse21I CCTNAGG 2 cut(s) 222, 951
Bse3DI GCAATG 2 cut(s) 106, 870
Bse8I GATNNNNATC 1 cut(s) 351
BseAI TCCGGA 2 cut(s) 841, 1018
BseCI ATCGAT 2 cut(s) 379, 510
BseDI CCNNGG 3 cut(s) 558, 976, 1092
BseGI GGATG 5 cut(s) 19, 307, 616, 695, 888
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 5 cut(s) 137, 227, 318, 365, 1034
BseMI GCAATG 2 cut(s) 106, 870
BseMII CTCAG 2 cut(s) 433, 872
BseRI GAGGAG 3 cut(s) 243, 259, 531
BseXI GCAGC 2 cut(s) 508, 541
Bsh1236I CGCG 1 cut(s) 638
BshFI GGCC 3 cut(s) 567, 829, 1111
BshVI ATCGAT 2 cut(s) 379, 510
BsiHKAI GWGCWC 1 cut(s) 1057
BsiSI CCGG 5 cut(s) 455, 576, 842, 1002, 1019
BslFI GGGAC 3 cut(s) 105, 448, 920
BslI CCNNNNNNNGG 5 cut(s) 137, 227, 318, 365, 1034
BsmFI GGGAC 3 cut(s) 105, 448, 920
BsmI GAATGC 4 cut(s) 603, 694, 837, 965
BsnI GGCC 3 cut(s) 567, 829, 1111
Bsp1286I GDGCHC 1 cut(s) 1057
Bsp13I TCCGGA 2 cut(s) 841, 1018
Bsp143I GATC 8 cut(s) 274, 346, 376, 380, 595, 744, 1037, 1077
BspACI CCGC 3 cut(s) 101, 855, 1069
BspANI GGCC 3 cut(s) 567, 829, 1111
BspCNI CTCAG 2 cut(s) 432, 871
BspDI ATCGAT 2 cut(s) 379, 510
BspEI TCCGGA 2 cut(s) 841, 1018
BspFNI CGCG 1 cut(s) 638
BspHI TCATGA 2 cut(s) 64, 1074
BspPI GGATC 2 cut(s) 739, 1045
BsrBI CCGCTC 1 cut(s) 857
BsrDI GCAATG 2 cut(s) 106, 870
BssECI CCNNGG 3 cut(s) 558, 976, 1092
BssMI GATC 8 cut(s) 274, 346, 376, 380, 595, 744, 1037, 1077
BssSI CACGAG 1 cut(s) 986
BssT1I CCWWGG 3 cut(s) 558, 976, 1092
Bst2BI CACGAG 1 cut(s) 986
Bst4CI ACNGT 4 cut(s) 212, 759, 805, 1049
BstBAI YACGTR 1 cut(s) 589
BstC8I GCNNGC 1 cut(s) 565
BstDEI CTNAG 4 cut(s) 222, 419, 858, 951
BstEII GGTNACC 1 cut(s) 980
BstENI CCTNNNNNAGG 1 cut(s) 225
BstF5I GGATG 5 cut(s) 19, 307, 616, 695, 888
BstFNI CGCG 1 cut(s) 638
BstHHI GCGC 1 cut(s) 638
BstKTI GATC 8 cut(s) 277, 349, 379, 383, 598, 747, 1040, 1080
BstMBI GATC 8 cut(s) 274, 346, 376, 380, 595, 744, 1037, 1077
BstMWI GCNNNNNNNGC 3 cut(s) 560, 826, 1061
BstPI GGTNACC 1 cut(s) 980
BstSCI CCNGG 2 cut(s) 575, 1000
BstUI CGCG 1 cut(s) 638
BstV1I GCAGC 2 cut(s) 508, 541
BstV2I GAAGAC 1 cut(s) 78
Bsu15I ATCGAT 2 cut(s) 379, 510
Bsu36I CCTNAGG 2 cut(s) 222, 951
BsuRI GGCC 3 cut(s) 567, 829, 1111
BsuTUI ATCGAT 2 cut(s) 379, 510
BtsCI GGATG 5 cut(s) 19, 307, 616, 695, 888
BtsIMutI CAGTG 3 cut(s) 33, 75, 217
Cac8I GCNNGC 1 cut(s) 565
CciI TCATGA 2 cut(s) 64, 1074
CfoI GCGC 1 cut(s) 638
ClaI ATCGAT 2 cut(s) 379, 510
Csp6I GTAC 1 cut(s) 667
CviAII CATG 8 cut(s) 65, 175, 476, 483, 895, 1034, 1075, 1088
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 4 cut(s) 222, 419, 858, 951
DpnI GATC 8 cut(s) 276, 348, 378, 382, 597, 746, 1039, 1079
DpnII GATC 8 cut(s) 274, 346, 376, 380, 595, 744, 1037, 1077
EaeI YGGCCR 1 cut(s) 827
Eco130I CCWWGG 3 cut(s) 558, 976, 1092
Eco147I AGGCCT 1 cut(s) 567
Eco57I CTGAAG 1 cut(s) 9
Eco72I CACGTG 1 cut(s) 589
Eco81I CCTNAGG 2 cut(s) 222, 951
Eco91I GGTNACC 1 cut(s) 980
EcoNI CCTNNNNNAGG 1 cut(s) 225
EcoO65I GGTNACC 1 cut(s) 980
EcoT14I CCWWGG 3 cut(s) 558, 976, 1092
ErhI CCWWGG 3 cut(s) 558, 976, 1092
FaeI CATG 8 cut(s) 68, 178, 479, 486, 898, 1037, 1078, 1091
FaqI GGGAC 3 cut(s) 105, 448, 920
FatI CATG 8 cut(s) 64, 174, 475, 482, 894, 1033, 1074, 1087
Fnu4HI GCNGC 2 cut(s) 522, 555
FokI GGATG 5 cut(s) 26, 294, 623, 702, 895
Fsp4HI GCNGC 2 cut(s) 522, 555
GlaI GCGC 1 cut(s) 637
GluI GCNGC 2 cut(s) 522, 555
GsuI CTGGAG 1 cut(s) 810
HaeIII GGCC 3 cut(s) 567, 829, 1111
HapII CCGG 5 cut(s) 455, 576, 842, 1002, 1019
HhaI GCGC 1 cut(s) 638
Hin1II CATG 8 cut(s) 68, 178, 479, 486, 898, 1037, 1078, 1091
Hin6I GCGC 1 cut(s) 636
HinP1I GCGC 1 cut(s) 636
HinfI GANTC 2 cut(s) 531, 539
HpaII CCGG 5 cut(s) 455, 576, 842, 1002, 1019
HphI GGTGA 3 cut(s) 179, 565, 1108
Hpy166II GTNNAC 4 cut(s) 171, 180, 208, 808
Hpy188I TCNGA 4 cut(s) 422, 700, 744, 1042
Hpy188III TCNNGA 7 cut(s) 65, 319, 470, 608, 842, 1019, 1075
Hpy8I GTNNAC 4 cut(s) 171, 180, 208, 808
HpyAV CCTTC 3 cut(s) 312, 394, 459
HpyCH4III ACNGT 4 cut(s) 212, 759, 805, 1049
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 3 cut(s) 524, 739, 871
HpyF10VI GCNNNNNNNGC 3 cut(s) 560, 826, 1061
HpyF3I CTNAG 4 cut(s) 222, 419, 858, 951
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 8 cut(s) 68, 178, 479, 486, 898, 1037, 1078, 1091
HspAI GCGC 1 cut(s) 636
Kpn2I TCCGGA 2 cut(s) 841, 1018
Kzo9I GATC 8 cut(s) 274, 346, 376, 380, 595, 744, 1037, 1077
LmnI GCTCC 2 cut(s) 509, 518
Lsp1109I GCAGC 2 cut(s) 508, 541
LweI GCATC 4 cut(s) 193, 541, 748, 807
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 5 cut(s) 83, 571, 706, 759, 980
MalI GATC 8 cut(s) 276, 348, 378, 382, 597, 746, 1039, 1079
MbiI CCGCTC 1 cut(s) 857
MboI GATC 8 cut(s) 274, 346, 376, 380, 595, 744, 1037, 1077
MboII GAAGA 6 cut(s) 83, 205, 300, 560, 1003, 1121
MhlI GDGCHC 1 cut(s) 1057
MlsI TGGCCA 1 cut(s) 829
MluCI AATT 3 cut(s) 215, 361, 1135
MluNI TGGCCA 1 cut(s) 829
MlyI GAGTC 1 cut(s) 533
MmeI TCCRAC 1 cut(s) 216
Mox20I TGGCCA 1 cut(s) 829
MroI TCCGGA 2 cut(s) 841, 1018
MscI TGGCCA 1 cut(s) 829
MseI TTAA 3 cut(s) 720, 1134, 1138
MslI CAYNNNNRTG 2 cut(s) 342, 815
Msp20I TGGCCA 1 cut(s) 829
MspA1I CMGCKG 2 cut(s) 101, 496
MspI CCGG 5 cut(s) 455, 576, 842, 1002, 1019
MspR9I CCNGG 2 cut(s) 577, 1002
Mva1269I GAATGC 4 cut(s) 603, 694, 837, 965
MvnI CGCG 1 cut(s) 638
MwoI GCNNNNNNNGC 3 cut(s) 560, 826, 1061
NciI CCSGG 2 cut(s) 577, 1002
NdeII GATC 8 cut(s) 274, 346, 376, 380, 595, 744, 1037, 1077
NlaIII CATG 8 cut(s) 68, 178, 479, 486, 898, 1037, 1078, 1091
NmuCI GTSAC 2 cut(s) 571, 759
PacI TTAATTAA 1 cut(s) 1138
PagI TCATGA 2 cut(s) 64, 1074
PceI AGGCCT 1 cut(s) 567
PctI GAATGC 4 cut(s) 603, 694, 837, 965
PfeI GAWTC 1 cut(s) 531
PflMI CCANNNNNTGG 1 cut(s) 1034
PkrI GCNGC 2 cut(s) 523, 556
PleI GAGTC 1 cut(s) 533
PmaCI CACGTG 1 cut(s) 589
PmlI CACGTG 1 cut(s) 589
PpsI GAGTC 1 cut(s) 533
Ppu21I YACGTR 1 cut(s) 589
PspCI CACGTG 1 cut(s) 589
PspEI GGTNACC 1 cut(s) 980
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
RseI CAYNNNNRTG 2 cut(s) 342, 815
SaqAI TTAA 3 cut(s) 720, 1134, 1138
SatI GCNGC 2 cut(s) 522, 555
Sau3AI GATC 8 cut(s) 274, 346, 376, 380, 595, 744, 1037, 1077
ScaI AGTACT 1 cut(s) 668
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 2 cut(s) 577, 1002
SduI GDGCHC 1 cut(s) 1057
SfaNI GCATC 4 cut(s) 193, 541, 748, 807
SmiMI CAYNNNNRTG 2 cut(s) 342, 815
SmlI CTYRAG 1 cut(s) 468
SmoI CTYRAG 1 cut(s) 468
Sse9I AATT 3 cut(s) 215, 361, 1135
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 3 cut(s) 101, 855, 1069
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 2 cut(s) 575, 1000
StyI CCWWGG 3 cut(s) 558, 976, 1092
TaaI ACNGT 4 cut(s) 212, 759, 805, 1049
TaiI ACGT 1 cut(s) 591
TaqI TCGA 5 cut(s) 379, 510, 598, 657, 1051
TasI AATT 3 cut(s) 215, 361, 1135
TatI WGTACW 1 cut(s) 666
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 3 cut(s) 720, 1134, 1138
Tru9I TTAA 3 cut(s) 720, 1134, 1138
TscAI CASTG 3 cut(s) 33, 82, 217
TseFI GTSAC 2 cut(s) 571, 759
TseI GCWGC 2 cut(s) 521, 554
Tsp45I GTSAC 2 cut(s) 571, 759
TspDTI ATGAA 6 cut(s) 84, 95, 250, 1026, 1063, 1076
TspRI CASTG 3 cut(s) 33, 82, 217
Van91I CCANNNNNTGG 1 cut(s) 1034
XagI CCTNNNNNAGG 1 cut(s) 225
ZrmI AGTACT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.