pycom15g16030

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009140
Physical Location & Seq
Forward (+)
11166287 .. 11169201
2915 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
ATGGATAATAAGAAGAAGGTGGCGGTTCCGCTGGTTTGCCACGGTCATTCGCGTCCGGAGATCGATCTGTTTTACAGTCCGGTTACTCCTGATGGGTTCTTCCTCATCAGTGCCAGTAAGGATTCGAGTCCCATGCTGAGAAACGGAGAGACTGGAGATTGGATTGGAACGTTTGAAGGGCACAAAGGTGCAGTGTGGAGTTGCTGCTTGGATACTAATGCCCTACGTGCTGCAACTGCTTCTGCAGATTTCTCTGCGAAAGTTTGGGATGCATTATCAGGAGATGTGCTACACTCATTTGAACACAAGCATATTGTTCGAGCCTGTGCTTTTTCAGAGGATACACACCTTTTGCTAACCGGGGGAGTAGAGAAAGTTATCCGTATATATGATTTGAATCGTCCGGATGCCCCTCCAAGAGAAGTGGATAAATCCCCAGGTTCAGTCAGAACTGCTGCGTGGCTCCACAGTGACCAGACCATACTAAGTTCTTGTACTGATATGGGAGGTGTCAGATTATGGGATGTAAGAACTGGTAAAATAGTCCAAACACTGGAGACCAAATCATCTGTGACCAGTTCTGAAGTTAGTCAAGATGGTCGTTATATTACTACCGCTGATGGGTCGACTGTGAAGTTTTGGGATGCCAACTACTTTGGGTTGGTGAAGAGCTACAATATGCCGTGCACAGTAGAATCAGCTTCGCTGGAGCCAAAGTTTGGGAACAAATTCATTGCTGGGGGAGAAGATATGTGGGTTCATGTGTTTGATTTCCATACTGGTGAAGAGATCGCATGCAACAAGGGTCACCACGGTCCTGTTCACTGTCTGCGTTTCTCGCCCGGAGGGGAGTCATATGCCTCGGGATCTGAAGATGGAACCATAAGAATATGGCAGACCCCTTTGACTCCAGAAGAAACCGAAACACTGGTAGCAAATGGGCCAGTTGGAAACGTGAAAGTACCCGCAGAAGAGGTTTCTCGCAAGATTGAGGGCTTCCAGATCACGGACGAGGGGAAAGCGAAAGAGAAGGAAGAGGCGGCAGGGAATGAGTAA

Protein Analysis

352

Amino Acids

38.27

Weight (kDa)

5.34

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 10 - 298 3.3e-44 EIF3I
Beta-prop_WDR5 PF25175 29 - 226 2.9e-27 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 29 - 133 6e-13 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 30 - 134 7.2e-11 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 32 - 214 4.2e-13 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 34 - 227 2.8e-21 Prp19 WD40 domain
WD40_Gbeta PF25391 35 - 133 1.7e-06 G protein beta WD-40 repeat protein
WD40 PF00400 55 - 90 5.6e-06 WD domain, G-beta repeat
WDR55 PF24796 99 - 209 6.1e-09 WDR55
Beta-prop_TEP1_2nd PF25047 100 - 221 9.1e-12 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 102 - 300 9.1e-33 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 102 - 239 2e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 102 - 207 5.4e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 147 - 215 2.5e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 158 - 298 2.1e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 191 - 300 1.1e-08 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 197 - 300 1.3e-07 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 202 - 301 1.3e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 231 - 303 3.3e-10 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 237 - 299 2.8e-10 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 241 - 300 5.2e-11 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 242 - 311 3.6e-10 WDR5 beta-propeller domain
WD40 PF00400 264 - 298 6.3e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 553, 719
AccI GTMKAC 1 cut(s) 626
AccII CGCG 1 cut(s) 52
AccIII TCCGGA 2 cut(s) 55, 403
AciI CCGC 5 cut(s) 23, 29, 615, 966, 1040
AclI AACGTT 1 cut(s) 170
AclWI GGATC 1 cut(s) 874
AcsI RAATTY 1 cut(s) 728
AcuI CTGAAG 2 cut(s) 603, 891
AfaI GTAC 2 cut(s) 496, 963
AfiI CCNNNNNNNGG 4 cut(s) 553, 621, 719, 1006
AgsI TTSAA 3 cut(s) 176, 302, 397
AjnI CCWGG 1 cut(s) 436
AleI CACNNNNGTG 1 cut(s) 186
AluBI AGCT 2 cut(s) 672, 701
AluI AGCT 2 cut(s) 672, 701
Alw21I GWGCWC 1 cut(s) 689
Alw26I GTCTC 2 cut(s) 143, 551
Alw44I GTGCAC 1 cut(s) 685
AlwI GGATC 1 cut(s) 874
Ama87I CYCGRG 1 cut(s) 862
Aor13HI TCCGGA 2 cut(s) 55, 403
AoxI GGCC 1 cut(s) 941
ApaLI GTGCAC 1 cut(s) 685
ApeKI GCWGC 3 cut(s) 204, 230, 455
ApoI RAATTY 1 cut(s) 728
Asp700I GAANNNNTTC 1 cut(s) 728
AspS9I GGNCC 2 cut(s) 815, 941
AsuC2I CCSGG 2 cut(s) 361, 843
AsuHPI GGTGA 3 cut(s) 676, 794, 800
AvaI CYCGRG 1 cut(s) 862
AvaII GGWCC 1 cut(s) 815
BaeGI GKGCMC 2 cut(s) 183, 689
Bbv12I GWGCWC 1 cut(s) 689
BbvI GCAGC 3 cut(s) 191, 217, 442
BccI CCATC 4 cut(s) 86, 590, 614, 869
BceAI ACGGC 1 cut(s) 667
BcgI CGANNNNNNTGC 4 cut(s) 115, 149, 299, 333
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 2 cut(s) 205, 334
BcnI CCSGG 2 cut(s) 361, 843
BcoDI GTCTC 2 cut(s) 143, 551
BfmI CTRYAG 1 cut(s) 243
BfuI GTATCC 2 cut(s) 205, 334
BisI GCNGC 4 cut(s) 205, 231, 456, 1041
BlsI GCNGC 4 cut(s) 206, 232, 457, 1042
Bme1390I CCNGG 3 cut(s) 361, 438, 843
Bme18I GGWCC 1 cut(s) 815
BmeT110I CYCGRG 1 cut(s) 862
BmgT120I GGNCC 2 cut(s) 815, 941
BmiI GGNNCC 4 cut(s) 27, 464, 711, 880
BmrFI CCNGG 3 cut(s) 361, 438, 843
BmsI GCATC 3 cut(s) 259, 397, 634
BpmI CTGGAG 4 cut(s) 174, 575, 728, 894
BpuMI CCSGG 2 cut(s) 361, 843
Bsa29I ATCGAT 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 5 cut(s) 40, 360, 436, 811, 861
BsaWI WCCGGW 3 cut(s) 55, 79, 403
BsaXI ACNNNNNCTCC 2 cut(s) 701, 731
Bsc4I CCNNNNNNNGG 4 cut(s) 553, 621, 719, 1006
Bse1I ACTGG 8 cut(s) 114, 157, 538, 558, 576, 784, 933, 944
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 396
BseAI TCCGGA 2 cut(s) 55, 403
BseBI CCWGG 1 cut(s) 438
BseCI ATCGAT 1 cut(s) 63
BseDI CCNNGG 5 cut(s) 40, 360, 436, 811, 861
BseGI GGATG 4 cut(s) 274, 412, 529, 649
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 4 cut(s) 553, 621, 719, 1006
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 8 cut(s) 114, 157, 538, 558, 576, 784, 933, 944
BseSI GKGCMC 2 cut(s) 183, 689
BseXI GCAGC 3 cut(s) 191, 217, 442
BseYI CCCAGC 1 cut(s) 737
BsgI GTGCAG 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 52
BshFI GGCC 1 cut(s) 943
BshVI ATCGAT 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 689
BsiHKCI CYCGRG 1 cut(s) 862
BsiSI CCGG 5 cut(s) 56, 80, 360, 404, 843
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 4 cut(s) 553, 621, 719, 1006
BsmAI GTCTC 2 cut(s) 143, 551
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 1 cut(s) 943
Bso31I GGTCTC 1 cut(s) 551
BsoBI CYCGRG 1 cut(s) 862
Bsp1286I GDGCHC 2 cut(s) 183, 689
Bsp13I TCCGGA 2 cut(s) 55, 403
Bsp143I GATC 5 cut(s) 60, 64, 789, 866, 1002
BspACI CCGC 5 cut(s) 23, 29, 615, 966, 1040
BspANI GGCC 1 cut(s) 943
BspCNI CTCAG 1 cut(s) 129
BspDI ATCGAT 1 cut(s) 63
BspEI TCCGGA 2 cut(s) 55, 403
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 4 cut(s) 27, 464, 711, 880
BspMAI CTGCAG 1 cut(s) 247
BspPI GGATC 1 cut(s) 874
BspQI GCTCTTC 1 cut(s) 662
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 8 cut(s) 114, 157, 538, 558, 576, 784, 933, 944
BssECI CCNNGG 5 cut(s) 40, 360, 436, 811, 861
BssMI GATC 5 cut(s) 60, 64, 789, 866, 1002
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 7 cut(s) 44, 77, 470, 631, 691, 815, 827
Bst6I CTCTTC 4 cut(s) 662, 780, 966, 1029
BstBAI YACGTR 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 796
BstDEI CTNAG 2 cut(s) 137, 485
BstDSI CCRYGG 2 cut(s) 40, 811
BstEII GGTNACC 1 cut(s) 806
BstF5I GGATG 4 cut(s) 274, 412, 529, 649
BstFNI CGCG 1 cut(s) 52
BstKTI GATC 5 cut(s) 63, 67, 792, 869, 1005
BstMAI GTCTC 2 cut(s) 143, 551
BstMBI GATC 5 cut(s) 60, 64, 789, 866, 1002
BstMWI GCNNNNNNNGC 3 cut(s) 227, 236, 838
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 1 cut(s) 798
BstPI GGTNACC 1 cut(s) 806
BstSCI CCNGG 3 cut(s) 359, 436, 841
BstSFI CTRYAG 1 cut(s) 243
BstSLI GKGCMC 2 cut(s) 183, 689
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 3 cut(s) 191, 217, 442
BstX2I RGATCY 1 cut(s) 866
BstYI RGATCY 1 cut(s) 866
Bsu15I ATCGAT 1 cut(s) 63
BsuI GTATCC 2 cut(s) 205, 334
BsuRI GGCC 1 cut(s) 943
BsuTUI ATCGAT 1 cut(s) 63
BtgI CCRYGG 2 cut(s) 40, 811
BtsCI GGATG 4 cut(s) 274, 412, 529, 649
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 6 cut(s) 115, 198, 475, 551, 823, 926
Cac8I GCNNGC 1 cut(s) 796
Cfr13I GGNCC 2 cut(s) 815, 941
ClaI ATCGAT 1 cut(s) 63
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 495, 962
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 3 cut(s) 133, 761, 795
CviJI RGCY 7 cut(s) 323, 463, 672, 701, 712, 943, 996
CviKI_1 RGCY 7 cut(s) 323, 463, 672, 701, 712, 943, 996
CviQI GTAC 2 cut(s) 495, 962
DdeI CTNAG 2 cut(s) 137, 485
DpnI GATC 5 cut(s) 62, 66, 791, 868, 1004
DpnII GATC 5 cut(s) 60, 64, 789, 866, 1002
Eam1104I CTCTTC 4 cut(s) 662, 780, 966, 1029
EarI CTCTTC 4 cut(s) 662, 780, 966, 1029
Eco31I GGTCTC 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 815
Eco57I CTGAAG 2 cut(s) 603, 891
Eco88I CYCGRG 1 cut(s) 862
Eco91I GGTNACC 1 cut(s) 806
EcoO65I GGTNACC 1 cut(s) 806
EcoRII CCWGG 1 cut(s) 436
EcoT22I ATGCAT 1 cut(s) 274
FaeI CATG 3 cut(s) 136, 764, 798
FaqI GGGAC 1 cut(s) 114
FatI CATG 3 cut(s) 132, 760, 794
FauI CCCGC 1 cut(s) 973
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 1 cut(s) 626
Fnu4HI GCNGC 4 cut(s) 205, 231, 456, 1041
FokI GGATG 4 cut(s) 281, 419, 536, 656
Fsp4HI GCNGC 4 cut(s) 205, 231, 456, 1041
GluI GCNGC 4 cut(s) 205, 231, 456, 1041
GsaI CCCAGC 1 cut(s) 741
GsuI CTGGAG 4 cut(s) 174, 575, 728, 894
HaeIII GGCC 1 cut(s) 943
HapII CCGG 5 cut(s) 56, 80, 360, 404, 843
HgaI GACGC 1 cut(s) 41
Hin1II CATG 3 cut(s) 136, 764, 798
HincII GTYRAC 1 cut(s) 627
HindII GTYRAC 1 cut(s) 627
HinfI GANTC 6 cut(s) 122, 127, 397, 695, 851, 907
HpaII CCGG 5 cut(s) 56, 80, 360, 404, 843
HphI GGTGA 3 cut(s) 676, 794, 800
Hpy166II GTNNAC 3 cut(s) 627, 687, 823
Hpy188I TCNGA 5 cut(s) 337, 449, 515, 583, 871
Hpy188III TCNNGA 8 cut(s) 56, 89, 279, 404, 593, 864, 911, 1000
Hpy8I GTNNAC 3 cut(s) 627, 687, 823
HpyAV CCTTC 3 cut(s) 10, 170, 1024
HpyCH4III ACNGT 7 cut(s) 44, 77, 470, 631, 691, 815, 827
HpyCH4IV ACGT 3 cut(s) 170, 226, 954
HpyCH4V TGCA 6 cut(s) 191, 233, 245, 272, 687, 798
HpyF10VI GCNNNNNNNGC 3 cut(s) 227, 236, 838
HpyF3I CTNAG 2 cut(s) 137, 485
HpySE526I ACGT 3 cut(s) 170, 226, 954
Hsp92II CATG 3 cut(s) 136, 764, 798
Kpn2I TCCGGA 2 cut(s) 55, 403
Kzo9I GATC 5 cut(s) 60, 64, 789, 866, 1002
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 2 cut(s) 468, 709
Lsp1109I GCAGC 3 cut(s) 191, 217, 442
LweI GCATC 3 cut(s) 259, 397, 634
MaeII ACGT 3 cut(s) 170, 226, 954
MaeIII GTNAC 4 cut(s) 82, 470, 571, 806
MalI GATC 5 cut(s) 62, 66, 791, 868, 1004
MboI GATC 5 cut(s) 60, 64, 789, 866, 1002
MboII GAAGA 9 cut(s) 25, 91, 679, 758, 797, 884, 926, 983, 1046
MflI RGATCY 1 cut(s) 866
MhlI GDGCHC 2 cut(s) 183, 689
MluCI AATT 1 cut(s) 728
MlyI GAGTC 3 cut(s) 136, 860, 901
MmeI TCCRAC 1 cut(s) 928
Mph1103I ATGCAT 1 cut(s) 274
MroI TCCGGA 2 cut(s) 55, 403
MroXI GAANNNNTTC 1 cut(s) 728
MslI CAYNNNNRTG 2 cut(s) 186, 780
MspA1I CMGCKG 2 cut(s) 31, 617
MspI CCGG 5 cut(s) 56, 80, 360, 404, 843
MspR9I CCNGG 3 cut(s) 361, 438, 843
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 3 cut(s) 227, 236, 838
NciI CCSGG 2 cut(s) 361, 843
NdeI CATATG 1 cut(s) 856
NdeII GATC 5 cut(s) 60, 64, 789, 866, 1002
NlaIII CATG 3 cut(s) 136, 764, 798
NlaIV GGNNCC 4 cut(s) 27, 464, 711, 880
NmuCI GTSAC 3 cut(s) 470, 571, 806
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 1 cut(s) 798
OliI CACNNNNGTG 1 cut(s) 186
PaeI GCATGC 1 cut(s) 798
PciSI GCTCTTC 1 cut(s) 662
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 3 cut(s) 122, 397, 695
PflMI CCANNNNNTGG 2 cut(s) 553, 719
PkrI GCNGC 4 cut(s) 206, 232, 457, 1042
PleI GAGTC 3 cut(s) 135, 859, 901
PpsI GAGTC 3 cut(s) 135, 859, 901
Ppu21I YACGTR 1 cut(s) 227
Psp1406I AACGTT 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 436
PspEI GGTNACC 1 cut(s) 806
PspFI CCCAGC 1 cut(s) 737
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 4 cut(s) 27, 464, 711, 880
PspPI GGNCC 2 cut(s) 815, 941
PstI CTGCAG 1 cut(s) 247
PsuI RGATCY 1 cut(s) 866
RsaI GTAC 2 cut(s) 496, 963
RsaNI GTAC 2 cut(s) 495, 962
RseI CAYNNNNRTG 2 cut(s) 186, 780
SalI GTCGAC 1 cut(s) 625
SapI GCTCTTC 1 cut(s) 662
SatI GCNGC 4 cut(s) 205, 231, 456, 1041
Sau3AI GATC 5 cut(s) 60, 64, 789, 866, 1002
Sau96I GGNCC 2 cut(s) 815, 941
SchI GAGTC 3 cut(s) 136, 860, 901
ScrFI CCNGG 3 cut(s) 361, 438, 843
SduI GDGCHC 2 cut(s) 183, 689
SfaNI GCATC 3 cut(s) 259, 397, 634
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 815
SmiMI CAYNNNNRTG 2 cut(s) 186, 780
SphI GCATGC 1 cut(s) 798
Sse9I AATT 1 cut(s) 728
SsiI CCGC 5 cut(s) 23, 29, 615, 966, 1040
StyD4I CCNGG 3 cut(s) 359, 436, 841
TaaI ACNGT 7 cut(s) 44, 77, 470, 631, 691, 815, 827
TaiI ACGT 3 cut(s) 173, 229, 957
TaqI TCGA 4 cut(s) 63, 125, 319, 626
TasI AATT 1 cut(s) 728
TatI WGTACW 1 cut(s) 494
TauI GCSGC 1 cut(s) 1043
TfiI GAWTC 3 cut(s) 122, 397, 695
TscAI CASTG 6 cut(s) 115, 198, 475, 558, 830, 933
TseFI GTSAC 3 cut(s) 470, 571, 806
TseI GCWGC 3 cut(s) 204, 230, 455
Tsp45I GTSAC 3 cut(s) 470, 571, 806
TspDTI ATGAA 2 cut(s) 721, 749
TspGWI ACGGA 3 cut(s) 159, 371, 1022
TspRI CASTG 6 cut(s) 115, 198, 475, 558, 830, 933
Van91I CCANNNNNTGG 2 cut(s) 553, 719
VneI GTGCAC 1 cut(s) 685
VpaK11BI GGWCC 1 cut(s) 815
XapI RAATTY 1 cut(s) 728
XceI RCATGY 1 cut(s) 798
XmiI GTMKAC 1 cut(s) 626
XmnI GAANNNNTTC 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.