pycom15g21740

negative regulation of mRNA metabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011510
Physical Location & Seq
Forward (+)
15783924 .. 15788335
4412 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2976 bp
ATGGGGAGCCGTCATATTGTTTCGAGATGGGTATCTGGGCTTGACCCTGGGCCTCAGGCAAAACCTACTTTATTGAGCTTTTTTGAGCTTTCTTATCTTGAACGCGTAGCTCTCCATTTAGCAAACTCGAAAGACAAAACCTCATTCGCGTCGAAACTGAAAACTCCACCGTGCTCCGCTACTCACGGTCAGATTCACAAACTCCGACAACCAAACCCCTATCTCCACTTCCCTCTCAATTCCTTCGGGTTTTATCCGCTGCATATCCTAAGCATGAGACCTCGCAAGGCGGACGCTTCCAAACCGCCGACCGCTAAGAAAACCCCGCCGGCGAGGAAAACCGCCGCTAAAACACAACCGGCGGCTTCCCCGCCGGACTTAGAAGTGGCCACTCTGAAAACCGTCGAAACAAAGCGCGGTTGTGCCGGCCGACTGATGCTGGCCAGGAAGAATGAAACGACACCGCCTGCTGATTCGAAGGCCCCCAAATCATCAGCAGCTGAGGGGACCGAGGGATCAGCTGGTACTGCTAAAGTAGCACCGGCGGCCAAAGTTGCTGTCGTGAAAAAAGTTCCTGCGAAAAGGTTGCCGGCCAAAGTTAAGACACTGGCATCTGCTAAATTTGTTTCACCGCAGACACCGAAATTGGGGGCATCAGTGAAGGCGAAAGCAGATGCATTGAACAAGGGGAAAGCCGCAGATGTTAGTGACGTGGAAGTGATAGAAAAGAAAGCGGTTGCTGAGAATGTTGGAGAGTCTGCTAAGAAGGATGGAAGCATTGTGGAATTTGAAGTAAGCACTGTTGAGAATGTTGGACAGCCTGGAACAACCAAACAAACAACCGTGGAAGTAAAAGTAAACCCGAATGAAGATGAAGAGGATCCTGAAGAGGAAGAGGATCCTGTTGAGGAGGAGGATCCTATCGAGGAAGAGGATCCCTTTGAGGCAGAAAAATCTGTAGCTGGGGAGGTTTCAGAGTTTTCTGAGAATGAACTAGCTTGTGAGGTAAAGGAGGGAGTGGAAGTGATTGAGGAAGAGGATCCCATTGAGGCAGAAAAATCTTTAGTTGGGGAGGTTTCAAAGTCTTCTGAGAATGAACCATCTCATGATGTAAAAGAGGGAGTGGAGGTGAAAGAAGGTCAGAAGGAGATGTCGGAGAGAGTAGAAGTGAAAGGAGATCAGAAGGAGGAGTCGGAGGATAATGGAGATGAAGTCGAGACCTGTAGTGAGGGTGCTAACAAGGTAGAAAATTCCAAAGAGGGGTTACAAGGAGAAGATGTTGAGCATGGTAAAGGGGTGTATGATGGTGATGAGCAGATGGAAGAGTATGGTGAGAAATTGGATCTTGGAGAACATGGAGAGGAGGAACTTCCAGAGGATGATGCAGAGGATCCCGCAGAAGAAACTAAGAGATTGGCTGATGAACATAAGGAATTTACAGCCCTTGCGAATGAGCGCAAGATGAGGAAAGAACGTGAGATATTTCTTGGTGGGCTCGATCGCACCCAGGATGCTGTGGAGGAAGATGTGAGAAGGGTCTTTGAGAGGATTGGAGGGATAGTTGAAGTTAGGTTACACAAGAATCCTTCAACTAATAGGCACAAGGGTTATGCATTTGTGGAGTTTGAAAATAAGGAGTATGCCAGGCGTGCTTTGTCTGAAATGAAAAACCCCGTTATAAGAGGGAAGCGATGTGGGACAGCACCTAGTGAGGACAATGACACATTGTTCGTGGGTAATATATGCAATACCTGGACAAAGGAAGCTATTAAACAGAAACTGAAGGATTATGGTGTGGAAGGTGTTGAGAATATAAATCTTGTTCCAGATGTTCAAAGTGATGGGTTGAGCCGCGGTTTTGCATTTATTGAATTCTCTTGTCGTGGTGATGCTATGCTTGCATACAAGAGGCTTCAACAGCCTGATGCTATATTTGGCCACGCTGAAAGAACTGCCAAAGTGGCTTTTGCTGAACCTATACGTGAGCCTGACCCGGAGATCATGTCCCAAGTGAAGTCTGTATTTGTCGATGGGCTTCCACCTCCTTGGGATGAGGACAGAGTTAGAGAGCAATTTAAATGCTATGGAGAAATCTTACGCGTTGTTGTGGCCCGGAATATGTCTACAGCCAAGCGGAAGGATTTTGGATTTGTTGATTTCTCTACCCATGATTCTGCTGTGGCCTTTGTTGATAGTATAAATAACATAGAACTGGTGGATGGGAATTCAAAGGTAAAAGTGAAAGCAAGACTTTCAAATGCTTTGCCTAAAACTCAGGCTGTGAAGGGTGGAATGTGCGGTGGATTTCGAATTGGTCGTGGTGGTGGTGGTAGTGGTATCTTTTCAAGATCTGGACGGGGTTTTGGTCGCGGTGGACATCATTTCAACCGTGCAAAGTTCCAACCTGACAGACATTTCTATCGTGGTGGACGTGGTCAAACTGGCAGGATGGGTTTCCCGAATGAATATGACTTCGATTACCCATACGAGTTTCATGGACGAGGGGGAAGAAGGGGCTCTTTCAGGGACGGTCGTTATGGATTTGGTGGAGGTGCTTCAGTTGCTGCCCCACCATCAAGACCCTACACAGATCGACCTTGGCATGATGCCCCTGATAGAGGCTATGGTATGCATATTCCTCCTAGGAGGCATCCATATTCTCCAGGACAGTTTGATGGACCATTCATGGGGGGTCATTTTGATGAACCTTATTTTTACAATGATAACGCACATGGAATAAAACGTCCATTTCACATGAGAGACTATGATCATGACTACTCGGAGCCTAGTAGTCGTCGCCCACGGTTGGATTACATCGATCCAGCAGCTTCGTTTCATGGAAACCATTATAGGGATACTTACGGAGCTCGCAGCAGTGTCTACCCTCATGATTATTATGGTCCCGGATATAATTTCGGTCCATATTCATCTTATTTCGAGGGCAATCACTCATATGCAGGTGGCCGCTACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0006417 GO:0006950 GO:0006952 GO:0006955 GO:0007623 GO:0008143 GO:0008150 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016020 GO:0017148 GO:0019219 GO:0019222 GO:0030425 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031329 GO:0031330 GO:0032268 GO:0032269 GO:0032270 GO:0032991 GO:0034097 GO:0034248 GO:0034249 GO:0034250 GO:0034341 GO:0036477 GO:0042221 GO:0042995 GO:0043005 GO:0043025 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0043489 GO:0044297 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0045087 GO:0045727 GO:0045934 GO:0048027 GO:0048255 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0070717 GO:0070887 GO:0070934 GO:0070937 GO:0071013 GO:0071204 GO:0071310 GO:0071345 GO:0071346 GO:0080090 GO:0090365 GO:0090367 GO:0097159 GO:0097447 GO:0097452 GO:0097458 GO:0120025 GO:0120038 GO:1901363 GO:1902369 GO:1902373 GO:1902494 GO:1903311 GO:1903312 GO:1990635 GO:1990904 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

992

Amino Acids

109.75

Weight (kDa)

5.66

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 494 - 564 4.1e-16 RNA recognition motif
RRM_1 PF00076 576 - 640 1.5e-07 RNA recognition motif
RRM_1 PF00076 674 - 738 7.9e-12 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1679
AarI CACCTGC 1 cut(s) 2948
Acc36I ACCTGC 1 cut(s) 2948
AccI GTMKAC 2 cut(s) 2123, 2880
AccII CGCG 6 cut(s) 105, 149, 417, 1854, 2100, 2370
AcoI YGGCCR 7 cut(s) 387, 427, 441, 546, 591, 1936, 2962
AcsI RAATTY 6 cut(s) 620, 785, 1249, 1433, 1871, 2224
AcuI CTGAAG 3 cut(s) 906, 1802, 2541
AdeI CACNNNGTG 1 cut(s) 1709
AfaI GTAC 1 cut(s) 526
AfiI CCNNNNNNNGG 6 cut(s) 647, 1260, 2618, 2687, 2806, 2851
AflIII ACRYGT 2 cut(s) 103, 2098
AjiI CACGTC 2 cut(s) 712, 2432
AjnI CCWGG 7 cut(s) 46, 443, 820, 1506, 1643, 1751, 2662
Alw21I GWGCWC 2 cut(s) 176, 2869
Alw26I GTCTC 3 cut(s) 271, 1211, 2754
AlwNI CAGNNNCTG 3 cut(s) 500, 1780, 2564
ApeKI GCWGC 5 cut(s) 259, 497, 2564, 2825, 2871
ApoI RAATTY 6 cut(s) 620, 785, 1249, 1433, 1871, 2224
ArsI GACNNNNNNTTYG 4 cut(s) 543, 575, 1712, 1744
AspA2I CCTAGG 1 cut(s) 2642
AspLEI GCGC 2 cut(s) 417, 1458
AspS9I GGNCC 7 cut(s) 50, 481, 507, 2110, 2678, 2900, 2918
AsuC2I CCSGG 3 cut(s) 1994, 2113, 2904
AsuHPI GGTGA 5 cut(s) 621, 1141, 1319, 1343, 1898
AsuII TTCGAA 2 cut(s) 476, 2308
AvaII GGWCC 4 cut(s) 507, 2678, 2900, 2918
AvrII CCTAGG 1 cut(s) 2642
AxyI CCTNAGG 1 cut(s) 54
BalI TGGCCA 3 cut(s) 389, 443, 1938
BamHI GGATCC 6 cut(s) 880, 898, 916, 934, 1039, 1390
BanII GRGCYC 3 cut(s) 1497, 2519, 2869
BarI GAAGNNNNNNTAC 2 cut(s) 1557, 1589
BbsI GAAGAC 1 cut(s) 1077
Bbv12I GWGCWC 2 cut(s) 176, 2869
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 5 cut(s) 246, 509, 2551, 2837, 2883
BcgI CGANNNNNNTGC 2 cut(s) 568, 602
BciT130I CCWGG 7 cut(s) 48, 445, 822, 1508, 1645, 1753, 2664
BciVI GTATCC 1 cut(s) 2848
BclI TGATCA 1 cut(s) 2767
BcnI CCSGG 3 cut(s) 1994, 2113, 2904
BcoDI GTCTC 3 cut(s) 271, 1211, 2754
BfaI CTAG 4 cut(s) 995, 1707, 2643, 2787
BfmI CTRYAG 3 cut(s) 957, 1222, 2124
BfuAI ACCTGC 1 cut(s) 2948
BfuI GTATCC 1 cut(s) 2848
BglI GCCNNNNNGGC 1 cut(s) 1961
BglII AGATCT 1 cut(s) 2348
BlnI CCTAGG 1 cut(s) 2642
Bme18I GGWCC 4 cut(s) 507, 2678, 2900, 2918
BmgBI CACGTC 2 cut(s) 712, 2432
BmgT120I GGNCC 7 cut(s) 50, 481, 507, 2110, 2678, 2900, 2918
BpiI GAAGAC 1 cut(s) 1077
BpmI CTGGAG 1 cut(s) 2646
Bpu10I CCTNAGC 2 cut(s) 269, 501
Bpu14I TTCGAA 2 cut(s) 476, 2308
BpuMI CCSGG 3 cut(s) 1994, 2113, 2904
Bsa29I ATCGAT 1 cut(s) 2817
BsaAI YACGTR 1 cut(s) 1982
BsaBI GATNNNNATC 1 cut(s) 31
BsaI GGTCTC 2 cut(s) 271, 1211
Bsc4I CCNNNNNNNGG 6 cut(s) 647, 1260, 2618, 2687, 2806, 2851
Bse118I RCCGGY 5 cut(s) 328, 358, 425, 541, 589
Bse1I ACTGG 3 cut(s) 612, 2217, 2446
Bse21I CCTNAGG 1 cut(s) 54
Bse8I GATNNNNATC 1 cut(s) 31
BseBI CCWGG 7 cut(s) 48, 445, 822, 1508, 1645, 1753, 2664
BseCI ATCGAT 1 cut(s) 2817
BseGI GGATG 7 cut(s) 775, 1384, 1516, 2056, 2224, 2454, 2650
BseJI GATNNNNATC 1 cut(s) 31
BseLI CCNNNNNNNGG 6 cut(s) 647, 1260, 2618, 2687, 2806, 2851
BseMII CTCAG 6 cut(s) 68, 492, 732, 975, 1080, 2288
BseNI ACTGG 3 cut(s) 612, 2217, 2446
BseRI GAGGAG 4 cut(s) 923, 926, 1202, 1376
BseX3I CGGCCG 1 cut(s) 427
BseXI GCAGC 5 cut(s) 246, 509, 2551, 2837, 2883
BseYI CCCAGC 1 cut(s) 962
Bsh1236I CGCG 6 cut(s) 105, 149, 417, 1854, 2100, 2370
Bsh1285I CGRYCG 4 cut(s) 312, 430, 1501, 2533
BshVI ATCGAT 1 cut(s) 2817
BsiEI CGRYCG 4 cut(s) 312, 430, 1501, 2533
BsiHKAI GWGCWC 2 cut(s) 176, 2869
BsiSI CCGG 9 cut(s) 329, 359, 374, 426, 542, 590, 1994, 2113, 2904
BslFI GGGAC 5 cut(s) 520, 1711, 1990, 2540, 2886
BslI CCNNNNNNNGG 6 cut(s) 647, 1260, 2618, 2687, 2806, 2851
BsmAI GTCTC 3 cut(s) 271, 1211, 2754
BsmFI GGGAC 5 cut(s) 520, 1711, 1990, 2540, 2886
Bso31I GGTCTC 2 cut(s) 271, 1211
Bsp119I TTCGAA 2 cut(s) 476, 2308
Bsp1286I GDGCHC 4 cut(s) 176, 1497, 2519, 2869
BspCNI CTCAG 6 cut(s) 67, 493, 733, 976, 1081, 2287
BspDI ATCGAT 1 cut(s) 2817
BspFNI CGCG 6 cut(s) 105, 149, 417, 1854, 2100, 2370
BspHI TCATGA 3 cut(s) 1105, 2770, 2887
BspMI ACCTGC 1 cut(s) 2948
BspT104I TTCGAA 2 cut(s) 476, 2308
BspTNI GGTCTC 2 cut(s) 271, 1211
BsrFI RCCGGY 5 cut(s) 328, 358, 425, 541, 589
BsrI ACTGG 3 cut(s) 612, 2217, 2446
BssAI RCCGGY 5 cut(s) 328, 358, 425, 541, 589
BssT1I CCWWGG 3 cut(s) 2045, 2597, 2642
Bst2UI CCWGG 7 cut(s) 48, 445, 822, 1508, 1645, 1753, 2664
Bst4CI ACNGT 9 cut(s) 171, 188, 403, 802, 844, 2390, 2531, 2670, 2805
Bst6I CTCTTC 6 cut(s) 870, 882, 888, 924, 1029, 1317
BstBAI YACGTR 1 cut(s) 1982
BstBI TTCGAA 2 cut(s) 476, 2308
BstC8I GCNNGC 8 cut(s) 330, 427, 441, 468, 591, 1650, 1899, 2869
BstDSI CCRYGG 3 cut(s) 843, 1852, 2801
BstENI CCTNNNNNAGG 1 cut(s) 2616
BstF5I GGATG 7 cut(s) 775, 1384, 1516, 2056, 2224, 2454, 2650
BstFNI CGCG 6 cut(s) 105, 149, 417, 1854, 2100, 2370
BstHHI GCGC 2 cut(s) 417, 1458
BstMAI GTCTC 3 cut(s) 271, 1211, 2754
BstMCI CGRYCG 4 cut(s) 312, 430, 1501, 2533
BstMWI GCNNNNNNNGC 9 cut(s) 527, 536, 545, 554, 1649, 1898, 1918, 1961, 2561
BstNI CCWGG 7 cut(s) 48, 445, 822, 1508, 1645, 1753, 2664
BstSFI CTRYAG 3 cut(s) 957, 1222, 2124
BstUI CGCG 6 cut(s) 105, 149, 417, 1854, 2100, 2370
BstV1I GCAGC 5 cut(s) 246, 509, 2551, 2837, 2883
BstV2I GAAGAC 1 cut(s) 1077
BstX2I RGATCY 8 cut(s) 880, 898, 916, 934, 1039, 1342, 1390, 2348
BstXI CCANNNNNNTGG 1 cut(s) 2046
BstYI RGATCY 8 cut(s) 880, 898, 916, 934, 1039, 1342, 1390, 2348
BstZI CGGCCG 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 2817
Bsu36I CCTNAGG 1 cut(s) 54
BsuI GTATCC 1 cut(s) 2848
BsuTUI ATCGAT 1 cut(s) 2817
BtgI CCRYGG 3 cut(s) 843, 1852, 2801
BtgZI GCGATG 1 cut(s) 1705
BtrI CACGTC 2 cut(s) 712, 2432
BtsCI GGATG 7 cut(s) 775, 1384, 1516, 2056, 2224, 2454, 2650
BtsI GCAGTG 1 cut(s) 2881
BtsIMutI CAGTG 4 cut(s) 605, 663, 798, 2881
BveI ACCTGC 1 cut(s) 2948
Cac8I GCNNGC 8 cut(s) 330, 427, 441, 468, 591, 1650, 1899, 2869
CaiI CAGNNNCTG 3 cut(s) 500, 1780, 2564
CciI TCATGA 3 cut(s) 1105, 2770, 2887
CfoI GCGC 2 cut(s) 417, 1458
Cfr10I RCCGGY 5 cut(s) 328, 358, 425, 541, 589
Cfr13I GGNCC 7 cut(s) 50, 481, 507, 2110, 2678, 2900, 2918
Cfr42I CCGCGG 1 cut(s) 1855
ClaI ATCGAT 1 cut(s) 2817
CseI GACGC 2 cut(s) 138, 302
Csp6I GTAC 1 cut(s) 525
CviQI GTAC 1 cut(s) 525
DraI TTTAAA 1 cut(s) 2077
DraIII CACNNNGTG 1 cut(s) 1709
EaeI YGGCCR 7 cut(s) 387, 427, 441, 546, 591, 1936, 2962
EagI CGGCCG 1 cut(s) 427
Eam1104I CTCTTC 6 cut(s) 870, 882, 888, 924, 1029, 1317
EarI CTCTTC 6 cut(s) 870, 882, 888, 924, 1029, 1317
EciI GGCGGA 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 2867
EclXI CGGCCG 1 cut(s) 427
Eco130I CCWWGG 3 cut(s) 2045, 2597, 2642
Eco24I GRGCYC 3 cut(s) 1497, 2519, 2869
Eco31I GGTCTC 2 cut(s) 271, 1211
Eco47I GGWCC 4 cut(s) 507, 2678, 2900, 2918
Eco52I CGGCCG 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 2867
Eco57I CTGAAG 3 cut(s) 906, 1802, 2541
Eco81I CCTNAGG 1 cut(s) 54
EcoICRI GAGCTC 1 cut(s) 2867
EcoNI CCTNNNNNAGG 1 cut(s) 2616
EcoO109I RGGNCCY 1 cut(s) 481
EcoRI GAATTC 2 cut(s) 1871, 2224
EcoRII CCWGG 7 cut(s) 46, 443, 820, 1506, 1643, 1751, 2662
EcoT14I CCWWGG 3 cut(s) 2045, 2597, 2642
EcoT22I ATGCAT 3 cut(s) 679, 1615, 2634
EcoT38I GRGCYC 3 cut(s) 1497, 2519, 2869
ErhI CCWWGG 3 cut(s) 2045, 2597, 2642
FalI AAGNNNNNCTT 4 cut(s) 2235, 2267, 2503, 2535
FaqI GGGAC 5 cut(s) 520, 1711, 1990, 2540, 2886
FauI CCCGC 3 cut(s) 333, 378, 1402
FauNDI CATATG 1 cut(s) 2953
FbaI TGATCA 1 cut(s) 2767
FblI GTMKAC 2 cut(s) 2123, 2880
FokI GGATG 7 cut(s) 782, 1391, 1523, 2063, 2231, 2461, 2637
FriOI GRGCYC 3 cut(s) 1497, 2519, 2869
FspBI CTAG 4 cut(s) 995, 1707, 2643, 2787
GlaI GCGC 2 cut(s) 416, 1457
GsaI CCCAGC 1 cut(s) 966
GsuI CTGGAG 1 cut(s) 2646
HapII CCGG 9 cut(s) 329, 359, 374, 426, 542, 590, 1994, 2113, 2904
HgaI GACGC 2 cut(s) 138, 302
HhaI GCGC 2 cut(s) 417, 1458
Hin6I GCGC 2 cut(s) 415, 1456
HinP1I GCGC 2 cut(s) 415, 1456
HinfI GANTC 6 cut(s) 193, 473, 755, 1190, 1582, 2171
HpaII CCGG 9 cut(s) 329, 359, 374, 426, 542, 590, 1994, 2113, 2904
HphI GGTGA 5 cut(s) 621, 1141, 1319, 1343, 1898
Hpy166II GTNNAC 5 cut(s) 859, 2124, 2375, 2429, 2881
Hpy8I GTNNAC 5 cut(s) 859, 2124, 2375, 2429, 2881
Hpy99I CGWCG 3 cut(s) 154, 407, 2799
HpyCH4III ACNGT 9 cut(s) 171, 188, 403, 802, 844, 2390, 2531, 2670, 2805
HpyCH4IV ACGT 5 cut(s) 711, 1474, 1981, 2431, 2743
HpyF10VI GCNNNNNNNGC 9 cut(s) 527, 536, 545, 554, 1649, 1898, 1918, 1961, 2561
HpySE526I ACGT 5 cut(s) 711, 1474, 1981, 2431, 2743
HspAI GCGC 2 cut(s) 415, 1456
KroI GCCGGC 3 cut(s) 328, 425, 589
KroNI GCCGGC 3 cut(s) 330, 427, 591
Ksp22I TGATCA 1 cut(s) 2767
KspI CCGCGG 1 cut(s) 1855
LmnI GCTCC 4 cut(s) 6, 179, 2782, 2864
Lsp1109I GCAGC 5 cut(s) 246, 509, 2551, 2837, 2883
MaeI CTAG 4 cut(s) 995, 1707, 2643, 2787
MaeII ACGT 5 cut(s) 711, 1474, 1981, 2431, 2743
MaeIII GTNAC 3 cut(s) 707, 1263, 1572
MflI RGATCY 8 cut(s) 880, 898, 916, 934, 1039, 1342, 1390, 2348
MhlI GDGCHC 4 cut(s) 176, 1497, 2519, 2869
MlsI TGGCCA 3 cut(s) 389, 443, 1938
MluI ACGCGT 2 cut(s) 103, 2098
MluNI TGGCCA 3 cut(s) 389, 443, 1938
MlyI GAGTC 2 cut(s) 764, 1199
MmeI TCCRAC 7 cut(s) 229, 730, 793, 1134, 1173, 2425, 2787
Mox20I TGGCCA 3 cut(s) 389, 443, 1938
Mph1103I ATGCAT 3 cut(s) 679, 1615, 2634
MreI CGCCGGCG 1 cut(s) 328
MroNI GCCGGC 3 cut(s) 328, 425, 589
MscI TGGCCA 3 cut(s) 389, 443, 1938
MseI TTAA 4 cut(s) 600, 1770, 2076, 2974
MslI CAYNNNNRTG 2 cut(s) 2700, 2952
Msp20I TGGCCA 3 cut(s) 389, 443, 1938
MspA1I CMGCKG 4 cut(s) 259, 500, 521, 1854
MspI CCGG 9 cut(s) 329, 359, 374, 426, 542, 590, 1994, 2113, 2904
MvaI CCWGG 7 cut(s) 48, 445, 822, 1508, 1645, 1753, 2664
MvnI CGCG 6 cut(s) 105, 149, 417, 1854, 2100, 2370
MwoI GCNNNNNNNGC 9 cut(s) 527, 536, 545, 554, 1649, 1898, 1918, 1961, 2561
NaeI GCCGGC 3 cut(s) 330, 427, 591
NciI CCSGG 3 cut(s) 1994, 2113, 2904
NdeI CATATG 1 cut(s) 2953
NgoMIV GCCGGC 3 cut(s) 328, 425, 589
NmuCI GTSAC 1 cut(s) 707
NsiI ATGCAT 3 cut(s) 679, 1615, 2634
NspV TTCGAA 2 cut(s) 476, 2308
PagI TCATGA 3 cut(s) 1105, 2770, 2887
PaqCI CACCTGC 1 cut(s) 2948
PasI CCCWGGG 1 cut(s) 47
PcsI WCGNNNNNNNCGW 2 cut(s) 2314, 2428
PdiI GCCGGC 3 cut(s) 330, 427, 591
PfeI GAWTC 4 cut(s) 193, 473, 1582, 2171
PflFI GACNNNGTC 1 cut(s) 2433
PfoI TCCNGGA 2 cut(s) 2662, 2902
Ple19I CGATCG 1 cut(s) 1501
PleI GAGTC 2 cut(s) 763, 1198
PpsI GAGTC 2 cut(s) 763, 1198
Ppu21I YACGTR 1 cut(s) 1982
PsiI TTATAA 1 cut(s) 1679
Psp124BI GAGCTC 1 cut(s) 2869
Psp6I CCWGG 7 cut(s) 46, 443, 820, 1506, 1643, 1751, 2662
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 7 cut(s) 46, 443, 820, 1506, 1643, 1751, 2662
PspPI GGNCC 7 cut(s) 50, 481, 507, 2110, 2678, 2900, 2918
PstNI CAGNNNCTG 3 cut(s) 500, 1780, 2564
PsuI RGATCY 8 cut(s) 880, 898, 916, 934, 1039, 1342, 1390, 2348
PsyI GACNNNGTC 1 cut(s) 2433
PvuI CGATCG 1 cut(s) 1501
PvuII CAGCTG 2 cut(s) 500, 521
RsaI GTAC 1 cut(s) 526
RsaNI GTAC 1 cut(s) 525
RseI CAYNNNNRTG 2 cut(s) 2700, 2952
SacI GAGCTC 1 cut(s) 2869
SacII CCGCGG 1 cut(s) 1855
SaqAI TTAA 4 cut(s) 600, 1770, 2076, 2974
Sau96I GGNCC 7 cut(s) 50, 481, 507, 2110, 2678, 2900, 2918
SchI GAGTC 2 cut(s) 764, 1199
SduI GDGCHC 4 cut(s) 176, 1497, 2519, 2869
SfcI CTRYAG 3 cut(s) 957, 1222, 2124
Sfr303I CCGCGG 1 cut(s) 1855
SfuI TTCGAA 2 cut(s) 476, 2308
SgrAI CRCCGGYG 2 cut(s) 328, 541
SgrBI CCGCGG 1 cut(s) 1855
SinI GGWCC 4 cut(s) 507, 2678, 2900, 2918
SmiI ATTTAAAT 1 cut(s) 2077
SmiMI CAYNNNNRTG 2 cut(s) 2700, 2952
SspMI CTAG 4 cut(s) 995, 1707, 2643, 2787
SstI GAGCTC 1 cut(s) 2869
StyI CCWWGG 3 cut(s) 2045, 2597, 2642
SwaI ATTTAAAT 1 cut(s) 2077
TaaI ACNGT 9 cut(s) 171, 188, 403, 802, 844, 2390, 2531, 2670, 2805
TaiI ACGT 5 cut(s) 714, 1477, 1984, 2434, 2746
TaqII GACCGA 2 cut(s) 524, 2906
TauI GCSGC 6 cut(s) 347, 365, 548, 698, 1854, 2967
TfiI GAWTC 4 cut(s) 193, 473, 1582, 2171
Tru1I TTAA 4 cut(s) 600, 1770, 2076, 2974
Tru9I TTAA 4 cut(s) 600, 1770, 2076, 2974
TscAI CASTG 4 cut(s) 612, 663, 805, 2881
TseFI GTSAC 1 cut(s) 707
TseI GCWGC 5 cut(s) 259, 497, 2564, 2825, 2871
Tsp45I GTSAC 1 cut(s) 707
TspGWI ACGGA 1 cut(s) 2877
TspRI CASTG 4 cut(s) 612, 663, 805, 2881
Tth111I GACNNNGTC 1 cut(s) 2433
VpaK11BI GGWCC 4 cut(s) 507, 2678, 2900, 2918
XagI CCTNNNNNAGG 1 cut(s) 2616
XapI RAATTY 6 cut(s) 620, 785, 1249, 1433, 1871, 2224
XmaJI CCTAGG 1 cut(s) 2642
XmiI GTMKAC 2 cut(s) 2123, 2880
XspI CTAG 4 cut(s) 995, 1707, 2643, 2787
Zsp2I ATGCAT 3 cut(s) 679, 1615, 2634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.