pycom15g20690

Rab proteins geranylgeranyltransferase component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009509
Physical Location & Seq
Forward (+)
14830205 .. 14833144
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1008 bp
ATGGATTCCTGTGACTGTGACCCCCATTTGCTGAGATCTCTCCTGGTCTTCAAGAACACCATCAACCAGGAGTTGATCAGGAGCTTGCATGTGTACGGATTGGATGAGGGGAAGAGAAATGAGGTGGAGAGGGAGTTTGTGTTCAGAGAAAATGGGTTGTATGTGGAGCTGTCAGCTGAGCCGGTTCTGAGACTGATGAAATTTCCGGCTTCCCAAGTGCTCCAAGGGCATGTCAATGGCTGCTGGGTCTGCATTTTAGCATTTCATGAAAATTCACCTCCTGATTTTACATGCATTCCCTCTGTTCTTTCAGTCTCCAGAAATCCAAAGTTGAAGACAATTCCAAGATTGTCTAATGATCGATCTGCCCCCACTGTTCAGATGATTTTTGAGTTGAGTTGCAAAACAGATGACAAAAAATCATTGCAAAGGTCACGGGAGGAAACGAGCCAAGGGAGTAATGACAGTAACCACAAATCAAGGAGGTGGCCGATACTTGATCTGGATCTGAACTCTCTTCCTAGCCCCGTCACAATGTCTGAATCATCTGAGGATCAAGAAAGTGGAAGAAGTTCACCAGGTATTACAGAGAAGAAAAGAAGGGCACCAAGTGGACGCATAGCAAGTATCTCTTTGTCAGATCTGGCAAAGTATTTTGATCTACCAATTGTGGAAGCTTCGAGAAATTTGAATGTTGGACTCACAGTACTTAAGAAGAAATGCAGAGAATTTGGTATTCCTCGGTGGCCACATAGGAAGATTAAATCGCTTGACAGTCTCATTCGTGATCTTCAGGAAGAGACGGAGATCCAACAGAAGGAGAACAAAGCCGCAGCCTTGGCGGTTCTAAAGAGGCAAAGAATGCTGGAGAGGGAAAGAGAAAGTATAGAGAGGAGACCCTTCTTGGAGATGAAGACCGAGACCAAAAGATTCCGGCAAGATGTTTTCAAGAGACGGCACAGAGCTAGAGCTCTTAGAAGCCAGGGCCTGTCTACGTCTGCCAATTAA

Protein Analysis

336

Amino Acids

38.48

Weight (kDa)

9.52

Isoelectric Point (pI)

71.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 209 - 257 1.9e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 604
AccI GTMKAC 1 cut(s) 992
AciI CCGC 2 cut(s) 831, 842
AclWI GGATC 3 cut(s) 513, 561, 802
AcoI YGGCCR 2 cut(s) 488, 746
AcsI RAATTY 4 cut(s) 200, 271, 685, 728
AcuI CTGAAG 1 cut(s) 776
AdeI CACNNNGTG 1 cut(s) 611
AfaI GTAC 2 cut(s) 95, 708
AfiI CCNNNNNNNGG 1 cut(s) 817
AflII CTTAAG 1 cut(s) 710
AgsI TTSAA 4 cut(s) 52, 334, 691, 949
AjnI CCWGG 4 cut(s) 42, 66, 577, 981
AloI GAACNNNNNNTCC 2 cut(s) 125, 157
AluBI AGCT 6 cut(s) 84, 169, 176, 677, 965, 971
AluI AGCT 6 cut(s) 84, 169, 176, 677, 965, 971
Alw21I GWGCWC 2 cut(s) 222, 973
Alw26I GTCTC 7 cut(s) 184, 319, 782, 794, 889, 914, 946
AlwI GGATC 3 cut(s) 513, 561, 802
AlwNI CAGNNNCTG 1 cut(s) 988
AoxI GGCC 3 cut(s) 488, 746, 985
ApeKI GCWGC 2 cut(s) 240, 833
ApoI RAATTY 4 cut(s) 200, 271, 685, 728
Asp700I GAANNNNTTC 1 cut(s) 571
AspS9I GGNCC 1 cut(s) 985
AsuHPI GGTGA 2 cut(s) 267, 567
BaeGI GKGCMC 1 cut(s) 607
BalI TGGCCA 1 cut(s) 748
BanI GGYRCC 1 cut(s) 604
BanII GRGCYC 1 cut(s) 973
BbsI GAAGAC 3 cut(s) 40, 341, 920
Bbv12I GWGCWC 2 cut(s) 222, 973
BbvI GCAGC 2 cut(s) 227, 845
BccI CCATC 1 cut(s) 68
BceAI ACGGC 1 cut(s) 971
BciT130I CCWGG 4 cut(s) 44, 68, 579, 983
BclI TGATCA 1 cut(s) 75
BcoDI GTCTC 7 cut(s) 184, 319, 782, 794, 889, 914, 946
BfaI CTAG 2 cut(s) 522, 966
BfrI CTTAAG 1 cut(s) 710
BglII AGATCT 2 cut(s) 35, 640
BisI GCNGC 3 cut(s) 241, 831, 834
BlpI GCTNAGC 1 cut(s) 177
BlsI GCNGC 3 cut(s) 242, 832, 835
BmcAI AGTACT 1 cut(s) 708
Bme1390I CCNGG 4 cut(s) 44, 68, 579, 983
BmgT120I GGNCC 1 cut(s) 985
BmiI GGNNCC 1 cut(s) 606
BmrFI CCNGG 4 cut(s) 44, 68, 579, 983
BpiI GAAGAC 3 cut(s) 40, 341, 920
BpmI CTGGAG 2 cut(s) 301, 887
Bpu1102I GCTNAGC 1 cut(s) 177
Bsa29I ATCGAT 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 504
BsaI GGTCTC 2 cut(s) 889, 914
BsaJI CCNNGG 5 cut(s) 223, 451, 740, 837, 982
BsaXI ACNNNNNCTCC 4 cut(s) 119, 125, 149, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 817
Bse118I RCCGGY 1 cut(s) 181
Bse3DI GCAATG 1 cut(s) 422
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 4 cut(s) 44, 68, 579, 983
BseCI ATCGAT 1 cut(s) 361
BseDI CCNNGG 5 cut(s) 223, 451, 740, 837, 982
BseGI GGATG 1 cut(s) 109
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 817
BseMI GCAATG 1 cut(s) 422
BseMII CTCAG 4 cut(s) 23, 168, 179, 540
BseRI GAGGAG 1 cut(s) 907
BseSI GKGCMC 1 cut(s) 607
BseXI GCAGC 2 cut(s) 227, 845
BseYI CCCAGC 1 cut(s) 243
BshFI GGCC 3 cut(s) 490, 748, 987
BshNI GGYRCC 1 cut(s) 604
BshVI ATCGAT 1 cut(s) 361
BsiHKAI GWGCWC 2 cut(s) 222, 973
BsiSI CCGG 3 cut(s) 182, 206, 934
BslI CCNNNNNNNGG 1 cut(s) 817
BsmAI GTCTC 7 cut(s) 184, 319, 782, 794, 889, 914, 946
BsmBI CGTCTC 2 cut(s) 794, 946
BsmI GAATGC 2 cut(s) 294, 867
BsnI GGCC 3 cut(s) 490, 748, 987
Bso31I GGTCTC 2 cut(s) 889, 914
Bsp1286I GDGCHC 3 cut(s) 222, 607, 973
Bsp1720I GCTNAGC 1 cut(s) 177
BspACI CCGC 2 cut(s) 831, 842
BspANI GGCC 3 cut(s) 490, 748, 987
BspCNI CTCAG 4 cut(s) 24, 169, 180, 541
BspDI ATCGAT 1 cut(s) 361
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 606
BspPI GGATC 3 cut(s) 513, 561, 802
BspT107I GGYRCC 1 cut(s) 604
BspTI CTTAAG 1 cut(s) 710
BspTNI GGTCTC 2 cut(s) 889, 914
BsrDI GCAATG 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 5 cut(s) 223, 451, 740, 837, 982
BssT1I CCWWGG 3 cut(s) 223, 451, 837
Bst2UI CCWGG 4 cut(s) 44, 68, 579, 983
Bst4CI ACNGT 5 cut(s) 17, 376, 467, 706, 776
Bst6I CTCTTC 3 cut(s) 107, 522, 792
BstAFI CTTAAG 1 cut(s) 710
BstAPI GCANNNNNTGC 1 cut(s) 862
BstC8I GCNNGC 1 cut(s) 86
BstDEI CTNAG 5 cut(s) 32, 177, 188, 549, 974
BstF5I GGATG 1 cut(s) 109
BstMAI GTCTC 7 cut(s) 184, 319, 782, 794, 889, 914, 946
BstMWI GCNNNNNNNGC 4 cut(s) 226, 249, 839, 862
BstNI CCWGG 4 cut(s) 44, 68, 579, 983
BstNSI RCATGY 3 cut(s) 92, 233, 294
BstSCI CCNGG 4 cut(s) 42, 66, 577, 981
BstSLI GKGCMC 1 cut(s) 607
BstV1I GCAGC 2 cut(s) 227, 845
BstV2I GAAGAC 3 cut(s) 40, 341, 920
BstX2I RGATCY 4 cut(s) 35, 505, 640, 807
BstYI RGATCY 4 cut(s) 35, 505, 640, 807
Bsu15I ATCGAT 1 cut(s) 361
BsuRI GGCC 3 cut(s) 490, 748, 987
BsuTUI ATCGAT 1 cut(s) 361
BtsCI GGATG 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 1 cut(s) 86
CaiI CAGNNNCTG 1 cut(s) 988
CciI TCATGA 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 985
ClaI ATCGAT 1 cut(s) 361
CseI GACGC 1 cut(s) 624
CsiI ACCWGGT 1 cut(s) 577
Csp6I GTAC 2 cut(s) 94, 707
CviAII CATG 4 cut(s) 89, 230, 266, 291
CviQI GTAC 2 cut(s) 94, 707
DdeI CTNAG 5 cut(s) 32, 177, 188, 549, 974
DraIII CACNNNGTG 1 cut(s) 611
EaeI YGGCCR 2 cut(s) 488, 746
Eam1104I CTCTTC 3 cut(s) 107, 522, 792
EarI CTCTTC 3 cut(s) 107, 522, 792
Ecl136II GAGCTC 1 cut(s) 971
Eco130I CCWWGG 3 cut(s) 223, 451, 837
Eco24I GRGCYC 1 cut(s) 973
Eco31I GGTCTC 2 cut(s) 889, 914
Eco53kI GAGCTC 1 cut(s) 971
Eco57I CTGAAG 1 cut(s) 776
EcoICRI GAGCTC 1 cut(s) 971
EcoO109I RGGNCCY 1 cut(s) 985
EcoRII CCWGG 4 cut(s) 42, 66, 577, 981
EcoT14I CCWWGG 3 cut(s) 223, 451, 837
EcoT22I ATGCAT 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 973
ErhI CCWWGG 3 cut(s) 223, 451, 837
Esp3I CGTCTC 2 cut(s) 794, 946
FaeI CATG 4 cut(s) 92, 233, 269, 294
FaiI YATR 8 cut(s) 90, 162, 231, 267, 292, 620, 753, 887
FalI AAGNNNNNCTT 2 cut(s) 616, 648
FatI CATG 4 cut(s) 88, 229, 265, 290
FbaI TGATCA 1 cut(s) 75
FblI GTMKAC 1 cut(s) 992
Fnu4HI GCNGC 3 cut(s) 241, 831, 834
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 973
Fsp4HI GCNGC 3 cut(s) 241, 831, 834
FspBI CTAG 2 cut(s) 522, 966
GluI GCNGC 3 cut(s) 241, 831, 834
GsaI CCCAGC 1 cut(s) 247
GsuI CTGGAG 2 cut(s) 301, 887
HaeIII GGCC 3 cut(s) 490, 748, 987
HapII CCGG 3 cut(s) 182, 206, 934
HgaI GACGC 1 cut(s) 624
Hin1II CATG 4 cut(s) 92, 233, 269, 294
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 4 cut(s) 5, 542, 699, 930
HpaII CCGG 3 cut(s) 182, 206, 934
HphI GGTGA 2 cut(s) 267, 567
Hpy166II GTNNAC 4 cut(s) 94, 575, 614, 993
Hpy188I TCNGA 7 cut(s) 146, 189, 381, 510, 541, 550, 640
Hpy8I GTNNAC 4 cut(s) 94, 575, 614, 993
HpyAV CCTTC 3 cut(s) 594, 811, 910
HpyCH4III ACNGT 5 cut(s) 17, 376, 467, 706, 776
HpyCH4IV ACGT 1 cut(s) 995
HpyCH4V TGCA 6 cut(s) 88, 252, 294, 402, 427, 723
HpyF10VI GCNNNNNNNGC 4 cut(s) 226, 249, 839, 862
HpyF3I CTNAG 5 cut(s) 32, 177, 188, 549, 974
HpySE526I ACGT 1 cut(s) 995
Hsp92II CATG 4 cut(s) 92, 233, 269, 294
Ksp22I TGATCA 1 cut(s) 75
LmnI GCTCC 3 cut(s) 81, 166, 225
Lsp1109I GCAGC 2 cut(s) 227, 845
MabI ACCWGGT 1 cut(s) 577
MaeI CTAG 2 cut(s) 522, 966
MaeII ACGT 1 cut(s) 995
MaeIII GTNAC 5 cut(s) 11, 17, 432, 467, 529
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 4 cut(s) 35, 505, 640, 807
MhlI GDGCHC 3 cut(s) 222, 607, 973
MlsI TGGCCA 1 cut(s) 748
MluCI AATT 7 cut(s) 200, 271, 339, 666, 685, 728, 1003
MluNI TGGCCA 1 cut(s) 748
MlyI GAGTC 1 cut(s) 693
MmeI TCCRAC 2 cut(s) 676, 835
Mox20I TGGCCA 1 cut(s) 748
Mph1103I ATGCAT 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 571
MscI TGGCCA 1 cut(s) 748
MseI TTAA 3 cut(s) 711, 762, 1006
MslI CAYNNNNRTG 1 cut(s) 234
Msp20I TGGCCA 1 cut(s) 748
MspA1I CMGCKG 1 cut(s) 176
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 3 cut(s) 182, 206, 934
MspR9I CCNGG 4 cut(s) 44, 68, 579, 983
MunI CAATTG 1 cut(s) 666
Mva1269I GAATGC 2 cut(s) 294, 867
MvaI CCWGG 4 cut(s) 44, 68, 579, 983
MwoI GCNNNNNNNGC 4 cut(s) 226, 249, 839, 862
NlaIII CATG 4 cut(s) 92, 233, 269, 294
NlaIV GGNNCC 1 cut(s) 606
NmuCI GTSAC 4 cut(s) 11, 17, 432, 529
NsiI ATGCAT 1 cut(s) 296
NspI RCATGY 3 cut(s) 92, 233, 294
PagI TCATGA 1 cut(s) 265
PctI GAATGC 2 cut(s) 294, 867
PdmI GAANNNNTTC 1 cut(s) 571
PfeI GAWTC 3 cut(s) 5, 542, 930
PkrI GCNGC 3 cut(s) 242, 832, 835
PleI GAGTC 1 cut(s) 693
PpsI GAGTC 1 cut(s) 693
Psp124BI GAGCTC 1 cut(s) 973
Psp6I CCWGG 4 cut(s) 42, 66, 577, 981
PspFI CCCAGC 1 cut(s) 243
PspGI CCWGG 4 cut(s) 42, 66, 577, 981
PspN4I GGNNCC 1 cut(s) 606
PspPI GGNCC 1 cut(s) 985
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 4 cut(s) 35, 505, 640, 807
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 2 cut(s) 95, 708
RsaNI GTAC 2 cut(s) 94, 707
RseI CAYNNNNRTG 1 cut(s) 234
SacI GAGCTC 1 cut(s) 973
SaqAI TTAA 3 cut(s) 711, 762, 1006
SatI GCNGC 3 cut(s) 241, 831, 834
Sau96I GGNCC 1 cut(s) 985
ScaI AGTACT 1 cut(s) 708
SchI GAGTC 1 cut(s) 693
ScrFI CCNGG 4 cut(s) 44, 68, 579, 983
SduI GDGCHC 3 cut(s) 222, 607, 973
SexAI ACCWGGT 1 cut(s) 577
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
Sse9I AATT 7 cut(s) 200, 271, 339, 666, 685, 728, 1003
SsiI CCGC 2 cut(s) 831, 842
SspMI CTAG 2 cut(s) 522, 966
SstI GAGCTC 1 cut(s) 973
StyD4I CCNGG 4 cut(s) 42, 66, 577, 981
StyI CCWWGG 3 cut(s) 223, 451, 837
TaaI ACNGT 5 cut(s) 17, 376, 467, 706, 776
TaiI ACGT 1 cut(s) 998
TaqI TCGA 2 cut(s) 361, 680
TaqII GACCGA 1 cut(s) 932
TasI AATT 7 cut(s) 200, 271, 339, 666, 685, 728, 1003
TatI WGTACW 1 cut(s) 706
TauI GCSGC 1 cut(s) 833
TfiI GAWTC 3 cut(s) 5, 542, 930
Tru1I TTAA 3 cut(s) 711, 762, 1006
Tru9I TTAA 3 cut(s) 711, 762, 1006
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 4 cut(s) 11, 17, 432, 529
TseI GCWGC 2 cut(s) 240, 833
Tsp45I GTSAC 4 cut(s) 11, 17, 432, 529
TspDTI ATGAA 4 cut(s) 212, 254, 282, 926
TspGWI ACGGA 2 cut(s) 111, 818
TspRI CASTG 1 cut(s) 379
Vha464I CTTAAG 1 cut(s) 710
XapI RAATTY 4 cut(s) 200, 271, 685, 728
XceI RCATGY 3 cut(s) 92, 233, 294
XmiI GTMKAC 1 cut(s) 992
XmnI GAANNNNTTC 1 cut(s) 571
XspI CTAG 2 cut(s) 522, 966
ZrmI AGTACT 1 cut(s) 708
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.